TY  - JOUR
AU  - Velimirović, Milica
AU  - Jevtić Dožudić, Gordana
AU  - Selaković, Vesna
AU  - Stojković, Tihomir
AU  - Puškaš, Nela
AU  - Zaletel, Ivan
AU  - Živković, Milica
AU  - Dragutinović, Vesna
AU  - Nikolić, Tatjana
AU  - Jelenković, Ankica
AU  - Đorović, Đorđe
AU  - Mirčić, Aleksandar
AU  - Petronijević, Nataša D.
PY  - 2018
UR  - https://www.hindawi.com/journals/omcl/2018/3273654/
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC5932460
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3167
AB  - Decreased blood flow in the brain leads to a rapid increase in reactive oxygen species (ROS). NADPH oxidase (NOX) is an enzyme family that has the physiological function to produce ROS. NOX2 and NOX4 overexpression is associated with aggravated ischemic injury, while NOX2/4-deficient mice had reduced stroke size. Dysregulation of matrix metalloproteinases (MMPs) contributes to tissue damage. The active form of vitamin D3 expresses neuroprotective, immunomodulatory, and anti-inflammatory effects in the CNS. The present study examines the effects of the vitamin D3 pretreatment on the oxidative stress parameters and the expression of NOX subunits, MMP9, microglial marker Iba1, and vitamin D receptor (VDR), in the cortex and hippocampus of Mongolian gerbils subjected to ten minutes of global cerebral ischemia, followed by 24 hours of reperfusion. The ischemia/reperfusion procedure has induced oxidative stress, changes in the expression of NOX2 subunits and MMP9 in the brain, and increased MMP9 activity in the serum of experimental animals. Pretreatment with vitamin D3 was especially effective on NOX2 subunits, MMP9, and the level of malondialdehyde and superoxide anion. These results outline the significance of the NOX and MMP9 investigation in brain ischemia and the importance of adequate vitamin D supplementation in ameliorating the injury caused by I/R.
T2  - Oxidative medicine and cellular longevity
T1  - Effects of Vitamin D3 on the NADPH Oxidase and Matrix Metalloproteinase 9 in an Animal Model of Global Cerebral Ischemia.
VL  - 2018
DO  - 10.1155/2018/3273654
SP  - 3273654
ER  - 

@article{
author = "Velimirović, Milica and Jevtić Dožudić, Gordana and Selaković, Vesna and Stojković, Tihomir and Puškaš, Nela and Zaletel, Ivan and Živković, Milica and Dragutinović, Vesna and Nikolić, Tatjana and Jelenković, Ankica and Đorović, Đorđe and Mirčić, Aleksandar and Petronijević, Nataša D.",
year = "2018",
abstract = "Decreased blood flow in the brain leads to a rapid increase in reactive oxygen species (ROS). NADPH oxidase (NOX) is an enzyme family that has the physiological function to produce ROS. NOX2 and NOX4 overexpression is associated with aggravated ischemic injury, while NOX2/4-deficient mice had reduced stroke size. Dysregulation of matrix metalloproteinases (MMPs) contributes to tissue damage. The active form of vitamin D3 expresses neuroprotective, immunomodulatory, and anti-inflammatory effects in the CNS. The present study examines the effects of the vitamin D3 pretreatment on the oxidative stress parameters and the expression of NOX subunits, MMP9, microglial marker Iba1, and vitamin D receptor (VDR), in the cortex and hippocampus of Mongolian gerbils subjected to ten minutes of global cerebral ischemia, followed by 24 hours of reperfusion. The ischemia/reperfusion procedure has induced oxidative stress, changes in the expression of NOX2 subunits and MMP9 in the brain, and increased MMP9 activity in the serum of experimental animals. Pretreatment with vitamin D3 was especially effective on NOX2 subunits, MMP9, and the level of malondialdehyde and superoxide anion. These results outline the significance of the NOX and MMP9 investigation in brain ischemia and the importance of adequate vitamin D supplementation in ameliorating the injury caused by I/R.",
journal = "Oxidative medicine and cellular longevity",
title = "Effects of Vitamin D3 on the NADPH Oxidase and Matrix Metalloproteinase 9 in an Animal Model of Global Cerebral Ischemia.",
volume = "2018",
doi = "10.1155/2018/3273654",
pages = "3273654"
}

Velimirović, M., Jevtić Dožudić, G., Selaković, V., Stojković, T., Puškaš, N., Zaletel, I., Živković, M., Dragutinović, V., Nikolić, T., Jelenković, A., Đorović, Đ., Mirčić, A.,& Petronijević, N. D.. (2018). Effects of Vitamin D3 on the NADPH Oxidase and Matrix Metalloproteinase 9 in an Animal Model of Global Cerebral Ischemia.. in Oxidative medicine and cellular longevity, 2018, 3273654.
https://doi.org/10.1155/2018/3273654

Velimirović M, Jevtić Dožudić G, Selaković V, Stojković T, Puškaš N, Zaletel I, Živković M, Dragutinović V, Nikolić T, Jelenković A, Đorović Đ, Mirčić A, Petronijević ND. Effects of Vitamin D3 on the NADPH Oxidase and Matrix Metalloproteinase 9 in an Animal Model of Global Cerebral Ischemia.. in Oxidative medicine and cellular longevity. 2018;2018:3273654.
doi:10.1155/2018/3273654 .

Velimirović, Milica, Jevtić Dožudić, Gordana, Selaković, Vesna, Stojković, Tihomir, Puškaš, Nela, Zaletel, Ivan, Živković, Milica, Dragutinović, Vesna, Nikolić, Tatjana, Jelenković, Ankica, Đorović, Đorđe, Mirčić, Aleksandar, Petronijević, Nataša D., "Effects of Vitamin D3 on the NADPH Oxidase and Matrix Metalloproteinase 9 in an Animal Model of Global Cerebral Ischemia." in Oxidative medicine and cellular longevity, 2018 (2018):3273654,
https://doi.org/10.1155/2018/3273654 . .

TY  - JOUR
AU  - Mitrović, Marko
AU  - Tatalović, Nikola
AU  - Nikolić-Kokić, Aleksandra
AU  - Ciraj-Bjelac, Olivera
AU  - Krstić, Nikola
AU  - Oreščanin Dušić, Zorana
AU  - Krstić, Dragana
AU  - Jovanović, Zoran
AU  - Blagojević, Duško
AU  - Lazarević-Macanović, Mirjana
PY  - 2018
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641800029M
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/2841
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3226
AB  - In recent years, computed tomography (CT) has become very common in veterinary medicine. It is well known that testicles are organs with high radiosensitivity and their function can be impaired even after exposure to low radiation doses. In this work, we calculated the absorbed radiation doses after CT was performed with different voltage/current levels and correlated it with the activity of antioxidant enzymes in rabbit testicles. Two hours after CT, the activities of catalase (CAT) and glutathione peroxidase (GSH-Px) were increased in the testicles of animals that received an absorbed dose of 29.2 mGy. The same changes, along with elevated glutathione reductase (GR) activity, were observed after 7 days in animals that received the highest absorbed dose (46.3 mGy). It would appear that absorbed doses above 27.8 mGy provoked the antioxidant reaction but the time scale of the reaction was dose-dependent. Examination of the obtained results revealed that the main denominator of CT influence was a higher current. Our results suggest that CT influences the antioxidant status in rabbit testicles. The changes in antioxidant enzyme activities were dose- and time-dependent and influenced by the applied current.
T2  - Archives of Biological Sciences
T1  - Influence of absorbed radiation dose following computed tomography on the antioxidative status in rabbit testicles
IS  - 4
VL  - 70
DO  - 10.2298/ABS180413029M
SP  - 675
EP  - 680
ER  - 

@article{
author = "Mitrović, Marko and Tatalović, Nikola and Nikolić-Kokić, Aleksandra and Ciraj-Bjelac, Olivera and Krstić, Nikola and Oreščanin Dušić, Zorana and Krstić, Dragana and Jovanović, Zoran and Blagojević, Duško and Lazarević-Macanović, Mirjana",
year = "2018",
abstract = "In recent years, computed tomography (CT) has become very common in veterinary medicine. It is well known that testicles are organs with high radiosensitivity and their function can be impaired even after exposure to low radiation doses. In this work, we calculated the absorbed radiation doses after CT was performed with different voltage/current levels and correlated it with the activity of antioxidant enzymes in rabbit testicles. Two hours after CT, the activities of catalase (CAT) and glutathione peroxidase (GSH-Px) were increased in the testicles of animals that received an absorbed dose of 29.2 mGy. The same changes, along with elevated glutathione reductase (GR) activity, were observed after 7 days in animals that received the highest absorbed dose (46.3 mGy). It would appear that absorbed doses above 27.8 mGy provoked the antioxidant reaction but the time scale of the reaction was dose-dependent. Examination of the obtained results revealed that the main denominator of CT influence was a higher current. Our results suggest that CT influences the antioxidant status in rabbit testicles. The changes in antioxidant enzyme activities were dose- and time-dependent and influenced by the applied current.",
journal = "Archives of Biological Sciences",
title = "Influence of absorbed radiation dose following computed tomography on the antioxidative status in rabbit testicles",
number = "4",
volume = "70",
doi = "10.2298/ABS180413029M",
pages = "675-680"
}

Mitrović, M., Tatalović, N., Nikolić-Kokić, A., Ciraj-Bjelac, O., Krstić, N., Oreščanin Dušić, Z., Krstić, D., Jovanović, Z., Blagojević, D.,& Lazarević-Macanović, M.. (2018). Influence of absorbed radiation dose following computed tomography on the antioxidative status in rabbit testicles. in Archives of Biological Sciences, 70(4), 675-680.
https://doi.org/10.2298/ABS180413029M

Mitrović M, Tatalović N, Nikolić-Kokić A, Ciraj-Bjelac O, Krstić N, Oreščanin Dušić Z, Krstić D, Jovanović Z, Blagojević D, Lazarević-Macanović M. Influence of absorbed radiation dose following computed tomography on the antioxidative status in rabbit testicles. in Archives of Biological Sciences. 2018;70(4):675-680.
doi:10.2298/ABS180413029M .

Mitrović, Marko, Tatalović, Nikola, Nikolić-Kokić, Aleksandra, Ciraj-Bjelac, Olivera, Krstić, Nikola, Oreščanin Dušić, Zorana, Krstić, Dragana, Jovanović, Zoran, Blagojević, Duško, Lazarević-Macanović, Mirjana, "Influence of absorbed radiation dose following computed tomography on the antioxidative status in rabbit testicles" in Archives of Biological Sciences, 70, no. 4 (2018):675-680,
https://doi.org/10.2298/ABS180413029M . .

TY  - JOUR
AU  - Zaletel, Ivan
AU  - Schwirtlich, Marija
AU  - Perović, Milka
AU  - Jovanović, Mirna
AU  - Stevanović, Milena
AU  - Kanazir, Selma
AU  - Puškaš, Nela
PY  - 2018
UR  - http://www.medra.org/servlet/aliasResolver?alias=iospress&doi=10.3233/JAD-180277
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3149
AB  - Dysregulation of neurogenesis in the subgranular zone (SGZ) of the hippocampal dentate gyrus has been related to cognitive deficits and memory loss in neurodegenerative diseases, such as Alzheimer's disease (AD). Members of the B group of SOX transcription factors play critical roles in regulating neurogenesis in the embryonic and adult nervous system, including maintaining the multipotency, renewal, and cell fate decision of neural stem/progenitor cells. The aim of the present study was to evaluate the expression patterns of selected SOXB proteins in the SGZ, of 8-week-old male and female 5xFAD mice, which represent a transgenic model of AD with a severe and very early development of amyloid pathology. Immunohistochemical analysis showed a significant decrease in the number of cells expressing SOX1, SOX2, and SOX21 transcription factors within the SGZ of 5xFAD mice in comparison to their non-transgenic counterparts which coincidences with reduced number of doublecortin immunoreactive immature neurons found in Tg males. Despite observed changes in expressional pattern of examined SOXB proteins, the proliferative capacity evaluated by the number of Ki-67 immunoreactive cells remained unaffected in transgenic mice of both genders. Based on our results, we suggest that SOXB proteins might be considered as new biomarkers for the detection of early impairments in adult neurogenesis in different animal models or/and new targets in human regenerative medicine.
T2  - Journal of Alzheimer's Disease : JAD
T1  - Early Impairments of Hippocampal Neurogenesis in 5xFAD Mouse Model of Alzheimer's Disease Are Associated with Altered Expression of SOXB Transcription Factors.
IS  - 3
VL  - 65
DO  - 10.3233/JAD-180277
SP  - 963
EP  - 976
ER  - 

@article{
author = "Zaletel, Ivan and Schwirtlich, Marija and Perović, Milka and Jovanović, Mirna and Stevanović, Milena and Kanazir, Selma and Puškaš, Nela",
year = "2018",
abstract = "Dysregulation of neurogenesis in the subgranular zone (SGZ) of the hippocampal dentate gyrus has been related to cognitive deficits and memory loss in neurodegenerative diseases, such as Alzheimer's disease (AD). Members of the B group of SOX transcription factors play critical roles in regulating neurogenesis in the embryonic and adult nervous system, including maintaining the multipotency, renewal, and cell fate decision of neural stem/progenitor cells. The aim of the present study was to evaluate the expression patterns of selected SOXB proteins in the SGZ, of 8-week-old male and female 5xFAD mice, which represent a transgenic model of AD with a severe and very early development of amyloid pathology. Immunohistochemical analysis showed a significant decrease in the number of cells expressing SOX1, SOX2, and SOX21 transcription factors within the SGZ of 5xFAD mice in comparison to their non-transgenic counterparts which coincidences with reduced number of doublecortin immunoreactive immature neurons found in Tg males. Despite observed changes in expressional pattern of examined SOXB proteins, the proliferative capacity evaluated by the number of Ki-67 immunoreactive cells remained unaffected in transgenic mice of both genders. Based on our results, we suggest that SOXB proteins might be considered as new biomarkers for the detection of early impairments in adult neurogenesis in different animal models or/and new targets in human regenerative medicine.",
journal = "Journal of Alzheimer's Disease : JAD",
title = "Early Impairments of Hippocampal Neurogenesis in 5xFAD Mouse Model of Alzheimer's Disease Are Associated with Altered Expression of SOXB Transcription Factors.",
number = "3",
volume = "65",
doi = "10.3233/JAD-180277",
pages = "963-976"
}

Zaletel, I., Schwirtlich, M., Perović, M., Jovanović, M., Stevanović, M., Kanazir, S.,& Puškaš, N.. (2018). Early Impairments of Hippocampal Neurogenesis in 5xFAD Mouse Model of Alzheimer's Disease Are Associated with Altered Expression of SOXB Transcription Factors.. in Journal of Alzheimer's Disease : JAD, 65(3), 963-976.
https://doi.org/10.3233/JAD-180277

Zaletel I, Schwirtlich M, Perović M, Jovanović M, Stevanović M, Kanazir S, Puškaš N. Early Impairments of Hippocampal Neurogenesis in 5xFAD Mouse Model of Alzheimer's Disease Are Associated with Altered Expression of SOXB Transcription Factors.. in Journal of Alzheimer's Disease : JAD. 2018;65(3):963-976.
doi:10.3233/JAD-180277 .

Zaletel, Ivan, Schwirtlich, Marija, Perović, Milka, Jovanović, Mirna, Stevanović, Milena, Kanazir, Selma, Puškaš, Nela, "Early Impairments of Hippocampal Neurogenesis in 5xFAD Mouse Model of Alzheimer's Disease Are Associated with Altered Expression of SOXB Transcription Factors." in Journal of Alzheimer's Disease : JAD, 65, no. 3 (2018):963-976,
https://doi.org/10.3233/JAD-180277 . .