TY  - JOUR
AU  - Mihailović, Vladimir
AU  - Mišić, Danijela
AU  - Matić, Sanja
AU  - Mihailović, Mirjana
AU  - Stanić, Snežana
AU  - Vrvić, Miroslav M.
AU  - Katanić, Jelena
AU  - Mladenović, Milan
AU  - Stanković, Nevena
AU  - Boroja, Tatjana
AU  - Stanković, Milan S.
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2350
AB  - The purpose of this study was to evaluate the antioxidant potential of
   methanol extracts of Gentiana crudata L. aerial parts and roots, as well
   as the stability of the phenolic compounds and antioxidant capacity of
   extracts during heating, at different pHs and after an in vitro
   digestion procedure. Also, their genotoxicity and antigenotoxicity
   against carbon tetrachloride in the liver of albino Wistar rats using
   the comet assay were evaluated. Three secoiridoid glycosides
   (swertiamarin, gentiopicrin, and sweroside) and four phenolic compounds
   (orientin, vitexin and two isovitexin-glucosides) were identified as the
   major constituents in aerial parts and roots of G. cruciata, using
   UHPLC-DAD/+/- HESI-MS/MS analysis. The results of antioxidant assays
   showed that aerial parts displayed higher antioxidant activity compared
   to the roots, which could be related to higher phenolics content,
   especially flavonoids. In general, extracts showed pH and thermal
   stability, while duodenal condition had more influence on total phenolic
   condition and antioxidant activity of extracts. Both extracts showed a
   protective effect against CCl4 in comet assays. The roots extract showed
   no genotoxic activity, while aerial parts extract showed slight
   genotoxicity at concentrations of 400 mg/kg b.w. (C) 2015 Elsevier B.V.
   All rights reserved.
PB  - Elsevier
T2  - Industrial Crops and Products
T1  - Comparative phytochemical analysis of Gentiana cruciata L. roots and
 aerial parts, and their biological activities
VL  - 73
DO  - 10.1016/j.indcrop.2015.04.013
SP  - 49
EP  - 62
ER  - 

@article{
author = "Mihailović, Vladimir and Mišić, Danijela and Matić, Sanja and Mihailović, Mirjana and Stanić, Snežana and Vrvić, Miroslav M. and Katanić, Jelena and Mladenović, Milan and Stanković, Nevena and Boroja, Tatjana and Stanković, Milan S.",
year = "2015",
abstract = "The purpose of this study was to evaluate the antioxidant potential of
   methanol extracts of Gentiana crudata L. aerial parts and roots, as well
   as the stability of the phenolic compounds and antioxidant capacity of
   extracts during heating, at different pHs and after an in vitro
   digestion procedure. Also, their genotoxicity and antigenotoxicity
   against carbon tetrachloride in the liver of albino Wistar rats using
   the comet assay were evaluated. Three secoiridoid glycosides
   (swertiamarin, gentiopicrin, and sweroside) and four phenolic compounds
   (orientin, vitexin and two isovitexin-glucosides) were identified as the
   major constituents in aerial parts and roots of G. cruciata, using
   UHPLC-DAD/+/- HESI-MS/MS analysis. The results of antioxidant assays
   showed that aerial parts displayed higher antioxidant activity compared
   to the roots, which could be related to higher phenolics content,
   especially flavonoids. In general, extracts showed pH and thermal
   stability, while duodenal condition had more influence on total phenolic
   condition and antioxidant activity of extracts. Both extracts showed a
   protective effect against CCl4 in comet assays. The roots extract showed
   no genotoxic activity, while aerial parts extract showed slight
   genotoxicity at concentrations of 400 mg/kg b.w. (C) 2015 Elsevier B.V.
   All rights reserved.",
publisher = "Elsevier",
journal = "Industrial Crops and Products",
title = "Comparative phytochemical analysis of Gentiana cruciata L. roots and
 aerial parts, and their biological activities",
volume = "73",
doi = "10.1016/j.indcrop.2015.04.013",
pages = "49-62"
}

Mihailović, V., Mišić, D., Matić, S., Mihailović, M., Stanić, S., Vrvić, M. M., Katanić, J., Mladenović, M., Stanković, N., Boroja, T.,& Stanković, M. S.. (2015). Comparative phytochemical analysis of Gentiana cruciata L. roots and
 aerial parts, and their biological activities. in Industrial Crops and Products
Elsevier., 73, 49-62.
https://doi.org/10.1016/j.indcrop.2015.04.013

Mihailović V, Mišić D, Matić S, Mihailović M, Stanić S, Vrvić MM, Katanić J, Mladenović M, Stanković N, Boroja T, Stanković MS. Comparative phytochemical analysis of Gentiana cruciata L. roots and
 aerial parts, and their biological activities. in Industrial Crops and Products. 2015;73:49-62.
doi:10.1016/j.indcrop.2015.04.013 .

Mihailović, Vladimir, Mišić, Danijela, Matić, Sanja, Mihailović, Mirjana, Stanić, Snežana, Vrvić, Miroslav M., Katanić, Jelena, Mladenović, Milan, Stanković, Nevena, Boroja, Tatjana, Stanković, Milan S., "Comparative phytochemical analysis of Gentiana cruciata L. roots and
 aerial parts, and their biological activities" in Industrial Crops and Products, 73 (2015):49-62,
https://doi.org/10.1016/j.indcrop.2015.04.013 . .

TY  - CONF
AU  - Matić, Sanja
AU  - Stanić, Snežana
AU  - Bogojević, Desanka
AU  - Solujić, Slavica
AU  - Mladenović, Milan
AU  - Stanković, Nevena
AU  - Mihailović, Vladimir
AU  - Katanić, Jelena
AU  - Mihailović, Mirjana
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6141
AB  - The plant Cotinus coggygria Scop. (family Anacardiaceae) is commonly used in folk medicine for the treat­ment of various illnesses, such as diarrhea, paradontosis, gastric and duodenal ulcers. Different parts of this plant have been subjected to pharmacological evaluation for their potential antihaemorragic, wound-healing, anti­inflammatory, and antimicrobial activity. The present study was undertaken to investigate the phenolic conten according to the Folin-Ciocalteu procedure, while the spectrophotometric method with aluminium chloride was used for the determination of total ftavonoids. The phenolic and flavonoid composition of extract were deter­mined by the HPLC method. Antioxidant activiy was quantitatively determined using a DPPH radical scaveng­ing assay. To examine the antigenotoxic potential using the comet assay, Wistar rats were treated with 100 mg/ kg body weight of pyrogallol, which possesses a potent ability to generate free radicals and induce oxidative stress. The content of total phenols and ftavonoids was 3.78 mg of gallic acid/g of dry plant material and 8.29 mg of rutin/g of dry plant material, respectively. HPLC analysis showed that myricetin was the dominant compound (511.5 µg/g), while hydroxyl derivatives of cinnammic acids (chlorogenic, caffeic, coumaric, ferulic and rosmaric acid) were identified in the extract in varying amount. The neutralisation of DPPH radicals was up to 95%, while the maximum inhibition concentration is approximately 125 µg/ml. The dose of 500 mg/kg body weight of the extract, that display no genotoxic activity, and its main flavonoid compound myricetin, in equivalent amount as present in the extract, were applied intraperitoneally either 2 or 12 h prior to pyrogallol. As measured by the decrease in total score and tail moment, the DNA damage in liver was reduced by the extract and myricetin.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia
T1  - Chemical composition, antioxidant and antigenotoxic activities of Cotinus coggygria stem extract
SP  - 90
EP  - 91
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6141
ER  - 

@conference{
author = "Matić, Sanja and Stanić, Snežana and Bogojević, Desanka and Solujić, Slavica and Mladenović, Milan and Stanković, Nevena and Mihailović, Vladimir and Katanić, Jelena and Mihailović, Mirjana",
year = "2013",
abstract = "The plant Cotinus coggygria Scop. (family Anacardiaceae) is commonly used in folk medicine for the treat­ment of various illnesses, such as diarrhea, paradontosis, gastric and duodenal ulcers. Different parts of this plant have been subjected to pharmacological evaluation for their potential antihaemorragic, wound-healing, anti­inflammatory, and antimicrobial activity. The present study was undertaken to investigate the phenolic conten according to the Folin-Ciocalteu procedure, while the spectrophotometric method with aluminium chloride was used for the determination of total ftavonoids. The phenolic and flavonoid composition of extract were deter­mined by the HPLC method. Antioxidant activiy was quantitatively determined using a DPPH radical scaveng­ing assay. To examine the antigenotoxic potential using the comet assay, Wistar rats were treated with 100 mg/ kg body weight of pyrogallol, which possesses a potent ability to generate free radicals and induce oxidative stress. The content of total phenols and ftavonoids was 3.78 mg of gallic acid/g of dry plant material and 8.29 mg of rutin/g of dry plant material, respectively. HPLC analysis showed that myricetin was the dominant compound (511.5 µg/g), while hydroxyl derivatives of cinnammic acids (chlorogenic, caffeic, coumaric, ferulic and rosmaric acid) were identified in the extract in varying amount. The neutralisation of DPPH radicals was up to 95%, while the maximum inhibition concentration is approximately 125 µg/ml. The dose of 500 mg/kg body weight of the extract, that display no genotoxic activity, and its main flavonoid compound myricetin, in equivalent amount as present in the extract, were applied intraperitoneally either 2 or 12 h prior to pyrogallol. As measured by the decrease in total score and tail moment, the DNA damage in liver was reduced by the extract and myricetin.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia",
title = "Chemical composition, antioxidant and antigenotoxic activities of Cotinus coggygria stem extract",
pages = "90-91",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6141"
}

Matić, S., Stanić, S., Bogojević, D., Solujić, S., Mladenović, M., Stanković, N., Mihailović, V., Katanić, J.,& Mihailović, M.. (2013). Chemical composition, antioxidant and antigenotoxic activities of Cotinus coggygria stem extract. in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia
Belgrade: Serbian Plant Physiology Society., 90-91.
https://hdl.handle.net/21.15107/rcub_ibiss_6141

Matić S, Stanić S, Bogojević D, Solujić S, Mladenović M, Stanković N, Mihailović V, Katanić J, Mihailović M. Chemical composition, antioxidant and antigenotoxic activities of Cotinus coggygria stem extract. in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia. 2013;:90-91.
https://hdl.handle.net/21.15107/rcub_ibiss_6141 .

Matić, Sanja, Stanić, Snežana, Bogojević, Desanka, Solujić, Slavica, Mladenović, Milan, Stanković, Nevena, Mihailović, Vladimir, Katanić, Jelena, Mihailović, Mirjana, "Chemical composition, antioxidant and antigenotoxic activities of Cotinus coggygria stem extract" in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia (2013):90-91,
https://hdl.handle.net/21.15107/rcub_ibiss_6141 .