TY  - CONF
AU  - Vujošević, Mladen
AU  - Rajičić, Marija
AU  - Budinski, Ivana
AU  - Bajić, Branka
AU  - Adnađević, Tanja
AU  - Blagojević, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6429
AB  - B chromosomes (Bs) are uncommon in mammals, featuring less than 2% of species, but six out of 22 species possess them in the genus Apodemus. In Serbia, over 40 populations of yellow-necked mice
(Apodemus flavicollis) with varying habitat quality were studied. The frequency of B carriers (range: 0.09 to 0.67) increased with elevation, correlated with sub-zero days, and inversely with average temperature. B carrier frequency remained stable over eight years despite population density fluctuations, while seasonal variations linked to population size were observed. Overcrowding stress reduced B carriers among pre-reproductive individuals but enhanced survival in suboptimal conditions.
Phenotypic traits and B frequency were correlated, influencing cranial morphometric development. B carriers showed distinct developmental pathways for cranial traits, suggesting environment-specific benefits. Bs’ presence did not affect carrier fecundity or fertility and was evenly distributed across age groups. B chromosomes (up to five) displayed common structures across populations in Serbia and Eastern Europe, possibly originating from sex chromosome pericentromeric regions. B-specific chromatin spatially resembled pericentromeric sex chromosomes, suggesting a similar mechanism to bypass meiotic
checkpoints.
Current data support a heterotic model in A. flavicollis. Bs likely contribute to species adaptability by increasing genetic variability, potentially expanding their distribution.
PB  - Springer Nature
C3  - Meeting Abstracts from the 5th B Chromosome Conference; 2023 Oct 14-17; Petnica, Serbia
T1  - B chromosomes in populations of Apodemus flavicollis – never ending story
DO  - 10.1186/s12919-023-00284-9
SP  - 33
ER  - 

@conference{
author = "Vujošević, Mladen and Rajičić, Marija and Budinski, Ivana and Bajić, Branka and Adnađević, Tanja and Blagojević, Jelena",
year = "2023",
abstract = "B chromosomes (Bs) are uncommon in mammals, featuring less than 2% of species, but six out of 22 species possess them in the genus Apodemus. In Serbia, over 40 populations of yellow-necked mice
(Apodemus flavicollis) with varying habitat quality were studied. The frequency of B carriers (range: 0.09 to 0.67) increased with elevation, correlated with sub-zero days, and inversely with average temperature. B carrier frequency remained stable over eight years despite population density fluctuations, while seasonal variations linked to population size were observed. Overcrowding stress reduced B carriers among pre-reproductive individuals but enhanced survival in suboptimal conditions.
Phenotypic traits and B frequency were correlated, influencing cranial morphometric development. B carriers showed distinct developmental pathways for cranial traits, suggesting environment-specific benefits. Bs’ presence did not affect carrier fecundity or fertility and was evenly distributed across age groups. B chromosomes (up to five) displayed common structures across populations in Serbia and Eastern Europe, possibly originating from sex chromosome pericentromeric regions. B-specific chromatin spatially resembled pericentromeric sex chromosomes, suggesting a similar mechanism to bypass meiotic
checkpoints.
Current data support a heterotic model in A. flavicollis. Bs likely contribute to species adaptability by increasing genetic variability, potentially expanding their distribution.",
publisher = "Springer Nature",
journal = "Meeting Abstracts from the 5th B Chromosome Conference; 2023 Oct 14-17; Petnica, Serbia",
title = "B chromosomes in populations of Apodemus flavicollis – never ending story",
doi = "10.1186/s12919-023-00284-9",
pages = "33"
}

Vujošević, M., Rajičić, M., Budinski, I., Bajić, B., Adnađević, T.,& Blagojević, J.. (2023). B chromosomes in populations of Apodemus flavicollis – never ending story. in Meeting Abstracts from the 5th B Chromosome Conference; 2023 Oct 14-17; Petnica, Serbia
Springer Nature., 33.
https://doi.org/10.1186/s12919-023-00284-9

Vujošević M, Rajičić M, Budinski I, Bajić B, Adnađević T, Blagojević J. B chromosomes in populations of Apodemus flavicollis – never ending story. in Meeting Abstracts from the 5th B Chromosome Conference; 2023 Oct 14-17; Petnica, Serbia. 2023;:33.
doi:10.1186/s12919-023-00284-9 .

Vujošević, Mladen, Rajičić, Marija, Budinski, Ivana, Bajić, Branka, Adnađević, Tanja, Blagojević, Jelena, "B chromosomes in populations of Apodemus flavicollis – never ending story" in Meeting Abstracts from the 5th B Chromosome Conference; 2023 Oct 14-17; Petnica, Serbia (2023):33,
https://doi.org/10.1186/s12919-023-00284-9 . .

TY  - THES
AU  - Bajić, Branka
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5881
AB  - Studija predstavlja prvo istraživanje genetičkog diverziteta mitohondrijskih gena beskrilnih muva i grinja, ektoparazita dve vrste slepih miševa, u Srbiji. Analizirani su sastav vrsta, specifičnost prema domaćinu i struktura populacija, te kvantitativni parametri parazitiranosti. Muve su testirane na prisustvo krvnog parazita Polychromophilus sp. čiji je genetički diverzitet takođe analiziran. Analizirani su genetički diverzitet i struktura populacija jedne vrste domaćina, M. schreibersii, koji su potom poređeni sa parametrima ektoparazita nađenih na njemu. Uzorci su prikupljeni sa 9 lokaliteta u Srbiji i Bosni i Hercegovini, sa ukupno 241 jedinke domaćina (Miniopterus schreibersii 168, Rhinolophus ferrumequinum 73). Prikupljeno je 369 jedinki beskrilnih muva (4 vrste) i 564 jedinki grinja (2 vrste), sa izuzetno izraženom specifičnošću prema primarnom domaćinu i bez ijednog zabeleženog slučaja unakrsne infekcije, uprkos činjenici da su domaćinske vrste povremeno delile skloništa. Domaćinska vrsta koja formira veće i gušće kolonije i migrira na veće distance, M. schreibersii, imala je veći intenzitet infekcije i diverzitet vrsta beskrilnih muva, a muve prikupljene sa nje imale su veći genetički diverzitet od muva prikupljenih sa drugog, manje mobilnog domaćina, R. ferrumequinum, u skladu sa očekivanjama. Parametri genetičkog diverziteta domaćina M. schreibersii i dveju ektoparazitskih vrsta, muve Nycteribia schmidlii i grinje Spinturnix psi nisu bili značajno korelisani. Infekcija parazitom Polychromophilus melanipherus detektovana je kod 33 beskrilne muve, od čega je samo jedna bila poreklom sa domaćina R. ferrumequinum - prvi takav slučaj zabeležen do sad. Potvrđen je značaj upotrebe ektoparazita koji se hrane krvlju u neinvazivnom testiranju populacija slepih miševa na prisustvo infekcija.
AB  - This study represents the first investigation of genetic diversity of mitochondrial genes of bat flies and mites, ectoparasites of two bat species, in Serbia. Species composition, host specificity, population structure and quantitative parameters of parasitism were analyzed. The flies were tested for the presence of the blood parasite Polychromophilus sp., whose genetic diversity was also analyzed. The genetic diversity and population structure of one host species, Miniopterus schreibersii, were analyzed and then compared with those of its ectoparasites. Samples were collected from 9 sites in Serbia and Bosnia and Herzegovina from a total of 241 host individuals (M. schreibersii 168, Rhinolophus ferrumequinum 73). 369 individuals of bat flies (4 species) and 564 individuals of mites (2 species) were collected, with a marked specificity for the primary host and without a single case of cross-infection recorded, despite the fact that the host species occasionally shared roosts. A host species that forms larger and denser colonies and migrates over longer distances, M. schreibersii, had a higher intensity of infection and species diversity of bat flies, and the flies collected from it had greater genetic diversity than those collected from the other, less mobile host R. ferrumequinum, as expected. The genetic diversity parameters of the host M. schreibersii and two ectoparasitic species, the bat fly Nycteribia schmidlii and the mite Spinturnix psi, were not significantly correlated. Infection with the parasite Polychromophilus melanipherus was detected in 33 bat flies, only one of which originated from the host R. ferrumequinum - the first such case recorded so far. The importance of using blood-feeding ectoparasites in non-invasive surveys of bat populations for the presence of infections was confirmed.
PB  - Belgrade: University of Belgrade, Faculty of Biology
T1  - Genetički diverzitet i struktura populacija beskrilnih muva familije Nycteribiidae i grinja familije Spinturnicidae, ektoparazita dve litofilne vrste slepih miševa u Srbiji
T1  - Genetic diversity and population structure of bat flies of the family Nycteribiidae and mites of the family Spinturnicidae, ectoparasites of the two cavernicolous bat species in Serbia
SP  - 1
EP  - 125
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5881
ER  - 

@phdthesis{
author = "Bajić, Branka",
year = "2023",
abstract = "Studija predstavlja prvo istraživanje genetičkog diverziteta mitohondrijskih gena beskrilnih muva i grinja, ektoparazita dve vrste slepih miševa, u Srbiji. Analizirani su sastav vrsta, specifičnost prema domaćinu i struktura populacija, te kvantitativni parametri parazitiranosti. Muve su testirane na prisustvo krvnog parazita Polychromophilus sp. čiji je genetički diverzitet takođe analiziran. Analizirani su genetički diverzitet i struktura populacija jedne vrste domaćina, M. schreibersii, koji su potom poređeni sa parametrima ektoparazita nađenih na njemu. Uzorci su prikupljeni sa 9 lokaliteta u Srbiji i Bosni i Hercegovini, sa ukupno 241 jedinke domaćina (Miniopterus schreibersii 168, Rhinolophus ferrumequinum 73). Prikupljeno je 369 jedinki beskrilnih muva (4 vrste) i 564 jedinki grinja (2 vrste), sa izuzetno izraženom specifičnošću prema primarnom domaćinu i bez ijednog zabeleženog slučaja unakrsne infekcije, uprkos činjenici da su domaćinske vrste povremeno delile skloništa. Domaćinska vrsta koja formira veće i gušće kolonije i migrira na veće distance, M. schreibersii, imala je veći intenzitet infekcije i diverzitet vrsta beskrilnih muva, a muve prikupljene sa nje imale su veći genetički diverzitet od muva prikupljenih sa drugog, manje mobilnog domaćina, R. ferrumequinum, u skladu sa očekivanjama. Parametri genetičkog diverziteta domaćina M. schreibersii i dveju ektoparazitskih vrsta, muve Nycteribia schmidlii i grinje Spinturnix psi nisu bili značajno korelisani. Infekcija parazitom Polychromophilus melanipherus detektovana je kod 33 beskrilne muve, od čega je samo jedna bila poreklom sa domaćina R. ferrumequinum - prvi takav slučaj zabeležen do sad. Potvrđen je značaj upotrebe ektoparazita koji se hrane krvlju u neinvazivnom testiranju populacija slepih miševa na prisustvo infekcija., This study represents the first investigation of genetic diversity of mitochondrial genes of bat flies and mites, ectoparasites of two bat species, in Serbia. Species composition, host specificity, population structure and quantitative parameters of parasitism were analyzed. The flies were tested for the presence of the blood parasite Polychromophilus sp., whose genetic diversity was also analyzed. The genetic diversity and population structure of one host species, Miniopterus schreibersii, were analyzed and then compared with those of its ectoparasites. Samples were collected from 9 sites in Serbia and Bosnia and Herzegovina from a total of 241 host individuals (M. schreibersii 168, Rhinolophus ferrumequinum 73). 369 individuals of bat flies (4 species) and 564 individuals of mites (2 species) were collected, with a marked specificity for the primary host and without a single case of cross-infection recorded, despite the fact that the host species occasionally shared roosts. A host species that forms larger and denser colonies and migrates over longer distances, M. schreibersii, had a higher intensity of infection and species diversity of bat flies, and the flies collected from it had greater genetic diversity than those collected from the other, less mobile host R. ferrumequinum, as expected. The genetic diversity parameters of the host M. schreibersii and two ectoparasitic species, the bat fly Nycteribia schmidlii and the mite Spinturnix psi, were not significantly correlated. Infection with the parasite Polychromophilus melanipherus was detected in 33 bat flies, only one of which originated from the host R. ferrumequinum - the first such case recorded so far. The importance of using blood-feeding ectoparasites in non-invasive surveys of bat populations for the presence of infections was confirmed.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
title = "Genetički diverzitet i struktura populacija beskrilnih muva familije Nycteribiidae i grinja familije Spinturnicidae, ektoparazita dve litofilne vrste slepih miševa u Srbiji, Genetic diversity and population structure of bat flies of the family Nycteribiidae and mites of the family Spinturnicidae, ectoparasites of the two cavernicolous bat species in Serbia",
pages = "1-125",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5881"
}

Bajić, B.. (2023). Genetički diverzitet i struktura populacija beskrilnih muva familije Nycteribiidae i grinja familije Spinturnicidae, ektoparazita dve litofilne vrste slepih miševa u Srbiji. 
Belgrade: University of Belgrade, Faculty of Biology., 1-125.
https://hdl.handle.net/21.15107/rcub_ibiss_5881

Bajić B. Genetički diverzitet i struktura populacija beskrilnih muva familije Nycteribiidae i grinja familije Spinturnicidae, ektoparazita dve litofilne vrste slepih miševa u Srbiji. 2023;:1-125.
https://hdl.handle.net/21.15107/rcub_ibiss_5881 .

Bajić, Branka, "Genetički diverzitet i struktura populacija beskrilnih muva familije Nycteribiidae i grinja familije Spinturnicidae, ektoparazita dve litofilne vrste slepih miševa u Srbiji" (2023):1-125,
https://hdl.handle.net/21.15107/rcub_ibiss_5881 .

TY  - JOUR
AU  - Jojić, Vida
AU  - Čabrilo, Borislav
AU  - Bjelić-Čabrilo, Olivera
AU  - Jovanović, Vladimir
AU  - Budinski, Ivana
AU  - Vujošević, Mladen
AU  - Blagojević, Jelena
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4645
AB  - Background:  Mammalian mandible and cranium are well‑established model systems for studying canalization 
and developmental stability (DS) as two elements of developmental homeostasis. Nematode infections are usually 
acquired in early life and increase in intensity with age, while canalization and DS of rodent skulls could vary through 
late postnatal ontogeny. We aimed to estimate magnitudes and describe patterns of mandibular and cranial canaliza‑
tion and DS related to age and parasite intensity (diversity) in adult yellow‑necked mice (Apodemus favicollis).
Results:  We found the absence of age‑related changes in the levels of canalization for mandibular and cranial size 
and DS for mandibular size. However, individual measures of mandibular and cranial shape variance increased, while 
individual measures of mandibular shape fuctuating asymmetry (FA) decreased with age. We detected mandibular 
and cranial shape changes during postnatal ontogeny, but revealed no age‑related dynamics of their covariance 
structure among and within individuals. Categories regarding parasitism difered in the level of canalization for cranial 
size and the level of DS for cranial shape. We observed diferences in age‑related dynamics of the level of canalization 
between non‑parasitized and parasitized animals, as well as between yellow‑necked mice parasitized by diferent 
number of nematode species. Likewise, individual measures of mandibular and cranial shape FA decreased with age 
for the mandible in the less parasitized category and increased for the cranium in the most parasitized category.
Conclusions:  Our age‑related results partly agree with previous fndings. However, no rodent study so far has 
explored age‑related changes in the magnitude of FA for mandibular size or mandibular and cranial FA covariance 
structure. This is the frst study dealing with the nematode parasitism‑related canalization and DS in rodents. We 
showed that nematode parasitism does not afect mandibular and cranial shape variation and covariance structure 
among and within individuals. However, parasite intensity (diversity) is related to ontogenetic dynamics of the levels 
of canalization and DS. Overall, additional studies on animals from natural populations are required before drawing 
some general conclusions.
PB  - BioMed Central Ltd. Part of Springer Nature.
T2  - Frontiers in Zoology
T1  - Canalization and developmental stability  of the yellow-necked mouse (Apodemus  favicollis) mandible and cranium related to age  and nematode parasitism
VL  - 18
DO  - 10.1186/s12983-021-00439-4
SP  - 55
ER  - 

@article{
author = "Jojić, Vida and Čabrilo, Borislav and Bjelić-Čabrilo, Olivera and Jovanović, Vladimir and Budinski, Ivana and Vujošević, Mladen and Blagojević, Jelena",
year = "2021",
abstract = "Background:  Mammalian mandible and cranium are well‑established model systems for studying canalization 
and developmental stability (DS) as two elements of developmental homeostasis. Nematode infections are usually 
acquired in early life and increase in intensity with age, while canalization and DS of rodent skulls could vary through 
late postnatal ontogeny. We aimed to estimate magnitudes and describe patterns of mandibular and cranial canaliza‑
tion and DS related to age and parasite intensity (diversity) in adult yellow‑necked mice (Apodemus favicollis).
Results:  We found the absence of age‑related changes in the levels of canalization for mandibular and cranial size 
and DS for mandibular size. However, individual measures of mandibular and cranial shape variance increased, while 
individual measures of mandibular shape fuctuating asymmetry (FA) decreased with age. We detected mandibular 
and cranial shape changes during postnatal ontogeny, but revealed no age‑related dynamics of their covariance 
structure among and within individuals. Categories regarding parasitism difered in the level of canalization for cranial 
size and the level of DS for cranial shape. We observed diferences in age‑related dynamics of the level of canalization 
between non‑parasitized and parasitized animals, as well as between yellow‑necked mice parasitized by diferent 
number of nematode species. Likewise, individual measures of mandibular and cranial shape FA decreased with age 
for the mandible in the less parasitized category and increased for the cranium in the most parasitized category.
Conclusions:  Our age‑related results partly agree with previous fndings. However, no rodent study so far has 
explored age‑related changes in the magnitude of FA for mandibular size or mandibular and cranial FA covariance 
structure. This is the frst study dealing with the nematode parasitism‑related canalization and DS in rodents. We 
showed that nematode parasitism does not afect mandibular and cranial shape variation and covariance structure 
among and within individuals. However, parasite intensity (diversity) is related to ontogenetic dynamics of the levels 
of canalization and DS. Overall, additional studies on animals from natural populations are required before drawing 
some general conclusions.",
publisher = "BioMed Central Ltd. Part of Springer Nature.",
journal = "Frontiers in Zoology",
title = "Canalization and developmental stability  of the yellow-necked mouse (Apodemus  favicollis) mandible and cranium related to age  and nematode parasitism",
volume = "18",
doi = "10.1186/s12983-021-00439-4",
pages = "55"
}

Jojić, V., Čabrilo, B., Bjelić-Čabrilo, O., Jovanović, V., Budinski, I., Vujošević, M.,& Blagojević, J.. (2021). Canalization and developmental stability  of the yellow-necked mouse (Apodemus  favicollis) mandible and cranium related to age  and nematode parasitism. in Frontiers in Zoology
BioMed Central Ltd. Part of Springer Nature.., 18, 55.
https://doi.org/10.1186/s12983-021-00439-4

Jojić V, Čabrilo B, Bjelić-Čabrilo O, Jovanović V, Budinski I, Vujošević M, Blagojević J. Canalization and developmental stability  of the yellow-necked mouse (Apodemus  favicollis) mandible and cranium related to age  and nematode parasitism. in Frontiers in Zoology. 2021;18:55.
doi:10.1186/s12983-021-00439-4 .

Jojić, Vida, Čabrilo, Borislav, Bjelić-Čabrilo, Olivera, Jovanović, Vladimir, Budinski, Ivana, Vujošević, Mladen, Blagojević, Jelena, "Canalization and developmental stability  of the yellow-necked mouse (Apodemus  favicollis) mandible and cranium related to age  and nematode parasitism" in Frontiers in Zoology, 18 (2021):55,
https://doi.org/10.1186/s12983-021-00439-4 . .

TY  - CONF
AU  - Budinski, Ivana
AU  - Bajić, Branka
AU  - Karapandža, Branko
AU  - Bogosavljević, Jelena
AU  - Paunović, Milan
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4260
AB  - Canetova pećina se nalazi u klisuri reke Zamne između sela Plavna i Štubik, na oko 260 m n.v. Prvi podaci o fauni slepih miševa Canetove pećine datiraju s kraja 80-ih godina XX veka. Od tog perioda do danas je pećina posećivana u više navrata i podaci o prisutnim slepim miševima su beleženi na osnovu vizualnih opservacija i ultrazvučne detekcije. Određen broj jedinki je bio uhvaćen pomoću ručne mreže unutar skloništa, odnosno vertikalne mreže postavljene na pećinskom ulazu. Ukupno je zabeleženo prisustvo sedam vrsta slepih miševa: Rhinolophus ferrumequinum, R. euryale, R. blasii, R. mehelyi, R. hipposideros, Miniopterus schreibersii i Myotis capaccinii. U tom periodu je markirano 130 jedinki slepih miševa krilnim markerima Centra za markiranje životinja pri Prirodnjačkom muzeju u Beogradu, od kojih su 2 jedinke bile ponovno hvatane. Suprotno očekivanjima, šumske vrste slepih miševa ne koriste ovo sklonište za parenje i rojenje pred hibernaciju. Ova pećina predstavlja značajno kopulatorno sklonište vrsta Rhinolophus ferrumequinum, Miniopterus schreibersii i Myotis capaciinii, koje su zabeležene na hibernaciji u Dudićevoj pećini koja se nalazi u neposrednoj blizini. U Canetovoj pećini se nalazi jedna od najvećih hibernacijskih kolonija srednjih potkovičara u Srbiji, u kojoj su najbrojniji predstavnici sredozemnog potkovičara Rhinolophus euryale. U februaru 2019. godine je prilikom zimskog monitoringa prebrojano oko 800 jedinki srednjih potkovičara na hibernaciji. Canetova pećina predstavlja jedno od svega četiri skloništa u Srbiji na kojima je zabeležen tamnooki potkovičar Rhinolophus mehelyi, a ujedno i jedan od dva lokaliteta u Srbiji na kojima je prisutno svih pet vrsta potkovičara. U pećini postoje ogromne naslage guana koje svedoče o dugoročnoj upotrebi skloništa od strane slepih miševa, kao i veličini njihovih kolonija. Canetova pećina ima izuzetan značaj za slepe miševe u tranzitornom periodu kao i u periodu kopulacije i hibernacije, dok nema podataka o fauni slepih miševa u ovom skloništu tokom leta, zbog čega je neophodno nastaviti istraživanja.
PB  - Beograd: Akademski speleološko – alpinistički klub (ASAK)
T1  - Fauna slepih miševa Canetove pećine
SP  - 17
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4260
ER  - 

@conference{
author = "Budinski, Ivana and Bajić, Branka and Karapandža, Branko and Bogosavljević, Jelena and Paunović, Milan",
year = "2019",
abstract = "Canetova pećina se nalazi u klisuri reke Zamne između sela Plavna i Štubik, na oko 260 m n.v. Prvi podaci o fauni slepih miševa Canetove pećine datiraju s kraja 80-ih godina XX veka. Od tog perioda do danas je pećina posećivana u više navrata i podaci o prisutnim slepim miševima su beleženi na osnovu vizualnih opservacija i ultrazvučne detekcije. Određen broj jedinki je bio uhvaćen pomoću ručne mreže unutar skloništa, odnosno vertikalne mreže postavljene na pećinskom ulazu. Ukupno je zabeleženo prisustvo sedam vrsta slepih miševa: Rhinolophus ferrumequinum, R. euryale, R. blasii, R. mehelyi, R. hipposideros, Miniopterus schreibersii i Myotis capaccinii. U tom periodu je markirano 130 jedinki slepih miševa krilnim markerima Centra za markiranje životinja pri Prirodnjačkom muzeju u Beogradu, od kojih su 2 jedinke bile ponovno hvatane. Suprotno očekivanjima, šumske vrste slepih miševa ne koriste ovo sklonište za parenje i rojenje pred hibernaciju. Ova pećina predstavlja značajno kopulatorno sklonište vrsta Rhinolophus ferrumequinum, Miniopterus schreibersii i Myotis capaciinii, koje su zabeležene na hibernaciji u Dudićevoj pećini koja se nalazi u neposrednoj blizini. U Canetovoj pećini se nalazi jedna od najvećih hibernacijskih kolonija srednjih potkovičara u Srbiji, u kojoj su najbrojniji predstavnici sredozemnog potkovičara Rhinolophus euryale. U februaru 2019. godine je prilikom zimskog monitoringa prebrojano oko 800 jedinki srednjih potkovičara na hibernaciji. Canetova pećina predstavlja jedno od svega četiri skloništa u Srbiji na kojima je zabeležen tamnooki potkovičar Rhinolophus mehelyi, a ujedno i jedan od dva lokaliteta u Srbiji na kojima je prisutno svih pet vrsta potkovičara. U pećini postoje ogromne naslage guana koje svedoče o dugoročnoj upotrebi skloništa od strane slepih miševa, kao i veličini njihovih kolonija. Canetova pećina ima izuzetan značaj za slepe miševe u tranzitornom periodu kao i u periodu kopulacije i hibernacije, dok nema podataka o fauni slepih miševa u ovom skloništu tokom leta, zbog čega je neophodno nastaviti istraživanja.",
publisher = "Beograd: Akademski speleološko – alpinistički klub (ASAK)",
title = "Fauna slepih miševa Canetove pećine",
pages = "17",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4260"
}

Budinski, I., Bajić, B., Karapandža, B., Bogosavljević, J.,& Paunović, M.. (2019). Fauna slepih miševa Canetove pećine. 
Beograd: Akademski speleološko – alpinistički klub (ASAK)., 17.
https://hdl.handle.net/21.15107/rcub_ibiss_4260

Budinski I, Bajić B, Karapandža B, Bogosavljević J, Paunović M. Fauna slepih miševa Canetove pećine. 2019;:17.
https://hdl.handle.net/21.15107/rcub_ibiss_4260 .

Budinski, Ivana, Bajić, Branka, Karapandža, Branko, Bogosavljević, Jelena, Paunović, Milan, "Fauna slepih miševa Canetove pećine" (2019):17,
https://hdl.handle.net/21.15107/rcub_ibiss_4260 .

TY  - CONF
AU  - Bajić, Branka
AU  - Bogosavljević, Jelena
AU  - Paunović, Milan
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4265
AB  - European bat species feed exclusively on insects, and due to lack of prey during the cold
months of the year, many species spend the winter hibernating within the underground roosts. During the regular monitoring scheme, from February 15th to February 17th, 2019, ten speleological objects in Eastern and Western Serbia were checked for the presence of bats, resulting in records of over 29 000 individuals. There were 11 recorded species: Rhinolophus ferrumequinum, R. euryale, R. blasii, R. hipposideros, Miniopterus schreibersii, Myotis myotis/blythii, M. capaccinii, Eptesicus serotinus, Nyctalus noctula, Plecotus auritus and Pipistrellus sp. The monitoring activities included entering the objects and performing a detailed search of all areas, identifying species without capturing or disturbing them, counting the bats on-site or from photographs (spotcounting method) or estimate of the number of individuals by using block method in case of
extremely large groups when it was impossible to directly count bats. Among the surveyed caves in Eastern Serbia (Ravanička Pećina, Toplik, Vernjikica, Lazareva Pećina, Canetova Pećina, Dudićeva Pećina and Gradašnička Pećina), the most outstandingcave was Vernjikica in the vicinity of village Zlot near the city Bor. It stands out both by its size and by the number of hibernating bats (around
25 000 individuals), while the dominant species was Schreiber's Bent-winged Bat Miniopterus schreibersii. Monitoring was also performed in three caves in Western Serbia (Ćebića Pećina, Tmuša and Petnička Pećina), where Ćebića Pećina was the most impotant to mention, both because the number of recorded species and number of recoveries of previously marked individuals. Regular monitoring activities in caves during the hibernation season is important for keeping track of bat population size and structure, as well as for identifying key roosts that need to be protected.
PB  - Belgrade: Student Speleological and Alpinistic club (ASAK)
C3  - 9th Symposium on karst protection; 2019 Nov 1-3; Beograd, Serbia
T1  - Monitoring of bat hibernation colonies in ten caves in Serbia in February 2019
SP  - 18
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4265
ER  - 

@conference{
author = "Bajić, Branka and Bogosavljević, Jelena and Paunović, Milan",
year = "2019",
abstract = "European bat species feed exclusively on insects, and due to lack of prey during the cold
months of the year, many species spend the winter hibernating within the underground roosts. During the regular monitoring scheme, from February 15th to February 17th, 2019, ten speleological objects in Eastern and Western Serbia were checked for the presence of bats, resulting in records of over 29 000 individuals. There were 11 recorded species: Rhinolophus ferrumequinum, R. euryale, R. blasii, R. hipposideros, Miniopterus schreibersii, Myotis myotis/blythii, M. capaccinii, Eptesicus serotinus, Nyctalus noctula, Plecotus auritus and Pipistrellus sp. The monitoring activities included entering the objects and performing a detailed search of all areas, identifying species without capturing or disturbing them, counting the bats on-site or from photographs (spotcounting method) or estimate of the number of individuals by using block method in case of
extremely large groups when it was impossible to directly count bats. Among the surveyed caves in Eastern Serbia (Ravanička Pećina, Toplik, Vernjikica, Lazareva Pećina, Canetova Pećina, Dudićeva Pećina and Gradašnička Pećina), the most outstandingcave was Vernjikica in the vicinity of village Zlot near the city Bor. It stands out both by its size and by the number of hibernating bats (around
25 000 individuals), while the dominant species was Schreiber's Bent-winged Bat Miniopterus schreibersii. Monitoring was also performed in three caves in Western Serbia (Ćebića Pećina, Tmuša and Petnička Pećina), where Ćebića Pećina was the most impotant to mention, both because the number of recorded species and number of recoveries of previously marked individuals. Regular monitoring activities in caves during the hibernation season is important for keeping track of bat population size and structure, as well as for identifying key roosts that need to be protected.",
publisher = "Belgrade: Student Speleological and Alpinistic club (ASAK)",
journal = "9th Symposium on karst protection; 2019 Nov 1-3; Beograd, Serbia",
title = "Monitoring of bat hibernation colonies in ten caves in Serbia in February 2019",
pages = "18",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4265"
}

Bajić, B., Bogosavljević, J.,& Paunović, M.. (2019). Monitoring of bat hibernation colonies in ten caves in Serbia in February 2019. in 9th Symposium on karst protection; 2019 Nov 1-3; Beograd, Serbia
Belgrade: Student Speleological and Alpinistic club (ASAK)., 18.
https://hdl.handle.net/21.15107/rcub_ibiss_4265

Bajić B, Bogosavljević J, Paunović M. Monitoring of bat hibernation colonies in ten caves in Serbia in February 2019. in 9th Symposium on karst protection; 2019 Nov 1-3; Beograd, Serbia. 2019;:18.
https://hdl.handle.net/21.15107/rcub_ibiss_4265 .

Bajić, Branka, Bogosavljević, Jelena, Paunović, Milan, "Monitoring of bat hibernation colonies in ten caves in Serbia in February 2019" in 9th Symposium on karst protection; 2019 Nov 1-3; Beograd, Serbia (2019):18,
https://hdl.handle.net/21.15107/rcub_ibiss_4265 .

TY  - JOUR
AU  - Budinski, Ivana
AU  - Jovanović, Vladimir
AU  - Bajić, Branka
AU  - Blagojević, Jelena
AU  - Rajičić, Marija
AU  - Paunović, Milan
AU  - Presetnik, Primož
AU  - Vujošević, Mladen
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3543
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/4330
AB  - The Balkan Peninsula is identified as one of the major glacial refugia in Europe during the
Pleistocene, and it has served as a genetic source for post-glacial recolonization for many temperate
species. The aim of this study was to investigate the genetic diversity and phylogeographic patterns of the
Mediterranean horseshoe bat, Rhinolophus euryale Blasius 1853, on the Balkan Peninsula. We also
analyzed its demographic history and tested the hypothesis that this region was a glacial refugium for this
species. We collected 82 samples from 20 localities in the Balkans and Italy and sequenced the
mitochondrial D-loop region. Our results revealed low nucleotide but high haplotype diversity, with 20
out of 24 haplotypes reported for the first time. All Balkan and Italian samples belonged to a single
genetic clade in the phylogenetic reconstruction, where they clustered together with previously published
samples from Turkey, southern France and North Africa. The haplotype network had a star-like pattern
that is indicative of recent population expansion. Both mismatch distribution and shallow genetic
differentiation also supported the scenario of a sudden demographic expansion. We estimated that
expansion within this lineage commenced in the Late Pleistocene. We suggest that the Balkan Peninsula
was a glacial refugium for R. euryale.
PB  - Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Mitochondrial phylogeography of the Mediterranean horseshoe bat on the Balkan Peninsula
IS  - 4
VL  - 71
DO  - 10.2298/ABS190529059B
SP  - 767
EP  - 774
ER  - 

@article{
author = "Budinski, Ivana and Jovanović, Vladimir and Bajić, Branka and Blagojević, Jelena and Rajičić, Marija and Paunović, Milan and Presetnik, Primož and Vujošević, Mladen",
year = "2019",
abstract = "The Balkan Peninsula is identified as one of the major glacial refugia in Europe during the
Pleistocene, and it has served as a genetic source for post-glacial recolonization for many temperate
species. The aim of this study was to investigate the genetic diversity and phylogeographic patterns of the
Mediterranean horseshoe bat, Rhinolophus euryale Blasius 1853, on the Balkan Peninsula. We also
analyzed its demographic history and tested the hypothesis that this region was a glacial refugium for this
species. We collected 82 samples from 20 localities in the Balkans and Italy and sequenced the
mitochondrial D-loop region. Our results revealed low nucleotide but high haplotype diversity, with 20
out of 24 haplotypes reported for the first time. All Balkan and Italian samples belonged to a single
genetic clade in the phylogenetic reconstruction, where they clustered together with previously published
samples from Turkey, southern France and North Africa. The haplotype network had a star-like pattern
that is indicative of recent population expansion. Both mismatch distribution and shallow genetic
differentiation also supported the scenario of a sudden demographic expansion. We estimated that
expansion within this lineage commenced in the Late Pleistocene. We suggest that the Balkan Peninsula
was a glacial refugium for R. euryale.",
publisher = "Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Mitochondrial phylogeography of the Mediterranean horseshoe bat on the Balkan Peninsula",
number = "4",
volume = "71",
doi = "10.2298/ABS190529059B",
pages = "767-774"
}

Budinski, I., Jovanović, V., Bajić, B., Blagojević, J., Rajičić, M., Paunović, M., Presetnik, P.,& Vujošević, M.. (2019). Mitochondrial phylogeography of the Mediterranean horseshoe bat on the Balkan Peninsula. in Archives of Biological Sciences
Serbian Biological Society., 71(4), 767-774.
https://doi.org/10.2298/ABS190529059B

Budinski I, Jovanović V, Bajić B, Blagojević J, Rajičić M, Paunović M, Presetnik P, Vujošević M. Mitochondrial phylogeography of the Mediterranean horseshoe bat on the Balkan Peninsula. in Archives of Biological Sciences. 2019;71(4):767-774.
doi:10.2298/ABS190529059B .

Budinski, Ivana, Jovanović, Vladimir, Bajić, Branka, Blagojević, Jelena, Rajičić, Marija, Paunović, Milan, Presetnik, Primož, Vujošević, Mladen, "Mitochondrial phylogeography of the Mediterranean horseshoe bat on the Balkan Peninsula" in Archives of Biological Sciences, 71, no. 4 (2019):767-774,
https://doi.org/10.2298/ABS190529059B . .

TY  - JOUR
AU  - Budinski, Ivana
AU  - Blagojević, Jelena
AU  - Jovanović, Vladimir
AU  - Bajić, Branka
AU  - Adnađević, Tanja
AU  - Paunović, Milan
AU  - Vujošević, Mladen
PY  - 2019
UR  - http://dx.plos.org/10.1371/journal.pone.0210321
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC6353099
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3262
AB  - Migratory behaviour, sociality and roost selection have a great impact on the population structure of one species. Many bat species live in groups, and movements between summer and hibernation sites are common in temperate bats. The Mediterranean horseshoe bat Rhinolophus euryale is a cave-dwelling species that exhibits roost philopatry and undertakes seasonal movements which are usually shorter than 50 km. Its distribution in Serbia is restricted to karstic areas in western and eastern parts of the country, with a lack of known roosts between them. In this study, microsatellite markers were used to evaluate genetic variation in this species in the Central Balkans. Specifically, spatial genetic structuring between geographic regions and relatedness within different colony types were assessed. All analysed loci were polymorphic, and there was no significant inbreeding coefficient recorded. A moderate degree of genetic differentiation among the sampled colonies was found, and significant isolation by distance was recorded. Our results revealed that populations show a tendency to segregate into three clusters. Unexpectedly, populations from Montenegro and Eastern Serbia tended to group into one cluster, while populations from Western Serbia and Slovenia represented second and third cluster, respectively. The majority of variance was partitioned within colonies, and only a small but significant portion among clusters. Average relatedness within colony members was close to zero, did not differ significantly between the different colony types, and kinship is unlikely to be a major grouping mechanism in this species.
T2  - PloS One
T1  - Population genetic structure of the Mediterranean horseshoe bat Rhinolophus euryale in the central Balkans.
IS  - 1
VL  - 14
DO  - 10.1371/journal.pone.0210321
SP  - e0210321
ER  - 

@article{
author = "Budinski, Ivana and Blagojević, Jelena and Jovanović, Vladimir and Bajić, Branka and Adnađević, Tanja and Paunović, Milan and Vujošević, Mladen",
year = "2019",
abstract = "Migratory behaviour, sociality and roost selection have a great impact on the population structure of one species. Many bat species live in groups, and movements between summer and hibernation sites are common in temperate bats. The Mediterranean horseshoe bat Rhinolophus euryale is a cave-dwelling species that exhibits roost philopatry and undertakes seasonal movements which are usually shorter than 50 km. Its distribution in Serbia is restricted to karstic areas in western and eastern parts of the country, with a lack of known roosts between them. In this study, microsatellite markers were used to evaluate genetic variation in this species in the Central Balkans. Specifically, spatial genetic structuring between geographic regions and relatedness within different colony types were assessed. All analysed loci were polymorphic, and there was no significant inbreeding coefficient recorded. A moderate degree of genetic differentiation among the sampled colonies was found, and significant isolation by distance was recorded. Our results revealed that populations show a tendency to segregate into three clusters. Unexpectedly, populations from Montenegro and Eastern Serbia tended to group into one cluster, while populations from Western Serbia and Slovenia represented second and third cluster, respectively. The majority of variance was partitioned within colonies, and only a small but significant portion among clusters. Average relatedness within colony members was close to zero, did not differ significantly between the different colony types, and kinship is unlikely to be a major grouping mechanism in this species.",
journal = "PloS One",
title = "Population genetic structure of the Mediterranean horseshoe bat Rhinolophus euryale in the central Balkans.",
number = "1",
volume = "14",
doi = "10.1371/journal.pone.0210321",
pages = "e0210321"
}

Budinski, I., Blagojević, J., Jovanović, V., Bajić, B., Adnađević, T., Paunović, M.,& Vujošević, M.. (2019). Population genetic structure of the Mediterranean horseshoe bat Rhinolophus euryale in the central Balkans.. in PloS One, 14(1), e0210321.
https://doi.org/10.1371/journal.pone.0210321

Budinski I, Blagojević J, Jovanović V, Bajić B, Adnađević T, Paunović M, Vujošević M. Population genetic structure of the Mediterranean horseshoe bat Rhinolophus euryale in the central Balkans.. in PloS One. 2019;14(1):e0210321.
doi:10.1371/journal.pone.0210321 .

Budinski, Ivana, Blagojević, Jelena, Jovanović, Vladimir, Bajić, Branka, Adnađević, Tanja, Paunović, Milan, Vujošević, Mladen, "Population genetic structure of the Mediterranean horseshoe bat Rhinolophus euryale in the central Balkans." in PloS One, 14, no. 1 (2019):e0210321,
https://doi.org/10.1371/journal.pone.0210321 . .

TY  - CONF
AU  - Vidović, Biljana
AU  - Weyl, Philip
AU  - Marini, Francesca
AU  - Cristofaro, Massimo
AU  - Cvrković, Tatjana
AU  - Jojić, Vida
AU  - Petanović, Radmila
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5639
AB  - In the past few decades, increasing interest in the use of eriophyoid mites as biological control agents has been expressed, especially due to their high host specificity, high intrinsic rate of reproduction and damage frequently caused to reproductive plant parts. To date, three Metaculus species were registered as potential biological control agents and the necessity of more comprehensive understanding of taxonomic status of Metaculus rapistri has been emphasized (Vidović et al, 2015). Namely, M. rapistri was initially described by Carmona (1969) from samples of R. rugosum collected in Portugal. Thereafter, a supplementary morphological description of this species was provided by Monfreda and De Lillo (2012) from samples of Isatis tinctoria collected in Turkey. The goal of this study was to clarify the taxonomic status of mite species within the genus Metaculus that inhabit R. rugosum and I. tinctoria. 
	Plant samples of R. rugosum were from Portugal, while I. tinctoria were collected from Turkey and Germany. For the morphometric study, 28–30 females from each sample were examined in the dorso-ventral position. Twenty-two morphological traits of each individual were measured. The multivariate analysis of variance (MANOVA) and Canonical variate analysis (CVA) were used to examine morphological differences among groups. 
	DNA was extracted from 30 to 40 whole specimens using QIAGEN DNeasy Blood & Tissue Kit. Mitochondrial COI sequences were amplified using primers LCO1490/HCO2198. Uncorrected pairwise genetic distances were used to calculate the average genetic divergence between species with different host affiliations. 
	As evident from the CVA plot, the CV1 axis (accounting for 79.2% of variance) separated M. rapistri hosted by R. rugosum from Metaculus spp. hosted by I. tinctoria, while CV2 axis (accounting for 20.8% of variance) discriminated Metaculus sp. from I. tinctoria collected in Germany from Metaculus sp. from I. tinctoria collected in Turkey. The barcoding region of mtCOI gene indicated a genetic divergence between the analyzed populations of M. rapistri from R. rugosum and M. rapistri from I. tinctoria (7.7–11%). The genetic divergence was registered also between populations of mites from I. tinctoria from Turkey and the same plant species from Germany (12.3%). The results of our study showed that M. rapistri from R. rugosum is a distinct species and that could be two different taxa hosted by I. tinctoria.
PB  - Belgrade: Plant Protection Society of Serbia
C3  - Book of abstracts: VIII Congress on Plant Protection: Integrated Plant Protection for Sustainable Crop Production and Forestry; 2019 Nov 25-29; Zlatibor, Serbia
T1  - Morphological and molecular characterization of Metaculus eriophyid mites from Rapistrum rugosum and Isatis tinctoria (Brassicaceae)
SP  - 165
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5639
ER  - 

@conference{
author = "Vidović, Biljana and Weyl, Philip and Marini, Francesca and Cristofaro, Massimo and Cvrković, Tatjana and Jojić, Vida and Petanović, Radmila",
year = "2019",
abstract = "In the past few decades, increasing interest in the use of eriophyoid mites as biological control agents has been expressed, especially due to their high host specificity, high intrinsic rate of reproduction and damage frequently caused to reproductive plant parts. To date, three Metaculus species were registered as potential biological control agents and the necessity of more comprehensive understanding of taxonomic status of Metaculus rapistri has been emphasized (Vidović et al, 2015). Namely, M. rapistri was initially described by Carmona (1969) from samples of R. rugosum collected in Portugal. Thereafter, a supplementary morphological description of this species was provided by Monfreda and De Lillo (2012) from samples of Isatis tinctoria collected in Turkey. The goal of this study was to clarify the taxonomic status of mite species within the genus Metaculus that inhabit R. rugosum and I. tinctoria. 
	Plant samples of R. rugosum were from Portugal, while I. tinctoria were collected from Turkey and Germany. For the morphometric study, 28–30 females from each sample were examined in the dorso-ventral position. Twenty-two morphological traits of each individual were measured. The multivariate analysis of variance (MANOVA) and Canonical variate analysis (CVA) were used to examine morphological differences among groups. 
	DNA was extracted from 30 to 40 whole specimens using QIAGEN DNeasy Blood & Tissue Kit. Mitochondrial COI sequences were amplified using primers LCO1490/HCO2198. Uncorrected pairwise genetic distances were used to calculate the average genetic divergence between species with different host affiliations. 
	As evident from the CVA plot, the CV1 axis (accounting for 79.2% of variance) separated M. rapistri hosted by R. rugosum from Metaculus spp. hosted by I. tinctoria, while CV2 axis (accounting for 20.8% of variance) discriminated Metaculus sp. from I. tinctoria collected in Germany from Metaculus sp. from I. tinctoria collected in Turkey. The barcoding region of mtCOI gene indicated a genetic divergence between the analyzed populations of M. rapistri from R. rugosum and M. rapistri from I. tinctoria (7.7–11%). The genetic divergence was registered also between populations of mites from I. tinctoria from Turkey and the same plant species from Germany (12.3%). The results of our study showed that M. rapistri from R. rugosum is a distinct species and that could be two different taxa hosted by I. tinctoria.",
publisher = "Belgrade: Plant Protection Society of Serbia",
journal = "Book of abstracts: VIII Congress on Plant Protection: Integrated Plant Protection for Sustainable Crop Production and Forestry; 2019 Nov 25-29; Zlatibor, Serbia",
title = "Morphological and molecular characterization of Metaculus eriophyid mites from Rapistrum rugosum and Isatis tinctoria (Brassicaceae)",
pages = "165",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5639"
}

Vidović, B., Weyl, P., Marini, F., Cristofaro, M., Cvrković, T., Jojić, V.,& Petanović, R.. (2019). Morphological and molecular characterization of Metaculus eriophyid mites from Rapistrum rugosum and Isatis tinctoria (Brassicaceae). in Book of abstracts: VIII Congress on Plant Protection: Integrated Plant Protection for Sustainable Crop Production and Forestry; 2019 Nov 25-29; Zlatibor, Serbia
Belgrade: Plant Protection Society of Serbia., 165.
https://hdl.handle.net/21.15107/rcub_ibiss_5639

Vidović B, Weyl P, Marini F, Cristofaro M, Cvrković T, Jojić V, Petanović R. Morphological and molecular characterization of Metaculus eriophyid mites from Rapistrum rugosum and Isatis tinctoria (Brassicaceae). in Book of abstracts: VIII Congress on Plant Protection: Integrated Plant Protection for Sustainable Crop Production and Forestry; 2019 Nov 25-29; Zlatibor, Serbia. 2019;:165.
https://hdl.handle.net/21.15107/rcub_ibiss_5639 .

Vidović, Biljana, Weyl, Philip, Marini, Francesca, Cristofaro, Massimo, Cvrković, Tatjana, Jojić, Vida, Petanović, Radmila, "Morphological and molecular characterization of Metaculus eriophyid mites from Rapistrum rugosum and Isatis tinctoria (Brassicaceae)" in Book of abstracts: VIII Congress on Plant Protection: Integrated Plant Protection for Sustainable Crop Production and Forestry; 2019 Nov 25-29; Zlatibor, Serbia (2019):165,
https://hdl.handle.net/21.15107/rcub_ibiss_5639 .

TY  - CONF
AU  - Jojić, Vida
AU  - Čabrilo, Borislav
AU  - Vujošević, Mladen
AU  - Jovanović, Vladimir
AU  - Bjelić-Čabrilo, Olivera
AU  - Blagojević, Jelena
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5638
AB  - Developmental stability (DS), along with canalization, represents an element of developmental
homeostasis (DH). DH is defined as the mechanism responsible for ensuring phenotypic
constancy in organisms despite the great variability of genetic and environmental features.
DS is usually measured by fluctuating asymmetry (FA) which refers to the minor, random
differences between the two sides in bilaterally symmetric traits. The factors that cause
FA can be either genetic or environmental in origin. In this study we used 276 mandibles
and 323 crania of adult yellow-necked mice (Apodemus flavicollis) featured by the frequent
presence of supernumerary B chromosomes (Bs). We investigated the associations between
developmental stability and susceptibility to nematode parasitism in this species in the
context of Bs presence or absence. In agreement with prevailing view that Bs are genomic
parasites, B carriers would possess lower level of DS, i.e. higher level of FA, compared to noncarriers.
We hypothesized that parasitized individuals should be more asymmetric as well.
By applying landmark-based geometric morphometrics, we estimated the levels of FA (FA10a
indices) for mandibular size and shape and cranial shape in non-parasitized B non-carriers
(NPB0), parasitized B non-carriers (PB0), non-parasitized B carriers (NPB+) and parasitized B
carriers (PB+). According to the hypotheses mentioned above, NPB0 mice would possess the
lowest FA10a indices. Although our results revealed no significant differences in the levels
of FA, NPB0 individuals are characterized by the lowest FA10a index for mandibular size, but
the highest FA10a index for cranial shape. Additionally, the similar levels of FA estimated for
PB0 and PB+ mice indicated that B chromosomes are not linked to developmental stability
and susceptibility to nematode parasitism in this species.
PB  - Belgrade: Serbian Genetic Society
C3  - 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia
T1  - Developmental stability, B chromosomes and suspectibility to parasitism in the yellow-necked mouse Apodemus flavicollis
SP  - 139
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5638
ER  - 

@conference{
author = "Jojić, Vida and Čabrilo, Borislav and Vujošević, Mladen and Jovanović, Vladimir and Bjelić-Čabrilo, Olivera and Blagojević, Jelena",
year = "2019",
abstract = "Developmental stability (DS), along with canalization, represents an element of developmental
homeostasis (DH). DH is defined as the mechanism responsible for ensuring phenotypic
constancy in organisms despite the great variability of genetic and environmental features.
DS is usually measured by fluctuating asymmetry (FA) which refers to the minor, random
differences between the two sides in bilaterally symmetric traits. The factors that cause
FA can be either genetic or environmental in origin. In this study we used 276 mandibles
and 323 crania of adult yellow-necked mice (Apodemus flavicollis) featured by the frequent
presence of supernumerary B chromosomes (Bs). We investigated the associations between
developmental stability and susceptibility to nematode parasitism in this species in the
context of Bs presence or absence. In agreement with prevailing view that Bs are genomic
parasites, B carriers would possess lower level of DS, i.e. higher level of FA, compared to noncarriers.
We hypothesized that parasitized individuals should be more asymmetric as well.
By applying landmark-based geometric morphometrics, we estimated the levels of FA (FA10a
indices) for mandibular size and shape and cranial shape in non-parasitized B non-carriers
(NPB0), parasitized B non-carriers (PB0), non-parasitized B carriers (NPB+) and parasitized B
carriers (PB+). According to the hypotheses mentioned above, NPB0 mice would possess the
lowest FA10a indices. Although our results revealed no significant differences in the levels
of FA, NPB0 individuals are characterized by the lowest FA10a index for mandibular size, but
the highest FA10a index for cranial shape. Additionally, the similar levels of FA estimated for
PB0 and PB+ mice indicated that B chromosomes are not linked to developmental stability
and susceptibility to nematode parasitism in this species.",
publisher = "Belgrade: Serbian Genetic Society",
journal = "6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia",
title = "Developmental stability, B chromosomes and suspectibility to parasitism in the yellow-necked mouse Apodemus flavicollis",
pages = "139",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5638"
}

Jojić, V., Čabrilo, B., Vujošević, M., Jovanović, V., Bjelić-Čabrilo, O.,& Blagojević, J.. (2019). Developmental stability, B chromosomes and suspectibility to parasitism in the yellow-necked mouse Apodemus flavicollis. in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia
Belgrade: Serbian Genetic Society., 139.
https://hdl.handle.net/21.15107/rcub_ibiss_5638

Jojić V, Čabrilo B, Vujošević M, Jovanović V, Bjelić-Čabrilo O, Blagojević J. Developmental stability, B chromosomes and suspectibility to parasitism in the yellow-necked mouse Apodemus flavicollis. in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia. 2019;:139.
https://hdl.handle.net/21.15107/rcub_ibiss_5638 .

Jojić, Vida, Čabrilo, Borislav, Vujošević, Mladen, Jovanović, Vladimir, Bjelić-Čabrilo, Olivera, Blagojević, Jelena, "Developmental stability, B chromosomes and suspectibility to parasitism in the yellow-necked mouse Apodemus flavicollis" in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia (2019):139,
https://hdl.handle.net/21.15107/rcub_ibiss_5638 .

TY  - CONF
AU  - Blagojević, Jelena
AU  - Jovanović, Vladimir
AU  - Budinski, Ivana
AU  - Rajičić, Marija
AU  - Adnađević, Tanja
AU  - Vujošević, Mladen
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4850
AB  - Water, being of critical importance for the survival of life on the planet, requires our permanent care and attention. There is a growing concern about the genotoxicity of complex environmental mixtures present in surface waters, due to the risk of genetic damage and cancer, both in aquatic organisms and humans. The present study focuses on exploring the status of water pollution in areas with combined industrial and agricultural activities in order to estimate the magnitude of toxicity and genotoxicity using the Allium test. We collected samples from the Sava and Danube rivers, upstream and downstream of Šabac (Sava River) and Smederevo (Danube River). In both rivers sampling was done in periods of low and high water levels. Levels of toxicity were low in both rivers, although the presence of organic pollution was seen from higher root mean length in all sample groups than in negative controls. The highest values of genotoxicity at locations upstream of both Šabac and Smederevo were obtained in samples collected in a period of high water level, but only the upstream sample from the Sava River had reached a significant level of genotoxicity. This can be explained as a consequence of intensive agricultural activities. Our results indicate that communal town and industrial wastewaters influence river water quality more significantly during low level regimes, while high water levels increase the risk of exposure to chemicals used in agriculture.
PB  - Belgrade: Serbian Genetic Society
C3  - Book of Proceedings 6th Congress of Serbian Genetic Society
T1  - Influence of town wastewaters and agricultural activities on water genotoxicity during different river level regimes
SP  - 7
EP  - 14
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4850
ER  - 

@conference{
author = "Blagojević, Jelena and Jovanović, Vladimir and Budinski, Ivana and Rajičić, Marija and Adnađević, Tanja and Vujošević, Mladen",
year = "2019",
abstract = "Water, being of critical importance for the survival of life on the planet, requires our permanent care and attention. There is a growing concern about the genotoxicity of complex environmental mixtures present in surface waters, due to the risk of genetic damage and cancer, both in aquatic organisms and humans. The present study focuses on exploring the status of water pollution in areas with combined industrial and agricultural activities in order to estimate the magnitude of toxicity and genotoxicity using the Allium test. We collected samples from the Sava and Danube rivers, upstream and downstream of Šabac (Sava River) and Smederevo (Danube River). In both rivers sampling was done in periods of low and high water levels. Levels of toxicity were low in both rivers, although the presence of organic pollution was seen from higher root mean length in all sample groups than in negative controls. The highest values of genotoxicity at locations upstream of both Šabac and Smederevo were obtained in samples collected in a period of high water level, but only the upstream sample from the Sava River had reached a significant level of genotoxicity. This can be explained as a consequence of intensive agricultural activities. Our results indicate that communal town and industrial wastewaters influence river water quality more significantly during low level regimes, while high water levels increase the risk of exposure to chemicals used in agriculture.",
publisher = "Belgrade: Serbian Genetic Society",
journal = "Book of Proceedings 6th Congress of Serbian Genetic Society",
title = "Influence of town wastewaters and agricultural activities on water genotoxicity during different river level regimes",
pages = "7-14",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4850"
}

Blagojević, J., Jovanović, V., Budinski, I., Rajičić, M., Adnađević, T.,& Vujošević, M.. (2019). Influence of town wastewaters and agricultural activities on water genotoxicity during different river level regimes. in Book of Proceedings 6th Congress of Serbian Genetic Society
Belgrade: Serbian Genetic Society., 7-14.
https://hdl.handle.net/21.15107/rcub_ibiss_4850

Blagojević J, Jovanović V, Budinski I, Rajičić M, Adnađević T, Vujošević M. Influence of town wastewaters and agricultural activities on water genotoxicity during different river level regimes. in Book of Proceedings 6th Congress of Serbian Genetic Society. 2019;:7-14.
https://hdl.handle.net/21.15107/rcub_ibiss_4850 .

Blagojević, Jelena, Jovanović, Vladimir, Budinski, Ivana, Rajičić, Marija, Adnađević, Tanja, Vujošević, Mladen, "Influence of town wastewaters and agricultural activities on water genotoxicity during different river level regimes" in Book of Proceedings 6th Congress of Serbian Genetic Society (2019):7-14,
https://hdl.handle.net/21.15107/rcub_ibiss_4850 .

TY  - CONF
AU  - Blagojević, Jelena
AU  - Jovanović, Vladimir
AU  - Budinski, Ivana
AU  - Rajičić, Marija
AU  - Adnađević, Tanja
AU  - Vujošević, Mladen
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4846
AB  - Water, being of critical importance for the survival of life on the planet, requires our
permanent care and attention. There is a growing concern about the genotoxicity of complex
environmental mixtures present in surface waters, due to the risk of genetic damage and
cancer, both in aquatic organisms and humans. Allium anaphase-telophase test has been
accepted as a promising tool to detect, among other things, toxicity and genotoxicity of river
water. The present study has focused on exploring the status of water pollution in areas
with combined industrial and agricultural activities in order to estimate the magnitude
of toxicity and genotoxicity using the Allium test. We collected samples of surface water
from the Sava River, upstream and downstream from the town of Šabaca nd at the Danube
River upstream and downstream from the town Smederevo. We analyzed using the Allium
anaphase-telophase test. In both rivers sampling was done in periods of low and high water
level. While at low water level genotoxicity was higher at points downstream of Šabaca nd
Smederevo, samples collected in periods of highwater level were more genotoxic upstream
from the towns. This can be explained as a consequence of agricultural activities.
PB  - Belgrade: Serbian Genetic Society
C3  - 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia
T1  - Influence of town pollutions on levels of water genotoxicity during different water level regimes
SP  - 129
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4846
ER  - 

@conference{
author = "Blagojević, Jelena and Jovanović, Vladimir and Budinski, Ivana and Rajičić, Marija and Adnađević, Tanja and Vujošević, Mladen",
year = "2019",
abstract = "Water, being of critical importance for the survival of life on the planet, requires our
permanent care and attention. There is a growing concern about the genotoxicity of complex
environmental mixtures present in surface waters, due to the risk of genetic damage and
cancer, both in aquatic organisms and humans. Allium anaphase-telophase test has been
accepted as a promising tool to detect, among other things, toxicity and genotoxicity of river
water. The present study has focused on exploring the status of water pollution in areas
with combined industrial and agricultural activities in order to estimate the magnitude
of toxicity and genotoxicity using the Allium test. We collected samples of surface water
from the Sava River, upstream and downstream from the town of Šabaca nd at the Danube
River upstream and downstream from the town Smederevo. We analyzed using the Allium
anaphase-telophase test. In both rivers sampling was done in periods of low and high water
level. While at low water level genotoxicity was higher at points downstream of Šabaca nd
Smederevo, samples collected in periods of highwater level were more genotoxic upstream
from the towns. This can be explained as a consequence of agricultural activities.",
publisher = "Belgrade: Serbian Genetic Society",
journal = "6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia",
title = "Influence of town pollutions on levels of water genotoxicity during different water level regimes",
pages = "129",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4846"
}

Blagojević, J., Jovanović, V., Budinski, I., Rajičić, M., Adnađević, T.,& Vujošević, M.. (2019). Influence of town pollutions on levels of water genotoxicity during different water level regimes. in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia
Belgrade: Serbian Genetic Society., 129.
https://hdl.handle.net/21.15107/rcub_ibiss_4846

Blagojević J, Jovanović V, Budinski I, Rajičić M, Adnađević T, Vujošević M. Influence of town pollutions on levels of water genotoxicity during different water level regimes. in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia. 2019;:129.
https://hdl.handle.net/21.15107/rcub_ibiss_4846 .

Blagojević, Jelena, Jovanović, Vladimir, Budinski, Ivana, Rajičić, Marija, Adnađević, Tanja, Vujošević, Mladen, "Influence of town pollutions on levels of water genotoxicity during different water level regimes" in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia (2019):129,
https://hdl.handle.net/21.15107/rcub_ibiss_4846 .

TY  - THES
AU  - Rajičić, Marija
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3374
AB  - B hromozomi predstavljaju prekobrojne hromozome koji nisu neophodni za normalan rast i razviće. Ovi prekobrojni hromozomi se javljaju kod svih višećelijskih eukariotskih grupa, osim kod ptica, i različiti su po poreklu, morfologiji, veličini, broju, genetičkoj strukturi i efektima na fenotip. Njihova prisutnost u živom svetu procenjuje se na 3% kariotipski analiziranih vrsta. Karakteriše ih nasleđivanje koje ne prati Mendelova pravila, kao i izostanak rekombinacije sa hromozomima osnovnog A seta. Glavna pitanja koja se postavljaju u savremenim istraživanjima B hromozoma se odnose na njihovo poreklo, mehanizme nastanka i održavanja. Poznavanje genetičkog sadržaja, transkripcionog statusa i efekata prekobrojnih hromozoma, omogućuje rasvetljavanje njihovog biološkog značaja. Glodari su najbrojniji nosioci B hromozoma među sisarima. Rod Apodemus broji ukupno 20 vrsta, od kojih je kod šest zabeleženo prisustvo B hromozoma. U ovoj studiji izučavani su B hromozomi na jedinkama vrste žutogrli miš, Apodemus flavicollis. Nosioci imaju najčešće jedan ili dva B, dok je maksimalan zabeležen broj osam B hromozoma. Prekobrojni hromozomi se kod ove vrste javljaju u svim do sada proučavanim populacijama na teritoriji Republike Srbije, sa različitom učestalošću. Brojne populacione studije na ovoj vrsti pokazuju da jedinke sa B hromozomima imaju višu adaptivnu vrednost u poređenju sa jedinkama bez prekobrojnih hromozoma. Cilj ove studije bio je utvrđivanje sekvence, proučavanje porekla, kao i analiza transkripcione aktivnosti pojedinih sekvenci B hromozoma vrste A. flavicollis. U istraživanje su uključene životinje sa lokaliteta u Republici Srbiji, Ruskoj federaciji i Republici Belorusiji. Metodom mikrodisekcije iz kariotipa tri jedinke su izolovani B hromozomi koji su potom umnoženi u lančanoj reakciji polimeraze (PCR). Proizvedene su fluorescentne B-specifične probe. Procena homologije između B-specifičnih proba i hromozoma osnovnog kariotipa iste vrste izvršena je putem fluorescentne in situ hibridizacije (FISH). Utvrđena je homologija između sekvence B hromozoma i: a) pericentromernog regiona (PR) polnih hromozoma, b) subtelomernih regiona dva para malih autozoma, kao i c) slabija homologija sa distalnim regionom Y hromozoma. Homologija između sekvenci različitih B hromozoma je potvrđena bez obzira na geografsku udaljenost populacija iz kojih uzorci vode poreklo i nezavisno od broja B hromozoma u kariotipu. Ovo ukazuje na isto poreklo i/ili strukturu svih B hromozoma kod vrste A. flavicollis. U ovoj studiji je takođe pokazano da i pored česte zastupljenosti C-kit gena na B hromozomima različitih vrsta sisara, ovaj gen nije pronađen na B hromozomima vrste A. flavicollis. Poređenjem sekvenci B hromozoma sa referentnim genomom vrste Mus musculus, identifikovano je 39 gena sa 22 hromozomska regiona koja vode poreklo sa 16 hromozoma referentnog genoma. Sa izuzetkom B hromozoma poreklom iz Ruske federacije, koji u sekvenci sadrži jedan dodatni hromozomski region koji nije nađen kod populacija iz Republike Srbije, rezultati pokazuju da su svi analizirani B hromozomi izgrađeni od istih regiona hromozoma A seta. Sekvenca B hromozoma je u najvećoj meri sadrži gene koji kodiraju proteine mikrotubula i ćelijskog ciklusa. Takođe su prisutni i geni koji kodiraju nukleotid-vezujuće, metal-vezujuće proteine kao i proteine membrane, identifikovan je i veliki broj repetitivnih sekvenci, među kojima su transpozoni u nekim slučajevima bili tandemski organizovani. Utvrđeno je i da se geni smešteni na B hromozomima nalaze u različitim stadijumima degradacije. Nasuprot višedecenijskom uverenju da su B hromozomi genetički inertni, u ovom istraživanju je pokazano da se pseudogeni poreklom sa B hromozoma transkribuju. Pored toga, neizmenjeni geni sa B hromozoma, ili sa osnovnog genoma, u prisustvu B hromozoma, pokazuju viši nivo transkripcije kod ženki u mlađem životnom dobu. Transkripcija gena Cenpe je povezana i sa brojem B hromozoma, tako da je nivo transkripcije najviši kod jedinki koje imaju jedan B hromozom. B hromozomi vrste A. flavicollis, najverovatnije vode poreklo od pericentromernog regiona X hromozoma koji se nalazi neposredno uz pseudoautozomalni region, odlikuju se stabilnom genetičkom strukturom na širokom arealu vrste, transkripciono su aktivni na način koji je povezan sa polom, starošću i brojem B hromozoma u kariotipu.
AB  - B chromosomes (Bs) are supernumerary chromosomes in a standard karyotype, unnecessary for normal growth and development. Bs are present in all main groups of multicellular eukaryotes, except birds, and extremely variable considering origin, morphology, size, number, molecular structure, and phenotypic effects. 3% of all analyzed species have B chromosomes. The Bs do not follow rules of Mendelian segregation law and do not recombine with members of the basic A chromosome complement. The main questions considered in contemporary studies of Bs are surrounding their origin, mechanisms of formation and maintenance. Knowledge of their molecular structure, transcriptional activity, and phenotypic effects, will allow a deeper understanding of their biological importance. Within mammals, the most frequent carriers of Bs are rodents. The genus Apodemus includes 20 species, six of which carry Bs, in addition to A chromosome set. In this study, B chromosomes were analyzed in specimens of the yellow-necked mouse, Apodemus flavicollis. Carriers usually have one or two Bs, and the highest recorded number in this species is eight B chromosomes. In different frequencies, supernumeraries are present in all studied populations of A. flavicollis in the territory of the Republic of Serbia. Numerous population studies of this species are indicating that B carriers have a higher fitness compared to individuals without B chromosomes. The aim of this study was to define sequence, origin and transcriptional activity of selected B regions in species A. flavicollis. Specimens were collected from different localities in the Republic of Serbia, Russian Federation and the Republic of Belarus. B chromosomes have been isolated by microdissection from the karyotype of three individuals and amplified in the polymerase chain reaction (PCR). B-specific fluorescent probes were synthesized. Estimation of homology between B-specific probes and chromosomes of A set had been obtained by fluorescent in situ hybridization (FISH). Homology was shown between the B chromosome and: a) pericentromeric region (PR) of sex chromosomes b) subtelomeric region of the two pairs of small chromosomes and c) lower homology with the distal region of the Y chromosome. Despite the distinct geographical origin of samples and different number of Bs in karyotypes, hybridized probes showed the same affinity to Bs. This indicates the same origin and/or structure of all Bs in A. flavicollis. This study has demonstrated that C-kit gene does not contribute to the composition of supernumeraries in A. flavicollis, contrary to the studies of Bs in numerous mammalian species. Comparing sequences of Bs and referent genome of the species Mus musculus, 39 genes in 22 chromosomal regions that originate from 16 chromosomes of the referent genome have been identified. With the exception of one region present on B chromosome from Russian Federation, that contained one additional chromosomal region not present on the samples from Serbian populations, results demonstrate that all analyzed Bs are composed of the same A-chromosomes’ regions. B chromosomes are rich in microtubule and cell cycle protein-coding genes. Other present categories include genes that are coding nucleotide-binding, membrane, and metal-binding proteins, as well as a high amount of repetitive elements. Transposable elements were tandemly arranged in some cases. Genes present on B chromosomes are at different stages of degradation. In contrast to the view accepted for many decades that Bs are genetically inert elements, this study demonstrates that pseudogenes on Bs are transcribed. The study also demonstrates that Bs-genes, or standard-genome genes in the presence of Bs, show higher transcriptional levels in young females. Furthermore, transcription of Cenpe gene is linked with the number of Bs, in the manner that samples with one B show the highest transcription level. B chromosomes in A. flavicollis probably originate from the pericentromeric region of the X chromosome which is positioned near pseudoautosomal region, they are featured with stable genetic structure throughout the whole areal of the species and are transcriptionally active depending on the sex, age and number of Bs.
PB  - Belgrade: University of Belgrade, Faculty of Biology
T2  - University of Belgrade, Faculty of Biology
T1  - Molecular structure, origin and function of B chromosomes in yellow-necked wood mouse Apodemus flavicollis(Mammalia, Rodentia)
T1  - Molekularna struktura, poreklo i funkcija B hromozoma kod žutogrlog šumskog miša Apodemus flavicollis (Mammalia, Rodentia)
SP  - 1
EP  - 90
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3374
ER  - 

@phdthesis{
author = "Rajičić, Marija",
year = "2019",
abstract = "B hromozomi predstavljaju prekobrojne hromozome koji nisu neophodni za normalan rast i razviće. Ovi prekobrojni hromozomi se javljaju kod svih višećelijskih eukariotskih grupa, osim kod ptica, i različiti su po poreklu, morfologiji, veličini, broju, genetičkoj strukturi i efektima na fenotip. Njihova prisutnost u živom svetu procenjuje se na 3% kariotipski analiziranih vrsta. Karakteriše ih nasleđivanje koje ne prati Mendelova pravila, kao i izostanak rekombinacije sa hromozomima osnovnog A seta. Glavna pitanja koja se postavljaju u savremenim istraživanjima B hromozoma se odnose na njihovo poreklo, mehanizme nastanka i održavanja. Poznavanje genetičkog sadržaja, transkripcionog statusa i efekata prekobrojnih hromozoma, omogućuje rasvetljavanje njihovog biološkog značaja. Glodari su najbrojniji nosioci B hromozoma među sisarima. Rod Apodemus broji ukupno 20 vrsta, od kojih je kod šest zabeleženo prisustvo B hromozoma. U ovoj studiji izučavani su B hromozomi na jedinkama vrste žutogrli miš, Apodemus flavicollis. Nosioci imaju najčešće jedan ili dva B, dok je maksimalan zabeležen broj osam B hromozoma. Prekobrojni hromozomi se kod ove vrste javljaju u svim do sada proučavanim populacijama na teritoriji Republike Srbije, sa različitom učestalošću. Brojne populacione studije na ovoj vrsti pokazuju da jedinke sa B hromozomima imaju višu adaptivnu vrednost u poređenju sa jedinkama bez prekobrojnih hromozoma. Cilj ove studije bio je utvrđivanje sekvence, proučavanje porekla, kao i analiza transkripcione aktivnosti pojedinih sekvenci B hromozoma vrste A. flavicollis. U istraživanje su uključene životinje sa lokaliteta u Republici Srbiji, Ruskoj federaciji i Republici Belorusiji. Metodom mikrodisekcije iz kariotipa tri jedinke su izolovani B hromozomi koji su potom umnoženi u lančanoj reakciji polimeraze (PCR). Proizvedene su fluorescentne B-specifične probe. Procena homologije između B-specifičnih proba i hromozoma osnovnog kariotipa iste vrste izvršena je putem fluorescentne in situ hibridizacije (FISH). Utvrđena je homologija između sekvence B hromozoma i: a) pericentromernog regiona (PR) polnih hromozoma, b) subtelomernih regiona dva para malih autozoma, kao i c) slabija homologija sa distalnim regionom Y hromozoma. Homologija između sekvenci različitih B hromozoma je potvrđena bez obzira na geografsku udaljenost populacija iz kojih uzorci vode poreklo i nezavisno od broja B hromozoma u kariotipu. Ovo ukazuje na isto poreklo i/ili strukturu svih B hromozoma kod vrste A. flavicollis. U ovoj studiji je takođe pokazano da i pored česte zastupljenosti C-kit gena na B hromozomima različitih vrsta sisara, ovaj gen nije pronađen na B hromozomima vrste A. flavicollis. Poređenjem sekvenci B hromozoma sa referentnim genomom vrste Mus musculus, identifikovano je 39 gena sa 22 hromozomska regiona koja vode poreklo sa 16 hromozoma referentnog genoma. Sa izuzetkom B hromozoma poreklom iz Ruske federacije, koji u sekvenci sadrži jedan dodatni hromozomski region koji nije nađen kod populacija iz Republike Srbije, rezultati pokazuju da su svi analizirani B hromozomi izgrađeni od istih regiona hromozoma A seta. Sekvenca B hromozoma je u najvećoj meri sadrži gene koji kodiraju proteine mikrotubula i ćelijskog ciklusa. Takođe su prisutni i geni koji kodiraju nukleotid-vezujuće, metal-vezujuće proteine kao i proteine membrane, identifikovan je i veliki broj repetitivnih sekvenci, među kojima su transpozoni u nekim slučajevima bili tandemski organizovani. Utvrđeno je i da se geni smešteni na B hromozomima nalaze u različitim stadijumima degradacije. Nasuprot višedecenijskom uverenju da su B hromozomi genetički inertni, u ovom istraživanju je pokazano da se pseudogeni poreklom sa B hromozoma transkribuju. Pored toga, neizmenjeni geni sa B hromozoma, ili sa osnovnog genoma, u prisustvu B hromozoma, pokazuju viši nivo transkripcije kod ženki u mlađem životnom dobu. Transkripcija gena Cenpe je povezana i sa brojem B hromozoma, tako da je nivo transkripcije najviši kod jedinki koje imaju jedan B hromozom. B hromozomi vrste A. flavicollis, najverovatnije vode poreklo od pericentromernog regiona X hromozoma koji se nalazi neposredno uz pseudoautozomalni region, odlikuju se stabilnom genetičkom strukturom na širokom arealu vrste, transkripciono su aktivni na način koji je povezan sa polom, starošću i brojem B hromozoma u kariotipu., B chromosomes (Bs) are supernumerary chromosomes in a standard karyotype, unnecessary for normal growth and development. Bs are present in all main groups of multicellular eukaryotes, except birds, and extremely variable considering origin, morphology, size, number, molecular structure, and phenotypic effects. 3% of all analyzed species have B chromosomes. The Bs do not follow rules of Mendelian segregation law and do not recombine with members of the basic A chromosome complement. The main questions considered in contemporary studies of Bs are surrounding their origin, mechanisms of formation and maintenance. Knowledge of their molecular structure, transcriptional activity, and phenotypic effects, will allow a deeper understanding of their biological importance. Within mammals, the most frequent carriers of Bs are rodents. The genus Apodemus includes 20 species, six of which carry Bs, in addition to A chromosome set. In this study, B chromosomes were analyzed in specimens of the yellow-necked mouse, Apodemus flavicollis. Carriers usually have one or two Bs, and the highest recorded number in this species is eight B chromosomes. In different frequencies, supernumeraries are present in all studied populations of A. flavicollis in the territory of the Republic of Serbia. Numerous population studies of this species are indicating that B carriers have a higher fitness compared to individuals without B chromosomes. The aim of this study was to define sequence, origin and transcriptional activity of selected B regions in species A. flavicollis. Specimens were collected from different localities in the Republic of Serbia, Russian Federation and the Republic of Belarus. B chromosomes have been isolated by microdissection from the karyotype of three individuals and amplified in the polymerase chain reaction (PCR). B-specific fluorescent probes were synthesized. Estimation of homology between B-specific probes and chromosomes of A set had been obtained by fluorescent in situ hybridization (FISH). Homology was shown between the B chromosome and: a) pericentromeric region (PR) of sex chromosomes b) subtelomeric region of the two pairs of small chromosomes and c) lower homology with the distal region of the Y chromosome. Despite the distinct geographical origin of samples and different number of Bs in karyotypes, hybridized probes showed the same affinity to Bs. This indicates the same origin and/or structure of all Bs in A. flavicollis. This study has demonstrated that C-kit gene does not contribute to the composition of supernumeraries in A. flavicollis, contrary to the studies of Bs in numerous mammalian species. Comparing sequences of Bs and referent genome of the species Mus musculus, 39 genes in 22 chromosomal regions that originate from 16 chromosomes of the referent genome have been identified. With the exception of one region present on B chromosome from Russian Federation, that contained one additional chromosomal region not present on the samples from Serbian populations, results demonstrate that all analyzed Bs are composed of the same A-chromosomes’ regions. B chromosomes are rich in microtubule and cell cycle protein-coding genes. Other present categories include genes that are coding nucleotide-binding, membrane, and metal-binding proteins, as well as a high amount of repetitive elements. Transposable elements were tandemly arranged in some cases. Genes present on B chromosomes are at different stages of degradation. In contrast to the view accepted for many decades that Bs are genetically inert elements, this study demonstrates that pseudogenes on Bs are transcribed. The study also demonstrates that Bs-genes, or standard-genome genes in the presence of Bs, show higher transcriptional levels in young females. Furthermore, transcription of Cenpe gene is linked with the number of Bs, in the manner that samples with one B show the highest transcription level. B chromosomes in A. flavicollis probably originate from the pericentromeric region of the X chromosome which is positioned near pseudoautosomal region, they are featured with stable genetic structure throughout the whole areal of the species and are transcriptionally active depending on the sex, age and number of Bs.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "University of Belgrade, Faculty of Biology",
title = "Molecular structure, origin and function of B chromosomes in yellow-necked wood mouse Apodemus flavicollis(Mammalia, Rodentia), Molekularna struktura, poreklo i funkcija B hromozoma kod žutogrlog šumskog miša Apodemus flavicollis (Mammalia, Rodentia)",
pages = "1-90",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3374"
}

Rajičić, M.. (2019). Molecular structure, origin and function of B chromosomes in yellow-necked wood mouse Apodemus flavicollis(Mammalia, Rodentia). in University of Belgrade, Faculty of Biology
Belgrade: University of Belgrade, Faculty of Biology., 1-90.
https://hdl.handle.net/21.15107/rcub_ibiss_3374

Rajičić M. Molecular structure, origin and function of B chromosomes in yellow-necked wood mouse Apodemus flavicollis(Mammalia, Rodentia). in University of Belgrade, Faculty of Biology. 2019;:1-90.
https://hdl.handle.net/21.15107/rcub_ibiss_3374 .

Rajičić, Marija, "Molecular structure, origin and function of B chromosomes in yellow-necked wood mouse Apodemus flavicollis(Mammalia, Rodentia)" in University of Belgrade, Faculty of Biology (2019):1-90,
https://hdl.handle.net/21.15107/rcub_ibiss_3374 .

TY  - JOUR
AU  - Kačar, Tamara
AU  - Stamenković, Gorana
AU  - Blagojević, Jelena
AU  - Krtinić, Jovica
AU  - Mijović, Dragan
AU  - Marjanović, Damir
PY  - 2019
UR  - https://www.tandfonline.com/doi/full/10.1080/03014460.2019.1584242
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3344
AB  - BACKGROUND Serbs mainly live in the territory of the recently re-established state of Serbia. However, the turbulent history in the Balkan Peninsula has led to settlement of Serbs not only within present day Serbia, but also in different parts of neighbouring countries. AIM To define polymorphisms of 23 Y-chromosomal short tandem repeat (STR) loci in a modern Serbian population from the central part of the Balkan Peninsula. SUBJECTS AND METHODS The reference sample consisted of 303 men declared as Serbs over three generations. Localities of the collected materials include the territories of Serbia, Bosnia and Herzegovina, Croatia and Montenegro. DNA samples were typed using the PowerPlex®Y23 amplification kit. RESULTS The highest locus diversity was observed for DYS385 and DYS481. In this study the most abundant haplogroups were I2a, E1b1b, R1a and I1. The largest genetic distances between the Serbs and other close Southern Slavs were for the Macedonians and Slovenians. CONCLUSION This study is the first one to define STR polymorphism of Serbian people not only from Serbia but also from other parts of the Balkan Peninsula. The presented genetic data may be useful in further examinations of the genesis and genetic structuring of the present-day Serbian gene pool.
T2  - Annals of Human Biology
T1  - Y chromosome genetic data defined by 23 short tandem repeats in a Serbian population on the Balkan Peninsula.
IS  - 1
VL  - 46
DO  - 10.1080/03014460.2019.1584242
SP  - 77
EP  - 83
ER  - 

@article{
author = "Kačar, Tamara and Stamenković, Gorana and Blagojević, Jelena and Krtinić, Jovica and Mijović, Dragan and Marjanović, Damir",
year = "2019",
abstract = "BACKGROUND Serbs mainly live in the territory of the recently re-established state of Serbia. However, the turbulent history in the Balkan Peninsula has led to settlement of Serbs not only within present day Serbia, but also in different parts of neighbouring countries. AIM To define polymorphisms of 23 Y-chromosomal short tandem repeat (STR) loci in a modern Serbian population from the central part of the Balkan Peninsula. SUBJECTS AND METHODS The reference sample consisted of 303 men declared as Serbs over three generations. Localities of the collected materials include the territories of Serbia, Bosnia and Herzegovina, Croatia and Montenegro. DNA samples were typed using the PowerPlex®Y23 amplification kit. RESULTS The highest locus diversity was observed for DYS385 and DYS481. In this study the most abundant haplogroups were I2a, E1b1b, R1a and I1. The largest genetic distances between the Serbs and other close Southern Slavs were for the Macedonians and Slovenians. CONCLUSION This study is the first one to define STR polymorphism of Serbian people not only from Serbia but also from other parts of the Balkan Peninsula. The presented genetic data may be useful in further examinations of the genesis and genetic structuring of the present-day Serbian gene pool.",
journal = "Annals of Human Biology",
title = "Y chromosome genetic data defined by 23 short tandem repeats in a Serbian population on the Balkan Peninsula.",
number = "1",
volume = "46",
doi = "10.1080/03014460.2019.1584242",
pages = "77-83"
}

Kačar, T., Stamenković, G., Blagojević, J., Krtinić, J., Mijović, D.,& Marjanović, D.. (2019). Y chromosome genetic data defined by 23 short tandem repeats in a Serbian population on the Balkan Peninsula.. in Annals of Human Biology, 46(1), 77-83.
https://doi.org/10.1080/03014460.2019.1584242

Kačar T, Stamenković G, Blagojević J, Krtinić J, Mijović D, Marjanović D. Y chromosome genetic data defined by 23 short tandem repeats in a Serbian population on the Balkan Peninsula.. in Annals of Human Biology. 2019;46(1):77-83.
doi:10.1080/03014460.2019.1584242 .

Kačar, Tamara, Stamenković, Gorana, Blagojević, Jelena, Krtinić, Jovica, Mijović, Dragan, Marjanović, Damir, "Y chromosome genetic data defined by 23 short tandem repeats in a Serbian population on the Balkan Peninsula." in Annals of Human Biology, 46, no. 1 (2019):77-83,
https://doi.org/10.1080/03014460.2019.1584242 . .

TY  - THES
AU  - Budinski, Ivana
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3557
AB  - The population genetic structure of a species is a result of past events such as bottlenecks, population expansion and secondary contact, as well as recent events and phenomena like migrations, philopatry, sociality etc. Climatic oscillations during the Pleistocene shaped the present distribution of genetic diversity of temperate biota in Europe. The Balkan peninsula has been recognized as a glacial refugium for many temperate species, including many bat species. The Mediterranean horseshoe bat Rhinolophus euryale is distributed in the Mediterranean area. It is predominantly cave-dwelling species and its distribution greatly overlaps with karstic regions. During summer females form nursery colonies where they give birth and rear pups, while in autumn both sexes gather together into hibernation roosts. Roost fidelity has been found in both sexes. R. euryale is considered to be a sedentary species, since the distance between summer and winter roosts is rarely greater than 50 km. Distribution of this species in Serbia overlaps with karstic areas in western and eastern Serbia. The aims of this thesis were to gather all available data about species’ distribution and to evaluate population trends in Serbia; testing ISSR-PCR method for reliable distinguishing this species from morphologically similar R. blasii. D-loop mitochondrial sequences were used for analyses of genetic diversity, demographic history and phylogeography of the Mediterranean horseshoe bat in the Balkan peninsula. Genetic variability of 12 populations in the central Balkans was analysed using nuclear microsatellites. Genetic differentiation and gene flow among populations from different geographic regions were estimated, and relatedness within different colony types was assessed. In the period from 2012 to 2017, R. euryale was found at 26 localities in Serbia (11 localities for the first time), and now the number of localities counts 73. Several new nursery and hibernation colonies of this species were found, as well as one male summer colony. Only one roost was found in a building, while the rest were in underground sites. No significant threats were identified, and the population trend of this species in Serbia is estimated to be stable. ISSR-PCR represents fast and reliable method for unambiguously distinguishing morphologically similar medium-sized horseshoe species R. euryale and R. blasii. This study represents first population genetic analysis of the Mediterranean horseshoe bat in the central Balkans. High haplotype diversity of mitochondrial D-loop sequences was found, and all samples from Europe, Anatolia and Northern Africa clustered within one phylogenetic clade. Obtained results support the scenario of a sudden demographic expansion, and it is estimated that this started in the late Pleistocene. The Balkan peninsula was probably one of the glacial refugia of this species in Europe. High genetic diversity was also found using nuclear microsatellites. Moderate genetic substructuring among geographic regions and significant isolation by distance were observed. Genetic differentiation among populations from western and eastern Serbia was small but significant, which can be explained by roost fidelity and non-migratory behaviour of the species. The population from Montenegro was genetically similar to ones from eastern Serbia, which implicates the potential existence of colonies between the analysed regions. Kinship is probably not a major factor driving grouping of females into nursery colonies, but, more likely, benefits from group thermoregulation and information transfer about suitable roosts and foraging areas. Information transfer is likely underlying the formation of male summer colonies. High genetic diversity was found among Mediterranean horseshoe bat populations in the central parts of the Balkan peninsula, both for nuclear and mitochondrial markers. These populations harbour significant portion of species’ genetic diversity, and they have been recognized as the most numerous and the most stable across the distribution of R. euryale. Therefore, protecting Balkan populations might play a vital role in conservation of Mediterranean horseshoe bat.
AB  - Genetička struktura populacija predstavlja rezultat efekta prošlih događaja kao što su prolazak populacija kroz usko grlo, postglacijalna ekspanzija, sekundarni kontakt, ali i recentnih događaja i fenomena koji obuhvataju migracije, filopatriju, socijalnost itd. Pleistocenske klimatske oscilacije uticale su na današnju distribuciju genetičkog diverziteta mnogih vrsta koje naseljavaju Evropu. Balkansko poluostrvo je prepoznato kao glacijalni refugijum za veliki broj vrsta umerenih područja, uključujući i određene vrste slepih miševa. Sredozemni potkovičar Rhinolophus euryale je rasprostranjen na širem području oko Sredozemnog mora. Najčešće koristi podzemna skloništa kao što su pećine, i zbog toga je njegova distribucija vezana za krečnjačke predele. Tokom leta se ženke grupišu u porodiljske kolonije gde se odvija koćenje i podizanje mladih, a jedinke oba pola se u jesen okupljaju u hibernacijske kolonije. Filopatrija je zabeležena kod jedinki oba pola. R. euryale se smatra sedentarnom vrstom jer između letnjih i zimskih skloništa retko prelazi udaljenosti veće od 50 km. U Srbiji se distribucija ove vrste poklapa sa krečnjačkim područjima u zapadnoj i istočnoj Srbiji. Ciljevi ove doktorske disertacije bili su sistematizacija svih podataka o rasprostranjenosti ove vrste i evaluacija populacionih trendova u Srbiji; testiranje ISSR-PCR metode za brzo i pouzdano razlikovanje ove vrste i morfološki slične vrste R. blasii. Sekvence mitohondrijske D-petlje su korišćene za analizu genetičke varijabilnosti, demografske istorije i filogeografije ove vrste na Balkanskom poluostrvu. Pomoću mikrosatelitskih markera analizirana je genetička varijabilnost ove vrste u 12 populacija na području centralnog Balkana, testirana je genetička diferenciranost i procenjen protok gena između geografskih regiona. Pored toga, analiziran je stepen srodstva između jedinki unutar različitih tipova kolonija. U periodu od 2012 do 2017. godine, sredozemni potkovičar je zabeležen na ukupno 26 lokaliteta u Srbiji (na 11 je zabeležen prvi put). Ovime je broj lokaliteta u Srbiji sa dokazanim prisustvom istraživane vrste porastao na 73. Pronađeno je nekoliko novih porodiljskih i hibernacijskih kolonija, kao i jedna letnja kolonija mužjaka. Zabeleženo je samo jedno sklonište u građevini, dok su sva ostala bila u podzemnim objektima. Nisu identifikovani značajni faktori ugrožavanja, i procenjeno je da ova vrsta u Srbiji ima stabilan populacioni trend. Upotrebom ISSR-PCR markera je moguće brzo i sa velikom pouzdanošću razlikovati morfološki slične vrste srednjih potkovičara R. euryale i R. blasii. Ova studija predstavlja prvu populaciono-genetičku analizu sredozemnog potkovičara na području centralnog Balkana. Zabeležen je veliki diverzitet haplotipova D-petlje mitohondrijske DNK, i svi uzorci sa područja Evrope, Anadolije i Severne Afrike su se grupisali u jednu filogenetsku kladu. Dobijeni rezultati podržavaju scenario brze demografske ekspanzije, a procenjeno je da je ekspanzija počela krajem Pleistocena. Balkansko poluostrvo je najverovatnije predstavljalo jedan od refugijuma ove vrste u Evropi. Upotrebom mikrosatelitskih markera je takođe zabeležen veliki genetički diverzitet. Na analiziranom području postoji umereni nivo diferencijacije između geografskih regiona, i značajna izolacija putem distance. Genetička diferenciranost između populacija u zapadnoj i istočnoj Srbiji je bila mala ali značajna, što se može objasniti sedentarnošću i filopatrijom vrste. Populacija iz Crne Gore je genetički slična populacijama iz istočne Srbije, što ukazuje na potencijalno postojanje kolonija između tih regiona. Pokazano je da je stepen srodstva između jedinki unutar različitih tipova kolonija sličan. Mehanizmi koji leže u osnovi formiranja porodiljskih kolonija su najverovatnije korist od grupne termoregulacije i prenos informacija o adekvatnim skloništima i lovnim teritorijama. Razmena informacija je takođe verovatno jedan od razloga grupisanja mužjaka u letnje kolonije. U Balkanskim populacijama sredozemnog potkovičara zabeležen je veliki genetički diverzitet, kako na nuklearnom, tako i na mitohondrijskom nivou. Ove populacije sadrže značajni deo genetičkog diverziteta vrste, i prepoznate su kao najbrojnije i najstabilnije na celom arealu R. euryale. Očuvanje i zaštita balkanskih populacija sredozemnog potkovičara mogu imati krucijalni značaj u opstanku ove vrste.
PB  - Belgrade: University of Belgrade, Faculty of Biology
T2  - University of Belgrade, Faculty of Biology
T1  - Genetička struktura populacija sredozemnog potkovičara Rhinolophus euryale Blasius, 1853 u Srbiji i filogeografija ove vrste na Balkanskom poluostrvu
T1  - Population genetic structure of the Mediterranean horseshoe bat Rhinolophus euryale Blasius, 1853 in Serbia and phylogeography of this species on the Balkan peninsula
SP  - 1
EP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3557
ER  - 

@phdthesis{
author = "Budinski, Ivana",
year = "2019",
abstract = "The population genetic structure of a species is a result of past events such as bottlenecks, population expansion and secondary contact, as well as recent events and phenomena like migrations, philopatry, sociality etc. Climatic oscillations during the Pleistocene shaped the present distribution of genetic diversity of temperate biota in Europe. The Balkan peninsula has been recognized as a glacial refugium for many temperate species, including many bat species. The Mediterranean horseshoe bat Rhinolophus euryale is distributed in the Mediterranean area. It is predominantly cave-dwelling species and its distribution greatly overlaps with karstic regions. During summer females form nursery colonies where they give birth and rear pups, while in autumn both sexes gather together into hibernation roosts. Roost fidelity has been found in both sexes. R. euryale is considered to be a sedentary species, since the distance between summer and winter roosts is rarely greater than 50 km. Distribution of this species in Serbia overlaps with karstic areas in western and eastern Serbia. The aims of this thesis were to gather all available data about species’ distribution and to evaluate population trends in Serbia; testing ISSR-PCR method for reliable distinguishing this species from morphologically similar R. blasii. D-loop mitochondrial sequences were used for analyses of genetic diversity, demographic history and phylogeography of the Mediterranean horseshoe bat in the Balkan peninsula. Genetic variability of 12 populations in the central Balkans was analysed using nuclear microsatellites. Genetic differentiation and gene flow among populations from different geographic regions were estimated, and relatedness within different colony types was assessed. In the period from 2012 to 2017, R. euryale was found at 26 localities in Serbia (11 localities for the first time), and now the number of localities counts 73. Several new nursery and hibernation colonies of this species were found, as well as one male summer colony. Only one roost was found in a building, while the rest were in underground sites. No significant threats were identified, and the population trend of this species in Serbia is estimated to be stable. ISSR-PCR represents fast and reliable method for unambiguously distinguishing morphologically similar medium-sized horseshoe species R. euryale and R. blasii. This study represents first population genetic analysis of the Mediterranean horseshoe bat in the central Balkans. High haplotype diversity of mitochondrial D-loop sequences was found, and all samples from Europe, Anatolia and Northern Africa clustered within one phylogenetic clade. Obtained results support the scenario of a sudden demographic expansion, and it is estimated that this started in the late Pleistocene. The Balkan peninsula was probably one of the glacial refugia of this species in Europe. High genetic diversity was also found using nuclear microsatellites. Moderate genetic substructuring among geographic regions and significant isolation by distance were observed. Genetic differentiation among populations from western and eastern Serbia was small but significant, which can be explained by roost fidelity and non-migratory behaviour of the species. The population from Montenegro was genetically similar to ones from eastern Serbia, which implicates the potential existence of colonies between the analysed regions. Kinship is probably not a major factor driving grouping of females into nursery colonies, but, more likely, benefits from group thermoregulation and information transfer about suitable roosts and foraging areas. Information transfer is likely underlying the formation of male summer colonies. High genetic diversity was found among Mediterranean horseshoe bat populations in the central parts of the Balkan peninsula, both for nuclear and mitochondrial markers. These populations harbour significant portion of species’ genetic diversity, and they have been recognized as the most numerous and the most stable across the distribution of R. euryale. Therefore, protecting Balkan populations might play a vital role in conservation of Mediterranean horseshoe bat., Genetička struktura populacija predstavlja rezultat efekta prošlih događaja kao što su prolazak populacija kroz usko grlo, postglacijalna ekspanzija, sekundarni kontakt, ali i recentnih događaja i fenomena koji obuhvataju migracije, filopatriju, socijalnost itd. Pleistocenske klimatske oscilacije uticale su na današnju distribuciju genetičkog diverziteta mnogih vrsta koje naseljavaju Evropu. Balkansko poluostrvo je prepoznato kao glacijalni refugijum za veliki broj vrsta umerenih područja, uključujući i određene vrste slepih miševa. Sredozemni potkovičar Rhinolophus euryale je rasprostranjen na širem području oko Sredozemnog mora. Najčešće koristi podzemna skloništa kao što su pećine, i zbog toga je njegova distribucija vezana za krečnjačke predele. Tokom leta se ženke grupišu u porodiljske kolonije gde se odvija koćenje i podizanje mladih, a jedinke oba pola se u jesen okupljaju u hibernacijske kolonije. Filopatrija je zabeležena kod jedinki oba pola. R. euryale se smatra sedentarnom vrstom jer između letnjih i zimskih skloništa retko prelazi udaljenosti veće od 50 km. U Srbiji se distribucija ove vrste poklapa sa krečnjačkim područjima u zapadnoj i istočnoj Srbiji. Ciljevi ove doktorske disertacije bili su sistematizacija svih podataka o rasprostranjenosti ove vrste i evaluacija populacionih trendova u Srbiji; testiranje ISSR-PCR metode za brzo i pouzdano razlikovanje ove vrste i morfološki slične vrste R. blasii. Sekvence mitohondrijske D-petlje su korišćene za analizu genetičke varijabilnosti, demografske istorije i filogeografije ove vrste na Balkanskom poluostrvu. Pomoću mikrosatelitskih markera analizirana je genetička varijabilnost ove vrste u 12 populacija na području centralnog Balkana, testirana je genetička diferenciranost i procenjen protok gena između geografskih regiona. Pored toga, analiziran je stepen srodstva između jedinki unutar različitih tipova kolonija. U periodu od 2012 do 2017. godine, sredozemni potkovičar je zabeležen na ukupno 26 lokaliteta u Srbiji (na 11 je zabeležen prvi put). Ovime je broj lokaliteta u Srbiji sa dokazanim prisustvom istraživane vrste porastao na 73. Pronađeno je nekoliko novih porodiljskih i hibernacijskih kolonija, kao i jedna letnja kolonija mužjaka. Zabeleženo je samo jedno sklonište u građevini, dok su sva ostala bila u podzemnim objektima. Nisu identifikovani značajni faktori ugrožavanja, i procenjeno je da ova vrsta u Srbiji ima stabilan populacioni trend. Upotrebom ISSR-PCR markera je moguće brzo i sa velikom pouzdanošću razlikovati morfološki slične vrste srednjih potkovičara R. euryale i R. blasii. Ova studija predstavlja prvu populaciono-genetičku analizu sredozemnog potkovičara na području centralnog Balkana. Zabeležen je veliki diverzitet haplotipova D-petlje mitohondrijske DNK, i svi uzorci sa područja Evrope, Anadolije i Severne Afrike su se grupisali u jednu filogenetsku kladu. Dobijeni rezultati podržavaju scenario brze demografske ekspanzije, a procenjeno je da je ekspanzija počela krajem Pleistocena. Balkansko poluostrvo je najverovatnije predstavljalo jedan od refugijuma ove vrste u Evropi. Upotrebom mikrosatelitskih markera je takođe zabeležen veliki genetički diverzitet. Na analiziranom području postoji umereni nivo diferencijacije između geografskih regiona, i značajna izolacija putem distance. Genetička diferenciranost između populacija u zapadnoj i istočnoj Srbiji je bila mala ali značajna, što se može objasniti sedentarnošću i filopatrijom vrste. Populacija iz Crne Gore je genetički slična populacijama iz istočne Srbije, što ukazuje na potencijalno postojanje kolonija između tih regiona. Pokazano je da je stepen srodstva između jedinki unutar različitih tipova kolonija sličan. Mehanizmi koji leže u osnovi formiranja porodiljskih kolonija su najverovatnije korist od grupne termoregulacije i prenos informacija o adekvatnim skloništima i lovnim teritorijama. Razmena informacija je takođe verovatno jedan od razloga grupisanja mužjaka u letnje kolonije. U Balkanskim populacijama sredozemnog potkovičara zabeležen je veliki genetički diverzitet, kako na nuklearnom, tako i na mitohondrijskom nivou. Ove populacije sadrže značajni deo genetičkog diverziteta vrste, i prepoznate su kao najbrojnije i najstabilnije na celom arealu R. euryale. Očuvanje i zaštita balkanskih populacija sredozemnog potkovičara mogu imati krucijalni značaj u opstanku ove vrste.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "University of Belgrade, Faculty of Biology",
title = "Genetička struktura populacija sredozemnog potkovičara Rhinolophus euryale Blasius, 1853 u Srbiji i filogeografija ove vrste na Balkanskom poluostrvu, Population genetic structure of the Mediterranean horseshoe bat Rhinolophus euryale Blasius, 1853 in Serbia and phylogeography of this species on the Balkan peninsula",
pages = "1-96",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3557"
}

Budinski, I.. (2019). Genetička struktura populacija sredozemnog potkovičara Rhinolophus euryale Blasius, 1853 u Srbiji i filogeografija ove vrste na Balkanskom poluostrvu. in University of Belgrade, Faculty of Biology
Belgrade: University of Belgrade, Faculty of Biology., 1-96.
https://hdl.handle.net/21.15107/rcub_ibiss_3557

Budinski I. Genetička struktura populacija sredozemnog potkovičara Rhinolophus euryale Blasius, 1853 u Srbiji i filogeografija ove vrste na Balkanskom poluostrvu. in University of Belgrade, Faculty of Biology. 2019;:1-96.
https://hdl.handle.net/21.15107/rcub_ibiss_3557 .

Budinski, Ivana, "Genetička struktura populacija sredozemnog potkovičara Rhinolophus euryale Blasius, 1853 u Srbiji i filogeografija ove vrste na Balkanskom poluostrvu" in University of Belgrade, Faculty of Biology (2019):1-96,
https://hdl.handle.net/21.15107/rcub_ibiss_3557 .

TY  - JOUR
AU  - Ilić, Marija
AU  - Jojić, Vida
AU  - Stamenković, Gorana
AU  - Marković, Vanja
AU  - Simić, Vladica
AU  - Paunović, Momir
AU  - Crnobrnja-Isailović, Jelka
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3383
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3515
AB  - We conducted a comparative (2D landmark-based geometric and traditional) morphometric analysis on tadpoles at early developmental stages. Two species of brown frog (Rana dalmatina and R. temporaria) and the common toad (Bufo bufo) were involved, all raised in the laboratory from fertilized eggs collected in their natural habitat. Taxonomic identification was confirmed by the DNA barcoding method with the 16S rRNA sequence as the gene marker. Interested to compare the methodologies for quantification and description of morphological differences among tadpoles of mentioned species, we aimed to: 1) calculate interspecies genetic distances as the most relevant measurement for species differentiation, 2) determine and describe size and shape variation, 3) identify relationships among the analyzed species at the morphological level and 4) assess their classification accuracy. Within the framework of the specified aims, both methodologies produced very similar results, i.e., the smallest divergence was between R. dalmatina and R. temporaria, while the most discriminative were B. bufo and R. temporaria. However, we observed subtle shape variation of the distal region of the tail that was detected only by the geometric morphometrics. Our findings support the following. Geometric morphometric method captures more subtle shape differences that were unable to be recovered from linear measurements. It performs slightly better in classification rate. Although it was not quantified, it stands to reason that there is no difference in time investment between the two approaches. Geometric morphometrics provides more information that can be leveraged to answer further questions and it has a clear advantage in visualizing.
T2  - Amphibia-Reptilia
T1  - Geometric vs. traditional morphometric methods for exploring morphological variation of tadpoles at early developmental stages
IS  - 4
VL  - 40
DO  - 10.1163/15685381-00001193
SP  - 499
EP  - 509
ER  - 

@article{
author = "Ilić, Marija and Jojić, Vida and Stamenković, Gorana and Marković, Vanja and Simić, Vladica and Paunović, Momir and Crnobrnja-Isailović, Jelka",
year = "2019",
abstract = "We conducted a comparative (2D landmark-based geometric and traditional) morphometric analysis on tadpoles at early developmental stages. Two species of brown frog (Rana dalmatina and R. temporaria) and the common toad (Bufo bufo) were involved, all raised in the laboratory from fertilized eggs collected in their natural habitat. Taxonomic identification was confirmed by the DNA barcoding method with the 16S rRNA sequence as the gene marker. Interested to compare the methodologies for quantification and description of morphological differences among tadpoles of mentioned species, we aimed to: 1) calculate interspecies genetic distances as the most relevant measurement for species differentiation, 2) determine and describe size and shape variation, 3) identify relationships among the analyzed species at the morphological level and 4) assess their classification accuracy. Within the framework of the specified aims, both methodologies produced very similar results, i.e., the smallest divergence was between R. dalmatina and R. temporaria, while the most discriminative were B. bufo and R. temporaria. However, we observed subtle shape variation of the distal region of the tail that was detected only by the geometric morphometrics. Our findings support the following. Geometric morphometric method captures more subtle shape differences that were unable to be recovered from linear measurements. It performs slightly better in classification rate. Although it was not quantified, it stands to reason that there is no difference in time investment between the two approaches. Geometric morphometrics provides more information that can be leveraged to answer further questions and it has a clear advantage in visualizing.",
journal = "Amphibia-Reptilia",
title = "Geometric vs. traditional morphometric methods for exploring morphological variation of tadpoles at early developmental stages",
number = "4",
volume = "40",
doi = "10.1163/15685381-00001193",
pages = "499-509"
}

Ilić, M., Jojić, V., Stamenković, G., Marković, V., Simić, V., Paunović, M.,& Crnobrnja-Isailović, J.. (2019). Geometric vs. traditional morphometric methods for exploring morphological variation of tadpoles at early developmental stages. in Amphibia-Reptilia, 40(4), 499-509.
https://doi.org/10.1163/15685381-00001193

Ilić M, Jojić V, Stamenković G, Marković V, Simić V, Paunović M, Crnobrnja-Isailović J. Geometric vs. traditional morphometric methods for exploring morphological variation of tadpoles at early developmental stages. in Amphibia-Reptilia. 2019;40(4):499-509.
doi:10.1163/15685381-00001193 .

Ilić, Marija, Jojić, Vida, Stamenković, Gorana, Marković, Vanja, Simić, Vladica, Paunović, Momir, Crnobrnja-Isailović, Jelka, "Geometric vs. traditional morphometric methods for exploring morphological variation of tadpoles at early developmental stages" in Amphibia-Reptilia, 40, no. 4 (2019):499-509,
https://doi.org/10.1163/15685381-00001193 . .

TY  - JOUR
AU  - Ilić, Marija
AU  - Jojić, Vida
AU  - Stamenković, Gorana
AU  - Marković, Vanja
AU  - Simić, Vladica
AU  - Paunović, Momir
AU  - Crnobrnja-Isailović, Jelka
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3383
AB  - We conducted a comparative (2D landmark-based geometric and traditional) morphometric analysis on tadpoles at early developmental stages. Two species of brown frog (Rana dalmatina and R. temporaria) and the common toad (Bufo bufo) were involved, all raised in the laboratory from fertilized eggs collected in their natural habitat. Taxonomic identification was confirmed by the DNA barcoding method with the 16S rRNA sequence as the gene marker. Interested to compare the methodologies for quantification and description of morphological differences among tadpoles of mentioned species, we aimed to: 1) calculate interspecies genetic distances as the most relevant measurement for species differentiation, 2) determine and describe size and shape variation, 3) identify relationships among the analyzed species at the morphological level and 4) assess their classification accuracy. Within the framework of the specified aims, both methodologies produced very similar results, i.e., the smallest divergence was between R. dalmatina and R. temporaria, while the most discriminative were B. bufo and R. temporaria. However, we observed subtle shape variation of the distal region of the tail that was detected only by the geometric morphometrics. Our findings support the following. Geometric morphometric method captures more subtle shape differences that were unable to be recovered from linear measurements. It performs slightly better in classification rate. Although it was not quantified, it stands to reason that there is no difference in time investment between the two approaches. Geometric morphometrics provides more information that can be leveraged to answer further questions and it has a clear advantage in visualizing.
T2  - Amphibia-Reptilia
T1  - Geometric vs. traditional morphometric methods for exploring morphological variation of tadpoles at early developmental stages
IS  - 4
VL  - 40
DO  - 10.1163/15685381-00001193
SP  - 499
EP  - 509
ER  - 

@article{
author = "Ilić, Marija and Jojić, Vida and Stamenković, Gorana and Marković, Vanja and Simić, Vladica and Paunović, Momir and Crnobrnja-Isailović, Jelka",
year = "2019",
abstract = "We conducted a comparative (2D landmark-based geometric and traditional) morphometric analysis on tadpoles at early developmental stages. Two species of brown frog (Rana dalmatina and R. temporaria) and the common toad (Bufo bufo) were involved, all raised in the laboratory from fertilized eggs collected in their natural habitat. Taxonomic identification was confirmed by the DNA barcoding method with the 16S rRNA sequence as the gene marker. Interested to compare the methodologies for quantification and description of morphological differences among tadpoles of mentioned species, we aimed to: 1) calculate interspecies genetic distances as the most relevant measurement for species differentiation, 2) determine and describe size and shape variation, 3) identify relationships among the analyzed species at the morphological level and 4) assess their classification accuracy. Within the framework of the specified aims, both methodologies produced very similar results, i.e., the smallest divergence was between R. dalmatina and R. temporaria, while the most discriminative were B. bufo and R. temporaria. However, we observed subtle shape variation of the distal region of the tail that was detected only by the geometric morphometrics. Our findings support the following. Geometric morphometric method captures more subtle shape differences that were unable to be recovered from linear measurements. It performs slightly better in classification rate. Although it was not quantified, it stands to reason that there is no difference in time investment between the two approaches. Geometric morphometrics provides more information that can be leveraged to answer further questions and it has a clear advantage in visualizing.",
journal = "Amphibia-Reptilia",
title = "Geometric vs. traditional morphometric methods for exploring morphological variation of tadpoles at early developmental stages",
number = "4",
volume = "40",
doi = "10.1163/15685381-00001193",
pages = "499-509"
}

Ilić, M., Jojić, V., Stamenković, G., Marković, V., Simić, V., Paunović, M.,& Crnobrnja-Isailović, J.. (2019). Geometric vs. traditional morphometric methods for exploring morphological variation of tadpoles at early developmental stages. in Amphibia-Reptilia, 40(4), 499-509.
https://doi.org/10.1163/15685381-00001193

Ilić M, Jojić V, Stamenković G, Marković V, Simić V, Paunović M, Crnobrnja-Isailović J. Geometric vs. traditional morphometric methods for exploring morphological variation of tadpoles at early developmental stages. in Amphibia-Reptilia. 2019;40(4):499-509.
doi:10.1163/15685381-00001193 .

Ilić, Marija, Jojić, Vida, Stamenković, Gorana, Marković, Vanja, Simić, Vladica, Paunović, Momir, Crnobrnja-Isailović, Jelka, "Geometric vs. traditional morphometric methods for exploring morphological variation of tadpoles at early developmental stages" in Amphibia-Reptilia, 40, no. 4 (2019):499-509,
https://doi.org/10.1163/15685381-00001193 . .

TY  - JOUR
AU  - Blagojević, Jelena
AU  - Rajičić, Marija
AU  - Jovanović, Vladimir
AU  - Adnađević, Tanja
AU  - Budinski, Ivana
AU  - Bajić, Branka
AU  - Vujošević, Mladen
PY  - 2018
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641800004B
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/2364
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3283
AB  - Arvicoline voles are known as a karyotypically extremely polymorphic group in which the genus Microtus leads with the highest rate of karyotype change. A member of this genus, the European pine vole Microtus subterraneus (de Selys-Longchamps, 1836), is widely distributed in Europe and parts of Asia. There are two cytotypes differing in diploid chromosome number, 2n=54 and 52, each showing different chromosomal polymorphisms. At two localities in southeastern Serbia, Mt. Jastrebac and Vlasina, we found the 2n=52 cytotype. Meiotic preparations from males revealed the presence of asynaptic sex chromosomes. Although asynaptic sex chromosomes are frequent in Microtus, this is the first finding for M. subterraneus. From summarized data it appears that two-thirds of the studied species, mainly from Microtus and Terricola subgenera, possess asynaptic sex chromosomes.
T2  - Archives of Biological Sciences
T1  - One more Microtus species with asynaptic sex chromosomes
IS  - 3
VL  - 70
DO  - 10.2298/ABS171113004B
SP  - 443
EP  - 447
ER  - 

@article{
author = "Blagojević, Jelena and Rajičić, Marija and Jovanović, Vladimir and Adnađević, Tanja and Budinski, Ivana and Bajić, Branka and Vujošević, Mladen",
year = "2018",
abstract = "Arvicoline voles are known as a karyotypically extremely polymorphic group in which the genus Microtus leads with the highest rate of karyotype change. A member of this genus, the European pine vole Microtus subterraneus (de Selys-Longchamps, 1836), is widely distributed in Europe and parts of Asia. There are two cytotypes differing in diploid chromosome number, 2n=54 and 52, each showing different chromosomal polymorphisms. At two localities in southeastern Serbia, Mt. Jastrebac and Vlasina, we found the 2n=52 cytotype. Meiotic preparations from males revealed the presence of asynaptic sex chromosomes. Although asynaptic sex chromosomes are frequent in Microtus, this is the first finding for M. subterraneus. From summarized data it appears that two-thirds of the studied species, mainly from Microtus and Terricola subgenera, possess asynaptic sex chromosomes.",
journal = "Archives of Biological Sciences",
title = "One more Microtus species with asynaptic sex chromosomes",
number = "3",
volume = "70",
doi = "10.2298/ABS171113004B",
pages = "443-447"
}

Blagojević, J., Rajičić, M., Jovanović, V., Adnađević, T., Budinski, I., Bajić, B.,& Vujošević, M.. (2018). One more Microtus species with asynaptic sex chromosomes. in Archives of Biological Sciences, 70(3), 443-447.
https://doi.org/10.2298/ABS171113004B

Blagojević J, Rajičić M, Jovanović V, Adnađević T, Budinski I, Bajić B, Vujošević M. One more Microtus species with asynaptic sex chromosomes. in Archives of Biological Sciences. 2018;70(3):443-447.
doi:10.2298/ABS171113004B .

Blagojević, Jelena, Rajičić, Marija, Jovanović, Vladimir, Adnađević, Tanja, Budinski, Ivana, Bajić, Branka, Vujošević, Mladen, "One more Microtus species with asynaptic sex chromosomes" in Archives of Biological Sciences, 70, no. 3 (2018):443-447,
https://doi.org/10.2298/ABS171113004B . .

TY  - JOUR
AU  - Jovanović, Vladimir
AU  - Čabrilo, Borislav
AU  - Budinski, Ivana
AU  - Bjelić-Čabrilo, Olivera
AU  - Adnađević, Tanja
AU  - Blagojević, Jelena
AU  - Vujošević, Mladen
PY  - 2018
UR  - https://www.cambridge.org/core/product/identifier/S0022149X18000548/type/journal_article
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3111
AB  - The yellow-necked mouse, Apodemus flavicollis, can be considered as a model for genetic polymorphism produced by the frequent presence of supernumerary or B chromosomes (Bs). Host genetic background is rarely taken into account in studies of parasite sex ratio. The main aim of this study was to investigate the range of infrapopulation sex ratios for nematode parasites of the yellow-necked mouse and to determine which factors most influence variation in parasite sex ratios. Six nematode species found in the collected yellow-necked mice were analysed. We confirmed the predominant pattern of female-biased sex ratios in vertebrate parasite infrapopulations. The presence of B chromosomes in host genomes played an important role in infrapopulations of Heligmosomoides polygyrus, Syphacia stroma and Trichuris muris, as hosts with B chromosomes carried a higher proportion of males. The relative increase of males in infrapopulations could result from a shift in parasite life history strategy, induced by adaptation to the specific host genotypes (Bs present). In a meta-analysis with previously published data, the sex determination system was demonstrated to play a significant role in nematode sex ratio variation, as well as specific life history patterns, such as the place of egg hatching.
T2  - Journal of Helminthology
T1  - Host B chromosomes as potential sex ratio distorters of intestinal nematode infrapopulations in the yellow-necked mouse (Apodemus flavicollis).
DO  - 10.1017/S0022149X18000548
ER  - 

@article{
author = "Jovanović, Vladimir and Čabrilo, Borislav and Budinski, Ivana and Bjelić-Čabrilo, Olivera and Adnađević, Tanja and Blagojević, Jelena and Vujošević, Mladen",
year = "2018",
abstract = "The yellow-necked mouse, Apodemus flavicollis, can be considered as a model for genetic polymorphism produced by the frequent presence of supernumerary or B chromosomes (Bs). Host genetic background is rarely taken into account in studies of parasite sex ratio. The main aim of this study was to investigate the range of infrapopulation sex ratios for nematode parasites of the yellow-necked mouse and to determine which factors most influence variation in parasite sex ratios. Six nematode species found in the collected yellow-necked mice were analysed. We confirmed the predominant pattern of female-biased sex ratios in vertebrate parasite infrapopulations. The presence of B chromosomes in host genomes played an important role in infrapopulations of Heligmosomoides polygyrus, Syphacia stroma and Trichuris muris, as hosts with B chromosomes carried a higher proportion of males. The relative increase of males in infrapopulations could result from a shift in parasite life history strategy, induced by adaptation to the specific host genotypes (Bs present). In a meta-analysis with previously published data, the sex determination system was demonstrated to play a significant role in nematode sex ratio variation, as well as specific life history patterns, such as the place of egg hatching.",
journal = "Journal of Helminthology",
title = "Host B chromosomes as potential sex ratio distorters of intestinal nematode infrapopulations in the yellow-necked mouse (Apodemus flavicollis).",
doi = "10.1017/S0022149X18000548"
}

Jovanović, V., Čabrilo, B., Budinski, I., Bjelić-Čabrilo, O., Adnađević, T., Blagojević, J.,& Vujošević, M.. (2018). Host B chromosomes as potential sex ratio distorters of intestinal nematode infrapopulations in the yellow-necked mouse (Apodemus flavicollis).. in Journal of Helminthology.
https://doi.org/10.1017/S0022149X18000548

Jovanović V, Čabrilo B, Budinski I, Bjelić-Čabrilo O, Adnađević T, Blagojević J, Vujošević M. Host B chromosomes as potential sex ratio distorters of intestinal nematode infrapopulations in the yellow-necked mouse (Apodemus flavicollis).. in Journal of Helminthology. 2018;.
doi:10.1017/S0022149X18000548 .

Jovanović, Vladimir, Čabrilo, Borislav, Budinski, Ivana, Bjelić-Čabrilo, Olivera, Adnađević, Tanja, Blagojević, Jelena, Vujošević, Mladen, "Host B chromosomes as potential sex ratio distorters of intestinal nematode infrapopulations in the yellow-necked mouse (Apodemus flavicollis)." in Journal of Helminthology (2018),
https://doi.org/10.1017/S0022149X18000548 . .

TY  - JOUR
AU  - Makunin, Alexey I.
AU  - Rajičić, Marija
AU  - Karamysheva, Tatyana V.
AU  - Romanenko, Svetlana A.
AU  - Druzhkova, Anna S.
AU  - Blagojević, Jelena
AU  - Vujošević, Mladen
AU  - Rubtsov, Nikolay B.
AU  - Graphodatsky, Alexander S.
AU  - Trifonov, Vladimir A.
PY  - 2018
UR  - http://link.springer.com/10.1007/s00412-018-0662-0
UR  - http://www.ncbi.nlm.nih.gov/pubmed/29380046
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2969
AB  - Supernumerary chromosomes sporadically arise in many eukaryotic species as a result of genomic rearrangements. If present in a substantial part of species population, those are called B chromosomes, or Bs. This is the case for 70 mammalian species, most of which are rodents. In humans, the most common types of extra chromosomes, sSMCs (small supernumerary marker chromosomes), are diagnosed in approximately 1 of 2000 postnatal cases. Due to low frequency in population, human sSMCs are not considered B chromosomes. Genetic content of both B-chromosomes and sSMCs in most cases remains understudied. Here, we apply microdissection of single chromosomes with subsequent low-pass sequencing on Ion Torrent PGM and Illumina MiSeq to identify unique and repetitive DNA sequences present in a single human sSMC and several B chromosomes in mice Apodemus flavicollis and Apodemus peninsulae. The pipeline for sequencing data analysis was made available in Galaxy interface as an addition to previously published command-line version. Human sSMC was attributed to the proximal part of chromosome 15 long arm, and breakpoints leading to its formation were located into satellite DNA arrays. Genetic content of Apodemus B chromosomes was species-specific, and minor alterations were observed in both species. Common features of Bs in these Apodemus species were satellite DNA and ERV enrichment, as well as the presence of the vaccinia-related kinase gene Vrk1. Understanding of the non-essential genome elements content provides important insights into genome evolution in general.
T2  - Chromosoma
T1  - Low-pass single-chromosome sequencing of human small supernumerary marker chromosomes (sSMCs) and Apodemus B chromosomes.
IS  - 3
VL  - 127
DO  - 10.1007/s00412-018-0662-0
SP  - 301
EP  - 311
ER  - 

@article{
author = "Makunin, Alexey I. and Rajičić, Marija and Karamysheva, Tatyana V. and Romanenko, Svetlana A. and Druzhkova, Anna S. and Blagojević, Jelena and Vujošević, Mladen and Rubtsov, Nikolay B. and Graphodatsky, Alexander S. and Trifonov, Vladimir A.",
year = "2018",
abstract = "Supernumerary chromosomes sporadically arise in many eukaryotic species as a result of genomic rearrangements. If present in a substantial part of species population, those are called B chromosomes, or Bs. This is the case for 70 mammalian species, most of which are rodents. In humans, the most common types of extra chromosomes, sSMCs (small supernumerary marker chromosomes), are diagnosed in approximately 1 of 2000 postnatal cases. Due to low frequency in population, human sSMCs are not considered B chromosomes. Genetic content of both B-chromosomes and sSMCs in most cases remains understudied. Here, we apply microdissection of single chromosomes with subsequent low-pass sequencing on Ion Torrent PGM and Illumina MiSeq to identify unique and repetitive DNA sequences present in a single human sSMC and several B chromosomes in mice Apodemus flavicollis and Apodemus peninsulae. The pipeline for sequencing data analysis was made available in Galaxy interface as an addition to previously published command-line version. Human sSMC was attributed to the proximal part of chromosome 15 long arm, and breakpoints leading to its formation were located into satellite DNA arrays. Genetic content of Apodemus B chromosomes was species-specific, and minor alterations were observed in both species. Common features of Bs in these Apodemus species were satellite DNA and ERV enrichment, as well as the presence of the vaccinia-related kinase gene Vrk1. Understanding of the non-essential genome elements content provides important insights into genome evolution in general.",
journal = "Chromosoma",
title = "Low-pass single-chromosome sequencing of human small supernumerary marker chromosomes (sSMCs) and Apodemus B chromosomes.",
number = "3",
volume = "127",
doi = "10.1007/s00412-018-0662-0",
pages = "301-311"
}

Makunin, A. I., Rajičić, M., Karamysheva, T. V., Romanenko, S. A., Druzhkova, A. S., Blagojević, J., Vujošević, M., Rubtsov, N. B., Graphodatsky, A. S.,& Trifonov, V. A.. (2018). Low-pass single-chromosome sequencing of human small supernumerary marker chromosomes (sSMCs) and Apodemus B chromosomes.. in Chromosoma, 127(3), 301-311.
https://doi.org/10.1007/s00412-018-0662-0

Makunin AI, Rajičić M, Karamysheva TV, Romanenko SA, Druzhkova AS, Blagojević J, Vujošević M, Rubtsov NB, Graphodatsky AS, Trifonov VA. Low-pass single-chromosome sequencing of human small supernumerary marker chromosomes (sSMCs) and Apodemus B chromosomes.. in Chromosoma. 2018;127(3):301-311.
doi:10.1007/s00412-018-0662-0 .

Makunin, Alexey I., Rajičić, Marija, Karamysheva, Tatyana V., Romanenko, Svetlana A., Druzhkova, Anna S., Blagojević, Jelena, Vujošević, Mladen, Rubtsov, Nikolay B., Graphodatsky, Alexander S., Trifonov, Vladimir A., "Low-pass single-chromosome sequencing of human small supernumerary marker chromosomes (sSMCs) and Apodemus B chromosomes." in Chromosoma, 127, no. 3 (2018):301-311,
https://doi.org/10.1007/s00412-018-0662-0 . .

TY  - JOUR
AU  - Kemenesi, Gábor
AU  - Kurucz, Kornélia
AU  - Zana, Brigitta
AU  - Földes, Fanni
AU  - Urbán, Péter
AU  - Vlaschenko, Anton
AU  - Kravchenko, Kseniia
AU  - Budinski, Ivana
AU  - Szodoray-Parádi, Farkas
AU  - Bücs, Szilárd
AU  - Jére, Csaba
AU  - Csősz, István
AU  - Szodoray-Parádi, Abigél
AU  - Estók, Péter
AU  - Görföl, Tamás
AU  - Boldogh, Sándor
AU  - Jakab, Ferenc
PY  - 2018
UR  - http://link.springer.com/10.1007/s00705-017-3678-5
UR  - http://www.ncbi.nlm.nih.gov/pubmed/29247338
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2947
AB  - Circular replication-associated protein encoding single-stranded DNA (CRESS DNA) viruses are increasingly recognized worldwide in a variety of samples. Representative members include well-described veterinary pathogens with worldwide distribution, such as porcine circoviruses or beak and feather disease virus. In addition, numerous novel viruses belonging to the family Circoviridae with unverified pathogenic roles have been discovered in different human samples. Viruses of the family Genomoviridae have also been described as being highly abundant in different faecal and environmental samples, with case reports showing them to be suspected pathogens in human infections. In order to investigate the genetic diversity of these viruses in European bat populations, we tested guano samples from Georgia, Hungary, Romania, Serbia and Ukraine. This resulted in the detection of six novel members of the family Circoviridae and two novel members of the family Genomoviridae. Interestingly, a gemini-like virus, namely niminivirus, which was originally found in raw sewage samples in Nigeria, was also detected in our samples. We analyzed the nucleotide composition of members of the family Circoviridae to determine the possible host origins of these viruses. This study provides the first dataset on CRESS DNA viruses of European bats, and members of several novel viral species were discovered.
T2  - Archives of Virology
T1  - Diverse replication-associated protein encoding circular DNA viruses in guano samples of Central-Eastern European bats.
IS  - 3
VL  - 163
DO  - 10.1007/s00705-017-3678-5
SP  - 671
EP  - 678
ER  - 

@article{
author = "Kemenesi, Gábor and Kurucz, Kornélia and Zana, Brigitta and Földes, Fanni and Urbán, Péter and Vlaschenko, Anton and Kravchenko, Kseniia and Budinski, Ivana and Szodoray-Parádi, Farkas and Bücs, Szilárd and Jére, Csaba and Csősz, István and Szodoray-Parádi, Abigél and Estók, Péter and Görföl, Tamás and Boldogh, Sándor and Jakab, Ferenc",
year = "2018",
abstract = "Circular replication-associated protein encoding single-stranded DNA (CRESS DNA) viruses are increasingly recognized worldwide in a variety of samples. Representative members include well-described veterinary pathogens with worldwide distribution, such as porcine circoviruses or beak and feather disease virus. In addition, numerous novel viruses belonging to the family Circoviridae with unverified pathogenic roles have been discovered in different human samples. Viruses of the family Genomoviridae have also been described as being highly abundant in different faecal and environmental samples, with case reports showing them to be suspected pathogens in human infections. In order to investigate the genetic diversity of these viruses in European bat populations, we tested guano samples from Georgia, Hungary, Romania, Serbia and Ukraine. This resulted in the detection of six novel members of the family Circoviridae and two novel members of the family Genomoviridae. Interestingly, a gemini-like virus, namely niminivirus, which was originally found in raw sewage samples in Nigeria, was also detected in our samples. We analyzed the nucleotide composition of members of the family Circoviridae to determine the possible host origins of these viruses. This study provides the first dataset on CRESS DNA viruses of European bats, and members of several novel viral species were discovered.",
journal = "Archives of Virology",
title = "Diverse replication-associated protein encoding circular DNA viruses in guano samples of Central-Eastern European bats.",
number = "3",
volume = "163",
doi = "10.1007/s00705-017-3678-5",
pages = "671-678"
}

Kemenesi, G., Kurucz, K., Zana, B., Földes, F., Urbán, P., Vlaschenko, A., Kravchenko, K., Budinski, I., Szodoray-Parádi, F., Bücs, S., Jére, C., Csősz, I., Szodoray-Parádi, A., Estók, P., Görföl, T., Boldogh, S.,& Jakab, F.. (2018). Diverse replication-associated protein encoding circular DNA viruses in guano samples of Central-Eastern European bats.. in Archives of Virology, 163(3), 671-678.
https://doi.org/10.1007/s00705-017-3678-5

Kemenesi G, Kurucz K, Zana B, Földes F, Urbán P, Vlaschenko A, Kravchenko K, Budinski I, Szodoray-Parádi F, Bücs S, Jére C, Csősz I, Szodoray-Parádi A, Estók P, Görföl T, Boldogh S, Jakab F. Diverse replication-associated protein encoding circular DNA viruses in guano samples of Central-Eastern European bats.. in Archives of Virology. 2018;163(3):671-678.
doi:10.1007/s00705-017-3678-5 .

Kemenesi, Gábor, Kurucz, Kornélia, Zana, Brigitta, Földes, Fanni, Urbán, Péter, Vlaschenko, Anton, Kravchenko, Kseniia, Budinski, Ivana, Szodoray-Parádi, Farkas, Bücs, Szilárd, Jére, Csaba, Csősz, István, Szodoray-Parádi, Abigél, Estók, Péter, Görföl, Tamás, Boldogh, Sándor, Jakab, Ferenc, "Diverse replication-associated protein encoding circular DNA viruses in guano samples of Central-Eastern European bats." in Archives of Virology, 163, no. 3 (2018):671-678,
https://doi.org/10.1007/s00705-017-3678-5 . .

TY  - JOUR
AU  - Vujošević, Mladen
AU  - Rajičić, Marija
AU  - Blagojević, Jelena
PY  - 2018
UR  - http://www.mdpi.com/2073-4425/9/10/487
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3172
AB  - The study of B chromosomes (Bs) started more than a century ago, while their presence in mammals dates since 1965. As the past two decades have seen huge progress in application of molecular techniques, we decided to throw a glance on new data on Bs in mammals and to review them. We listed 85 mammals with Bs that make 1.94% of karyotypically studied species. Contrary to general view, a typical B chromosome in mammals appears both as sub- or metacentric that is the same size as small chromosomes of standard complement. Both karyotypically stable and unstable species possess Bs. The presence of Bs in certain species influences the cell division, the degree of recombination, the development, a number of quantitative characteristics, the host-parasite interactions and their behaviour. There is at least some data on molecular structure of Bs recorded in nearly a quarter of species. Nevertheless, a more detailed molecular composition of Bs presently known for six mammalian species, confirms the presence of protein coding genes, and the transcriptional activity for some of them. Therefore, the idea that Bs are inert is outdated, but the role of Bs is yet to be determined. The maintenance of Bs is obviously not the same for all species, so the current models must be adapted while bearing in mind that Bs are not inactive as it was once thought.
T2  - Genes
T1  - B Chromosomes in Populations of Mammals Revisited
IS  - 10
VL  - 9
DO  - 10.3390/genes9100487
SP  - 487
ER  - 

@article{
author = "Vujošević, Mladen and Rajičić, Marija and Blagojević, Jelena",
year = "2018",
abstract = "The study of B chromosomes (Bs) started more than a century ago, while their presence in mammals dates since 1965. As the past two decades have seen huge progress in application of molecular techniques, we decided to throw a glance on new data on Bs in mammals and to review them. We listed 85 mammals with Bs that make 1.94% of karyotypically studied species. Contrary to general view, a typical B chromosome in mammals appears both as sub- or metacentric that is the same size as small chromosomes of standard complement. Both karyotypically stable and unstable species possess Bs. The presence of Bs in certain species influences the cell division, the degree of recombination, the development, a number of quantitative characteristics, the host-parasite interactions and their behaviour. There is at least some data on molecular structure of Bs recorded in nearly a quarter of species. Nevertheless, a more detailed molecular composition of Bs presently known for six mammalian species, confirms the presence of protein coding genes, and the transcriptional activity for some of them. Therefore, the idea that Bs are inert is outdated, but the role of Bs is yet to be determined. The maintenance of Bs is obviously not the same for all species, so the current models must be adapted while bearing in mind that Bs are not inactive as it was once thought.",
journal = "Genes",
title = "B Chromosomes in Populations of Mammals Revisited",
number = "10",
volume = "9",
doi = "10.3390/genes9100487",
pages = "487"
}

Vujošević, M., Rajičić, M.,& Blagojević, J.. (2018). B Chromosomes in Populations of Mammals Revisited. in Genes, 9(10), 487.
https://doi.org/10.3390/genes9100487

Vujošević M, Rajičić M, Blagojević J. B Chromosomes in Populations of Mammals Revisited. in Genes. 2018;9(10):487.
doi:10.3390/genes9100487 .

Vujošević, Mladen, Rajičić, Marija, Blagojević, Jelena, "B Chromosomes in Populations of Mammals Revisited" in Genes, 9, no. 10 (2018):487,
https://doi.org/10.3390/genes9100487 . .

TY  - CONF
AU  - Budinski, Ivana
AU  - Blagojević, Jelena
AU  - Jovanović, Vladimir
AU  - Bajić, Branka
AU  - Rajičić, Marija
AU  - Paunović, Milan
AU  - Vujošević, Mladen
PY  - 2017
UR  - http://ebrs2017.eus/
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2944
AB  - Mediterranean horseshoe bat is considered to be mostly sedentary species, with seasonal movements usually shorter than 50 km. Known roosts of Rhinolophus euryale in Serbia are distributed in western and eastern parts of the country, overlapping with karst distribution. All but one roosts recorded in last 50 years were caves. The distance between caves in Western and Eastern Serbia is greater than 150 km, and the environment between them is devoid of caves. Furthermore, there were no recent records of this species from Central Serbia. Bearing in mind the non-migratory nature of this species, our aim was to test for population structuring (isolation by distance pattern) among different geographic regions.
C3  - 14th European Bat Research Symposium
T1  - Population Genetic Structure of Mediterranean Horseshoe Bat Rhinolophus Euryale in Serbia
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_2944
ER  - 

@conference{
author = "Budinski, Ivana and Blagojević, Jelena and Jovanović, Vladimir and Bajić, Branka and Rajičić, Marija and Paunović, Milan and Vujošević, Mladen",
year = "2017",
abstract = "Mediterranean horseshoe bat is considered to be mostly sedentary species, with seasonal movements usually shorter than 50 km. Known roosts of Rhinolophus euryale in Serbia are distributed in western and eastern parts of the country, overlapping with karst distribution. All but one roosts recorded in last 50 years were caves. The distance between caves in Western and Eastern Serbia is greater than 150 km, and the environment between them is devoid of caves. Furthermore, there were no recent records of this species from Central Serbia. Bearing in mind the non-migratory nature of this species, our aim was to test for population structuring (isolation by distance pattern) among different geographic regions.",
journal = "14th European Bat Research Symposium",
title = "Population Genetic Structure of Mediterranean Horseshoe Bat Rhinolophus Euryale in Serbia",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_2944"
}

Budinski, I., Blagojević, J., Jovanović, V., Bajić, B., Rajičić, M., Paunović, M.,& Vujošević, M.. (2017). Population Genetic Structure of Mediterranean Horseshoe Bat Rhinolophus Euryale in Serbia. in 14th European Bat Research Symposium.
https://hdl.handle.net/21.15107/rcub_ibiss_2944

Budinski I, Blagojević J, Jovanović V, Bajić B, Rajičić M, Paunović M, Vujošević M. Population Genetic Structure of Mediterranean Horseshoe Bat Rhinolophus Euryale in Serbia. in 14th European Bat Research Symposium. 2017;.
https://hdl.handle.net/21.15107/rcub_ibiss_2944 .

Budinski, Ivana, Blagojević, Jelena, Jovanović, Vladimir, Bajić, Branka, Rajičić, Marija, Paunović, Milan, Vujošević, Mladen, "Population Genetic Structure of Mediterranean Horseshoe Bat Rhinolophus Euryale in Serbia" in 14th European Bat Research Symposium (2017),
https://hdl.handle.net/21.15107/rcub_ibiss_2944 .

TY  - JOUR
AU  - Živković, Zlata
AU  - Vidović, Biljana
AU  - Jojić, Vida
AU  - Cvrković, Tatjana
AU  - Petanović, Radmila
PY  - 2017
UR  - http://link.springer.com/10.1007/s10493-017-0128-8
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2750
AB  - We examined morphological and genetic differences among Aceria spp. inhabiting six Brassicaceae species in Serbia. Five of them have been already mentioned in the literature as original or alternate hosts of Aceria spp. (Berteroa incana (L.) DC., Capsella bursa-pastoris (L.) Medik., Cardamine hirsuta L., Lepidium draba L. and Sisymbrium orientale L.), whereas Aurinia petraea (Ard.) Schur was registered here for the first time as a host for Aceria sp. Results of morphometric analyses indicated clear differentiation of Aceria spp. from B. incana, L. draba, A. petraea and S. orientale, whereas the other two entities were less diverse and clustered together. Molecular analyses indicated that the average mean divergence over all sequence pairs was 18.3% (11.8–25.9%) and disclosed that the observed mtCOI distances between the six host-associated Aceria entities are large enough to represent differences between species. The Aceria sp. hosted by B. incana was morphologically and genetically most distinctive, whereas discrepancy between phenetic and phylogenetic relationships was found in the position of Aceria sp. hosted by L. draba when compared with those inhabiting C. hirsuta, S. orientale and C. bursa-pastoris.
T2  - Experimental and Applied Acarology
T1  - Phenetic and phylogenetic relationships among Aceria spp. (Acari: Eriophyoidea) inhabiting species within the family Brassicaceae in Serbia
IS  - 4
VL  - 71
DO  - 10.1007/s10493-017-0128-8
SP  - 329
EP  - 343
ER  - 

@article{
author = "Živković, Zlata and Vidović, Biljana and Jojić, Vida and Cvrković, Tatjana and Petanović, Radmila",
year = "2017",
abstract = "We examined morphological and genetic differences among Aceria spp. inhabiting six Brassicaceae species in Serbia. Five of them have been already mentioned in the literature as original or alternate hosts of Aceria spp. (Berteroa incana (L.) DC., Capsella bursa-pastoris (L.) Medik., Cardamine hirsuta L., Lepidium draba L. and Sisymbrium orientale L.), whereas Aurinia petraea (Ard.) Schur was registered here for the first time as a host for Aceria sp. Results of morphometric analyses indicated clear differentiation of Aceria spp. from B. incana, L. draba, A. petraea and S. orientale, whereas the other two entities were less diverse and clustered together. Molecular analyses indicated that the average mean divergence over all sequence pairs was 18.3% (11.8–25.9%) and disclosed that the observed mtCOI distances between the six host-associated Aceria entities are large enough to represent differences between species. The Aceria sp. hosted by B. incana was morphologically and genetically most distinctive, whereas discrepancy between phenetic and phylogenetic relationships was found in the position of Aceria sp. hosted by L. draba when compared with those inhabiting C. hirsuta, S. orientale and C. bursa-pastoris.",
journal = "Experimental and Applied Acarology",
title = "Phenetic and phylogenetic relationships among Aceria spp. (Acari: Eriophyoidea) inhabiting species within the family Brassicaceae in Serbia",
number = "4",
volume = "71",
doi = "10.1007/s10493-017-0128-8",
pages = "329-343"
}

Živković, Z., Vidović, B., Jojić, V., Cvrković, T.,& Petanović, R.. (2017). Phenetic and phylogenetic relationships among Aceria spp. (Acari: Eriophyoidea) inhabiting species within the family Brassicaceae in Serbia. in Experimental and Applied Acarology, 71(4), 329-343.
https://doi.org/10.1007/s10493-017-0128-8

Živković Z, Vidović B, Jojić V, Cvrković T, Petanović R. Phenetic and phylogenetic relationships among Aceria spp. (Acari: Eriophyoidea) inhabiting species within the family Brassicaceae in Serbia. in Experimental and Applied Acarology. 2017;71(4):329-343.
doi:10.1007/s10493-017-0128-8 .

Živković, Zlata, Vidović, Biljana, Jojić, Vida, Cvrković, Tatjana, Petanović, Radmila, "Phenetic and phylogenetic relationships among Aceria spp. (Acari: Eriophyoidea) inhabiting species within the family Brassicaceae in Serbia" in Experimental and Applied Acarology, 71, no. 4 (2017):329-343,
https://doi.org/10.1007/s10493-017-0128-8 . .

TY  - JOUR
AU  - Rajičić, Marija
AU  - Romanenko, Svetlana A.
AU  - Karamysheva, Tatyana V.
AU  - Blagojević, Jelena
AU  - Adnađević, Tanja
AU  - Budinski, Ivana
AU  - Bogdanov, Aleksey S.
AU  - Trifonov, Vladimir A.
AU  - Rubtsov, Nikolay B.
AU  - Vujošević, Mladen
PY  - 2017
UR  - http://dx.plos.org/10.1371/journal.pone.0172704
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2624
AB  - B chromosomes (Bs) are known for more than hundred years but their origin, structure and pattern of evolution are not well understood. In the past few years new methodological approaches, involving isolation of Bs followed by whole DNA amplification, DNA probe generation, and fluorescent in situ hybridization (FISH) or the B chromosome DNA sequencing, has allowed detailed analysis of their origin and molecular structure in different species. In this study we explored the origin of Bs in the yellow-necked wood mouse, Apodemus flavicollis, using generation of microdissected DNA probes followed by FISH on metaphase chromosomes. Bs of A. flavicollis were successfully isolated and DNA was used as the template for B-specific probes for the first time. We revealed homology of DNA derived from the analyzed B chromosomes to the pericentromeric region (PR) of sex chromosomes and subtelomeric region of two pairs of small autosomes, but lower homology to the rest of the Y chromosome. Moreover, all analysed Bs had the same structure regardless of their number per individual or the great geographic distance between examined populations from the Balkan Peninsula (Serbia) and Eastern Europe (south region of Russia and central Belarus). Therefore, it was suggested that B chromosomes in A. flavicollis have a unique common origin from the PR of sex chromosomes, and/or similar evolutionary pattern.
T2  - PloS one
T1  - The origin of B chromosomes in yellow-necked mice (Apodemus flavicollis)—Break rules but keep playing the game
IS  - 3
VL  - 12
DO  - 10.1371/journal.pone.0172704
SP  - e0172704
EP  - e0172704
ER  - 

@article{
editor = "Houben, Andreas",
author = "Rajičić, Marija and Romanenko, Svetlana A. and Karamysheva, Tatyana V. and Blagojević, Jelena and Adnađević, Tanja and Budinski, Ivana and Bogdanov, Aleksey S. and Trifonov, Vladimir A. and Rubtsov, Nikolay B. and Vujošević, Mladen",
year = "2017",
abstract = "B chromosomes (Bs) are known for more than hundred years but their origin, structure and pattern of evolution are not well understood. In the past few years new methodological approaches, involving isolation of Bs followed by whole DNA amplification, DNA probe generation, and fluorescent in situ hybridization (FISH) or the B chromosome DNA sequencing, has allowed detailed analysis of their origin and molecular structure in different species. In this study we explored the origin of Bs in the yellow-necked wood mouse, Apodemus flavicollis, using generation of microdissected DNA probes followed by FISH on metaphase chromosomes. Bs of A. flavicollis were successfully isolated and DNA was used as the template for B-specific probes for the first time. We revealed homology of DNA derived from the analyzed B chromosomes to the pericentromeric region (PR) of sex chromosomes and subtelomeric region of two pairs of small autosomes, but lower homology to the rest of the Y chromosome. Moreover, all analysed Bs had the same structure regardless of their number per individual or the great geographic distance between examined populations from the Balkan Peninsula (Serbia) and Eastern Europe (south region of Russia and central Belarus). Therefore, it was suggested that B chromosomes in A. flavicollis have a unique common origin from the PR of sex chromosomes, and/or similar evolutionary pattern.",
journal = "PloS one",
title = "The origin of B chromosomes in yellow-necked mice (Apodemus flavicollis)—Break rules but keep playing the game",
number = "3",
volume = "12",
doi = "10.1371/journal.pone.0172704",
pages = "e0172704-e0172704"
}

Houben, A., Rajičić, M., Romanenko, S. A., Karamysheva, T. V., Blagojević, J., Adnađević, T., Budinski, I., Bogdanov, A. S., Trifonov, V. A., Rubtsov, N. B.,& Vujošević, M.. (2017). The origin of B chromosomes in yellow-necked mice (Apodemus flavicollis)—Break rules but keep playing the game. in PloS one, 12(3), e0172704-e0172704.
https://doi.org/10.1371/journal.pone.0172704

Houben A, Rajičić M, Romanenko SA, Karamysheva TV, Blagojević J, Adnađević T, Budinski I, Bogdanov AS, Trifonov VA, Rubtsov NB, Vujošević M. The origin of B chromosomes in yellow-necked mice (Apodemus flavicollis)—Break rules but keep playing the game. in PloS one. 2017;12(3):e0172704-e0172704.
doi:10.1371/journal.pone.0172704 .

Houben, Andreas, Rajičić, Marija, Romanenko, Svetlana A., Karamysheva, Tatyana V., Blagojević, Jelena, Adnađević, Tanja, Budinski, Ivana, Bogdanov, Aleksey S., Trifonov, Vladimir A., Rubtsov, Nikolay B., Vujošević, Mladen, "The origin of B chromosomes in yellow-necked mice (Apodemus flavicollis)—Break rules but keep playing the game" in PloS one, 12, no. 3 (2017):e0172704-e0172704,
https://doi.org/10.1371/journal.pone.0172704 . .

TY  - JOUR
AU  - Jojić, Vida
AU  - Porobić, Jelena
AU  - Ćirović, Duško
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S004452311730013X
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2561
AB  - We used geometric morphometric techniques to examine variability in Serbian red fox (Vulpes vulpes) crania. Male crania are about 5% larger than female ones and sexual size dimorphism (SSD) was significant. Also, significant cranial shape differences were found between the sexes. Males are characterized by reduced basicranial, but broader rostral and zygomatic regions. Although a slight and significant allometric effect was detected and larger specimens are smaller in the region of basicranium, cranial sexual shape dimorphism (SShD) pattern is not influenced by SSD. As small to moderate SSD in canid species is a general characteristics of the family, additional studies are needed to determine whether basicranial reduction in males, i.e. its enlargement in females, could be an important feature in characterizing cranial SShD patterns in other Canidae members. While uniform considering size, the shape of Serbian red fox crania varies geographically, as well as depending on proportion of agricultural habitats. In comparison to those from central and eastern Serbia, specimens from the northern Serbian region Vojvodina (with higher proportions of agricultural areas) have more robust crania with shorter snouts and maxillae, larger palatine bones accompanied with anteriorly moved posterior edges of the canine alveolus and laterally expanded zygomatic arches. Encompassing mostly facial and temporal cranial regions these shape changes are probably related to diet differences, although genetic diversification cannot be excluded as a possible contributing factor.
T2  - Zoologischer Anzeiger - A Journal of Comparative Zoology
T1  - Cranial variability of the Serbian red fox
VL  - 267
DO  - 10.1016/j.jcz.2017.02.001
SP  - 41
EP  - 48
ER  - 

@article{
author = "Jojić, Vida and Porobić, Jelena and Ćirović, Duško",
year = "2017",
abstract = "We used geometric morphometric techniques to examine variability in Serbian red fox (Vulpes vulpes) crania. Male crania are about 5% larger than female ones and sexual size dimorphism (SSD) was significant. Also, significant cranial shape differences were found between the sexes. Males are characterized by reduced basicranial, but broader rostral and zygomatic regions. Although a slight and significant allometric effect was detected and larger specimens are smaller in the region of basicranium, cranial sexual shape dimorphism (SShD) pattern is not influenced by SSD. As small to moderate SSD in canid species is a general characteristics of the family, additional studies are needed to determine whether basicranial reduction in males, i.e. its enlargement in females, could be an important feature in characterizing cranial SShD patterns in other Canidae members. While uniform considering size, the shape of Serbian red fox crania varies geographically, as well as depending on proportion of agricultural habitats. In comparison to those from central and eastern Serbia, specimens from the northern Serbian region Vojvodina (with higher proportions of agricultural areas) have more robust crania with shorter snouts and maxillae, larger palatine bones accompanied with anteriorly moved posterior edges of the canine alveolus and laterally expanded zygomatic arches. Encompassing mostly facial and temporal cranial regions these shape changes are probably related to diet differences, although genetic diversification cannot be excluded as a possible contributing factor.",
journal = "Zoologischer Anzeiger - A Journal of Comparative Zoology",
title = "Cranial variability of the Serbian red fox",
volume = "267",
doi = "10.1016/j.jcz.2017.02.001",
pages = "41-48"
}

Jojić, V., Porobić, J.,& Ćirović, D.. (2017). Cranial variability of the Serbian red fox. in Zoologischer Anzeiger - A Journal of Comparative Zoology, 267, 41-48.
https://doi.org/10.1016/j.jcz.2017.02.001

Jojić V, Porobić J, Ćirović D. Cranial variability of the Serbian red fox. in Zoologischer Anzeiger - A Journal of Comparative Zoology. 2017;267:41-48.
doi:10.1016/j.jcz.2017.02.001 .

Jojić, Vida, Porobić, Jelena, Ćirović, Duško, "Cranial variability of the Serbian red fox" in Zoologischer Anzeiger - A Journal of Comparative Zoology, 267 (2017):41-48,
https://doi.org/10.1016/j.jcz.2017.02.001 . .