TY  - JOUR
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Stojković, Biljana
PY  - 2019
UR  - https://www.mdpi.com/2075-4450/10/6/153
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3357
AB  - Effective pest management strategies for a targeted pest species must rely on accurate, reliable and reproducible estimates of population dynamics. Importance of such approaches is even more conspicuous when assessing pest's potential to utilize other stored products. Using an experimental evolution approach, we have focused our attention on a common bean pest, the seed beetle (Acanthoscelides obtectus). We looked into the potential to invade and sustain population growth on two suboptimal host plants (chickpeas and mung beans). Such an approach simulates steps of the host-shift process in storages. By analyzing population dynamics during initial encountering with a new host plant, we detected a population drop for both novel hosts. However, transgenerational development in a novel environment resulted in a constant population growth in chickpeas, but not in mung bean populations. Reversal of chickpea selected populations to original host plant has led to a severe decrease in population parameters due to low viability of immatures, while the opposite trend was detected in mung bean populations. This paper highlights the importance of good practice in estimating population dynamics for economically important species. With special emphasis on storage pest species, we discuss how this approach can be useful for estimating invading potential of pest insects.
T2  - Insects
T1  - Potential for Acanthoscelides obtectus to Adapt to New Hosts Seen in Laboratory Selection Experiments.
IS  - 6
VL  - 10
DO  - 10.3390/insects10060153
SP  - 153
ER  - 

@article{
author = "Savković, Uroš and Đorđević, Mirko and Stojković, Biljana",
year = "2019",
abstract = "Effective pest management strategies for a targeted pest species must rely on accurate, reliable and reproducible estimates of population dynamics. Importance of such approaches is even more conspicuous when assessing pest's potential to utilize other stored products. Using an experimental evolution approach, we have focused our attention on a common bean pest, the seed beetle (Acanthoscelides obtectus). We looked into the potential to invade and sustain population growth on two suboptimal host plants (chickpeas and mung beans). Such an approach simulates steps of the host-shift process in storages. By analyzing population dynamics during initial encountering with a new host plant, we detected a population drop for both novel hosts. However, transgenerational development in a novel environment resulted in a constant population growth in chickpeas, but not in mung bean populations. Reversal of chickpea selected populations to original host plant has led to a severe decrease in population parameters due to low viability of immatures, while the opposite trend was detected in mung bean populations. This paper highlights the importance of good practice in estimating population dynamics for economically important species. With special emphasis on storage pest species, we discuss how this approach can be useful for estimating invading potential of pest insects.",
journal = "Insects",
title = "Potential for Acanthoscelides obtectus to Adapt to New Hosts Seen in Laboratory Selection Experiments.",
number = "6",
volume = "10",
doi = "10.3390/insects10060153",
pages = "153"
}

Savković, U., Đorđević, M.,& Stojković, B.. (2019). Potential for Acanthoscelides obtectus to Adapt to New Hosts Seen in Laboratory Selection Experiments.. in Insects, 10(6), 153.
https://doi.org/10.3390/insects10060153

Savković U, Đorđević M, Stojković B. Potential for Acanthoscelides obtectus to Adapt to New Hosts Seen in Laboratory Selection Experiments.. in Insects. 2019;10(6):153.
doi:10.3390/insects10060153 .

Savković, Uroš, Đorđević, Mirko, Stojković, Biljana, "Potential for Acanthoscelides obtectus to Adapt to New Hosts Seen in Laboratory Selection Experiments." in Insects, 10, no. 6 (2019):153,
https://doi.org/10.3390/insects10060153 . .

TY  - CONF
AU  - Vlajnić, Lea
AU  - Savković, Uroš
AU  - Stojković, Biljana
AU  - Đorđević, Mirko
PY  - 2019
UR  - https://app.oxfordabstracts.com/events/653/program-app/submission/122870
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3456
AB  - Pest species pose one of the biggest threats to global agriculture, economy and human health. Thus, there is ongoing effort to implement pest control techniques that are often costly and detrimental for environment and non-target species. The Trojan Female Technique (TFT) is a novel approach to pest management via control of reproductive output. The goal of the TFT is to use naturally occurring mtDNA mutations which impair male fertility, but have no effects on females, to achieve sustained pest population suppression. Although described in fruit flies, the candidate TFT mutations have not yet been documented in pest species. In order to identify and test possible TFT candidates for biological control of seed beetle Acanthoscelides obtectus, a common pest in legume storages, we expressed four mtDNA haplotypes found in laboratory lines selected for late reproduction/long life alongside nuclear background of wild populations. Analysis of the effect of specific mitotypes on female and male reproductive potential showed significant male-only reduction in fertility in just one mito-nuclear combination. More specifically, the fertility of males carrying MG3a haplotype was reduced for more than 40% relative to MG3a females. This result indicates MG3a as a promising candidate for further development of the TFT in seed beetles.
C3  - 17th Congress of the  European Society for Evolutionary Biology
T1  - Sex-specific effects of candidate Trojan Female Technique haplotype on fertility in pest species Acanthoscelides obtectus
SP  - S16.P5
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3456
ER  - 

@conference{
author = "Vlajnić, Lea and Savković, Uroš and Stojković, Biljana and Đorđević, Mirko",
year = "2019",
abstract = "Pest species pose one of the biggest threats to global agriculture, economy and human health. Thus, there is ongoing effort to implement pest control techniques that are often costly and detrimental for environment and non-target species. The Trojan Female Technique (TFT) is a novel approach to pest management via control of reproductive output. The goal of the TFT is to use naturally occurring mtDNA mutations which impair male fertility, but have no effects on females, to achieve sustained pest population suppression. Although described in fruit flies, the candidate TFT mutations have not yet been documented in pest species. In order to identify and test possible TFT candidates for biological control of seed beetle Acanthoscelides obtectus, a common pest in legume storages, we expressed four mtDNA haplotypes found in laboratory lines selected for late reproduction/long life alongside nuclear background of wild populations. Analysis of the effect of specific mitotypes on female and male reproductive potential showed significant male-only reduction in fertility in just one mito-nuclear combination. More specifically, the fertility of males carrying MG3a haplotype was reduced for more than 40% relative to MG3a females. This result indicates MG3a as a promising candidate for further development of the TFT in seed beetles.",
journal = "17th Congress of the  European Society for Evolutionary Biology",
title = "Sex-specific effects of candidate Trojan Female Technique haplotype on fertility in pest species Acanthoscelides obtectus",
pages = "S16.P5",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3456"
}

Vlajnić, L., Savković, U., Stojković, B.,& Đorđević, M.. (2019). Sex-specific effects of candidate Trojan Female Technique haplotype on fertility in pest species Acanthoscelides obtectus. in 17th Congress of the  European Society for Evolutionary Biology, S16.P5.
https://hdl.handle.net/21.15107/rcub_ibiss_3456

Vlajnić L, Savković U, Stojković B, Đorđević M. Sex-specific effects of candidate Trojan Female Technique haplotype on fertility in pest species Acanthoscelides obtectus. in 17th Congress of the  European Society for Evolutionary Biology. 2019;:S16.P5.
https://hdl.handle.net/21.15107/rcub_ibiss_3456 .

Vlajnić, Lea, Savković, Uroš, Stojković, Biljana, Đorđević, Mirko, "Sex-specific effects of candidate Trojan Female Technique haplotype on fertility in pest species Acanthoscelides obtectus" in 17th Congress of the  European Society for Evolutionary Biology (2019):S16.P5,
https://hdl.handle.net/21.15107/rcub_ibiss_3456 .

TY  - THES
AU  - Budečević, Sanja
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3258
AB  - Analize unutar - i među - individualnihih razlika morfoloških struktura omogućuju otkrivanje obrazaca fenotipskog variranja, kao i ekološko - evolucionih mehanizama koji dovode do njihove divergencije. U ovoj disertaciji utvrđene su komponente fenotipske varijanse oblika funkcionalno različitih cvetnih organa kod entomofilne biljne vrste Iris pumila L., polimorfne u odnosu na boju cveta. Eksperimenti su vršeni na biljkama gajenim u eksperimentalnoj bašti, poreklom iz recipročnih ukrštanja 24 klonalna genotipa. Metodama geometrijske morfometrije utvrđeni su obrasci variranja oblika tri bilateralno simetrična organa: fola, standarda i grane tučka. Kod sva tri organa udeo simetrične komponente u ukupnom variranju oblika bio je najveći, dok je fluktuirajuća asimetrija (FA) dominirala u asimetričnoj komponenti. Iako se smatra da je primarni uzrok FA nestabilnost razvića, naša istraživanja su pokazala da fenotipska plastičnost može takođe da proizvede FA. Naime, replike cvetnih organa sa različitom orijentacijom prema suncu imale su različite vrednosti asimetričnih komponenti varijanse oblika, kao rezultat plastičnosti u odnosu na mikrosredinsku heterogenost ambijentalne svetlosti. Da veličina, oblik i boja cveta predstavljaju vizuelne signale za privlačenje potencijalnih oprašivača potvrdila su poređenja veličine cvetnih organa oprašenih i neoprašenih biljaka. Oprašeni cvetovi su, po pravilu, imali veće organe od neoprašenih, sa izuzetkom grane tučka. Regresione analize su pokazale da je veličina fola bila pod delovanjem pozitivne, a grane tučka negativne direkcione selekcije. Suprotno tome, oblik cvetnih organa se razlikovao u zavisnosti od boje, što ukazuje da je preferencija oprašivača prema specifičnim kombinacijama vizuelnih signala bila važan mehanizam morfološke divergencije reproduktivnih organa I. pumila.
AB  - Analyzes of intra - and inter - individual differences of morphological structures allow the detection of patterns of phenotypic variation, as well as ecological evolutionary mechanisms leading to their divergence. In this dissertation, components of the phenotypic variation of the shape of functionally distinctive flower organs were determined in the entomophilous plant, specific for striking flower color polymorphism, Iris pumila L. Experiments were carried out on plants grown in experimental garden, originating from reciprocal crossing of 24 clonal genotypes. Using the methods of geometric morphometrics patterns of variation of the shape of three bilaterally symmetrical organs (falls, standards and style branches) have been revealed. In all three floral organs, the proportion of the symmetric component in the overall shape variation was the highest, while the fluctuating asymmetry (FA) was dominated in asymmetric component. Although it is thought that primary cause of FA is developmental instability, our research has shown that phenotypic plasticity can also produce FA. Namely, replicas of flower organs with different orientations towards the sun had different values of asymmetric components of variance of the shape, as an outcome of plasticity in relation to the microenvironmental heterogeneity of ambient light. In this dissertation it is confirmed that size, shape and color of flowers are visual attractants for pollinators. Pairwase comparisons in size between pollinated and unpollinated flowers revealed that pollinated falls and standards are bigger related to their unpollinated pairs. Regression analyzes have shown that the size of the falls was under positive selection, while size of style branches was under negative direct selection. In contrast, the shape of the flower organs differed depending on their color, indicating that the pollinator's preference for specific combinations of visual signals was an important mechanism of morphological divergence of the reproductive organs of I. pumila.
PB  - Belgrade: University of Belgrade, Faculty of Biology
T2  - University of Belgrade, Faculty of Biology
T1  - Morfološko variranje, fenotipska plastičnost i fluktuirajuća asimetrija oblika cvetnih organa kod Iris pumila L.
T1  - Morphological variation, phenotypic plasticity and fluctuating asymmetry of floral organ shapes in Iris pumila L.
SP  - 1
EP  - 150
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3258
ER  - 

@phdthesis{
author = "Budečević, Sanja",
year = "2019",
abstract = "Analize unutar - i među - individualnihih razlika morfoloških struktura omogućuju otkrivanje obrazaca fenotipskog variranja, kao i ekološko - evolucionih mehanizama koji dovode do njihove divergencije. U ovoj disertaciji utvrđene su komponente fenotipske varijanse oblika funkcionalno različitih cvetnih organa kod entomofilne biljne vrste Iris pumila L., polimorfne u odnosu na boju cveta. Eksperimenti su vršeni na biljkama gajenim u eksperimentalnoj bašti, poreklom iz recipročnih ukrštanja 24 klonalna genotipa. Metodama geometrijske morfometrije utvrđeni su obrasci variranja oblika tri bilateralno simetrična organa: fola, standarda i grane tučka. Kod sva tri organa udeo simetrične komponente u ukupnom variranju oblika bio je najveći, dok je fluktuirajuća asimetrija (FA) dominirala u asimetričnoj komponenti. Iako se smatra da je primarni uzrok FA nestabilnost razvića, naša istraživanja su pokazala da fenotipska plastičnost može takođe da proizvede FA. Naime, replike cvetnih organa sa različitom orijentacijom prema suncu imale su različite vrednosti asimetričnih komponenti varijanse oblika, kao rezultat plastičnosti u odnosu na mikrosredinsku heterogenost ambijentalne svetlosti. Da veličina, oblik i boja cveta predstavljaju vizuelne signale za privlačenje potencijalnih oprašivača potvrdila su poređenja veličine cvetnih organa oprašenih i neoprašenih biljaka. Oprašeni cvetovi su, po pravilu, imali veće organe od neoprašenih, sa izuzetkom grane tučka. Regresione analize su pokazale da je veličina fola bila pod delovanjem pozitivne, a grane tučka negativne direkcione selekcije. Suprotno tome, oblik cvetnih organa se razlikovao u zavisnosti od boje, što ukazuje da je preferencija oprašivača prema specifičnim kombinacijama vizuelnih signala bila važan mehanizam morfološke divergencije reproduktivnih organa I. pumila., Analyzes of intra - and inter - individual differences of morphological structures allow the detection of patterns of phenotypic variation, as well as ecological evolutionary mechanisms leading to their divergence. In this dissertation, components of the phenotypic variation of the shape of functionally distinctive flower organs were determined in the entomophilous plant, specific for striking flower color polymorphism, Iris pumila L. Experiments were carried out on plants grown in experimental garden, originating from reciprocal crossing of 24 clonal genotypes. Using the methods of geometric morphometrics patterns of variation of the shape of three bilaterally symmetrical organs (falls, standards and style branches) have been revealed. In all three floral organs, the proportion of the symmetric component in the overall shape variation was the highest, while the fluctuating asymmetry (FA) was dominated in asymmetric component. Although it is thought that primary cause of FA is developmental instability, our research has shown that phenotypic plasticity can also produce FA. Namely, replicas of flower organs with different orientations towards the sun had different values of asymmetric components of variance of the shape, as an outcome of plasticity in relation to the microenvironmental heterogeneity of ambient light. In this dissertation it is confirmed that size, shape and color of flowers are visual attractants for pollinators. Pairwase comparisons in size between pollinated and unpollinated flowers revealed that pollinated falls and standards are bigger related to their unpollinated pairs. Regression analyzes have shown that the size of the falls was under positive selection, while size of style branches was under negative direct selection. In contrast, the shape of the flower organs differed depending on their color, indicating that the pollinator's preference for specific combinations of visual signals was an important mechanism of morphological divergence of the reproductive organs of I. pumila.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "University of Belgrade, Faculty of Biology",
title = "Morfološko variranje, fenotipska plastičnost i fluktuirajuća asimetrija oblika cvetnih organa kod Iris pumila L., Morphological variation, phenotypic plasticity and fluctuating asymmetry of floral organ shapes in Iris pumila L.",
pages = "1-150",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3258"
}

Budečević, S.. (2019). Morfološko variranje, fenotipska plastičnost i fluktuirajuća asimetrija oblika cvetnih organa kod Iris pumila L.. in University of Belgrade, Faculty of Biology
Belgrade: University of Belgrade, Faculty of Biology., 1-150.
https://hdl.handle.net/21.15107/rcub_ibiss_3258

Budečević S. Morfološko variranje, fenotipska plastičnost i fluktuirajuća asimetrija oblika cvetnih organa kod Iris pumila L.. in University of Belgrade, Faculty of Biology. 2019;:1-150.
https://hdl.handle.net/21.15107/rcub_ibiss_3258 .

Budečević, Sanja, "Morfološko variranje, fenotipska plastičnost i fluktuirajuća asimetrija oblika cvetnih organa kod Iris pumila L." in University of Belgrade, Faculty of Biology (2019):1-150,
https://hdl.handle.net/21.15107/rcub_ibiss_3258 .

TY  - CONF
AU  - Hočevar, Katarina
AU  - Budečević, Sanja
AU  - Vuleta, Ana
AU  - Manitašević Jovanović, Sanja
PY  - 2019
UR  - https://app.oxfordabstracts.com/events/653/program-app/submission/123447
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3546
AB  - To predict short-term plant responses to global warming, an open-topped-chamber experiment was conducted in two sun-exposed natural populations of Iris pumila during a growing season. In each population, one half of all randomly selected clonal plants were experimentally warmed using a clear-sided, open-topped chamber (OTC), while the other half experienced the ambient temperature conditions. The air temperature within the OTCs was approx.1-2 ºC higher than that outside of the OTCs in general. In spring and summer of 2018, phenotypic values of the three functional leaf traits: specific leaf area (SLA), leaf dry mass content (LDMC), and leaf water content (LWC) were recorded on the ramets from each clone growing inside and outside of the OTC. The phenotypic expressions of all analyzed traits changed significantly with temperature in both populations, but in an opposite direction. While SLA and LWC tended to be greater in the ramets developing within the OTCs relative to those growing outside of them, their LDMC values reversed, being higher inside than outside of the OTCs. The analyzed traits varied seasonally as well. Regardless of the location within a clone, the values of SLA and LWC were greater in spring then in summer. Conversely, LDMC reached its maximum in the warmer season. Our results clearly suggest that I. pumila plants have the capacity to cope with increasing temperatures by plastic responses of leaf functional traits. This finding is of crucial importance to forecast the fate of Iris populations under global warming - that is, whether they will adopt or disappear.
PB  - The European Society for Evolutionary Biology
C3  - 17th Congress of the European Society for Evolutionary Biology
T1  - Predicting ecological responses to global warming in Iris pumila: an open-topped chamber experiment
SP  - S36e.P12
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3546
ER  - 

@conference{
author = "Hočevar, Katarina and Budečević, Sanja and Vuleta, Ana and Manitašević Jovanović, Sanja",
year = "2019",
abstract = "To predict short-term plant responses to global warming, an open-topped-chamber experiment was conducted in two sun-exposed natural populations of Iris pumila during a growing season. In each population, one half of all randomly selected clonal plants were experimentally warmed using a clear-sided, open-topped chamber (OTC), while the other half experienced the ambient temperature conditions. The air temperature within the OTCs was approx.1-2 ºC higher than that outside of the OTCs in general. In spring and summer of 2018, phenotypic values of the three functional leaf traits: specific leaf area (SLA), leaf dry mass content (LDMC), and leaf water content (LWC) were recorded on the ramets from each clone growing inside and outside of the OTC. The phenotypic expressions of all analyzed traits changed significantly with temperature in both populations, but in an opposite direction. While SLA and LWC tended to be greater in the ramets developing within the OTCs relative to those growing outside of them, their LDMC values reversed, being higher inside than outside of the OTCs. The analyzed traits varied seasonally as well. Regardless of the location within a clone, the values of SLA and LWC were greater in spring then in summer. Conversely, LDMC reached its maximum in the warmer season. Our results clearly suggest that I. pumila plants have the capacity to cope with increasing temperatures by plastic responses of leaf functional traits. This finding is of crucial importance to forecast the fate of Iris populations under global warming - that is, whether they will adopt or disappear.",
publisher = "The European Society for Evolutionary Biology",
journal = "17th Congress of the European Society for Evolutionary Biology",
title = "Predicting ecological responses to global warming in Iris pumila: an open-topped chamber experiment",
pages = "S36e.P12",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3546"
}

Hočevar, K., Budečević, S., Vuleta, A.,& Manitašević Jovanović, S.. (2019). Predicting ecological responses to global warming in Iris pumila: an open-topped chamber experiment. in 17th Congress of the European Society for Evolutionary Biology
The European Society for Evolutionary Biology., S36e.P12.
https://hdl.handle.net/21.15107/rcub_ibiss_3546

Hočevar K, Budečević S, Vuleta A, Manitašević Jovanović S. Predicting ecological responses to global warming in Iris pumila: an open-topped chamber experiment. in 17th Congress of the European Society for Evolutionary Biology. 2019;:S36e.P12.
https://hdl.handle.net/21.15107/rcub_ibiss_3546 .

Hočevar, Katarina, Budečević, Sanja, Vuleta, Ana, Manitašević Jovanović, Sanja, "Predicting ecological responses to global warming in Iris pumila: an open-topped chamber experiment" in 17th Congress of the European Society for Evolutionary Biology (2019):S36e.P12,
https://hdl.handle.net/21.15107/rcub_ibiss_3546 .

TY  - CONF
AU  - Budečević, Sanja
AU  - Manitašević Jovanović, Sanja
AU  - Vuleta, Ana
AU  - Hočevar, Katarina
AU  - Tucić, Branka
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5061
AB  - Flowers are complex phenotypic structures composed of four functionally distinct organs arranged in concentric floral whorls: sepals in the frst, petals in the second, and two inner whorls
consisting of reproductive structures: stamens in the third whorl and tricarpellary gynoecium, in
the centre. Color, size and shape of these floral organs are considered to be important signals that
can attract the pollinators, advertising the plants’ floral reward (commonly nectar and pollen).
Here we use the methods of geometric morphometrics to compare size and shape variation of
three functionally different floral organs: falls, standards and style arms, among fve distinct color
morphs (blue, violet, dark blue, dark violet and yellow). Pairwise comparisons of the centroid size
means showed that all floral organs differed signifcantly among the analysed color phenotypes.
The mean shape of falls, standards and style arms appeared to be flower color-specifc as well, but
most noticeably between yellow - on one side, and blue/violet morphs - on the other. Procrustes
ANOVAs revealed the signifcant presence of directional and fluctuating asymmetry for falls and
standards among all fve color morphs, with the exception of style arms. The observed morphological diversifcation of floral organs among distinctive I. pumila color phenotypes could be the
adaptive outcome of the interactions with their pollinators.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Morphological diversity of functionally distinctive floral organs in Iris pumila: Does the flower color matter?
SP  - 82
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5061
ER  - 

@conference{
author = "Budečević, Sanja and Manitašević Jovanović, Sanja and Vuleta, Ana and Hočevar, Katarina and Tucić, Branka",
year = "2018",
abstract = "Flowers are complex phenotypic structures composed of four functionally distinct organs arranged in concentric floral whorls: sepals in the frst, petals in the second, and two inner whorls
consisting of reproductive structures: stamens in the third whorl and tricarpellary gynoecium, in
the centre. Color, size and shape of these floral organs are considered to be important signals that
can attract the pollinators, advertising the plants’ floral reward (commonly nectar and pollen).
Here we use the methods of geometric morphometrics to compare size and shape variation of
three functionally different floral organs: falls, standards and style arms, among fve distinct color
morphs (blue, violet, dark blue, dark violet and yellow). Pairwise comparisons of the centroid size
means showed that all floral organs differed signifcantly among the analysed color phenotypes.
The mean shape of falls, standards and style arms appeared to be flower color-specifc as well, but
most noticeably between yellow - on one side, and blue/violet morphs - on the other. Procrustes
ANOVAs revealed the signifcant presence of directional and fluctuating asymmetry for falls and
standards among all fve color morphs, with the exception of style arms. The observed morphological diversifcation of floral organs among distinctive I. pumila color phenotypes could be the
adaptive outcome of the interactions with their pollinators.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Morphological diversity of functionally distinctive floral organs in Iris pumila: Does the flower color matter?",
pages = "82",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5061"
}

Budečević, S., Manitašević Jovanović, S., Vuleta, A., Hočevar, K.,& Tucić, B.. (2018). Morphological diversity of functionally distinctive floral organs in Iris pumila: Does the flower color matter?. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 82.
https://hdl.handle.net/21.15107/rcub_ibiss_5061

Budečević S, Manitašević Jovanović S, Vuleta A, Hočevar K, Tucić B. Morphological diversity of functionally distinctive floral organs in Iris pumila: Does the flower color matter?. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:82.
https://hdl.handle.net/21.15107/rcub_ibiss_5061 .

Budečević, Sanja, Manitašević Jovanović, Sanja, Vuleta, Ana, Hočevar, Katarina, Tucić, Branka, "Morphological diversity of functionally distinctive floral organs in Iris pumila: Does the flower color matter?" in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):82,
https://hdl.handle.net/21.15107/rcub_ibiss_5061 .

TY  - CONF
AU  - Vuleta, Ana
AU  - Budečević, Sanja
AU  - Manitašević Jovanović, Sanja
AU  - Tucić, Branka
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5062
AB  - Angiosperm flowers serve as an advertisement for pollinators and are, therefore, believed to
be shaped by pollinator-mediated selection. The large flower size, which is one of the distinct
characteristics of the genus Iris, might have evolved under the strong selective pressures imposed
by pollinators, either because larger flowers indicate more rewards or because the pollinators can
detect them from a greater distance. To test the role of visual floral signals in attracting pollinators and, consequently, pollination efciency, we compared the phenotypic expressions of flower
height and centroid size of petaloid floral organs: falls, standards and style arms, as well as anthocyanin absorption between naturally pollinated and non-pollinated flowers of I. pumila plants
grown in a common-garden experiment. Our results indicated that I. pumila pollinators generally
preferred taller flowers with greater organ sizes compared to the alternative ones. However, the
direction of pollinator-mediated selection appeared to be strongly flower organ-specifc: positive
on fall, negative on style arm and neutral on standard size. The observed results are in agreement
with the functions that each of these floral structures has in the pollination process: standards are
a long-distance reward signals, falls are landing platform for pollinating insects, while style arms,
as upper parts of the pollination tunnels, promote pollen deposition. We failed to corroborate the
existence of pollinator-mediated selection on anthocyanin absorption. This suggested that some
other aspects of flower colour might be more attractive to insect pollinators, or that different biotic or abiotic factors could account for the maintenance of flower colour polymorphism in I. pumila.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Do pollinators prefer bigger floral organs? A case study on Iris pumila L.
SP  - 86
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5062
ER  - 

@conference{
author = "Vuleta, Ana and Budečević, Sanja and Manitašević Jovanović, Sanja and Tucić, Branka",
year = "2018",
abstract = "Angiosperm flowers serve as an advertisement for pollinators and are, therefore, believed to
be shaped by pollinator-mediated selection. The large flower size, which is one of the distinct
characteristics of the genus Iris, might have evolved under the strong selective pressures imposed
by pollinators, either because larger flowers indicate more rewards or because the pollinators can
detect them from a greater distance. To test the role of visual floral signals in attracting pollinators and, consequently, pollination efciency, we compared the phenotypic expressions of flower
height and centroid size of petaloid floral organs: falls, standards and style arms, as well as anthocyanin absorption between naturally pollinated and non-pollinated flowers of I. pumila plants
grown in a common-garden experiment. Our results indicated that I. pumila pollinators generally
preferred taller flowers with greater organ sizes compared to the alternative ones. However, the
direction of pollinator-mediated selection appeared to be strongly flower organ-specifc: positive
on fall, negative on style arm and neutral on standard size. The observed results are in agreement
with the functions that each of these floral structures has in the pollination process: standards are
a long-distance reward signals, falls are landing platform for pollinating insects, while style arms,
as upper parts of the pollination tunnels, promote pollen deposition. We failed to corroborate the
existence of pollinator-mediated selection on anthocyanin absorption. This suggested that some
other aspects of flower colour might be more attractive to insect pollinators, or that different biotic or abiotic factors could account for the maintenance of flower colour polymorphism in I. pumila.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Do pollinators prefer bigger floral organs? A case study on Iris pumila L.",
pages = "86",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5062"
}

Vuleta, A., Budečević, S., Manitašević Jovanović, S.,& Tucić, B.. (2018). Do pollinators prefer bigger floral organs? A case study on Iris pumila L.. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 86.
https://hdl.handle.net/21.15107/rcub_ibiss_5062

Vuleta A, Budečević S, Manitašević Jovanović S, Tucić B. Do pollinators prefer bigger floral organs? A case study on Iris pumila L.. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:86.
https://hdl.handle.net/21.15107/rcub_ibiss_5062 .

Vuleta, Ana, Budečević, Sanja, Manitašević Jovanović, Sanja, Tucić, Branka, "Do pollinators prefer bigger floral organs? A case study on Iris pumila L." in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):86,
https://hdl.handle.net/21.15107/rcub_ibiss_5062 .

TY  - JOUR
AU  - Tucić, Branka
AU  - Budečević, Sanja
AU  - Manitašević Jovanović, Sanja
AU  - Vuleta, Ana
AU  - Klingenberg, Christian Peter
PY  - 2018
UR  - http://doi.wiley.com/10.1111/jeb.13207
UR  - http://www.ncbi.nlm.nih.gov/pubmed/29134739
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2927
AB  - Fluctuating asymmetry (FA) is widely used to quantify developmental instability (DI) in ecological and evolutionary studies. It has long been recognized that FA may not exclusively originate from DI for sessile organisms such as plants, because phenotypic plasticity in response to heterogeneities in the environment might also produce FA. This study provides the first empirical evidence for this hypothesis. We reasoned that solar irradiance, which is greater on the southern side than on the northern side of plants growing in the temperate zone of the Northern Hemisphere, would cause systematic morphological differences and asymmetry associated with the orientation of plant parts. We used geometric morphometrics to characterize the size and shape of flower parts in Iris pumila grown in a common garden. The size of floral organs was not significantly affected by orientation. Shape and particularly its asymmetric component differed significantly according to orientation for three different floral parts. Orientation accounted for 10.4% of the total shape asymmetry within flowers in the falls, for 11.4% in the standards and for 2.2% in the style branches. This indicates that phenotypic plasticity in response to a directed environmental factor, most likely solar irradiance, contributes to FA of flowers under natural conditions. That FA partly results from phenotypic plasticity and not just from DI needs to be considered by studies of FA in plants and other sessile organisms.
T2  - Journal of Evolutionary Biology
T1  - Phenotypic plasticity in response to environmental heterogeneity contributes to fluctuating asymmetry in plants: first empirical evidence.
IS  - 2
VL  - 31
DO  - 10.1111/jeb.13207
SP  - 197
EP  - 210
ER  - 

@article{
author = "Tucić, Branka and Budečević, Sanja and Manitašević Jovanović, Sanja and Vuleta, Ana and Klingenberg, Christian Peter",
year = "2018",
abstract = "Fluctuating asymmetry (FA) is widely used to quantify developmental instability (DI) in ecological and evolutionary studies. It has long been recognized that FA may not exclusively originate from DI for sessile organisms such as plants, because phenotypic plasticity in response to heterogeneities in the environment might also produce FA. This study provides the first empirical evidence for this hypothesis. We reasoned that solar irradiance, which is greater on the southern side than on the northern side of plants growing in the temperate zone of the Northern Hemisphere, would cause systematic morphological differences and asymmetry associated with the orientation of plant parts. We used geometric morphometrics to characterize the size and shape of flower parts in Iris pumila grown in a common garden. The size of floral organs was not significantly affected by orientation. Shape and particularly its asymmetric component differed significantly according to orientation for three different floral parts. Orientation accounted for 10.4% of the total shape asymmetry within flowers in the falls, for 11.4% in the standards and for 2.2% in the style branches. This indicates that phenotypic plasticity in response to a directed environmental factor, most likely solar irradiance, contributes to FA of flowers under natural conditions. That FA partly results from phenotypic plasticity and not just from DI needs to be considered by studies of FA in plants and other sessile organisms.",
journal = "Journal of Evolutionary Biology",
title = "Phenotypic plasticity in response to environmental heterogeneity contributes to fluctuating asymmetry in plants: first empirical evidence.",
number = "2",
volume = "31",
doi = "10.1111/jeb.13207",
pages = "197-210"
}

Tucić, B., Budečević, S., Manitašević Jovanović, S., Vuleta, A.,& Klingenberg, C. P.. (2018). Phenotypic plasticity in response to environmental heterogeneity contributes to fluctuating asymmetry in plants: first empirical evidence.. in Journal of Evolutionary Biology, 31(2), 197-210.
https://doi.org/10.1111/jeb.13207

Tucić B, Budečević S, Manitašević Jovanović S, Vuleta A, Klingenberg CP. Phenotypic plasticity in response to environmental heterogeneity contributes to fluctuating asymmetry in plants: first empirical evidence.. in Journal of Evolutionary Biology. 2018;31(2):197-210.
doi:10.1111/jeb.13207 .

Tucić, Branka, Budečević, Sanja, Manitašević Jovanović, Sanja, Vuleta, Ana, Klingenberg, Christian Peter, "Phenotypic plasticity in response to environmental heterogeneity contributes to fluctuating asymmetry in plants: first empirical evidence." in Journal of Evolutionary Biology, 31, no. 2 (2018):197-210,
https://doi.org/10.1111/jeb.13207 . .

TY  - CONF
AU  - Hočevar, Katarina
AU  - Budečević, Sanja
AU  - Manitašević Jovanović, Sanja
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5059
AB  - The 70kD heat shock proteins (Hsp70s) are highly conserved molecular chaperones that play
essential roles in numerous cellular processes, including the folding of newly synthesized and
stress-denatured proteins, the translocation of proteins across membranes, as well as the degradation of aggregated proteins. Here we examined the variation in the abundance of Hsp70s in
different floral organs of the three distinctive color morphs in Iris pumila. Flowers of twenty seven
genotypes raised in a common-garden and belonging to three colour classes: yellow, dark blue
and dark violet, were collected (9 per each class) for an immunoblot analysis. The content of Hsp70
was quantifed in four floral organs: falls, standards, stamens and style arms. The immunoblot analysis revealed that the expression of Hsp70s was floral organ-specifc. While only one Hsp70 isoform
was expressed in the standards and falls, the stamens and style arms expressed two isoforms. The
relative abundance of Hsp70 varied among differed flower organs as well. The lowest value was
recorded in the standards and the highest in the stamens, whereas its abundance in the falls and
style arms appeared to be intermediate. Such variation pattern was detected exclusively in the
dark violet colour morphs. In the yellow flowers, however, the amount of Hsp70 tended to be lower compared to the dark blue or dark violet colour morphs. This study provides original evidence
for organ-specifc Hsp70 expression in flowers of I. pumila plants, likely due to the differential metabolic activities occurring in the cells of these functionally distinctive floral structures.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Variation in the amount of Hsp70 among different floral organs in three alternative color phenotypes of Iris pumila
SP  - 89
EP  - 90
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5059
ER  - 

@conference{
author = "Hočevar, Katarina and Budečević, Sanja and Manitašević Jovanović, Sanja",
year = "2018",
abstract = "The 70kD heat shock proteins (Hsp70s) are highly conserved molecular chaperones that play
essential roles in numerous cellular processes, including the folding of newly synthesized and
stress-denatured proteins, the translocation of proteins across membranes, as well as the degradation of aggregated proteins. Here we examined the variation in the abundance of Hsp70s in
different floral organs of the three distinctive color morphs in Iris pumila. Flowers of twenty seven
genotypes raised in a common-garden and belonging to three colour classes: yellow, dark blue
and dark violet, were collected (9 per each class) for an immunoblot analysis. The content of Hsp70
was quantifed in four floral organs: falls, standards, stamens and style arms. The immunoblot analysis revealed that the expression of Hsp70s was floral organ-specifc. While only one Hsp70 isoform
was expressed in the standards and falls, the stamens and style arms expressed two isoforms. The
relative abundance of Hsp70 varied among differed flower organs as well. The lowest value was
recorded in the standards and the highest in the stamens, whereas its abundance in the falls and
style arms appeared to be intermediate. Such variation pattern was detected exclusively in the
dark violet colour morphs. In the yellow flowers, however, the amount of Hsp70 tended to be lower compared to the dark blue or dark violet colour morphs. This study provides original evidence
for organ-specifc Hsp70 expression in flowers of I. pumila plants, likely due to the differential metabolic activities occurring in the cells of these functionally distinctive floral structures.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Variation in the amount of Hsp70 among different floral organs in three alternative color phenotypes of Iris pumila",
pages = "89-90",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5059"
}

Hočevar, K., Budečević, S.,& Manitašević Jovanović, S.. (2018). Variation in the amount of Hsp70 among different floral organs in three alternative color phenotypes of Iris pumila. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 89-90.
https://hdl.handle.net/21.15107/rcub_ibiss_5059

Hočevar K, Budečević S, Manitašević Jovanović S. Variation in the amount of Hsp70 among different floral organs in three alternative color phenotypes of Iris pumila. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:89-90.
https://hdl.handle.net/21.15107/rcub_ibiss_5059 .

Hočevar, Katarina, Budečević, Sanja, Manitašević Jovanović, Sanja, "Variation in the amount of Hsp70 among different floral organs in three alternative color phenotypes of Iris pumila" in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):89-90,
https://hdl.handle.net/21.15107/rcub_ibiss_5059 .

TY  - CONF
AU  - Hočevar, Katarina
AU  - Budečević, Sanja
AU  - Manitašević Jovanović, Sanja
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5060
AB  - The 70kD heat shock proteins (Hsp70s) are highly conserved molecular chaperones that playessential roles in numerous cellular processes, including the folding of newly synthesized andstress-denatured proteins, the translocation of proteins across membranes, as well as the degradation of aggregated proteins. Here we examined the variation in the abundance of Hsp70s indifferent floral organs of the three distinctive color morphs in Iris pumila. Flowers of twenty sevengenotypes raised in a common-garden and belonging to three colour classes: yellow, dark blueand dark violet, were collected (9 per each class) for an immunoblot analysis. The content of Hsp70was quantifed in four floral organs: falls, standards, stamens and style arms. The immunoblot analysis revealed that the expression of Hsp70s was floral organ-specifc. While only one Hsp70 isoformwas expressed in the standards and falls, the stamens and style arms expressed two isoforms. Therelative abundance of Hsp70 varied among differed flower organs as well. The lowest value wasrecorded in the standards and the highest in the stamens, whereas its abundance in the falls andstyle arms appeared to be intermediate. Such variation pattern was detected exclusively in thedark violet colour morphs. In the yellow flowers, however, the amount of Hsp70 tended to be lower compared to the dark blue or dark violet colour morphs. This study provides original evidencefor organ-specifc Hsp70 expression in flowers of I. pumila plants, likely due to the differential metabolic activities occurring in the cells of these functionally distinctive floral structures.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković" - National Institute of the Republic of Serbia, University of Belgrade
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Variation in the amount of Hsp70 among different floral organs in three alternative color phenotypes of Iris pumila
SP  - PP3-8
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5060
ER  - 

@conference{
author = "Hočevar, Katarina and Budečević, Sanja and Manitašević Jovanović, Sanja",
year = "2018",
abstract = "The 70kD heat shock proteins (Hsp70s) are highly conserved molecular chaperones that playessential roles in numerous cellular processes, including the folding of newly synthesized andstress-denatured proteins, the translocation of proteins across membranes, as well as the degradation of aggregated proteins. Here we examined the variation in the abundance of Hsp70s indifferent floral organs of the three distinctive color morphs in Iris pumila. Flowers of twenty sevengenotypes raised in a common-garden and belonging to three colour classes: yellow, dark blueand dark violet, were collected (9 per each class) for an immunoblot analysis. The content of Hsp70was quantifed in four floral organs: falls, standards, stamens and style arms. The immunoblot analysis revealed that the expression of Hsp70s was floral organ-specifc. While only one Hsp70 isoformwas expressed in the standards and falls, the stamens and style arms expressed two isoforms. Therelative abundance of Hsp70 varied among differed flower organs as well. The lowest value wasrecorded in the standards and the highest in the stamens, whereas its abundance in the falls andstyle arms appeared to be intermediate. Such variation pattern was detected exclusively in thedark violet colour morphs. In the yellow flowers, however, the amount of Hsp70 tended to be lower compared to the dark blue or dark violet colour morphs. This study provides original evidencefor organ-specifc Hsp70 expression in flowers of I. pumila plants, likely due to the differential metabolic activities occurring in the cells of these functionally distinctive floral structures.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković" - National Institute of the Republic of Serbia, University of Belgrade",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Variation in the amount of Hsp70 among different floral organs in three alternative color phenotypes of Iris pumila",
pages = "PP3-8",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5060"
}

Hočevar, K., Budečević, S.,& Manitašević Jovanović, S.. (2018). Variation in the amount of Hsp70 among different floral organs in three alternative color phenotypes of Iris pumila. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković" - National Institute of the Republic of Serbia, University of Belgrade., PP3-8.
https://hdl.handle.net/21.15107/rcub_ibiss_5060

Hočevar K, Budečević S, Manitašević Jovanović S. Variation in the amount of Hsp70 among different floral organs in three alternative color phenotypes of Iris pumila. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:PP3-8.
https://hdl.handle.net/21.15107/rcub_ibiss_5060 .

Hočevar, Katarina, Budečević, Sanja, Manitašević Jovanović, Sanja, "Variation in the amount of Hsp70 among different floral organs in three alternative color phenotypes of Iris pumila" in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):PP3-8,
https://hdl.handle.net/21.15107/rcub_ibiss_5060 .

TY  - CONF
AU  - Manitašević Jovanović, Sanja
AU  - Hočevar, Katarina
AU  - Budečević, Sanja
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5057
AB  - Flavonoids and carotenoids are plant pigments that play a variety of functional roles in higher
plants. Beside their impacts on flower color, they are involved in plant growth, development and
protection against a wide range of environmental stressors. They act either as light attenuators
and free radical scavengers (e.g. anthocyanins) or participate in non-photochemical quenching
(carotenoids). To quantify the accumulation of flavonoids and carotenoids in different floral organs,
ffty Iris pumila genotypes grown in a common-garden and belonging to different color classes
(yellow, blue, violet, dark blue and dark violet) were assessed. One flower per each genotype was
harvested and analyzed spectrophotometrically for the content of three flavonoids (anthocyanins,
flavones, flavonols) and total carotenoids in different floral organs: falls, standards, stamens and
style arms. While the quantity of flavonoids was found to be floral organ- and color morph-specific, the amount of carotenoids varied only among individual floral organs. The variation pattern of
the analyzed compounds displayed identical trend - the lowest amounts were recorded in the stamen and style arm, and the highest in the standard and fall. The content of carotenoids appeared
to be the highest in floral organs of the yellow color morphs, whereas in the blue, violet, dark blue
and dark violet flower phenotypes its abundance was a half of that recorded for the yellow one.
Conversely, the anthocyanin content was rather high in the floral organs of dark violet and dark
blue color morphs, but extremely low in those of the yellow one. Our results clearly suggest that a
greater accumulation of both plant pigments in sterile rather than in fertile flower organs could be
causally related to their specifc function in the process of sexual reproduction, where the former
serve to attract plant pollinators, while the latter promote an efcient pollen deposition.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Flavonoid and carotenoid content in floral organs of Iris pumila
SP  - 93
EP  - 94
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5057
ER  - 

@conference{
author = "Manitašević Jovanović, Sanja and Hočevar, Katarina and Budečević, Sanja",
year = "2018",
abstract = "Flavonoids and carotenoids are plant pigments that play a variety of functional roles in higher
plants. Beside their impacts on flower color, they are involved in plant growth, development and
protection against a wide range of environmental stressors. They act either as light attenuators
and free radical scavengers (e.g. anthocyanins) or participate in non-photochemical quenching
(carotenoids). To quantify the accumulation of flavonoids and carotenoids in different floral organs,
ffty Iris pumila genotypes grown in a common-garden and belonging to different color classes
(yellow, blue, violet, dark blue and dark violet) were assessed. One flower per each genotype was
harvested and analyzed spectrophotometrically for the content of three flavonoids (anthocyanins,
flavones, flavonols) and total carotenoids in different floral organs: falls, standards, stamens and
style arms. While the quantity of flavonoids was found to be floral organ- and color morph-specific, the amount of carotenoids varied only among individual floral organs. The variation pattern of
the analyzed compounds displayed identical trend - the lowest amounts were recorded in the stamen and style arm, and the highest in the standard and fall. The content of carotenoids appeared
to be the highest in floral organs of the yellow color morphs, whereas in the blue, violet, dark blue
and dark violet flower phenotypes its abundance was a half of that recorded for the yellow one.
Conversely, the anthocyanin content was rather high in the floral organs of dark violet and dark
blue color morphs, but extremely low in those of the yellow one. Our results clearly suggest that a
greater accumulation of both plant pigments in sterile rather than in fertile flower organs could be
causally related to their specifc function in the process of sexual reproduction, where the former
serve to attract plant pollinators, while the latter promote an efcient pollen deposition.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Flavonoid and carotenoid content in floral organs of Iris pumila",
pages = "93-94",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5057"
}

Manitašević Jovanović, S., Hočevar, K.,& Budečević, S.. (2018). Flavonoid and carotenoid content in floral organs of Iris pumila. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 93-94.
https://hdl.handle.net/21.15107/rcub_ibiss_5057

Manitašević Jovanović S, Hočevar K, Budečević S. Flavonoid and carotenoid content in floral organs of Iris pumila. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:93-94.
https://hdl.handle.net/21.15107/rcub_ibiss_5057 .

Manitašević Jovanović, Sanja, Hočevar, Katarina, Budečević, Sanja, "Flavonoid and carotenoid content in floral organs of Iris pumila" in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):93-94,
https://hdl.handle.net/21.15107/rcub_ibiss_5057 .

TY  - CONF
AU  - Manitašević Jovanović, Sanja
AU  - Hočevar, Katarina
AU  - Budečević, Sanja
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5058
AB  - Flavonoids and carotenoids are plant pigments that play a variety of functional roles in higherplants. Beside their impacts on flower color, they are involved in plant growth, development andprotection against a wide range of environmental stressors. They act either as light attenuatorsand free radical scavengers (e.g. anthocyanins) or participate in non-photochemical quenching(carotenoids). To quantify the accumulation of flavonoids and carotenoids in different floral organs,ffty Iris pumila genotypes grown in a common-garden and belonging to different color classes(yellow, blue, violet, dark blue and dark violet) were assessed. One flower per each genotype washarvested and analyzed spectrophotometrically for the content of three flavonoids (anthocyanins,flavones, flavonols) and total carotenoids in different floral organs: falls, standards, stamens andstyle arms. While the quantity of flavonoids was found to be floral organ- and color morph-specific, the amount of carotenoids varied only among individual floral organs. The variation pattern ofthe analyzed compounds displayed identical trend - the lowest amounts were recorded in the stamen and style arm, and the highest in the standard and fall. The content of carotenoids appearedto be the highest in floral organs of the yellow color morphs, whereas in the blue, violet, dark blueand dark violet flower phenotypes its abundance was a half of that recorded for the yellow one.Conversely, the anthocyanin content was rather high in the floral organs of dark violet and darkblue color morphs, but extremely low in those of the yellow one. Our results clearly suggest that agreater accumulation of both plant pigments in sterile rather than in fertile flower organs could becausally related to their specifc function in the process of sexual reproduction, where the formerserve to attract plant pollinators, while the latter promote an efcient pollen deposition.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković" - National Institute of the Republic of Serbia, University of Belgrade
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Flavonoid and carotenoid content in floral organs of Iris pumila
SP  - PP3-14
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5058
ER  - 

@conference{
author = "Manitašević Jovanović, Sanja and Hočevar, Katarina and Budečević, Sanja",
year = "2018",
abstract = "Flavonoids and carotenoids are plant pigments that play a variety of functional roles in higherplants. Beside their impacts on flower color, they are involved in plant growth, development andprotection against a wide range of environmental stressors. They act either as light attenuatorsand free radical scavengers (e.g. anthocyanins) or participate in non-photochemical quenching(carotenoids). To quantify the accumulation of flavonoids and carotenoids in different floral organs,ffty Iris pumila genotypes grown in a common-garden and belonging to different color classes(yellow, blue, violet, dark blue and dark violet) were assessed. One flower per each genotype washarvested and analyzed spectrophotometrically for the content of three flavonoids (anthocyanins,flavones, flavonols) and total carotenoids in different floral organs: falls, standards, stamens andstyle arms. While the quantity of flavonoids was found to be floral organ- and color morph-specific, the amount of carotenoids varied only among individual floral organs. The variation pattern ofthe analyzed compounds displayed identical trend - the lowest amounts were recorded in the stamen and style arm, and the highest in the standard and fall. The content of carotenoids appearedto be the highest in floral organs of the yellow color morphs, whereas in the blue, violet, dark blueand dark violet flower phenotypes its abundance was a half of that recorded for the yellow one.Conversely, the anthocyanin content was rather high in the floral organs of dark violet and darkblue color morphs, but extremely low in those of the yellow one. Our results clearly suggest that agreater accumulation of both plant pigments in sterile rather than in fertile flower organs could becausally related to their specifc function in the process of sexual reproduction, where the formerserve to attract plant pollinators, while the latter promote an efcient pollen deposition.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković" - National Institute of the Republic of Serbia, University of Belgrade",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Flavonoid and carotenoid content in floral organs of Iris pumila",
pages = "PP3-14",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5058"
}

Manitašević Jovanović, S., Hočevar, K.,& Budečević, S.. (2018). Flavonoid and carotenoid content in floral organs of Iris pumila. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković" - National Institute of the Republic of Serbia, University of Belgrade., PP3-14.
https://hdl.handle.net/21.15107/rcub_ibiss_5058

Manitašević Jovanović S, Hočevar K, Budečević S. Flavonoid and carotenoid content in floral organs of Iris pumila. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:PP3-14.
https://hdl.handle.net/21.15107/rcub_ibiss_5058 .

Manitašević Jovanović, Sanja, Hočevar, Katarina, Budečević, Sanja, "Flavonoid and carotenoid content in floral organs of Iris pumila" in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):PP3-14,
https://hdl.handle.net/21.15107/rcub_ibiss_5058 .

TY  - JOUR
AU  - Souto-Vilarósa, Daniel
AU  - Vuleta, Ana
AU  - Manitašević Jovanović, Sanja
AU  - Budečević, Sanja
AU  - Wang, Hui
AU  - Sapir, Yuval
AU  - Imbert, Eric
PY  - 2018
UR  - http://doi.wiley.com/10.1111/oik.04501
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2958
AB  - Community assembly rules have been extensively studied, but its association with regional environmental variation, while land use history remains largely unexplored. Land use history might be especially important in Mediterranean forests, considering their historical deforestation and recent afforestation. Using forest inventories and historical (1956) and recent (2000) land cover maps, we explored the following hypotheses: 1) woody species assembly is driven by environmental factors, but also by historical landscape attributes; 2) recent forests exhibit lower woody species richness than pre-existing due to the existence of colonization credits; 3) these credits are modulated by species’ life-forms and dispersal mechanisms. We examined the association of forest historical type (pre-existing versus recent) with total species richness and that of diverse life-forms and dispersal groups, also considering the effects of current environment and past landscape factors. When accounting for these effects, no significant differences in woody species richness were found between forest historical types except for vertebrate-dispersed species. Species richness of this group was affected by the interaction of forest historical type with distance to coast and rainfall: vertebrate-dispersed species richness increased with rainfall and distance to the coast in recent forests, while it was higher in dryer sites in pre-existing forests. In addition, forest historical types showed differences in woody species composition associated to diverse environmental and past landscape factors. In view of these results we can conclude that: 1) community assembly in terms of species richness is fast enough to exhaust most colonization credit in recent Mediterranean forests except for vertebrate-dispersed species; 2) for these species, colonization credit is affected by the interplay of forest history and a set of proxies of niche and landscape constraints of species dispersal and establishment; 3) woody species assemblage is mostly shaped by the species’ ecological niches in these forests.
T2  - Oikos
T2  - Oikos
T1  - Are pollinators the agents of selection on flower colour and size in irises?
IS  - 6
VL  - 127
DO  - 10.1111/oik.04501
SP  - 834
EP  - 846
ER  - 

@article{
author = "Souto-Vilarósa, Daniel and Vuleta, Ana and Manitašević Jovanović, Sanja and Budečević, Sanja and Wang, Hui and Sapir, Yuval and Imbert, Eric",
year = "2018",
abstract = "Community assembly rules have been extensively studied, but its association with regional environmental variation, while land use history remains largely unexplored. Land use history might be especially important in Mediterranean forests, considering their historical deforestation and recent afforestation. Using forest inventories and historical (1956) and recent (2000) land cover maps, we explored the following hypotheses: 1) woody species assembly is driven by environmental factors, but also by historical landscape attributes; 2) recent forests exhibit lower woody species richness than pre-existing due to the existence of colonization credits; 3) these credits are modulated by species’ life-forms and dispersal mechanisms. We examined the association of forest historical type (pre-existing versus recent) with total species richness and that of diverse life-forms and dispersal groups, also considering the effects of current environment and past landscape factors. When accounting for these effects, no significant differences in woody species richness were found between forest historical types except for vertebrate-dispersed species. Species richness of this group was affected by the interaction of forest historical type with distance to coast and rainfall: vertebrate-dispersed species richness increased with rainfall and distance to the coast in recent forests, while it was higher in dryer sites in pre-existing forests. In addition, forest historical types showed differences in woody species composition associated to diverse environmental and past landscape factors. In view of these results we can conclude that: 1) community assembly in terms of species richness is fast enough to exhaust most colonization credit in recent Mediterranean forests except for vertebrate-dispersed species; 2) for these species, colonization credit is affected by the interplay of forest history and a set of proxies of niche and landscape constraints of species dispersal and establishment; 3) woody species assemblage is mostly shaped by the species’ ecological niches in these forests.",
journal = "Oikos, Oikos",
title = "Are pollinators the agents of selection on flower colour and size in irises?",
number = "6",
volume = "127",
doi = "10.1111/oik.04501",
pages = "834-846"
}

Souto-Vilarósa, D., Vuleta, A., Manitašević Jovanović, S., Budečević, S., Wang, H., Sapir, Y.,& Imbert, E.. (2018). Are pollinators the agents of selection on flower colour and size in irises?. in Oikos, 127(6), 834-846.
https://doi.org/10.1111/oik.04501

Souto-Vilarósa D, Vuleta A, Manitašević Jovanović S, Budečević S, Wang H, Sapir Y, Imbert E. Are pollinators the agents of selection on flower colour and size in irises?. in Oikos. 2018;127(6):834-846.
doi:10.1111/oik.04501 .

Souto-Vilarósa, Daniel, Vuleta, Ana, Manitašević Jovanović, Sanja, Budečević, Sanja, Wang, Hui, Sapir, Yuval, Imbert, Eric, "Are pollinators the agents of selection on flower colour and size in irises?" in Oikos, 127, no. 6 (2018):834-846,
https://doi.org/10.1111/oik.04501 . .

TY  - JOUR
AU  - Martinossi-Allibert, Ivain
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Arnqvist, Göran
AU  - Stojković, Biljana
AU  - Berger, David
PY  - 2018
UR  - http://doi.wiley.com/10.1111/evo.13412
UR  - http://www.ncbi.nlm.nih.gov/pubmed/29238970
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2963
AB  - Whether sexual selection generally promotes or impedes population persistence remains an open question. Intralocus sexual conflict (IaSC) can render sexual selection in males detrimental to the population by increasing the frequency of alleles with positive effects on male reproductive success but negative effects on female fecundity. Recent modeling based on fitness landscape theory, however, indicates that the relative impact of IaSC may be reduced in maladapted populations and that sexual selection therefore might promote adaptation when it is most needed. Here, we test this prediction using bean beetles that had undergone 80 generations of experimental evolution on two alternative host plants. We isolated and assessed the effect of maladaptation on sex-specific strengths of selection and IaSC by cross-rearing the two experimental evolution regimes on the alternative hosts and estimating within-population genetic (co)variance for fitness in males and females. Two key predictions were upheld: males generally experienced stronger selection compared to females and maladaptation increased selection in females. However, maladaptation consistently decreased male-bias in the strength of selection and IaSC was not reduced in maladapted populations. These findings imply that sexual selection can be disrupted in stressful environmental conditions, thus reducing one of the potential benefits of sexual reproduction in maladapted populations.
T2  - Evolution
T1  - The consequences of sexual selection in well-adapted and maladapted populations of bean beetles.
DO  - 10.1111/evo.13412
ER  - 

@article{
author = "Martinossi-Allibert, Ivain and Savković, Uroš and Đorđević, Mirko and Arnqvist, Göran and Stojković, Biljana and Berger, David",
year = "2018",
abstract = "Whether sexual selection generally promotes or impedes population persistence remains an open question. Intralocus sexual conflict (IaSC) can render sexual selection in males detrimental to the population by increasing the frequency of alleles with positive effects on male reproductive success but negative effects on female fecundity. Recent modeling based on fitness landscape theory, however, indicates that the relative impact of IaSC may be reduced in maladapted populations and that sexual selection therefore might promote adaptation when it is most needed. Here, we test this prediction using bean beetles that had undergone 80 generations of experimental evolution on two alternative host plants. We isolated and assessed the effect of maladaptation on sex-specific strengths of selection and IaSC by cross-rearing the two experimental evolution regimes on the alternative hosts and estimating within-population genetic (co)variance for fitness in males and females. Two key predictions were upheld: males generally experienced stronger selection compared to females and maladaptation increased selection in females. However, maladaptation consistently decreased male-bias in the strength of selection and IaSC was not reduced in maladapted populations. These findings imply that sexual selection can be disrupted in stressful environmental conditions, thus reducing one of the potential benefits of sexual reproduction in maladapted populations.",
journal = "Evolution",
title = "The consequences of sexual selection in well-adapted and maladapted populations of bean beetles.",
doi = "10.1111/evo.13412"
}

Martinossi-Allibert, I., Savković, U., Đorđević, M., Arnqvist, G., Stojković, B.,& Berger, D.. (2018). The consequences of sexual selection in well-adapted and maladapted populations of bean beetles.. in Evolution.
https://doi.org/10.1111/evo.13412

Martinossi-Allibert I, Savković U, Đorđević M, Arnqvist G, Stojković B, Berger D. The consequences of sexual selection in well-adapted and maladapted populations of bean beetles.. in Evolution. 2018;.
doi:10.1111/evo.13412 .

Martinossi-Allibert, Ivain, Savković, Uroš, Đorđević, Mirko, Arnqvist, Göran, Stojković, Biljana, Berger, David, "The consequences of sexual selection in well-adapted and maladapted populations of bean beetles." in Evolution (2018),
https://doi.org/10.1111/evo.13412 . .

TY  - CONF
AU  - Hočevar, Katarina
AU  - Nolić, Vladimir
AU  - Budečević, Sanja
AU  - Manitašević Jovanović, Sanja
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6204
AB  - Introduction: Natural populations of the dwarf bearded iris, Iris pumila, are markedly
polymorphic for flower color. Selection pressures exerted by a range of abiotic and
biotic factors could be involved in maintaining the polymorphism. Here we
quantified the amount of the Hsp70 and the two groups of pigments with
antioxidative properties: anthocyanins and carotenoids.These molecules impact
abiotic stress tolerance, ultimately influencing the fitness of individual plants.
Methods: A total of fifty genotypes raised in a common-garden and belonging to a
corresponding color class (yellow, blue, violet, dark blue and dark violet) were
surveyed. One fully developed leaf and a flower from each genotype were
analyzed for the Hsp70 amount and pigments concentration.
Results: The Western blot analysis revealed the presence of one isoform for the Hsp70
in leaf and two isoforms in flower organs. In both vegetative and reproductive tissues
the amount of Hsp70 was found to be the lowest in yellow colored genotypes
compared to other color classes. In violet and blue flowers, the concentration of
Hsp70 decreased gradually from light to dark colored variants. Conversely, the
concentration of anthocyanins was found to be higher in darker than in the lighter
color morphs. An inverse trend was observed for the total carotenoids
concentration.
Conclusion: This study revealed that each I. pumila color genotype produces a
unique amount of Hsp70 and antioxidative pigments in both the vegetative and
reproductive plant parts, in order to protect cellular homeostasis under fluctuating
temperature conditions prevailing in its population.
PB  - Beograd: University, Faculty of Biology
C3  - Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia
T1  - Flower color morphs of Iris pumila differ in the amount of heat shock protein 70 and pigments with antioxidant properties
SP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6204
ER  - 

@conference{
author = "Hočevar, Katarina and Nolić, Vladimir and Budečević, Sanja and Manitašević Jovanović, Sanja",
year = "2017",
abstract = "Introduction: Natural populations of the dwarf bearded iris, Iris pumila, are markedly
polymorphic for flower color. Selection pressures exerted by a range of abiotic and
biotic factors could be involved in maintaining the polymorphism. Here we
quantified the amount of the Hsp70 and the two groups of pigments with
antioxidative properties: anthocyanins and carotenoids.These molecules impact
abiotic stress tolerance, ultimately influencing the fitness of individual plants.
Methods: A total of fifty genotypes raised in a common-garden and belonging to a
corresponding color class (yellow, blue, violet, dark blue and dark violet) were
surveyed. One fully developed leaf and a flower from each genotype were
analyzed for the Hsp70 amount and pigments concentration.
Results: The Western blot analysis revealed the presence of one isoform for the Hsp70
in leaf and two isoforms in flower organs. In both vegetative and reproductive tissues
the amount of Hsp70 was found to be the lowest in yellow colored genotypes
compared to other color classes. In violet and blue flowers, the concentration of
Hsp70 decreased gradually from light to dark colored variants. Conversely, the
concentration of anthocyanins was found to be higher in darker than in the lighter
color morphs. An inverse trend was observed for the total carotenoids
concentration.
Conclusion: This study revealed that each I. pumila color genotype produces a
unique amount of Hsp70 and antioxidative pigments in both the vegetative and
reproductive plant parts, in order to protect cellular homeostasis under fluctuating
temperature conditions prevailing in its population.",
publisher = "Beograd: University, Faculty of Biology",
journal = "Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia",
title = "Flower color morphs of Iris pumila differ in the amount of heat shock protein 70 and pigments with antioxidant properties",
pages = "39",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6204"
}

Hočevar, K., Nolić, V., Budečević, S.,& Manitašević Jovanović, S.. (2017). Flower color morphs of Iris pumila differ in the amount of heat shock protein 70 and pigments with antioxidant properties. in Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia
Beograd: University, Faculty of Biology., 39.
https://hdl.handle.net/21.15107/rcub_ibiss_6204

Hočevar K, Nolić V, Budečević S, Manitašević Jovanović S. Flower color morphs of Iris pumila differ in the amount of heat shock protein 70 and pigments with antioxidant properties. in Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia. 2017;:39.
https://hdl.handle.net/21.15107/rcub_ibiss_6204 .

Hočevar, Katarina, Nolić, Vladimir, Budečević, Sanja, Manitašević Jovanović, Sanja, "Flower color morphs of Iris pumila differ in the amount of heat shock protein 70 and pigments with antioxidant properties" in Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia (2017):39,
https://hdl.handle.net/21.15107/rcub_ibiss_6204 .

TY  - JOUR
AU  - Radović, Sanja
AU  - Urošević, Aleksandar
AU  - Hočevar, Katarina
AU  - Vuleta, Ana
AU  - Manitašević Jovanović, Sanja
AU  - Tucić, Branka
PY  - 2017
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641600086R
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/838
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2772
AB  - The Iris flower is a complex morphological structure composed of two trimerous whorls of functionally distinct petaloid organs (the falls and the standards), one whorl of the stamens and one tricarpellary gynoecium. The petal-like style arms of the carpels are banded over the basal part of the falls, forming three pollination tunnels, each of which is perceived by the Iris pollinators as a single bilaterally symmetrical flower. Apart from the stamens, all petaloid floral organs are preferentially involved in advertising rewards to potential pollinators. Here we used the methods of geometric morphometrics to explore the shape variation in falls, standards and style arms of the Iris pumila flowers and to disentangle the symmetric and the asymmetric component of the total shape variance. Our results show that symmetric variation contributes mostly to the total shape variance in each of the three floral organs. Fluctuating asymmetry (FA) was the dominant component of the asymmetric shape variation in the falls and the standards, but appeared to be marginally significant in the style arms. The values of FA indexes for the shape of falls (insects' landing platforms) and for the shape of standards (long-distance reward signals) were found to be two orders of magnitude greater compared to that of the style arms. Directional asymmetry appeared to be very low, but highly statistically significant for all analyzed floral organs. Because floral symmetry can reliably indicate the presence of floral rewards, an almost perfect symmetry recorded for the style arm shape might be the outcome of pollinator preferences for symmetrical pollination units.
T2  - Archives of Biological Sciences
T1  - Geometric morphometrics of functionally distinct floral organs in Iris pumila: Analyzing patterns of symmetric and asymmetric shape variations
IS  - 2
VL  - 69
DO  - 10.2298/ABS160912086R
SP  - 223
EP  - 231
ER  - 

@article{
author = "Radović, Sanja and Urošević, Aleksandar and Hočevar, Katarina and Vuleta, Ana and Manitašević Jovanović, Sanja and Tucić, Branka",
year = "2017",
abstract = "The Iris flower is a complex morphological structure composed of two trimerous whorls of functionally distinct petaloid organs (the falls and the standards), one whorl of the stamens and one tricarpellary gynoecium. The petal-like style arms of the carpels are banded over the basal part of the falls, forming three pollination tunnels, each of which is perceived by the Iris pollinators as a single bilaterally symmetrical flower. Apart from the stamens, all petaloid floral organs are preferentially involved in advertising rewards to potential pollinators. Here we used the methods of geometric morphometrics to explore the shape variation in falls, standards and style arms of the Iris pumila flowers and to disentangle the symmetric and the asymmetric component of the total shape variance. Our results show that symmetric variation contributes mostly to the total shape variance in each of the three floral organs. Fluctuating asymmetry (FA) was the dominant component of the asymmetric shape variation in the falls and the standards, but appeared to be marginally significant in the style arms. The values of FA indexes for the shape of falls (insects' landing platforms) and for the shape of standards (long-distance reward signals) were found to be two orders of magnitude greater compared to that of the style arms. Directional asymmetry appeared to be very low, but highly statistically significant for all analyzed floral organs. Because floral symmetry can reliably indicate the presence of floral rewards, an almost perfect symmetry recorded for the style arm shape might be the outcome of pollinator preferences for symmetrical pollination units.",
journal = "Archives of Biological Sciences",
title = "Geometric morphometrics of functionally distinct floral organs in Iris pumila: Analyzing patterns of symmetric and asymmetric shape variations",
number = "2",
volume = "69",
doi = "10.2298/ABS160912086R",
pages = "223-231"
}

Radović, S., Urošević, A., Hočevar, K., Vuleta, A., Manitašević Jovanović, S.,& Tucić, B.. (2017). Geometric morphometrics of functionally distinct floral organs in Iris pumila: Analyzing patterns of symmetric and asymmetric shape variations. in Archives of Biological Sciences, 69(2), 223-231.
https://doi.org/10.2298/ABS160912086R

Radović S, Urošević A, Hočevar K, Vuleta A, Manitašević Jovanović S, Tucić B. Geometric morphometrics of functionally distinct floral organs in Iris pumila: Analyzing patterns of symmetric and asymmetric shape variations. in Archives of Biological Sciences. 2017;69(2):223-231.
doi:10.2298/ABS160912086R .

Radović, Sanja, Urošević, Aleksandar, Hočevar, Katarina, Vuleta, Ana, Manitašević Jovanović, Sanja, Tucić, Branka, "Geometric morphometrics of functionally distinct floral organs in Iris pumila: Analyzing patterns of symmetric and asymmetric shape variations" in Archives of Biological Sciences, 69, no. 2 (2017):223-231,
https://doi.org/10.2298/ABS160912086R . .

TY  - THES
AU  - Đorđević, Mirko
PY  - 2017
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2556
AB  - Ključna pretpostavka evolucione teorije starenja jeste da opadanje uzrasno-specifičnog preživljavanja organizama predstavlja rezultat smanjenja intenziteta prirodne selekcije nakon početka reprodukcije. U osnovi evolucije starenja nalaze se dva populaciono-genetička mehanizma: akumulacija mutacija (AM) i antagonistička plejotropija (AP). U skladu sa pretpostavkama evolucione teorije starenja, kod eksperimentalnih populacija pasuljevog žiška (Acanthoscelides obtectus) selektovanih više od 170 generacija za ranu (E režim) ili kasnu (L režim) reprodukciju, uočena je divergencija u dužini života i drugim preadultnim i adultnim osobinama životne istorije. Evolucija veće dužine života u L režimu u saglasnosti je sa pretpostavkama AP modela, dok u evoluciji kraćeg života E žižaka važnu ulogu ima akumulacija mutacija. Tretiranjem E i L žižaka pesticidima (parakvat i tebufenpirad) koji generišu slobodne radikale, potvrđena je pozitivna korelacija između dužine života i otpornosti na oksidativni stres. Budući da mitohondrija predstavlja glavno mesto sinteze energije i slobodnih radikala, u ovoj tezi je istraženo da li je uzrasno-specifična selekcija oblikovala varijabilnost mtDNK, nDNK i njihovih epistatičkih interakcija. Genotipizacijom mitohondrijskog COI gena i mikrosatelitskih lokusa pokazana je efikasnost uzrasno-specifične selekcije u međupopulacionoj divergenciji mitohondrijskog i jedarnog genskog pula kod pasuljevog žiška. Odvijanje procesa mito-jedarne koevolucije analizirano je korišćenjem mito-jedarnih linija u kojima su E i L mitohondrijski genomi kombinovani sa E i L jedarnim genomima. Pokazano je da su linije sa narušenim mito-jedarnim interakcijama imale niže aktivnosti mitohondrijskih kompleksa elektron transportnog lanca i lošiju preadultnu performansu.
AB  - According to the evolutionary theory of ageing, age-specific decline in survival is the result of decreasing intensity of natural selection after the onset of reproduction. The evolution of ageing may be explained by two different, but not mutually exclusive, genetic mechanisms: antagonistic pleiotropy (AP) and mutation accumulation (MA). In accordance with evolutionary theory of ageing, two sets of the seed beetle (Acanthoscelides obtectus) experimental lines selected for more than 170 generations for early (E regime) or late (L regime) reproduction, show divergence in longevity and ageing patterns, as well as in other preadult and adult life history traits. Evolution of long life in L regime is predominantly based on antagonistic pleiotropy gene effects, while mutation accumulation plays important role in evolution of short life in E regime. By exposure of E and L beetles to two pesticides (paraquat and tebufenpyrad) that generate free radicals, it was confirmed that extended longevity in L beetles (especially in females) was associated with higher oxidative stress resistance. Additionally, since mitochondria are main site of free radical and energy production, effects of age-specific laboratory selection on mtDNA, nDNA and their epistatic interaction were assessed. Genotyping of mitochondrial COI gene and nuclear microsatellite loci showed that selection in E and L regimes led to divergent evolution of mtDNA and nDNA gene pools. Mitonuclear coevolution was investigated using mitonuclear introgression lines in which E and L mitochondrial genomes were expressed in both E and L nuclear background. It was found that lines with disrupted mitonuclear interactions had lower activity of the mitochondrial electron transport chain complexes and lower preadult performance.
PB  - Belgrade: University of Belgrade, Faculty of Biology
T1  - Evolucija starenja kod laboratorijskih populacija Acanthoscelides obtectus: uloga mitohondrija i oksidativnog stresa
T1  - Evolution of ageing in laboratory populations of Acanthoscelides obtectus: role of mitochondria and oxidative stress
SP  - 1
EP  - 218
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_2556
ER  - 

@phdthesis{
author = "Đorđević, Mirko",
year = "2017",
abstract = "Ključna pretpostavka evolucione teorije starenja jeste da opadanje uzrasno-specifičnog preživljavanja organizama predstavlja rezultat smanjenja intenziteta prirodne selekcije nakon početka reprodukcije. U osnovi evolucije starenja nalaze se dva populaciono-genetička mehanizma: akumulacija mutacija (AM) i antagonistička plejotropija (AP). U skladu sa pretpostavkama evolucione teorije starenja, kod eksperimentalnih populacija pasuljevog žiška (Acanthoscelides obtectus) selektovanih više od 170 generacija za ranu (E režim) ili kasnu (L režim) reprodukciju, uočena je divergencija u dužini života i drugim preadultnim i adultnim osobinama životne istorije. Evolucija veće dužine života u L režimu u saglasnosti je sa pretpostavkama AP modela, dok u evoluciji kraćeg života E žižaka važnu ulogu ima akumulacija mutacija. Tretiranjem E i L žižaka pesticidima (parakvat i tebufenpirad) koji generišu slobodne radikale, potvrđena je pozitivna korelacija između dužine života i otpornosti na oksidativni stres. Budući da mitohondrija predstavlja glavno mesto sinteze energije i slobodnih radikala, u ovoj tezi je istraženo da li je uzrasno-specifična selekcija oblikovala varijabilnost mtDNK, nDNK i njihovih epistatičkih interakcija. Genotipizacijom mitohondrijskog COI gena i mikrosatelitskih lokusa pokazana je efikasnost uzrasno-specifične selekcije u međupopulacionoj divergenciji mitohondrijskog i jedarnog genskog pula kod pasuljevog žiška. Odvijanje procesa mito-jedarne koevolucije analizirano je korišćenjem mito-jedarnih linija u kojima su E i L mitohondrijski genomi kombinovani sa E i L jedarnim genomima. Pokazano je da su linije sa narušenim mito-jedarnim interakcijama imale niže aktivnosti mitohondrijskih kompleksa elektron transportnog lanca i lošiju preadultnu performansu., According to the evolutionary theory of ageing, age-specific decline in survival is the result of decreasing intensity of natural selection after the onset of reproduction. The evolution of ageing may be explained by two different, but not mutually exclusive, genetic mechanisms: antagonistic pleiotropy (AP) and mutation accumulation (MA). In accordance with evolutionary theory of ageing, two sets of the seed beetle (Acanthoscelides obtectus) experimental lines selected for more than 170 generations for early (E regime) or late (L regime) reproduction, show divergence in longevity and ageing patterns, as well as in other preadult and adult life history traits. Evolution of long life in L regime is predominantly based on antagonistic pleiotropy gene effects, while mutation accumulation plays important role in evolution of short life in E regime. By exposure of E and L beetles to two pesticides (paraquat and tebufenpyrad) that generate free radicals, it was confirmed that extended longevity in L beetles (especially in females) was associated with higher oxidative stress resistance. Additionally, since mitochondria are main site of free radical and energy production, effects of age-specific laboratory selection on mtDNA, nDNA and their epistatic interaction were assessed. Genotyping of mitochondrial COI gene and nuclear microsatellite loci showed that selection in E and L regimes led to divergent evolution of mtDNA and nDNA gene pools. Mitonuclear coevolution was investigated using mitonuclear introgression lines in which E and L mitochondrial genomes were expressed in both E and L nuclear background. It was found that lines with disrupted mitonuclear interactions had lower activity of the mitochondrial electron transport chain complexes and lower preadult performance.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
title = "Evolucija starenja kod laboratorijskih populacija Acanthoscelides obtectus: uloga mitohondrija i oksidativnog stresa, Evolution of ageing in laboratory populations of Acanthoscelides obtectus: role of mitochondria and oxidative stress",
pages = "1-218",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_2556"
}

Đorđević, M.. (2017). Evolucija starenja kod laboratorijskih populacija Acanthoscelides obtectus: uloga mitohondrija i oksidativnog stresa. 
Belgrade: University of Belgrade, Faculty of Biology., 1-218.
https://hdl.handle.net/21.15107/rcub_ibiss_2556

Đorđević M. Evolucija starenja kod laboratorijskih populacija Acanthoscelides obtectus: uloga mitohondrija i oksidativnog stresa. 2017;:1-218.
https://hdl.handle.net/21.15107/rcub_ibiss_2556 .

Đorđević, Mirko, "Evolucija starenja kod laboratorijskih populacija Acanthoscelides obtectus: uloga mitohondrija i oksidativnog stresa" (2017):1-218,
https://hdl.handle.net/21.15107/rcub_ibiss_2556 .

TY  - JOUR
AU  - Anđelković, Marko
AU  - Tomović, Ljiljana
AU  - Ivanović, Ana
PY  - 2017
UR  - http://doi.wiley.com/10.1111/jzo.12477
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2776
AB  - Morphological integration, the covariation among phenotypic traits generated by common development and function, has been in the scope of evolutionary research for decades. As a morphological structure with complex development and various functions, the cranial skeleton represents a particularly interesting model for studies on morphological integration. However, most of the empirical investigations were done on akinetic and compact cranial skeletons of mammals. Here, we explore the pattern of integration in the extremely kinetic cranial skeleton of two closely related snake species, Natrix natrix and N. tessellata (Natricinae, Colubridae). In snakes, elements of jaws and palates on the left and right side are not spatially connected or firmly fused, allowing independent motion. Spatial independence of skeletal elements on the left and right side and their functional interconnections with extreme kinetic abilities, provide unique feeding performance in this group of tetrapods. By comparing patterns of symmetric and asymmetric components of variation we analysed cavariation patterns between kinetic and akinetic cranial elements. We tested whether the functionally and spatially connected bones are more integrated than disconnected ones and we examine impact of development and function on the morphological integration. We also explored whether and how allometry affects morphological integration in the snake's skull. Using micro-CT scanning 3D geometric morphometrics we showed strong covariation between the braincase and elements of the feeding apparatus, and that spatially disconnected elements are not more integrated than the connected ones. We also showed that function is the main factor that generates the pattern of morphological integration, because the signal of developmental integration is very weak and probably masked by strong functional integration of skeletal elements. Allometry has a significant impact on the morphological integration, by increasing integration of the skull, particularly integration of the lower jaw bones (compound and dentary), prefrontal, palatine and quadrate with the other skeletal elements. © 2017 The Zoological Society of London.
T2  - Journal of Zoology
T1  - Morphological integration of the kinetic skull in Natrix snakes
DO  - 10.1111/jzo.12477
ER  - 

@article{
author = "Anđelković, Marko and Tomović, Ljiljana and Ivanović, Ana",
year = "2017",
abstract = "Morphological integration, the covariation among phenotypic traits generated by common development and function, has been in the scope of evolutionary research for decades. As a morphological structure with complex development and various functions, the cranial skeleton represents a particularly interesting model for studies on morphological integration. However, most of the empirical investigations were done on akinetic and compact cranial skeletons of mammals. Here, we explore the pattern of integration in the extremely kinetic cranial skeleton of two closely related snake species, Natrix natrix and N. tessellata (Natricinae, Colubridae). In snakes, elements of jaws and palates on the left and right side are not spatially connected or firmly fused, allowing independent motion. Spatial independence of skeletal elements on the left and right side and their functional interconnections with extreme kinetic abilities, provide unique feeding performance in this group of tetrapods. By comparing patterns of symmetric and asymmetric components of variation we analysed cavariation patterns between kinetic and akinetic cranial elements. We tested whether the functionally and spatially connected bones are more integrated than disconnected ones and we examine impact of development and function on the morphological integration. We also explored whether and how allometry affects morphological integration in the snake's skull. Using micro-CT scanning 3D geometric morphometrics we showed strong covariation between the braincase and elements of the feeding apparatus, and that spatially disconnected elements are not more integrated than the connected ones. We also showed that function is the main factor that generates the pattern of morphological integration, because the signal of developmental integration is very weak and probably masked by strong functional integration of skeletal elements. Allometry has a significant impact on the morphological integration, by increasing integration of the skull, particularly integration of the lower jaw bones (compound and dentary), prefrontal, palatine and quadrate with the other skeletal elements. © 2017 The Zoological Society of London.",
journal = "Journal of Zoology",
title = "Morphological integration of the kinetic skull in Natrix snakes",
doi = "10.1111/jzo.12477"
}

Anđelković, M., Tomović, L.,& Ivanović, A.. (2017). Morphological integration of the kinetic skull in Natrix snakes. in Journal of Zoology.
https://doi.org/10.1111/jzo.12477

Anđelković M, Tomović L, Ivanović A. Morphological integration of the kinetic skull in Natrix snakes. in Journal of Zoology. 2017;.
doi:10.1111/jzo.12477 .

Anđelković, Marko, Tomović, Ljiljana, Ivanović, Ana, "Morphological integration of the kinetic skull in Natrix snakes" in Journal of Zoology (2017),
https://doi.org/10.1111/jzo.12477 . .

TY  - JOUR
AU  - Arnqvist, Göran
AU  - Stojković, Biljana
AU  - Rönn, Johanna L.
AU  - Immonen, Elina
AU  - White, Craig
PY  - 2017
UR  - http://doi.wiley.com/10.1111/1365-2435.12927
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2820
AB  - Metabolic rate (MR) is a key functional trait simply because metabolism converts resources into population growth rate. Yet, our empirical understanding of the sources of within species variation in MR, as well as of its life history and ecological correlates, is rather limited. Here, we assess whether MR lies at the root of a syndrome of correlated rate-dependent life-history traits in an insect. Selection for early (E) or late (L) age-at-reproduction for > 160 generations in the bean beetle Acanthoscelides obtectus has produced beetles that differ markedly in juvenile development, body size, fecundity schedules, ageing and life span. Here, we use micro-respirometry to test whether this has been associated with the evolution of age- and sex-specific metabolic phenotypes. We find that mass-specific MR is 18% higher in E lines compared to L lines and that MR decreases more rapidly with chronological, but not biological, age in E lines. Males, under sexual selection to "live-fast-die-young", show 50% higher MR than females and MR decreased more rapidly with age in males. Our results are consistent with a central role for MR for the divergence in "pace-of-life" seen in these beetles, supporting the view that MR lies at the root of ecologically relevant life-history trait variation within species.
T2  - Functional Ecology
T1  - The pace-of-life: A sex-specific link between metabolic rate and life history in bean beetles
DO  - 10.1111/1365-2435.12927
ER  - 

@article{
author = "Arnqvist, Göran and Stojković, Biljana and Rönn, Johanna L. and Immonen, Elina and White, Craig",
year = "2017",
abstract = "Metabolic rate (MR) is a key functional trait simply because metabolism converts resources into population growth rate. Yet, our empirical understanding of the sources of within species variation in MR, as well as of its life history and ecological correlates, is rather limited. Here, we assess whether MR lies at the root of a syndrome of correlated rate-dependent life-history traits in an insect. Selection for early (E) or late (L) age-at-reproduction for > 160 generations in the bean beetle Acanthoscelides obtectus has produced beetles that differ markedly in juvenile development, body size, fecundity schedules, ageing and life span. Here, we use micro-respirometry to test whether this has been associated with the evolution of age- and sex-specific metabolic phenotypes. We find that mass-specific MR is 18% higher in E lines compared to L lines and that MR decreases more rapidly with chronological, but not biological, age in E lines. Males, under sexual selection to "live-fast-die-young", show 50% higher MR than females and MR decreased more rapidly with age in males. Our results are consistent with a central role for MR for the divergence in "pace-of-life" seen in these beetles, supporting the view that MR lies at the root of ecologically relevant life-history trait variation within species.",
journal = "Functional Ecology",
title = "The pace-of-life: A sex-specific link between metabolic rate and life history in bean beetles",
doi = "10.1111/1365-2435.12927"
}

Arnqvist, G., Stojković, B., Rönn, J. L., Immonen, E.,& White, C.. (2017). The pace-of-life: A sex-specific link between metabolic rate and life history in bean beetles. in Functional Ecology.
https://doi.org/10.1111/1365-2435.12927

Arnqvist G, Stojković B, Rönn JL, Immonen E, White C. The pace-of-life: A sex-specific link between metabolic rate and life history in bean beetles. in Functional Ecology. 2017;.
doi:10.1111/1365-2435.12927 .

Arnqvist, Göran, Stojković, Biljana, Rönn, Johanna L., Immonen, Elina, White, Craig, "The pace-of-life: A sex-specific link between metabolic rate and life history in bean beetles" in Functional Ecology (2017),
https://doi.org/10.1111/1365-2435.12927 . .

TY  - JOUR
AU  - Stojković, Biljana
AU  - Sayadi, Ahmed
AU  - Đorđević, Mirko
AU  - Jović, Jelena
AU  - Savković, Uroš
AU  - Arnqvist, Göran
PY  - 2017
UR  - http://doi.wiley.com/10.1111/evo.13102
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2550
AB  - Mitochondria play a key role in ageing. The pursuit of genes that regulate variation in life span and ageing have shown that several nuclear-encoded mitochondrial genes are important. However, the role of mitochondrial encoded genes (mtDNA) is more controversial and our appreciation of the role of mtDNA for the evolution of life span is limited. We use replicated lines of seed beetles that have been artificially selected for long or short life for >190 generations, now showing dramatic phenotypic differences, to test for a possible role of mtDNA in the divergent evolution of ageing and life span. We show that these divergent selection regimes led to the evolution of significantly different mtDNA haplotype frequencies. Selection for a long life and late reproduction generated positive selection for one specific haplotype, which was fixed in most such lines. In contrast, selection for reproduction early in life led to both positive selection as well as negative frequency-dependent selection on two different haplotypes, which were both present in all such lines. Our findings suggest that the evolution of life span was in part mediated by mtDNA, providing support for the emerging general tenet that adaptive evolution of life-history syndromes may involve mtDNA.
T2  - Evolution
T1  - Divergent evolution of life span associated with mitochondrial DNA evolution
IS  - 1
VL  - 71
DO  - 10.1111/evo.13102
SP  - 160
EP  - 166
ER  - 

@article{
author = "Stojković, Biljana and Sayadi, Ahmed and Đorđević, Mirko and Jović, Jelena and Savković, Uroš and Arnqvist, Göran",
year = "2017",
abstract = "Mitochondria play a key role in ageing. The pursuit of genes that regulate variation in life span and ageing have shown that several nuclear-encoded mitochondrial genes are important. However, the role of mitochondrial encoded genes (mtDNA) is more controversial and our appreciation of the role of mtDNA for the evolution of life span is limited. We use replicated lines of seed beetles that have been artificially selected for long or short life for >190 generations, now showing dramatic phenotypic differences, to test for a possible role of mtDNA in the divergent evolution of ageing and life span. We show that these divergent selection regimes led to the evolution of significantly different mtDNA haplotype frequencies. Selection for a long life and late reproduction generated positive selection for one specific haplotype, which was fixed in most such lines. In contrast, selection for reproduction early in life led to both positive selection as well as negative frequency-dependent selection on two different haplotypes, which were both present in all such lines. Our findings suggest that the evolution of life span was in part mediated by mtDNA, providing support for the emerging general tenet that adaptive evolution of life-history syndromes may involve mtDNA.",
journal = "Evolution",
title = "Divergent evolution of life span associated with mitochondrial DNA evolution",
number = "1",
volume = "71",
doi = "10.1111/evo.13102",
pages = "160-166"
}

Stojković, B., Sayadi, A., Đorđević, M., Jović, J., Savković, U.,& Arnqvist, G.. (2017). Divergent evolution of life span associated with mitochondrial DNA evolution. in Evolution, 71(1), 160-166.
https://doi.org/10.1111/evo.13102

Stojković B, Sayadi A, Đorđević M, Jović J, Savković U, Arnqvist G. Divergent evolution of life span associated with mitochondrial DNA evolution. in Evolution. 2017;71(1):160-166.
doi:10.1111/evo.13102 .

Stojković, Biljana, Sayadi, Ahmed, Đorđević, Mirko, Jović, Jelena, Savković, Uroš, Arnqvist, Göran, "Divergent evolution of life span associated with mitochondrial DNA evolution" in Evolution, 71, no. 1 (2017):160-166,
https://doi.org/10.1111/evo.13102 . .

TY  - JOUR
AU  - Đorđević, Mirko
AU  - Stojković, Biljana
AU  - Savković, Uroš
AU  - Immonen, Elina
AU  - Tucić, Nikola
AU  - Lazarević, Jelica
AU  - Arnqvist, Göran
PY  - 2017
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2600
AB  - The role of mitochondrial DNA for the evolution of life-history traits remains debated. We examined mitonuclear effects on the activity of the multisubunit complex of the electron transport chain (ETC) involved in oxidative phosphorylation (OXPHOS) across lines of the seed beetle Acanthoscelides obtectus selected for a short (E) or a long (L) life for more than >160 generations. We constructed and phenotyped mitonuclear introgression lines, which allowed us to assess the independent effects of the evolutionary history of the nuclear and the mitochondrial genome. The nuclear genome was responsible for the largest share of divergence seen in ageing. However, the mitochondrial genome also had sizeable effects, which were sex-specific and expressed primarily as epistatic interactions with the nuclear genome. The effects of mitonuclear disruption were largely consistent with mitonuclear coadaptation. Variation in ETC activity explained a large proportion of variance in ageing and life-history traits and this multivariate relationship differed somewhat between the sexes. In conclusion, mitonuclear epistasis has played an important role in the laboratory evolution of ETC complex activity, ageing, and life histories and these are closely associated. The mitonuclear architecture of evolved differences in life-history traits and mitochondrial bioenergetics was sex-specific.
T2  - Evolution
T1  - Sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles
IS  - 2
VL  - 71
DO  - 10.1111/evo.13109
SP  - 274
EP  - 288
ER  - 

@article{
author = "Đorđević, Mirko and Stojković, Biljana and Savković, Uroš and Immonen, Elina and Tucić, Nikola and Lazarević, Jelica and Arnqvist, Göran",
year = "2017",
abstract = "The role of mitochondrial DNA for the evolution of life-history traits remains debated. We examined mitonuclear effects on the activity of the multisubunit complex of the electron transport chain (ETC) involved in oxidative phosphorylation (OXPHOS) across lines of the seed beetle Acanthoscelides obtectus selected for a short (E) or a long (L) life for more than >160 generations. We constructed and phenotyped mitonuclear introgression lines, which allowed us to assess the independent effects of the evolutionary history of the nuclear and the mitochondrial genome. The nuclear genome was responsible for the largest share of divergence seen in ageing. However, the mitochondrial genome also had sizeable effects, which were sex-specific and expressed primarily as epistatic interactions with the nuclear genome. The effects of mitonuclear disruption were largely consistent with mitonuclear coadaptation. Variation in ETC activity explained a large proportion of variance in ageing and life-history traits and this multivariate relationship differed somewhat between the sexes. In conclusion, mitonuclear epistasis has played an important role in the laboratory evolution of ETC complex activity, ageing, and life histories and these are closely associated. The mitonuclear architecture of evolved differences in life-history traits and mitochondrial bioenergetics was sex-specific.",
journal = "Evolution",
title = "Sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles",
number = "2",
volume = "71",
doi = "10.1111/evo.13109",
pages = "274-288"
}

Đorđević, M., Stojković, B., Savković, U., Immonen, E., Tucić, N., Lazarević, J.,& Arnqvist, G.. (2017). Sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles. in Evolution, 71(2), 274-288.
https://doi.org/10.1111/evo.13109

Đorđević M, Stojković B, Savković U, Immonen E, Tucić N, Lazarević J, Arnqvist G. Sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles. in Evolution. 2017;71(2):274-288.
doi:10.1111/evo.13109 .

Đorđević, Mirko, Stojković, Biljana, Savković, Uroš, Immonen, Elina, Tucić, Nikola, Lazarević, Jelica, Arnqvist, Göran, "Sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles" in Evolution, 71, no. 2 (2017):274-288,
https://doi.org/10.1111/evo.13109 . .

TY  - THES
AU  - Anđelković, Marko
PY  - 2017
UR  - http://uvidok.rcub.bg.ac.rs/handle/123456789/2185
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2880
AB  - Većina dosadašnjih istraživanja strukture i obrazaca varijabilnosti glavenog skeleta
odnosila su se na grupe sa akinetičkom lobanjom. Istraživanje kinetizma u okviru
glavenog skeleta zmija može doprineti boljem razumevanju odnosa razvojne i
funkcijske integracije, uticaja alometrije na varijabilnost oblika i morfološku integraciju
glavenog skeleta, promena oblika tokom ontogenije, kao i morfološke divergencije
između polova. Pored toga, analizama varijabilnosti pileusa može se steći uvid u opštu
sliku varijabilnosti glavenog regiona. Osnovni zadatak ove disertacije je utvrđivanje
obrazaca morfološke varijabilnosti i faktora koji utiču na varijabilnost glavenog skeleta
i pileusa kod dve sestrinske vrste zmija iz roda Natrix (belouške, N. natrix i ribarice, N.
tessellata) primenom metoda kompjuterizovane tomografije i geometrijske
morfometrije. Rezultati su pokazali da se belouška i ribarica razlikuju u veličini i obliku
elemenata glavenog skeleta. Uočene razlike najverovatnije odslikavaju razlike u tipu i
veličini plena, sredini u kojoj love, strategijama lova i sposobnostima držanja i gutanja
plena. Polni dimorfizam u veličini i obliku elemenata glavenog skeleta veoma je izražen
kod belouške i ribarice. Kod obe vrste moždana čaura i elementi trofičkog aparata su
visoko integrisani, dok se kosti njuške ponašaju kao zasebni moduli. Funkcija najviše
doprinosi integrisanosti elemenata glavenog skeleta. Utvrđeno je da se tek rođene
belouške i ribarice razlikuju u obliku pileusa, dok se unutar vrsta polni dimorfizam u
obliku pileusa ispoljava tek kod adulata. Između moždane čaure i pileusa ustanovljeni
su visoki nivoi korelisanosti, kako za veličinu, tako i za oblik.
AB  - Most of the previous investigations on the structure and patterns of cranial skeletons
variability were done in groups with akinetic skull. Research of snake’s kinetic cranial
skeleton can provide a better understanding of the relationship between developmental
and functional integration, influence of allometry on shape variation and morphological
integration of the cranial skeleton, shape changes during ontogeny, as well as
morphological divergence between the sexes. In addition, analysis of pileus variability
may provide insight into general pattern of head variability. The main aim of this thesis
was to define the patterns of morphological variability and factors that affect the
variability of head skeleton and pileus in two sister snakes species from the genus
Natrix (Grass snake, N. natrix and Dice snake, N. tessellata) using the methods of
computed tomography and geometric morphometrics. The results showed that N. natrix
and N. tessellata differ in size and shape of cranial elements. Observed differences
probably reflect differences in the type and size of the prey, hunting environment and
strategies, handling abilities and prey swallowing. Sexual dimorphism in size and shape
of cranial elements are pronounced among Grass and Dice snakes. In both species,
braincase and elements of the trophic apparatus are highly integrated, while the bones of
the snout can be considered as separate modules. The function is the main factor of
morphological integration between cranial elements. Hatchlings of Grass and Dice
snakes differed in pileus shape, while sexual dimorphism in pileus shape was detected
in adults only. For both size and shape, high levels of correlation between braincase and
pileus were estimated.
PB  - Belgrade: University of Belgrade, Faculty of Biology
T2  - University of Belgrade, Faculty of Biology
T1  - Morfološka varijabilnost kinetičkog glavenog skeleta i pileusa zmija iz roda Natrix (N. natrix i N. tessellata)
T1  - Morphological variation of kinetic head skeleton and pileus in snake from the genus
Natrix (N. natrix and N. tessellata)
SP  - 1
EP  - 107
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_2880
ER  - 

@phdthesis{
author = "Anđelković, Marko",
year = "2017",
abstract = "Većina dosadašnjih istraživanja strukture i obrazaca varijabilnosti glavenog skeleta
odnosila su se na grupe sa akinetičkom lobanjom. Istraživanje kinetizma u okviru
glavenog skeleta zmija može doprineti boljem razumevanju odnosa razvojne i
funkcijske integracije, uticaja alometrije na varijabilnost oblika i morfološku integraciju
glavenog skeleta, promena oblika tokom ontogenije, kao i morfološke divergencije
između polova. Pored toga, analizama varijabilnosti pileusa može se steći uvid u opštu
sliku varijabilnosti glavenog regiona. Osnovni zadatak ove disertacije je utvrđivanje
obrazaca morfološke varijabilnosti i faktora koji utiču na varijabilnost glavenog skeleta
i pileusa kod dve sestrinske vrste zmija iz roda Natrix (belouške, N. natrix i ribarice, N.
tessellata) primenom metoda kompjuterizovane tomografije i geometrijske
morfometrije. Rezultati su pokazali da se belouška i ribarica razlikuju u veličini i obliku
elemenata glavenog skeleta. Uočene razlike najverovatnije odslikavaju razlike u tipu i
veličini plena, sredini u kojoj love, strategijama lova i sposobnostima držanja i gutanja
plena. Polni dimorfizam u veličini i obliku elemenata glavenog skeleta veoma je izražen
kod belouške i ribarice. Kod obe vrste moždana čaura i elementi trofičkog aparata su
visoko integrisani, dok se kosti njuške ponašaju kao zasebni moduli. Funkcija najviše
doprinosi integrisanosti elemenata glavenog skeleta. Utvrđeno je da se tek rođene
belouške i ribarice razlikuju u obliku pileusa, dok se unutar vrsta polni dimorfizam u
obliku pileusa ispoljava tek kod adulata. Između moždane čaure i pileusa ustanovljeni
su visoki nivoi korelisanosti, kako za veličinu, tako i za oblik., Most of the previous investigations on the structure and patterns of cranial skeletons
variability were done in groups with akinetic skull. Research of snake’s kinetic cranial
skeleton can provide a better understanding of the relationship between developmental
and functional integration, influence of allometry on shape variation and morphological
integration of the cranial skeleton, shape changes during ontogeny, as well as
morphological divergence between the sexes. In addition, analysis of pileus variability
may provide insight into general pattern of head variability. The main aim of this thesis
was to define the patterns of morphological variability and factors that affect the
variability of head skeleton and pileus in two sister snakes species from the genus
Natrix (Grass snake, N. natrix and Dice snake, N. tessellata) using the methods of
computed tomography and geometric morphometrics. The results showed that N. natrix
and N. tessellata differ in size and shape of cranial elements. Observed differences
probably reflect differences in the type and size of the prey, hunting environment and
strategies, handling abilities and prey swallowing. Sexual dimorphism in size and shape
of cranial elements are pronounced among Grass and Dice snakes. In both species,
braincase and elements of the trophic apparatus are highly integrated, while the bones of
the snout can be considered as separate modules. The function is the main factor of
morphological integration between cranial elements. Hatchlings of Grass and Dice
snakes differed in pileus shape, while sexual dimorphism in pileus shape was detected
in adults only. For both size and shape, high levels of correlation between braincase and
pileus were estimated.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "University of Belgrade, Faculty of Biology",
title = "Morfološka varijabilnost kinetičkog glavenog skeleta i pileusa zmija iz roda Natrix (N. natrix i N. tessellata), Morphological variation of kinetic head skeleton and pileus in snake from the genus
Natrix (N. natrix and N. tessellata)",
pages = "1-107",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_2880"
}

Anđelković, M.. (2017). Morfološka varijabilnost kinetičkog glavenog skeleta i pileusa zmija iz roda Natrix (N. natrix i N. tessellata). in University of Belgrade, Faculty of Biology
Belgrade: University of Belgrade, Faculty of Biology., 1-107.
https://hdl.handle.net/21.15107/rcub_ibiss_2880

Anđelković M. Morfološka varijabilnost kinetičkog glavenog skeleta i pileusa zmija iz roda Natrix (N. natrix i N. tessellata). in University of Belgrade, Faculty of Biology. 2017;:1-107.
https://hdl.handle.net/21.15107/rcub_ibiss_2880 .

Anđelković, Marko, "Morfološka varijabilnost kinetičkog glavenog skeleta i pileusa zmija iz roda Natrix (N. natrix i N. tessellata)" in University of Belgrade, Faculty of Biology (2017):1-107,
https://hdl.handle.net/21.15107/rcub_ibiss_2880 .

TY  - JOUR
AU  - Vuleta, Ana
AU  - Manitašević Jovanović, Sanja
AU  - Tucić, Branka
PY  - 2016
UR  - http://www.ncbi.nlm.nih.gov/pubmed/26841194
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0981942816300109
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2597
AB  - High solar radiation has been recognized as one of the main causes of the overproduction of reactive oxygen species (ROS) and oxidative stress in plants. To remove the excess of ROS, plants use different antioxidants and tune their activity and/or isoform number as required for given light conditions. In this study, the adaptiveness of light-induced variation in the activities and isoform patterns of key enzymatic antioxidants SOD, APX and CAT was tested in leaves of Iris pumila clonal plants from two natural populations inhabiting a sun exposed dune site and a forest understory, using a reciprocal-transplant experiment. At the exposed habitat, the mean enzymatic activity of total SODs was significantly greater than that in the shaded one, while the amount of the mitochondrial MnSOD was notably higher compared to the plastidic Cu/ZnSOD. However, the number of Cu/ZnSOD isoforms was greater in the forest understory relative to the exposed site (three vs. two, respectively). An inverse relationship recorded between the quantities of MnSOD and Cu/ZnSOD in alternative light habitats might indicate that the two enzymes compensate each other in maintaining intracellular ROS and redox balance. The adaptive population differentiation in APX activity was exclusively recorded in the open habitat, which indicated that the synergistic effect of high light and temperature stress could be the principal selective factor, rather than high light alone. The enzymatic activity of CAT was similar between the two populations, implicating APX as the primary H2O2 scavenger in the I. pumila leaves exposed to high light intensity.
T2  - Plant Physiology and Biochemistry
T1  - Adaptive flexibility of enzymatic antioxidants SOD, APX and CAT to high light stress: The clonal perennial monocot Iris pumila as a study case
VL  - 100
DO  - 10.1016/j.plaphy.2016.01.011
SP  - 166
EP  - 173
ER  - 

@article{
author = "Vuleta, Ana and Manitašević Jovanović, Sanja and Tucić, Branka",
year = "2016",
abstract = "High solar radiation has been recognized as one of the main causes of the overproduction of reactive oxygen species (ROS) and oxidative stress in plants. To remove the excess of ROS, plants use different antioxidants and tune their activity and/or isoform number as required for given light conditions. In this study, the adaptiveness of light-induced variation in the activities and isoform patterns of key enzymatic antioxidants SOD, APX and CAT was tested in leaves of Iris pumila clonal plants from two natural populations inhabiting a sun exposed dune site and a forest understory, using a reciprocal-transplant experiment. At the exposed habitat, the mean enzymatic activity of total SODs was significantly greater than that in the shaded one, while the amount of the mitochondrial MnSOD was notably higher compared to the plastidic Cu/ZnSOD. However, the number of Cu/ZnSOD isoforms was greater in the forest understory relative to the exposed site (three vs. two, respectively). An inverse relationship recorded between the quantities of MnSOD and Cu/ZnSOD in alternative light habitats might indicate that the two enzymes compensate each other in maintaining intracellular ROS and redox balance. The adaptive population differentiation in APX activity was exclusively recorded in the open habitat, which indicated that the synergistic effect of high light and temperature stress could be the principal selective factor, rather than high light alone. The enzymatic activity of CAT was similar between the two populations, implicating APX as the primary H2O2 scavenger in the I. pumila leaves exposed to high light intensity.",
journal = "Plant Physiology and Biochemistry",
title = "Adaptive flexibility of enzymatic antioxidants SOD, APX and CAT to high light stress: The clonal perennial monocot Iris pumila as a study case",
volume = "100",
doi = "10.1016/j.plaphy.2016.01.011",
pages = "166-173"
}

Vuleta, A., Manitašević Jovanović, S.,& Tucić, B.. (2016). Adaptive flexibility of enzymatic antioxidants SOD, APX and CAT to high light stress: The clonal perennial monocot Iris pumila as a study case. in Plant Physiology and Biochemistry, 100, 166-173.
https://doi.org/10.1016/j.plaphy.2016.01.011

Vuleta A, Manitašević Jovanović S, Tucić B. Adaptive flexibility of enzymatic antioxidants SOD, APX and CAT to high light stress: The clonal perennial monocot Iris pumila as a study case. in Plant Physiology and Biochemistry. 2016;100:166-173.
doi:10.1016/j.plaphy.2016.01.011 .

Vuleta, Ana, Manitašević Jovanović, Sanja, Tucić, Branka, "Adaptive flexibility of enzymatic antioxidants SOD, APX and CAT to high light stress: The clonal perennial monocot Iris pumila as a study case" in Plant Physiology and Biochemistry, 100 (2016):166-173,
https://doi.org/10.1016/j.plaphy.2016.01.011 . .

TY  - JOUR
AU  - Đorđević, Mirko
AU  - Stojković, Biljana
AU  - Savković, Uroš
AU  - Immonen, Elina
AU  - Tucić, Nikola
AU  - Lazarević, Jelica
AU  - Arnqvist, Göran
PY  - 2016
UR  - http://doi.wiley.com/10.1111/evo.13109
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2549
AB  - The role of mitochondrial DNA for the evolution of life-history traits remains debated. We examined mitonuclear effects on the activity of the multisubunit complex of the electron transport chain (ETC) involved in oxidative phosphorylation (OXPHOS) across lines of the seed beetle Acanthoscelides obtectus selected for a short (E) or a long (L) life for more than >160 generations. We constructed and phenotyped mitonuclear introgression lines, which allowed us to assess the independent effects of the evolutionary history of the nuclear and the mitochondrial genome. The nuclear genome was responsible for the largest share of divergence seen in ageing. However, the mitochondrial genome also had sizeable effects, which were sex-specific and expressed primarily as epistatic interactions with the nuclear genome. The effects of mitonuclear disruption were largely consistent with mitonuclear coadaptation. Variation in ETC activity explained a large proportion of variance in ageing and life-history traits and this multivariate relationship differed somewhat between the sexes. In conclusion, mitonuclear epistasis has played an important role in the laboratory evolution of ETC complex activity, ageing, and life histories and these are closely associated. The mitonuclear architecture of evolved differences in life-history traits and mitochondrial bioenergetics was sex-specific.
T2  - Evolution
T1  - Sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles
DO  - 10.1111/evo.13109
ER  - 

@article{
author = "Đorđević, Mirko and Stojković, Biljana and Savković, Uroš and Immonen, Elina and Tucić, Nikola and Lazarević, Jelica and Arnqvist, Göran",
year = "2016",
abstract = "The role of mitochondrial DNA for the evolution of life-history traits remains debated. We examined mitonuclear effects on the activity of the multisubunit complex of the electron transport chain (ETC) involved in oxidative phosphorylation (OXPHOS) across lines of the seed beetle Acanthoscelides obtectus selected for a short (E) or a long (L) life for more than >160 generations. We constructed and phenotyped mitonuclear introgression lines, which allowed us to assess the independent effects of the evolutionary history of the nuclear and the mitochondrial genome. The nuclear genome was responsible for the largest share of divergence seen in ageing. However, the mitochondrial genome also had sizeable effects, which were sex-specific and expressed primarily as epistatic interactions with the nuclear genome. The effects of mitonuclear disruption were largely consistent with mitonuclear coadaptation. Variation in ETC activity explained a large proportion of variance in ageing and life-history traits and this multivariate relationship differed somewhat between the sexes. In conclusion, mitonuclear epistasis has played an important role in the laboratory evolution of ETC complex activity, ageing, and life histories and these are closely associated. The mitonuclear architecture of evolved differences in life-history traits and mitochondrial bioenergetics was sex-specific.",
journal = "Evolution",
title = "Sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles",
doi = "10.1111/evo.13109"
}

Đorđević, M., Stojković, B., Savković, U., Immonen, E., Tucić, N., Lazarević, J.,& Arnqvist, G.. (2016). Sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles. in Evolution.
https://doi.org/10.1111/evo.13109

Đorđević M, Stojković B, Savković U, Immonen E, Tucić N, Lazarević J, Arnqvist G. Sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles. in Evolution. 2016;.
doi:10.1111/evo.13109 .

Đorđević, Mirko, Stojković, Biljana, Savković, Uroš, Immonen, Elina, Tucić, Nikola, Lazarević, Jelica, Arnqvist, Göran, "Sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles" in Evolution (2016),
https://doi.org/10.1111/evo.13109 . .

TY  - JOUR
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Šešlija Jovanović, Darka
AU  - Lazarević, Jelica
AU  - Tucić, Nikola
AU  - Stojković, Biljana
PY  - 2016
UR  - http://onlinelibrary.wiley.com/doi/10.1111/jeb.12831/abstract
UR  - http://www.ncbi.nlm.nih.gov/pubmed/26790127
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2925
AB  - Expansion of the host range in phytophagous insects depends on their ability to form an association with a novel plant through changes in host-related traits. Phenotypic plasticity has important effects on initial survival of individuals faced with a new plant, as well as on the courses of evolutionary change during long-term adaptation to novel conditions. Using experimental populations of the seed beetle that evolved on ancestral (common bean) or novel (chickpea) host and applying reciprocal transplant at both larval and adult stage on the alternative host plant, we studied the relationship between the initial (plastic) phases of host-shift and the subsequent stages of evolutionary divergence in life-history strategies between populations exposed to the host-shift process. After 48 generations, populations became well adapted to chickpea by evolving the life-history strategy with prolonged larval development, increased body mass, earlier reproduction, shorter lifespan and decreased plasticity of all traits compared with ancestral conditions. In chickpea-adapted beetles, negative fitness consequences of low plasticity of pre-adult development (revealed as severe decrease in egg-to-adult viability on beans) exhibited mismatch with positive effects of low plasticity (i.e. low host sensitivity) in oviposition and fecundity. In contrast, beetles adapted to the ancestral host showed high plasticity of developmental process, which enabled high larval survival on chickpea, whereas elevated plasticity in adult behaviour (i.e. high host sensitivity) resulted in delayed reproduction and decreased fecundity on chickpea. The analysis of population growth parameters revealed significant fluctuation during successive phases of the host-shift process in A. obtectus.
T2  - Journal of Evolutionary Biology
T1  - Experimentally induced host-shift changes life-history strategy in a seed beetle.
IS  - 4
VL  - 29
DO  - 10.1111/jeb.12831
SP  - 837
EP  - 47
ER  - 

@article{
author = "Savković, Uroš and Đorđević, Mirko and Šešlija Jovanović, Darka and Lazarević, Jelica and Tucić, Nikola and Stojković, Biljana",
year = "2016",
abstract = "Expansion of the host range in phytophagous insects depends on their ability to form an association with a novel plant through changes in host-related traits. Phenotypic plasticity has important effects on initial survival of individuals faced with a new plant, as well as on the courses of evolutionary change during long-term adaptation to novel conditions. Using experimental populations of the seed beetle that evolved on ancestral (common bean) or novel (chickpea) host and applying reciprocal transplant at both larval and adult stage on the alternative host plant, we studied the relationship between the initial (plastic) phases of host-shift and the subsequent stages of evolutionary divergence in life-history strategies between populations exposed to the host-shift process. After 48 generations, populations became well adapted to chickpea by evolving the life-history strategy with prolonged larval development, increased body mass, earlier reproduction, shorter lifespan and decreased plasticity of all traits compared with ancestral conditions. In chickpea-adapted beetles, negative fitness consequences of low plasticity of pre-adult development (revealed as severe decrease in egg-to-adult viability on beans) exhibited mismatch with positive effects of low plasticity (i.e. low host sensitivity) in oviposition and fecundity. In contrast, beetles adapted to the ancestral host showed high plasticity of developmental process, which enabled high larval survival on chickpea, whereas elevated plasticity in adult behaviour (i.e. high host sensitivity) resulted in delayed reproduction and decreased fecundity on chickpea. The analysis of population growth parameters revealed significant fluctuation during successive phases of the host-shift process in A. obtectus.",
journal = "Journal of Evolutionary Biology",
title = "Experimentally induced host-shift changes life-history strategy in a seed beetle.",
number = "4",
volume = "29",
doi = "10.1111/jeb.12831",
pages = "837-47"
}

Savković, U., Đorđević, M., Šešlija Jovanović, D., Lazarević, J., Tucić, N.,& Stojković, B.. (2016). Experimentally induced host-shift changes life-history strategy in a seed beetle.. in Journal of Evolutionary Biology, 29(4), 837-47.
https://doi.org/10.1111/jeb.12831

Savković U, Đorđević M, Šešlija Jovanović D, Lazarević J, Tucić N, Stojković B. Experimentally induced host-shift changes life-history strategy in a seed beetle.. in Journal of Evolutionary Biology. 2016;29(4):837-47.
doi:10.1111/jeb.12831 .

Savković, Uroš, Đorđević, Mirko, Šešlija Jovanović, Darka, Lazarević, Jelica, Tucić, Nikola, Stojković, Biljana, "Experimentally induced host-shift changes life-history strategy in a seed beetle." in Journal of Evolutionary Biology, 29, no. 4 (2016):837-47,
https://doi.org/10.1111/jeb.12831 . .

TY  - THES
AU  - Savković, Uroš
PY  - 2016
UR  - http://uvidok.rcub.bg.ac.rs/handle/123456789/1564
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2514
PB  - Belgrade: University of Belgrade, Faculty of Biology
T2  - University of Belgrade, Faculty of Biology
T1  - Uloga fenotipske plastičnosti osobina životne istorije i ponašanja u procesima specijacije Acanthoscelides obtectus
T1  - The role of phenotypic plasticity of life history
and behavioural traits during speciation
processes in Acanthoscelides obtectus
SP  - 1
EP  - 228
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_2514
ER  - 

@phdthesis{
author = "Savković, Uroš",
year = "2016",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "University of Belgrade, Faculty of Biology",
title = "Uloga fenotipske plastičnosti osobina životne istorije i ponašanja u procesima specijacije Acanthoscelides obtectus, The role of phenotypic plasticity of life history
and behavioural traits during speciation
processes in Acanthoscelides obtectus",
pages = "1-228",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_2514"
}

Savković, U.. (2016). Uloga fenotipske plastičnosti osobina životne istorije i ponašanja u procesima specijacije Acanthoscelides obtectus. in University of Belgrade, Faculty of Biology
Belgrade: University of Belgrade, Faculty of Biology., 1-228.
https://hdl.handle.net/21.15107/rcub_ibiss_2514

Savković U. Uloga fenotipske plastičnosti osobina životne istorije i ponašanja u procesima specijacije Acanthoscelides obtectus. in University of Belgrade, Faculty of Biology. 2016;:1-228.
https://hdl.handle.net/21.15107/rcub_ibiss_2514 .

Savković, Uroš, "Uloga fenotipske plastičnosti osobina životne istorije i ponašanja u procesima specijacije Acanthoscelides obtectus" in University of Belgrade, Faculty of Biology (2016):1-228,
https://hdl.handle.net/21.15107/rcub_ibiss_2514 .