TY  - JOUR
AU  - Vujotić, Ljiljana
AU  - Matić, Siniša
AU  - Borković Mitić, Slavica
AU  - Stojsavljević, Aleksandar
AU  - Mutić, Jelena
AU  - Baščarević, Vladimir
AU  - Joković, Miloš
AU  - Pavlović, Slađan
PY  - 2020
UR  - https://www.termedia.pl/Association-between-oxidative-stress-biomarkers-and-concentrations-of-some-metal-ions-in-the-blood-of-patients-with-brain-tumors-and-hydrocephalus,19,37458,1,1.html
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3459
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3713
AB  - IntroductionAny substance that induces production of free radicals can be a potential cause of brain damage. The aim of our study was to investigate the relationship between some metal ions and oxidative stress biomarkers in the blood of patients with brain tumor and hydrocephalus.Material and methodsOur study included 27 control subjects, 24 patients with brain tumor and 21 patients with hydrocephalus. The activities of superoxide dismutase (CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), glutathione S-transferase (GST) and acetyl cholinesterase (AChE), as well as concentrations of reduced glutathione (GSH), lipid peroxides (TBARS) and sulfhydryl groups (SH) were analyzed in the plasma and red blood cells (RBCs) of patients. We also determined the concentrations of Mn, Ni, Co, Cu, Zn, As, Se, Cd, Hg and Fe.ResultsThe higher activity of SOD and concentration of GSH in both investigated groups could indicate higher oxidative stress. We also observed decreased levels of SH groups in both groups of patients. In both groups of patients we detected decreased concentrations of Ni, Co, Zn and Fe (and Mn in brain tumor patients) and increased concentrations of As, Se and Cd in the blood. Interestingly, we observed a higher concentration of Cd in both plasma and RBCs of hydrocephalus patients compared to the patients with brain tumor.ConclusionsThere are strong correlations between some metal ion concentrations and certain oxidative stress biomarkers in the blood of patients, which supports our hypothesis, but the observed trend needs to be further investigated.
PB  - Termedia & Banach
T2  - Archives of Medical Science
T1  - Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus
IS  - 4
VL  - 16
DO  - 10.5114/aoms.2019.87409
SP  - 811
EP  - 819
ER  - 

@article{
author = "Vujotić, Ljiljana and Matić, Siniša and Borković Mitić, Slavica and Stojsavljević, Aleksandar and Mutić, Jelena and Baščarević, Vladimir and Joković, Miloš and Pavlović, Slađan",
year = "2020",
abstract = "IntroductionAny substance that induces production of free radicals can be a potential cause of brain damage. The aim of our study was to investigate the relationship between some metal ions and oxidative stress biomarkers in the blood of patients with brain tumor and hydrocephalus.Material and methodsOur study included 27 control subjects, 24 patients with brain tumor and 21 patients with hydrocephalus. The activities of superoxide dismutase (CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), glutathione S-transferase (GST) and acetyl cholinesterase (AChE), as well as concentrations of reduced glutathione (GSH), lipid peroxides (TBARS) and sulfhydryl groups (SH) were analyzed in the plasma and red blood cells (RBCs) of patients. We also determined the concentrations of Mn, Ni, Co, Cu, Zn, As, Se, Cd, Hg and Fe.ResultsThe higher activity of SOD and concentration of GSH in both investigated groups could indicate higher oxidative stress. We also observed decreased levels of SH groups in both groups of patients. In both groups of patients we detected decreased concentrations of Ni, Co, Zn and Fe (and Mn in brain tumor patients) and increased concentrations of As, Se and Cd in the blood. Interestingly, we observed a higher concentration of Cd in both plasma and RBCs of hydrocephalus patients compared to the patients with brain tumor.ConclusionsThere are strong correlations between some metal ion concentrations and certain oxidative stress biomarkers in the blood of patients, which supports our hypothesis, but the observed trend needs to be further investigated.",
publisher = "Termedia & Banach",
journal = "Archives of Medical Science",
title = "Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus",
number = "4",
volume = "16",
doi = "10.5114/aoms.2019.87409",
pages = "811-819"
}

Vujotić, L., Matić, S., Borković Mitić, S., Stojsavljević, A., Mutić, J., Baščarević, V., Joković, M.,& Pavlović, S.. (2020). Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus. in Archives of Medical Science
Termedia & Banach., 16(4), 811-819.
https://doi.org/10.5114/aoms.2019.87409

Vujotić L, Matić S, Borković Mitić S, Stojsavljević A, Mutić J, Baščarević V, Joković M, Pavlović S. Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus. in Archives of Medical Science. 2020;16(4):811-819.
doi:10.5114/aoms.2019.87409 .

Vujotić, Ljiljana, Matić, Siniša, Borković Mitić, Slavica, Stojsavljević, Aleksandar, Mutić, Jelena, Baščarević, Vladimir, Joković, Miloš, Pavlović, Slađan, "Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus" in Archives of Medical Science, 16, no. 4 (2020):811-819,
https://doi.org/10.5114/aoms.2019.87409 . .

TY  - JOUR
AU  - Stikić, Radmila I.
AU  - Milinčić, Danijel D.
AU  - Kostić, Aleksandar Ž.
AU  - Jovanović, Zorica B.
AU  - Gašić, Uroš
AU  - Tešić, Živoslav Lj.
AU  - Djordjević, Nataša Z.
AU  - Savić, Sladjana K.
AU  - Czekus, Borisz G.
AU  - Pešić, Mirjana
PY  - 2020
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1002/cche.10278
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3639
AB  - Quinoa is considered to be a “natural functional food” due to a lot of bioactive compounds that are beneficial for human health by helping prevent the risks of different diseases. The aim of this study was to test the health‐promoting characteristics of Puno and Titicaca quinoa seeds grown in Serbia by assessing their phenolic and flavonoid content, antioxidant activities, and potential anticancer effect on human colorectal cancer cells. 13 phenolic compounds were identified and quantified in the seeds of both cultivars, but their profile and concentration of individual phenolic compounds differed. Cytotoxic activities were present in both cultivars, whereas the pronounced concentration, and time‐dependent effects were more expressed in Puno extracts. The extracts of the seeds of both cultivars are a rich source of phenolic and flavonoid compounds and with high antioxidant activities. Potent anticancer activity against the human colorectal cancer was expressed in both investigated cultivars. The study confirmed the health benefit potential of Puno and Titicaca quinoa cultivars, grown in Serbia. These results are the first to demonstrate a potent anticancer activity of quinoa seed extract against the human colorectal cancer cell line HCT‐116, as well as the presence of 7 new phenolic and flavonoid compounds.
T2  - Cereal Chemistry
T1  - Polyphenolic profiles, antioxidant and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars
IS  - 3
VL  - 97
DO  - 10.1002/cche.10278
SP  - cche.10278
SP  - 626
EP  - 633
ER  - 

@article{
author = "Stikić, Radmila I. and Milinčić, Danijel D. and Kostić, Aleksandar Ž. and Jovanović, Zorica B. and Gašić, Uroš and Tešić, Živoslav Lj. and Djordjević, Nataša Z. and Savić, Sladjana K. and Czekus, Borisz G. and Pešić, Mirjana",
year = "2020",
abstract = "Quinoa is considered to be a “natural functional food” due to a lot of bioactive compounds that are beneficial for human health by helping prevent the risks of different diseases. The aim of this study was to test the health‐promoting characteristics of Puno and Titicaca quinoa seeds grown in Serbia by assessing their phenolic and flavonoid content, antioxidant activities, and potential anticancer effect on human colorectal cancer cells. 13 phenolic compounds were identified and quantified in the seeds of both cultivars, but their profile and concentration of individual phenolic compounds differed. Cytotoxic activities were present in both cultivars, whereas the pronounced concentration, and time‐dependent effects were more expressed in Puno extracts. The extracts of the seeds of both cultivars are a rich source of phenolic and flavonoid compounds and with high antioxidant activities. Potent anticancer activity against the human colorectal cancer was expressed in both investigated cultivars. The study confirmed the health benefit potential of Puno and Titicaca quinoa cultivars, grown in Serbia. These results are the first to demonstrate a potent anticancer activity of quinoa seed extract against the human colorectal cancer cell line HCT‐116, as well as the presence of 7 new phenolic and flavonoid compounds.",
journal = "Cereal Chemistry",
title = "Polyphenolic profiles, antioxidant and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars",
number = "3",
volume = "97",
doi = "10.1002/cche.10278",
pages = "cche.10278-626-633"
}

Stikić, R. I., Milinčić, D. D., Kostić, A. Ž., Jovanović, Z. B., Gašić, U., Tešić, Ž. Lj., Djordjević, N. Z., Savić, S. K., Czekus, B. G.,& Pešić, M.. (2020). Polyphenolic profiles, antioxidant and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars. in Cereal Chemistry, 97(3), cche.10278-633.
https://doi.org/10.1002/cche.10278

Stikić RI, Milinčić DD, Kostić AŽ, Jovanović ZB, Gašić U, Tešić ŽL, Djordjević NZ, Savić SK, Czekus BG, Pešić M. Polyphenolic profiles, antioxidant and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars. in Cereal Chemistry. 2020;97(3):cche.10278-633.
doi:10.1002/cche.10278 .

Stikić, Radmila I., Milinčić, Danijel D., Kostić, Aleksandar Ž., Jovanović, Zorica B., Gašić, Uroš, Tešić, Živoslav Lj., Djordjević, Nataša Z., Savić, Sladjana K., Czekus, Borisz G., Pešić, Mirjana, "Polyphenolic profiles, antioxidant and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars" in Cereal Chemistry, 97, no. 3 (2020):cche.10278-633,
https://doi.org/10.1002/cche.10278 . .

TY  - JOUR
AU  - Gavrilović, Branka
AU  - Prokić, Marko
AU  - Petrović, Tamara
AU  - Despotović, Svetlana
AU  - Radovanović, Tijana
AU  - Krizmanić, Imre
AU  - Ćirić, Miloš
AU  - Gavrić, Jelena
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3794
AB  - There is little information in scientific literature as to how conditions created by a microcystin (MC) producing cyanobacterial bloom affect the oxidant/antioxidant, biotransformation and neurotoxicity parameters in adult frogs in situ. We investigated biochemical parameters in the skin and muscle of Pelophylax kl. esculentus from Lake Ludaš (Serbia) by comparing frogs that live on the northern bloom side (BS) of the lake with those that inhabit the southern no-bloom side (NBS). A higher protein carbonylation level and lower antioxidant defense system capability in the skin of frogs living in conditions of the cyanobacterial bloom were observed. Inhibition of glutathione-dependent machinery was the major mechanism responsible for the induction of cyanobacterial bloom-mediated oxidative stress in frog skin. On the other hand, the detected higher ability of muscle to overcome bloom prooxidant toxicity was linked to a higher efficiency of the biotransformation system through glutathione-S-transferase activity and/or was the consequence of indirect exposure of the tissue to the bloom. Our results have also revealed that the cyanobacterial bloom conditions induced the cholinergic neurotransmitter system in both tissues. This study provides a better understanding of the ecotoxicological impact of the MC producing cyanobacterial bloom on frogs in situ. However, further investigations of the complex mechanism involved in cyanobacterial bloom toxicity in real environmental conditions are required.
PB  - Elsevier B.V.
T2  - Aquatic Toxicology
T1  - Biochemical parameters in skin and muscle of Pelophylax kl. esculentus frogs: Influence of a cyanobacterial bloom in situ
VL  - 220
DO  - 10.1016/j.aquatox.2019.105399
SP  - 105399
ER  - 

@article{
author = "Gavrilović, Branka and Prokić, Marko and Petrović, Tamara and Despotović, Svetlana and Radovanović, Tijana and Krizmanić, Imre and Ćirić, Miloš and Gavrić, Jelena",
year = "2020",
abstract = "There is little information in scientific literature as to how conditions created by a microcystin (MC) producing cyanobacterial bloom affect the oxidant/antioxidant, biotransformation and neurotoxicity parameters in adult frogs in situ. We investigated biochemical parameters in the skin and muscle of Pelophylax kl. esculentus from Lake Ludaš (Serbia) by comparing frogs that live on the northern bloom side (BS) of the lake with those that inhabit the southern no-bloom side (NBS). A higher protein carbonylation level and lower antioxidant defense system capability in the skin of frogs living in conditions of the cyanobacterial bloom were observed. Inhibition of glutathione-dependent machinery was the major mechanism responsible for the induction of cyanobacterial bloom-mediated oxidative stress in frog skin. On the other hand, the detected higher ability of muscle to overcome bloom prooxidant toxicity was linked to a higher efficiency of the biotransformation system through glutathione-S-transferase activity and/or was the consequence of indirect exposure of the tissue to the bloom. Our results have also revealed that the cyanobacterial bloom conditions induced the cholinergic neurotransmitter system in both tissues. This study provides a better understanding of the ecotoxicological impact of the MC producing cyanobacterial bloom on frogs in situ. However, further investigations of the complex mechanism involved in cyanobacterial bloom toxicity in real environmental conditions are required.",
publisher = "Elsevier B.V.",
journal = "Aquatic Toxicology",
title = "Biochemical parameters in skin and muscle of Pelophylax kl. esculentus frogs: Influence of a cyanobacterial bloom in situ",
volume = "220",
doi = "10.1016/j.aquatox.2019.105399",
pages = "105399"
}

Gavrilović, B., Prokić, M., Petrović, T., Despotović, S., Radovanović, T., Krizmanić, I., Ćirić, M.,& Gavrić, J.. (2020). Biochemical parameters in skin and muscle of Pelophylax kl. esculentus frogs: Influence of a cyanobacterial bloom in situ. in Aquatic Toxicology
Elsevier B.V.., 220, 105399.
https://doi.org/10.1016/j.aquatox.2019.105399

Gavrilović B, Prokić M, Petrović T, Despotović S, Radovanović T, Krizmanić I, Ćirić M, Gavrić J. Biochemical parameters in skin and muscle of Pelophylax kl. esculentus frogs: Influence of a cyanobacterial bloom in situ. in Aquatic Toxicology. 2020;220:105399.
doi:10.1016/j.aquatox.2019.105399 .

Gavrilović, Branka, Prokić, Marko, Petrović, Tamara, Despotović, Svetlana, Radovanović, Tijana, Krizmanić, Imre, Ćirić, Miloš, Gavrić, Jelena, "Biochemical parameters in skin and muscle of Pelophylax kl. esculentus frogs: Influence of a cyanobacterial bloom in situ" in Aquatic Toxicology, 220 (2020):105399,
https://doi.org/10.1016/j.aquatox.2019.105399 . .

TY  - JOUR
AU  - Đurašević, Siniša
AU  - Stojković, Maja
AU  - Bogdanović, Ljiljana
AU  - Pavlović, Slađan
AU  - Borković Mitić, Slavica
AU  - Grigorov, Ilijana
AU  - Bogojević, Desanka
AU  - Jasnić, Nebojša
AU  - Tosti, Tomislav
AU  - Đurović, Saša
AU  - Đorđević, Jelena
AU  - Todorović, Zoran
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3519
AB  - Acute renal ischemia/reperfusion (I/R) injury is a clinical condition that is challenging to
treat. Meldonium is an anti-ischemic agent that shifts energy production from fatty acid oxidation
to less oxygen-consuming glycolysis. Thus, in this study we investigated the effects of a four-week
meldonium pre-treatment (300 mg/kg b.m./day) on acute renal I/R in male rats (Wistar strain). Our
results showed that meldonium decreased animal body mass gain, food and water intake, and
carnitine, glucose, and lactic acid kidney content. In kidneys of animals subjected to I/R, meldonium
increased phosphorylation of mitogen-activated protein kinase p38 and protein kinase B, and
increased the expression of nuclear factor erythroid 2-related factor 2 and haeme oxygenase 1,
causing manganese superoxide dismutase expression and activity to increase, as well as lipid
peroxidation, cooper-zinc superoxide dismutase, glutathione peroxidase, and glutathione reductase
activities to decrease. By decreasing the kidney Bax/Bcl2 expression ratio and kidney and serum
high mobility group box 1 protein content, meldonium reduced apoptotic and necrotic events in
I/R, as confirmed by kidney histology. Meldonium increased adrenal noradrenaline content and
serum, adrenal, hepatic, and renal ascorbic/dehydroascorbic acid ratio, which caused complex
changes in renal lipidomics. Taken together, our results have confirmed that meldonium pretreatment protects against I/R-induced oxidative stress and apoptosis/necrosis.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - The effects of meldonium on the renal acute ischemia/reperfusion injury in rats
IS  - 22
VL  - 20
DO  - 10.3390/ijms20225747
SP  - 5747
ER  - 

@article{
author = "Đurašević, Siniša and Stojković, Maja and Bogdanović, Ljiljana and Pavlović, Slađan and Borković Mitić, Slavica and Grigorov, Ilijana and Bogojević, Desanka and Jasnić, Nebojša and Tosti, Tomislav and Đurović, Saša and Đorđević, Jelena and Todorović, Zoran",
year = "2019",
abstract = "Acute renal ischemia/reperfusion (I/R) injury is a clinical condition that is challenging to
treat. Meldonium is an anti-ischemic agent that shifts energy production from fatty acid oxidation
to less oxygen-consuming glycolysis. Thus, in this study we investigated the effects of a four-week
meldonium pre-treatment (300 mg/kg b.m./day) on acute renal I/R in male rats (Wistar strain). Our
results showed that meldonium decreased animal body mass gain, food and water intake, and
carnitine, glucose, and lactic acid kidney content. In kidneys of animals subjected to I/R, meldonium
increased phosphorylation of mitogen-activated protein kinase p38 and protein kinase B, and
increased the expression of nuclear factor erythroid 2-related factor 2 and haeme oxygenase 1,
causing manganese superoxide dismutase expression and activity to increase, as well as lipid
peroxidation, cooper-zinc superoxide dismutase, glutathione peroxidase, and glutathione reductase
activities to decrease. By decreasing the kidney Bax/Bcl2 expression ratio and kidney and serum
high mobility group box 1 protein content, meldonium reduced apoptotic and necrotic events in
I/R, as confirmed by kidney histology. Meldonium increased adrenal noradrenaline content and
serum, adrenal, hepatic, and renal ascorbic/dehydroascorbic acid ratio, which caused complex
changes in renal lipidomics. Taken together, our results have confirmed that meldonium pretreatment protects against I/R-induced oxidative stress and apoptosis/necrosis.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "The effects of meldonium on the renal acute ischemia/reperfusion injury in rats",
number = "22",
volume = "20",
doi = "10.3390/ijms20225747",
pages = "5747"
}

Đurašević, S., Stojković, M., Bogdanović, L., Pavlović, S., Borković Mitić, S., Grigorov, I., Bogojević, D., Jasnić, N., Tosti, T., Đurović, S., Đorđević, J.,& Todorović, Z.. (2019). The effects of meldonium on the renal acute ischemia/reperfusion injury in rats. in International Journal of Molecular Sciences
MDPI., 20(22), 5747.
https://doi.org/10.3390/ijms20225747

Đurašević S, Stojković M, Bogdanović L, Pavlović S, Borković Mitić S, Grigorov I, Bogojević D, Jasnić N, Tosti T, Đurović S, Đorđević J, Todorović Z. The effects of meldonium on the renal acute ischemia/reperfusion injury in rats. in International Journal of Molecular Sciences. 2019;20(22):5747.
doi:10.3390/ijms20225747 .

Đurašević, Siniša, Stojković, Maja, Bogdanović, Ljiljana, Pavlović, Slađan, Borković Mitić, Slavica, Grigorov, Ilijana, Bogojević, Desanka, Jasnić, Nebojša, Tosti, Tomislav, Đurović, Saša, Đorđević, Jelena, Todorović, Zoran, "The effects of meldonium on the renal acute ischemia/reperfusion injury in rats" in International Journal of Molecular Sciences, 20, no. 22 (2019):5747,
https://doi.org/10.3390/ijms20225747 . .

TY  - JOUR
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Prokić, Marko
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Anđelković, Marko
AU  - Tomović, Ljiljana
AU  - Borković Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Saičić, Zorica
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S1532045619300924?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3351
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3365
AB  - In this study we examined possible differences in heavy metal accumulation and oxidative stress parameters in the liver and muscle of two semi-aquatic snakes: grass snake (Natrix natrix) and dice snake (N. tessellata), that inhabit the same environment but differ in prey diversity. The obtained results revealed some interspecies, inter-tissue, prey-snake and prey-prey differences in heavy metal concentrations. Grass snakes pray contained significantly higher concentrations of Al, Cr and Fe as compared to food of dice snakes. Both investigated snakes accumulated generally lower concentrations of metals than their prey, indicating that they are not at risk of contaminant biomagnification. A significant interspecies difference in accumulation was observed only for Cu and Mn concentrations. On the other hand, analysis of oxidative stress biomarkers showed clear differences between the investigated snake species and the two investigated tissues. The liver of grass snake had increased superoxide dismutase, glutathione reductase and glutathione-S-transferase activities in comparison to dice snake. In muscle, a reverse trend was observed for the activities of these three enzymes, as well as for glutathione peroxidase activity. The higher number of significant correlations observed between oxidative stress biomarkers and heavy metal concentrations in grass snake points to upregulation of the antioxidative system (AOS), which resulted in a lower TBARS concentration. Results show that while the investigated snake species did not differ significantly in the accumulated metals, their defense mechanisms were different. This reveals the complexity of the AOS and points to the cooperation of different AOS components in individuals from natural populations.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?
VL  - 223
DO  - 10.1016/J.CBPC.2019.05.010
SP  - 26
EP  - 34
ER  - 

@article{
author = "Gavrić, Jelena and Despotović, Svetlana and Prokić, Marko and Gavrilović, Branka and Radovanović, Tijana and Anđelković, Marko and Tomović, Ljiljana and Borković Mitić, Slavica and Pavlović, Slađan and Saičić, Zorica",
year = "2019",
abstract = "In this study we examined possible differences in heavy metal accumulation and oxidative stress parameters in the liver and muscle of two semi-aquatic snakes: grass snake (Natrix natrix) and dice snake (N. tessellata), that inhabit the same environment but differ in prey diversity. The obtained results revealed some interspecies, inter-tissue, prey-snake and prey-prey differences in heavy metal concentrations. Grass snakes pray contained significantly higher concentrations of Al, Cr and Fe as compared to food of dice snakes. Both investigated snakes accumulated generally lower concentrations of metals than their prey, indicating that they are not at risk of contaminant biomagnification. A significant interspecies difference in accumulation was observed only for Cu and Mn concentrations. On the other hand, analysis of oxidative stress biomarkers showed clear differences between the investigated snake species and the two investigated tissues. The liver of grass snake had increased superoxide dismutase, glutathione reductase and glutathione-S-transferase activities in comparison to dice snake. In muscle, a reverse trend was observed for the activities of these three enzymes, as well as for glutathione peroxidase activity. The higher number of significant correlations observed between oxidative stress biomarkers and heavy metal concentrations in grass snake points to upregulation of the antioxidative system (AOS), which resulted in a lower TBARS concentration. Results show that while the investigated snake species did not differ significantly in the accumulated metals, their defense mechanisms were different. This reveals the complexity of the AOS and points to the cooperation of different AOS components in individuals from natural populations.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?",
volume = "223",
doi = "10.1016/J.CBPC.2019.05.010",
pages = "26-34"
}

Gavrić, J., Despotović, S., Prokić, M., Gavrilović, B., Radovanović, T., Anđelković, M., Tomović, L., Borković Mitić, S., Pavlović, S.,& Saičić, Z.. (2019). Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 223, 26-34.
https://doi.org/10.1016/J.CBPC.2019.05.010

Gavrić J, Despotović S, Prokić M, Gavrilović B, Radovanović T, Anđelković M, Tomović L, Borković Mitić S, Pavlović S, Saičić Z. Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2019;223:26-34.
doi:10.1016/J.CBPC.2019.05.010 .

Gavrić, Jelena, Despotović, Svetlana, Prokić, Marko, Gavrilović, Branka, Radovanović, Tijana, Anđelković, Marko, Tomović, Ljiljana, Borković Mitić, Slavica, Pavlović, Slađan, Saičić, Zorica, "Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?" in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 223 (2019):26-34,
https://doi.org/10.1016/J.CBPC.2019.05.010 . .

TY  - JOUR
AU  - Matić, Miloš M.
AU  - Paunović, Milica G.
AU  - Milošević, Marija D.
AU  - Ognjanović, Branka I.
AU  - Saičić, Zorica
PY  - 2019
UR  - https://www.tandfonline.com/doi/full/10.1080/01480545.2019.1587451
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3309
AB  - Acetaminophen is widely used as an over-the-counter analgesic and antipyretic drug. The aim of the present study was to investigate the pro-oxidative effects of acetaminophen (300 mg/kg/day i.p.) and antioxidative effects of β-glucan (4 mg/kg/day i.p.) and/or vitamin C (100 mg/kg/day i.p.) on the blood parameters of treated rats. After 3 days of treatment, hematological and parameters of redox status were measured. Exposure of rats to acetaminophen caused significant changes in some hematological parameters and the glutathione redox cycle, leading to an increased concentration of oxidative stress parameters and the formation of lipid peroxidation, while the activities of antioxidant enzymes were decreased. Administration of β-glucan and/or vitamin C reduced lipid peroxidation and restored the levels of examined hematological and oxidative stress parameters and improved the activities of antioxidant enzymes. Obtained results demonstrated that acetaminophen has significant pro-oxidative effects and may disrupt redox balance in blood of rats, while the combination of β-glucan and/or vitamin C amplified the antioxidant defense potential and exhibited a strong hematoprotective activity against acetaminophen-induced toxicity. Therefore, β-glucan and vitamin C co-treatment may be a promising therapeutic option for the treatment of acute acetaminophen hematotoxicity.
T2  - Drug and Chemical Toxicology
T1  - Hematoprotective effects and antioxidant properties of β-glucan and vitamin C against acetaminophen-induced toxicity: an experimental study in rats.
DO  - 10.1080/01480545.2019.1587451
ER  - 

@article{
author = "Matić, Miloš M. and Paunović, Milica G. and Milošević, Marija D. and Ognjanović, Branka I. and Saičić, Zorica",
year = "2019",
abstract = "Acetaminophen is widely used as an over-the-counter analgesic and antipyretic drug. The aim of the present study was to investigate the pro-oxidative effects of acetaminophen (300 mg/kg/day i.p.) and antioxidative effects of β-glucan (4 mg/kg/day i.p.) and/or vitamin C (100 mg/kg/day i.p.) on the blood parameters of treated rats. After 3 days of treatment, hematological and parameters of redox status were measured. Exposure of rats to acetaminophen caused significant changes in some hematological parameters and the glutathione redox cycle, leading to an increased concentration of oxidative stress parameters and the formation of lipid peroxidation, while the activities of antioxidant enzymes were decreased. Administration of β-glucan and/or vitamin C reduced lipid peroxidation and restored the levels of examined hematological and oxidative stress parameters and improved the activities of antioxidant enzymes. Obtained results demonstrated that acetaminophen has significant pro-oxidative effects and may disrupt redox balance in blood of rats, while the combination of β-glucan and/or vitamin C amplified the antioxidant defense potential and exhibited a strong hematoprotective activity against acetaminophen-induced toxicity. Therefore, β-glucan and vitamin C co-treatment may be a promising therapeutic option for the treatment of acute acetaminophen hematotoxicity.",
journal = "Drug and Chemical Toxicology",
title = "Hematoprotective effects and antioxidant properties of β-glucan and vitamin C against acetaminophen-induced toxicity: an experimental study in rats.",
doi = "10.1080/01480545.2019.1587451"
}

Matić, M. M., Paunović, M. G., Milošević, M. D., Ognjanović, B. I.,& Saičić, Z.. (2019). Hematoprotective effects and antioxidant properties of β-glucan and vitamin C against acetaminophen-induced toxicity: an experimental study in rats.. in Drug and Chemical Toxicology.
https://doi.org/10.1080/01480545.2019.1587451

Matić MM, Paunović MG, Milošević MD, Ognjanović BI, Saičić Z. Hematoprotective effects and antioxidant properties of β-glucan and vitamin C against acetaminophen-induced toxicity: an experimental study in rats.. in Drug and Chemical Toxicology. 2019;.
doi:10.1080/01480545.2019.1587451 .

Matić, Miloš M., Paunović, Milica G., Milošević, Marija D., Ognjanović, Branka I., Saičić, Zorica, "Hematoprotective effects and antioxidant properties of β-glucan and vitamin C against acetaminophen-induced toxicity: an experimental study in rats." in Drug and Chemical Toxicology (2019),
https://doi.org/10.1080/01480545.2019.1587451 . .

TY  - JOUR
AU  - Đurašević, Siniša
AU  - Jasnić, Nebojša
AU  - Prokić, Marko
AU  - Grigorov, Ilijana
AU  - Martinović, Vesna
AU  - Đorđević, Jelena
AU  - Pavlović, Slađan
PY  - 2019
UR  - http://xlink.rsc.org/?DOI=C9FO00107G
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3343
AB  - The aim of this study was to investigate the potential protective effect of virgin coconut oil (VCO) on oxidative stress parameters in the liver, kidneys and heart of alloxan-induced (150 mg kg-1 i.p.-1) diabetes in rats. Our results showed that daily supplementation of VCO (20% of food) for 16 weeks significantly (p < 0.05) ameliorates some deleterious effects caused by alloxan. VCO reduced the diabetes-related increase in food (82.15 ± 1.49 vs. 145.51 ± 4.81 g per kg b.m. per day) and water (305.49 ± 6.09 vs. 583.98 ± 14.80 mL per kg b.m. per day) intake, and the decrease in the body mass gain (0.56 ± 0.16 vs. -2.13 ± 0.49 g per 100 g b.m. per week). In all three tissues, diabetes caused an increase in the concentration of total glutathione and sulfhydryl groups, and catalase and glutathione S-transferase activities, without changes in superoxide dismutase activity. Glutathione peroxidase activity was increased in the kidneys and heart, but not in the liver of the diabetic animals, while glutathione reductase activity was increased in the liver and the kidneys, and not in the heart. The simultaneous VCO supplementation increased the concentration of the sulfhydryl group in all three tissues of diabetic animals and decreased the glutathione S-transferase activity and glutathione concentration, without affecting the glutathione reductase activity. In the liver of diabetic animals it decreased superoxide dismutase, catalase and glutathione peroxidase activities, in the heart catalase and glutathione peroxidase activities, and in the kidney catalase activity only. The results of canonical discriminant analysis of oxidative stress parameters revealed that VCO exerts its effects in a tissue-specific manner.
T2  - Food & Function
T1  - The protective role of virgin coconut oil on the alloxan-induced oxidative stress in the liver, kidneys and heart of diabetic rats.
IS  - 4
VL  - 10
DO  - 10.1039/c9fo00107g
SP  - 2114
EP  - 2124
ER  - 

@article{
author = "Đurašević, Siniša and Jasnić, Nebojša and Prokić, Marko and Grigorov, Ilijana and Martinović, Vesna and Đorđević, Jelena and Pavlović, Slađan",
year = "2019",
abstract = "The aim of this study was to investigate the potential protective effect of virgin coconut oil (VCO) on oxidative stress parameters in the liver, kidneys and heart of alloxan-induced (150 mg kg-1 i.p.-1) diabetes in rats. Our results showed that daily supplementation of VCO (20% of food) for 16 weeks significantly (p < 0.05) ameliorates some deleterious effects caused by alloxan. VCO reduced the diabetes-related increase in food (82.15 ± 1.49 vs. 145.51 ± 4.81 g per kg b.m. per day) and water (305.49 ± 6.09 vs. 583.98 ± 14.80 mL per kg b.m. per day) intake, and the decrease in the body mass gain (0.56 ± 0.16 vs. -2.13 ± 0.49 g per 100 g b.m. per week). In all three tissues, diabetes caused an increase in the concentration of total glutathione and sulfhydryl groups, and catalase and glutathione S-transferase activities, without changes in superoxide dismutase activity. Glutathione peroxidase activity was increased in the kidneys and heart, but not in the liver of the diabetic animals, while glutathione reductase activity was increased in the liver and the kidneys, and not in the heart. The simultaneous VCO supplementation increased the concentration of the sulfhydryl group in all three tissues of diabetic animals and decreased the glutathione S-transferase activity and glutathione concentration, without affecting the glutathione reductase activity. In the liver of diabetic animals it decreased superoxide dismutase, catalase and glutathione peroxidase activities, in the heart catalase and glutathione peroxidase activities, and in the kidney catalase activity only. The results of canonical discriminant analysis of oxidative stress parameters revealed that VCO exerts its effects in a tissue-specific manner.",
journal = "Food & Function",
title = "The protective role of virgin coconut oil on the alloxan-induced oxidative stress in the liver, kidneys and heart of diabetic rats.",
number = "4",
volume = "10",
doi = "10.1039/c9fo00107g",
pages = "2114-2124"
}

Đurašević, S., Jasnić, N., Prokić, M., Grigorov, I., Martinović, V., Đorđević, J.,& Pavlović, S.. (2019). The protective role of virgin coconut oil on the alloxan-induced oxidative stress in the liver, kidneys and heart of diabetic rats.. in Food & Function, 10(4), 2114-2124.
https://doi.org/10.1039/c9fo00107g

Đurašević S, Jasnić N, Prokić M, Grigorov I, Martinović V, Đorđević J, Pavlović S. The protective role of virgin coconut oil on the alloxan-induced oxidative stress in the liver, kidneys and heart of diabetic rats.. in Food & Function. 2019;10(4):2114-2124.
doi:10.1039/c9fo00107g .

Đurašević, Siniša, Jasnić, Nebojša, Prokić, Marko, Grigorov, Ilijana, Martinović, Vesna, Đorđević, Jelena, Pavlović, Slađan, "The protective role of virgin coconut oil on the alloxan-induced oxidative stress in the liver, kidneys and heart of diabetic rats." in Food & Function, 10, no. 4 (2019):2114-2124,
https://doi.org/10.1039/c9fo00107g . .

TY  - JOUR
AU  - Đurašević, Siniša
AU  - Jasnić, Nebojša
AU  - Prokić, Marko
AU  - Grigorov, Ilijana
AU  - Martinović, Vesna
AU  - Đorđević, Jelena
AU  - Pavlović, Slađan
PY  - 2019
UR  - http://xlink.rsc.org/?DOI=C9FO00107G
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3341
AB  - The aim of this study was to investigate the potential protective effect of virgin coconut oil (VCO) on oxidative stress parameters in the liver, kidneys and heart of alloxan-induced (150 mg kg-1 i.p.-1) diabetes in rats. Our results showed that daily supplementation of VCO (20% of food) for 16 weeks significantly (p < 0.05) ameliorates some deleterious effects caused by alloxan. VCO reduced the diabetes-related increase in food (82.15 ± 1.49 vs. 145.51 ± 4.81 g per kg b.m. per day) and water (305.49 ± 6.09 vs. 583.98 ± 14.80 mL per kg b.m. per day) intake, and the decrease in the body mass gain (0.56 ± 0.16 vs. -2.13 ± 0.49 g per 100 g b.m. per week). In all three tissues, diabetes caused an increase in the concentration of total glutathione and sulfhydryl groups, and catalase and glutathione S-transferase activities, without changes in superoxide dismutase activity. Glutathione peroxidase activity was increased in the kidneys and heart, but not in the liver of the diabetic animals, while glutathione reductase activity was increased in the liver and the kidneys, and not in the heart. The simultaneous VCO supplementation increased the concentration of the sulfhydryl group in all three tissues of diabetic animals and decreased the glutathione S-transferase activity and glutathione concentration, without affecting the glutathione reductase activity. In the liver of diabetic animals it decreased superoxide dismutase, catalase and glutathione peroxidase activities, in the heart catalase and glutathione peroxidase activities, and in the kidney catalase activity only. The results of canonical discriminant analysis of oxidative stress parameters revealed that VCO exerts its effects in a tissue-specific manner.
T2  - Food & Function
T1  - The protective role of virgin coconut oil on the alloxan-induced oxidative stress in the liver, kidneys and heart of diabetic rats.
IS  - 4
VL  - 10
DO  - 10.1039/c9fo00107g
SP  - 2114
EP  - 2124
ER  - 

@article{
author = "Đurašević, Siniša and Jasnić, Nebojša and Prokić, Marko and Grigorov, Ilijana and Martinović, Vesna and Đorđević, Jelena and Pavlović, Slađan",
year = "2019",
abstract = "The aim of this study was to investigate the potential protective effect of virgin coconut oil (VCO) on oxidative stress parameters in the liver, kidneys and heart of alloxan-induced (150 mg kg-1 i.p.-1) diabetes in rats. Our results showed that daily supplementation of VCO (20% of food) for 16 weeks significantly (p < 0.05) ameliorates some deleterious effects caused by alloxan. VCO reduced the diabetes-related increase in food (82.15 ± 1.49 vs. 145.51 ± 4.81 g per kg b.m. per day) and water (305.49 ± 6.09 vs. 583.98 ± 14.80 mL per kg b.m. per day) intake, and the decrease in the body mass gain (0.56 ± 0.16 vs. -2.13 ± 0.49 g per 100 g b.m. per week). In all three tissues, diabetes caused an increase in the concentration of total glutathione and sulfhydryl groups, and catalase and glutathione S-transferase activities, without changes in superoxide dismutase activity. Glutathione peroxidase activity was increased in the kidneys and heart, but not in the liver of the diabetic animals, while glutathione reductase activity was increased in the liver and the kidneys, and not in the heart. The simultaneous VCO supplementation increased the concentration of the sulfhydryl group in all three tissues of diabetic animals and decreased the glutathione S-transferase activity and glutathione concentration, without affecting the glutathione reductase activity. In the liver of diabetic animals it decreased superoxide dismutase, catalase and glutathione peroxidase activities, in the heart catalase and glutathione peroxidase activities, and in the kidney catalase activity only. The results of canonical discriminant analysis of oxidative stress parameters revealed that VCO exerts its effects in a tissue-specific manner.",
journal = "Food & Function",
title = "The protective role of virgin coconut oil on the alloxan-induced oxidative stress in the liver, kidneys and heart of diabetic rats.",
number = "4",
volume = "10",
doi = "10.1039/c9fo00107g",
pages = "2114-2124"
}

Đurašević, S., Jasnić, N., Prokić, M., Grigorov, I., Martinović, V., Đorđević, J.,& Pavlović, S.. (2019). The protective role of virgin coconut oil on the alloxan-induced oxidative stress in the liver, kidneys and heart of diabetic rats.. in Food & Function, 10(4), 2114-2124.
https://doi.org/10.1039/c9fo00107g

Đurašević S, Jasnić N, Prokić M, Grigorov I, Martinović V, Đorđević J, Pavlović S. The protective role of virgin coconut oil on the alloxan-induced oxidative stress in the liver, kidneys and heart of diabetic rats.. in Food & Function. 2019;10(4):2114-2124.
doi:10.1039/c9fo00107g .

Đurašević, Siniša, Jasnić, Nebojša, Prokić, Marko, Grigorov, Ilijana, Martinović, Vesna, Đorđević, Jelena, Pavlović, Slađan, "The protective role of virgin coconut oil on the alloxan-induced oxidative stress in the liver, kidneys and heart of diabetic rats." in Food & Function, 10, no. 4 (2019):2114-2124,
https://doi.org/10.1039/c9fo00107g . .

TY  - JOUR
AU  - Prokić, Marko
AU  - Gavrić, Jelena
AU  - Petrović, Tamara
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Krizmanić, Imre
AU  - Pavlović, Slađan
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S109564331930090X?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3338
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3409
AB  - During life, anuran individuals undergo drastic changes in the course of transition from aquatic to terrestrial habitat, when they are faced with metabolically demanding processes (growth, responses to developmental pressures), which result in increased production of reactive oxygen species (ROS), signaling molecules involved in development that can induce oxidative damage and stress. This situation can be further complicated by environmental influences. The aim of this study was to investigate oxidative stress parameters in naturally developing Pelophylax esculentus complex frogs during four developmental periods: premetamorphosis, prometamorphosis, metamorphic climax and juvenile stage, in order to examine changes in the response of the antioxidative system (AOS) and oxidative damage during the transition from aquatic to terrestrial life. Results show that ontogenetic shifts in anurans are accompanied by different levels of damage and AOS responses, which vary from the increased first-line enzymatic activities during the early period of development (premetamorphosis), through increased changes in the non-enzymatic complement during the metamorphic climax, to changes in both the enzymatic and non-enzymatic components observed in juvenile individuals. Premetamorphic individuals and individuals in metamorphosis displayed higher levels of lipid peroxidation, indicating that direct exposure to the environment for the first time and the modulation of organs are the most susceptible stages for oxidative damage. On the other hand, lower oxidative damage in juveniles points to the ability of their AOS to efficiently respond to challenges of the terrestrial environment. This study highlights the importance of ROS and the AOS of anurans in response to different developmental and/or environmental pressures that individuals face.
T2  - Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
T1  - Oxidative stress in Pelophylax esculentus complex frogs in the wild during transition from aquatic to terrestrial life
IS  - Part A
VL  - 234
DO  - 10.1016/J.CBPA.2019.05.004
SP  - 98
EP  - 105
ER  - 

@article{
author = "Prokić, Marko and Gavrić, Jelena and Petrović, Tamara and Despotović, Svetlana and Gavrilović, Branka and Radovanović, Tijana and Krizmanić, Imre and Pavlović, Slađan",
year = "2019",
abstract = "During life, anuran individuals undergo drastic changes in the course of transition from aquatic to terrestrial habitat, when they are faced with metabolically demanding processes (growth, responses to developmental pressures), which result in increased production of reactive oxygen species (ROS), signaling molecules involved in development that can induce oxidative damage and stress. This situation can be further complicated by environmental influences. The aim of this study was to investigate oxidative stress parameters in naturally developing Pelophylax esculentus complex frogs during four developmental periods: premetamorphosis, prometamorphosis, metamorphic climax and juvenile stage, in order to examine changes in the response of the antioxidative system (AOS) and oxidative damage during the transition from aquatic to terrestrial life. Results show that ontogenetic shifts in anurans are accompanied by different levels of damage and AOS responses, which vary from the increased first-line enzymatic activities during the early period of development (premetamorphosis), through increased changes in the non-enzymatic complement during the metamorphic climax, to changes in both the enzymatic and non-enzymatic components observed in juvenile individuals. Premetamorphic individuals and individuals in metamorphosis displayed higher levels of lipid peroxidation, indicating that direct exposure to the environment for the first time and the modulation of organs are the most susceptible stages for oxidative damage. On the other hand, lower oxidative damage in juveniles points to the ability of their AOS to efficiently respond to challenges of the terrestrial environment. This study highlights the importance of ROS and the AOS of anurans in response to different developmental and/or environmental pressures that individuals face.",
journal = "Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology",
title = "Oxidative stress in Pelophylax esculentus complex frogs in the wild during transition from aquatic to terrestrial life",
number = "Part A",
volume = "234",
doi = "10.1016/J.CBPA.2019.05.004",
pages = "98-105"
}

Prokić, M., Gavrić, J., Petrović, T., Despotović, S., Gavrilović, B., Radovanović, T., Krizmanić, I.,& Pavlović, S.. (2019). Oxidative stress in Pelophylax esculentus complex frogs in the wild during transition from aquatic to terrestrial life. in Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 234(Part A), 98-105.
https://doi.org/10.1016/J.CBPA.2019.05.004

Prokić M, Gavrić J, Petrović T, Despotović S, Gavrilović B, Radovanović T, Krizmanić I, Pavlović S. Oxidative stress in Pelophylax esculentus complex frogs in the wild during transition from aquatic to terrestrial life. in Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. 2019;234(Part A):98-105.
doi:10.1016/J.CBPA.2019.05.004 .

Prokić, Marko, Gavrić, Jelena, Petrović, Tamara, Despotović, Svetlana, Gavrilović, Branka, Radovanović, Tijana, Krizmanić, Imre, Pavlović, Slađan, "Oxidative stress in Pelophylax esculentus complex frogs in the wild during transition from aquatic to terrestrial life" in Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 234, no. Part A (2019):98-105,
https://doi.org/10.1016/J.CBPA.2019.05.004 . .

TY  - JOUR
AU  - Vujotić, Ljiljana
AU  - Matić, Siniša
AU  - Borković Mitić, Slavica
AU  - Stojsavljević, Aleksandar
AU  - Mutić, Jelena
AU  - Baščarević, Vladimir
AU  - Joković, Miloš
AU  - Pavlović, Slađan
PY  - 2019
UR  - https://www.termedia.pl/Association-between-oxidative-stress-biomarkers-and-concentrations-of-some-metal-ions-in-the-blood-of-patients-with-brain-tumors-and-hydrocephalus,19,37458,1,1.html
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3459
AB  - Introduction
Any substance that induces production of free radicals can be a potential cause of brain damage. The aim of our study was to investigate the relationship between some metal ions and oxidative stress biomarkers in the blood of patients with brain tumor and hydrocephalus.

Material and methods
Our study included 27 control subjects, 24 patients with brain tumor and 21 patients with hydrocephalus. The activities of superoxide dismutase (CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), glutathione S-transferase (GST) and acetyl cholinesterase (AChE), as well as concentrations of reduced glutathione (GSH), lipid peroxides (TBARS) and sulfhydryl groups (SH) were analyzed in the plasma and red blood cells (RBCs) of patients. We also determined the concentrations of Mn, Ni, Co, Cu, Zn, As, Se, Cd, Hg and Fe.

Results
The higher activity of SOD and concentration of GSH in both investigated groups could indicate higher oxidative stress. We also observed decreased levels of SH groups in both groups of patients. In both groups of patients we detected decreased concentrations of Ni, Co, Zn and Fe (and Mn in brain tumor patients) and increased concentrations of As, Se and Cd in the blood. Interestingly, we observed a higher concentration of Cd in both plasma and RBCs of hydrocephalus patients compared to the patients with brain tumor.

Conclusions
There are strong correlations between some metal ion concentrations and certain oxidative stress biomarkers in the blood of patients, which supports our hypothesis, but the observed trend needs to be further investigated.
PB  - Termedia & Banach
T2  - Archives of Medical Science
T1  - Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus
DO  - 10.5114/aoms.2019.87409
ER  - 

@article{
author = "Vujotić, Ljiljana and Matić, Siniša and Borković Mitić, Slavica and Stojsavljević, Aleksandar and Mutić, Jelena and Baščarević, Vladimir and Joković, Miloš and Pavlović, Slađan",
year = "2019",
abstract = "Introduction
Any substance that induces production of free radicals can be a potential cause of brain damage. The aim of our study was to investigate the relationship between some metal ions and oxidative stress biomarkers in the blood of patients with brain tumor and hydrocephalus.

Material and methods
Our study included 27 control subjects, 24 patients with brain tumor and 21 patients with hydrocephalus. The activities of superoxide dismutase (CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), glutathione S-transferase (GST) and acetyl cholinesterase (AChE), as well as concentrations of reduced glutathione (GSH), lipid peroxides (TBARS) and sulfhydryl groups (SH) were analyzed in the plasma and red blood cells (RBCs) of patients. We also determined the concentrations of Mn, Ni, Co, Cu, Zn, As, Se, Cd, Hg and Fe.

Results
The higher activity of SOD and concentration of GSH in both investigated groups could indicate higher oxidative stress. We also observed decreased levels of SH groups in both groups of patients. In both groups of patients we detected decreased concentrations of Ni, Co, Zn and Fe (and Mn in brain tumor patients) and increased concentrations of As, Se and Cd in the blood. Interestingly, we observed a higher concentration of Cd in both plasma and RBCs of hydrocephalus patients compared to the patients with brain tumor.

Conclusions
There are strong correlations between some metal ion concentrations and certain oxidative stress biomarkers in the blood of patients, which supports our hypothesis, but the observed trend needs to be further investigated.",
publisher = "Termedia & Banach",
journal = "Archives of Medical Science",
title = "Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus",
doi = "10.5114/aoms.2019.87409"
}

Vujotić, L., Matić, S., Borković Mitić, S., Stojsavljević, A., Mutić, J., Baščarević, V., Joković, M.,& Pavlović, S.. (2019). Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus. in Archives of Medical Science
Termedia & Banach..
https://doi.org/10.5114/aoms.2019.87409

Vujotić L, Matić S, Borković Mitić S, Stojsavljević A, Mutić J, Baščarević V, Joković M, Pavlović S. Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus. in Archives of Medical Science. 2019;.
doi:10.5114/aoms.2019.87409 .

Vujotić, Ljiljana, Matić, Siniša, Borković Mitić, Slavica, Stojsavljević, Aleksandar, Mutić, Jelena, Baščarević, Vladimir, Joković, Miloš, Pavlović, Slađan, "Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus" in Archives of Medical Science (2019),
https://doi.org/10.5114/aoms.2019.87409 . .

TY  - JOUR
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Prokić, Marko
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Anđelković, Marko
AU  - Tomović, Ljiljana
AU  - Borković Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Saičić, Zorica
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S1532045619300924?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3351
AB  - In this study we examined possible differences in heavy metal accumulation and oxidative stress parameters in the liver and muscle of two semi-aquatic snakes: grass snake (Natrix natrix) and dice snake (N. tessellata), that inhabit the same environment but differ in prey diversity. The obtained results revealed some interspecies, inter-tissue, prey-snake and prey-prey differences in heavy metal concentrations. Grass snakes pray contained significantly higher concentrations of Al, Cr and Fe as compared to food of dice snakes. Both investigated snakes accumulated generally lower concentrations of metals than their prey, indicating that they are not at risk of contaminant biomagnification. A significant interspecies difference in accumulation was observed only for Cu and Mn concentrations. On the other hand, analysis of oxidative stress biomarkers showed clear differences between the investigated snake species and the two investigated tissues. The liver of grass snake had increased superoxide dismutase, glutathione reductase and glutathione-S-transferase activities in comparison to dice snake. In muscle, a reverse trend was observed for the activities of these three enzymes, as well as for glutathione peroxidase activity. The higher number of significant correlations observed between oxidative stress biomarkers and heavy metal concentrations in grass snake points to upregulation of the antioxidative system (AOS), which resulted in a lower TBARS concentration. Results show that while the investigated snake species did not differ significantly in the accumulated metals, their defense mechanisms were different. This reveals the complexity of the AOS and points to the cooperation of different AOS components in individuals from natural populations.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?
DO  - 10.1016/J.CBPC.2019.05.010
ER  - 

@article{
author = "Gavrić, Jelena and Despotović, Svetlana and Prokić, Marko and Gavrilović, Branka and Radovanović, Tijana and Anđelković, Marko and Tomović, Ljiljana and Borković Mitić, Slavica and Pavlović, Slađan and Saičić, Zorica",
year = "2019",
abstract = "In this study we examined possible differences in heavy metal accumulation and oxidative stress parameters in the liver and muscle of two semi-aquatic snakes: grass snake (Natrix natrix) and dice snake (N. tessellata), that inhabit the same environment but differ in prey diversity. The obtained results revealed some interspecies, inter-tissue, prey-snake and prey-prey differences in heavy metal concentrations. Grass snakes pray contained significantly higher concentrations of Al, Cr and Fe as compared to food of dice snakes. Both investigated snakes accumulated generally lower concentrations of metals than their prey, indicating that they are not at risk of contaminant biomagnification. A significant interspecies difference in accumulation was observed only for Cu and Mn concentrations. On the other hand, analysis of oxidative stress biomarkers showed clear differences between the investigated snake species and the two investigated tissues. The liver of grass snake had increased superoxide dismutase, glutathione reductase and glutathione-S-transferase activities in comparison to dice snake. In muscle, a reverse trend was observed for the activities of these three enzymes, as well as for glutathione peroxidase activity. The higher number of significant correlations observed between oxidative stress biomarkers and heavy metal concentrations in grass snake points to upregulation of the antioxidative system (AOS), which resulted in a lower TBARS concentration. Results show that while the investigated snake species did not differ significantly in the accumulated metals, their defense mechanisms were different. This reveals the complexity of the AOS and points to the cooperation of different AOS components in individuals from natural populations.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?",
doi = "10.1016/J.CBPC.2019.05.010"
}

Gavrić, J., Despotović, S., Prokić, M., Gavrilović, B., Radovanović, T., Anđelković, M., Tomović, L., Borković Mitić, S., Pavlović, S.,& Saičić, Z.. (2019). Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology.
https://doi.org/10.1016/J.CBPC.2019.05.010

Gavrić J, Despotović S, Prokić M, Gavrilović B, Radovanović T, Anđelković M, Tomović L, Borković Mitić S, Pavlović S, Saičić Z. Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2019;.
doi:10.1016/J.CBPC.2019.05.010 .

Gavrić, Jelena, Despotović, Svetlana, Prokić, Marko, Gavrilović, Branka, Radovanović, Tijana, Anđelković, Marko, Tomović, Ljiljana, Borković Mitić, Slavica, Pavlović, Slađan, Saičić, Zorica, "Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?" in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology (2019),
https://doi.org/10.1016/J.CBPC.2019.05.010 . .

TY  - JOUR
AU  - Despotović, Svetlana
AU  - Prokić, Marko
AU  - Gavrić, Jelena
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Borković Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Saičić, Zorica
PY  - 2019
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641800045D
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/3270
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3355
AB  - Metal pollution of the aquatic environment is of global concern because metals are ubiquitous and can be accumulated in natural habitats as well as in organisms through the food chain. Accumulated metals are capable of inducing toxicity in living organisms, altering their reproductive success, behavior, immune response and biochemical processes. We examined the correlation between the concentrations of 9 metals (As, Cd, Cr, Cu, Fe, Mn, Ni, Pb and Zn) in the whole body of the river snail Viviparus acerosus, river water and sediment from three Serbian rivers with different levels of metal pollution, the Danube, Tisa and Velika Morava. Data about water quality showed that the concentrations of As, Cr, Fe and Ni were highest in the water of the Danube and of Cu, Mn and Zn in the water of the Velika Morava River. The concentrations of As and Mn were highest in the Danube River sediment, of Cd, Cu, Fe, Pb and Zn in the Tisa and of Cr and Ni in the sediment of the Velika Morava. The concentrations of all of the examined metals, except for Cu, were highest in snails from the Velika Morava. Correspondence analysis showed stronger correlations between metal concentrations in snails and the river sediment than between snails and river water. Several correlations between metal concentrations in snails and river sediment and water were established by Pearson’s correlation test. The concentrations of metals in snail bodies were affected to a greater extent by the river sediment than by the river-water metal content. We conclude that V. acerosus has great potential as a bioindicator species of metal pollution in freshwater basins.
T2  - Archives of Biological Sciences
T1  - Evaluation of the river snail Viviparus acerosus as a potential bioindicator species of metal pollution in freshwater ecosystems
IS  - 1
VL  - 71
DO  - 10.2298/ABS180801045D
SP  - 39
EP  - 47
ER  - 

@article{
author = "Despotović, Svetlana and Prokić, Marko and Gavrić, Jelena and Gavrilović, Branka and Radovanović, Tijana and Borković Mitić, Slavica and Pavlović, Slađan and Saičić, Zorica",
year = "2019",
abstract = "Metal pollution of the aquatic environment is of global concern because metals are ubiquitous and can be accumulated in natural habitats as well as in organisms through the food chain. Accumulated metals are capable of inducing toxicity in living organisms, altering their reproductive success, behavior, immune response and biochemical processes. We examined the correlation between the concentrations of 9 metals (As, Cd, Cr, Cu, Fe, Mn, Ni, Pb and Zn) in the whole body of the river snail Viviparus acerosus, river water and sediment from three Serbian rivers with different levels of metal pollution, the Danube, Tisa and Velika Morava. Data about water quality showed that the concentrations of As, Cr, Fe and Ni were highest in the water of the Danube and of Cu, Mn and Zn in the water of the Velika Morava River. The concentrations of As and Mn were highest in the Danube River sediment, of Cd, Cu, Fe, Pb and Zn in the Tisa and of Cr and Ni in the sediment of the Velika Morava. The concentrations of all of the examined metals, except for Cu, were highest in snails from the Velika Morava. Correspondence analysis showed stronger correlations between metal concentrations in snails and the river sediment than between snails and river water. Several correlations between metal concentrations in snails and river sediment and water were established by Pearson’s correlation test. The concentrations of metals in snail bodies were affected to a greater extent by the river sediment than by the river-water metal content. We conclude that V. acerosus has great potential as a bioindicator species of metal pollution in freshwater basins.",
journal = "Archives of Biological Sciences",
title = "Evaluation of the river snail Viviparus acerosus as a potential bioindicator species of metal pollution in freshwater ecosystems",
number = "1",
volume = "71",
doi = "10.2298/ABS180801045D",
pages = "39-47"
}

Despotović, S., Prokić, M., Gavrić, J., Gavrilović, B., Radovanović, T., Borković Mitić, S., Pavlović, S.,& Saičić, Z.. (2019). Evaluation of the river snail Viviparus acerosus as a potential bioindicator species of metal pollution in freshwater ecosystems. in Archives of Biological Sciences, 71(1), 39-47.
https://doi.org/10.2298/ABS180801045D

Despotović S, Prokić M, Gavrić J, Gavrilović B, Radovanović T, Borković Mitić S, Pavlović S, Saičić Z. Evaluation of the river snail Viviparus acerosus as a potential bioindicator species of metal pollution in freshwater ecosystems. in Archives of Biological Sciences. 2019;71(1):39-47.
doi:10.2298/ABS180801045D .

Despotović, Svetlana, Prokić, Marko, Gavrić, Jelena, Gavrilović, Branka, Radovanović, Tijana, Borković Mitić, Slavica, Pavlović, Slađan, Saičić, Zorica, "Evaluation of the river snail Viviparus acerosus as a potential bioindicator species of metal pollution in freshwater ecosystems" in Archives of Biological Sciences, 71, no. 1 (2019):39-47,
https://doi.org/10.2298/ABS180801045D . .

TY  - JOUR
AU  - Prokić, Marko
AU  - Radovanović, Tijana
AU  - Gavrić, Jelena
AU  - Faggio, Caterina
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0165993618304710?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3222
AB  - Interest about interactions between microplastics and organisms is on the rise. Accessing organisms' responses to these chemically “inert” compounds plays an important role in determining their potential toxicity. Microplastics from the environment tend to accumulate and move through living organisms, inducing a variety of biological effects, such as disturbances in energy metabolism, oxidative balance, antioxidative capacity, DNA, immunological, neurological and histological damage. In the present review, we summarized the adverse effects of different size, concentrations and types of microplastics on animals' antioxidative system, energy metabolism and nervous system. Results showed that microplastics can: induce oxidative damage (increased lipid peroxidation and DNA strand breaks); alter antioxidative system (superoxide dismutase, catalase and glutathione peroxidase were parameters with the highest and significant changes in activities) and metabolism (isocitrate dehydrogenase and lactate dehydrogenase activity); and have neurotoxic effects (inhibition of acetylcholinesterase activity). The effects were depending on size and dose of used microplastics, and/or their interaction with other xenobiotics. We examined also potential strategies and offer research priorities for current and future studies.
T2  - TrAC Trends in Analytical Chemistry
T1  - Ecotoxicological effects of microplastics: Examination of biomarkers, current state and future perspectives
VL  - 111
DO  - 10.1016/J.TRAC.2018.12.001
SP  - 37
EP  - 46
ER  - 

@article{
author = "Prokić, Marko and Radovanović, Tijana and Gavrić, Jelena and Faggio, Caterina",
year = "2019",
abstract = "Interest about interactions between microplastics and organisms is on the rise. Accessing organisms' responses to these chemically “inert” compounds plays an important role in determining their potential toxicity. Microplastics from the environment tend to accumulate and move through living organisms, inducing a variety of biological effects, such as disturbances in energy metabolism, oxidative balance, antioxidative capacity, DNA, immunological, neurological and histological damage. In the present review, we summarized the adverse effects of different size, concentrations and types of microplastics on animals' antioxidative system, energy metabolism and nervous system. Results showed that microplastics can: induce oxidative damage (increased lipid peroxidation and DNA strand breaks); alter antioxidative system (superoxide dismutase, catalase and glutathione peroxidase were parameters with the highest and significant changes in activities) and metabolism (isocitrate dehydrogenase and lactate dehydrogenase activity); and have neurotoxic effects (inhibition of acetylcholinesterase activity). The effects were depending on size and dose of used microplastics, and/or their interaction with other xenobiotics. We examined also potential strategies and offer research priorities for current and future studies.",
journal = "TrAC Trends in Analytical Chemistry",
title = "Ecotoxicological effects of microplastics: Examination of biomarkers, current state and future perspectives",
volume = "111",
doi = "10.1016/J.TRAC.2018.12.001",
pages = "37-46"
}

Prokić, M., Radovanović, T., Gavrić, J.,& Faggio, C.. (2019). Ecotoxicological effects of microplastics: Examination of biomarkers, current state and future perspectives. in TrAC Trends in Analytical Chemistry, 111, 37-46.
https://doi.org/10.1016/J.TRAC.2018.12.001

Prokić M, Radovanović T, Gavrić J, Faggio C. Ecotoxicological effects of microplastics: Examination of biomarkers, current state and future perspectives. in TrAC Trends in Analytical Chemistry. 2019;111:37-46.
doi:10.1016/J.TRAC.2018.12.001 .

Prokić, Marko, Radovanović, Tijana, Gavrić, Jelena, Faggio, Caterina, "Ecotoxicological effects of microplastics: Examination of biomarkers, current state and future perspectives" in TrAC Trends in Analytical Chemistry, 111 (2019):37-46,
https://doi.org/10.1016/J.TRAC.2018.12.001 . .

TY  - JOUR
AU  - Prokić, Marko
AU  - Gavrić, Jelena
AU  - Petrović, Tamara
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Krizmanić, Imre
AU  - Pavlović, Slađan
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S109564331930090X?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3338
AB  - During life, anuran individuals undergo drastic changes in the course of transition from aquatic to terrestrial habitat, when they are faced with metabolically demanding processes (growth, responses to developmental pressures), which result in increased production of reactive oxygen species (ROS), signaling molecules involved in development that can induce oxidative damage and stress. This situation can be further complicated by environmental influences. The aim of this study was to investigate oxidative stress parameters in naturally developing Pelophylax esculentus complex frogs during four developmental periods: premetamorphosis, prometamorphosis, metamorphic climax and juvenile stage, in order to examine changes in the response of the antioxidative system (AOS) and oxidative damage during the transition from aquatic to terrestrial life. Results show that ontogenetic shifts in anurans are accompanied by different levels of damage and AOS responses, which vary from the increased first-line enzymatic activities during the early period of development (premetamorphosis), through increased changes in the non-enzymatic complement during the metamorphic climax, to changes in both the enzymatic and non-enzymatic components observed in juvenile individuals. Premetamorphic individuals and individuals in metamorphosis displayed higher levels of lipid peroxidation, indicating that direct exposure to the environment for the first time and the modulation of organs are the most susceptible stages for oxidative damage. On the other hand, lower oxidative damage in juveniles points to the ability of their AOS to efficiently respond to challenges of the terrestrial environment. This study highlights the importance of ROS and the AOS of anurans in response to different developmental and/or environmental pressures that individuals face.
T2  - Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
T1  - Oxidative stress in Pelophylax esculentus complex frogs in the wild during transition from aquatic to terrestrial life
DO  - 10.1016/J.CBPA.2019.05.004
ER  - 

@article{
author = "Prokić, Marko and Gavrić, Jelena and Petrović, Tamara and Despotović, Svetlana and Gavrilović, Branka and Radovanović, Tijana and Krizmanić, Imre and Pavlović, Slađan",
year = "2019",
abstract = "During life, anuran individuals undergo drastic changes in the course of transition from aquatic to terrestrial habitat, when they are faced with metabolically demanding processes (growth, responses to developmental pressures), which result in increased production of reactive oxygen species (ROS), signaling molecules involved in development that can induce oxidative damage and stress. This situation can be further complicated by environmental influences. The aim of this study was to investigate oxidative stress parameters in naturally developing Pelophylax esculentus complex frogs during four developmental periods: premetamorphosis, prometamorphosis, metamorphic climax and juvenile stage, in order to examine changes in the response of the antioxidative system (AOS) and oxidative damage during the transition from aquatic to terrestrial life. Results show that ontogenetic shifts in anurans are accompanied by different levels of damage and AOS responses, which vary from the increased first-line enzymatic activities during the early period of development (premetamorphosis), through increased changes in the non-enzymatic complement during the metamorphic climax, to changes in both the enzymatic and non-enzymatic components observed in juvenile individuals. Premetamorphic individuals and individuals in metamorphosis displayed higher levels of lipid peroxidation, indicating that direct exposure to the environment for the first time and the modulation of organs are the most susceptible stages for oxidative damage. On the other hand, lower oxidative damage in juveniles points to the ability of their AOS to efficiently respond to challenges of the terrestrial environment. This study highlights the importance of ROS and the AOS of anurans in response to different developmental and/or environmental pressures that individuals face.",
journal = "Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology",
title = "Oxidative stress in Pelophylax esculentus complex frogs in the wild during transition from aquatic to terrestrial life",
doi = "10.1016/J.CBPA.2019.05.004"
}

Prokić, M., Gavrić, J., Petrović, T., Despotović, S., Gavrilović, B., Radovanović, T., Krizmanić, I.,& Pavlović, S.. (2019). Oxidative stress in Pelophylax esculentus complex frogs in the wild during transition from aquatic to terrestrial life. in Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology.
https://doi.org/10.1016/J.CBPA.2019.05.004

Prokić M, Gavrić J, Petrović T, Despotović S, Gavrilović B, Radovanović T, Krizmanić I, Pavlović S. Oxidative stress in Pelophylax esculentus complex frogs in the wild during transition from aquatic to terrestrial life. in Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. 2019;.
doi:10.1016/J.CBPA.2019.05.004 .

Prokić, Marko, Gavrić, Jelena, Petrović, Tamara, Despotović, Svetlana, Gavrilović, Branka, Radovanović, Tijana, Krizmanić, Imre, Pavlović, Slađan, "Oxidative stress in Pelophylax esculentus complex frogs in the wild during transition from aquatic to terrestrial life" in Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology (2019),
https://doi.org/10.1016/J.CBPA.2019.05.004 . .

TY  - JOUR
AU  - Pavlović, Slađan
AU  - Borković Mitić, Slavica
AU  - Radovanović, Tijana
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Prokić, Marko
AU  - Saičić, Zorica
PY  - 2018
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641700059P
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3019
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/2500
AB  - We investigated the integrated response of antioxidant defense enzymes (total superoxide dismutase (TotSOD), manganese-containing superoxide dismutase (MnSOD), copper-zinc-containing superoxide dismutase (CuZnSOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and phase II biotransformation enzyme, glutathione- S-transferase (GST)) in the liver and white muscle of females of European hake (Merluccius merluccius L.) from the Adriatic Sea (Montenegro) in winter and spring. The activity of GSH-Px in the liver was significantly increased, while GST activity was decreased in spring compared to the winter. In white muscle, the activities of TotSOD and CuZnSOD were increased, while the activities of MnSOD, CAT, GSH-Px, GR and GST were decreased in spring when compared to the matching values in winter. The activities of TotSOD and CuZnSOD in winter were markedly lower in the muscle than in the liver, while the activity of MnSOD in the muscle was higher when compared to the liver. Principal component analysis (PCA) revealed clear separation of the investigated antioxidant biomarkers between tissues and seasons, while the integrated biomarker response (IBR) showed that the most intensive antioxidant biomarker response was in the liver in spring. Star plots of IBR showed a dominant contribution of glutathione-dependent biomarkers (GSH-Px, GR and GST) and CAT in both tissues and seasons with respect to SOD isoenzymes. All enzyme activities (except MnSOD) were greater in the liver in comparison to the white muscle. Our results show that the liver possesses a greater capacity to establish and maintain homeostasis under changing environmental conditions in winter and spring. At the same time, seasonal effects are more pronounced in muscle tissue
T2  - Archives of Biological Sciences
T1  - Integrated response of antioxidant biomarkers in the liver and white muscle of European hake (Merluccius merluccius L.) females from the Adriatic sea with respect to environmental influences
IS  - 1
VL  - 70
DO  - 10.2298/ABS171009059P
SP  - 205
EP  - 214
ER  - 

@article{
author = "Pavlović, Slađan and Borković Mitić, Slavica and Radovanović, Tijana and Gavrilović, Branka and Despotović, Svetlana and Gavrić, Jelena and Prokić, Marko and Saičić, Zorica",
year = "2018",
abstract = "We investigated the integrated response of antioxidant defense enzymes (total superoxide dismutase (TotSOD), manganese-containing superoxide dismutase (MnSOD), copper-zinc-containing superoxide dismutase (CuZnSOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and phase II biotransformation enzyme, glutathione- S-transferase (GST)) in the liver and white muscle of females of European hake (Merluccius merluccius L.) from the Adriatic Sea (Montenegro) in winter and spring. The activity of GSH-Px in the liver was significantly increased, while GST activity was decreased in spring compared to the winter. In white muscle, the activities of TotSOD and CuZnSOD were increased, while the activities of MnSOD, CAT, GSH-Px, GR and GST were decreased in spring when compared to the matching values in winter. The activities of TotSOD and CuZnSOD in winter were markedly lower in the muscle than in the liver, while the activity of MnSOD in the muscle was higher when compared to the liver. Principal component analysis (PCA) revealed clear separation of the investigated antioxidant biomarkers between tissues and seasons, while the integrated biomarker response (IBR) showed that the most intensive antioxidant biomarker response was in the liver in spring. Star plots of IBR showed a dominant contribution of glutathione-dependent biomarkers (GSH-Px, GR and GST) and CAT in both tissues and seasons with respect to SOD isoenzymes. All enzyme activities (except MnSOD) were greater in the liver in comparison to the white muscle. Our results show that the liver possesses a greater capacity to establish and maintain homeostasis under changing environmental conditions in winter and spring. At the same time, seasonal effects are more pronounced in muscle tissue",
journal = "Archives of Biological Sciences",
title = "Integrated response of antioxidant biomarkers in the liver and white muscle of European hake (Merluccius merluccius L.) females from the Adriatic sea with respect to environmental influences",
number = "1",
volume = "70",
doi = "10.2298/ABS171009059P",
pages = "205-214"
}

Pavlović, S., Borković Mitić, S., Radovanović, T., Gavrilović, B., Despotović, S., Gavrić, J., Prokić, M.,& Saičić, Z.. (2018). Integrated response of antioxidant biomarkers in the liver and white muscle of European hake (Merluccius merluccius L.) females from the Adriatic sea with respect to environmental influences. in Archives of Biological Sciences, 70(1), 205-214.
https://doi.org/10.2298/ABS171009059P

Pavlović S, Borković Mitić S, Radovanović T, Gavrilović B, Despotović S, Gavrić J, Prokić M, Saičić Z. Integrated response of antioxidant biomarkers in the liver and white muscle of European hake (Merluccius merluccius L.) females from the Adriatic sea with respect to environmental influences. in Archives of Biological Sciences. 2018;70(1):205-214.
doi:10.2298/ABS171009059P .

Pavlović, Slađan, Borković Mitić, Slavica, Radovanović, Tijana, Gavrilović, Branka, Despotović, Svetlana, Gavrić, Jelena, Prokić, Marko, Saičić, Zorica, "Integrated response of antioxidant biomarkers in the liver and white muscle of European hake (Merluccius merluccius L.) females from the Adriatic sea with respect to environmental influences" in Archives of Biological Sciences, 70, no. 1 (2018):205-214,
https://doi.org/10.2298/ABS171009059P . .

TY  - DATA
AU  - Pavlović, Slađan
AU  - Borković Mitić, Slavica
AU  - Radovanović, Tijana
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Prokić, Marko
AU  - Saičić, Zorica
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3716
AB  - Fig. S1. The geographical position of the investigated locality (Platamuni) in the Adriatic Sea.
T2  - Archives of Biological Sciences
T1  - Supplementary data for the article: Pavlović S, Borković-Mitić S, Radovanović T, Gavrilović B, Despotović S, Gavrić J, Prokić M, Saičić Z. Integrated response of antioxidant biomarkers in the liver and white muscle of European hake (Merluccius merluccius L.) females from the Adriatic sea with respect to environmental influences. Arch Biol Sci. 2018;70(1):205–14.
IS  - 1
VL  - 70
SP  - 205
EP  - 214
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3716
ER  - 

@misc{
author = "Pavlović, Slađan and Borković Mitić, Slavica and Radovanović, Tijana and Gavrilović, Branka and Despotović, Svetlana and Gavrić, Jelena and Prokić, Marko and Saičić, Zorica",
year = "2018",
abstract = "Fig. S1. The geographical position of the investigated locality (Platamuni) in the Adriatic Sea.",
journal = "Archives of Biological Sciences",
title = "Supplementary data for the article: Pavlović S, Borković-Mitić S, Radovanović T, Gavrilović B, Despotović S, Gavrić J, Prokić M, Saičić Z. Integrated response of antioxidant biomarkers in the liver and white muscle of European hake (Merluccius merluccius L.) females from the Adriatic sea with respect to environmental influences. Arch Biol Sci. 2018;70(1):205–14.",
number = "1",
volume = "70",
pages = "205-214",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3716"
}

Pavlović, S., Borković Mitić, S., Radovanović, T., Gavrilović, B., Despotović, S., Gavrić, J., Prokić, M.,& Saičić, Z.. (2018). Supplementary data for the article: Pavlović S, Borković-Mitić S, Radovanović T, Gavrilović B, Despotović S, Gavrić J, Prokić M, Saičić Z. Integrated response of antioxidant biomarkers in the liver and white muscle of European hake (Merluccius merluccius L.) females from the Adriatic sea with respect to environmental influences. Arch Biol Sci. 2018;70(1):205–14.. in Archives of Biological Sciences, 70(1), 205-214.
https://hdl.handle.net/21.15107/rcub_ibiss_3716

Pavlović S, Borković Mitić S, Radovanović T, Gavrilović B, Despotović S, Gavrić J, Prokić M, Saičić Z. Supplementary data for the article: Pavlović S, Borković-Mitić S, Radovanović T, Gavrilović B, Despotović S, Gavrić J, Prokić M, Saičić Z. Integrated response of antioxidant biomarkers in the liver and white muscle of European hake (Merluccius merluccius L.) females from the Adriatic sea with respect to environmental influences. Arch Biol Sci. 2018;70(1):205–14.. in Archives of Biological Sciences. 2018;70(1):205-214.
https://hdl.handle.net/21.15107/rcub_ibiss_3716 .

Pavlović, Slađan, Borković Mitić, Slavica, Radovanović, Tijana, Gavrilović, Branka, Despotović, Svetlana, Gavrić, Jelena, Prokić, Marko, Saičić, Zorica, "Supplementary data for the article: Pavlović S, Borković-Mitić S, Radovanović T, Gavrilović B, Despotović S, Gavrić J, Prokić M, Saičić Z. Integrated response of antioxidant biomarkers in the liver and white muscle of European hake (Merluccius merluccius L.) females from the Adriatic sea with respect to environmental influences. Arch Biol Sci. 2018;70(1):205–14." in Archives of Biological Sciences, 70, no. 1 (2018):205-214,
https://hdl.handle.net/21.15107/rcub_ibiss_3716 .

TY  - JOUR
AU  - Prokić, Marko
AU  - Petrović, Tamara
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Faggio, Caterina
AU  - Saičić, Zorica
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0944200618300242?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3144
AB  - During their complex life cycle, anurans are faced with various physiological and biochemical demands that can result in increased free radical production. The antioxidative system (AOS), assumes a central role in protection from oxidative stress, and increased knowledge of its response would allow us to identify and quantify underlying costs of free radical production. In this study, we compared the patterns and levels of integration of the AOS during two life stages of Bufotes viridis toads from natural populations: young, fully developed, reproductively inactive subadults and reproductively active adults. All examined parameters (SOD, CAT, GSH-Px, GR, GST, GSH and SH groups) were measured in the liver, skin and muscle. The obtained results showed that: (1) subadult individuals possessed a more integrated AOS (a higher index of integration and number of significant correlations), based mostly on the coordinated action of SOD-CAT; (2) adult individuals used the GSH-Px/GSH system; (3) all examined tissues exhibited similar patterns of variation of AOS parameters; (4) significant correlations between the AOS and biometric parameters. Our results indicate that adult and subadult toads differed in the way they processed free radicals, implying that adults may be more susceptible to oxidative damage. The knowledge from this study will contribute to a better understanding of the AOS and how its activity is modulated in natural populations of anurans throughout their life.
T2  - Zoology
T1  - Comparative assessment of the antioxidative defense system in subadult and adult anurans: A lesson from the Bufotes viridis toad
VL  - 130
DO  - 10.1016/J.ZOOL.2018.08.001
SP  - 30
EP  - 37
ER  - 

@article{
author = "Prokić, Marko and Petrović, Tamara and Gavrić, Jelena and Despotović, Svetlana and Gavrilović, Branka and Radovanović, Tijana and Faggio, Caterina and Saičić, Zorica",
year = "2018",
abstract = "During their complex life cycle, anurans are faced with various physiological and biochemical demands that can result in increased free radical production. The antioxidative system (AOS), assumes a central role in protection from oxidative stress, and increased knowledge of its response would allow us to identify and quantify underlying costs of free radical production. In this study, we compared the patterns and levels of integration of the AOS during two life stages of Bufotes viridis toads from natural populations: young, fully developed, reproductively inactive subadults and reproductively active adults. All examined parameters (SOD, CAT, GSH-Px, GR, GST, GSH and SH groups) were measured in the liver, skin and muscle. The obtained results showed that: (1) subadult individuals possessed a more integrated AOS (a higher index of integration and number of significant correlations), based mostly on the coordinated action of SOD-CAT; (2) adult individuals used the GSH-Px/GSH system; (3) all examined tissues exhibited similar patterns of variation of AOS parameters; (4) significant correlations between the AOS and biometric parameters. Our results indicate that adult and subadult toads differed in the way they processed free radicals, implying that adults may be more susceptible to oxidative damage. The knowledge from this study will contribute to a better understanding of the AOS and how its activity is modulated in natural populations of anurans throughout their life.",
journal = "Zoology",
title = "Comparative assessment of the antioxidative defense system in subadult and adult anurans: A lesson from the Bufotes viridis toad",
volume = "130",
doi = "10.1016/J.ZOOL.2018.08.001",
pages = "30-37"
}

Prokić, M., Petrović, T., Gavrić, J., Despotović, S., Gavrilović, B., Radovanović, T., Faggio, C.,& Saičić, Z.. (2018). Comparative assessment of the antioxidative defense system in subadult and adult anurans: A lesson from the Bufotes viridis toad. in Zoology, 130, 30-37.
https://doi.org/10.1016/J.ZOOL.2018.08.001

Prokić M, Petrović T, Gavrić J, Despotović S, Gavrilović B, Radovanović T, Faggio C, Saičić Z. Comparative assessment of the antioxidative defense system in subadult and adult anurans: A lesson from the Bufotes viridis toad. in Zoology. 2018;130:30-37.
doi:10.1016/J.ZOOL.2018.08.001 .

Prokić, Marko, Petrović, Tamara, Gavrić, Jelena, Despotović, Svetlana, Gavrilović, Branka, Radovanović, Tijana, Faggio, Caterina, Saičić, Zorica, "Comparative assessment of the antioxidative defense system in subadult and adult anurans: A lesson from the Bufotes viridis toad" in Zoology, 130 (2018):30-37,
https://doi.org/10.1016/J.ZOOL.2018.08.001 . .

TY  - JOUR
AU  - Nasia, Mohammed M.
AU  - Radovanović, Tijana
AU  - Krizmanić, Imre I.
AU  - Prokić, Marko
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Borković Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Saičić, Zorica
PY  - 2018
UR  - http://link.springer.com/10.1007/s11356-018-2979-x
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3142
AB  - Pesticide-induced oxidative stress, as one of mechanism of toxicity, has been a focus of toxicological research. However, there is a lack of data for certain pesticides-oxidative stress effects especially on terrestrial amphibians. This study evaluates the prooxidative effects of orally administered insecticide deltamethrin (DM) in some tissues of the terrestrial toad Bufotes viridis. Toads were randomly divided and assigned to a control group and a test group that was orally exposed to the pesticide (5 mg/kg of body weight/daily) for 21 days. Animals were euthanized from each group on days 7, 14, and 21, and the liver, leg muscle, ventral skin, and gastrointestinal tissue (GIT) were dissected and used for analysis. From battery of investigated antioxidant components, superoxide dismutase (SOD) was the most differentiate parameter in all examined tissues. For the period of prolonged exposure to pesticide, antioxidative strategy of Bufotes viridis was based on SOD utilization in attempt to maintain the oxidative disbalance at acceptable level. The integrated biomarker response (IBR) as the measure of the overall biochemical response to DM exposure revealed that the group exposed for 21 days had the highest response. Our work has offered valuable data ensuring evidence that toads exposed to deltamethrin developed adaptive reactions that were tissue-specific and that DM-generated systemic toxicity was time-dependent. The present work showed that oxidative stress has significant role in pesticide-induced toxicity and contributes to better understanding of ecotoxicological risk in the terrestrial amphibians exposed to DM.
T2  - Environmental Science and Pollution Research International
T1  - Prooxidant effects of chronic exposure to deltamethrin in green toad Bufotes viridis.
IS  - 30
VL  - 25
DO  - 10.1007/s11356-018-2979-x
SP  - 30597
EP  - 30608
ER  - 

@article{
author = "Nasia, Mohammed M. and Radovanović, Tijana and Krizmanić, Imre I. and Prokić, Marko and Gavrić, Jelena and Despotović, Svetlana and Gavrilović, Branka and Borković Mitić, Slavica and Pavlović, Slađan and Saičić, Zorica",
year = "2018",
abstract = "Pesticide-induced oxidative stress, as one of mechanism of toxicity, has been a focus of toxicological research. However, there is a lack of data for certain pesticides-oxidative stress effects especially on terrestrial amphibians. This study evaluates the prooxidative effects of orally administered insecticide deltamethrin (DM) in some tissues of the terrestrial toad Bufotes viridis. Toads were randomly divided and assigned to a control group and a test group that was orally exposed to the pesticide (5 mg/kg of body weight/daily) for 21 days. Animals were euthanized from each group on days 7, 14, and 21, and the liver, leg muscle, ventral skin, and gastrointestinal tissue (GIT) were dissected and used for analysis. From battery of investigated antioxidant components, superoxide dismutase (SOD) was the most differentiate parameter in all examined tissues. For the period of prolonged exposure to pesticide, antioxidative strategy of Bufotes viridis was based on SOD utilization in attempt to maintain the oxidative disbalance at acceptable level. The integrated biomarker response (IBR) as the measure of the overall biochemical response to DM exposure revealed that the group exposed for 21 days had the highest response. Our work has offered valuable data ensuring evidence that toads exposed to deltamethrin developed adaptive reactions that were tissue-specific and that DM-generated systemic toxicity was time-dependent. The present work showed that oxidative stress has significant role in pesticide-induced toxicity and contributes to better understanding of ecotoxicological risk in the terrestrial amphibians exposed to DM.",
journal = "Environmental Science and Pollution Research International",
title = "Prooxidant effects of chronic exposure to deltamethrin in green toad Bufotes viridis.",
number = "30",
volume = "25",
doi = "10.1007/s11356-018-2979-x",
pages = "30597-30608"
}

Nasia, M. M., Radovanović, T., Krizmanić, I. I., Prokić, M., Gavrić, J., Despotović, S., Gavrilović, B., Borković Mitić, S., Pavlović, S.,& Saičić, Z.. (2018). Prooxidant effects of chronic exposure to deltamethrin in green toad Bufotes viridis.. in Environmental Science and Pollution Research International, 25(30), 30597-30608.
https://doi.org/10.1007/s11356-018-2979-x

Nasia MM, Radovanović T, Krizmanić II, Prokić M, Gavrić J, Despotović S, Gavrilović B, Borković Mitić S, Pavlović S, Saičić Z. Prooxidant effects of chronic exposure to deltamethrin in green toad Bufotes viridis.. in Environmental Science and Pollution Research International. 2018;25(30):30597-30608.
doi:10.1007/s11356-018-2979-x .

Nasia, Mohammed M., Radovanović, Tijana, Krizmanić, Imre I., Prokić, Marko, Gavrić, Jelena, Despotović, Svetlana, Gavrilović, Branka, Borković Mitić, Slavica, Pavlović, Slađan, Saičić, Zorica, "Prooxidant effects of chronic exposure to deltamethrin in green toad Bufotes viridis." in Environmental Science and Pollution Research International, 25, no. 30 (2018):30597-30608,
https://doi.org/10.1007/s11356-018-2979-x . .

TY  - CONF
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Prokić, Marko
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Borković Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Saičić, Zorica
PY  - 2018
UR  - http://www.vbs.rs/scripts/cobiss?command=DISPLAY&base=99999&rid=267655948&fmt=11&lani=sc
UR  - http://www.serbiosoc.org.rs/wp-content/uploads/2018/11/DRUGI-KONGRES-BIOLOGA-SRBIJE-knjiga-sazetaka.pdf
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3416
AB  - Niske sredinske temperature tokom zimskih meseci predstavljaju izazov za zmije i druge ektotermne organizme. Prelazak u aktivno stanje u proleće zahteva povećanu potrošnju kiseonika što je u direktnoj vezi sa povećanom produkcijom reaktivnih vrsta kiseonika. Zato je bitno ispitati antioksidacione zaštitne mehanizme koji omogućavaju organizmima kod kojih dolazi do naglih promena u oksigenaciji tkiva da iz ovakvih stanja izađu bez oštećenja. Cilj ove studije bio je određivanje aktivnosti enzima zaštite od oksidacionih oštećenja: ukupne superoksid-dismutaze (SOD), katalaze (CAT), glutation-peroksidaze (GSH-Px), glutation-raduktaze (GR), enzima faze II biotransformacije glutation-S-transferaze (GST), koncentracije glutationa (GSH) i nivoa lipidnih peroksida (TBARS) u bubregu i masnom tkivu belouške (Natrix natrix) u periodu pre i posle hibernacije. Dobijeni rezultati su pokazali da posle hibernacije u bubregu ispitivane vrste dolazi do značajnog povećanja aktivnosti CAT kao i koncentracije GSH uz smanjenje aktivnosti GST. U masnom tkivu takođe je zabeleženo značajno povećanje aktivnosti SOD u proleće. U bubregu nisu zabeležene razlike u koncentraciji TBARS, dok je u masnom tkivu nivo lipidne peroksidacije značajno smanjen u periodu posle u odnosu na period pre hibernacije. Naši rezultati pokazuju da antioksidacioni zaštitni sistem N. natrix ima važnu ulogu u sprečavanju nastanka oksidacionih oštećenja tokom prelaska u aktivno stanje nakon perioda hibernacije.
PB  - Belgrade: Serbian Biological Society
C3  - Osnovna i primenjena istraživanja, metodika nastave : knjiga sažetaka / Drugi kongres biologa Srbije, Kladovo, Srbija 25-30.09.2018
T1  - Biomarkeri oksidacionog stresa u bubregu i masnom tkivu belouške (Natrix natrix) u periodu pre i posle hibernacije
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3416
ER  - 

@conference{
author = "Gavrić, Jelena and Despotović, Svetlana and Prokić, Marko and Gavrilović, Branka and Radovanović, Tijana and Borković Mitić, Slavica and Pavlović, Slađan and Saičić, Zorica",
year = "2018",
abstract = "Niske sredinske temperature tokom zimskih meseci predstavljaju izazov za zmije i druge ektotermne organizme. Prelazak u aktivno stanje u proleće zahteva povećanu potrošnju kiseonika što je u direktnoj vezi sa povećanom produkcijom reaktivnih vrsta kiseonika. Zato je bitno ispitati antioksidacione zaštitne mehanizme koji omogućavaju organizmima kod kojih dolazi do naglih promena u oksigenaciji tkiva da iz ovakvih stanja izađu bez oštećenja. Cilj ove studije bio je određivanje aktivnosti enzima zaštite od oksidacionih oštećenja: ukupne superoksid-dismutaze (SOD), katalaze (CAT), glutation-peroksidaze (GSH-Px), glutation-raduktaze (GR), enzima faze II biotransformacije glutation-S-transferaze (GST), koncentracije glutationa (GSH) i nivoa lipidnih peroksida (TBARS) u bubregu i masnom tkivu belouške (Natrix natrix) u periodu pre i posle hibernacije. Dobijeni rezultati su pokazali da posle hibernacije u bubregu ispitivane vrste dolazi do značajnog povećanja aktivnosti CAT kao i koncentracije GSH uz smanjenje aktivnosti GST. U masnom tkivu takođe je zabeleženo značajno povećanje aktivnosti SOD u proleće. U bubregu nisu zabeležene razlike u koncentraciji TBARS, dok je u masnom tkivu nivo lipidne peroksidacije značajno smanjen u periodu posle u odnosu na period pre hibernacije. Naši rezultati pokazuju da antioksidacioni zaštitni sistem N. natrix ima važnu ulogu u sprečavanju nastanka oksidacionih oštećenja tokom prelaska u aktivno stanje nakon perioda hibernacije.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Osnovna i primenjena istraživanja, metodika nastave : knjiga sažetaka / Drugi kongres biologa Srbije, Kladovo, Srbija 25-30.09.2018",
title = "Biomarkeri oksidacionog stresa u bubregu i masnom tkivu belouške (Natrix natrix) u periodu pre i posle hibernacije",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3416"
}

Gavrić, J., Despotović, S., Prokić, M., Gavrilović, B., Radovanović, T., Borković Mitić, S., Pavlović, S.,& Saičić, Z.. (2018). Biomarkeri oksidacionog stresa u bubregu i masnom tkivu belouške (Natrix natrix) u periodu pre i posle hibernacije. in Osnovna i primenjena istraživanja, metodika nastave : knjiga sažetaka / Drugi kongres biologa Srbije, Kladovo, Srbija 25-30.09.2018
Belgrade: Serbian Biological Society..
https://hdl.handle.net/21.15107/rcub_ibiss_3416

Gavrić J, Despotović S, Prokić M, Gavrilović B, Radovanović T, Borković Mitić S, Pavlović S, Saičić Z. Biomarkeri oksidacionog stresa u bubregu i masnom tkivu belouške (Natrix natrix) u periodu pre i posle hibernacije. in Osnovna i primenjena istraživanja, metodika nastave : knjiga sažetaka / Drugi kongres biologa Srbije, Kladovo, Srbija 25-30.09.2018. 2018;.
https://hdl.handle.net/21.15107/rcub_ibiss_3416 .

Gavrić, Jelena, Despotović, Svetlana, Prokić, Marko, Gavrilović, Branka, Radovanović, Tijana, Borković Mitić, Slavica, Pavlović, Slađan, Saičić, Zorica, "Biomarkeri oksidacionog stresa u bubregu i masnom tkivu belouške (Natrix natrix) u periodu pre i posle hibernacije" in Osnovna i primenjena istraživanja, metodika nastave : knjiga sažetaka / Drugi kongres biologa Srbije, Kladovo, Srbija 25-30.09.2018 (2018),
https://hdl.handle.net/21.15107/rcub_ibiss_3416 .

TY  - JOUR
AU  - Milošević, Marija D.
AU  - Paunović, Milica G.
AU  - Matić, Miloš M.
AU  - Ognjanović, Branka I.
AU  - Saičić, Zorica
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0753332218311636?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3100
AB  - Excessive use of organophosphate insecticides, including fenitrothion (FNT) can cause detrimental consequences in non-target organisms. Selenium (Se) and vitamin C (Vit C) possess protective abilities against various toxic compounds due to their antioxidative properties. Accordingly, the aim of the present study was to examine the possible ameliorative effects of Se and Vit C in hepatotoxicity induced by FNT. For the purpose of this study, male Wistar albino rats were divided into control and groups treated with Se (0.5 mg/kg b.w, as Na2SeO3) and Vit C (100 mg/kg b.w), FNT (20 mg/kg b.w) and FNT in cotreatment with Se and Vit C for 30 days. The current data showed a reduction in absolute and relative liver weight after FNT administration. Increased activities of liver enzymes (AST, ALT, ALP, LDH and GGT) indicated liver damage. FNT alone caused significant alterations in biochemical parameters (glucose and total bilirubin). Elevation in LPO level along with decreased activities of antioxidant enzymes (SOD, CAT, GSH-Px) and GSH content reflected the presence of oxidative stress. Coadministration of FNT with Se and Vit C exhibited hepatoprotective role confirmed by reduction of oxidative stress levels and restoration in the values of examined parameters. Because of their beneficial effects, Se and Vit C may be used in reducing injuries caused by pesticides.
T2  - Biomedicine & Pharmacotherapy
T1  - Role of selenium and vitamin C in mitigating oxidative stress induced by fenitrothion in rat liver.
VL  - 106
DO  - 10.1016/j.biopha.2018.06.132
SP  - 232
EP  - 238
ER  - 

@article{
author = "Milošević, Marija D. and Paunović, Milica G. and Matić, Miloš M. and Ognjanović, Branka I. and Saičić, Zorica",
year = "2018",
abstract = "Excessive use of organophosphate insecticides, including fenitrothion (FNT) can cause detrimental consequences in non-target organisms. Selenium (Se) and vitamin C (Vit C) possess protective abilities against various toxic compounds due to their antioxidative properties. Accordingly, the aim of the present study was to examine the possible ameliorative effects of Se and Vit C in hepatotoxicity induced by FNT. For the purpose of this study, male Wistar albino rats were divided into control and groups treated with Se (0.5 mg/kg b.w, as Na2SeO3) and Vit C (100 mg/kg b.w), FNT (20 mg/kg b.w) and FNT in cotreatment with Se and Vit C for 30 days. The current data showed a reduction in absolute and relative liver weight after FNT administration. Increased activities of liver enzymes (AST, ALT, ALP, LDH and GGT) indicated liver damage. FNT alone caused significant alterations in biochemical parameters (glucose and total bilirubin). Elevation in LPO level along with decreased activities of antioxidant enzymes (SOD, CAT, GSH-Px) and GSH content reflected the presence of oxidative stress. Coadministration of FNT with Se and Vit C exhibited hepatoprotective role confirmed by reduction of oxidative stress levels and restoration in the values of examined parameters. Because of their beneficial effects, Se and Vit C may be used in reducing injuries caused by pesticides.",
journal = "Biomedicine & Pharmacotherapy",
title = "Role of selenium and vitamin C in mitigating oxidative stress induced by fenitrothion in rat liver.",
volume = "106",
doi = "10.1016/j.biopha.2018.06.132",
pages = "232-238"
}

Milošević, M. D., Paunović, M. G., Matić, M. M., Ognjanović, B. I.,& Saičić, Z.. (2018). Role of selenium and vitamin C in mitigating oxidative stress induced by fenitrothion in rat liver.. in Biomedicine & Pharmacotherapy, 106, 232-238.
https://doi.org/10.1016/j.biopha.2018.06.132

Milošević MD, Paunović MG, Matić MM, Ognjanović BI, Saičić Z. Role of selenium and vitamin C in mitigating oxidative stress induced by fenitrothion in rat liver.. in Biomedicine & Pharmacotherapy. 2018;106:232-238.
doi:10.1016/j.biopha.2018.06.132 .

Milošević, Marija D., Paunović, Milica G., Matić, Miloš M., Ognjanović, Branka I., Saičić, Zorica, "Role of selenium and vitamin C in mitigating oxidative stress induced by fenitrothion in rat liver." in Biomedicine & Pharmacotherapy, 106 (2018):232-238,
https://doi.org/10.1016/j.biopha.2018.06.132 . .

TY  - JOUR
AU  - Prokić, Marko
AU  - Despotović, Svetlana
AU  - Vučić, Tijana
AU  - Petrović, Tamara
AU  - Gavrić, Jelena
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Saičić, Zorica
PY  - 2018
UR  - http://jeb.biologists.org/content/early/2018/08/17/jeb.182055
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3151
AB  - Oxidative stress has most recently been suggested as one of possible mechanisms responsible for reduced fitness of hybrids. To explore possible oxidative cost of hybridization, we examined antioxidant defense system parameters (superoxide dismutase, catalase, glutathione peroxidase, glutathione s-transferase, glutathione reductase, glutathione, SH groups), their interconnectedness (index of integration), and levels of oxidative damage (concentrations of lipid peroxides-TBARS) in lab-reared newt species, Triturus macedonicus and T. ivanbureschi, and their hybrid. Our results showed that parental species differed in antioxidant defense system parameters, but not in the levels of integration of the whole system and oxidative damage. Individuals of T. ivanbureschi had higher activities of superoxide dismutase, glutathione s-transferase and concentrations of glutathione. Hybrid individuals of crested newts displayed higher levels of the antioxidant defense system (higher superoxide dismutase, catalase, glutathione peroxidase activities and concentrations of SH groups), and a lower overall correlation of antioxidant system (lower index of integration) in comparison to both parental species, suggesting that they may possess a less efficient antioxidant defense system and a higher investment in maintaining oxidative balance. The higher investment in the antioxidant system could divert limited resources away from other functions and affect further hybrid fitness. The presented findings contribute to a better understanding of the antioxidant defense system of crested newts and their interspecies differences, and support the hypothesis that oxidative stress is one of the costs of interspecific hybridization.
T2  - The Journal of Experimental Biology
T1  - Oxidative cost of interspecific hybridization: a case study of two Triturus species and their hybrids.
DO  - 10.1242/jeb.182055
ER  - 

@article{
author = "Prokić, Marko and Despotović, Svetlana and Vučić, Tijana and Petrović, Tamara and Gavrić, Jelena and Gavrilović, Branka and Radovanović, Tijana and Saičić, Zorica",
year = "2018",
abstract = "Oxidative stress has most recently been suggested as one of possible mechanisms responsible for reduced fitness of hybrids. To explore possible oxidative cost of hybridization, we examined antioxidant defense system parameters (superoxide dismutase, catalase, glutathione peroxidase, glutathione s-transferase, glutathione reductase, glutathione, SH groups), their interconnectedness (index of integration), and levels of oxidative damage (concentrations of lipid peroxides-TBARS) in lab-reared newt species, Triturus macedonicus and T. ivanbureschi, and their hybrid. Our results showed that parental species differed in antioxidant defense system parameters, but not in the levels of integration of the whole system and oxidative damage. Individuals of T. ivanbureschi had higher activities of superoxide dismutase, glutathione s-transferase and concentrations of glutathione. Hybrid individuals of crested newts displayed higher levels of the antioxidant defense system (higher superoxide dismutase, catalase, glutathione peroxidase activities and concentrations of SH groups), and a lower overall correlation of antioxidant system (lower index of integration) in comparison to both parental species, suggesting that they may possess a less efficient antioxidant defense system and a higher investment in maintaining oxidative balance. The higher investment in the antioxidant system could divert limited resources away from other functions and affect further hybrid fitness. The presented findings contribute to a better understanding of the antioxidant defense system of crested newts and their interspecies differences, and support the hypothesis that oxidative stress is one of the costs of interspecific hybridization.",
journal = "The Journal of Experimental Biology",
title = "Oxidative cost of interspecific hybridization: a case study of two Triturus species and their hybrids.",
doi = "10.1242/jeb.182055"
}

Prokić, M., Despotović, S., Vučić, T., Petrović, T., Gavrić, J., Gavrilović, B., Radovanović, T.,& Saičić, Z.. (2018). Oxidative cost of interspecific hybridization: a case study of two Triturus species and their hybrids.. in The Journal of Experimental Biology.
https://doi.org/10.1242/jeb.182055

Prokić M, Despotović S, Vučić T, Petrović T, Gavrić J, Gavrilović B, Radovanović T, Saičić Z. Oxidative cost of interspecific hybridization: a case study of two Triturus species and their hybrids.. in The Journal of Experimental Biology. 2018;.
doi:10.1242/jeb.182055 .

Prokić, Marko, Despotović, Svetlana, Vučić, Tijana, Petrović, Tamara, Gavrić, Jelena, Gavrilović, Branka, Radovanović, Tijana, Saičić, Zorica, "Oxidative cost of interspecific hybridization: a case study of two Triturus species and their hybrids." in The Journal of Experimental Biology (2018),
https://doi.org/10.1242/jeb.182055 . .

TY  - JOUR
AU  - Prokić, Marko
AU  - Despotović, Svetlana
AU  - Vučić, Tijana
AU  - Petrović, Tamara
AU  - Gavrić, Jelena
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Saičić, Zorica
PY  - 2018
UR  - http://jeb.biologists.org/content/early/2018/08/17/jeb.182055
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3160
AB  - Oxidative stress has most recently been suggested as one of the possible mechanisms responsible for reduced fitness of hybrids. To explore possible oxidative cost of hybridization, we examined anti-oxidant defence system parameters (superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase, glutathione reductase, glutathione, SH groups), their interconnectedness (index of integration) and levels of oxidative damage [concentrations of lipid peroxides, TBARS (thiobarbituric acid reactive substances)] in laboratory-reared newt species, Triturus macedonicus and T. ivanbureschi, and their hybrid. Our results showed that parental species differed in anti-oxidant defence system parameters, but not in the levels of integration of the whole system and oxidative damage. Individuals of T. ivanbureschi had higher activities of superoxide dismutase, glutathione S-transferase and concentrations of glutathione. Hybrid individuals of crested newts displayed higher levels of the anti-oxidant defence system (higher superoxide dismutase, catalase, glutathione peroxidase activities and concentrations of SH groups), and a lower overall correlation of anti-oxidant system (lower index of integration) in comparison with both parental species, suggesting that they may possess a less efficient anti-oxidant defence system and a higher investment in maintaining oxidative balance. The higher investment in the anti-oxidant system could divert limited resources away from other functions and affect further hybrid fitness. The presented findings contribute to a better understanding of the anti-oxidant defence system of crested newts and their interspecies differences, and support the hypothesis that oxidative stress is one of the costs of interspecific hybridization.
T2  - The Journal of Experimental Biology
T1  - Oxidative cost of interspecific hybridization: a case study of two Triturus species and their hybrids.
IS  - Pt 19
VL  - 221
DO  - 10.1242/jeb.182055
SP  - jeb182055
ER  - 

@article{
author = "Prokić, Marko and Despotović, Svetlana and Vučić, Tijana and Petrović, Tamara and Gavrić, Jelena and Gavrilović, Branka and Radovanović, Tijana and Saičić, Zorica",
year = "2018",
abstract = "Oxidative stress has most recently been suggested as one of the possible mechanisms responsible for reduced fitness of hybrids. To explore possible oxidative cost of hybridization, we examined anti-oxidant defence system parameters (superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase, glutathione reductase, glutathione, SH groups), their interconnectedness (index of integration) and levels of oxidative damage [concentrations of lipid peroxides, TBARS (thiobarbituric acid reactive substances)] in laboratory-reared newt species, Triturus macedonicus and T. ivanbureschi, and their hybrid. Our results showed that parental species differed in anti-oxidant defence system parameters, but not in the levels of integration of the whole system and oxidative damage. Individuals of T. ivanbureschi had higher activities of superoxide dismutase, glutathione S-transferase and concentrations of glutathione. Hybrid individuals of crested newts displayed higher levels of the anti-oxidant defence system (higher superoxide dismutase, catalase, glutathione peroxidase activities and concentrations of SH groups), and a lower overall correlation of anti-oxidant system (lower index of integration) in comparison with both parental species, suggesting that they may possess a less efficient anti-oxidant defence system and a higher investment in maintaining oxidative balance. The higher investment in the anti-oxidant system could divert limited resources away from other functions and affect further hybrid fitness. The presented findings contribute to a better understanding of the anti-oxidant defence system of crested newts and their interspecies differences, and support the hypothesis that oxidative stress is one of the costs of interspecific hybridization.",
journal = "The Journal of Experimental Biology",
title = "Oxidative cost of interspecific hybridization: a case study of two Triturus species and their hybrids.",
number = "Pt 19",
volume = "221",
doi = "10.1242/jeb.182055",
pages = "jeb182055"
}

Prokić, M., Despotović, S., Vučić, T., Petrović, T., Gavrić, J., Gavrilović, B., Radovanović, T.,& Saičić, Z.. (2018). Oxidative cost of interspecific hybridization: a case study of two Triturus species and their hybrids.. in The Journal of Experimental Biology, 221(Pt 19), jeb182055.
https://doi.org/10.1242/jeb.182055

Prokić M, Despotović S, Vučić T, Petrović T, Gavrić J, Gavrilović B, Radovanović T, Saičić Z. Oxidative cost of interspecific hybridization: a case study of two Triturus species and their hybrids.. in The Journal of Experimental Biology. 2018;221(Pt 19):jeb182055.
doi:10.1242/jeb.182055 .

Prokić, Marko, Despotović, Svetlana, Vučić, Tijana, Petrović, Tamara, Gavrić, Jelena, Gavrilović, Branka, Radovanović, Tijana, Saičić, Zorica, "Oxidative cost of interspecific hybridization: a case study of two Triturus species and their hybrids." in The Journal of Experimental Biology, 221, no. Pt 19 (2018):jeb182055,
https://doi.org/10.1242/jeb.182055 . .

TY  - JOUR
AU  - Matić, Miloš
AU  - Paunović, Milica
AU  - Ognjanović, Branka
AU  - Štajn, Andraš
AU  - Saičić, Zorica
PY  - 2017
UR  - internal-pdf://Matić et al. - 2016 - Neuropeptide Y reduces migration capacity of human choriocarcinoma cell line by altering oxidative antioxidative.pdf
UR  - http://online.journals.tubitak.gov.tr/openInPressDocument.htm?fileID=799024&no=150073&fileType=Report Document
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2614
AB  - Reduced migration capacity of trophoblast cells leads to poor placentation and correlates with severe pregnancy disorders such as intrauterine growth restriction and preeclampsia. Neuropeptide Y (NPY) is sympathetic cotransmitter involved in various physiological processes and its levels are significantly increased in preeclamptic pregnancy compared to healthy pregnancy. In this study the prooxidative role of NPY and its effects on migration capacity of human trophoblast cell line JEG-3 were investigated together with the effects of nitric oxide (NO) depletion, a molecule that was shown to play an important role in promoting cell migration. The cells were treated for 24 h (short-term stimulation) and 72 h (long-term stimulation) respectively with 1 nM NPY. Oxidative/antioxidative status and the migration index of cells were measured. The results showed increased concentrations of oxidative stress parameters (O2 •–, H2O2) and molecules of the antioxidant defense system (reduced glutathione and oxidized glutathione), while the levels of intracellular nitrites (indicators of NO) and cell migration index were significantly decreased in trophoblast cells treated with NPY (both at 24 h and 72 h of exposure) compared to the control cells. These results suggest that NPY may significantly contribute to reduced migration capacity of trophoblast cells by generating oxidative stress and reducing the bioavailability of NO.
T2  - Turkish Journal of Biology
T1  - Neuropeptide Y reduces migration capacity of human choriocarcinoma cell line by altering oxidative / antioxidative status
VL  - 41
DO  - 10.3906/biy-1606-73
SP  - 292
EP  - 301
ER  - 

@article{
author = "Matić, Miloš and Paunović, Milica and Ognjanović, Branka and Štajn, Andraš and Saičić, Zorica",
year = "2017",
abstract = "Reduced migration capacity of trophoblast cells leads to poor placentation and correlates with severe pregnancy disorders such as intrauterine growth restriction and preeclampsia. Neuropeptide Y (NPY) is sympathetic cotransmitter involved in various physiological processes and its levels are significantly increased in preeclamptic pregnancy compared to healthy pregnancy. In this study the prooxidative role of NPY and its effects on migration capacity of human trophoblast cell line JEG-3 were investigated together with the effects of nitric oxide (NO) depletion, a molecule that was shown to play an important role in promoting cell migration. The cells were treated for 24 h (short-term stimulation) and 72 h (long-term stimulation) respectively with 1 nM NPY. Oxidative/antioxidative status and the migration index of cells were measured. The results showed increased concentrations of oxidative stress parameters (O2 •–, H2O2) and molecules of the antioxidant defense system (reduced glutathione and oxidized glutathione), while the levels of intracellular nitrites (indicators of NO) and cell migration index were significantly decreased in trophoblast cells treated with NPY (both at 24 h and 72 h of exposure) compared to the control cells. These results suggest that NPY may significantly contribute to reduced migration capacity of trophoblast cells by generating oxidative stress and reducing the bioavailability of NO.",
journal = "Turkish Journal of Biology",
title = "Neuropeptide Y reduces migration capacity of human choriocarcinoma cell line by altering oxidative / antioxidative status",
volume = "41",
doi = "10.3906/biy-1606-73",
pages = "292-301"
}

Matić, M., Paunović, M., Ognjanović, B., Štajn, A.,& Saičić, Z.. (2017). Neuropeptide Y reduces migration capacity of human choriocarcinoma cell line by altering oxidative / antioxidative status. in Turkish Journal of Biology, 41, 292-301.
https://doi.org/10.3906/biy-1606-73

Matić M, Paunović M, Ognjanović B, Štajn A, Saičić Z. Neuropeptide Y reduces migration capacity of human choriocarcinoma cell line by altering oxidative / antioxidative status. in Turkish Journal of Biology. 2017;41:292-301.
doi:10.3906/biy-1606-73 .

Matić, Miloš, Paunović, Milica, Ognjanović, Branka, Štajn, Andraš, Saičić, Zorica, "Neuropeptide Y reduces migration capacity of human choriocarcinoma cell line by altering oxidative / antioxidative status" in Turkish Journal of Biology, 41 (2017):292-301,
https://doi.org/10.3906/biy-1606-73 . .

TY  - JOUR
AU  - Matić, Miloš
AU  - Paunović, Milica
AU  - Ognjanović, Branka
AU  - Štajn, Andraš
AU  - Saičić, Zorica
PY  - 2017
UR  - http://online.journals.tubitak.gov.tr/openInPressDocument.htm?fileID=799024&no=150073&fileType=Report Document
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2739
AB  - Reduced migration capacity of trophoblast cells leads to poor placentation and correlates with severe pregnancy disorders such as intrauterine growth restriction and preeclampsia. Neuropeptide Y (NPY) is sympathetic cotransmitter involved in various physiological processes and its levels are significantly increased in preeclamptic pregnancy compared to healthy pregnancy. In this study the prooxidative role of NPY and its effects on migration capacity of human trophoblast cell line JEG-3 were investigated together with the effects of nitric oxide (NO) depletion, a molecule that was shown to play an important role in promoting cell migration. The cells were treated for 24 h (short-term stimulation) and 72 h (long-term stimulation) respectively with 1 nM NPY. Oxidative/antioxidative status and the migration index of cells were measured. The results showed increased concentrations of oxidative stress parameters (O 2 •– , H 2 O 2 ) and molecules of the antioxidant defense system (reduced glutathione and oxidized glutathione), while the levels of intracellular nitrites (indicators of NO) and cell migration index were significantly decreased in trophoblast cells treated with NPY (both at 24 h and 72 h of exposure) compared to the control cells. These results suggest that NPY may significantly contribute to reduced migration capacity of trophoblast cells by generating oxidative stress and reducing the bioavailability of NO.
T2  - Turkish Journal of Biology
T1  - Neuropeptide Y reduces migration capacity of human choriocarcinoma cell line by altering oxidative / antioxidative status
IS  - 2
VL  - 41
DO  - 10.3906/biy-1606-73
SP  - 292
EP  - 301
ER  - 

@article{
author = "Matić, Miloš and Paunović, Milica and Ognjanović, Branka and Štajn, Andraš and Saičić, Zorica",
year = "2017",
abstract = "Reduced migration capacity of trophoblast cells leads to poor placentation and correlates with severe pregnancy disorders such as intrauterine growth restriction and preeclampsia. Neuropeptide Y (NPY) is sympathetic cotransmitter involved in various physiological processes and its levels are significantly increased in preeclamptic pregnancy compared to healthy pregnancy. In this study the prooxidative role of NPY and its effects on migration capacity of human trophoblast cell line JEG-3 were investigated together with the effects of nitric oxide (NO) depletion, a molecule that was shown to play an important role in promoting cell migration. The cells were treated for 24 h (short-term stimulation) and 72 h (long-term stimulation) respectively with 1 nM NPY. Oxidative/antioxidative status and the migration index of cells were measured. The results showed increased concentrations of oxidative stress parameters (O 2 •– , H 2 O 2 ) and molecules of the antioxidant defense system (reduced glutathione and oxidized glutathione), while the levels of intracellular nitrites (indicators of NO) and cell migration index were significantly decreased in trophoblast cells treated with NPY (both at 24 h and 72 h of exposure) compared to the control cells. These results suggest that NPY may significantly contribute to reduced migration capacity of trophoblast cells by generating oxidative stress and reducing the bioavailability of NO.",
journal = "Turkish Journal of Biology",
title = "Neuropeptide Y reduces migration capacity of human choriocarcinoma cell line by altering oxidative / antioxidative status",
number = "2",
volume = "41",
doi = "10.3906/biy-1606-73",
pages = "292-301"
}

Matić, M., Paunović, M., Ognjanović, B., Štajn, A.,& Saičić, Z.. (2017). Neuropeptide Y reduces migration capacity of human choriocarcinoma cell line by altering oxidative / antioxidative status. in Turkish Journal of Biology, 41(2), 292-301.
https://doi.org/10.3906/biy-1606-73

Matić M, Paunović M, Ognjanović B, Štajn A, Saičić Z. Neuropeptide Y reduces migration capacity of human choriocarcinoma cell line by altering oxidative / antioxidative status. in Turkish Journal of Biology. 2017;41(2):292-301.
doi:10.3906/biy-1606-73 .

Matić, Miloš, Paunović, Milica, Ognjanović, Branka, Štajn, Andraš, Saičić, Zorica, "Neuropeptide Y reduces migration capacity of human choriocarcinoma cell line by altering oxidative / antioxidative status" in Turkish Journal of Biology, 41, no. 2 (2017):292-301,
https://doi.org/10.3906/biy-1606-73 . .

TY  - JOUR
AU  - Radovanović, Tijana
AU  - Nasia, Mohammed
AU  - Krizmanić, Imre I.
AU  - Prokić, Marko
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Borković Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Saičić, Zorica
PY  - 2017
UR  - http://doi.wiley.com/10.1002/etc.3849
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3418
AB  - Pyrethroids are synthetic insecticides widely used in agriculture, public health, and veterinary medicine. Deltamethrin, a type II pyrethroid insecticide, has attracted particular attention because of its frequent use. The mechanisms of the toxicity of most pesticides (including pyrethroids) in nontarget organisms is linked to the production of free radicals, oxidative stress induction, increased lipid peroxidation, and disruption of the total antioxidant potential. The aim of the present study was to investigate the effects of acute toxicity of 3 different concentrations (8, 16, and 32 mg/kg body wt) of orally applied deltamethrin after 96 h of treatment. Some of the front-line oxidative stress parameters as well as cholinesterase (ChE) activity and cytochrome P4501A (CYP1A) expression in the liver, muscle, skin, and gastrointestinal tissue of the adult green toad Bufotes viridis were determined. Compared with the control group, the activity of catalase and glutathione reductase was increased in the liver and skin, while the concentration of sulfhydryl groups was reduced. In the liver and muscle, concentrations of thiobarbituric reactive substances were increased, as well as liver CYP1A expression. In the muscle and skin, glutathione-S-transferase activity was higher in treated toads. The oxidative stress parameters examined were affected by different deltamethrin concentrations. We conclude that the assessed parameters represent good biomarkers of pesticide-induced oxidative stress. Environ Toxicol Chem 2017;36:2814-2822. © 2017 SETAC.
T2  - Environmental Toxicology and Chemistry
T1  - Sublethal effects of the pyrethroid insecticide deltamethrin on oxidative stress parameters in green toad (Bufotes viridis L.).
IS  - 10
VL  - 36
DO  - 10.1002/etc.3849
SP  - 2814
EP  - 2822
ER  - 

@article{
author = "Radovanović, Tijana and Nasia, Mohammed and Krizmanić, Imre I. and Prokić, Marko and Gavrić, Jelena and Despotović, Svetlana and Gavrilović, Branka and Borković Mitić, Slavica and Pavlović, Slađan and Saičić, Zorica",
year = "2017",
abstract = "Pyrethroids are synthetic insecticides widely used in agriculture, public health, and veterinary medicine. Deltamethrin, a type II pyrethroid insecticide, has attracted particular attention because of its frequent use. The mechanisms of the toxicity of most pesticides (including pyrethroids) in nontarget organisms is linked to the production of free radicals, oxidative stress induction, increased lipid peroxidation, and disruption of the total antioxidant potential. The aim of the present study was to investigate the effects of acute toxicity of 3 different concentrations (8, 16, and 32 mg/kg body wt) of orally applied deltamethrin after 96 h of treatment. Some of the front-line oxidative stress parameters as well as cholinesterase (ChE) activity and cytochrome P4501A (CYP1A) expression in the liver, muscle, skin, and gastrointestinal tissue of the adult green toad Bufotes viridis were determined. Compared with the control group, the activity of catalase and glutathione reductase was increased in the liver and skin, while the concentration of sulfhydryl groups was reduced. In the liver and muscle, concentrations of thiobarbituric reactive substances were increased, as well as liver CYP1A expression. In the muscle and skin, glutathione-S-transferase activity was higher in treated toads. The oxidative stress parameters examined were affected by different deltamethrin concentrations. We conclude that the assessed parameters represent good biomarkers of pesticide-induced oxidative stress. Environ Toxicol Chem 2017;36:2814-2822. © 2017 SETAC.",
journal = "Environmental Toxicology and Chemistry",
title = "Sublethal effects of the pyrethroid insecticide deltamethrin on oxidative stress parameters in green toad (Bufotes viridis L.).",
number = "10",
volume = "36",
doi = "10.1002/etc.3849",
pages = "2814-2822"
}

Radovanović, T., Nasia, M., Krizmanić, I. I., Prokić, M., Gavrić, J., Despotović, S., Gavrilović, B., Borković Mitić, S., Pavlović, S.,& Saičić, Z.. (2017). Sublethal effects of the pyrethroid insecticide deltamethrin on oxidative stress parameters in green toad (Bufotes viridis L.).. in Environmental Toxicology and Chemistry, 36(10), 2814-2822.
https://doi.org/10.1002/etc.3849

Radovanović T, Nasia M, Krizmanić II, Prokić M, Gavrić J, Despotović S, Gavrilović B, Borković Mitić S, Pavlović S, Saičić Z. Sublethal effects of the pyrethroid insecticide deltamethrin on oxidative stress parameters in green toad (Bufotes viridis L.).. in Environmental Toxicology and Chemistry. 2017;36(10):2814-2822.
doi:10.1002/etc.3849 .

Radovanović, Tijana, Nasia, Mohammed, Krizmanić, Imre I., Prokić, Marko, Gavrić, Jelena, Despotović, Svetlana, Gavrilović, Branka, Borković Mitić, Slavica, Pavlović, Slađan, Saičić, Zorica, "Sublethal effects of the pyrethroid insecticide deltamethrin on oxidative stress parameters in green toad (Bufotes viridis L.)." in Environmental Toxicology and Chemistry, 36, no. 10 (2017):2814-2822,
https://doi.org/10.1002/etc.3849 . .