TY  - JOUR
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Petrović, Tamara
AU  - Radovanović, Tijana
AU  - Gavrić, Jelena
AU  - Mirč, Marko
AU  - Anđelković, Marko
AU  - Vukov, Tanja
AU  - Tomašević Kolarov, Nataša
AU  - Prokić, Marko
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6580
AB  - The effect of anesthesia/euthanasia with ethyl 3-aminobenzoate methanesulfonate (MS-222) on the oxidative status of Hyla arborea tadpoles was examined to determine whether the use of the anesthetic can confound the experimental results of the oxidative stress-based investigation. The experiment was conducted on two groups of tadpoles reared at different temperatures to produce differences in antioxidant capacity between the groups. After development at different temperatures (20 °C and 25 °C), the animals were exposed to different concentrations of MS-222 (0, 0.1, 1, and 5 g/L) for 15 min. The higher temperature decreased catalase activity, glutathione and protein carbonyl levels and increased glutathione reductase activity. The glutathione level and glutathione/thiol-related parameters were significantly changed after MS-222 exposure. However, individuals from the different temperature groups responded differently to the tested anesthetic, pointing to the possible influence of the initial levels of antioxidant capacity. The analysis of the interaction between the factors (temperature and MS-222) confirmed that the anesthetic can confound the results regarding the effects of temperature on the oxidative status parameters. The concentration of 0.1 g/L MS-222 had the lowest influence on the alterations in oxidative status and the results of the effect of temperature. A brief review of the current literature on the use of MS-222 in tadpoles made clear the absence of precise information on anesthetic concentration and exposure time. Similar studies should be repeated and extended to other amphibian species and other factors of interest to provide better guidance on tadpole anesthesia/euthanasia for future experiments that consider oxidative status parameters.
PB  - Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Does the anesthetic tricaine methanesulfonate (MS-222) distort oxidative status parameters in tadpoles?
VL  - 278
DO  - 10.1016/j.cbpc.2024.109859
SP  - 109859
ER  - 

@article{
author = "Gavrilović, Branka and Despotović, Svetlana and Petrović, Tamara and Radovanović, Tijana and Gavrić, Jelena and Mirč, Marko and Anđelković, Marko and Vukov, Tanja and Tomašević Kolarov, Nataša and Prokić, Marko",
year = "2024",
abstract = "The effect of anesthesia/euthanasia with ethyl 3-aminobenzoate methanesulfonate (MS-222) on the oxidative status of Hyla arborea tadpoles was examined to determine whether the use of the anesthetic can confound the experimental results of the oxidative stress-based investigation. The experiment was conducted on two groups of tadpoles reared at different temperatures to produce differences in antioxidant capacity between the groups. After development at different temperatures (20 °C and 25 °C), the animals were exposed to different concentrations of MS-222 (0, 0.1, 1, and 5 g/L) for 15 min. The higher temperature decreased catalase activity, glutathione and protein carbonyl levels and increased glutathione reductase activity. The glutathione level and glutathione/thiol-related parameters were significantly changed after MS-222 exposure. However, individuals from the different temperature groups responded differently to the tested anesthetic, pointing to the possible influence of the initial levels of antioxidant capacity. The analysis of the interaction between the factors (temperature and MS-222) confirmed that the anesthetic can confound the results regarding the effects of temperature on the oxidative status parameters. The concentration of 0.1 g/L MS-222 had the lowest influence on the alterations in oxidative status and the results of the effect of temperature. A brief review of the current literature on the use of MS-222 in tadpoles made clear the absence of precise information on anesthetic concentration and exposure time. Similar studies should be repeated and extended to other amphibian species and other factors of interest to provide better guidance on tadpole anesthesia/euthanasia for future experiments that consider oxidative status parameters.",
publisher = "Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Does the anesthetic tricaine methanesulfonate (MS-222) distort oxidative status parameters in tadpoles?",
volume = "278",
doi = "10.1016/j.cbpc.2024.109859",
pages = "109859"
}

Gavrilović, B., Despotović, S., Petrović, T., Radovanović, T., Gavrić, J., Mirč, M., Anđelković, M., Vukov, T., Tomašević Kolarov, N.,& Prokić, M.. (2024). Does the anesthetic tricaine methanesulfonate (MS-222) distort oxidative status parameters in tadpoles?. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
Elsevier Inc.., 278, 109859.
https://doi.org/10.1016/j.cbpc.2024.109859

Gavrilović B, Despotović S, Petrović T, Radovanović T, Gavrić J, Mirč M, Anđelković M, Vukov T, Tomašević Kolarov N, Prokić M. Does the anesthetic tricaine methanesulfonate (MS-222) distort oxidative status parameters in tadpoles?. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2024;278:109859.
doi:10.1016/j.cbpc.2024.109859 .

Gavrilović, Branka, Despotović, Svetlana, Petrović, Tamara, Radovanović, Tijana, Gavrić, Jelena, Mirč, Marko, Anđelković, Marko, Vukov, Tanja, Tomašević Kolarov, Nataša, Prokić, Marko, "Does the anesthetic tricaine methanesulfonate (MS-222) distort oxidative status parameters in tadpoles?" in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 278 (2024):109859,
https://doi.org/10.1016/j.cbpc.2024.109859 . .

TY  - JOUR
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Petrović, Tamara
AU  - Radovanović, Tijana
AU  - Gavrić, Jelena
AU  - Mirč, Marko
AU  - Anđelković, Marko
AU  - Vukov, Tanja
AU  - Tomašević Kolarov, Nataša
AU  - Prokić, Marko
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6579
AB  - The effect of anesthesia/euthanasia with ethyl 3-aminobenzoate methanesulfonate (MS-222) on the oxidative status of Hyla arborea tadpoles was examined to determine whether the use of the anesthetic can confound the experimental results of the oxidative stress-based investigation. The experiment was conducted on two groups of tadpoles reared at different temperatures to produce differences in antioxidant capacity between the groups. After development at different temperatures (20 °C and 25 °C), the animals were exposed to different concentrations of MS-222 (0, 0.1, 1, and 5 g/L) for 15 min. The higher temperature decreased catalase activity, glutathione and protein carbonyl levels and increased glutathione reductase activity. The glutathione level and glutathione/thiol-related parameters were significantly changed after MS-222 exposure. However, individuals from the different temperature groups responded differently to the tested anesthetic, pointing to the possible influence of the initial levels of antioxidant capacity. The analysis of the interaction between the factors (temperature and MS-222) confirmed that the anesthetic can confound the results regarding the effects of temperature on the oxidative status parameters. The concentration of 0.1 g/L MS-222 had the lowest influence on the alterations in oxidative status and the results of the effect of temperature. A brief review of the current literature on the use of MS-222 in tadpoles made clear the absence of precise information on anesthetic concentration and exposure time. Similar studies should be repeated and extended to other amphibian species and other factors of interest to provide better guidance on tadpole anesthesia/euthanasia for future experiments that consider oxidative status parameters.
PB  - Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Does the anesthetic tricaine methanesulfonate (MS-222) distort oxidative status parameters in tadpoles?
VL  - 278
DO  - 10.1016/j.cbpc.2024.109859
SP  - 109859
ER  - 

@article{
author = "Gavrilović, Branka and Despotović, Svetlana and Petrović, Tamara and Radovanović, Tijana and Gavrić, Jelena and Mirč, Marko and Anđelković, Marko and Vukov, Tanja and Tomašević Kolarov, Nataša and Prokić, Marko",
year = "2024",
abstract = "The effect of anesthesia/euthanasia with ethyl 3-aminobenzoate methanesulfonate (MS-222) on the oxidative status of Hyla arborea tadpoles was examined to determine whether the use of the anesthetic can confound the experimental results of the oxidative stress-based investigation. The experiment was conducted on two groups of tadpoles reared at different temperatures to produce differences in antioxidant capacity between the groups. After development at different temperatures (20 °C and 25 °C), the animals were exposed to different concentrations of MS-222 (0, 0.1, 1, and 5 g/L) for 15 min. The higher temperature decreased catalase activity, glutathione and protein carbonyl levels and increased glutathione reductase activity. The glutathione level and glutathione/thiol-related parameters were significantly changed after MS-222 exposure. However, individuals from the different temperature groups responded differently to the tested anesthetic, pointing to the possible influence of the initial levels of antioxidant capacity. The analysis of the interaction between the factors (temperature and MS-222) confirmed that the anesthetic can confound the results regarding the effects of temperature on the oxidative status parameters. The concentration of 0.1 g/L MS-222 had the lowest influence on the alterations in oxidative status and the results of the effect of temperature. A brief review of the current literature on the use of MS-222 in tadpoles made clear the absence of precise information on anesthetic concentration and exposure time. Similar studies should be repeated and extended to other amphibian species and other factors of interest to provide better guidance on tadpole anesthesia/euthanasia for future experiments that consider oxidative status parameters.",
publisher = "Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Does the anesthetic tricaine methanesulfonate (MS-222) distort oxidative status parameters in tadpoles?",
volume = "278",
doi = "10.1016/j.cbpc.2024.109859",
pages = "109859"
}

Gavrilović, B., Despotović, S., Petrović, T., Radovanović, T., Gavrić, J., Mirč, M., Anđelković, M., Vukov, T., Tomašević Kolarov, N.,& Prokić, M.. (2024). Does the anesthetic tricaine methanesulfonate (MS-222) distort oxidative status parameters in tadpoles?. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
Elsevier Inc.., 278, 109859.
https://doi.org/10.1016/j.cbpc.2024.109859

Gavrilović B, Despotović S, Petrović T, Radovanović T, Gavrić J, Mirč M, Anđelković M, Vukov T, Tomašević Kolarov N, Prokić M. Does the anesthetic tricaine methanesulfonate (MS-222) distort oxidative status parameters in tadpoles?. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2024;278:109859.
doi:10.1016/j.cbpc.2024.109859 .

Gavrilović, Branka, Despotović, Svetlana, Petrović, Tamara, Radovanović, Tijana, Gavrić, Jelena, Mirč, Marko, Anđelković, Marko, Vukov, Tanja, Tomašević Kolarov, Nataša, Prokić, Marko, "Does the anesthetic tricaine methanesulfonate (MS-222) distort oxidative status parameters in tadpoles?" in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 278 (2024):109859,
https://doi.org/10.1016/j.cbpc.2024.109859 . .

TY  - JOUR
AU  - Morshedi, Vahid
AU  - Noori, Farzaneh
AU  - Prokić, Marko
AU  - Petrović, Tamara
AU  - Jafari, Fateme
AU  - Moradian, Seyed Hossein
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6551
AB  - Dynamic changes of tissues, organs and growth that occur in fish larvae during the transition to the juvenile stage are accompanied by differences in metabolic, locomotor and feeding activities that can reflect on fish's oxidative status. In this study, we examine how body growth, antioxidant system (superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) and total antioxidant capacity (TAC) and oxidative damage (malondialdehyde-MDA) parameters change in larvae of yellowfin seabream larvae (Acanthopagrus latus) during early development (0, 7, 15, 22 and 30 day after hatching-DAH). Body growth (length and weight) starts to intensify from 15 DAH. We observed general increase in the antioxidant system (AOS) with the age of larvae from newly hatched and 7 DAH up to 15 and 22 DAH individuals. 15 and 22 DAH larvae had the greatest levels of TAC, SOD and GR activity, while 30 DAH larvae had higher CAT activity from 0, 7 and 15 DAH and MDA concentration in comparison to 15 DAH individuals. Several developmental events can be linked with observed results: lower AOS in 0 and 7 DAH individuals with low locomotor activity, growth, endo-exogenous feeding phase and cell differentiation; 15 and 22 DAH larvae are under pressure of fast growth, enhanced swimming and foraging capacity; while higher MDA production in 30 DAH larvae can be a result of shifts in muscle metabolism, changes in both quality and quantity of food and a significant increase in weight. The present study provides insight into the changes in redox status during the ontogeny of A. latus, fish species about which physiology is still little known but with a potential for use in marine culture. Ability to lower oxidative stress during critical developmental periods can enhance that potential.
PB  - Amsterdam, Netherlands: Elsevier
T2  - Comparative Biochemistry and Physiology, Part A: Molecular and Integrative Physiology
T1  - Changes of oxidative status in yellowfin seabream larvae (Acanthopagrus latus) during development
VL  - 281
DO  - 10.1016/j.cbpa.2023.111433
SP  - 111433
ER  - 

@article{
author = "Morshedi, Vahid and Noori, Farzaneh and Prokić, Marko and Petrović, Tamara and Jafari, Fateme and Moradian, Seyed Hossein",
year = "2023",
abstract = "Dynamic changes of tissues, organs and growth that occur in fish larvae during the transition to the juvenile stage are accompanied by differences in metabolic, locomotor and feeding activities that can reflect on fish's oxidative status. In this study, we examine how body growth, antioxidant system (superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) and total antioxidant capacity (TAC) and oxidative damage (malondialdehyde-MDA) parameters change in larvae of yellowfin seabream larvae (Acanthopagrus latus) during early development (0, 7, 15, 22 and 30 day after hatching-DAH). Body growth (length and weight) starts to intensify from 15 DAH. We observed general increase in the antioxidant system (AOS) with the age of larvae from newly hatched and 7 DAH up to 15 and 22 DAH individuals. 15 and 22 DAH larvae had the greatest levels of TAC, SOD and GR activity, while 30 DAH larvae had higher CAT activity from 0, 7 and 15 DAH and MDA concentration in comparison to 15 DAH individuals. Several developmental events can be linked with observed results: lower AOS in 0 and 7 DAH individuals with low locomotor activity, growth, endo-exogenous feeding phase and cell differentiation; 15 and 22 DAH larvae are under pressure of fast growth, enhanced swimming and foraging capacity; while higher MDA production in 30 DAH larvae can be a result of shifts in muscle metabolism, changes in both quality and quantity of food and a significant increase in weight. The present study provides insight into the changes in redox status during the ontogeny of A. latus, fish species about which physiology is still little known but with a potential for use in marine culture. Ability to lower oxidative stress during critical developmental periods can enhance that potential.",
publisher = "Amsterdam, Netherlands: Elsevier",
journal = "Comparative Biochemistry and Physiology, Part A: Molecular and Integrative Physiology",
title = "Changes of oxidative status in yellowfin seabream larvae (Acanthopagrus latus) during development",
volume = "281",
doi = "10.1016/j.cbpa.2023.111433",
pages = "111433"
}

Morshedi, V., Noori, F., Prokić, M., Petrović, T., Jafari, F.,& Moradian, S. H.. (2023). Changes of oxidative status in yellowfin seabream larvae (Acanthopagrus latus) during development. in Comparative Biochemistry and Physiology, Part A: Molecular and Integrative Physiology
Amsterdam, Netherlands: Elsevier., 281, 111433.
https://doi.org/10.1016/j.cbpa.2023.111433

Morshedi V, Noori F, Prokić M, Petrović T, Jafari F, Moradian SH. Changes of oxidative status in yellowfin seabream larvae (Acanthopagrus latus) during development. in Comparative Biochemistry and Physiology, Part A: Molecular and Integrative Physiology. 2023;281:111433.
doi:10.1016/j.cbpa.2023.111433 .

Morshedi, Vahid, Noori, Farzaneh, Prokić, Marko, Petrović, Tamara, Jafari, Fateme, Moradian, Seyed Hossein, "Changes of oxidative status in yellowfin seabream larvae (Acanthopagrus latus) during development" in Comparative Biochemistry and Physiology, Part A: Molecular and Integrative Physiology, 281 (2023):111433,
https://doi.org/10.1016/j.cbpa.2023.111433 . .

TY  - JOUR
AU  - Morshedi, Vahid
AU  - Noori, Farzaneh
AU  - Prokić, Marko
AU  - Petrović, Tamara
AU  - Jafari, Fateme
AU  - Moradian, Seyed Hossein
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6549
AB  - Dynamic changes of tissues, organs and growth that occur in fish larvae during the transition to the juvenile stage are accompanied by differences in metabolic, locomotor and feeding activities that can reflect on fish's oxidative status. In this study, we examine how body growth, antioxidant system (superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) and total antioxidant capacity (TAC) and oxidative damage (malondialdehyde-MDA) parameters change in larvae of yellowfin seabream larvae (Acanthopagrus latus) during early development (0, 7, 15, 22 and 30 day after hatching-DAH). Body growth (length and weight) starts to intensify from 15 DAH. We observed general increase in the antioxidant system (AOS) with the age of larvae from newly hatched and 7 DAH up to 15 and 22 DAH individuals. 15 and 22 DAH larvae had the greatest levels of TAC, SOD and GR activity, while 30 DAH larvae had higher CAT activity from 0, 7 and 15 DAH and MDA concentration in comparison to 15 DAH individuals. Several developmental events can be linked with observed results: lower AOS in 0 and 7 DAH individuals with low locomotor activity, growth, endo-exogenous feeding phase and cell differentiation; 15 and 22 DAH larvae are under pressure of fast growth, enhanced swimming and foraging capacity; while higher MDA production in 30 DAH larvae can be a result of shifts in muscle metabolism, changes in both quality and quantity of food and a significant increase in weight. The present study provides insight into the changes in redox status during the ontogeny of A. latus, fish species about which physiology is still little known but with a potential for use in marine culture. Ability to lower oxidative stress during critical developmental periods can enhance that potential.
PB  - Amsterdam, Netherlands: Elsevier
T2  - Comparative Biochemistry and Physiology, Part A: Molecular and Integrative Physiology
T1  - Changes of oxidative status in yellowfin seabream larvae (Acanthopagrus latus) during development
VL  - 281
DO  - 10.1016/j.cbpa.2023.111433
SP  - 111433
ER  - 

@article{
author = "Morshedi, Vahid and Noori, Farzaneh and Prokić, Marko and Petrović, Tamara and Jafari, Fateme and Moradian, Seyed Hossein",
year = "2023",
abstract = "Dynamic changes of tissues, organs and growth that occur in fish larvae during the transition to the juvenile stage are accompanied by differences in metabolic, locomotor and feeding activities that can reflect on fish's oxidative status. In this study, we examine how body growth, antioxidant system (superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) and total antioxidant capacity (TAC) and oxidative damage (malondialdehyde-MDA) parameters change in larvae of yellowfin seabream larvae (Acanthopagrus latus) during early development (0, 7, 15, 22 and 30 day after hatching-DAH). Body growth (length and weight) starts to intensify from 15 DAH. We observed general increase in the antioxidant system (AOS) with the age of larvae from newly hatched and 7 DAH up to 15 and 22 DAH individuals. 15 and 22 DAH larvae had the greatest levels of TAC, SOD and GR activity, while 30 DAH larvae had higher CAT activity from 0, 7 and 15 DAH and MDA concentration in comparison to 15 DAH individuals. Several developmental events can be linked with observed results: lower AOS in 0 and 7 DAH individuals with low locomotor activity, growth, endo-exogenous feeding phase and cell differentiation; 15 and 22 DAH larvae are under pressure of fast growth, enhanced swimming and foraging capacity; while higher MDA production in 30 DAH larvae can be a result of shifts in muscle metabolism, changes in both quality and quantity of food and a significant increase in weight. The present study provides insight into the changes in redox status during the ontogeny of A. latus, fish species about which physiology is still little known but with a potential for use in marine culture. Ability to lower oxidative stress during critical developmental periods can enhance that potential.",
publisher = "Amsterdam, Netherlands: Elsevier",
journal = "Comparative Biochemistry and Physiology, Part A: Molecular and Integrative Physiology",
title = "Changes of oxidative status in yellowfin seabream larvae (Acanthopagrus latus) during development",
volume = "281",
doi = "10.1016/j.cbpa.2023.111433",
pages = "111433"
}

Morshedi, V., Noori, F., Prokić, M., Petrović, T., Jafari, F.,& Moradian, S. H.. (2023). Changes of oxidative status in yellowfin seabream larvae (Acanthopagrus latus) during development. in Comparative Biochemistry and Physiology, Part A: Molecular and Integrative Physiology
Amsterdam, Netherlands: Elsevier., 281, 111433.
https://doi.org/10.1016/j.cbpa.2023.111433

Morshedi V, Noori F, Prokić M, Petrović T, Jafari F, Moradian SH. Changes of oxidative status in yellowfin seabream larvae (Acanthopagrus latus) during development. in Comparative Biochemistry and Physiology, Part A: Molecular and Integrative Physiology. 2023;281:111433.
doi:10.1016/j.cbpa.2023.111433 .

Morshedi, Vahid, Noori, Farzaneh, Prokić, Marko, Petrović, Tamara, Jafari, Fateme, Moradian, Seyed Hossein, "Changes of oxidative status in yellowfin seabream larvae (Acanthopagrus latus) during development" in Comparative Biochemistry and Physiology, Part A: Molecular and Integrative Physiology, 281 (2023):111433,
https://doi.org/10.1016/j.cbpa.2023.111433 . .

TY  - CONF
AU  - Kijanović, Ana
AU  - Vukov, Tanja
AU  - Mirč, Marko
AU  - Mitrović, Aleksandar
AU  - Prokić, Marko
AU  - Petrović, Tamara
AU  - Radovanović, Tijana
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Tomašević Kolarov, Nataša
PY  - 2023
UR  - https://www.sebiology.org/events/seb-centenary-conference-2023/abstracts.html
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6060
AB  - Amphibian species that inhabit temporary ponds for reproduction maximize larval growth under favorable conditions and accelerate their development to undergo rapid metamorphosis under stressful conditions such as pond drying. Corticosterone controls development, metabolism, and growth, and has an invaluable role in anuran metamorphosis under stress conditions. In this study, we evaluated whether the whole-body corticosterone (CORT) level is related to drying conditions in species that cannot accelerate the developmental rate in response to pond drying. Specifically, we investigated the effects of different water levels in combination with exogenous CORT and corticosteroid synthesis inhibitor metyrapone (treatments: high water level, high water level with exogenous CORT, low water level, low water level with metyrapone) on the whole-body corticosterone (at prometamorphosis and metamorphic climax), life history and morphological traits at the metamorphic climax. We found that these conditions did not alter the whole-body content of CORT and the developmental rate in treatments, although low water levels and exogenous CORT in high water level negatively affected other life history traits and tail shape. Individuals from a high water level with exogenous CORT had the smallest body size and mass and changed tail shape at metamorphosis, while changes in life history traits did not affect the tail shape in the other treatments. Our findings indicate that the absence of developmental response (i.e. canalized development) in the timing of metamorphosis of B. variegata may be explained by a modification of endocrine regulation but further studies that would include closely related species are required.
PB  - Society for Experimental Biology
C3  - Abstract book: SEB Centenary Conference 1923-2023: Celebrating Success and Shaping the Future; 2023 Jul 4-7; Edinburgh, UK
T1  - Pond drying conditions do not alter whole-body corticosterone content and metamorphic time of yellow-bellied toad (Bombina variegata) metamorphs
SP  - 8
EP  - 9
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6060
ER  - 

@conference{
author = "Kijanović, Ana and Vukov, Tanja and Mirč, Marko and Mitrović, Aleksandar and Prokić, Marko and Petrović, Tamara and Radovanović, Tijana and Gavrilović, Branka and Despotović, Svetlana and Gavrić, Jelena and Tomašević Kolarov, Nataša",
year = "2023",
abstract = "Amphibian species that inhabit temporary ponds for reproduction maximize larval growth under favorable conditions and accelerate their development to undergo rapid metamorphosis under stressful conditions such as pond drying. Corticosterone controls development, metabolism, and growth, and has an invaluable role in anuran metamorphosis under stress conditions. In this study, we evaluated whether the whole-body corticosterone (CORT) level is related to drying conditions in species that cannot accelerate the developmental rate in response to pond drying. Specifically, we investigated the effects of different water levels in combination with exogenous CORT and corticosteroid synthesis inhibitor metyrapone (treatments: high water level, high water level with exogenous CORT, low water level, low water level with metyrapone) on the whole-body corticosterone (at prometamorphosis and metamorphic climax), life history and morphological traits at the metamorphic climax. We found that these conditions did not alter the whole-body content of CORT and the developmental rate in treatments, although low water levels and exogenous CORT in high water level negatively affected other life history traits and tail shape. Individuals from a high water level with exogenous CORT had the smallest body size and mass and changed tail shape at metamorphosis, while changes in life history traits did not affect the tail shape in the other treatments. Our findings indicate that the absence of developmental response (i.e. canalized development) in the timing of metamorphosis of B. variegata may be explained by a modification of endocrine regulation but further studies that would include closely related species are required.",
publisher = "Society for Experimental Biology",
journal = "Abstract book: SEB Centenary Conference 1923-2023: Celebrating Success and Shaping the Future; 2023 Jul 4-7; Edinburgh, UK",
title = "Pond drying conditions do not alter whole-body corticosterone content and metamorphic time of yellow-bellied toad (Bombina variegata) metamorphs",
pages = "8-9",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6060"
}

Kijanović, A., Vukov, T., Mirč, M., Mitrović, A., Prokić, M., Petrović, T., Radovanović, T., Gavrilović, B., Despotović, S., Gavrić, J.,& Tomašević Kolarov, N.. (2023). Pond drying conditions do not alter whole-body corticosterone content and metamorphic time of yellow-bellied toad (Bombina variegata) metamorphs. in Abstract book: SEB Centenary Conference 1923-2023: Celebrating Success and Shaping the Future; 2023 Jul 4-7; Edinburgh, UK
Society for Experimental Biology., 8-9.
https://hdl.handle.net/21.15107/rcub_ibiss_6060

Kijanović A, Vukov T, Mirč M, Mitrović A, Prokić M, Petrović T, Radovanović T, Gavrilović B, Despotović S, Gavrić J, Tomašević Kolarov N. Pond drying conditions do not alter whole-body corticosterone content and metamorphic time of yellow-bellied toad (Bombina variegata) metamorphs. in Abstract book: SEB Centenary Conference 1923-2023: Celebrating Success and Shaping the Future; 2023 Jul 4-7; Edinburgh, UK. 2023;:8-9.
https://hdl.handle.net/21.15107/rcub_ibiss_6060 .

Kijanović, Ana, Vukov, Tanja, Mirč, Marko, Mitrović, Aleksandar, Prokić, Marko, Petrović, Tamara, Radovanović, Tijana, Gavrilović, Branka, Despotović, Svetlana, Gavrić, Jelena, Tomašević Kolarov, Nataša, "Pond drying conditions do not alter whole-body corticosterone content and metamorphic time of yellow-bellied toad (Bombina variegata) metamorphs" in Abstract book: SEB Centenary Conference 1923-2023: Celebrating Success and Shaping the Future; 2023 Jul 4-7; Edinburgh, UK (2023):8-9,
https://hdl.handle.net/21.15107/rcub_ibiss_6060 .

TY  - JOUR
AU  - Petrović, Tamara
AU  - Vučić, Tijana
AU  - Burraco, Pablo
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Radovanović, Tijana
AU  - Šajkunić, Sanja
AU  - Ivanović, Ana
AU  - Prokić, Marko
PY  - 2023
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0306456523000153
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5463
AB  - Ectotherms are particularly sensitive to global warming due to their limited capacity to thermoregulate, which can impact their performance and fitness. From a physiological standpoint, higher temperatures often enhance biological processes that can induce the production of reactive oxygen species and result in a state of cellular oxidative stress. Temperature alters interspecific interactions, including species hybridization. Hybridization under different thermal conditions could amplify parental (genetic) incompatibilities, thus affecting a hybrid's development and distribution. Understanding the impact of global warming on the physiology of hybrids and particularly their oxidative status could help in predicting future scenarios in ecosystems and in hybrids. In the present study, we investigated the effect of water temperature on the development, growth and oxidative stress of two crested newt species and their reciprocal hybrids. Larvae of Triturus macedonicus and T. ivanbureschi, and their T. macedonicus-mothered and T. ivanbureschi-mothered hybrids were exposed for 30 days to temperatures of 19°C and 24°C. Under the higher temperature, the hybrids experienced increases in both growth and developmental rates, while parental species exhibited accelerated growth (T. macedonicus) or development (T. ivanbureschi). Warm conditions also had different effects on the oxidative status of hybrid and parental species. Parental species had enhanced antioxidant responses (catalase, glutathione peroxidase, glutathione S-transferase and SH groups), which allowed them to alleviate temperature-induced stress (revealed by the absence of oxidative damage). However, warming induced an antioxidant response in the hybrids, including oxidative damage in the form of lipid peroxidation. These findings point to a greater disruption of redox regulation and metabolic machinery in hybrid newts, which can be interpreted as the cost of hybridization that is likely linked to parental incompatibilities expressed under a higher temperature. Our study aims to improve mechanistic understanding of the resilience and distribution of hybrid species that cope with climate-driven changes.
PB  - Elsevier Ltd
T2  - Journal of Thermal Biology
T1  - Higher temperature induces oxidative stress in hybrids but not in parental species: A case study of crested newts
VL  - 112
DO  - 10.1016/j.jtherbio.2023.103474
SP  - 103474
ER  - 

@article{
author = "Petrović, Tamara and Vučić, Tijana and Burraco, Pablo and Gavrilović, Branka and Despotović, Svetlana and Gavrić, Jelena and Radovanović, Tijana and Šajkunić, Sanja and Ivanović, Ana and Prokić, Marko",
year = "2023",
abstract = "Ectotherms are particularly sensitive to global warming due to their limited capacity to thermoregulate, which can impact their performance and fitness. From a physiological standpoint, higher temperatures often enhance biological processes that can induce the production of reactive oxygen species and result in a state of cellular oxidative stress. Temperature alters interspecific interactions, including species hybridization. Hybridization under different thermal conditions could amplify parental (genetic) incompatibilities, thus affecting a hybrid's development and distribution. Understanding the impact of global warming on the physiology of hybrids and particularly their oxidative status could help in predicting future scenarios in ecosystems and in hybrids. In the present study, we investigated the effect of water temperature on the development, growth and oxidative stress of two crested newt species and their reciprocal hybrids. Larvae of Triturus macedonicus and T. ivanbureschi, and their T. macedonicus-mothered and T. ivanbureschi-mothered hybrids were exposed for 30 days to temperatures of 19°C and 24°C. Under the higher temperature, the hybrids experienced increases in both growth and developmental rates, while parental species exhibited accelerated growth (T. macedonicus) or development (T. ivanbureschi). Warm conditions also had different effects on the oxidative status of hybrid and parental species. Parental species had enhanced antioxidant responses (catalase, glutathione peroxidase, glutathione S-transferase and SH groups), which allowed them to alleviate temperature-induced stress (revealed by the absence of oxidative damage). However, warming induced an antioxidant response in the hybrids, including oxidative damage in the form of lipid peroxidation. These findings point to a greater disruption of redox regulation and metabolic machinery in hybrid newts, which can be interpreted as the cost of hybridization that is likely linked to parental incompatibilities expressed under a higher temperature. Our study aims to improve mechanistic understanding of the resilience and distribution of hybrid species that cope with climate-driven changes.",
publisher = "Elsevier Ltd",
journal = "Journal of Thermal Biology",
title = "Higher temperature induces oxidative stress in hybrids but not in parental species: A case study of crested newts",
volume = "112",
doi = "10.1016/j.jtherbio.2023.103474",
pages = "103474"
}

Petrović, T., Vučić, T., Burraco, P., Gavrilović, B., Despotović, S., Gavrić, J., Radovanović, T., Šajkunić, S., Ivanović, A.,& Prokić, M.. (2023). Higher temperature induces oxidative stress in hybrids but not in parental species: A case study of crested newts. in Journal of Thermal Biology
Elsevier Ltd., 112, 103474.
https://doi.org/10.1016/j.jtherbio.2023.103474

Petrović T, Vučić T, Burraco P, Gavrilović B, Despotović S, Gavrić J, Radovanović T, Šajkunić S, Ivanović A, Prokić M. Higher temperature induces oxidative stress in hybrids but not in parental species: A case study of crested newts. in Journal of Thermal Biology. 2023;112:103474.
doi:10.1016/j.jtherbio.2023.103474 .

Petrović, Tamara, Vučić, Tijana, Burraco, Pablo, Gavrilović, Branka, Despotović, Svetlana, Gavrić, Jelena, Radovanović, Tijana, Šajkunić, Sanja, Ivanović, Ana, Prokić, Marko, "Higher temperature induces oxidative stress in hybrids but not in parental species: A case study of crested newts" in Journal of Thermal Biology, 112 (2023):103474,
https://doi.org/10.1016/j.jtherbio.2023.103474 . .

TY  - JOUR
AU  - Radovanović, Tijana
AU  - Petrović, Tamara
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Kijanović, Ana
AU  - Mirč, Marko
AU  - Tomašević Kolarov, Nataša
AU  - Vukov, Tanja
AU  - Prokić, Marko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5464
AB  - Background Urban development results in habitat destruction, affecting populations of amphibians, the most fragile
group of vertebrates. With changes in the environment, these animals become more exposed to light and predators.
To enhance their chances of survival, they display plasticity of body coloration. Aside from adaptive benefits,
animals exhibiting background matching meet the energetic costs and restrictions of changing body tones. To study
the physiological consequences of Hyla arborea tadpole adaptation to background color, we followed oxidative stress
parameters after rearing larvae on a constant background (black/white) and after changing the background color.
Results Larvae cultivated for 20 days on constant substrate color exhibited differences in body coloration but without
differences in lipid peroxidation (LPO) concentration between dark and pale individuals, suggesting that coloration
investment during this period did not induce higher oxidative damage in darker tadpoles. Prolonged exposure
of larvae (37 days) to a dark habitat increased antioxidative system defense and LPO concentrations, compared to
animals reared permanently in the white surroundings. The positive correlation of oxidative damage with color
intensity of individuals points to the physiological consequences of higher investment in the number of pigment cells
necessary for dark pigmentation. In individuals faced with non-matching background and change in body coloration,
defense system declined and LPO occurred relative to individuals cultivated in white habitat.
Conclusion Here, we have pointed to consequences related to background matching and stress that amphibians
experienced during chromatic adaptations. Background color change causes a complex physiological response
affecting the antioxidative defense parameters. This investigation elucidates the accompanying cost of amphibiansʼ
adjustment to an altered environment.
PB  - London: BMC
T2  - Frontiers in Zoology
T1  - What coloration brings: Implications of background adaptation to oxidative stress in anurans
IS  - 1
VL  - 20
DO  - 10.1186/s12983-023-00486-z
SP  - 6
ER  - 

@article{
author = "Radovanović, Tijana and Petrović, Tamara and Gavrilović, Branka and Despotović, Svetlana and Gavrić, Jelena and Kijanović, Ana and Mirč, Marko and Tomašević Kolarov, Nataša and Vukov, Tanja and Prokić, Marko",
year = "2023",
abstract = "Background Urban development results in habitat destruction, affecting populations of amphibians, the most fragile
group of vertebrates. With changes in the environment, these animals become more exposed to light and predators.
To enhance their chances of survival, they display plasticity of body coloration. Aside from adaptive benefits,
animals exhibiting background matching meet the energetic costs and restrictions of changing body tones. To study
the physiological consequences of Hyla arborea tadpole adaptation to background color, we followed oxidative stress
parameters after rearing larvae on a constant background (black/white) and after changing the background color.
Results Larvae cultivated for 20 days on constant substrate color exhibited differences in body coloration but without
differences in lipid peroxidation (LPO) concentration between dark and pale individuals, suggesting that coloration
investment during this period did not induce higher oxidative damage in darker tadpoles. Prolonged exposure
of larvae (37 days) to a dark habitat increased antioxidative system defense and LPO concentrations, compared to
animals reared permanently in the white surroundings. The positive correlation of oxidative damage with color
intensity of individuals points to the physiological consequences of higher investment in the number of pigment cells
necessary for dark pigmentation. In individuals faced with non-matching background and change in body coloration,
defense system declined and LPO occurred relative to individuals cultivated in white habitat.
Conclusion Here, we have pointed to consequences related to background matching and stress that amphibians
experienced during chromatic adaptations. Background color change causes a complex physiological response
affecting the antioxidative defense parameters. This investigation elucidates the accompanying cost of amphibiansʼ
adjustment to an altered environment.",
publisher = "London: BMC",
journal = "Frontiers in Zoology",
title = "What coloration brings: Implications of background adaptation to oxidative stress in anurans",
number = "1",
volume = "20",
doi = "10.1186/s12983-023-00486-z",
pages = "6"
}

Radovanović, T., Petrović, T., Gavrilović, B., Despotović, S., Gavrić, J., Kijanović, A., Mirč, M., Tomašević Kolarov, N., Vukov, T.,& Prokić, M.. (2023). What coloration brings: Implications of background adaptation to oxidative stress in anurans. in Frontiers in Zoology
London: BMC., 20(1), 6.
https://doi.org/10.1186/s12983-023-00486-z

Radovanović T, Petrović T, Gavrilović B, Despotović S, Gavrić J, Kijanović A, Mirč M, Tomašević Kolarov N, Vukov T, Prokić M. What coloration brings: Implications of background adaptation to oxidative stress in anurans. in Frontiers in Zoology. 2023;20(1):6.
doi:10.1186/s12983-023-00486-z .

Radovanović, Tijana, Petrović, Tamara, Gavrilović, Branka, Despotović, Svetlana, Gavrić, Jelena, Kijanović, Ana, Mirč, Marko, Tomašević Kolarov, Nataša, Vukov, Tanja, Prokić, Marko, "What coloration brings: Implications of background adaptation to oxidative stress in anurans" in Frontiers in Zoology, 20, no. 1 (2023):6,
https://doi.org/10.1186/s12983-023-00486-z . .

TY  - CONF
AU  - Kijanović, Ana
AU  - Vukov, Tanja
AU  - Mirč, Marko
AU  - Mitrović, Aleksandar
AU  - Prokić, Marko
AU  - Radovanović, Tijana
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Petrović, Tamara
AU  - Tomašević Kolarov, Nataša
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5250
AB  - Many amphibian species inhabit temporary aquatic habitats for reproduction to
maximize larval growth under favorable conditions but accelerate developmental rate
to rapidly undergo metamorphosis under stress conditions when high desiccation risk
exists. Corticosterone, one of the most important vertebrate stress hormone controls
development, metabolism, and skeletal growth and together with thyroid hormone
have a critical role in anuran metamorphosis under stress conditions. Here we
compare the hormonal variation of corticosterone (CORT) in the yellow-bellied toad
Bombina variegata tadpoles in response to different water levels (constant highcontrol, constant low, constant high+exogenous CORT) and the corticosterone
inhibitor- metyrapone (MET) (treatment constant low water level+MET). Also, we
investigated the effects of CORT and MET on life-history and morphological traits at
metamorphosis. We found that B. variegata tadpoles cannot accelerate the
developmental rate in response to pond drying. The constant low water level did not
alter whole-body content of CORT and it did not significantly differ from high water
level treatment with exogenous CORT. However, individuals from high water level with
exogenous CORT have prolonged development, the highest mortality rate, the smallest
body size and mass, and the widest but the shortest tails at metamorphosis. Constant
low water level with MET decreased the whole-body content of CORT and it was
significantly different between all groups, but it did not affect the developmental rate
and tail shape although these tadpoles have intermediate size and mass between high
water levels with exogenous CORT and control. We show that responsiveness to pond
drying of Bombina variegata and its short larval period is probably associated with
modified endocrine signaling pathway that control tadpole metamorphosis.
Constitutively high whole-body content of CORT which was unaltered by pond drying
leads us to conclusion that Bombina variegata development may be canalized but more
research is needed.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
T1  - Desiccation stress response of Bombina variegata tadpoles
SP  - 158
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5250
ER  - 

@conference{
author = "Kijanović, Ana and Vukov, Tanja and Mirč, Marko and Mitrović, Aleksandar and Prokić, Marko and Radovanović, Tijana and Gavrilović, Branka and Despotović, Svetlana and Gavrić, Jelena and Petrović, Tamara and Tomašević Kolarov, Nataša",
year = "2022",
abstract = "Many amphibian species inhabit temporary aquatic habitats for reproduction to
maximize larval growth under favorable conditions but accelerate developmental rate
to rapidly undergo metamorphosis under stress conditions when high desiccation risk
exists. Corticosterone, one of the most important vertebrate stress hormone controls
development, metabolism, and skeletal growth and together with thyroid hormone
have a critical role in anuran metamorphosis under stress conditions. Here we
compare the hormonal variation of corticosterone (CORT) in the yellow-bellied toad
Bombina variegata tadpoles in response to different water levels (constant highcontrol, constant low, constant high+exogenous CORT) and the corticosterone
inhibitor- metyrapone (MET) (treatment constant low water level+MET). Also, we
investigated the effects of CORT and MET on life-history and morphological traits at
metamorphosis. We found that B. variegata tadpoles cannot accelerate the
developmental rate in response to pond drying. The constant low water level did not
alter whole-body content of CORT and it did not significantly differ from high water
level treatment with exogenous CORT. However, individuals from high water level with
exogenous CORT have prolonged development, the highest mortality rate, the smallest
body size and mass, and the widest but the shortest tails at metamorphosis. Constant
low water level with MET decreased the whole-body content of CORT and it was
significantly different between all groups, but it did not affect the developmental rate
and tail shape although these tadpoles have intermediate size and mass between high
water levels with exogenous CORT and control. We show that responsiveness to pond
drying of Bombina variegata and its short larval period is probably associated with
modified endocrine signaling pathway that control tadpole metamorphosis.
Constitutively high whole-body content of CORT which was unaltered by pond drying
leads us to conclusion that Bombina variegata development may be canalized but more
research is needed.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia",
title = "Desiccation stress response of Bombina variegata tadpoles",
pages = "158",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5250"
}

Kijanović, A., Vukov, T., Mirč, M., Mitrović, A., Prokić, M., Radovanović, T., Gavrilović, B., Despotović, S., Gavrić, J., Petrović, T.,& Tomašević Kolarov, N.. (2022). Desiccation stress response of Bombina variegata tadpoles. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 158.
https://hdl.handle.net/21.15107/rcub_ibiss_5250

Kijanović A, Vukov T, Mirč M, Mitrović A, Prokić M, Radovanović T, Gavrilović B, Despotović S, Gavrić J, Petrović T, Tomašević Kolarov N. Desiccation stress response of Bombina variegata tadpoles. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. 2022;:158.
https://hdl.handle.net/21.15107/rcub_ibiss_5250 .

Kijanović, Ana, Vukov, Tanja, Mirč, Marko, Mitrović, Aleksandar, Prokić, Marko, Radovanović, Tijana, Gavrilović, Branka, Despotović, Svetlana, Gavrić, Jelena, Petrović, Tamara, Tomašević Kolarov, Nataša, "Desiccation stress response of Bombina variegata tadpoles" in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia (2022):158,
https://hdl.handle.net/21.15107/rcub_ibiss_5250 .

TY  - JOUR
AU  - Petrović, Tamara
AU  - Vukov, Tanja
AU  - Tomašević Kolarov, Nataša
PY  - 2021
UR  - http://www.ncbi.nlm.nih.gov/pubmed/33220626
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0944200620301239
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4012
AB  - As anuran saltatory locomotion has specific functional requirements achieved through certain intra- and inter-limb proportions, we analyzed pattern and degree of morphological integration in limbs of ten anuran species to reveal the relationship of shared developmental programs of serially homologous structures and locomotor specialization. Our main objectives were (1) to examine if morphological and functional differences in forelimb and hindlimb were associated with reduced covariation between limbs, (2) and to reveal patterns of correlation between species and the roles played by evolutionary history (phylogeny) and ecology (lifestyle and habitat use). Species with different locomotor behaviours (walking, jumping, hopping, running, climbing, swimming and burrowing) were used. Partial correlations showed that species shared similar patterns of functionally based morphological integration, with increased correlations in elements within limbs and reduced correlations between limbs. This was mainly based on strong correlations between proximal elements, humerus-radioulna and femur-tibiofibula. To test the influence of phylogenetic relationships and ecological demands we used different matrices (correlation similarity matrix, ecological similarity matrix, matrices of phylogenetic distance and morphological distance). The changes in correlation patterns are shown to be dissociated from phylogeny. On the other hand, they are to some extent shaped by habitat use and locomotion, as the species with similar locomotor behaviour also tend to have stronger similarity in integration patterns. The results from this study provide insight into the processes underlying the evolutionary change of anuran limbs, highlighting function as the main factor that shaped morphological integration of the examined species.
PB  - Elsevier BV
T2  - Zoology
T1  - Patterns of correlations and locomotor specialization in anuran limbs: association with phylogeny and ecology
VL  - 144
DO  - 10.1016/j.zool.2020.125864
SP  - 125864
ER  - 

@article{
author = "Petrović, Tamara and Vukov, Tanja and Tomašević Kolarov, Nataša",
year = "2021",
abstract = "As anuran saltatory locomotion has specific functional requirements achieved through certain intra- and inter-limb proportions, we analyzed pattern and degree of morphological integration in limbs of ten anuran species to reveal the relationship of shared developmental programs of serially homologous structures and locomotor specialization. Our main objectives were (1) to examine if morphological and functional differences in forelimb and hindlimb were associated with reduced covariation between limbs, (2) and to reveal patterns of correlation between species and the roles played by evolutionary history (phylogeny) and ecology (lifestyle and habitat use). Species with different locomotor behaviours (walking, jumping, hopping, running, climbing, swimming and burrowing) were used. Partial correlations showed that species shared similar patterns of functionally based morphological integration, with increased correlations in elements within limbs and reduced correlations between limbs. This was mainly based on strong correlations between proximal elements, humerus-radioulna and femur-tibiofibula. To test the influence of phylogenetic relationships and ecological demands we used different matrices (correlation similarity matrix, ecological similarity matrix, matrices of phylogenetic distance and morphological distance). The changes in correlation patterns are shown to be dissociated from phylogeny. On the other hand, they are to some extent shaped by habitat use and locomotion, as the species with similar locomotor behaviour also tend to have stronger similarity in integration patterns. The results from this study provide insight into the processes underlying the evolutionary change of anuran limbs, highlighting function as the main factor that shaped morphological integration of the examined species.",
publisher = "Elsevier BV",
journal = "Zoology",
title = "Patterns of correlations and locomotor specialization in anuran limbs: association with phylogeny and ecology",
volume = "144",
doi = "10.1016/j.zool.2020.125864",
pages = "125864"
}

Petrović, T., Vukov, T.,& Tomašević Kolarov, N.. (2021). Patterns of correlations and locomotor specialization in anuran limbs: association with phylogeny and ecology. in Zoology
Elsevier BV., 144, 125864.
https://doi.org/10.1016/j.zool.2020.125864

Petrović T, Vukov T, Tomašević Kolarov N. Patterns of correlations and locomotor specialization in anuran limbs: association with phylogeny and ecology. in Zoology. 2021;144:125864.
doi:10.1016/j.zool.2020.125864 .

Petrović, Tamara, Vukov, Tanja, Tomašević Kolarov, Nataša, "Patterns of correlations and locomotor specialization in anuran limbs: association with phylogeny and ecology" in Zoology, 144 (2021):125864,
https://doi.org/10.1016/j.zool.2020.125864 . .

TY  - JOUR
AU  - Prokić, Marko
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Gavrić, Jelena
AU  - Petrović, Tamara
AU  - Despotović, Svetlana
AU  - Faggio, Caterina
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4164
AB  - Although we are witnesses of an increase in the number of studies examining the exposure/effects of microplastics (MPs) on different organisms, there are many unknowns. This review aims to: (i) analyze current studies devoted to investigating the exposure/effects of MPs on animals; (ii) provide some basic knowledge about different model organisms and experimental approaches used in studying MPs; and to (iii) convey directions for future studies. We have summarized data from 500 studies published from January 2011 to May 2020, about different aspects of model organisms (taxonomic group of organisms, type of ecosystem they inhabit, life-stage, sex, tissue and/or organ) and experimental design (laboratory/field, ingestion/bioaccumulation/effect). We also discuss and try to encourage investigation of some less studied organisms (terrestrial and freshwater species, among groups including Annelida, Nematoda, Echinodermata, Cnidaria, Rotifera, birds, amphibians, reptiles), and aspects of MP pollution (long-term field studies, comparative studies examining life stages, sexes, laboratory and field work). We hope that the information presented in this review will serve as a good starting point and will provide useful guidelines for researchers during the process of deciding on the model organism and study designs for investigating MPs.
PB  - Elsevier B.V.
T2  - Journal of Hazardous Materials
T1  - Studying microplastics: Lessons from evaluated literature on animal model organisms and experimental approaches
VL  - 414
DO  - 10.1016/j.jhazmat.2021.125476
SP  - 125476
ER  - 

@article{
author = "Prokić, Marko and Gavrilović, Branka and Radovanović, Tijana and Gavrić, Jelena and Petrović, Tamara and Despotović, Svetlana and Faggio, Caterina",
year = "2021",
abstract = "Although we are witnesses of an increase in the number of studies examining the exposure/effects of microplastics (MPs) on different organisms, there are many unknowns. This review aims to: (i) analyze current studies devoted to investigating the exposure/effects of MPs on animals; (ii) provide some basic knowledge about different model organisms and experimental approaches used in studying MPs; and to (iii) convey directions for future studies. We have summarized data from 500 studies published from January 2011 to May 2020, about different aspects of model organisms (taxonomic group of organisms, type of ecosystem they inhabit, life-stage, sex, tissue and/or organ) and experimental design (laboratory/field, ingestion/bioaccumulation/effect). We also discuss and try to encourage investigation of some less studied organisms (terrestrial and freshwater species, among groups including Annelida, Nematoda, Echinodermata, Cnidaria, Rotifera, birds, amphibians, reptiles), and aspects of MP pollution (long-term field studies, comparative studies examining life stages, sexes, laboratory and field work). We hope that the information presented in this review will serve as a good starting point and will provide useful guidelines for researchers during the process of deciding on the model organism and study designs for investigating MPs.",
publisher = "Elsevier B.V.",
journal = "Journal of Hazardous Materials",
title = "Studying microplastics: Lessons from evaluated literature on animal model organisms and experimental approaches",
volume = "414",
doi = "10.1016/j.jhazmat.2021.125476",
pages = "125476"
}

Prokić, M., Gavrilović, B., Radovanović, T., Gavrić, J., Petrović, T., Despotović, S.,& Faggio, C.. (2021). Studying microplastics: Lessons from evaluated literature on animal model organisms and experimental approaches. in Journal of Hazardous Materials
Elsevier B.V.., 414, 125476.
https://doi.org/10.1016/j.jhazmat.2021.125476

Prokić M, Gavrilović B, Radovanović T, Gavrić J, Petrović T, Despotović S, Faggio C. Studying microplastics: Lessons from evaluated literature on animal model organisms and experimental approaches. in Journal of Hazardous Materials. 2021;414:125476.
doi:10.1016/j.jhazmat.2021.125476 .

Prokić, Marko, Gavrilović, Branka, Radovanović, Tijana, Gavrić, Jelena, Petrović, Tamara, Despotović, Svetlana, Faggio, Caterina, "Studying microplastics: Lessons from evaluated literature on animal model organisms and experimental approaches" in Journal of Hazardous Materials, 414 (2021):125476,
https://doi.org/10.1016/j.jhazmat.2021.125476 . .

TY  - JOUR
AU  - Prokić, Marko
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Gavrić, Jelena
AU  - Petrović, Tamara
AU  - Despotović, Svetlana
AU  - Faggio, Caterina
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4164
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4165
AB  - Although we are witnesses of an increase in the number of studies examining the exposure/effects of microplastics (MPs) on different organisms, there are many unknowns. This review aims to: (i) analyze current studies devoted to investigating the exposure/effects of MPs on animals; (ii) provide some basic knowledge about different model organisms and experimental approaches used in studying MPs; and to (iii) convey directions for future studies. We have summarized data from 500 studies published from January 2011 to May 2020, about different aspects of model organisms (taxonomic group of organisms, type of ecosystem they inhabit, life-stage, sex, tissue and/or organ) and experimental design (laboratory/field, ingestion/bioaccumulation/effect). We also discuss and try to encourage investigation of some less studied organisms (terrestrial and freshwater species, among groups including Annelida, Nematoda, Echinodermata, Cnidaria, Rotifera, birds, amphibians, reptiles), and aspects of MP pollution (long-term field studies, comparative studies examining life stages, sexes, laboratory and field work). We hope that the information presented in this review will serve as a good starting point and will provide useful guidelines for researchers during the process of deciding on the model organism and study designs for investigating MPs.
PB  - Elsevier B.V.
T2  - Journal of Hazardous Materials
T1  - Studying microplastics: Lessons from evaluated literature on animal model organisms and experimental approaches
VL  - 414
DO  - 10.1016/j.jhazmat.2021.125476
SP  - 125476
ER  - 

@article{
author = "Prokić, Marko and Gavrilović, Branka and Radovanović, Tijana and Gavrić, Jelena and Petrović, Tamara and Despotović, Svetlana and Faggio, Caterina",
year = "2021",
abstract = "Although we are witnesses of an increase in the number of studies examining the exposure/effects of microplastics (MPs) on different organisms, there are many unknowns. This review aims to: (i) analyze current studies devoted to investigating the exposure/effects of MPs on animals; (ii) provide some basic knowledge about different model organisms and experimental approaches used in studying MPs; and to (iii) convey directions for future studies. We have summarized data from 500 studies published from January 2011 to May 2020, about different aspects of model organisms (taxonomic group of organisms, type of ecosystem they inhabit, life-stage, sex, tissue and/or organ) and experimental design (laboratory/field, ingestion/bioaccumulation/effect). We also discuss and try to encourage investigation of some less studied organisms (terrestrial and freshwater species, among groups including Annelida, Nematoda, Echinodermata, Cnidaria, Rotifera, birds, amphibians, reptiles), and aspects of MP pollution (long-term field studies, comparative studies examining life stages, sexes, laboratory and field work). We hope that the information presented in this review will serve as a good starting point and will provide useful guidelines for researchers during the process of deciding on the model organism and study designs for investigating MPs.",
publisher = "Elsevier B.V.",
journal = "Journal of Hazardous Materials",
title = "Studying microplastics: Lessons from evaluated literature on animal model organisms and experimental approaches",
volume = "414",
doi = "10.1016/j.jhazmat.2021.125476",
pages = "125476"
}

Prokić, M., Gavrilović, B., Radovanović, T., Gavrić, J., Petrović, T., Despotović, S.,& Faggio, C.. (2021). Studying microplastics: Lessons from evaluated literature on animal model organisms and experimental approaches. in Journal of Hazardous Materials
Elsevier B.V.., 414, 125476.
https://doi.org/10.1016/j.jhazmat.2021.125476

Prokić M, Gavrilović B, Radovanović T, Gavrić J, Petrović T, Despotović S, Faggio C. Studying microplastics: Lessons from evaluated literature on animal model organisms and experimental approaches. in Journal of Hazardous Materials. 2021;414:125476.
doi:10.1016/j.jhazmat.2021.125476 .

Prokić, Marko, Gavrilović, Branka, Radovanović, Tijana, Gavrić, Jelena, Petrović, Tamara, Despotović, Svetlana, Faggio, Caterina, "Studying microplastics: Lessons from evaluated literature on animal model organisms and experimental approaches" in Journal of Hazardous Materials, 414 (2021):125476,
https://doi.org/10.1016/j.jhazmat.2021.125476 . .

TY  - JOUR
AU  - Petrović, Tamara
AU  - Kijanović, Ana
AU  - Tomašević Kolarov, Nataša
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Vukov, Tanja
AU  - Faggio, Caterina
AU  - Prokić, Marko
PY  - 2021
UR  - https://www.mdpi.com/2076-2615/11/4/953
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4192
AB  - In this paper, we examined how the oxidative status (antioxidant system and oxidative damage) of Bombina variegata larvae changed during the metamorphic climax (Gosner stages: 42—beginning, 44—middle and 46—end) and compared the patterns and levels of oxidative stress parameters between individuals developing under constant water availability (control) and those developing under decreasing water availability (desiccation group). Our results revealed that larvae developing under decreasing water availability exhibited increased oxidative damage in the middle and end stages. This was followed by lower levels of glutathione in stages 44 and 46, as well as lower values of catalase, glutathione peroxidase, glutathione S-transferase and sulfhydryl groups in stage 46 (all in relation to control animals). Comparison between stages 42, 44 and 46 within treatments showed that individuals in the last stage demonstrated the highest intensities of lipid oxidative damage in both the control and desiccation groups. As for the parameters of the antioxidant system, control individuals displayed greater variety in response to changes induced by metamorphic climax than individuals exposed to desiccation treatment. The overall decrease in water availability during development led to increased oxidative stress and modifications in the pattern of AOS response to changes induced by metamorphic climax in larvae of B. variegata.
PB  - MDPI AG
T2  - Animals
T1  - Effects of Desiccation on Metamorphic Climax in Bombina variegata: Changes in Levels and Patterns of Oxidative Stress Parameters
IS  - 4
VL  - 11
DO  - 10.3390/ani11040953
SP  - 953
ER  - 

@article{
author = "Petrović, Tamara and Kijanović, Ana and Tomašević Kolarov, Nataša and Gavrić, Jelena and Despotović, Svetlana and Gavrilović, Branka and Radovanović, Tijana and Vukov, Tanja and Faggio, Caterina and Prokić, Marko",
year = "2021",
abstract = "In this paper, we examined how the oxidative status (antioxidant system and oxidative damage) of Bombina variegata larvae changed during the metamorphic climax (Gosner stages: 42—beginning, 44—middle and 46—end) and compared the patterns and levels of oxidative stress parameters between individuals developing under constant water availability (control) and those developing under decreasing water availability (desiccation group). Our results revealed that larvae developing under decreasing water availability exhibited increased oxidative damage in the middle and end stages. This was followed by lower levels of glutathione in stages 44 and 46, as well as lower values of catalase, glutathione peroxidase, glutathione S-transferase and sulfhydryl groups in stage 46 (all in relation to control animals). Comparison between stages 42, 44 and 46 within treatments showed that individuals in the last stage demonstrated the highest intensities of lipid oxidative damage in both the control and desiccation groups. As for the parameters of the antioxidant system, control individuals displayed greater variety in response to changes induced by metamorphic climax than individuals exposed to desiccation treatment. The overall decrease in water availability during development led to increased oxidative stress and modifications in the pattern of AOS response to changes induced by metamorphic climax in larvae of B. variegata.",
publisher = "MDPI AG",
journal = "Animals",
title = "Effects of Desiccation on Metamorphic Climax in Bombina variegata: Changes in Levels and Patterns of Oxidative Stress Parameters",
number = "4",
volume = "11",
doi = "10.3390/ani11040953",
pages = "953"
}

Petrović, T., Kijanović, A., Tomašević Kolarov, N., Gavrić, J., Despotović, S., Gavrilović, B., Radovanović, T., Vukov, T., Faggio, C.,& Prokić, M.. (2021). Effects of Desiccation on Metamorphic Climax in Bombina variegata: Changes in Levels and Patterns of Oxidative Stress Parameters. in Animals
MDPI AG., 11(4), 953.
https://doi.org/10.3390/ani11040953

Petrović T, Kijanović A, Tomašević Kolarov N, Gavrić J, Despotović S, Gavrilović B, Radovanović T, Vukov T, Faggio C, Prokić M. Effects of Desiccation on Metamorphic Climax in Bombina variegata: Changes in Levels and Patterns of Oxidative Stress Parameters. in Animals. 2021;11(4):953.
doi:10.3390/ani11040953 .

Petrović, Tamara, Kijanović, Ana, Tomašević Kolarov, Nataša, Gavrić, Jelena, Despotović, Svetlana, Gavrilović, Branka, Radovanović, Tijana, Vukov, Tanja, Faggio, Caterina, Prokić, Marko, "Effects of Desiccation on Metamorphic Climax in Bombina variegata: Changes in Levels and Patterns of Oxidative Stress Parameters" in Animals, 11, no. 4 (2021):953,
https://doi.org/10.3390/ani11040953 . .

TY  - JOUR
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Petrović, Tamara
AU  - Ajduković, Maja
AU  - Vučić, Tijana
AU  - Cvijanović, Milena
AU  - Faggio, Caterina
AU  - Prokić, Marko
PY  - 2021
UR  - https://www.mdpi.com/1660-4601/18/18/9653
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8464833
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4483
AB  - Thiourea is an established disruptor of thyroid hormone synthesis and is frequently used as an inhibitor of metamorphosis. The changes caused by thiourea can affect processes associated with the oxidative status of individuals (metabolic rate, the HPI axis, antioxidant system). We investigated the parameters of oxidative stress in crested newt (Triturus spp.) larvae during normal development in late larval stage 62 and newly metamorphosed individuals, and during thiourea-stimulated metamorphosis arrest in individuals exposed to low (0.05%) and high (0.1%) concentrations of thiourea. Both groups of crested newts exposed to thiourea retained their larval characteristics until the end of the experiment. The low activities of antioxidant enzymes and the high lipid peroxidation level pointed to increased oxidative stress in larvae at the beginning of stage 62 as compared to fully metamorphosed individuals. The activities of catalase (CAT) and glutathione-S-transferase (GST) and the concentration of sulfhydryl (SH) groups were significantly lower in larvae reared in aqueous solutions containing thiourea than in newly metamorphosed individuals. The high thiourea concentration (0.1%) affected the antioxidative parameters to the extent that oxidative damage could not be avoided, contrary to a lower concentration. Our results provide a first insight into the physiological adaptations of crested newts during normal development and simulated metamorphosis arrest.
PB  - Basel: MDPI
T2  - International Journal of Environmental Research and Public Health
T1  - Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis.
IS  - 18
VL  - 18
DO  - 10.3390/ijerph18189653
SP  - 9653
ER  - 

@article{
author = "Gavrić, Jelena and Despotović, Svetlana and Gavrilović, Branka and Radovanović, Tijana and Petrović, Tamara and Ajduković, Maja and Vučić, Tijana and Cvijanović, Milena and Faggio, Caterina and Prokić, Marko",
year = "2021",
abstract = "Thiourea is an established disruptor of thyroid hormone synthesis and is frequently used as an inhibitor of metamorphosis. The changes caused by thiourea can affect processes associated with the oxidative status of individuals (metabolic rate, the HPI axis, antioxidant system). We investigated the parameters of oxidative stress in crested newt (Triturus spp.) larvae during normal development in late larval stage 62 and newly metamorphosed individuals, and during thiourea-stimulated metamorphosis arrest in individuals exposed to low (0.05%) and high (0.1%) concentrations of thiourea. Both groups of crested newts exposed to thiourea retained their larval characteristics until the end of the experiment. The low activities of antioxidant enzymes and the high lipid peroxidation level pointed to increased oxidative stress in larvae at the beginning of stage 62 as compared to fully metamorphosed individuals. The activities of catalase (CAT) and glutathione-S-transferase (GST) and the concentration of sulfhydryl (SH) groups were significantly lower in larvae reared in aqueous solutions containing thiourea than in newly metamorphosed individuals. The high thiourea concentration (0.1%) affected the antioxidative parameters to the extent that oxidative damage could not be avoided, contrary to a lower concentration. Our results provide a first insight into the physiological adaptations of crested newts during normal development and simulated metamorphosis arrest.",
publisher = "Basel: MDPI",
journal = "International Journal of Environmental Research and Public Health",
title = "Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis.",
number = "18",
volume = "18",
doi = "10.3390/ijerph18189653",
pages = "9653"
}

Gavrić, J., Despotović, S., Gavrilović, B., Radovanović, T., Petrović, T., Ajduković, M., Vučić, T., Cvijanović, M., Faggio, C.,& Prokić, M.. (2021). Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis.. in International Journal of Environmental Research and Public Health
Basel: MDPI., 18(18), 9653.
https://doi.org/10.3390/ijerph18189653

Gavrić J, Despotović S, Gavrilović B, Radovanović T, Petrović T, Ajduković M, Vučić T, Cvijanović M, Faggio C, Prokić M. Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis.. in International Journal of Environmental Research and Public Health. 2021;18(18):9653.
doi:10.3390/ijerph18189653 .

Gavrić, Jelena, Despotović, Svetlana, Gavrilović, Branka, Radovanović, Tijana, Petrović, Tamara, Ajduković, Maja, Vučić, Tijana, Cvijanović, Milena, Faggio, Caterina, Prokić, Marko, "Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis." in International Journal of Environmental Research and Public Health, 18, no. 18 (2021):9653,
https://doi.org/10.3390/ijerph18189653 . .

TY  - JOUR
AU  - Radovanović, Tijana
AU  - Gavrilović, Branka
AU  - Petrović, Tamara
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Kijanović, Ana
AU  - Mirč, Marko
AU  - Tomašević Kolarov, Nataša
AU  - Faggio, Caterina
AU  - Prokić, Marko
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S1532045621002180
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4484
AB  - Global warming represents a severe threat to existing ecosystems, especially for anuran tadpoles who encounter significant fluctuations in their habitats. Decreasing water levels in permanent and temporary water bodies is a significant risk for larval survival or fitness. On the other hand, the natural environment of amphibians is extremely polluted by various xenobiotics. This study evaluated how pre-exposure of Bombina variegata tadpoles to chronic environmental stress (desiccation) modulates the biochemical response of juvenile individuals to following acute chemical stressor (pesticide deltamethrin). Our results demonstrated that individually applied pesticide changed the thiol and lipid status of the treated juveniles but animals subjected solely to desiccation pressure were more tolerant to free radicals and showed no induction of lipid peroxidation. Comparison of juveniles exposed to deltamethrin revealed that desiccation pretreatment during the larval stage of development modified cellular protection in the juveniles. Higher activities of CAT, GSH-Px and GR were recorded in the pre-exposed group, as well as a lower degree of lipid peroxidation relative to the group that was not pre-exposed to low water stress. Pre-desiccated groups displayed the greatest range of coordination of investigated antioxidant parameters, supported by Pearson's correlations. Activation of the GSH-redox system is a significant marker in juveniles against stress caused by desiccation and a chemical stressor. The stressful environment experienced during tadpole development produced an adaptive reaction to subsequent exposure to another stressor in juveniles. To develop relevant management and conservation strategies, more studies of the interactive effects of environmental and chemical stressors are necessary.
PB  - New York: Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.
VL  - 250
DO  - 10.1016/j.cbpc.2021.109191
SP  - 109191
ER  - 

@article{
author = "Radovanović, Tijana and Gavrilović, Branka and Petrović, Tamara and Despotović, Svetlana and Gavrić, Jelena and Kijanović, Ana and Mirč, Marko and Tomašević Kolarov, Nataša and Faggio, Caterina and Prokić, Marko",
year = "2021",
abstract = "Global warming represents a severe threat to existing ecosystems, especially for anuran tadpoles who encounter significant fluctuations in their habitats. Decreasing water levels in permanent and temporary water bodies is a significant risk for larval survival or fitness. On the other hand, the natural environment of amphibians is extremely polluted by various xenobiotics. This study evaluated how pre-exposure of Bombina variegata tadpoles to chronic environmental stress (desiccation) modulates the biochemical response of juvenile individuals to following acute chemical stressor (pesticide deltamethrin). Our results demonstrated that individually applied pesticide changed the thiol and lipid status of the treated juveniles but animals subjected solely to desiccation pressure were more tolerant to free radicals and showed no induction of lipid peroxidation. Comparison of juveniles exposed to deltamethrin revealed that desiccation pretreatment during the larval stage of development modified cellular protection in the juveniles. Higher activities of CAT, GSH-Px and GR were recorded in the pre-exposed group, as well as a lower degree of lipid peroxidation relative to the group that was not pre-exposed to low water stress. Pre-desiccated groups displayed the greatest range of coordination of investigated antioxidant parameters, supported by Pearson's correlations. Activation of the GSH-redox system is a significant marker in juveniles against stress caused by desiccation and a chemical stressor. The stressful environment experienced during tadpole development produced an adaptive reaction to subsequent exposure to another stressor in juveniles. To develop relevant management and conservation strategies, more studies of the interactive effects of environmental and chemical stressors are necessary.",
publisher = "New York: Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.",
volume = "250",
doi = "10.1016/j.cbpc.2021.109191",
pages = "109191"
}

Radovanović, T., Gavrilović, B., Petrović, T., Despotović, S., Gavrić, J., Kijanović, A., Mirč, M., Tomašević Kolarov, N., Faggio, C.,& Prokić, M.. (2021). Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
New York: Elsevier Inc.., 250, 109191.
https://doi.org/10.1016/j.cbpc.2021.109191

Radovanović T, Gavrilović B, Petrović T, Despotović S, Gavrić J, Kijanović A, Mirč M, Tomašević Kolarov N, Faggio C, Prokić M. Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2021;250:109191.
doi:10.1016/j.cbpc.2021.109191 .

Radovanović, Tijana, Gavrilović, Branka, Petrović, Tamara, Despotović, Svetlana, Gavrić, Jelena, Kijanović, Ana, Mirč, Marko, Tomašević Kolarov, Nataša, Faggio, Caterina, Prokić, Marko, "Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles." in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 250 (2021):109191,
https://doi.org/10.1016/j.cbpc.2021.109191 . .

TY  - JOUR
AU  - Radovanović, Tijana
AU  - Gavrilović, Branka
AU  - Petrović, Tamara
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Kijanović, Ana
AU  - Mirč, Marko
AU  - Tomašević Kolarov, Nataša
AU  - Faggio, Caterina
AU  - Prokić, Marko
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S1532045621002180
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4484
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4497
AB  - Global warming represents a severe threat to existing ecosystems, especially for anuran tadpoles who encounter significant fluctuations in their habitats. Decreasing water levels in permanent and temporary water bodies is a significant risk for larval survival or fitness. On the other hand, the natural environment of amphibians is extremely polluted by various xenobiotics. This study evaluated how pre-exposure of Bombina variegata tadpoles to chronic environmental stress (desiccation) modulates the biochemical response of juvenile individuals to following acute chemical stressor (pesticide deltamethrin). Our results demonstrated that individually applied pesticide changed the thiol and lipid status of the treated juveniles but animals subjected solely to desiccation pressure were more tolerant to free radicals and showed no induction of lipid peroxidation. Comparison of juveniles exposed to deltamethrin revealed that desiccation pretreatment during the larval stage of development modified cellular protection in the juveniles. Higher activities of CAT, GSH-Px and GR were recorded in the pre-exposed group, as well as a lower degree of lipid peroxidation relative to the group that was not pre-exposed to low water stress. Pre-desiccated groups displayed the greatest range of coordination of investigated antioxidant parameters, supported by Pearson's correlations. Activation of the GSH-redox system is a significant marker in juveniles against stress caused by desiccation and a chemical stressor. The stressful environment experienced during tadpole development produced an adaptive reaction to subsequent exposure to another stressor in juveniles. To develop relevant management and conservation strategies, more studies of the interactive effects of environmental and chemical stressors are necessary.
PB  - New York: Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.
VL  - 250
DO  - 10.1016/j.cbpc.2021.109191
SP  - 109191
ER  - 

@article{
author = "Radovanović, Tijana and Gavrilović, Branka and Petrović, Tamara and Despotović, Svetlana and Gavrić, Jelena and Kijanović, Ana and Mirč, Marko and Tomašević Kolarov, Nataša and Faggio, Caterina and Prokić, Marko",
year = "2021",
abstract = "Global warming represents a severe threat to existing ecosystems, especially for anuran tadpoles who encounter significant fluctuations in their habitats. Decreasing water levels in permanent and temporary water bodies is a significant risk for larval survival or fitness. On the other hand, the natural environment of amphibians is extremely polluted by various xenobiotics. This study evaluated how pre-exposure of Bombina variegata tadpoles to chronic environmental stress (desiccation) modulates the biochemical response of juvenile individuals to following acute chemical stressor (pesticide deltamethrin). Our results demonstrated that individually applied pesticide changed the thiol and lipid status of the treated juveniles but animals subjected solely to desiccation pressure were more tolerant to free radicals and showed no induction of lipid peroxidation. Comparison of juveniles exposed to deltamethrin revealed that desiccation pretreatment during the larval stage of development modified cellular protection in the juveniles. Higher activities of CAT, GSH-Px and GR were recorded in the pre-exposed group, as well as a lower degree of lipid peroxidation relative to the group that was not pre-exposed to low water stress. Pre-desiccated groups displayed the greatest range of coordination of investigated antioxidant parameters, supported by Pearson's correlations. Activation of the GSH-redox system is a significant marker in juveniles against stress caused by desiccation and a chemical stressor. The stressful environment experienced during tadpole development produced an adaptive reaction to subsequent exposure to another stressor in juveniles. To develop relevant management and conservation strategies, more studies of the interactive effects of environmental and chemical stressors are necessary.",
publisher = "New York: Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.",
volume = "250",
doi = "10.1016/j.cbpc.2021.109191",
pages = "109191"
}

Radovanović, T., Gavrilović, B., Petrović, T., Despotović, S., Gavrić, J., Kijanović, A., Mirč, M., Tomašević Kolarov, N., Faggio, C.,& Prokić, M.. (2021). Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
New York: Elsevier Inc.., 250, 109191.
https://doi.org/10.1016/j.cbpc.2021.109191

Radovanović T, Gavrilović B, Petrović T, Despotović S, Gavrić J, Kijanović A, Mirč M, Tomašević Kolarov N, Faggio C, Prokić M. Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2021;250:109191.
doi:10.1016/j.cbpc.2021.109191 .

Radovanović, Tijana, Gavrilović, Branka, Petrović, Tamara, Despotović, Svetlana, Gavrić, Jelena, Kijanović, Ana, Mirč, Marko, Tomašević Kolarov, Nataša, Faggio, Caterina, Prokić, Marko, "Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles." in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 250 (2021):109191,
https://doi.org/10.1016/j.cbpc.2021.109191 . .

TY  - JOUR
AU  - Prokić, Marko
AU  - Petrović, Tamara
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Kijanović, Ana
AU  - Tomašević Kolarov, Nataša
AU  - Vukov, Tanja
AU  - Radovanović, Tijana
PY  - 2021
UR  - https://www.frontiersin.org/articles/10.3389/fphys.2021.783288/full
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8674722
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4757
AB  - Amphibians are sensitive to deteriorating environmental conditions, especially during transition to a terrestrial environment which is full of uncertainties. Harsh conditions, such as desiccation during earlier stages, affect different larval traits with possible carry-over effects on juvenile and adult life histories. The first consequences of the effects can be seen in juveniles in the challenges to find food and the ability to survive without it in a terrestrial habitat. Body size and the internal energy reserves acquired during the larval phase play an important role in this period. Herein, we tested how different water regimes (low water availability, desiccation and constant high-water availability) during larval development reflect on the oxidative status and ability of yellow belly toad (Bombina variegata) juveniles to endure short-term fasting. The desiccation regime significantly reduced the body size of metamorphs. The same was observed after 2 weeks of fasting, while the feeding treatment reduced differences mostly in the body mass of individuals from different water regimes. This was the result of a greater gain in mass in juveniles pre-exposed to desiccation. Pre-exposure to desiccation also modified the parameters of the antioxidant system (AOS) under feeding conditions, leading to higher values of superoxide dismutase, glutathione reductase and glutathione S-transferase, glutathione and sulfhydryl group concentrations, and lower glutathione peroxidase in comparison to juveniles reared under constant water. The increase in the AOS of juveniles can be considered as a physiological carry-over effect of desiccation, probably as the result of compensatory growth and/or earlier exposure to chronic stress. However, water levels during larval development did not exert significant effects on the oxidative status of juveniles subjected to food unavailability. Fasting juveniles, both control and desiccated, were exposed to oxidative stress, significantly higher lipid peroxide concentrations, lower superoxide dismutase, glutathione peroxidase, glutathione S-transferase, glutathione and sulfhydryl group values in comparison to feeding individuals. The lack of food in juvenile anurans activated the AOS response in the same manner, regardless of body size and stress pre-exposure, suggesting that the generally accepted hypothesis about the influence of metamorphic body size on the fitness of the postmetamorphic stage should be tested further.
T2  - Frontiers in Physiology
T1  - Carry-Over Effects of Desiccation Stress on the Oxidative Status of Fasting Anuran Juveniles.
VL  - 12
DO  - 10.3389/fphys.2021.783288
SP  - 783288
ER  - 

@article{
author = "Prokić, Marko and Petrović, Tamara and Gavrilović, Branka and Despotović, Svetlana and Gavrić, Jelena and Kijanović, Ana and Tomašević Kolarov, Nataša and Vukov, Tanja and Radovanović, Tijana",
year = "2021",
abstract = "Amphibians are sensitive to deteriorating environmental conditions, especially during transition to a terrestrial environment which is full of uncertainties. Harsh conditions, such as desiccation during earlier stages, affect different larval traits with possible carry-over effects on juvenile and adult life histories. The first consequences of the effects can be seen in juveniles in the challenges to find food and the ability to survive without it in a terrestrial habitat. Body size and the internal energy reserves acquired during the larval phase play an important role in this period. Herein, we tested how different water regimes (low water availability, desiccation and constant high-water availability) during larval development reflect on the oxidative status and ability of yellow belly toad (Bombina variegata) juveniles to endure short-term fasting. The desiccation regime significantly reduced the body size of metamorphs. The same was observed after 2 weeks of fasting, while the feeding treatment reduced differences mostly in the body mass of individuals from different water regimes. This was the result of a greater gain in mass in juveniles pre-exposed to desiccation. Pre-exposure to desiccation also modified the parameters of the antioxidant system (AOS) under feeding conditions, leading to higher values of superoxide dismutase, glutathione reductase and glutathione S-transferase, glutathione and sulfhydryl group concentrations, and lower glutathione peroxidase in comparison to juveniles reared under constant water. The increase in the AOS of juveniles can be considered as a physiological carry-over effect of desiccation, probably as the result of compensatory growth and/or earlier exposure to chronic stress. However, water levels during larval development did not exert significant effects on the oxidative status of juveniles subjected to food unavailability. Fasting juveniles, both control and desiccated, were exposed to oxidative stress, significantly higher lipid peroxide concentrations, lower superoxide dismutase, glutathione peroxidase, glutathione S-transferase, glutathione and sulfhydryl group values in comparison to feeding individuals. The lack of food in juvenile anurans activated the AOS response in the same manner, regardless of body size and stress pre-exposure, suggesting that the generally accepted hypothesis about the influence of metamorphic body size on the fitness of the postmetamorphic stage should be tested further.",
journal = "Frontiers in Physiology",
title = "Carry-Over Effects of Desiccation Stress on the Oxidative Status of Fasting Anuran Juveniles.",
volume = "12",
doi = "10.3389/fphys.2021.783288",
pages = "783288"
}

Prokić, M., Petrović, T., Gavrilović, B., Despotović, S., Gavrić, J., Kijanović, A., Tomašević Kolarov, N., Vukov, T.,& Radovanović, T.. (2021). Carry-Over Effects of Desiccation Stress on the Oxidative Status of Fasting Anuran Juveniles.. in Frontiers in Physiology, 12, 783288.
https://doi.org/10.3389/fphys.2021.783288

Prokić M, Petrović T, Gavrilović B, Despotović S, Gavrić J, Kijanović A, Tomašević Kolarov N, Vukov T, Radovanović T. Carry-Over Effects of Desiccation Stress on the Oxidative Status of Fasting Anuran Juveniles.. in Frontiers in Physiology. 2021;12:783288.
doi:10.3389/fphys.2021.783288 .

Prokić, Marko, Petrović, Tamara, Gavrilović, Branka, Despotović, Svetlana, Gavrić, Jelena, Kijanović, Ana, Tomašević Kolarov, Nataša, Vukov, Tanja, Radovanović, Tijana, "Carry-Over Effects of Desiccation Stress on the Oxidative Status of Fasting Anuran Juveniles." in Frontiers in Physiology, 12 (2021):783288,
https://doi.org/10.3389/fphys.2021.783288 . .

TY  - JOUR
AU  - Prokić, Marko
AU  - Petrović, Tamara
AU  - Despotović, Svetlana
AU  - Vučić, Tijana
AU  - Gavrić, Jelena
AU  - Radovanović, Tijana
AU  - Gavrilović, Branka
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S1095643320301720
UR  - https://radar.ibiss.bg.ac.rs/123456789/3902
UR  - https://radar.ibiss.bg.ac.rs/123456789/3903
AB  - In nature, animals often face periods without food caused by seasonal fluctuations and/or prey scarcity. An organism's physiological response to imposed energetic limitations is followed by changes in mitochondrial functioning (adjustment of energy metabolism) and a reduction of non-essential processes. However, this energy-saving strategy can have its costs. In this study, we examined oxidative stress as one of the possible physiological costs of short-term, two-week-long food deprivation on developing amphibian larvae of the crested newts Triturus macedonicus and Triturus ivanbureschi and their hybrids. We investigated whether this exogenous factor additionally affected the oxidative status (fitness-related trait) of hybrid individuals. The fasting treatment led to lower growth and a lower body mass and body condition index of individuals. The results revealed that the antioxidant system (AOS) of food-deprived larvae could not cope in a proper manner with reactive oxygen species production under limited energy availability, leading to higher lipid oxidative damage. The lowest AOS response was observed for H2O2 scavenging parameters (catalase, glutathione peroxidase, and total glutathione), which together with the elevated activity of superoxide dismutase suggested increased H2O2 concentrations. Comparison between parental species and their hybrids showed that hybrid individuals suffered greater oxidative damage (as demonstrated by higher concentrations of lipid peroxides), indicating that they were more susceptible to fasting-induced oxidative stress. Overall, this study illustrates that: (i) an oxidative event is one of the costs amphibian larvae face during short-term periods of fasting, (ii) hybrids are less capable of dealing with this stressful condition, which can lower their chances of survival in a changing environment.
PB  - Pergamon
T2  - Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
T1  - The effect of short-term fasting on the oxidative status of larvae of crested newt species and their hybrids
VL  - 251
DO  - 10.1016/j.cbpa.2020.110819
SP  - 110819
ER  - 

@article{
author = "Prokić, Marko and Petrović, Tamara and Despotović, Svetlana and Vučić, Tijana and Gavrić, Jelena and Radovanović, Tijana and Gavrilović, Branka",
year = "2021",
abstract = "In nature, animals often face periods without food caused by seasonal fluctuations and/or prey scarcity. An organism's physiological response to imposed energetic limitations is followed by changes in mitochondrial functioning (adjustment of energy metabolism) and a reduction of non-essential processes. However, this energy-saving strategy can have its costs. In this study, we examined oxidative stress as one of the possible physiological costs of short-term, two-week-long food deprivation on developing amphibian larvae of the crested newts Triturus macedonicus and Triturus ivanbureschi and their hybrids. We investigated whether this exogenous factor additionally affected the oxidative status (fitness-related trait) of hybrid individuals. The fasting treatment led to lower growth and a lower body mass and body condition index of individuals. The results revealed that the antioxidant system (AOS) of food-deprived larvae could not cope in a proper manner with reactive oxygen species production under limited energy availability, leading to higher lipid oxidative damage. The lowest AOS response was observed for H2O2 scavenging parameters (catalase, glutathione peroxidase, and total glutathione), which together with the elevated activity of superoxide dismutase suggested increased H2O2 concentrations. Comparison between parental species and their hybrids showed that hybrid individuals suffered greater oxidative damage (as demonstrated by higher concentrations of lipid peroxides), indicating that they were more susceptible to fasting-induced oxidative stress. Overall, this study illustrates that: (i) an oxidative event is one of the costs amphibian larvae face during short-term periods of fasting, (ii) hybrids are less capable of dealing with this stressful condition, which can lower their chances of survival in a changing environment.",
publisher = "Pergamon",
journal = "Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology",
title = "The effect of short-term fasting on the oxidative status of larvae of crested newt species and their hybrids",
volume = "251",
doi = "10.1016/j.cbpa.2020.110819",
pages = "110819"
}

Prokić, M., Petrović, T., Despotović, S., Vučić, T., Gavrić, J., Radovanović, T.,& Gavrilović, B.. (2021). The effect of short-term fasting on the oxidative status of larvae of crested newt species and their hybrids. in Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
Pergamon., 251, 110819.
https://doi.org/10.1016/j.cbpa.2020.110819

Prokić M, Petrović T, Despotović S, Vučić T, Gavrić J, Radovanović T, Gavrilović B. The effect of short-term fasting on the oxidative status of larvae of crested newt species and their hybrids. in Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. 2021;251:110819.
doi:10.1016/j.cbpa.2020.110819 .

Prokić, Marko, Petrović, Tamara, Despotović, Svetlana, Vučić, Tijana, Gavrić, Jelena, Radovanović, Tijana, Gavrilović, Branka, "The effect of short-term fasting on the oxidative status of larvae of crested newt species and their hybrids" in Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 251 (2021):110819,
https://doi.org/10.1016/j.cbpa.2020.110819 . .

TY  - JOUR
AU  - Prokić, Marko
AU  - Petrović, Tamara
AU  - Despotović, Svetlana
AU  - Vučić, Tijana
AU  - Gavrić, Jelena
AU  - Radovanović, Tijana
AU  - Gavrilović, Branka
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S1095643320301720
UR  - https://radar.ibiss.bg.ac.rs/123456789/3902
AB  - In nature, animals often face periods without food caused by seasonal fluctuations and/or prey scarcity. An organism's physiological response to imposed energetic limitations is followed by changes in mitochondrial functioning (adjustment of energy metabolism) and a reduction of non-essential processes. However, this energy-saving strategy can have its costs. In this study, we examined oxidative stress as one of the possible physiological costs of short-term, two-week-long food deprivation on developing amphibian larvae of the crested newts Triturus macedonicus and Triturus ivanbureschi and their hybrids. We investigated whether this exogenous factor additionally affected the oxidative status (fitness-related trait) of hybrid individuals. The fasting treatment led to lower growth and a lower body mass and body condition index of individuals. The results revealed that the antioxidant system (AOS) of food-deprived larvae could not cope in a proper manner with reactive oxygen species production under limited energy availability, leading to higher lipid oxidative damage. The lowest AOS response was observed for H2O2 scavenging parameters (catalase, glutathione peroxidase, and total glutathione), which together with the elevated activity of superoxide dismutase suggested increased H2O2 concentrations. Comparison between parental species and their hybrids showed that hybrid individuals suffered greater oxidative damage (as demonstrated by higher concentrations of lipid peroxides), indicating that they were more susceptible to fasting-induced oxidative stress. Overall, this study illustrates that: (i) an oxidative event is one of the costs amphibian larvae face during short-term periods of fasting, (ii) hybrids are less capable of dealing with this stressful condition, which can lower their chances of survival in a changing environment.
PB  - Pergamon
T2  - Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
T1  - The effect of short-term fasting on the oxidative status of larvae of crested newt species and their hybrids
VL  - 251
DO  - 10.1016/j.cbpa.2020.110819
SP  - 110819
ER  - 

@article{
author = "Prokić, Marko and Petrović, Tamara and Despotović, Svetlana and Vučić, Tijana and Gavrić, Jelena and Radovanović, Tijana and Gavrilović, Branka",
year = "2021",
abstract = "In nature, animals often face periods without food caused by seasonal fluctuations and/or prey scarcity. An organism's physiological response to imposed energetic limitations is followed by changes in mitochondrial functioning (adjustment of energy metabolism) and a reduction of non-essential processes. However, this energy-saving strategy can have its costs. In this study, we examined oxidative stress as one of the possible physiological costs of short-term, two-week-long food deprivation on developing amphibian larvae of the crested newts Triturus macedonicus and Triturus ivanbureschi and their hybrids. We investigated whether this exogenous factor additionally affected the oxidative status (fitness-related trait) of hybrid individuals. The fasting treatment led to lower growth and a lower body mass and body condition index of individuals. The results revealed that the antioxidant system (AOS) of food-deprived larvae could not cope in a proper manner with reactive oxygen species production under limited energy availability, leading to higher lipid oxidative damage. The lowest AOS response was observed for H2O2 scavenging parameters (catalase, glutathione peroxidase, and total glutathione), which together with the elevated activity of superoxide dismutase suggested increased H2O2 concentrations. Comparison between parental species and their hybrids showed that hybrid individuals suffered greater oxidative damage (as demonstrated by higher concentrations of lipid peroxides), indicating that they were more susceptible to fasting-induced oxidative stress. Overall, this study illustrates that: (i) an oxidative event is one of the costs amphibian larvae face during short-term periods of fasting, (ii) hybrids are less capable of dealing with this stressful condition, which can lower their chances of survival in a changing environment.",
publisher = "Pergamon",
journal = "Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology",
title = "The effect of short-term fasting on the oxidative status of larvae of crested newt species and their hybrids",
volume = "251",
doi = "10.1016/j.cbpa.2020.110819",
pages = "110819"
}

Prokić, M., Petrović, T., Despotović, S., Vučić, T., Gavrić, J., Radovanović, T.,& Gavrilović, B.. (2021). The effect of short-term fasting on the oxidative status of larvae of crested newt species and their hybrids. in Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
Pergamon., 251, 110819.
https://doi.org/10.1016/j.cbpa.2020.110819

Prokić M, Petrović T, Despotović S, Vučić T, Gavrić J, Radovanović T, Gavrilović B. The effect of short-term fasting on the oxidative status of larvae of crested newt species and their hybrids. in Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. 2021;251:110819.
doi:10.1016/j.cbpa.2020.110819 .

Prokić, Marko, Petrović, Tamara, Despotović, Svetlana, Vučić, Tijana, Gavrić, Jelena, Radovanović, Tijana, Gavrilović, Branka, "The effect of short-term fasting on the oxidative status of larvae of crested newt species and their hybrids" in Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 251 (2021):110819,
https://doi.org/10.1016/j.cbpa.2020.110819 . .

TY  - JOUR
AU  - Gavrilović, Branka
AU  - Petrović, Tamara
AU  - Radovanović, Tijana
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Krizmanić, Imre I.
AU  - Ćirić, Miloš D.
AU  - Prokić, Marko
PY  - 2021
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32853936
UR  - https://radar.ibiss.bg.ac.rs/123456789/3856
UR  - https://radar.ibiss.bg.ac.rs/123456789/3857
AB  - Although the long-term exposure of aquatic organisms to cyanobacterial blooms is a regular occurrence in the environment, the prooxidant and neurotoxic effects of such conditions are still insufficiently investigated in situ. We examined the temporal dynamics of the biochemical parameters in the liver of Pelophylax kl. esculentus frogs that inhabit the northern (N) side of Lake Ludaš (Serbia) with microcystins (MCs) produced in a cyanobacterial bloom over three summer months. The obtained data were compared with data on frogs that live on the southern (S), MC-free side of the same lake. Our results showed that the MC-producing bloom induced oxidative damage to proteins and lipids, observed as a decrease in the concentration of protein -SH groups and increased lipid peroxidation (LPO) in the liver of N frogs in comparison to S frogs. Glutathione (GSH) played a key role in the transient defense against the MC-induced development of LPO. The low glutathione peroxidase (GPx) activity detected in all groups of frogs from the N site was crucial for the observed prooxidant consequences. The bloom impaired cholinergic homeostasis as a result of a decrease in ChE activity. A delayed neurotoxic effect in relation to the prooxidant outcomes was observed. Our results also showed that even though the integrated biomarker response (IBR) of the antioxidant biomarkers increased during exposure, the individual biochemical parameters did not exhibit a well-defined time-dependent pattern because of specific adaptation dynamics and/or additional effects of the physicochemical parameters of the water. This comprehensive environmental ecotoxicological evaluation of the cyanobacterial bloom-induced biochemical alterations in the liver of frogs provides a new basis for further investigations of the prolonged, real-life ecotoxicity of the blooms.
PB  - Elsevier B.V.
T2  - Science of the Total Environment
T1  - Hepatic oxidative stress and neurotoxicity in Pelophylax kl. esculentus frogs: Influence of long-term exposure to a cyanobacterial bloom.
VL  - 750
DO  - 10.1016/j.scitotenv.2020.141569
SP  - 141569
ER  - 

@article{
author = "Gavrilović, Branka and Petrović, Tamara and Radovanović, Tijana and Despotović, Svetlana and Gavrić, Jelena and Krizmanić, Imre I. and Ćirić, Miloš D. and Prokić, Marko",
year = "2021",
abstract = "Although the long-term exposure of aquatic organisms to cyanobacterial blooms is a regular occurrence in the environment, the prooxidant and neurotoxic effects of such conditions are still insufficiently investigated in situ. We examined the temporal dynamics of the biochemical parameters in the liver of Pelophylax kl. esculentus frogs that inhabit the northern (N) side of Lake Ludaš (Serbia) with microcystins (MCs) produced in a cyanobacterial bloom over three summer months. The obtained data were compared with data on frogs that live on the southern (S), MC-free side of the same lake. Our results showed that the MC-producing bloom induced oxidative damage to proteins and lipids, observed as a decrease in the concentration of protein -SH groups and increased lipid peroxidation (LPO) in the liver of N frogs in comparison to S frogs. Glutathione (GSH) played a key role in the transient defense against the MC-induced development of LPO. The low glutathione peroxidase (GPx) activity detected in all groups of frogs from the N site was crucial for the observed prooxidant consequences. The bloom impaired cholinergic homeostasis as a result of a decrease in ChE activity. A delayed neurotoxic effect in relation to the prooxidant outcomes was observed. Our results also showed that even though the integrated biomarker response (IBR) of the antioxidant biomarkers increased during exposure, the individual biochemical parameters did not exhibit a well-defined time-dependent pattern because of specific adaptation dynamics and/or additional effects of the physicochemical parameters of the water. This comprehensive environmental ecotoxicological evaluation of the cyanobacterial bloom-induced biochemical alterations in the liver of frogs provides a new basis for further investigations of the prolonged, real-life ecotoxicity of the blooms.",
publisher = "Elsevier B.V.",
journal = "Science of the Total Environment",
title = "Hepatic oxidative stress and neurotoxicity in Pelophylax kl. esculentus frogs: Influence of long-term exposure to a cyanobacterial bloom.",
volume = "750",
doi = "10.1016/j.scitotenv.2020.141569",
pages = "141569"
}

Gavrilović, B., Petrović, T., Radovanović, T., Despotović, S., Gavrić, J., Krizmanić, I. I., Ćirić, M. D.,& Prokić, M.. (2021). Hepatic oxidative stress and neurotoxicity in Pelophylax kl. esculentus frogs: Influence of long-term exposure to a cyanobacterial bloom.. in Science of the Total Environment
Elsevier B.V.., 750, 141569.
https://doi.org/10.1016/j.scitotenv.2020.141569

Gavrilović B, Petrović T, Radovanović T, Despotović S, Gavrić J, Krizmanić II, Ćirić MD, Prokić M. Hepatic oxidative stress and neurotoxicity in Pelophylax kl. esculentus frogs: Influence of long-term exposure to a cyanobacterial bloom.. in Science of the Total Environment. 2021;750:141569.
doi:10.1016/j.scitotenv.2020.141569 .

Gavrilović, Branka, Petrović, Tamara, Radovanović, Tijana, Despotović, Svetlana, Gavrić, Jelena, Krizmanić, Imre I., Ćirić, Miloš D., Prokić, Marko, "Hepatic oxidative stress and neurotoxicity in Pelophylax kl. esculentus frogs: Influence of long-term exposure to a cyanobacterial bloom." in Science of the Total Environment, 750 (2021):141569,
https://doi.org/10.1016/j.scitotenv.2020.141569 . .

TY  - JOUR
AU  - Gavrilović, Branka
AU  - Petrović, Tamara
AU  - Radovanović, Tijana
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Krizmanić, Imre I.
AU  - Ćirić, Miloš D.
AU  - Prokić, Marko
PY  - 2021
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32853936
UR  - https://radar.ibiss.bg.ac.rs/123456789/3856
AB  - Although the long-term exposure of aquatic organisms to cyanobacterial blooms is a regular occurrence in the environment, the prooxidant and neurotoxic effects of such conditions are still insufficiently investigated in situ. We examined the temporal dynamics of the biochemical parameters in the liver of Pelophylax kl. esculentus frogs that inhabit the northern (N) side of Lake Ludaš (Serbia) with microcystins (MCs) produced in a cyanobacterial bloom over three summer months. The obtained data were compared with data on frogs that live on the southern (S), MC-free side of the same lake. Our results showed that the MC-producing bloom induced oxidative damage to proteins and lipids, observed as a decrease in the concentration of protein -SH groups and increased lipid peroxidation (LPO) in the liver of N frogs in comparison to S frogs. Glutathione (GSH) played a key role in the transient defense against the MC-induced development of LPO. The low glutathione peroxidase (GPx) activity detected in all groups of frogs from the N site was crucial for the observed prooxidant consequences. The bloom impaired cholinergic homeostasis as a result of a decrease in ChE activity. A delayed neurotoxic effect in relation to the prooxidant outcomes was observed. Our results also showed that even though the integrated biomarker response (IBR) of the antioxidant biomarkers increased during exposure, the individual biochemical parameters did not exhibit a well-defined time-dependent pattern because of specific adaptation dynamics and/or additional effects of the physicochemical parameters of the water. This comprehensive environmental ecotoxicological evaluation of the cyanobacterial bloom-induced biochemical alterations in the liver of frogs provides a new basis for further investigations of the prolonged, real-life ecotoxicity of the blooms.
PB  - Elsevier B.V.
T2  - Science of the Total Environment
T1  - Hepatic oxidative stress and neurotoxicity in Pelophylax kl. esculentus frogs: Influence of long-term exposure to a cyanobacterial bloom.
VL  - 750
DO  - 10.1016/j.scitotenv.2020.141569
SP  - 141569
ER  - 

@article{
author = "Gavrilović, Branka and Petrović, Tamara and Radovanović, Tijana and Despotović, Svetlana and Gavrić, Jelena and Krizmanić, Imre I. and Ćirić, Miloš D. and Prokić, Marko",
year = "2021",
abstract = "Although the long-term exposure of aquatic organisms to cyanobacterial blooms is a regular occurrence in the environment, the prooxidant and neurotoxic effects of such conditions are still insufficiently investigated in situ. We examined the temporal dynamics of the biochemical parameters in the liver of Pelophylax kl. esculentus frogs that inhabit the northern (N) side of Lake Ludaš (Serbia) with microcystins (MCs) produced in a cyanobacterial bloom over three summer months. The obtained data were compared with data on frogs that live on the southern (S), MC-free side of the same lake. Our results showed that the MC-producing bloom induced oxidative damage to proteins and lipids, observed as a decrease in the concentration of protein -SH groups and increased lipid peroxidation (LPO) in the liver of N frogs in comparison to S frogs. Glutathione (GSH) played a key role in the transient defense against the MC-induced development of LPO. The low glutathione peroxidase (GPx) activity detected in all groups of frogs from the N site was crucial for the observed prooxidant consequences. The bloom impaired cholinergic homeostasis as a result of a decrease in ChE activity. A delayed neurotoxic effect in relation to the prooxidant outcomes was observed. Our results also showed that even though the integrated biomarker response (IBR) of the antioxidant biomarkers increased during exposure, the individual biochemical parameters did not exhibit a well-defined time-dependent pattern because of specific adaptation dynamics and/or additional effects of the physicochemical parameters of the water. This comprehensive environmental ecotoxicological evaluation of the cyanobacterial bloom-induced biochemical alterations in the liver of frogs provides a new basis for further investigations of the prolonged, real-life ecotoxicity of the blooms.",
publisher = "Elsevier B.V.",
journal = "Science of the Total Environment",
title = "Hepatic oxidative stress and neurotoxicity in Pelophylax kl. esculentus frogs: Influence of long-term exposure to a cyanobacterial bloom.",
volume = "750",
doi = "10.1016/j.scitotenv.2020.141569",
pages = "141569"
}

Gavrilović, B., Petrović, T., Radovanović, T., Despotović, S., Gavrić, J., Krizmanić, I. I., Ćirić, M. D.,& Prokić, M.. (2021). Hepatic oxidative stress and neurotoxicity in Pelophylax kl. esculentus frogs: Influence of long-term exposure to a cyanobacterial bloom.. in Science of the Total Environment
Elsevier B.V.., 750, 141569.
https://doi.org/10.1016/j.scitotenv.2020.141569

Gavrilović B, Petrović T, Radovanović T, Despotović S, Gavrić J, Krizmanić II, Ćirić MD, Prokić M. Hepatic oxidative stress and neurotoxicity in Pelophylax kl. esculentus frogs: Influence of long-term exposure to a cyanobacterial bloom.. in Science of the Total Environment. 2021;750:141569.
doi:10.1016/j.scitotenv.2020.141569 .

Gavrilović, Branka, Petrović, Tamara, Radovanović, Tijana, Despotović, Svetlana, Gavrić, Jelena, Krizmanić, Imre I., Ćirić, Miloš D., Prokić, Marko, "Hepatic oxidative stress and neurotoxicity in Pelophylax kl. esculentus frogs: Influence of long-term exposure to a cyanobacterial bloom." in Science of the Total Environment, 750 (2021):141569,
https://doi.org/10.1016/j.scitotenv.2020.141569 . .

TY  - THES
AU  - Petrović, Tamara
PY  - 2020
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=7624
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:22618/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=20973065
UR  - http://nardus.mpn.gov.rs/handle/123456789/17470
UR  - https://radar.ibiss.bg.ac.rs/123456789/3911
AB  - U ovoj studiji sumirani su inter- i intraspecijski aspekti morfološke varijabilnosti ekstremiteta bezrepih vodozemaca pod uticajem selekcionih pritisaka vezanih za ulogu ekstremiteta u lokomociji i reprodukciji. Varijabilnost elemenata ekstremiteta je u direktnoj vezi sa načinom kretanja i sa stepenom korišćenja akvatične, terestrične i arborealne sredine, ali i u indirektnoj vezi u pogledu razlika između polova i osobina životnih istorija. Analizom polnog dimorfizma, utvrđene su razlike u veličini i obliku prednjih ekstremiteta, dok neznatne razlike u zadnjim ekstremitetima i elementima sakralnog pršljena ukazuju na postojanje ograničenja u nastanku intraspecijske varijabilnosti u ovim osobinama kod bezrepih vodozemaca, verovatno zbog jakih biomehaničkih zahteva visoko specijalizovane lokomocije. Poređenjem morfološke varijabilnosti različitih lokomotornih tipova (skakača i hodača) potvrđen je važan uticaj zadnjih ekstremiteta, i istaknut uticaj proksimalnog i medijalnog dela prednjih ekstremiteta, kao i osobina karličnog pršljena u kretanju i definisanju morfologije lokomotornih tipova Anura. Analizom morfološke integracije pokazano je da najveći uticaj na korelacionu strukturu ekstremiteta bezrepih vodozemaca ima prirodna selekcija, pre svega kroz specijalizaciju za skok, a zatim i kroz druge lokomotorne aktivnosti koje su povezane sa različitim preferencama prema tipu staništa i načinu života vrsta. Sa druge strane, pored postojanja veze između filogenetske istorije vrsta i ekoloških preferenci, nije utvrđen značajan uticaj filogenetskih odnosa vrsta na nivo i obrasce korelacija ekstremiteta. Ova studija je pokazala značajan uticaj lokomocije i reprodukcije u oblikovanju varijabilnosti i razumevanju faktora evolucionih promena ekstremiteta bezrepih vodozemaca.
AB  - This study summarized inter- and intraspecific morphological variability of anuran limbs in relation to selective pressures of locomotion and reproduction. Morphological variability of limb traits is directly related to the locomotor mode used in aquatic, terrestrial and arboreal environments, and indirectly to the differences between the sexes and the features of life histories. Analyses of sexual dimorphism of locomotor traits revealed differences in size and shape only for forelimbs. The absence of significant differences for hindlimb and sacral traits indicate constraints in intraspecific variability of these traits, which can be result of specialization to saltatorial locomotion. Comparison of the morphological variability of locomotor types (walkers and jumpers) highlighted the importance of hindlimbs, the proximal and medial elements of the forelimbs, as well as the features of the pelvic vertebra, in movement and definition of the morphology of the locomotor types in Anurans. The analysis of morphological integration showed that natural selection has the highest influence on the correlation structure of anurans, archived mainly through the specialization for jump, but also through other locomotor activities that are related to different preferences for habitat type. Even though there was a relationship between phylogenetic history of species and ecological preferences significant influence of phylogenetic relationships of species on the level and patterns of limb correlations was not observed. In conclusion, this study demonstrated the significant influence of locomotion and reproduction in shaping variability of limbs of anurans, contributing to the understanding of evolutionary changes that this group went through.
PB  - Belgrade: University of Belgrade, Faculty of Biology
T2  - University of Belgrade, Faculty of Biology
T1  - Morfološka varijabilnost i obrasci korelacija elemenata ekstremiteta bezrepih vodozemaca (Anura, Amphibia)
T1  - Morphological variability and patterns of correlation in limb elements of tailess amphibians (Anura, Amphibia)
SP  - 1
EP  - 65
UR  - https://hdl.handle.net/21.15107/rcub_nardus_17470
ER  - 

@phdthesis{
author = "Petrović, Tamara",
year = "2020",
abstract = "U ovoj studiji sumirani su inter- i intraspecijski aspekti morfološke varijabilnosti ekstremiteta bezrepih vodozemaca pod uticajem selekcionih pritisaka vezanih za ulogu ekstremiteta u lokomociji i reprodukciji. Varijabilnost elemenata ekstremiteta je u direktnoj vezi sa načinom kretanja i sa stepenom korišćenja akvatične, terestrične i arborealne sredine, ali i u indirektnoj vezi u pogledu razlika između polova i osobina životnih istorija. Analizom polnog dimorfizma, utvrđene su razlike u veličini i obliku prednjih ekstremiteta, dok neznatne razlike u zadnjim ekstremitetima i elementima sakralnog pršljena ukazuju na postojanje ograničenja u nastanku intraspecijske varijabilnosti u ovim osobinama kod bezrepih vodozemaca, verovatno zbog jakih biomehaničkih zahteva visoko specijalizovane lokomocije. Poređenjem morfološke varijabilnosti različitih lokomotornih tipova (skakača i hodača) potvrđen je važan uticaj zadnjih ekstremiteta, i istaknut uticaj proksimalnog i medijalnog dela prednjih ekstremiteta, kao i osobina karličnog pršljena u kretanju i definisanju morfologije lokomotornih tipova Anura. Analizom morfološke integracije pokazano je da najveći uticaj na korelacionu strukturu ekstremiteta bezrepih vodozemaca ima prirodna selekcija, pre svega kroz specijalizaciju za skok, a zatim i kroz druge lokomotorne aktivnosti koje su povezane sa različitim preferencama prema tipu staništa i načinu života vrsta. Sa druge strane, pored postojanja veze između filogenetske istorije vrsta i ekoloških preferenci, nije utvrđen značajan uticaj filogenetskih odnosa vrsta na nivo i obrasce korelacija ekstremiteta. Ova studija je pokazala značajan uticaj lokomocije i reprodukcije u oblikovanju varijabilnosti i razumevanju faktora evolucionih promena ekstremiteta bezrepih vodozemaca., This study summarized inter- and intraspecific morphological variability of anuran limbs in relation to selective pressures of locomotion and reproduction. Morphological variability of limb traits is directly related to the locomotor mode used in aquatic, terrestrial and arboreal environments, and indirectly to the differences between the sexes and the features of life histories. Analyses of sexual dimorphism of locomotor traits revealed differences in size and shape only for forelimbs. The absence of significant differences for hindlimb and sacral traits indicate constraints in intraspecific variability of these traits, which can be result of specialization to saltatorial locomotion. Comparison of the morphological variability of locomotor types (walkers and jumpers) highlighted the importance of hindlimbs, the proximal and medial elements of the forelimbs, as well as the features of the pelvic vertebra, in movement and definition of the morphology of the locomotor types in Anurans. The analysis of morphological integration showed that natural selection has the highest influence on the correlation structure of anurans, archived mainly through the specialization for jump, but also through other locomotor activities that are related to different preferences for habitat type. Even though there was a relationship between phylogenetic history of species and ecological preferences significant influence of phylogenetic relationships of species on the level and patterns of limb correlations was not observed. In conclusion, this study demonstrated the significant influence of locomotion and reproduction in shaping variability of limbs of anurans, contributing to the understanding of evolutionary changes that this group went through.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "University of Belgrade, Faculty of Biology",
title = "Morfološka varijabilnost i obrasci korelacija elemenata ekstremiteta bezrepih vodozemaca (Anura, Amphibia), Morphological variability and patterns of correlation in limb elements of tailess amphibians (Anura, Amphibia)",
pages = "1-65",
url = "https://hdl.handle.net/21.15107/rcub_nardus_17470"
}

Petrović, T.. (2020). Morfološka varijabilnost i obrasci korelacija elemenata ekstremiteta bezrepih vodozemaca (Anura, Amphibia). in University of Belgrade, Faculty of Biology
Belgrade: University of Belgrade, Faculty of Biology., 1-65.
https://hdl.handle.net/21.15107/rcub_nardus_17470

Petrović T. Morfološka varijabilnost i obrasci korelacija elemenata ekstremiteta bezrepih vodozemaca (Anura, Amphibia). in University of Belgrade, Faculty of Biology. 2020;:1-65.
https://hdl.handle.net/21.15107/rcub_nardus_17470 .

Petrović, Tamara, "Morfološka varijabilnost i obrasci korelacija elemenata ekstremiteta bezrepih vodozemaca (Anura, Amphibia)" in University of Belgrade, Faculty of Biology (2020):1-65,
https://hdl.handle.net/21.15107/rcub_nardus_17470 .

TY  - JOUR
AU  - Petrović, Tamara
AU  - Vučić, Tijana
AU  - Nikolić, Sonja Z.
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Faggio, Caterina
AU  - Prokić, Marko
PY  - 2020
UR  - https://www.mdpi.com/2076-2615/10/4/603
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3649
AB  - Shelters are important for animal survival. Provision of adequate hiding places allow animals to express their natural sheltering behavior and it can have different positive effects on cortisol levels, physiological processes and mental performance. Although the absence of a refuge activates some stress response, its effect on oxidative stress has not been adequately examined. This study investigated whether the presence/absence of a shelter modifies the oxidative status (the antioxidant system and oxidative damage) and aggressive behavior of crested newt larvae (Triturus macedonicus and its hybrid with T. ivanbureschi). Our results show that individuals reared with shelters had lower values of the tested antioxidant parameters (catalase, glutathione peroxidase, glutathione S-transferase and glutathione), indicating a lower production of reactive species than individuals reared without shelter. The same pattern was observed in both T. macedonicus and its hybrid. Contrary to the activation of some physiological pathways, shelter availability did not significantly affect the rate of intraspecific aggressive behavior. The physiological benefits of shelter use can be manifested as a lower requirement for investment in the energy necessary for the maintenance of the upregulated antioxidant defenses, activation of repair systems and synthesis of endogenous antioxidants. This study highlights the importance of shelter provision, which may be valuable in habitat restoration and animal conservation studies.
T2  - Animals
T1  - The Effect of Shelter on Oxidative Stress and Aggressive Behavior in Crested Newt Larvae (Triturus spp.)
IS  - 4
VL  - 10
DO  - 10.3390/ani10040603
SP  - 603
ER  - 

@article{
author = "Petrović, Tamara and Vučić, Tijana and Nikolić, Sonja Z. and Gavrić, Jelena and Despotović, Svetlana and Gavrilović, Branka and Radovanović, Tijana and Faggio, Caterina and Prokić, Marko",
year = "2020",
abstract = "Shelters are important for animal survival. Provision of adequate hiding places allow animals to express their natural sheltering behavior and it can have different positive effects on cortisol levels, physiological processes and mental performance. Although the absence of a refuge activates some stress response, its effect on oxidative stress has not been adequately examined. This study investigated whether the presence/absence of a shelter modifies the oxidative status (the antioxidant system and oxidative damage) and aggressive behavior of crested newt larvae (Triturus macedonicus and its hybrid with T. ivanbureschi). Our results show that individuals reared with shelters had lower values of the tested antioxidant parameters (catalase, glutathione peroxidase, glutathione S-transferase and glutathione), indicating a lower production of reactive species than individuals reared without shelter. The same pattern was observed in both T. macedonicus and its hybrid. Contrary to the activation of some physiological pathways, shelter availability did not significantly affect the rate of intraspecific aggressive behavior. The physiological benefits of shelter use can be manifested as a lower requirement for investment in the energy necessary for the maintenance of the upregulated antioxidant defenses, activation of repair systems and synthesis of endogenous antioxidants. This study highlights the importance of shelter provision, which may be valuable in habitat restoration and animal conservation studies.",
journal = "Animals",
title = "The Effect of Shelter on Oxidative Stress and Aggressive Behavior in Crested Newt Larvae (Triturus spp.)",
number = "4",
volume = "10",
doi = "10.3390/ani10040603",
pages = "603"
}

Petrović, T., Vučić, T., Nikolić, S. Z., Gavrić, J., Despotović, S., Gavrilović, B., Radovanović, T., Faggio, C.,& Prokić, M.. (2020). The Effect of Shelter on Oxidative Stress and Aggressive Behavior in Crested Newt Larvae (Triturus spp.). in Animals, 10(4), 603.
https://doi.org/10.3390/ani10040603

Petrović T, Vučić T, Nikolić SZ, Gavrić J, Despotović S, Gavrilović B, Radovanović T, Faggio C, Prokić M. The Effect of Shelter on Oxidative Stress and Aggressive Behavior in Crested Newt Larvae (Triturus spp.). in Animals. 2020;10(4):603.
doi:10.3390/ani10040603 .

Petrović, Tamara, Vučić, Tijana, Nikolić, Sonja Z., Gavrić, Jelena, Despotović, Svetlana, Gavrilović, Branka, Radovanović, Tijana, Faggio, Caterina, Prokić, Marko, "The Effect of Shelter on Oxidative Stress and Aggressive Behavior in Crested Newt Larvae (Triturus spp.)" in Animals, 10, no. 4 (2020):603,
https://doi.org/10.3390/ani10040603 . .

TY  - JOUR
AU  - Gavrilović, Branka
AU  - Prokić, Marko
AU  - Petrović, Tamara
AU  - Despotović, Svetlana
AU  - Radovanović, Tijana
AU  - Krizmanić, Imre
AU  - Ćirić, Miloš
AU  - Gavrić, Jelena
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3794
AB  - There is little information in scientific literature as to how conditions created by a microcystin (MC) producing cyanobacterial bloom affect the oxidant/antioxidant, biotransformation and neurotoxicity parameters in adult frogs in situ. We investigated biochemical parameters in the skin and muscle of Pelophylax kl. esculentus from Lake Ludaš (Serbia) by comparing frogs that live on the northern bloom side (BS) of the lake with those that inhabit the southern no-bloom side (NBS). A higher protein carbonylation level and lower antioxidant defense system capability in the skin of frogs living in conditions of the cyanobacterial bloom were observed. Inhibition of glutathione-dependent machinery was the major mechanism responsible for the induction of cyanobacterial bloom-mediated oxidative stress in frog skin. On the other hand, the detected higher ability of muscle to overcome bloom prooxidant toxicity was linked to a higher efficiency of the biotransformation system through glutathione-S-transferase activity and/or was the consequence of indirect exposure of the tissue to the bloom. Our results have also revealed that the cyanobacterial bloom conditions induced the cholinergic neurotransmitter system in both tissues. This study provides a better understanding of the ecotoxicological impact of the MC producing cyanobacterial bloom on frogs in situ. However, further investigations of the complex mechanism involved in cyanobacterial bloom toxicity in real environmental conditions are required.
PB  - Elsevier B.V.
T2  - Aquatic Toxicology
T1  - Biochemical parameters in skin and muscle of Pelophylax kl. esculentus frogs: Influence of a cyanobacterial bloom in situ
VL  - 220
DO  - 10.1016/j.aquatox.2019.105399
SP  - 105399
ER  - 

@article{
author = "Gavrilović, Branka and Prokić, Marko and Petrović, Tamara and Despotović, Svetlana and Radovanović, Tijana and Krizmanić, Imre and Ćirić, Miloš and Gavrić, Jelena",
year = "2020",
abstract = "There is little information in scientific literature as to how conditions created by a microcystin (MC) producing cyanobacterial bloom affect the oxidant/antioxidant, biotransformation and neurotoxicity parameters in adult frogs in situ. We investigated biochemical parameters in the skin and muscle of Pelophylax kl. esculentus from Lake Ludaš (Serbia) by comparing frogs that live on the northern bloom side (BS) of the lake with those that inhabit the southern no-bloom side (NBS). A higher protein carbonylation level and lower antioxidant defense system capability in the skin of frogs living in conditions of the cyanobacterial bloom were observed. Inhibition of glutathione-dependent machinery was the major mechanism responsible for the induction of cyanobacterial bloom-mediated oxidative stress in frog skin. On the other hand, the detected higher ability of muscle to overcome bloom prooxidant toxicity was linked to a higher efficiency of the biotransformation system through glutathione-S-transferase activity and/or was the consequence of indirect exposure of the tissue to the bloom. Our results have also revealed that the cyanobacterial bloom conditions induced the cholinergic neurotransmitter system in both tissues. This study provides a better understanding of the ecotoxicological impact of the MC producing cyanobacterial bloom on frogs in situ. However, further investigations of the complex mechanism involved in cyanobacterial bloom toxicity in real environmental conditions are required.",
publisher = "Elsevier B.V.",
journal = "Aquatic Toxicology",
title = "Biochemical parameters in skin and muscle of Pelophylax kl. esculentus frogs: Influence of a cyanobacterial bloom in situ",
volume = "220",
doi = "10.1016/j.aquatox.2019.105399",
pages = "105399"
}

Gavrilović, B., Prokić, M., Petrović, T., Despotović, S., Radovanović, T., Krizmanić, I., Ćirić, M.,& Gavrić, J.. (2020). Biochemical parameters in skin and muscle of Pelophylax kl. esculentus frogs: Influence of a cyanobacterial bloom in situ. in Aquatic Toxicology
Elsevier B.V.., 220, 105399.
https://doi.org/10.1016/j.aquatox.2019.105399

Gavrilović B, Prokić M, Petrović T, Despotović S, Radovanović T, Krizmanić I, Ćirić M, Gavrić J. Biochemical parameters in skin and muscle of Pelophylax kl. esculentus frogs: Influence of a cyanobacterial bloom in situ. in Aquatic Toxicology. 2020;220:105399.
doi:10.1016/j.aquatox.2019.105399 .

Gavrilović, Branka, Prokić, Marko, Petrović, Tamara, Despotović, Svetlana, Radovanović, Tijana, Krizmanić, Imre, Ćirić, Miloš, Gavrić, Jelena, "Biochemical parameters in skin and muscle of Pelophylax kl. esculentus frogs: Influence of a cyanobacterial bloom in situ" in Aquatic Toxicology, 220 (2020):105399,
https://doi.org/10.1016/j.aquatox.2019.105399 . .

TY  - JOUR
AU  - Prokić, Marko
AU  - Gavrić, Jelena
AU  - Petrović, Tamara
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Krizmanić, Imre
AU  - Pavlović, Slađan
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S109564331930090X?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3338
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3409
AB  - During life, anuran individuals undergo drastic changes in the course of transition from aquatic to terrestrial habitat, when they are faced with metabolically demanding processes (growth, responses to developmental pressures), which result in increased production of reactive oxygen species (ROS), signaling molecules involved in development that can induce oxidative damage and stress. This situation can be further complicated by environmental influences. The aim of this study was to investigate oxidative stress parameters in naturally developing Pelophylax esculentus complex frogs during four developmental periods: premetamorphosis, prometamorphosis, metamorphic climax and juvenile stage, in order to examine changes in the response of the antioxidative system (AOS) and oxidative damage during the transition from aquatic to terrestrial life. Results show that ontogenetic shifts in anurans are accompanied by different levels of damage and AOS responses, which vary from the increased first-line enzymatic activities during the early period of development (premetamorphosis), through increased changes in the non-enzymatic complement during the metamorphic climax, to changes in both the enzymatic and non-enzymatic components observed in juvenile individuals. Premetamorphic individuals and individuals in metamorphosis displayed higher levels of lipid peroxidation, indicating that direct exposure to the environment for the first time and the modulation of organs are the most susceptible stages for oxidative damage. On the other hand, lower oxidative damage in juveniles points to the ability of their AOS to efficiently respond to challenges of the terrestrial environment. This study highlights the importance of ROS and the AOS of anurans in response to different developmental and/or environmental pressures that individuals face.
T2  - Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
T1  - Oxidative stress in Pelophylax esculentus complex frogs in the wild during transition from aquatic to terrestrial life
IS  - Part A
VL  - 234
DO  - 10.1016/J.CBPA.2019.05.004
SP  - 98
EP  - 105
ER  - 

@article{
author = "Prokić, Marko and Gavrić, Jelena and Petrović, Tamara and Despotović, Svetlana and Gavrilović, Branka and Radovanović, Tijana and Krizmanić, Imre and Pavlović, Slađan",
year = "2019",
abstract = "During life, anuran individuals undergo drastic changes in the course of transition from aquatic to terrestrial habitat, when they are faced with metabolically demanding processes (growth, responses to developmental pressures), which result in increased production of reactive oxygen species (ROS), signaling molecules involved in development that can induce oxidative damage and stress. This situation can be further complicated by environmental influences. The aim of this study was to investigate oxidative stress parameters in naturally developing Pelophylax esculentus complex frogs during four developmental periods: premetamorphosis, prometamorphosis, metamorphic climax and juvenile stage, in order to examine changes in the response of the antioxidative system (AOS) and oxidative damage during the transition from aquatic to terrestrial life. Results show that ontogenetic shifts in anurans are accompanied by different levels of damage and AOS responses, which vary from the increased first-line enzymatic activities during the early period of development (premetamorphosis), through increased changes in the non-enzymatic complement during the metamorphic climax, to changes in both the enzymatic and non-enzymatic components observed in juvenile individuals. Premetamorphic individuals and individuals in metamorphosis displayed higher levels of lipid peroxidation, indicating that direct exposure to the environment for the first time and the modulation of organs are the most susceptible stages for oxidative damage. On the other hand, lower oxidative damage in juveniles points to the ability of their AOS to efficiently respond to challenges of the terrestrial environment. This study highlights the importance of ROS and the AOS of anurans in response to different developmental and/or environmental pressures that individuals face.",
journal = "Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology",
title = "Oxidative stress in Pelophylax esculentus complex frogs in the wild during transition from aquatic to terrestrial life",
number = "Part A",
volume = "234",
doi = "10.1016/J.CBPA.2019.05.004",
pages = "98-105"
}

Prokić, M., Gavrić, J., Petrović, T., Despotović, S., Gavrilović, B., Radovanović, T., Krizmanić, I.,& Pavlović, S.. (2019). Oxidative stress in Pelophylax esculentus complex frogs in the wild during transition from aquatic to terrestrial life. in Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 234(Part A), 98-105.
https://doi.org/10.1016/J.CBPA.2019.05.004

Prokić M, Gavrić J, Petrović T, Despotović S, Gavrilović B, Radovanović T, Krizmanić I, Pavlović S. Oxidative stress in Pelophylax esculentus complex frogs in the wild during transition from aquatic to terrestrial life. in Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. 2019;234(Part A):98-105.
doi:10.1016/J.CBPA.2019.05.004 .

Prokić, Marko, Gavrić, Jelena, Petrović, Tamara, Despotović, Svetlana, Gavrilović, Branka, Radovanović, Tijana, Krizmanić, Imre, Pavlović, Slađan, "Oxidative stress in Pelophylax esculentus complex frogs in the wild during transition from aquatic to terrestrial life" in Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 234, no. Part A (2019):98-105,
https://doi.org/10.1016/J.CBPA.2019.05.004 . .

TY  - JOUR
AU  - Prokić, Marko
AU  - Gavrić, Jelena
AU  - Petrović, Tamara
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Krizmanić, Imre
AU  - Pavlović, Slađan
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S109564331930090X?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3338
AB  - During life, anuran individuals undergo drastic changes in the course of transition from aquatic to terrestrial habitat, when they are faced with metabolically demanding processes (growth, responses to developmental pressures), which result in increased production of reactive oxygen species (ROS), signaling molecules involved in development that can induce oxidative damage and stress. This situation can be further complicated by environmental influences. The aim of this study was to investigate oxidative stress parameters in naturally developing Pelophylax esculentus complex frogs during four developmental periods: premetamorphosis, prometamorphosis, metamorphic climax and juvenile stage, in order to examine changes in the response of the antioxidative system (AOS) and oxidative damage during the transition from aquatic to terrestrial life. Results show that ontogenetic shifts in anurans are accompanied by different levels of damage and AOS responses, which vary from the increased first-line enzymatic activities during the early period of development (premetamorphosis), through increased changes in the non-enzymatic complement during the metamorphic climax, to changes in both the enzymatic and non-enzymatic components observed in juvenile individuals. Premetamorphic individuals and individuals in metamorphosis displayed higher levels of lipid peroxidation, indicating that direct exposure to the environment for the first time and the modulation of organs are the most susceptible stages for oxidative damage. On the other hand, lower oxidative damage in juveniles points to the ability of their AOS to efficiently respond to challenges of the terrestrial environment. This study highlights the importance of ROS and the AOS of anurans in response to different developmental and/or environmental pressures that individuals face.
T2  - Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
T1  - Oxidative stress in Pelophylax esculentus complex frogs in the wild during transition from aquatic to terrestrial life
DO  - 10.1016/J.CBPA.2019.05.004
ER  - 

@article{
author = "Prokić, Marko and Gavrić, Jelena and Petrović, Tamara and Despotović, Svetlana and Gavrilović, Branka and Radovanović, Tijana and Krizmanić, Imre and Pavlović, Slađan",
year = "2019",
abstract = "During life, anuran individuals undergo drastic changes in the course of transition from aquatic to terrestrial habitat, when they are faced with metabolically demanding processes (growth, responses to developmental pressures), which result in increased production of reactive oxygen species (ROS), signaling molecules involved in development that can induce oxidative damage and stress. This situation can be further complicated by environmental influences. The aim of this study was to investigate oxidative stress parameters in naturally developing Pelophylax esculentus complex frogs during four developmental periods: premetamorphosis, prometamorphosis, metamorphic climax and juvenile stage, in order to examine changes in the response of the antioxidative system (AOS) and oxidative damage during the transition from aquatic to terrestrial life. Results show that ontogenetic shifts in anurans are accompanied by different levels of damage and AOS responses, which vary from the increased first-line enzymatic activities during the early period of development (premetamorphosis), through increased changes in the non-enzymatic complement during the metamorphic climax, to changes in both the enzymatic and non-enzymatic components observed in juvenile individuals. Premetamorphic individuals and individuals in metamorphosis displayed higher levels of lipid peroxidation, indicating that direct exposure to the environment for the first time and the modulation of organs are the most susceptible stages for oxidative damage. On the other hand, lower oxidative damage in juveniles points to the ability of their AOS to efficiently respond to challenges of the terrestrial environment. This study highlights the importance of ROS and the AOS of anurans in response to different developmental and/or environmental pressures that individuals face.",
journal = "Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology",
title = "Oxidative stress in Pelophylax esculentus complex frogs in the wild during transition from aquatic to terrestrial life",
doi = "10.1016/J.CBPA.2019.05.004"
}

Prokić, M., Gavrić, J., Petrović, T., Despotović, S., Gavrilović, B., Radovanović, T., Krizmanić, I.,& Pavlović, S.. (2019). Oxidative stress in Pelophylax esculentus complex frogs in the wild during transition from aquatic to terrestrial life. in Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology.
https://doi.org/10.1016/J.CBPA.2019.05.004

Prokić M, Gavrić J, Petrović T, Despotović S, Gavrilović B, Radovanović T, Krizmanić I, Pavlović S. Oxidative stress in Pelophylax esculentus complex frogs in the wild during transition from aquatic to terrestrial life. in Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. 2019;.
doi:10.1016/J.CBPA.2019.05.004 .

Prokić, Marko, Gavrić, Jelena, Petrović, Tamara, Despotović, Svetlana, Gavrilović, Branka, Radovanović, Tijana, Krizmanić, Imre, Pavlović, Slađan, "Oxidative stress in Pelophylax esculentus complex frogs in the wild during transition from aquatic to terrestrial life" in Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology (2019),
https://doi.org/10.1016/J.CBPA.2019.05.004 . .