TY  - JOUR
AU  - Dinić, Jelena
AU  - Efferth, Thomas
AU  - García-Sosa, Alfonso T.
AU  - Grahovac, Jelena
AU  - Padrón, José M.
AU  - Pajeva, Ilza
AU  - Rizzolio, Flavio
AU  - Saponara, Simona
AU  - Spengler, Gabriella
AU  - Tsakovska, Ivanka
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3758
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3767
AB  - Overcoming multidrug resistance represents a major challenge for cancer treatment. In the search for new chemotherapeutics to treat malignant diseases, drug repurposing gained a tremendous interest during the past years. Repositioning candidates have often emerged through several stages of clinical drug development, and may even be marketed, thus attracting the attention and interest of pharmaceutical companies as well as regulatory agencies. Typically, drug repositioning has been serendipitous, using undesired side effects of small molecule drugs to exploit new disease indications. As bioinformatics gain increasing popularity as an integral component of drug discovery, more rational approaches are needed. Herein, we show some practical examples of in silico approaches such as pharmacophore modelling, as well as pharmacophore- and docking-based virtual screening for a fast and cost-effective repurposing of small molecule drugs against multidrug resistant cancers. We provide a timely and comprehensive overview of compounds with considerable potential to be repositioned for cancer therapeutics. These drugs are from diverse chemotherapeutic classes. We emphasize the scope and limitations of anthelmintics, antibiotics, antifungals, antivirals, antimalarials, antihypertensives, psychopharmaceuticals and antidiabetics that have shown extensive immunomodulatory, antiproliferative, pro-apoptotic, and antimetastatic potential. These drugs, either used alone or in combination with existing anticancer chemotherapeutics, represent strong candidates to prevent or overcome drug resistance. We particularly focus on outcomes and future perspectives of drug repositioning for the treatment of multidrug resistant tumors and discuss current possibilities and limitations of preclinical and clinical investigations.
PB  - Churchill Livingstone
T2  - Drug Resistance Updates
T1  - Repurposing old drugs to fight multidrug resistant cancers
VL  - 52
DO  - 10.1016/j.drup.2020.100713
SP  - 100713
ER  - 

@article{
author = "Dinić, Jelena and Efferth, Thomas and García-Sosa, Alfonso T. and Grahovac, Jelena and Padrón, José M. and Pajeva, Ilza and Rizzolio, Flavio and Saponara, Simona and Spengler, Gabriella and Tsakovska, Ivanka",
year = "2020",
abstract = "Overcoming multidrug resistance represents a major challenge for cancer treatment. In the search for new chemotherapeutics to treat malignant diseases, drug repurposing gained a tremendous interest during the past years. Repositioning candidates have often emerged through several stages of clinical drug development, and may even be marketed, thus attracting the attention and interest of pharmaceutical companies as well as regulatory agencies. Typically, drug repositioning has been serendipitous, using undesired side effects of small molecule drugs to exploit new disease indications. As bioinformatics gain increasing popularity as an integral component of drug discovery, more rational approaches are needed. Herein, we show some practical examples of in silico approaches such as pharmacophore modelling, as well as pharmacophore- and docking-based virtual screening for a fast and cost-effective repurposing of small molecule drugs against multidrug resistant cancers. We provide a timely and comprehensive overview of compounds with considerable potential to be repositioned for cancer therapeutics. These drugs are from diverse chemotherapeutic classes. We emphasize the scope and limitations of anthelmintics, antibiotics, antifungals, antivirals, antimalarials, antihypertensives, psychopharmaceuticals and antidiabetics that have shown extensive immunomodulatory, antiproliferative, pro-apoptotic, and antimetastatic potential. These drugs, either used alone or in combination with existing anticancer chemotherapeutics, represent strong candidates to prevent or overcome drug resistance. We particularly focus on outcomes and future perspectives of drug repositioning for the treatment of multidrug resistant tumors and discuss current possibilities and limitations of preclinical and clinical investigations.",
publisher = "Churchill Livingstone",
journal = "Drug Resistance Updates",
title = "Repurposing old drugs to fight multidrug resistant cancers",
volume = "52",
doi = "10.1016/j.drup.2020.100713",
pages = "100713"
}

Dinić, J., Efferth, T., García-Sosa, A. T., Grahovac, J., Padrón, J. M., Pajeva, I., Rizzolio, F., Saponara, S., Spengler, G.,& Tsakovska, I.. (2020). Repurposing old drugs to fight multidrug resistant cancers. in Drug Resistance Updates
Churchill Livingstone., 52, 100713.
https://doi.org/10.1016/j.drup.2020.100713

Dinić J, Efferth T, García-Sosa AT, Grahovac J, Padrón JM, Pajeva I, Rizzolio F, Saponara S, Spengler G, Tsakovska I. Repurposing old drugs to fight multidrug resistant cancers. in Drug Resistance Updates. 2020;52:100713.
doi:10.1016/j.drup.2020.100713 .

Dinić, Jelena, Efferth, Thomas, García-Sosa, Alfonso T., Grahovac, Jelena, Padrón, José M., Pajeva, Ilza, Rizzolio, Flavio, Saponara, Simona, Spengler, Gabriella, Tsakovska, Ivanka, "Repurposing old drugs to fight multidrug resistant cancers" in Drug Resistance Updates, 52 (2020):100713,
https://doi.org/10.1016/j.drup.2020.100713 . .

TY  - JOUR
AU  - Dinić, Jelena
AU  - Efferth, Thomas
AU  - García-Sosa, Alfonso T.
AU  - Grahovac, Jelena
AU  - Padrón, José M.
AU  - Pajeva, Ilza
AU  - Rizzolio, Flavio
AU  - Saponara, Simona
AU  - Spengler, Gabriella
AU  - Tsakovska, Ivanka
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3758
AB  - Overcoming multidrug resistance represents a major challenge for cancer treatment. In the search for new chemotherapeutics to treat malignant diseases, drug repurposing gained a tremendous interest during the past years. Repositioning candidates have often emerged through several stages of clinical drug development, and may even be marketed, thus attracting the attention and interest of pharmaceutical companies as well as regulatory agencies. Typically, drug repositioning has been serendipitous, using undesired side effects of small molecule drugs to exploit new disease indications. As bioinformatics gain increasing popularity as an integral component of drug discovery, more rational approaches are needed. Herein, we show some practical examples of in silico approaches such as pharmacophore modelling, as well as pharmacophore- and docking-based virtual screening for a fast and cost-effective repurposing of small molecule drugs against multidrug resistant cancers. We provide a timely and comprehensive overview of compounds with considerable potential to be repositioned for cancer therapeutics. These drugs are from diverse chemotherapeutic classes. We emphasize the scope and limitations of anthelmintics, antibiotics, antifungals, antivirals, antimalarials, antihypertensives, psychopharmaceuticals and antidiabetics that have shown extensive immunomodulatory, antiproliferative, pro-apoptotic, and antimetastatic potential. These drugs, either used alone or in combination with existing anticancer chemotherapeutics, represent strong candidates to prevent or overcome drug resistance. We particularly focus on outcomes and future perspectives of drug repositioning for the treatment of multidrug resistant tumors and discuss current possibilities and limitations of preclinical and clinical investigations.
PB  - Churchill Livingstone
T2  - Drug Resistance Updates
T1  - Repurposing old drugs to fight multidrug resistant cancers
VL  - 52
DO  - 10.1016/j.drup.2020.100713
SP  - 100713
ER  - 

@article{
author = "Dinić, Jelena and Efferth, Thomas and García-Sosa, Alfonso T. and Grahovac, Jelena and Padrón, José M. and Pajeva, Ilza and Rizzolio, Flavio and Saponara, Simona and Spengler, Gabriella and Tsakovska, Ivanka",
year = "2020",
abstract = "Overcoming multidrug resistance represents a major challenge for cancer treatment. In the search for new chemotherapeutics to treat malignant diseases, drug repurposing gained a tremendous interest during the past years. Repositioning candidates have often emerged through several stages of clinical drug development, and may even be marketed, thus attracting the attention and interest of pharmaceutical companies as well as regulatory agencies. Typically, drug repositioning has been serendipitous, using undesired side effects of small molecule drugs to exploit new disease indications. As bioinformatics gain increasing popularity as an integral component of drug discovery, more rational approaches are needed. Herein, we show some practical examples of in silico approaches such as pharmacophore modelling, as well as pharmacophore- and docking-based virtual screening for a fast and cost-effective repurposing of small molecule drugs against multidrug resistant cancers. We provide a timely and comprehensive overview of compounds with considerable potential to be repositioned for cancer therapeutics. These drugs are from diverse chemotherapeutic classes. We emphasize the scope and limitations of anthelmintics, antibiotics, antifungals, antivirals, antimalarials, antihypertensives, psychopharmaceuticals and antidiabetics that have shown extensive immunomodulatory, antiproliferative, pro-apoptotic, and antimetastatic potential. These drugs, either used alone or in combination with existing anticancer chemotherapeutics, represent strong candidates to prevent or overcome drug resistance. We particularly focus on outcomes and future perspectives of drug repositioning for the treatment of multidrug resistant tumors and discuss current possibilities and limitations of preclinical and clinical investigations.",
publisher = "Churchill Livingstone",
journal = "Drug Resistance Updates",
title = "Repurposing old drugs to fight multidrug resistant cancers",
volume = "52",
doi = "10.1016/j.drup.2020.100713",
pages = "100713"
}

Dinić, J., Efferth, T., García-Sosa, A. T., Grahovac, J., Padrón, J. M., Pajeva, I., Rizzolio, F., Saponara, S., Spengler, G.,& Tsakovska, I.. (2020). Repurposing old drugs to fight multidrug resistant cancers. in Drug Resistance Updates
Churchill Livingstone., 52, 100713.
https://doi.org/10.1016/j.drup.2020.100713

Dinić J, Efferth T, García-Sosa AT, Grahovac J, Padrón JM, Pajeva I, Rizzolio F, Saponara S, Spengler G, Tsakovska I. Repurposing old drugs to fight multidrug resistant cancers. in Drug Resistance Updates. 2020;52:100713.
doi:10.1016/j.drup.2020.100713 .

Dinić, Jelena, Efferth, Thomas, García-Sosa, Alfonso T., Grahovac, Jelena, Padrón, José M., Pajeva, Ilza, Rizzolio, Flavio, Saponara, Simona, Spengler, Gabriella, Tsakovska, Ivanka, "Repurposing old drugs to fight multidrug resistant cancers" in Drug Resistance Updates, 52 (2020):100713,
https://doi.org/10.1016/j.drup.2020.100713 . .

TY  - JOUR
AU  - Devrnja, Nina
AU  - Anđelković, Boban
AU  - Aranđelović, Sandra
AU  - Radulović, Siniša
AU  - Soković, Marina
AU  - Krstić Milošević, Dijana
AU  - Ristić, Mihailo
AU  - Ćalić, Dušica
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0254629916339928
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2721
AB  - Chemical composition of essential oil (EO) and methanol extracts (MEs) from different parts of Tanacetum vulgare L. plant was analyzed and investigated for potential biological activities and correlated with the main constituents detected in EO and MEs. The EO was characterized by a high content of oxygenated monoterpenes with trans-chrysanthenyl acetate as major compound. All MEs were characterized by neochlorogenic, 3,5-O-dicaffeoylquinic and caffeoylquinic acids. High phenolic content in MEs correlated to high antioxidant capacity, especially for roots. Tansy EO showed strong activity against the most of tested fungi and was efficient as bifonazole and ketoconazole, while the most sensitive bacteria were Gram-negative E. coli and E. cloacae. All MEs showed fungistatic and fungicidal effects against all of the eight tested fungi, but the antimicrobial activity was higher against Gram-positive bacteria. Additionally, shown for the first time, MEs of leaves and flowers exhibited a strong antiproliferative effect on human cervical adenocarcinoma (HeLa) cells, causing cell shrinkage and detachment. In the present study, the Tansy extracts and essential oil with low thujone content can provide a very promising and effective alternative in the field of antimicrobial applications and food preservation. The Tansy MEs possess a high antioxidant potential with phenolic acids being a major radical scavenging contributor with the proved antiproliferative activity to HeLa cells.
T2  - South African Journal of Botany
T1  - Comparative studies on the antimicrobial and cytotoxic activities of Tanacetum vulgare L. essential oil and methanol extracts
VL  - 111
DO  - 10.1016/j.sajb.2017.03.028
SP  - 212
EP  - 221
ER  - 

@article{
author = "Devrnja, Nina and Anđelković, Boban and Aranđelović, Sandra and Radulović, Siniša and Soković, Marina and Krstić Milošević, Dijana and Ristić, Mihailo and Ćalić, Dušica",
year = "2017",
abstract = "Chemical composition of essential oil (EO) and methanol extracts (MEs) from different parts of Tanacetum vulgare L. plant was analyzed and investigated for potential biological activities and correlated with the main constituents detected in EO and MEs. The EO was characterized by a high content of oxygenated monoterpenes with trans-chrysanthenyl acetate as major compound. All MEs were characterized by neochlorogenic, 3,5-O-dicaffeoylquinic and caffeoylquinic acids. High phenolic content in MEs correlated to high antioxidant capacity, especially for roots. Tansy EO showed strong activity against the most of tested fungi and was efficient as bifonazole and ketoconazole, while the most sensitive bacteria were Gram-negative E. coli and E. cloacae. All MEs showed fungistatic and fungicidal effects against all of the eight tested fungi, but the antimicrobial activity was higher against Gram-positive bacteria. Additionally, shown for the first time, MEs of leaves and flowers exhibited a strong antiproliferative effect on human cervical adenocarcinoma (HeLa) cells, causing cell shrinkage and detachment. In the present study, the Tansy extracts and essential oil with low thujone content can provide a very promising and effective alternative in the field of antimicrobial applications and food preservation. The Tansy MEs possess a high antioxidant potential with phenolic acids being a major radical scavenging contributor with the proved antiproliferative activity to HeLa cells.",
journal = "South African Journal of Botany",
title = "Comparative studies on the antimicrobial and cytotoxic activities of Tanacetum vulgare L. essential oil and methanol extracts",
volume = "111",
doi = "10.1016/j.sajb.2017.03.028",
pages = "212-221"
}

Devrnja, N., Anđelković, B., Aranđelović, S., Radulović, S., Soković, M., Krstić Milošević, D., Ristić, M.,& Ćalić, D.. (2017). Comparative studies on the antimicrobial and cytotoxic activities of Tanacetum vulgare L. essential oil and methanol extracts. in South African Journal of Botany, 111, 212-221.
https://doi.org/10.1016/j.sajb.2017.03.028

Devrnja N, Anđelković B, Aranđelović S, Radulović S, Soković M, Krstić Milošević D, Ristić M, Ćalić D. Comparative studies on the antimicrobial and cytotoxic activities of Tanacetum vulgare L. essential oil and methanol extracts. in South African Journal of Botany. 2017;111:212-221.
doi:10.1016/j.sajb.2017.03.028 .

Devrnja, Nina, Anđelković, Boban, Aranđelović, Sandra, Radulović, Siniša, Soković, Marina, Krstić Milošević, Dijana, Ristić, Mihailo, Ćalić, Dušica, "Comparative studies on the antimicrobial and cytotoxic activities of Tanacetum vulgare L. essential oil and methanol extracts" in South African Journal of Botany, 111 (2017):212-221,
https://doi.org/10.1016/j.sajb.2017.03.028 . .

TY  - JOUR
AU  - Nikolić, Miloš
AU  - Jovanović, Katarina
AU  - Marković, Tatjana
AU  - Marković, Dejan
AU  - Gligorijević, Nevenka
AU  - Radulović, Siniša
AU  - Kostić, Marina
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
PY  - 2017
UR  - http://doi.wiley.com/10.1111/jphp.12792
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2875
AB  - Objectives: The chemical composition, antimicrobial and synergistic effect, and cytotoxic activity of Citrus limon (lemon), Piper nigrum (green pepper) and Melaleuca alternifoila (tea tree) essential oils (EOs) were investigated. Methods: Chemical analyses of essential oils were tested by GC-FID and GC-MS spectroscopy. The antimicrobial activity assay was conducted using microdilution method against several oral bacteria and Candida spp. originating from the humans with oral disorders. The synergistic antimicrobial activity was evaluated using checkerboard method. The cytotoxicity evaluation of EOs was assessed using MTT test. Key findings: Limonene (37.5%) and β-pinene (17.9%) were the major compounds in C. limon oil, β-pinene (34.4%), δ-3-carene (19.7%), limonene (18.7%) and α-pinene (10.4%) in P. nigrum oil and terpinen-4-ol (38.6%) and γ-terpinene (21.7%) in M. alternifolia oil. The broad-spectrum antimicrobial activity was achieved by tested three EOs, with C. limon oil being the strongest against bacteria and M. alternifolia oil strongest against fungi. The EOs demonstrated synergism; their combined application revealed an increase in antimicrobial activity. All tested essential oils showed lower cytotoxic activity in comparison with the positive control, and the obtained results confirmed a dose-dependent activity. Conclusions: The results of this study encourage use of tested EOs in development of a novel agent intended for prevention or therapy of corresponding oral disorders.
T2  - Journal of Pharmacy and Pharmacology
T1  - Antimicrobial synergism and cytotoxic properties of Citrus limon L., Piper nigrum L. and Melaleuca alternifolia (Maiden and Betche) Cheel essential oils
IS  - 11
VL  - 69
DO  - 10.1111/jphp.12792
SP  - 1606
EP  - 1614
ER  - 

@article{
author = "Nikolić, Miloš and Jovanović, Katarina and Marković, Tatjana and Marković, Dejan and Gligorijević, Nevenka and Radulović, Siniša and Kostić, Marina and Glamočlija, Jasmina and Soković, Marina",
year = "2017",
abstract = "Objectives: The chemical composition, antimicrobial and synergistic effect, and cytotoxic activity of Citrus limon (lemon), Piper nigrum (green pepper) and Melaleuca alternifoila (tea tree) essential oils (EOs) were investigated. Methods: Chemical analyses of essential oils were tested by GC-FID and GC-MS spectroscopy. The antimicrobial activity assay was conducted using microdilution method against several oral bacteria and Candida spp. originating from the humans with oral disorders. The synergistic antimicrobial activity was evaluated using checkerboard method. The cytotoxicity evaluation of EOs was assessed using MTT test. Key findings: Limonene (37.5%) and β-pinene (17.9%) were the major compounds in C. limon oil, β-pinene (34.4%), δ-3-carene (19.7%), limonene (18.7%) and α-pinene (10.4%) in P. nigrum oil and terpinen-4-ol (38.6%) and γ-terpinene (21.7%) in M. alternifolia oil. The broad-spectrum antimicrobial activity was achieved by tested three EOs, with C. limon oil being the strongest against bacteria and M. alternifolia oil strongest against fungi. The EOs demonstrated synergism; their combined application revealed an increase in antimicrobial activity. All tested essential oils showed lower cytotoxic activity in comparison with the positive control, and the obtained results confirmed a dose-dependent activity. Conclusions: The results of this study encourage use of tested EOs in development of a novel agent intended for prevention or therapy of corresponding oral disorders.",
journal = "Journal of Pharmacy and Pharmacology",
title = "Antimicrobial synergism and cytotoxic properties of Citrus limon L., Piper nigrum L. and Melaleuca alternifolia (Maiden and Betche) Cheel essential oils",
number = "11",
volume = "69",
doi = "10.1111/jphp.12792",
pages = "1606-1614"
}

Nikolić, M., Jovanović, K., Marković, T., Marković, D., Gligorijević, N., Radulović, S., Kostić, M., Glamočlija, J.,& Soković, M.. (2017). Antimicrobial synergism and cytotoxic properties of Citrus limon L., Piper nigrum L. and Melaleuca alternifolia (Maiden and Betche) Cheel essential oils. in Journal of Pharmacy and Pharmacology, 69(11), 1606-1614.
https://doi.org/10.1111/jphp.12792

Nikolić M, Jovanović K, Marković T, Marković D, Gligorijević N, Radulović S, Kostić M, Glamočlija J, Soković M. Antimicrobial synergism and cytotoxic properties of Citrus limon L., Piper nigrum L. and Melaleuca alternifolia (Maiden and Betche) Cheel essential oils. in Journal of Pharmacy and Pharmacology. 2017;69(11):1606-1614.
doi:10.1111/jphp.12792 .

Nikolić, Miloš, Jovanović, Katarina, Marković, Tatjana, Marković, Dejan, Gligorijević, Nevenka, Radulović, Siniša, Kostić, Marina, Glamočlija, Jasmina, Soković, Marina, "Antimicrobial synergism and cytotoxic properties of Citrus limon L., Piper nigrum L. and Melaleuca alternifolia (Maiden and Betche) Cheel essential oils" in Journal of Pharmacy and Pharmacology, 69, no. 11 (2017):1606-1614,
https://doi.org/10.1111/jphp.12792 . .

TY  - JOUR
AU  - Skorić, Marijana
AU  - Gligorijević, Nevenka
AU  - Čavić, Milena
AU  - Todorović, Slađana
AU  - Janković, Radmila
AU  - Ristić, Mihailo
AU  - Mišić, Danijela
AU  - Radulović, Siniša
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0926669017301309
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2609
AB  - Nepeta rtanjensis Diklić & Milojević (fam. Lamiaceae) is an endemic, critically endangered plant, protected by the law in Serbia. Various biological activities have been ascribed to its major constituents, nepetalactones. In this study we describe for the first time cytotoxic activity of N. rtanjensis essential oil (EO), obtained from field cultivated plants, which was found to be especially rich in trans,cis-nepetalactone. MTT assays indicated that after 72 h of treatment the EO exhibited cytotoxic activity on investigated cancer cell lines: HeLa, K562, A549, LS-174 and MDA-MB-231. Normal cell line (MRC-5) was the least sensitive to the treatment and IC50 value for this cell line was not reached within the tested range of EO concentrations (up to 0.1 μL/mL). Analysis of morphological changes of treated cells confirmed the higher sensitivity of tumor cells than normal cells to the tested EO. Application of N. rtanjensis EO resulted in the appearance of morphological changes in tested cancer cell lines characteristic for apoptotic cell death, and induced perturbations of the cell cycle of HeLa cells. In addition, upregulation of Bax and p53, and downregulation of Bcl-2, and Skp2 genes, involved in apoptotic signalling cascades, confirmed an apoptosis-inducing effect of N. rtanjensis EO on HeLa cells. Presented results highlighted the potential of N. rtanjensis EO in anticancer therapy.
T2  - Industrial Crops and Products
T1  - Cytotoxic activity of Nepeta rtanjensis Diklić & Milojević essential oil and its mode of action
VL  - 100
DO  - 10.1016/j.indcrop.2017.02.027
SP  - 163
EP  - 170
ER  - 

@article{
author = "Skorić, Marijana and Gligorijević, Nevenka and Čavić, Milena and Todorović, Slađana and Janković, Radmila and Ristić, Mihailo and Mišić, Danijela and Radulović, Siniša",
year = "2017",
abstract = "Nepeta rtanjensis Diklić & Milojević (fam. Lamiaceae) is an endemic, critically endangered plant, protected by the law in Serbia. Various biological activities have been ascribed to its major constituents, nepetalactones. In this study we describe for the first time cytotoxic activity of N. rtanjensis essential oil (EO), obtained from field cultivated plants, which was found to be especially rich in trans,cis-nepetalactone. MTT assays indicated that after 72 h of treatment the EO exhibited cytotoxic activity on investigated cancer cell lines: HeLa, K562, A549, LS-174 and MDA-MB-231. Normal cell line (MRC-5) was the least sensitive to the treatment and IC50 value for this cell line was not reached within the tested range of EO concentrations (up to 0.1 μL/mL). Analysis of morphological changes of treated cells confirmed the higher sensitivity of tumor cells than normal cells to the tested EO. Application of N. rtanjensis EO resulted in the appearance of morphological changes in tested cancer cell lines characteristic for apoptotic cell death, and induced perturbations of the cell cycle of HeLa cells. In addition, upregulation of Bax and p53, and downregulation of Bcl-2, and Skp2 genes, involved in apoptotic signalling cascades, confirmed an apoptosis-inducing effect of N. rtanjensis EO on HeLa cells. Presented results highlighted the potential of N. rtanjensis EO in anticancer therapy.",
journal = "Industrial Crops and Products",
title = "Cytotoxic activity of Nepeta rtanjensis Diklić & Milojević essential oil and its mode of action",
volume = "100",
doi = "10.1016/j.indcrop.2017.02.027",
pages = "163-170"
}

Skorić, M., Gligorijević, N., Čavić, M., Todorović, S., Janković, R., Ristić, M., Mišić, D.,& Radulović, S.. (2017). Cytotoxic activity of Nepeta rtanjensis Diklić & Milojević essential oil and its mode of action. in Industrial Crops and Products, 100, 163-170.
https://doi.org/10.1016/j.indcrop.2017.02.027

Skorić M, Gligorijević N, Čavić M, Todorović S, Janković R, Ristić M, Mišić D, Radulović S. Cytotoxic activity of Nepeta rtanjensis Diklić & Milojević essential oil and its mode of action. in Industrial Crops and Products. 2017;100:163-170.
doi:10.1016/j.indcrop.2017.02.027 .

Skorić, Marijana, Gligorijević, Nevenka, Čavić, Milena, Todorović, Slađana, Janković, Radmila, Ristić, Mihailo, Mišić, Danijela, Radulović, Siniša, "Cytotoxic activity of Nepeta rtanjensis Diklić & Milojević essential oil and its mode of action" in Industrial Crops and Products, 100 (2017):163-170,
https://doi.org/10.1016/j.indcrop.2017.02.027 . .

TY  - CONF
AU  - Marković, Marija
AU  - Skorić, Marijana
AU  - Živković, Suzana
AU  - Jadranin, Milka
AU  - Dragićević, Milan
AU  - Tešević, Vele
AU  - Gligorijević, Nevenka
AU  - Janković, Radmila
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Todorović, Slađana
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6184
AB  - Inula oculus-christi L. is a perennial species distributed in Asia, Central Europe and the Balkan Peninsula. In this study a potential cytotoxic and antimicrobial activity of ethanol extracts obtained from different parts of I. oculus-christi plants was investigated. Cytotoxic activity of extracts was determined on three tumor cell lines (HeLa, FemX and LS-174) by MTT assay. The extracts of leaves and flowers showed significant cytotoxic activity against all investigated cell lines, while rhizome, root and trunk extracts had no cytotoxic activity. The IC50 values for flower extract were 68.70 ± 2.79 μg mL-1 for HeLa cells, 88.91 ± 1.66 μg mL-1 for FemX cells and 81.46 ± 8.45 μg mL-1 for LS-174 cells, whereas the IC50 values for leaf extract were 73.69 ± 5.35 μg mL-1 for He-La cells, 71.58 ± 4.25 μg mL-1 for FemX cells and 96.37 ± 4.92 μg mL-1 for LS-174 cells. The observed cytotoxic activity could be attributed to several bioactive sesquiterpenoids and flavonoids which were more abundant in leaf and flower extracts according to LC-DAD/MS non-targeted screening. In the antimicrobial bioassays against eight bacteria and microfungi, all tested extracts exhibited higher antimicrobial activity in comparison with commercial antimicrobial agents used as control. These results indicate the possibility of application of I. oculus-christi extracts as valuable natural products in medicine, agronomy and food industry.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - Cytotoxic and antimicrobial activities of different plant part extracts of Inula oculus-christi
SP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6184
ER  - 

@conference{
author = "Marković, Marija and Skorić, Marijana and Živković, Suzana and Jadranin, Milka and Dragićević, Milan and Tešević, Vele and Gligorijević, Nevenka and Janković, Radmila and Glamočlija, Jasmina and Soković, Marina and Todorović, Slađana",
year = "2015",
abstract = "Inula oculus-christi L. is a perennial species distributed in Asia, Central Europe and the Balkan Peninsula. In this study a potential cytotoxic and antimicrobial activity of ethanol extracts obtained from different parts of I. oculus-christi plants was investigated. Cytotoxic activity of extracts was determined on three tumor cell lines (HeLa, FemX and LS-174) by MTT assay. The extracts of leaves and flowers showed significant cytotoxic activity against all investigated cell lines, while rhizome, root and trunk extracts had no cytotoxic activity. The IC50 values for flower extract were 68.70 ± 2.79 μg mL-1 for HeLa cells, 88.91 ± 1.66 μg mL-1 for FemX cells and 81.46 ± 8.45 μg mL-1 for LS-174 cells, whereas the IC50 values for leaf extract were 73.69 ± 5.35 μg mL-1 for He-La cells, 71.58 ± 4.25 μg mL-1 for FemX cells and 96.37 ± 4.92 μg mL-1 for LS-174 cells. The observed cytotoxic activity could be attributed to several bioactive sesquiterpenoids and flavonoids which were more abundant in leaf and flower extracts according to LC-DAD/MS non-targeted screening. In the antimicrobial bioassays against eight bacteria and microfungi, all tested extracts exhibited higher antimicrobial activity in comparison with commercial antimicrobial agents used as control. These results indicate the possibility of application of I. oculus-christi extracts as valuable natural products in medicine, agronomy and food industry.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "Cytotoxic and antimicrobial activities of different plant part extracts of Inula oculus-christi",
pages = "85",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6184"
}

Marković, M., Skorić, M., Živković, S., Jadranin, M., Dragićević, M., Tešević, V., Gligorijević, N., Janković, R., Glamočlija, J., Soković, M.,& Todorović, S.. (2015). Cytotoxic and antimicrobial activities of different plant part extracts of Inula oculus-christi. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Belgrade: Serbian Plant Physiology Society., 85.
https://hdl.handle.net/21.15107/rcub_ibiss_6184

Marković M, Skorić M, Živković S, Jadranin M, Dragićević M, Tešević V, Gligorijević N, Janković R, Glamočlija J, Soković M, Todorović S. Cytotoxic and antimicrobial activities of different plant part extracts of Inula oculus-christi. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:85.
https://hdl.handle.net/21.15107/rcub_ibiss_6184 .

Marković, Marija, Skorić, Marijana, Živković, Suzana, Jadranin, Milka, Dragićević, Milan, Tešević, Vele, Gligorijević, Nevenka, Janković, Radmila, Glamočlija, Jasmina, Soković, Marina, Todorović, Slađana, "Cytotoxic and antimicrobial activities of different plant part extracts of Inula oculus-christi" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):85,
https://hdl.handle.net/21.15107/rcub_ibiss_6184 .