TY  - JOUR
AU  - Batinić, Petar
AU  - Jovanović, Aleksandra
AU  - Stojković, Dejan
AU  - Zengin, Gokhan
AU  - Cvijetić, Ilija
AU  - Gašić, Uroš
AU  - Čutović, Natalija
AU  - Pešić, Mirjana
AU  - Milinčić, Danijel
AU  - Carević, Tamara
AU  - Marinković, Aleksandar
AU  - Bugarski, Branko
AU  - Marković, Tatjana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6687
AB  - Without being aware of their chemical composition, many cultures have used herbaceous peony roots for medicinal purposes. Modern phytopreparations intended for use in human therapy require specific knowledge about the chemistry of peony roots and their biological activities. In this study, ethanol–water extracts were prepared by maceration and microwave- and ultrasound-assisted extractions (MAE and UAE, respectively) in order to obtain bioactive molecules from the roots of Paeonia tenuifolia L., Paeonia peregrina Mill., and Paeonia officinalis L. wild growing in Serbia. Chemical characterization; polyphenol and flavonoid content; antioxidant, multianti-enzymatic, and antibacterial activities of extracts; and in vitro gastrointestinal digestion (GID) of hot water extracts were performed. The strongest anti-cholinesterase activity was observed in PT extracts. The highest anti-ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical potential was observed in PP extracts, whereas against DPPH (2,2-diphenyl-1-picrylhydrazyl radicals), the best results were achieved with PO extracts. Regarding antibacterial activity, extracts were strongly potent against Bacillus cereus. A molecular docking simulation was conducted to gather insights into the binding affinity and interactions of polyphenols and other Paeonia-specific molecules in the active sites of tested enzymes. In vitro GID of Paeonia teas showed a different recovery and behavior of the individual bioactives, with an increased recovery of methyl gallate and digallate and a decreased recovery of paeoniflorin and its derivatives. PT (Gulenovci) and PP (Pirot) extracts obtained by UAE and M were more efficient in the majority of the bioactivity assays. This study represents an initial step toward the possible application of Paeonia root extracts in pharmacy, medicine, and food technologies.
PB  - Basel: MDPI
T2  - Pharmaceuticals
T1  - Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia
IS  - 4
VL  - 17
DO  - 10.3390/ph17040518
SP  - 518
ER  - 

@article{
author = "Batinić, Petar and Jovanović, Aleksandra and Stojković, Dejan and Zengin, Gokhan and Cvijetić, Ilija and Gašić, Uroš and Čutović, Natalija and Pešić, Mirjana and Milinčić, Danijel and Carević, Tamara and Marinković, Aleksandar and Bugarski, Branko and Marković, Tatjana",
year = "2024",
abstract = "Without being aware of their chemical composition, many cultures have used herbaceous peony roots for medicinal purposes. Modern phytopreparations intended for use in human therapy require specific knowledge about the chemistry of peony roots and their biological activities. In this study, ethanol–water extracts were prepared by maceration and microwave- and ultrasound-assisted extractions (MAE and UAE, respectively) in order to obtain bioactive molecules from the roots of Paeonia tenuifolia L., Paeonia peregrina Mill., and Paeonia officinalis L. wild growing in Serbia. Chemical characterization; polyphenol and flavonoid content; antioxidant, multianti-enzymatic, and antibacterial activities of extracts; and in vitro gastrointestinal digestion (GID) of hot water extracts were performed. The strongest anti-cholinesterase activity was observed in PT extracts. The highest anti-ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical potential was observed in PP extracts, whereas against DPPH (2,2-diphenyl-1-picrylhydrazyl radicals), the best results were achieved with PO extracts. Regarding antibacterial activity, extracts were strongly potent against Bacillus cereus. A molecular docking simulation was conducted to gather insights into the binding affinity and interactions of polyphenols and other Paeonia-specific molecules in the active sites of tested enzymes. In vitro GID of Paeonia teas showed a different recovery and behavior of the individual bioactives, with an increased recovery of methyl gallate and digallate and a decreased recovery of paeoniflorin and its derivatives. PT (Gulenovci) and PP (Pirot) extracts obtained by UAE and M were more efficient in the majority of the bioactivity assays. This study represents an initial step toward the possible application of Paeonia root extracts in pharmacy, medicine, and food technologies.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals",
title = "Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia",
number = "4",
volume = "17",
doi = "10.3390/ph17040518",
pages = "518"
}

Batinić, P., Jovanović, A., Stojković, D., Zengin, G., Cvijetić, I., Gašić, U., Čutović, N., Pešić, M., Milinčić, D., Carević, T., Marinković, A., Bugarski, B.,& Marković, T.. (2024). Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia. in Pharmaceuticals
Basel: MDPI., 17(4), 518.
https://doi.org/10.3390/ph17040518

Batinić P, Jovanović A, Stojković D, Zengin G, Cvijetić I, Gašić U, Čutović N, Pešić M, Milinčić D, Carević T, Marinković A, Bugarski B, Marković T. Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia. in Pharmaceuticals. 2024;17(4):518.
doi:10.3390/ph17040518 .

Batinić, Petar, Jovanović, Aleksandra, Stojković, Dejan, Zengin, Gokhan, Cvijetić, Ilija, Gašić, Uroš, Čutović, Natalija, Pešić, Mirjana, Milinčić, Danijel, Carević, Tamara, Marinković, Aleksandar, Bugarski, Branko, Marković, Tatjana, "Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia" in Pharmaceuticals, 17, no. 4 (2024):518,
https://doi.org/10.3390/ph17040518 . .

TY  - JOUR
AU  - Batinić, Petar
AU  - Čutović, Natalija
AU  - Jovanović, Aleksandra
AU  - Stojković, Dejan
AU  - Zengin, Gokhan
AU  - Carević, Tamara
AU  - Prijić, Željana
AU  - Marinković, Aleksandar
AU  - Marković, Tatjana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6680
AB  - This study was designed to investigate the impact of different extraction methods (ultrasound-assisted extraction, maceration, and microwave-assisted extraction) on the content of polyphenols and biological activities of leaf extract of Paeonia daurica subsp. daurica collected in Južni Kučaj (Serbia). The total polyphenol and flavonoid content were achieved by the UV-Vis spectroscopy. FTIR analysis was conducted to investigate structural properties of the extracts. In vitro methods (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) - ABTS and 2,2-diphenyl-1-picrylhydrazyl – DPPH assays) were carried out to measure the antioxidant activity of the extracts, while their antibacterial activity was tested against three Gram-positive and three Gram-negative bacterial strains. Analyzing the results of antioxidant activity, the highest anti-DPPH potential was achieved in the extracts of LPD obtained by maceration. The extraction method proven to produce the most effective antibacterial agents is maceration. Moreover, inhibitory activity of extracts against acetyl–and butyrylcholinesterase, α-amylase, α-glucosidase, and tyrosinase was evaluated in the enzyme inhibition assays. Overall, the results of this research indicated the possible use utilization of leaf extracts of Paeonia daurica subsp. daurica Andrews in food technology and pharmacy.
PB  - Belgrade: Institute for Medicinal Plant Research "Dr. Josif Pančić"
T2  - Lekovite sirovine
T1  - Antioxidant, antibacterial and enzyme inhibitory activity of the leaf extracts of Paeonia daurica Andrews wild growing in Serbia
IS  - 1
VL  - 43
DO  - 10.61652/leksir2343e159B
SP  - e159
ER  - 

@article{
author = "Batinić, Petar and Čutović, Natalija and Jovanović, Aleksandra and Stojković, Dejan and Zengin, Gokhan and Carević, Tamara and Prijić, Željana and Marinković, Aleksandar and Marković, Tatjana",
year = "2023",
abstract = "This study was designed to investigate the impact of different extraction methods (ultrasound-assisted extraction, maceration, and microwave-assisted extraction) on the content of polyphenols and biological activities of leaf extract of Paeonia daurica subsp. daurica collected in Južni Kučaj (Serbia). The total polyphenol and flavonoid content were achieved by the UV-Vis spectroscopy. FTIR analysis was conducted to investigate structural properties of the extracts. In vitro methods (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) - ABTS and 2,2-diphenyl-1-picrylhydrazyl – DPPH assays) were carried out to measure the antioxidant activity of the extracts, while their antibacterial activity was tested against three Gram-positive and three Gram-negative bacterial strains. Analyzing the results of antioxidant activity, the highest anti-DPPH potential was achieved in the extracts of LPD obtained by maceration. The extraction method proven to produce the most effective antibacterial agents is maceration. Moreover, inhibitory activity of extracts against acetyl–and butyrylcholinesterase, α-amylase, α-glucosidase, and tyrosinase was evaluated in the enzyme inhibition assays. Overall, the results of this research indicated the possible use utilization of leaf extracts of Paeonia daurica subsp. daurica Andrews in food technology and pharmacy.",
publisher = "Belgrade: Institute for Medicinal Plant Research "Dr. Josif Pančić"",
journal = "Lekovite sirovine",
title = "Antioxidant, antibacterial and enzyme inhibitory activity of the leaf extracts of Paeonia daurica Andrews wild growing in Serbia",
number = "1",
volume = "43",
doi = "10.61652/leksir2343e159B",
pages = "e159"
}

Batinić, P., Čutović, N., Jovanović, A., Stojković, D., Zengin, G., Carević, T., Prijić, Ž., Marinković, A.,& Marković, T.. (2023). Antioxidant, antibacterial and enzyme inhibitory activity of the leaf extracts of Paeonia daurica Andrews wild growing in Serbia. in Lekovite sirovine
Belgrade: Institute for Medicinal Plant Research "Dr. Josif Pančić"., 43(1), e159.
https://doi.org/10.61652/leksir2343e159B

Batinić P, Čutović N, Jovanović A, Stojković D, Zengin G, Carević T, Prijić Ž, Marinković A, Marković T. Antioxidant, antibacterial and enzyme inhibitory activity of the leaf extracts of Paeonia daurica Andrews wild growing in Serbia. in Lekovite sirovine. 2023;43(1):e159.
doi:10.61652/leksir2343e159B .

Batinić, Petar, Čutović, Natalija, Jovanović, Aleksandra, Stojković, Dejan, Zengin, Gokhan, Carević, Tamara, Prijić, Željana, Marinković, Aleksandar, Marković, Tatjana, "Antioxidant, antibacterial and enzyme inhibitory activity of the leaf extracts of Paeonia daurica Andrews wild growing in Serbia" in Lekovite sirovine, 43, no. 1 (2023):e159,
https://doi.org/10.61652/leksir2343e159B . .

TY  - JOUR
AU  - Alimpić-Aradski, Ana
AU  - Olađe Pavlović, Mariana
AU  - Janošević, Dušica
AU  - Todorović, Slađana
AU  - Gašić, Uroš
AU  - Mišić, Danijela
AU  - Pljevljakušić, Dejan
AU  - Šavikin, Katarina
AU  - Marin, Petar D.
AU  - Giweli, Abdulhmid
AU  - Duletić-Laušević, Sonja
PY  - 2023
UR  - http://www3.interscience.wiley.com/cgi-bin/jhome/5152
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6445
AB  - Introduction: The endemic species Nepeta cyrenaica Quézel & Zaffran, native to northeastern Libya, is valued as an important honey-bearing plant.

Objectives: This study was aimed to examine the micromorphology, phytochemistry, and bioactivity of in vitro-propagated N. cyrenaica for the first time.

Materials and methods: The leaf indumentum was examined using light and scanning electron microscopy and further characterised for histochemistry. The chemical composition of essential oil (EO) was performed using GC-MS analysis, while dichloromethane (DCM), methanol (ME), ethanol (ET), and aqueous (AQ) extracts were analysed using qualitative and quantitative LC/MS analyses. The antioxidant activities of EO and extracts were assessed using three parallel assays, while enzyme-inhibiting effects were evaluated against four enzymes.

Results: The leaves bear various types of glandular trichomes, with lipophilic secretion predominating. The main EO component of EO was 1,8-cineole. A considerable number of phenolics and iridoids were tentatively identified in the ME extract. Quantitative LC/MS analysis confirmed that ferulic acid, rosmarinic acid, and epigallocatechin gallate were present in the highest amount in the extracts, in which three iridoids were also quantified. Although the ME extract contained the highest amount of polyphenolics and iridoids, the DCM extract showed the best overall biological potential. Additionally, EO exerted the strongest acetylcholinesterase and tyrosinase inhibition.

Conclusion: This study demonstrated that the endemic N. cyrenaica can be efficiently grown under in vitro conditions, where it develops various glandular trichomes that are thought to secrete and/or accumulate bioactive compounds with valuable medicinal potential.
PB  - Hoboken: John Wiley and Sons
T2  - Phytochemical Analysis
T1  - Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae)
IS  - 6
VL  - 34
DO  - 10.1002/pca.3257
SP  - 661
EP  - 679
ER  - 

@article{
author = "Alimpić-Aradski, Ana and Olađe Pavlović, Mariana and Janošević, Dušica and Todorović, Slađana and Gašić, Uroš and Mišić, Danijela and Pljevljakušić, Dejan and Šavikin, Katarina and Marin, Petar D. and Giweli, Abdulhmid and Duletić-Laušević, Sonja",
year = "2023",
abstract = "Introduction: The endemic species Nepeta cyrenaica Quézel & Zaffran, native to northeastern Libya, is valued as an important honey-bearing plant.

Objectives: This study was aimed to examine the micromorphology, phytochemistry, and bioactivity of in vitro-propagated N. cyrenaica for the first time.

Materials and methods: The leaf indumentum was examined using light and scanning electron microscopy and further characterised for histochemistry. The chemical composition of essential oil (EO) was performed using GC-MS analysis, while dichloromethane (DCM), methanol (ME), ethanol (ET), and aqueous (AQ) extracts were analysed using qualitative and quantitative LC/MS analyses. The antioxidant activities of EO and extracts were assessed using three parallel assays, while enzyme-inhibiting effects were evaluated against four enzymes.

Results: The leaves bear various types of glandular trichomes, with lipophilic secretion predominating. The main EO component of EO was 1,8-cineole. A considerable number of phenolics and iridoids were tentatively identified in the ME extract. Quantitative LC/MS analysis confirmed that ferulic acid, rosmarinic acid, and epigallocatechin gallate were present in the highest amount in the extracts, in which three iridoids were also quantified. Although the ME extract contained the highest amount of polyphenolics and iridoids, the DCM extract showed the best overall biological potential. Additionally, EO exerted the strongest acetylcholinesterase and tyrosinase inhibition.

Conclusion: This study demonstrated that the endemic N. cyrenaica can be efficiently grown under in vitro conditions, where it develops various glandular trichomes that are thought to secrete and/or accumulate bioactive compounds with valuable medicinal potential.",
publisher = "Hoboken: John Wiley and Sons",
journal = "Phytochemical Analysis",
title = "Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae)",
number = "6",
volume = "34",
doi = "10.1002/pca.3257",
pages = "661-679"
}

Alimpić-Aradski, A., Olađe Pavlović, M., Janošević, D., Todorović, S., Gašić, U., Mišić, D., Pljevljakušić, D., Šavikin, K., Marin, P. D., Giweli, A.,& Duletić-Laušević, S.. (2023). Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae). in Phytochemical Analysis
Hoboken: John Wiley and Sons., 34(6), 661-679.
https://doi.org/10.1002/pca.3257

Alimpić-Aradski A, Olađe Pavlović M, Janošević D, Todorović S, Gašić U, Mišić D, Pljevljakušić D, Šavikin K, Marin PD, Giweli A, Duletić-Laušević S. Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae). in Phytochemical Analysis. 2023;34(6):661-679.
doi:10.1002/pca.3257 .

Alimpić-Aradski, Ana, Olađe Pavlović, Mariana, Janošević, Dušica, Todorović, Slađana, Gašić, Uroš, Mišić, Danijela, Pljevljakušić, Dejan, Šavikin, Katarina, Marin, Petar D., Giweli, Abdulhmid, Duletić-Laušević, Sonja, "Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae)" in Phytochemical Analysis, 34, no. 6 (2023):661-679,
https://doi.org/10.1002/pca.3257 . .

TY  - JOUR
AU  - Đorđević, Sofija
AU  - Nedić, Nebojša
AU  - Pavlović, Aleksandra
AU  - Milojković-Opsenica, Dušanka
AU  - Tešić, Živoslav
AU  - Gašić, Uroš
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S2210803322000495
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5012
AB  - Introduction The aim of this study was to find the best conditions for the extraction of rutin and quercetin from Sophora flower in acacia honey as an extractant, and to formulate a new product – honey with added value. Methods For determination of the optimal extraction conditions of the above mentioned compounds, 10% m/m of the dried Sophora flower was added to honey and the concentration of rutin and quercetin was monitored, at 4 °C and room temperature at 1, 15, 40 and 90 day intervals. The analyses of rutin and quercetin content in honey were performed by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. The content of rutin and quercetin in the flower was also determined to check the quality of the herbal drug used, as well as to evaluate the effectiveness of the extraction. Results The extraction of rutin and quercetin, in a honey sample using 10% of the Sophora flower, stored at room temperature, for 40 days, was the most intense one (150.24 mg/kg of rutin and 1338.93 mg/kg of quercetin). Sophora flower corresponds to the pharmacopoeia requirements for the quality of an herbal drug. The concentrations of rutin and quercetin in acacia honey were 0.08 and 0.14 mg/kg, respectively. The concentration of rutin in honey from the melliferous pasture of the Sophora flower was 0.40 mg/kg, while quercetin was below the detection limit (0.1 mg/kg). Conclusions Honey, enriched with rutin and quercetin from Sophora flower, had sensory acceptability and pleasant taste and aroma.
T2  - Journal of Herbal Medicine
T1  - Honey with added value – enriched with rutin and quercetin from Sophora flower
VL  - 34
DO  - 10.1016/j.hermed.2022.100580
SP  - 100580
ER  - 

@article{
author = "Đorđević, Sofija and Nedić, Nebojša and Pavlović, Aleksandra and Milojković-Opsenica, Dušanka and Tešić, Živoslav and Gašić, Uroš",
year = "2022",
abstract = "Introduction The aim of this study was to find the best conditions for the extraction of rutin and quercetin from Sophora flower in acacia honey as an extractant, and to formulate a new product – honey with added value. Methods For determination of the optimal extraction conditions of the above mentioned compounds, 10% m/m of the dried Sophora flower was added to honey and the concentration of rutin and quercetin was monitored, at 4 °C and room temperature at 1, 15, 40 and 90 day intervals. The analyses of rutin and quercetin content in honey were performed by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. The content of rutin and quercetin in the flower was also determined to check the quality of the herbal drug used, as well as to evaluate the effectiveness of the extraction. Results The extraction of rutin and quercetin, in a honey sample using 10% of the Sophora flower, stored at room temperature, for 40 days, was the most intense one (150.24 mg/kg of rutin and 1338.93 mg/kg of quercetin). Sophora flower corresponds to the pharmacopoeia requirements for the quality of an herbal drug. The concentrations of rutin and quercetin in acacia honey were 0.08 and 0.14 mg/kg, respectively. The concentration of rutin in honey from the melliferous pasture of the Sophora flower was 0.40 mg/kg, while quercetin was below the detection limit (0.1 mg/kg). Conclusions Honey, enriched with rutin and quercetin from Sophora flower, had sensory acceptability and pleasant taste and aroma.",
journal = "Journal of Herbal Medicine",
title = "Honey with added value – enriched with rutin and quercetin from Sophora flower",
volume = "34",
doi = "10.1016/j.hermed.2022.100580",
pages = "100580"
}

Đorđević, S., Nedić, N., Pavlović, A., Milojković-Opsenica, D., Tešić, Ž.,& Gašić, U.. (2022). Honey with added value – enriched with rutin and quercetin from Sophora flower. in Journal of Herbal Medicine, 34, 100580.
https://doi.org/10.1016/j.hermed.2022.100580

Đorđević S, Nedić N, Pavlović A, Milojković-Opsenica D, Tešić Ž, Gašić U. Honey with added value – enriched with rutin and quercetin from Sophora flower. in Journal of Herbal Medicine. 2022;34:100580.
doi:10.1016/j.hermed.2022.100580 .

Đorđević, Sofija, Nedić, Nebojša, Pavlović, Aleksandra, Milojković-Opsenica, Dušanka, Tešić, Živoslav, Gašić, Uroš, "Honey with added value – enriched with rutin and quercetin from Sophora flower" in Journal of Herbal Medicine, 34 (2022):100580,
https://doi.org/10.1016/j.hermed.2022.100580 . .

TY  - JOUR
AU  - Marinković, Jelena
AU  - Marković, Tatjana
AU  - Nikolić, Biljana
AU  - Ćirić, Ana
AU  - Mitić-Ćulafić, Dragana
AU  - Dukanović, Stefana
AU  - Krstić, Aleksandar
AU  - Pavlica, Dušan
AU  - Vlajić, Tamara
AU  - Marković, Dejan
PY  - 2022
UR  - https://www.tandfonline.com/doi/full/10.1080/0972060X.2022.2040386
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4944
AB  - The aim of the study was to investigate the potency of Cinnamomum camphora cineoliferum and Melaleuca ericifolia essential oils (EOs) against facultative and obligate anaerobes isolated from teeth’ infected root canals. Biocompatibility of the EOs was also in the study focus. Chemical composition of the EOs was determined by GC/MS analysis. Their antibacterial potential and cytotoxicity were evaluated by microdilution and MTT assays, respectively. Statistical analysis was performed by Student’s t-test and one way ANOVA. Two the most abundant constituents of C. camphora cineoliferum EO were 1,8-cineole and sabinene, while in M. ericifolia EO the dominant were linalool and 1,8-cineole. MICs achieved against all tested strains were in the ranges 0.95±0.50 - 22.50±0.00 mg/mL and 0.65±0.00 - 23.35±7.80 mg/mL for the C. camphora cioneoliferum and M. ericifolia EOs, respectively. Statistical analysis showed that response to both oils has not differed between facultative and obligate anaerobes. Sensitivity pattern of the tested strains was different for the EOs and the positive control triple antibiotic paste (TAP): while E. faecalis and certain strains of S. salivarius were among the most sensitive to the oils, they were the least susceptible to the conventional antimicrobial TAP. Although the observed cytotoxicity of the EOs after 30 min of cell exposition was high (50.78-66.35% and up to 70% for C. camphora cioneoliferum and M. ericifolia EOs, respectively) it was lower than that of positive control (1.5% NaOCl, p<0.05). To conclude, both EOs showed promising antibacterial potential and achieved at least the same or even better biocompatibility than the conventional intracanal antimicrobials. Obtained results strongly encourage future research with an aim to formulate C. camphora cioneoliferum and M. ericifolia EO- based irrigants and medicaments, that could be used in endodontic practice.
PB  - Abingdon: Taylor & Francis Ltd.
T2  - Journal of Essential Oil-Bearing Plants
T1  - Biocompatibility and Antibacterial Potential of the Cinnamomum camphora cineoliferum (L.) J. Presl. and Melaleuca ericifolia Sm. Essential Oils Against Facultative and Obligate Endodontic Anaerobes
IS  - 1
VL  - 25
DO  - 10.1080/0972060X.2022.2040386
SP  - 111
EP  - 125
ER  - 

@article{
author = "Marinković, Jelena and Marković, Tatjana and Nikolić, Biljana and Ćirić, Ana and Mitić-Ćulafić, Dragana and Dukanović, Stefana and Krstić, Aleksandar and Pavlica, Dušan and Vlajić, Tamara and Marković, Dejan",
year = "2022",
abstract = "The aim of the study was to investigate the potency of Cinnamomum camphora cineoliferum and Melaleuca ericifolia essential oils (EOs) against facultative and obligate anaerobes isolated from teeth’ infected root canals. Biocompatibility of the EOs was also in the study focus. Chemical composition of the EOs was determined by GC/MS analysis. Their antibacterial potential and cytotoxicity were evaluated by microdilution and MTT assays, respectively. Statistical analysis was performed by Student’s t-test and one way ANOVA. Two the most abundant constituents of C. camphora cineoliferum EO were 1,8-cineole and sabinene, while in M. ericifolia EO the dominant were linalool and 1,8-cineole. MICs achieved against all tested strains were in the ranges 0.95±0.50 - 22.50±0.00 mg/mL and 0.65±0.00 - 23.35±7.80 mg/mL for the C. camphora cioneoliferum and M. ericifolia EOs, respectively. Statistical analysis showed that response to both oils has not differed between facultative and obligate anaerobes. Sensitivity pattern of the tested strains was different for the EOs and the positive control triple antibiotic paste (TAP): while E. faecalis and certain strains of S. salivarius were among the most sensitive to the oils, they were the least susceptible to the conventional antimicrobial TAP. Although the observed cytotoxicity of the EOs after 30 min of cell exposition was high (50.78-66.35% and up to 70% for C. camphora cioneoliferum and M. ericifolia EOs, respectively) it was lower than that of positive control (1.5% NaOCl, p<0.05). To conclude, both EOs showed promising antibacterial potential and achieved at least the same or even better biocompatibility than the conventional intracanal antimicrobials. Obtained results strongly encourage future research with an aim to formulate C. camphora cioneoliferum and M. ericifolia EO- based irrigants and medicaments, that could be used in endodontic practice.",
publisher = "Abingdon: Taylor & Francis Ltd.",
journal = "Journal of Essential Oil-Bearing Plants",
title = "Biocompatibility and Antibacterial Potential of the Cinnamomum camphora cineoliferum (L.) J. Presl. and Melaleuca ericifolia Sm. Essential Oils Against Facultative and Obligate Endodontic Anaerobes",
number = "1",
volume = "25",
doi = "10.1080/0972060X.2022.2040386",
pages = "111-125"
}

Marinković, J., Marković, T., Nikolić, B., Ćirić, A., Mitić-Ćulafić, D., Dukanović, S., Krstić, A., Pavlica, D., Vlajić, T.,& Marković, D.. (2022). Biocompatibility and Antibacterial Potential of the Cinnamomum camphora cineoliferum (L.) J. Presl. and Melaleuca ericifolia Sm. Essential Oils Against Facultative and Obligate Endodontic Anaerobes. in Journal of Essential Oil-Bearing Plants
Abingdon: Taylor & Francis Ltd.., 25(1), 111-125.
https://doi.org/10.1080/0972060X.2022.2040386

Marinković J, Marković T, Nikolić B, Ćirić A, Mitić-Ćulafić D, Dukanović S, Krstić A, Pavlica D, Vlajić T, Marković D. Biocompatibility and Antibacterial Potential of the Cinnamomum camphora cineoliferum (L.) J. Presl. and Melaleuca ericifolia Sm. Essential Oils Against Facultative and Obligate Endodontic Anaerobes. in Journal of Essential Oil-Bearing Plants. 2022;25(1):111-125.
doi:10.1080/0972060X.2022.2040386 .

Marinković, Jelena, Marković, Tatjana, Nikolić, Biljana, Ćirić, Ana, Mitić-Ćulafić, Dragana, Dukanović, Stefana, Krstić, Aleksandar, Pavlica, Dušan, Vlajić, Tamara, Marković, Dejan, "Biocompatibility and Antibacterial Potential of the Cinnamomum camphora cineoliferum (L.) J. Presl. and Melaleuca ericifolia Sm. Essential Oils Against Facultative and Obligate Endodontic Anaerobes" in Journal of Essential Oil-Bearing Plants, 25, no. 1 (2022):111-125,
https://doi.org/10.1080/0972060X.2022.2040386 . .

TY  - JOUR
AU  - Đorđevski, Nikoleta
AU  - Stojković, Dejan
AU  - Živković, Jelena
AU  - Pljevljakušić, Dejan
AU  - Ristanović, Elizabeta
AU  - Nikolić, Biljana
AU  - Ćirić, Ana
PY  - 2022
UR  - https://onlinelibrary.wiley.com/doi/10.1002/fsn3.2777
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4864
AB  - This study was designed to explore functional food properties of edible seed oil obtained from Tamjanika seeds—autochthonous grape variety of Balkan Peninsula. In order to accomplish our goals, seed oil was isolated by Soxhlet apparatus and chemically characterized regarding fatty acids, carotenoids, tocopherols, and tocotrienols. Antimicrobial activity of the isolated oil was tested by microdilution method. For that purposes, six bacterial species were used, belonging to human infectious agents and food contaminants. Furthermore, the activity of the oil was investigated against clinical isolates of dermatomycetes. Our study has shown that oil of Vitis vinifera L. Tamjanika variety was an abundant source of polyunsaturated fatty acids (81.43%) with predominant linoleic acid. HPLC analysis revealed the presence of carotenoid lutein (0.15 mg/100 g). The seed oil was rich in tocotrienols (85.04 mg/100 g) predominating over tocopherols (8.37 mg/100 g). The oil possessed microbicidal activity against all the tested microbes. Bacteria were more sensitive to the effect of the oil (minimum inhibitory concentration [MIC] 7.7–15.4) when compared with oil effect on tested dermatomycetes (MIC 20–40). Our investigation has shown for the first time that grape oil could be active against wide spectrum of bacteria and clinically isolated dermatomycetes. The significance of this study lies in the fact that it pointed out the functional food properties of grape seed oil that was fully chemically characterized.
PB  - John Wiley and Sons Inc
T2  - Food Science & Nutrition
T1  - Tamjanika, a Balkan native variety of Vitis vinifera L.: Chemical characterization, antibacterial, and anti‐dermatomycosis potential of seed oil
IS  - 4
VL  - 10
DO  - 10.1002/fsn3.2777
SP  - 1312
EP  - 1319
ER  - 

@article{
author = "Đorđevski, Nikoleta and Stojković, Dejan and Živković, Jelena and Pljevljakušić, Dejan and Ristanović, Elizabeta and Nikolić, Biljana and Ćirić, Ana",
year = "2022",
abstract = "This study was designed to explore functional food properties of edible seed oil obtained from Tamjanika seeds—autochthonous grape variety of Balkan Peninsula. In order to accomplish our goals, seed oil was isolated by Soxhlet apparatus and chemically characterized regarding fatty acids, carotenoids, tocopherols, and tocotrienols. Antimicrobial activity of the isolated oil was tested by microdilution method. For that purposes, six bacterial species were used, belonging to human infectious agents and food contaminants. Furthermore, the activity of the oil was investigated against clinical isolates of dermatomycetes. Our study has shown that oil of Vitis vinifera L. Tamjanika variety was an abundant source of polyunsaturated fatty acids (81.43%) with predominant linoleic acid. HPLC analysis revealed the presence of carotenoid lutein (0.15 mg/100 g). The seed oil was rich in tocotrienols (85.04 mg/100 g) predominating over tocopherols (8.37 mg/100 g). The oil possessed microbicidal activity against all the tested microbes. Bacteria were more sensitive to the effect of the oil (minimum inhibitory concentration [MIC] 7.7–15.4) when compared with oil effect on tested dermatomycetes (MIC 20–40). Our investigation has shown for the first time that grape oil could be active against wide spectrum of bacteria and clinically isolated dermatomycetes. The significance of this study lies in the fact that it pointed out the functional food properties of grape seed oil that was fully chemically characterized.",
publisher = "John Wiley and Sons Inc",
journal = "Food Science & Nutrition",
title = "Tamjanika, a Balkan native variety of Vitis vinifera L.: Chemical characterization, antibacterial, and anti‐dermatomycosis potential of seed oil",
number = "4",
volume = "10",
doi = "10.1002/fsn3.2777",
pages = "1312-1319"
}

Đorđevski, N., Stojković, D., Živković, J., Pljevljakušić, D., Ristanović, E., Nikolić, B.,& Ćirić, A.. (2022). Tamjanika, a Balkan native variety of Vitis vinifera L.: Chemical characterization, antibacterial, and anti‐dermatomycosis potential of seed oil. in Food Science & Nutrition
John Wiley and Sons Inc., 10(4), 1312-1319.
https://doi.org/10.1002/fsn3.2777

Đorđevski N, Stojković D, Živković J, Pljevljakušić D, Ristanović E, Nikolić B, Ćirić A. Tamjanika, a Balkan native variety of Vitis vinifera L.: Chemical characterization, antibacterial, and anti‐dermatomycosis potential of seed oil. in Food Science & Nutrition. 2022;10(4):1312-1319.
doi:10.1002/fsn3.2777 .

Đorđevski, Nikoleta, Stojković, Dejan, Živković, Jelena, Pljevljakušić, Dejan, Ristanović, Elizabeta, Nikolić, Biljana, Ćirić, Ana, "Tamjanika, a Balkan native variety of Vitis vinifera L.: Chemical characterization, antibacterial, and anti‐dermatomycosis potential of seed oil" in Food Science & Nutrition, 10, no. 4 (2022):1312-1319,
https://doi.org/10.1002/fsn3.2777 . .

TY  - JOUR
AU  - Veličković, Ivona
AU  - Živković, Jelena
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Marin, Petar D.
AU  - Grujić, Slavica
PY  - 2021
UR  - https://journals.sagepub.com/doi/10.1177/1934578X211009692
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4226
AB  - The presented study was aimed at exploring the bioactive properties (antioxidant, antimicrobial and food preserving) and chemical composition of R. discolor fruits collected from 2 geographically distinct wild-growing populations and extracted by using different solvents (methanol, ethanol, acetone and water). The total phenol (TPC), flavonoid (TFC) and anthocyanin (TAC) content varied from 52.7 to 186.8 mg GAE/g, 3.5 to 7.0 mg QE/g and 11.1 to 28.2 mg/g dry weight (dw), respectively. HPLC-DAD analysis identified cyanidin-3-glucoside (Cy-3-Glu) as the dominant anthocyanin that ranged in concentration from 18.4 to 32.2 mg/g dw. The acetone extract revealed the highest antioxidant activity through the ferric reducing antioxidant power (FRAP), total reducing power (TRC) and ß-carotene bleaching in vitro methods. The examined extracts showed antimicrobial activity, being more effective against Gram-positive (G+) than Gram-negative (G-) bacteria. Furthermore, Candida glabrata was the most susceptible among the pathogenic yeasts. The ethanol fruit extract was the most active against Listeria monocytogenes and thus chosen for incorporation in yoghurt and further analyses regarding its food preserving properties, which confirmed its efficacy towards this food-borne pathogen. Additionally, panelists gave high marks to the novel product regarding color, taste, texture flavor and overall acceptance. The obtained results indicated that R. discolor fruit extracts exhibits notable bioactivity and might therefore be considered as a good platform for the development of new preparations to be used in the food industry.
PB  - SAGE Publications Inc.
T2  - Natural Product Communications
T1  - Evaluation of Antioxidant, Antimicrobial and Potential Food Preserving Properties of Rubus Discolor (Rosaceae) Fruit Extracts
IS  - 4
VL  - 16
DO  - 10.1177/1934578X211009692
SP  - 1
EP  - 9
ER  - 

@article{
author = "Veličković, Ivona and Živković, Jelena and Stojković, Dejan and Soković, Marina and Marin, Petar D. and Grujić, Slavica",
year = "2021",
abstract = "The presented study was aimed at exploring the bioactive properties (antioxidant, antimicrobial and food preserving) and chemical composition of R. discolor fruits collected from 2 geographically distinct wild-growing populations and extracted by using different solvents (methanol, ethanol, acetone and water). The total phenol (TPC), flavonoid (TFC) and anthocyanin (TAC) content varied from 52.7 to 186.8 mg GAE/g, 3.5 to 7.0 mg QE/g and 11.1 to 28.2 mg/g dry weight (dw), respectively. HPLC-DAD analysis identified cyanidin-3-glucoside (Cy-3-Glu) as the dominant anthocyanin that ranged in concentration from 18.4 to 32.2 mg/g dw. The acetone extract revealed the highest antioxidant activity through the ferric reducing antioxidant power (FRAP), total reducing power (TRC) and ß-carotene bleaching in vitro methods. The examined extracts showed antimicrobial activity, being more effective against Gram-positive (G+) than Gram-negative (G-) bacteria. Furthermore, Candida glabrata was the most susceptible among the pathogenic yeasts. The ethanol fruit extract was the most active against Listeria monocytogenes and thus chosen for incorporation in yoghurt and further analyses regarding its food preserving properties, which confirmed its efficacy towards this food-borne pathogen. Additionally, panelists gave high marks to the novel product regarding color, taste, texture flavor and overall acceptance. The obtained results indicated that R. discolor fruit extracts exhibits notable bioactivity and might therefore be considered as a good platform for the development of new preparations to be used in the food industry.",
publisher = "SAGE Publications Inc.",
journal = "Natural Product Communications",
title = "Evaluation of Antioxidant, Antimicrobial and Potential Food Preserving Properties of Rubus Discolor (Rosaceae) Fruit Extracts",
number = "4",
volume = "16",
doi = "10.1177/1934578X211009692",
pages = "1-9"
}

Veličković, I., Živković, J., Stojković, D., Soković, M., Marin, P. D.,& Grujić, S.. (2021). Evaluation of Antioxidant, Antimicrobial and Potential Food Preserving Properties of Rubus Discolor (Rosaceae) Fruit Extracts. in Natural Product Communications
SAGE Publications Inc.., 16(4), 1-9.
https://doi.org/10.1177/1934578X211009692

Veličković I, Živković J, Stojković D, Soković M, Marin PD, Grujić S. Evaluation of Antioxidant, Antimicrobial and Potential Food Preserving Properties of Rubus Discolor (Rosaceae) Fruit Extracts. in Natural Product Communications. 2021;16(4):1-9.
doi:10.1177/1934578X211009692 .

Veličković, Ivona, Živković, Jelena, Stojković, Dejan, Soković, Marina, Marin, Petar D., Grujić, Slavica, "Evaluation of Antioxidant, Antimicrobial and Potential Food Preserving Properties of Rubus Discolor (Rosaceae) Fruit Extracts" in Natural Product Communications, 16, no. 4 (2021):1-9,
https://doi.org/10.1177/1934578X211009692 . .

TY  - JOUR
AU  - Oalđe, Mariana
AU  - Kolarević, Stoimir
AU  - Živković, Jelena
AU  - Alimpić Aradski, Ana
AU  - Jovanović Marić, Jovana
AU  - Kračun-Kolarević, Margareta
AU  - Đorđević, Jelena
AU  - Marin, Petar D.
AU  - Šavikin, Katarina
AU  - Vuković-Gačić, Branka
AU  - Duletić-Laušević, Sonja
PY  - 2021
UR  - https://pubs.rsc.org/en/content/articlehtml/2021/fo/d1fo00447f
UR  - http://xlink.rsc.org/?DOI=D1FO00447F
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4217
AB  - This research was aimed to assess the potential of Glechoma hederacea, Hyssopus officinalis, Lavandula angustifolia, Leonurus cardiaca, Marrubium vulgare and Sideritis scardica (Lamiaceae) methanolic, ethanolic and aqueous extracts against the damaging effects of oxidative stress using different experimental models. The chemical characterization was done spectrophotometrically by quantifying total phenolics, phenolic acids, flavonoids and flavonols in the extracts, as well as by employing HPLC-DAD technique. Moreover, DPPH assay was used to assess the extracts' radical scavenging potential. Genoprotective properties of the extracts were evaluated using plasmid pUC19 Escherichia coli XL1-Blue, whereas their antigenotoxic potential was determined using Salmonella typhimurium TA1535/pSK1002 and normal human lung fibroblasts. All of the extracts showed antioxidant activity in DPPH assay. Furthermore, the results have shown that aqueous extracts provided the best protection for plasmid DNA, while alcoholic extracts most effectively contributed to the preservation of prokaryotic DNA. Additionally, each of the tested samples significantly protected the eukaryotic cells against genomic damages. Finally, despite not showing exceptional results in DPPH assay, S. scardica extracts are regarded as the most favorable in maintaining the integrity of DNA, which might be due to high quantities of phenolics such as quercetin (up to 17.95 mg g-1), naringin (up to 5.07 mg g-1) and luteolin-7-O-glucoside (up to 3.54 mg g-1). Overall, this comprehensive concept highlights the ability of these Lamiaceae species to safeguard the DNA from reactive oxygen species, to curtail the inflicted damage and also improve the efficiency of the DNA repair mechanisms, while emphasizing the importance of polyphenols as their active principles.
PB  - Royal Society of Chemistry (RSC)
T2  - Food & Function
T1  - A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro
IS  - 7
VL  - 12
DO  - 10.1039/d1fo00447f
SP  - 3233
EP  - 3245
ER  - 

@article{
author = "Oalđe, Mariana and Kolarević, Stoimir and Živković, Jelena and Alimpić Aradski, Ana and Jovanović Marić, Jovana and Kračun-Kolarević, Margareta and Đorđević, Jelena and Marin, Petar D. and Šavikin, Katarina and Vuković-Gačić, Branka and Duletić-Laušević, Sonja",
year = "2021",
abstract = "This research was aimed to assess the potential of Glechoma hederacea, Hyssopus officinalis, Lavandula angustifolia, Leonurus cardiaca, Marrubium vulgare and Sideritis scardica (Lamiaceae) methanolic, ethanolic and aqueous extracts against the damaging effects of oxidative stress using different experimental models. The chemical characterization was done spectrophotometrically by quantifying total phenolics, phenolic acids, flavonoids and flavonols in the extracts, as well as by employing HPLC-DAD technique. Moreover, DPPH assay was used to assess the extracts' radical scavenging potential. Genoprotective properties of the extracts were evaluated using plasmid pUC19 Escherichia coli XL1-Blue, whereas their antigenotoxic potential was determined using Salmonella typhimurium TA1535/pSK1002 and normal human lung fibroblasts. All of the extracts showed antioxidant activity in DPPH assay. Furthermore, the results have shown that aqueous extracts provided the best protection for plasmid DNA, while alcoholic extracts most effectively contributed to the preservation of prokaryotic DNA. Additionally, each of the tested samples significantly protected the eukaryotic cells against genomic damages. Finally, despite not showing exceptional results in DPPH assay, S. scardica extracts are regarded as the most favorable in maintaining the integrity of DNA, which might be due to high quantities of phenolics such as quercetin (up to 17.95 mg g-1), naringin (up to 5.07 mg g-1) and luteolin-7-O-glucoside (up to 3.54 mg g-1). Overall, this comprehensive concept highlights the ability of these Lamiaceae species to safeguard the DNA from reactive oxygen species, to curtail the inflicted damage and also improve the efficiency of the DNA repair mechanisms, while emphasizing the importance of polyphenols as their active principles.",
publisher = "Royal Society of Chemistry (RSC)",
journal = "Food & Function",
title = "A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro",
number = "7",
volume = "12",
doi = "10.1039/d1fo00447f",
pages = "3233-3245"
}

Oalđe, M., Kolarević, S., Živković, J., Alimpić Aradski, A., Jovanović Marić, J., Kračun-Kolarević, M., Đorđević, J., Marin, P. D., Šavikin, K., Vuković-Gačić, B.,& Duletić-Laušević, S.. (2021). A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro. in Food & Function
Royal Society of Chemistry (RSC)., 12(7), 3233-3245.
https://doi.org/10.1039/d1fo00447f

Oalđe M, Kolarević S, Živković J, Alimpić Aradski A, Jovanović Marić J, Kračun-Kolarević M, Đorđević J, Marin PD, Šavikin K, Vuković-Gačić B, Duletić-Laušević S. A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro. in Food & Function. 2021;12(7):3233-3245.
doi:10.1039/d1fo00447f .

Oalđe, Mariana, Kolarević, Stoimir, Živković, Jelena, Alimpić Aradski, Ana, Jovanović Marić, Jovana, Kračun-Kolarević, Margareta, Đorđević, Jelena, Marin, Petar D., Šavikin, Katarina, Vuković-Gačić, Branka, Duletić-Laušević, Sonja, "A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro" in Food & Function, 12, no. 7 (2021):3233-3245,
https://doi.org/10.1039/d1fo00447f . .

TY  - JOUR
AU  - Živković, Jelena
AU  - Ivanov, Marija
AU  - Stojković, Dejan
AU  - Glamočlija, Jasmina
PY  - 2021
UR  - https://doi.org/10.3390/jof7050349
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4243
AB  - This study aims to fill the gaps in ethnomycological knowledge in Serbia by identifying various fungal species that have been used due to their medicinal or nutritional properties. Eth-nomycological information was gathered using semi-structured interviews with participants from different mycological associations in Serbia. A total of 62 participants were involved in this study. Eighty-five species belonging to 28 families were identified. All of the reported fungal species were pointed out as edible, and only 15 of them were declared as medicinal. The family Boletaceae was represented by the highest number of species, followed by Russulaceae, Agaricaceae and Polypo-raceae. We also performed detailed analysis of the literature in order to provide scientific evidence for the recorded medicinal use of fungi in Serbia. The male participants reported a higher level of ethnomycological knowledge compared to women, whereas the highest number of used fungi species was mentioned by participants within the age group of 61–80 years. In addition to pre-serving ethnomycological knowledge in Serbia, this study can present a good starting point for further pharmacological investigations of fungi.
PB  - MDPI AG
T2  - Journal of Fungi
T1  - Ethnomycological investigation in serbia: Astonishing realm of mycomedicines and mycofood
IS  - 5
VL  - 7
DO  - 10.3390/jof7050349
SP  - 349
ER  - 

@article{
author = "Živković, Jelena and Ivanov, Marija and Stojković, Dejan and Glamočlija, Jasmina",
year = "2021",
abstract = "This study aims to fill the gaps in ethnomycological knowledge in Serbia by identifying various fungal species that have been used due to their medicinal or nutritional properties. Eth-nomycological information was gathered using semi-structured interviews with participants from different mycological associations in Serbia. A total of 62 participants were involved in this study. Eighty-five species belonging to 28 families were identified. All of the reported fungal species were pointed out as edible, and only 15 of them were declared as medicinal. The family Boletaceae was represented by the highest number of species, followed by Russulaceae, Agaricaceae and Polypo-raceae. We also performed detailed analysis of the literature in order to provide scientific evidence for the recorded medicinal use of fungi in Serbia. The male participants reported a higher level of ethnomycological knowledge compared to women, whereas the highest number of used fungi species was mentioned by participants within the age group of 61–80 years. In addition to pre-serving ethnomycological knowledge in Serbia, this study can present a good starting point for further pharmacological investigations of fungi.",
publisher = "MDPI AG",
journal = "Journal of Fungi",
title = "Ethnomycological investigation in serbia: Astonishing realm of mycomedicines and mycofood",
number = "5",
volume = "7",
doi = "10.3390/jof7050349",
pages = "349"
}

Živković, J., Ivanov, M., Stojković, D.,& Glamočlija, J.. (2021). Ethnomycological investigation in serbia: Astonishing realm of mycomedicines and mycofood. in Journal of Fungi
MDPI AG., 7(5), 349.
https://doi.org/10.3390/jof7050349

Živković J, Ivanov M, Stojković D, Glamočlija J. Ethnomycological investigation in serbia: Astonishing realm of mycomedicines and mycofood. in Journal of Fungi. 2021;7(5):349.
doi:10.3390/jof7050349 .

Živković, Jelena, Ivanov, Marija, Stojković, Dejan, Glamočlija, Jasmina, "Ethnomycological investigation in serbia: Astonishing realm of mycomedicines and mycofood" in Journal of Fungi, 7, no. 5 (2021):349,
https://doi.org/10.3390/jof7050349 . .

TY  - JOUR
AU  - Marinković, Jelena
AU  - Nikolić, Biljana
AU  - Marković, Tatjana
AU  - Radulović, Milena
AU  - Ilić, Jugoslav
AU  - Bošković, Marko
AU  - Ćirić, Ana
AU  - Marković, Dejan
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4360
AB  - The objective was to formulate and characterize the nanoemulsion based on Cymbopogon citratus
oil, intended for use in teeth infected root canal therapy. The investigation of the antioxidant and an tibiofilm potential toward Enterococcus faecalis was aimed as well. Materials & methods: Characterization
of oil (by GC/MS analysis) and nanoemulsion (by dynamic light scattering instrument), and determination
of antibacterial (by microdilution assay), antibiofilm (by crystal violet assay) and antioxidant properties (by
2,2-diphenyl-1-picryl-hydrazyl-hydrate and thiobarbituric acid assay methods) were provided. Antibiofilm
efficacy of irrigation procedure including nanoemulsion was screened on extracted teeth (by CFU count ing assay). Results: Notable antibacterial and antibiofilm activity, both against forming and preformed
biofilms of oil, was observed. Irrigation involved nanoemulsion showed remarkable antibiofilm potential.
Both substances induced some antioxidant activity. Conclusion: Results encourage further research with
the aim of application of the nanoemulsion in dental practice.
PB  - London: Future Medicine Ltd.
T2  - Future Microbiology
T1  - Cymbopogon citratus essential oil: an active principle of nanoemulsion against Enterococcus faecalis root canal biofilm
IS  - 12
VL  - 16
DO  - 10.2217/fmb-2021-0081
SP  - 907
EP  - 918
ER  - 

@article{
author = "Marinković, Jelena and Nikolić, Biljana and Marković, Tatjana and Radulović, Milena and Ilić, Jugoslav and Bošković, Marko and Ćirić, Ana and Marković, Dejan",
year = "2021",
abstract = "The objective was to formulate and characterize the nanoemulsion based on Cymbopogon citratus
oil, intended for use in teeth infected root canal therapy. The investigation of the antioxidant and an tibiofilm potential toward Enterococcus faecalis was aimed as well. Materials & methods: Characterization
of oil (by GC/MS analysis) and nanoemulsion (by dynamic light scattering instrument), and determination
of antibacterial (by microdilution assay), antibiofilm (by crystal violet assay) and antioxidant properties (by
2,2-diphenyl-1-picryl-hydrazyl-hydrate and thiobarbituric acid assay methods) were provided. Antibiofilm
efficacy of irrigation procedure including nanoemulsion was screened on extracted teeth (by CFU count ing assay). Results: Notable antibacterial and antibiofilm activity, both against forming and preformed
biofilms of oil, was observed. Irrigation involved nanoemulsion showed remarkable antibiofilm potential.
Both substances induced some antioxidant activity. Conclusion: Results encourage further research with
the aim of application of the nanoemulsion in dental practice.",
publisher = "London: Future Medicine Ltd.",
journal = "Future Microbiology",
title = "Cymbopogon citratus essential oil: an active principle of nanoemulsion against Enterococcus faecalis root canal biofilm",
number = "12",
volume = "16",
doi = "10.2217/fmb-2021-0081",
pages = "907-918"
}

Marinković, J., Nikolić, B., Marković, T., Radulović, M., Ilić, J., Bošković, M., Ćirić, A.,& Marković, D.. (2021). Cymbopogon citratus essential oil: an active principle of nanoemulsion against Enterococcus faecalis root canal biofilm. in Future Microbiology
London: Future Medicine Ltd.., 16(12), 907-918.
https://doi.org/10.2217/fmb-2021-0081

Marinković J, Nikolić B, Marković T, Radulović M, Ilić J, Bošković M, Ćirić A, Marković D. Cymbopogon citratus essential oil: an active principle of nanoemulsion against Enterococcus faecalis root canal biofilm. in Future Microbiology. 2021;16(12):907-918.
doi:10.2217/fmb-2021-0081 .

Marinković, Jelena, Nikolić, Biljana, Marković, Tatjana, Radulović, Milena, Ilić, Jugoslav, Bošković, Marko, Ćirić, Ana, Marković, Dejan, "Cymbopogon citratus essential oil: an active principle of nanoemulsion against Enterococcus faecalis root canal biofilm" in Future Microbiology, 16, no. 12 (2021):907-918,
https://doi.org/10.2217/fmb-2021-0081 . .

TY  - JOUR
AU  - Savić, Aleksandra
AU  - Alimpić Aradski, Ana
AU  - Živković, Jelena
AU  - Šavikin, Katarina
AU  - Jarić, Snežana
AU  - Marin, Petar
AU  - Duletić-Laušević, Sonja
PY  - 2021
UR  - http://journal.pan.olsztyn.pl/Phenolic-Composition-and-Antioxidant-and-Antineurodegenerative-Potential-of-Methanolic,138320,0,2.html
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4416
AB  - Pear (Pyrus communis L.) is consumed as fresh fruit, in numerous food products, and also used as a traditional remedy in various countries, including Serbia. In search of bioactive compounds, six traditional pear varieties (‘Vidovača’, ‘Lubeničarka’, ‘Karamanka’, ‘Jeribasma’, ‘Lončara’, ‘Takiša’) and wild pear from Serbia were investigated and compared with a commercial variety (‘Williams Bartlett’). The aim of this study was to determine the total phenolic and flavonoid contents, phenolic composition, antioxidant capacity, and antineurodegenerative activities of methanolic extracts of peel, flesh, and mixed peel and flesh of pear fruits. Phenolic composition of extracts was determined with HPLC-DAD, while the antioxidant activity of extracts was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), and ferric-reducing antioxidant power (FRAP) assays. Bearing in mind that oxidative stress is closely linked to neurodegeneration, the antineurodegenerative potential of the extracts was assessed by the inhibition of acetycholineserase (AChE) and tyrosinase (TYR) activities. The extracts of traditional varieties, particularly peel extracts, had a high content of phenolics, as well as significant antioxidant and moderate antineurodegenerative potential, compared to the commercial variety. The highest contents of total flavonoids and individual compounds, such as arbutin and chlorogenic acid, as well as the strongest antioxidant and TYR inhibitory activities were reported for the ‘Takiša’ peel extract. The performed analyses have revealed that fruits of traditional Serbian pear varieties are rich in bioactive components and could be used as functional food and for possible nutraceutical applications, to prevent diseases induced by oxidative stress.
T2  - Polish Journal of Food and Nutrition Sciences
T1  - Phenolic Composition, and Antioxidant and Antineurodegenerative Potential of Methanolic Extracts of Fruit Peel and Flesh of Pear Varieties from Serbia
IS  - 2
VL  - 71
DO  - 10.31883/pjfns/138320
SP  - 225
EP  - 236
ER  - 

@article{
author = "Savić, Aleksandra and Alimpić Aradski, Ana and Živković, Jelena and Šavikin, Katarina and Jarić, Snežana and Marin, Petar and Duletić-Laušević, Sonja",
year = "2021",
abstract = "Pear (Pyrus communis L.) is consumed as fresh fruit, in numerous food products, and also used as a traditional remedy in various countries, including Serbia. In search of bioactive compounds, six traditional pear varieties (‘Vidovača’, ‘Lubeničarka’, ‘Karamanka’, ‘Jeribasma’, ‘Lončara’, ‘Takiša’) and wild pear from Serbia were investigated and compared with a commercial variety (‘Williams Bartlett’). The aim of this study was to determine the total phenolic and flavonoid contents, phenolic composition, antioxidant capacity, and antineurodegenerative activities of methanolic extracts of peel, flesh, and mixed peel and flesh of pear fruits. Phenolic composition of extracts was determined with HPLC-DAD, while the antioxidant activity of extracts was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), and ferric-reducing antioxidant power (FRAP) assays. Bearing in mind that oxidative stress is closely linked to neurodegeneration, the antineurodegenerative potential of the extracts was assessed by the inhibition of acetycholineserase (AChE) and tyrosinase (TYR) activities. The extracts of traditional varieties, particularly peel extracts, had a high content of phenolics, as well as significant antioxidant and moderate antineurodegenerative potential, compared to the commercial variety. The highest contents of total flavonoids and individual compounds, such as arbutin and chlorogenic acid, as well as the strongest antioxidant and TYR inhibitory activities were reported for the ‘Takiša’ peel extract. The performed analyses have revealed that fruits of traditional Serbian pear varieties are rich in bioactive components and could be used as functional food and for possible nutraceutical applications, to prevent diseases induced by oxidative stress.",
journal = "Polish Journal of Food and Nutrition Sciences",
title = "Phenolic Composition, and Antioxidant and Antineurodegenerative Potential of Methanolic Extracts of Fruit Peel and Flesh of Pear Varieties from Serbia",
number = "2",
volume = "71",
doi = "10.31883/pjfns/138320",
pages = "225-236"
}

Savić, A., Alimpić Aradski, A., Živković, J., Šavikin, K., Jarić, S., Marin, P.,& Duletić-Laušević, S.. (2021). Phenolic Composition, and Antioxidant and Antineurodegenerative Potential of Methanolic Extracts of Fruit Peel and Flesh of Pear Varieties from Serbia. in Polish Journal of Food and Nutrition Sciences, 71(2), 225-236.
https://doi.org/10.31883/pjfns/138320

Savić A, Alimpić Aradski A, Živković J, Šavikin K, Jarić S, Marin P, Duletić-Laušević S. Phenolic Composition, and Antioxidant and Antineurodegenerative Potential of Methanolic Extracts of Fruit Peel and Flesh of Pear Varieties from Serbia. in Polish Journal of Food and Nutrition Sciences. 2021;71(2):225-236.
doi:10.31883/pjfns/138320 .

Savić, Aleksandra, Alimpić Aradski, Ana, Živković, Jelena, Šavikin, Katarina, Jarić, Snežana, Marin, Petar, Duletić-Laušević, Sonja, "Phenolic Composition, and Antioxidant and Antineurodegenerative Potential of Methanolic Extracts of Fruit Peel and Flesh of Pear Varieties from Serbia" in Polish Journal of Food and Nutrition Sciences, 71, no. 2 (2021):225-236,
https://doi.org/10.31883/pjfns/138320 . .

TY  - JOUR
AU  - Oalđe Pavlović, Mariana
AU  - Kolarević, Stoimir
AU  - Đorđević, Jelena
AU  - Jovanović Marić, Jovana
AU  - Lunić, Tanja
AU  - Mandić, Marija
AU  - Kračun-Kolarević, Margareta
AU  - Živković, Jelena
AU  - Alimpić Aradski, Ana
AU  - Marin, Petar D.
AU  - Šavikin, Katarina
AU  - Vuković-Gačić, Branka
AU  - Božić Nedeljković, Biljana
AU  - Duletić-Laušević, Sonja
PY  - 2021
UR  - https://www.mdpi.com/2223-7747/10/11/2306
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4654
AB  - This study was designed to evaluate the genoprotective, antigenotoxic, as well as antitumor potential of methanolic, ethanolic, and aqueous extracts of Melissa officinalis, Mentha × piperita, Ocimum basilicum, Rosmarinus officinalis, Salvia officinalis, and Satureja montana (Lamiaceae), in different model systems. The polyphenols in these extracts were quantified both spectrophotometrically and using HPLC-DAD technique, while DPPH assay was used to assess the antioxidant activity. The genoprotective potential was tested on pUC19 Escherichia coli XL1-blue, and the antigenotoxicity on Salmonella typhimurium TA1535/pSK1002 and human lung fibroblasts, while the antitumor activity was assessed on colorectal cancer cells. Rosmarinic acid, quercetin, rutin, and luteolin-7-O-glucoside were among the identified compounds. Methanolic extracts had the best DPPH-scavenging and SOS-inducing activities, while ethanolic extracts exhibited the highest antigenotoxicity. Additionally, all extracts exhibited genoprotective potential on plasmid DNA. The antitumor effect was mediated by modulation of reactive oxygen species (ROS), nitric oxide (NO) production, and exhibition of genotoxic effects on tumor cells, especially with O. basilicum ethanolic extract. Generally, the investigated extracts were able to provide antioxidant protection for the acellular, prokaryotic, and normal human DNA, while also modulating the production of ROS and NO in tumor cells, leading to genotoxicity toward these cells and their decrease in proliferation.
PB  - Basel: MDPI
T2  - Plants
T1  - A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species
IS  - 11
VL  - 10
DO  - 10.3390/plants10112306
SP  - 2306
ER  - 

@article{
author = "Oalđe Pavlović, Mariana and Kolarević, Stoimir and Đorđević, Jelena and Jovanović Marić, Jovana and Lunić, Tanja and Mandić, Marija and Kračun-Kolarević, Margareta and Živković, Jelena and Alimpić Aradski, Ana and Marin, Petar D. and Šavikin, Katarina and Vuković-Gačić, Branka and Božić Nedeljković, Biljana and Duletić-Laušević, Sonja",
year = "2021",
abstract = "This study was designed to evaluate the genoprotective, antigenotoxic, as well as antitumor potential of methanolic, ethanolic, and aqueous extracts of Melissa officinalis, Mentha × piperita, Ocimum basilicum, Rosmarinus officinalis, Salvia officinalis, and Satureja montana (Lamiaceae), in different model systems. The polyphenols in these extracts were quantified both spectrophotometrically and using HPLC-DAD technique, while DPPH assay was used to assess the antioxidant activity. The genoprotective potential was tested on pUC19 Escherichia coli XL1-blue, and the antigenotoxicity on Salmonella typhimurium TA1535/pSK1002 and human lung fibroblasts, while the antitumor activity was assessed on colorectal cancer cells. Rosmarinic acid, quercetin, rutin, and luteolin-7-O-glucoside were among the identified compounds. Methanolic extracts had the best DPPH-scavenging and SOS-inducing activities, while ethanolic extracts exhibited the highest antigenotoxicity. Additionally, all extracts exhibited genoprotective potential on plasmid DNA. The antitumor effect was mediated by modulation of reactive oxygen species (ROS), nitric oxide (NO) production, and exhibition of genotoxic effects on tumor cells, especially with O. basilicum ethanolic extract. Generally, the investigated extracts were able to provide antioxidant protection for the acellular, prokaryotic, and normal human DNA, while also modulating the production of ROS and NO in tumor cells, leading to genotoxicity toward these cells and their decrease in proliferation.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species",
number = "11",
volume = "10",
doi = "10.3390/plants10112306",
pages = "2306"
}

Oalđe Pavlović, M., Kolarević, S., Đorđević, J., Jovanović Marić, J., Lunić, T., Mandić, M., Kračun-Kolarević, M., Živković, J., Alimpić Aradski, A., Marin, P. D., Šavikin, K., Vuković-Gačić, B., Božić Nedeljković, B.,& Duletić-Laušević, S.. (2021). A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species. in Plants
Basel: MDPI., 10(11), 2306.
https://doi.org/10.3390/plants10112306

Oalđe Pavlović M, Kolarević S, Đorđević J, Jovanović Marić J, Lunić T, Mandić M, Kračun-Kolarević M, Živković J, Alimpić Aradski A, Marin PD, Šavikin K, Vuković-Gačić B, Božić Nedeljković B, Duletić-Laušević S. A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species. in Plants. 2021;10(11):2306.
doi:10.3390/plants10112306 .

Oalđe Pavlović, Mariana, Kolarević, Stoimir, Đorđević, Jelena, Jovanović Marić, Jovana, Lunić, Tanja, Mandić, Marija, Kračun-Kolarević, Margareta, Živković, Jelena, Alimpić Aradski, Ana, Marin, Petar D., Šavikin, Katarina, Vuković-Gačić, Branka, Božić Nedeljković, Biljana, Duletić-Laušević, Sonja, "A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species" in Plants, 10, no. 11 (2021):2306,
https://doi.org/10.3390/plants10112306 . .

TY  - JOUR
AU  - Nikolić, Miloš
AU  - Marković, Tatjana
AU  - Marković, Dejan
AU  - Calhelha, Ricardo
AU  - Fernandes, Ângela
AU  - Ferreira, Isabel
AU  - Stojković, Dejan
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
PY  - 2021
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0352-51392100096N
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4761
AB  - Chemical composition of the essential oils (EO) of Pelargonium graveolens, Leptospermum petersonii and Cymbopogon martinii var. motia, and the absolute of Rosa centifolia and their bioactivity were examined. Major compounds in P. graveolens EO were monoterpene alcohols citronellol, geraniol and linalool; in L. petersonii EO monoterpene aldehydes geranial, neral and citronellal; in C. martiniii var. motia EO monoterpene alcohol geraniol and ester geranyl acetate, while in absolute of R. centifolia aromatic alcohol 2-phenylethanol. The EO of L. petersonii showed the strongest antibacterial while the EO of C. martinii var. motia the strongest antifungal potential. The best biofilm inhibition capacity was observed with R. centifolia absolute. The results of scanning electron microscopy analysis indicated that the EOs of L. petersonii and P. graveolens changed the number and morphology of C. albicans cells. The L. petersonii EO was the most potent toward tumour cells and exhibited the best biological activity. This is first comparative report summarizing efficacy of studied aromatic samples against pathogenic microbes, providing deeper insight into the modes of antimicrobial action, and at the same time describing their cytotoxicity against cell lines.
PB  - Belgrade: Serbian Chemical Socitey
T2  - Journal of the Serbian Chemical Society
T1  - Chemical composition and biological properties of Pelargonium graveolens, Leptospermum petersonii and Cymbopogon martinii var. motia essential oils and of Rosa centifolia absolute
IS  - 12
VL  - 86
DO  - 10.2298/JSC210729096N
SP  - 1291
EP  - 1303
ER  - 

@article{
author = "Nikolić, Miloš and Marković, Tatjana and Marković, Dejan and Calhelha, Ricardo and Fernandes, Ângela and Ferreira, Isabel and Stojković, Dejan and Ćirić, Ana and Glamočlija, Jasmina and Soković, Marina",
year = "2021",
abstract = "Chemical composition of the essential oils (EO) of Pelargonium graveolens, Leptospermum petersonii and Cymbopogon martinii var. motia, and the absolute of Rosa centifolia and their bioactivity were examined. Major compounds in P. graveolens EO were monoterpene alcohols citronellol, geraniol and linalool; in L. petersonii EO monoterpene aldehydes geranial, neral and citronellal; in C. martiniii var. motia EO monoterpene alcohol geraniol and ester geranyl acetate, while in absolute of R. centifolia aromatic alcohol 2-phenylethanol. The EO of L. petersonii showed the strongest antibacterial while the EO of C. martinii var. motia the strongest antifungal potential. The best biofilm inhibition capacity was observed with R. centifolia absolute. The results of scanning electron microscopy analysis indicated that the EOs of L. petersonii and P. graveolens changed the number and morphology of C. albicans cells. The L. petersonii EO was the most potent toward tumour cells and exhibited the best biological activity. This is first comparative report summarizing efficacy of studied aromatic samples against pathogenic microbes, providing deeper insight into the modes of antimicrobial action, and at the same time describing their cytotoxicity against cell lines.",
publisher = "Belgrade: Serbian Chemical Socitey",
journal = "Journal of the Serbian Chemical Society",
title = "Chemical composition and biological properties of Pelargonium graveolens, Leptospermum petersonii and Cymbopogon martinii var. motia essential oils and of Rosa centifolia absolute",
number = "12",
volume = "86",
doi = "10.2298/JSC210729096N",
pages = "1291-1303"
}

Nikolić, M., Marković, T., Marković, D., Calhelha, R., Fernandes, Â., Ferreira, I., Stojković, D., Ćirić, A., Glamočlija, J.,& Soković, M.. (2021). Chemical composition and biological properties of Pelargonium graveolens, Leptospermum petersonii and Cymbopogon martinii var. motia essential oils and of Rosa centifolia absolute. in Journal of the Serbian Chemical Society
Belgrade: Serbian Chemical Socitey., 86(12), 1291-1303.
https://doi.org/10.2298/JSC210729096N

Nikolić M, Marković T, Marković D, Calhelha R, Fernandes Â, Ferreira I, Stojković D, Ćirić A, Glamočlija J, Soković M. Chemical composition and biological properties of Pelargonium graveolens, Leptospermum petersonii and Cymbopogon martinii var. motia essential oils and of Rosa centifolia absolute. in Journal of the Serbian Chemical Society. 2021;86(12):1291-1303.
doi:10.2298/JSC210729096N .

Nikolić, Miloš, Marković, Tatjana, Marković, Dejan, Calhelha, Ricardo, Fernandes, Ângela, Ferreira, Isabel, Stojković, Dejan, Ćirić, Ana, Glamočlija, Jasmina, Soković, Marina, "Chemical composition and biological properties of Pelargonium graveolens, Leptospermum petersonii and Cymbopogon martinii var. motia essential oils and of Rosa centifolia absolute" in Journal of the Serbian Chemical Society, 86, no. 12 (2021):1291-1303,
https://doi.org/10.2298/JSC210729096N . .

TY  - JOUR
AU  - Mićanović, Dragica
AU  - Saksida, Tamara
AU  - Koprivica, Ivan
AU  - Šenerović, Lidija
AU  - Morić, Ivana
AU  - Šavikin, Katarina
AU  - Menković, Nebojša
AU  - Pejnović, Nada
AU  - Stojanović, Ivana D.
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3834
AB  - Chokeberry (Aronia melanocarpa) fruit extracts (CE) are rich in polyphenols and usually exhibit immunomodulatory, anti-viral and anti-bacterial effects. We have previously shown that the CE used in this study activated macrophages and stimulated effector T cell differentiation in vitro. When applied orally to healthy mice, CE increased the proportion of CD11c+ dendritic cells in the gut-associated lymphoid tissue. CE-pretreated BALB/c mice readily eradicated orally ingested Listeria monocytogenes as evidenced by a slighter decrease in body weight and number of bacteria recovered from the spleen and reduced spleen size compared to the control infected mice. CE pretreatment in infected mice resulted in higher proportions of CD11b+ macrophages and CD8+ cytotoxic T cells both in the gut and the spleen. Phagocytosis, reactive oxygen species production and the proportions of activated CD86+ macrophages (CD11b+) and dendritic cells (CD11c+) was also enhanced in CE-pretreated infected mice. Further, the expression of inducible nitric oxide synthase and IL-6 was increased in CE-pretreated infected mice and the similar results were obtained in peritoneal macrophages in vitro. This effect of CE was associated with increased phosphorylation of IκB and Notch1 production. Finally, CE pretreatment elevated the proportion of perforin-producing cells in the spleen compared to control infected mice. This study demonstrates that prophylactic treatment with CE leads to more rapid eradication of bacterial infection with Listeria monocytogenes predominantly through increased activity of myeloid cells in the gut and in the spleen.
PB  - United Kingdom : Royal Society of Chemistry
T2  - Food and Function
T1  - Immunomodulatory activity and protective effects of chokeberry fruit extract on Listeria monocytogenes infection in mice
IS  - 9
VL  - 11
DO  - 10.1039/D0FO00946F
SP  - 7793
EP  - 7803
ER  - 

@article{
author = "Mićanović, Dragica and Saksida, Tamara and Koprivica, Ivan and Šenerović, Lidija and Morić, Ivana and Šavikin, Katarina and Menković, Nebojša and Pejnović, Nada and Stojanović, Ivana D.",
year = "2020",
abstract = "Chokeberry (Aronia melanocarpa) fruit extracts (CE) are rich in polyphenols and usually exhibit immunomodulatory, anti-viral and anti-bacterial effects. We have previously shown that the CE used in this study activated macrophages and stimulated effector T cell differentiation in vitro. When applied orally to healthy mice, CE increased the proportion of CD11c+ dendritic cells in the gut-associated lymphoid tissue. CE-pretreated BALB/c mice readily eradicated orally ingested Listeria monocytogenes as evidenced by a slighter decrease in body weight and number of bacteria recovered from the spleen and reduced spleen size compared to the control infected mice. CE pretreatment in infected mice resulted in higher proportions of CD11b+ macrophages and CD8+ cytotoxic T cells both in the gut and the spleen. Phagocytosis, reactive oxygen species production and the proportions of activated CD86+ macrophages (CD11b+) and dendritic cells (CD11c+) was also enhanced in CE-pretreated infected mice. Further, the expression of inducible nitric oxide synthase and IL-6 was increased in CE-pretreated infected mice and the similar results were obtained in peritoneal macrophages in vitro. This effect of CE was associated with increased phosphorylation of IκB and Notch1 production. Finally, CE pretreatment elevated the proportion of perforin-producing cells in the spleen compared to control infected mice. This study demonstrates that prophylactic treatment with CE leads to more rapid eradication of bacterial infection with Listeria monocytogenes predominantly through increased activity of myeloid cells in the gut and in the spleen.",
publisher = "United Kingdom : Royal Society of Chemistry",
journal = "Food and Function",
title = "Immunomodulatory activity and protective effects of chokeberry fruit extract on Listeria monocytogenes infection in mice",
number = "9",
volume = "11",
doi = "10.1039/D0FO00946F",
pages = "7793-7803"
}

Mićanović, D., Saksida, T., Koprivica, I., Šenerović, L., Morić, I., Šavikin, K., Menković, N., Pejnović, N.,& Stojanović, I. D.. (2020). Immunomodulatory activity and protective effects of chokeberry fruit extract on Listeria monocytogenes infection in mice. in Food and Function
United Kingdom : Royal Society of Chemistry., 11(9), 7793-7803.
https://doi.org/10.1039/D0FO00946F

Mićanović D, Saksida T, Koprivica I, Šenerović L, Morić I, Šavikin K, Menković N, Pejnović N, Stojanović ID. Immunomodulatory activity and protective effects of chokeberry fruit extract on Listeria monocytogenes infection in mice. in Food and Function. 2020;11(9):7793-7803.
doi:10.1039/D0FO00946F .

Mićanović, Dragica, Saksida, Tamara, Koprivica, Ivan, Šenerović, Lidija, Morić, Ivana, Šavikin, Katarina, Menković, Nebojša, Pejnović, Nada, Stojanović, Ivana D., "Immunomodulatory activity and protective effects of chokeberry fruit extract on Listeria monocytogenes infection in mice" in Food and Function, 11, no. 9 (2020):7793-7803,
https://doi.org/10.1039/D0FO00946F . .

TY  - JOUR
AU  - Oalđe, Mariana M.
AU  - Kolarević, Stoimir
AU  - Živković, Jelena C.
AU  - Vuković-Gačić, Branka S.
AU  - Jovanović Marić, Jovana
AU  - Kračun-Kolarević, Margareta
AU  - Đorđević, Jelena Z.
AU  - Alimpić Aradski, Ana Z.
AU  - Marin, Petar D.
AU  - Šavikin, Katarina P.
AU  - Duletić-Laušević, Sonja N.
PY  - 2020
UR  - https://linkinghub.elsevier.com/retrieve/pii/S1319016420302413
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4001
AB  - The main objective of this research was to evaluate the impact of methanolic, ethanolic and aqueous extracts of Origanum majorana L., Origanum vulgare L., Teucrium chamaedrys L., Teucrium montanum L., Thymus serpyllum L. and Thymus vulgaris L. (Lamiaceae) on the effects of free radicals using different model systems. The extracts were characterized on the basis of the contents of total phenolics, phenolic acids, flavonoids and flavonols, and also using high-performance liquid chromatography with diode-array detection. Antioxidant activity in vitro was assessed using DPPH assay. The genoprotective properties were tested using plasmid relaxation assay on pUC19 E. coli XL1-Blue, while SOS/umuC assay on Salmonella typhimurium TA1535/pSK1002 and Comet assay on human lung fibroblasts were used to assess the antigenotoxicity of the extracts. Ethanolic extracts had the most phenolics (up to 236.20 mg GAE/g at 0.5 mg/mL), flavonoids (up to 42.47 mg QE/g at 0.5 mg/mL) and flavonols (up to 16.56 mg QE/g at 0.5 mg/mL), and they exhibited the highest DPPH activity (up to 92.16% at 0.25 mg/mL). Interestingly enough, aqueous extracts provided the best protection of plasmid DNA (the lowest IC50 value was 0.17 mg/mL). Methanolic extracts, on the other hand, most efficiently protected the prokaryotic DNA, while all the extracts had a significant impact against genomic damages inflicted on human fibroblasts. O. vulgare extracts are considered to be the most promising in preserving the overall DNA integrity against oxidative genomic damages. Moreover, HPLC-DAD analysis highlighted rosmarinic acid as the most abundant in the investigated samples (551.45 mg/mL in total in all the extracts), followed by luteolin-7-O-glucoside (150.19 mg/mL in total), while their presence correlates with most of the displayed activities. The novelty of this study is reflected in the application of a prokaryotic model for testing the antigenotoxic effects of Lamiaceae species, as no previous reports have yet been published on the genoprotective potential of these species.
PB  - Elsevier BV
T2  - Saudi Pharmaceutical Journal
T1  - The impact of different extracts of six Lamiaceae species on deleterious effects of oxidative stress assessed in acellular, prokaryotic and eukaryotic models in vitro
DO  - 10.1016/j.jsps.2020.10.006
ER  - 

@article{
author = "Oalđe, Mariana M. and Kolarević, Stoimir and Živković, Jelena C. and Vuković-Gačić, Branka S. and Jovanović Marić, Jovana and Kračun-Kolarević, Margareta and Đorđević, Jelena Z. and Alimpić Aradski, Ana Z. and Marin, Petar D. and Šavikin, Katarina P. and Duletić-Laušević, Sonja N.",
year = "2020",
abstract = "The main objective of this research was to evaluate the impact of methanolic, ethanolic and aqueous extracts of Origanum majorana L., Origanum vulgare L., Teucrium chamaedrys L., Teucrium montanum L., Thymus serpyllum L. and Thymus vulgaris L. (Lamiaceae) on the effects of free radicals using different model systems. The extracts were characterized on the basis of the contents of total phenolics, phenolic acids, flavonoids and flavonols, and also using high-performance liquid chromatography with diode-array detection. Antioxidant activity in vitro was assessed using DPPH assay. The genoprotective properties were tested using plasmid relaxation assay on pUC19 E. coli XL1-Blue, while SOS/umuC assay on Salmonella typhimurium TA1535/pSK1002 and Comet assay on human lung fibroblasts were used to assess the antigenotoxicity of the extracts. Ethanolic extracts had the most phenolics (up to 236.20 mg GAE/g at 0.5 mg/mL), flavonoids (up to 42.47 mg QE/g at 0.5 mg/mL) and flavonols (up to 16.56 mg QE/g at 0.5 mg/mL), and they exhibited the highest DPPH activity (up to 92.16% at 0.25 mg/mL). Interestingly enough, aqueous extracts provided the best protection of plasmid DNA (the lowest IC50 value was 0.17 mg/mL). Methanolic extracts, on the other hand, most efficiently protected the prokaryotic DNA, while all the extracts had a significant impact against genomic damages inflicted on human fibroblasts. O. vulgare extracts are considered to be the most promising in preserving the overall DNA integrity against oxidative genomic damages. Moreover, HPLC-DAD analysis highlighted rosmarinic acid as the most abundant in the investigated samples (551.45 mg/mL in total in all the extracts), followed by luteolin-7-O-glucoside (150.19 mg/mL in total), while their presence correlates with most of the displayed activities. The novelty of this study is reflected in the application of a prokaryotic model for testing the antigenotoxic effects of Lamiaceae species, as no previous reports have yet been published on the genoprotective potential of these species.",
publisher = "Elsevier BV",
journal = "Saudi Pharmaceutical Journal",
title = "The impact of different extracts of six Lamiaceae species on deleterious effects of oxidative stress assessed in acellular, prokaryotic and eukaryotic models in vitro",
doi = "10.1016/j.jsps.2020.10.006"
}

Oalđe, M. M., Kolarević, S., Živković, J. C., Vuković-Gačić, B. S., Jovanović Marić, J., Kračun-Kolarević, M., Đorđević, J. Z., Alimpić Aradski, A. Z., Marin, P. D., Šavikin, K. P.,& Duletić-Laušević, S. N.. (2020). The impact of different extracts of six Lamiaceae species on deleterious effects of oxidative stress assessed in acellular, prokaryotic and eukaryotic models in vitro. in Saudi Pharmaceutical Journal
Elsevier BV..
https://doi.org/10.1016/j.jsps.2020.10.006

Oalđe MM, Kolarević S, Živković JC, Vuković-Gačić BS, Jovanović Marić J, Kračun-Kolarević M, Đorđević JZ, Alimpić Aradski AZ, Marin PD, Šavikin KP, Duletić-Laušević SN. The impact of different extracts of six Lamiaceae species on deleterious effects of oxidative stress assessed in acellular, prokaryotic and eukaryotic models in vitro. in Saudi Pharmaceutical Journal. 2020;.
doi:10.1016/j.jsps.2020.10.006 .

Oalđe, Mariana M., Kolarević, Stoimir, Živković, Jelena C., Vuković-Gačić, Branka S., Jovanović Marić, Jovana, Kračun-Kolarević, Margareta, Đorđević, Jelena Z., Alimpić Aradski, Ana Z., Marin, Petar D., Šavikin, Katarina P., Duletić-Laušević, Sonja N., "The impact of different extracts of six Lamiaceae species on deleterious effects of oxidative stress assessed in acellular, prokaryotic and eukaryotic models in vitro" in Saudi Pharmaceutical Journal (2020),
https://doi.org/10.1016/j.jsps.2020.10.006 . .

TY  - JOUR
AU  - Živković, Jelena
AU  - Ilić, Milan
AU  - Šavikin, Katarina
AU  - Zdunić, Gordana
AU  - Ilić, Aleksandra
AU  - Stojković, Dejan
PY  - 2020
UR  - https://www.frontiersin.org/article/10.3389/fphar.2020.01020/full
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32733251
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC7360817
UR  - https://radar.ibiss.bg.ac.rs/123456789/3849
AB  - Balkan Peninsula is one of the most important biodiversity centers in Europe. Despite that, the usage of plant species in the traditional medicine of some Balkan regions remained largely unexplored in the past. This study aimed to collect and document data on the traditional use of medicinal plants in Pčinja district in South-Eastern Serbia, which is among the least developed regions in Serbia. Also, comparison with data collected by Dr. Jovan Tucakov, in a book called Herbal therapy was conducted. The survey was carried out using semi-structured interviews and 113 informants were interviewed. Quantitative ethnobotany factors were calculated, allowing us discussing the results. The informants reported data on 86 medicinal plants belonging to 43 families in Pčinja district. Lamiaceae, Asteraceae, and Rosaceae were the dominant locally used families. The species with the highest number of use reports were Mentha piperita, Matricaria chamomilla, and Hypericum perforatum. Gastrointestinal ailments, respiratory problems and skin diseases were the most frequently reported indications. Usually, the administration was primarily oral followed by topical applications. Leaves were dominantly exploited plant parts and the most frequent preparation form was infusion. Medicinal plants in Pčinja district are mainly used as a mode of primary health care for treating minor health issues. After comparing our results with the ones collected half a century ago by Dr. Jovan Tucakov we can conclude that plant species mentioned in our investigation previously had a much wider spectrum of application.
PB  - Frontiers Media S.A.
T2  - Frontiers in Pharmacology
T1  - Traditional Use of Medicinal Plants in South-Eastern Serbia (Pčinja District): Ethnopharmacological Investigation on the Current Status and Comparison With Half a Century Old Data.
VL  - 11
DO  - 10.3389/fphar.2020.01020
SP  - 1020
ER  - 

@article{
author = "Živković, Jelena and Ilić, Milan and Šavikin, Katarina and Zdunić, Gordana and Ilić, Aleksandra and Stojković, Dejan",
year = "2020",
abstract = "Balkan Peninsula is one of the most important biodiversity centers in Europe. Despite that, the usage of plant species in the traditional medicine of some Balkan regions remained largely unexplored in the past. This study aimed to collect and document data on the traditional use of medicinal plants in Pčinja district in South-Eastern Serbia, which is among the least developed regions in Serbia. Also, comparison with data collected by Dr. Jovan Tucakov, in a book called Herbal therapy was conducted. The survey was carried out using semi-structured interviews and 113 informants were interviewed. Quantitative ethnobotany factors were calculated, allowing us discussing the results. The informants reported data on 86 medicinal plants belonging to 43 families in Pčinja district. Lamiaceae, Asteraceae, and Rosaceae were the dominant locally used families. The species with the highest number of use reports were Mentha piperita, Matricaria chamomilla, and Hypericum perforatum. Gastrointestinal ailments, respiratory problems and skin diseases were the most frequently reported indications. Usually, the administration was primarily oral followed by topical applications. Leaves were dominantly exploited plant parts and the most frequent preparation form was infusion. Medicinal plants in Pčinja district are mainly used as a mode of primary health care for treating minor health issues. After comparing our results with the ones collected half a century ago by Dr. Jovan Tucakov we can conclude that plant species mentioned in our investigation previously had a much wider spectrum of application.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Pharmacology",
title = "Traditional Use of Medicinal Plants in South-Eastern Serbia (Pčinja District): Ethnopharmacological Investigation on the Current Status and Comparison With Half a Century Old Data.",
volume = "11",
doi = "10.3389/fphar.2020.01020",
pages = "1020"
}

Živković, J., Ilić, M., Šavikin, K., Zdunić, G., Ilić, A.,& Stojković, D.. (2020). Traditional Use of Medicinal Plants in South-Eastern Serbia (Pčinja District): Ethnopharmacological Investigation on the Current Status and Comparison With Half a Century Old Data.. in Frontiers in Pharmacology
Frontiers Media S.A.., 11, 1020.
https://doi.org/10.3389/fphar.2020.01020

Živković J, Ilić M, Šavikin K, Zdunić G, Ilić A, Stojković D. Traditional Use of Medicinal Plants in South-Eastern Serbia (Pčinja District): Ethnopharmacological Investigation on the Current Status and Comparison With Half a Century Old Data.. in Frontiers in Pharmacology. 2020;11:1020.
doi:10.3389/fphar.2020.01020 .

Živković, Jelena, Ilić, Milan, Šavikin, Katarina, Zdunić, Gordana, Ilić, Aleksandra, Stojković, Dejan, "Traditional Use of Medicinal Plants in South-Eastern Serbia (Pčinja District): Ethnopharmacological Investigation on the Current Status and Comparison With Half a Century Old Data." in Frontiers in Pharmacology, 11 (2020):1020,
https://doi.org/10.3389/fphar.2020.01020 . .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Jevremović, Stojan
AU  - Kostić, Igor
AU  - Kostić, Miroslav
AU  - Vuleta, Ana
AU  - Manitašević Jovanović, Sanja
AU  - Šešlija Jovanović, Darka
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3837
AB  - The bean weevil Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae) can cause significant losses in production of its primary host common bean Phaseolus vulgaris L. To avoid bean protection with environmentally risky chemical insecticides and provide sustainable and safe production of food, new pest management methods based on natural compounds are investigated. In the present study, we evaluated protective potential of the essential oil (EO) from the common thyme Thymus vulgaris L. applied on bean seeds. We assessed residual contact toxicity of thyme EO and its effects on A. obtectus longevity, oviposition and adult emergence. Furthermore, to elucidate the role of oxidative stress in thyme EO toxicity, we estimated the levels of oxidatively damaged proteins and lipids, as well as the level of thiols which have important role for antioxidant capacity. We found that thyme oil significantly reduced adult survival and longevity, induced oxidative damage to lipids and proteins and depleted protein and non-protein thiols in a concentration-dependent manner. Females appeared to be more tolerant to thyme oil treatment than males. Sublethal EO concentrations affected oxidative stress indices, deterred oviposition and strongly inhibited adult emergence. The results suggest that thyme oil has the potential to be used as an ecofriendly insecticide for A. obtectus control.
PB  - Multidisciplinary Digital Publishing Institute
T2  - Insects
T1  - Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus
IS  - 9
VL  - 11
DO  - 10.3390/insects11090563
SP  - 563
ER  - 

@article{
author = "Lazarević, Jelica and Jevremović, Stojan and Kostić, Igor and Kostić, Miroslav and Vuleta, Ana and Manitašević Jovanović, Sanja and Šešlija Jovanović, Darka",
year = "2020",
abstract = "The bean weevil Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae) can cause significant losses in production of its primary host common bean Phaseolus vulgaris L. To avoid bean protection with environmentally risky chemical insecticides and provide sustainable and safe production of food, new pest management methods based on natural compounds are investigated. In the present study, we evaluated protective potential of the essential oil (EO) from the common thyme Thymus vulgaris L. applied on bean seeds. We assessed residual contact toxicity of thyme EO and its effects on A. obtectus longevity, oviposition and adult emergence. Furthermore, to elucidate the role of oxidative stress in thyme EO toxicity, we estimated the levels of oxidatively damaged proteins and lipids, as well as the level of thiols which have important role for antioxidant capacity. We found that thyme oil significantly reduced adult survival and longevity, induced oxidative damage to lipids and proteins and depleted protein and non-protein thiols in a concentration-dependent manner. Females appeared to be more tolerant to thyme oil treatment than males. Sublethal EO concentrations affected oxidative stress indices, deterred oviposition and strongly inhibited adult emergence. The results suggest that thyme oil has the potential to be used as an ecofriendly insecticide for A. obtectus control.",
publisher = "Multidisciplinary Digital Publishing Institute",
journal = "Insects",
title = "Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus",
number = "9",
volume = "11",
doi = "10.3390/insects11090563",
pages = "563"
}

Lazarević, J., Jevremović, S., Kostić, I., Kostić, M., Vuleta, A., Manitašević Jovanović, S.,& Šešlija Jovanović, D.. (2020). Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus. in Insects
Multidisciplinary Digital Publishing Institute., 11(9), 563.
https://doi.org/10.3390/insects11090563

Lazarević J, Jevremović S, Kostić I, Kostić M, Vuleta A, Manitašević Jovanović S, Šešlija Jovanović D. Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus. in Insects. 2020;11(9):563.
doi:10.3390/insects11090563 .

Lazarević, Jelica, Jevremović, Stojan, Kostić, Igor, Kostić, Miroslav, Vuleta, Ana, Manitašević Jovanović, Sanja, Šešlija Jovanović, Darka, "Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus" in Insects, 11, no. 9 (2020):563,
https://doi.org/10.3390/insects11090563 . .