Enzymatic biomarkers as indicators of dietary cadmium in gypsy moth caterpillars
2013
Аутори:
Vlahović, MilenaPerić Mataruga, Vesna
Mrdaković, Marija
Matić, Dragana
Lazarević, Jelica
Nenadović, Vera
Ilijin, Larisa
Тип документа:
Чланак у часопису (Објављена верзија)
,
2012, Springer-Verlag Berlin Heidelberg
Метаподаци
Приказ свих података о документуАпстракт:
Heavy metals damage the structure, chemistry, and function of cells, including enzyme systems inside them. Variation in the profile of biochemical biomarkers in prevalent species should be used for assessing environmental contamination. The present study pays attention to the phosphatases present in the midgut of gypsy moth fourth instar caterpillars, which had been exposed to short- and long-term cadmium intake at 10 and 30 μg Cd/g dry food. Chronic cadmium ingestion significantly inhibited the activity of all examined phosphatases, while only the activity of lysosomal phosphatase was acutely decreased. Total acid phosphatase activity recovered from both long-term cadmium treatments within 3 days. The low index of phenotypic plasticity was connected to high variability of plasticity. Dependence of phosphatase isoforms on genotype and duration of cadmium treatment was determined. We concluded that, with further investigations, profiling of total acid phosphatase activity, as well as the lysosomal fraction can be used as a biomarker for acute sublethal metal toxicity.
Кључне речи:
Gypsy moth; Cadmium; Phosphatases; Acute and chronic toxicity; Enzyme variability; BiomarkersИзвор:
Environmental Science and Pollution Research, 2013, 20, 5, 3447-3455Финансирање / пројекти:
- Утицај магнетних поља и других срединских стресора на физиолошке одговоре и понашање различитих врста (RS-MESTD-Basic Research (BR or ON)-173027)
DOI: 10.1007/s11356-012-1292-3
ISSN: 0944-1344
PubMed: 23143823