dc.creator | Krizak, Strahinja | |
dc.creator | Nikoic, Ljiljana | |
dc.creator | Stanic, Marina | |
dc.creator | Zizic, Milan | |
dc.creator | Zakrzewska, Joanna | |
dc.creator | Zivic, Miroslav | |
dc.creator | Todorović, Nataša | |
dc.date.accessioned | 2016-05-23T10:59:48Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 1769-7123 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/1977 | |
dc.description.abstract | We describe here whole-cell currents of droplets prepared from the
apical region of growing Phycomyces blakesleeanus sporangiophores.
Whole-cell current recordings revealed the osmotically activated,
outwardly rectifying, fast inactivating instantaneous current (ORIC)
with biophysical properties closely resembling volume-regulated anionic
current (VRAC). ORIC is activated under conditions of osmotically
induced swelling and shows strong selectivity for anions over cations.
In addition, ORIC shows voltage and time-dependent inactivation at
positive potentials and recovery from inactivation at negative
potentials. ORIC is blocked by anthracene-9-carboxylic acid, an anion
channel blocker, in a voltage-dependent manner. This is the first report
of the presence of VRAC-like current in an organism outside the chordate
lineage. (C) 2015 Institut Pasteur. Published by Elsevier Masson SAS.
All rights reserved. | en |
dc.description.sponsorship | Ministry of Education, Science and Technological Development of the
Republic of Serbia {[}173040] | |
dc.language | English | |
dc.rights | restrictedAccess | |
dc.source | Research in Microbiology | |
dc.subject | Filamentous fungi | |
dc.subject | Whole-cell patch-clamp | |
dc.subject | Outward rectifier | |
dc.subject | Voltage-dependent inactivation | |
dc.title | Osmotic swelling activates a novel anionic current with VRAC-like
properties in a cytoplasmic droplet membrane from Phycomyces
blakesleeanus sporangiophores | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Кризак, Страхиња; Никоиц, Љиљана; Закрзеwска, Јоанна; Тодоровиц, Натаса; Станиц, Марина; Зивиц, Мирослав; Зизиц, Милан; | |
dc.citation.issue | 3 | |
dc.citation.volume | 166 | |
dc.identifier.doi | 10.1016/j.resmic.2015.02.004 | |
dc.identifier.scopus | 2-s2.0-84926407109 | |
dc.identifier.wos | 000353600700005 | |
dc.citation.spage | 162 | |
dc.citation.epage | 173 | |
dc.type.version | publishedVersion | en |