dc.creator | Zogović, Nevena | |
dc.creator | Tovilović-Kovačević, Gordana | |
dc.creator | Misirkić Marjanović, Maja | |
dc.creator | Vučićević, Ljubica | |
dc.creator | Janjetović, Kristina | |
dc.creator | Harhaji-Trajković, Ljubica | |
dc.creator | Trajkovic, Vladimir | |
dc.date.accessioned | 2016-05-23T10:59:49Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 1471-4159 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/1978 | |
dc.description.abstract | We explored the interplay between the intracellular energy sensor
AMP-activated protein kinase (AMPK), extracellular signal-regulated
kinase (ERK), and autophagy in phorbol myristate acetate (PMA)-induced
neuronal differentiation of SH-SY5Y human neuroblastoma cells.
PMA-triggered expression of neuronal markers (dopamine transporter,
microtubule-associated protein 2, -tubulin) was associated with an
autophagic response, measured by the conversion of
microtubule-associated protein light chain 3 (LC3)-I to
autophagosome-bound LC3-II, increase in autophagic flux, and expression
of autophagy-related (Atg) proteins Atg7 and beclin-1. This coincided
with the transient activation of AMPK and sustained activation of ERK.
Pharmacological inhibition or RNA interference-mediated silencing of
AMPK suppressed PMA-induced expression of neuronal markers, as well as
ERK activation and autophagy. A selective pharmacological blockade of
ERK prevented PMA-induced neuronal differentiation and autophagy
induction without affecting AMPK phosphorylation. Conversely, the
inhibition of autophagy downstream of AMPK/ERK, either by
pharmacological agents or LC3 knockdown, promoted the expression of
neuronal markers, thus indicating a role of autophagy in the suppression
of PMA-induced differentiation of SH-SY5Y cells. Therefore, PMA-induced
neuronal differentiation of SH-SY5Y cells depends on a complex interplay
between AMPK, ERK, and autophagy, in which the stimulatory effects of
AMPK/ERK signaling are counteracted by the coinciding autophagic
response. | en |
dc.description.sponsorship | Ministry of Education, Science and Technological Development of the
Republic of Serbia {[}41025, 173053] | |
dc.language | English | |
dc.rights | restrictedAccess | |
dc.source | Journal of Neurochemistry | |
dc.subject | autophagy | |
dc.subject | extracellular signal-regulated
kinase | |
dc.subject | neuronal differentiation | |
dc.subject | phorbol myristate acetate | |
dc.title | Coordinated activation of AMP-activated protein kinase, extracellular
signal-regulated kinase, and autophagy regulates phorbol myristate
acetate-induced differentiation of SH-SY5Y neuroblastoma cells | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Вуцицевиц, Љубица; Трајковиц, Владимир; Хархаји-Трајковић, Љубица М.; Јањетовиц, Кристина; Мисиркиц-Марјановиц, Маја; Зоговиц, Невена; Товиловиц-Ковацевиц, Гордана; | |
dc.citation.issue | 2 | |
dc.citation.volume | 133 | |
dc.identifier.doi | 10.1111/jnc.12980 | |
dc.identifier.scopus | 2-s2.0-84926670505 | |
dc.identifier.wos | 000352796000007 | |
dc.citation.spage | 223 | |
dc.citation.epage | 232 | |
dc.type.version | publishedVersion | en |