Prikaz osnovnih podataka o dokumentu
Coordinated activation of AMP-activated protein kinase, extracellular signal-regulated kinase, and autophagy regulates phorbol myristate acetate-induced differentiation of SH-SY5Y neuroblastoma cells
dc.creator | Zogović, Nevena | |
dc.creator | Tovilović-Kovačević, Gordana | |
dc.creator | Misirkić Marjanović, Maja | |
dc.creator | Vučićević, Ljubica | |
dc.creator | Janjetović, Kristina | |
dc.creator | Harhaji-Trajković, Ljubica | |
dc.creator | Trajkovic, Vladimir | |
dc.date.accessioned | 2016-05-23T10:59:49Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 1471-4159 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/1978 | |
dc.description.abstract | We explored the interplay between the intracellular energy sensor AMP-activated protein kinase (AMPK), extracellular signal-regulated kinase (ERK), and autophagy in phorbol myristate acetate (PMA)-induced neuronal differentiation of SH-SY5Y human neuroblastoma cells. PMA-triggered expression of neuronal markers (dopamine transporter, microtubule-associated protein 2, -tubulin) was associated with an autophagic response, measured by the conversion of microtubule-associated protein light chain 3 (LC3)-I to autophagosome-bound LC3-II, increase in autophagic flux, and expression of autophagy-related (Atg) proteins Atg7 and beclin-1. This coincided with the transient activation of AMPK and sustained activation of ERK. Pharmacological inhibition or RNA interference-mediated silencing of AMPK suppressed PMA-induced expression of neuronal markers, as well as ERK activation and autophagy. A selective pharmacological blockade of ERK prevented PMA-induced neuronal differentiation and autophagy induction without affecting AMPK phosphorylation. Conversely, the inhibition of autophagy downstream of AMPK/ERK, either by pharmacological agents or LC3 knockdown, promoted the expression of neuronal markers, thus indicating a role of autophagy in the suppression of PMA-induced differentiation of SH-SY5Y cells. Therefore, PMA-induced neuronal differentiation of SH-SY5Y cells depends on a complex interplay between AMPK, ERK, and autophagy, in which the stimulatory effects of AMPK/ERK signaling are counteracted by the coinciding autophagic response. | en |
dc.description.sponsorship | Ministry of Education, Science and Technological Development of the Republic of Serbia {[}41025, 173053] | |
dc.language | English | |
dc.rights | restrictedAccess | |
dc.source | Journal of Neurochemistry | |
dc.subject | autophagy | |
dc.subject | extracellular signal-regulated kinase | |
dc.subject | neuronal differentiation | |
dc.subject | phorbol myristate acetate | |
dc.title | Coordinated activation of AMP-activated protein kinase, extracellular signal-regulated kinase, and autophagy regulates phorbol myristate acetate-induced differentiation of SH-SY5Y neuroblastoma cells | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Вуцицевиц, Љубица; Трајковиц, Владимир; Хархаји-Трајковић, Љубица М.; Јањетовиц, Кристина; Мисиркиц-Марјановиц, Маја; Зоговиц, Невена; Товиловиц-Ковацевиц, Гордана; | |
dc.citation.issue | 2 | |
dc.citation.volume | 133 | |
dc.identifier.doi | 10.1111/jnc.12980 | |
dc.identifier.scopus | 2-s2.0-84926670505 | |
dc.identifier.wos | 000352796000007 | |
dc.citation.spage | 223 | |
dc.citation.epage | 232 | |
dc.type.version | publishedVersion | en |
Dokumenti
Datoteke | Veličina | Format | Pregled |
---|---|---|---|
Uz ovaj zapis nema datoteka. |