dc.creator | Laketa, Danijela | |
dc.creator | Savić, Jasmina | |
dc.creator | Bjelobaba, Ivana | |
dc.creator | Lavrnja, Irena | |
dc.creator | Vasić, Vesna | |
dc.creator | Stojiljković, Mirjana | |
dc.creator | Nedeljković, Nadežda | |
dc.date.accessioned | 2016-05-23T11:00:11Z | |
dc.date.available | 2016-05-23T11:00:11Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 1452-8266 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/2093 | |
dc.description.abstract | Background: Cortical stab injury (CSI) induces changes in the activity,
expression and cellular distribution of specific ectonucleotidases at
the injury site. Also, several experimentally induced neuropathologies
are associated with changes in soluble ectonucleotidase activities in
the plasma and serum, whilst various insults to the brain alter purine
compounds levels in cerebrospinal fluid, but also in serum, indicating
that insults to the brain may induce alterations in nucleotides release
and rate of their hydrolysis in the vascular system. Since adenine
nucleotides and adenosine regulate diverse cellular functions in the
vascular system, including vascular tone, platelet aggregation and
inflammatory responses of lymphocytes and macrophages, alterations of
ectonucleotidase activities in the vascular system may be relevant for
the clinical outcome of the primary insult.
Methods: We explored ectonucleotidase activities using specific enzyme
assays and determined adenine nucleotides concentrations by the UPLC
method in the rat serum after cortical stab injury.
Results: At 4-h post-injury, ATP and AMP hydrolysis increased by about
60\% and 40\%, respectively, while phosphodiesterase activity remained
unchanged. Also, at 4-h postinjury a marked decrease in ATP
concentration and more than 2-fold increase in AMP concentration were
recorded.
Conclusions: CSI induces rapid up-regulation of nucleotide catabolizing
soluble ectonucleotidases in rat serum, which leads to the observed
shift in serum nucleotide levels. The results obtained imply that
ectonucleotidases and adenine nucleotides participate in the
communication between the brain and the vascular system in physiological
and pathological conditions and thereby may be involved in the
development of various human neuropathologies. | en |
dc.description.sponsorship | Serbian Ministry of Education, Science and Technological Development
Project {[}41014, 172023] | |
dc.language | English | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41014/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172023/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Journal of Medical Biochemistry | |
dc.subject | ectonucleotidases | |
dc.subject | extracellular adenine nucleotides | |
dc.subject | brain injury | |
dc.subject | rat | |
dc.title | BRAIN INJURY ALTERS ECTONUCLEOTIDASE ACTIVITIES AND ADENINE NUCLEOTIDE
LEVELS IN RAT SERUM | en |
dc.type | article | |
dc.rights.license | BY-NC-ND | |
dcterms.abstract | Лаврња, Ирена Ц.; Бјелобаба, Ивана; Недељковиц, Надезда; Стојиљковиц, Мирјана; Васиц, Весна; Савиц, Јасмине; Лакета, Данијела; | |
dc.rights.holder | © Society of Medical Biochemists of Serbia | |
dc.citation.issue | 2 | |
dc.citation.volume | 34 | |
dc.identifier.doi | 10.2478/jomb-2014-0025 | |
dc.identifier.scopus | 2-s2.0-84925633777 | |
dc.identifier.wos | 000348895300008 | |
dc.citation.spage | 215 | |
dc.citation.epage | 222 | |
dc.type.version | publishedVersion | en |
dc.identifier.fulltext | https://radar.ibiss.bg.ac.rs/bitstream/id/5638/2015.pdf | |