dc.creator | Tadić, Vojin | |
dc.creator | Milošević, Snežana | |
dc.creator | Cingel, Aleksandar | |
dc.creator | Petrić, Marija | |
dc.creator | Trifunović-Momčilov, Milana | |
dc.creator | Antonić Reljin, Dragana | |
dc.creator | Jovan, Tadic | |
dc.creator | Subotić, Angelina | |
dc.date.accessioned | 2016-05-23T11:00:17Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 1895-104X | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/2122 | |
dc.description.abstract | Hairy root cultures of lettuce (Lactuca sativa L.) were obtained by
inoculation of cotyledonary leaves of in vitro lettuce seedlings (cvs.
Nansen and Ljubljanska ledenka) with Agrobacterium rhizogenes A4M70GUS.
Approximately in 96.7\% cvs. Nansen and in 91.2\% Ljubljanska ledenka
inoculated explants produced hairy root when they were incubated on
Murashige and Skoog (MS) half-strength medium without plant growth
regulators. A total of 54\% of all hairy root cultures expressed GUS
activity. Every hairy root represented an independent transformation
event. Line Ljubljanska ledenka 18 showed the highest biomass (5.5 times
the biomass of control root). A PCR analysis of the genomic DNA
confirmed the presence of marker and target genes in 15 hairy roots
examined. | en |
dc.description.sponsorship | Ministry of Education, Science and Technological Development of the
Republic of Serbia {[}TR-31019] | |
dc.language | English | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31019/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Central European Journal of Biology | |
dc.subject | Genetic Transformation | |
dc.subject | Agrobacterium Rhizogenes | |
dc.subject | Lettuce | |
dc.title | Production of hairy root cultures of lettuce (Lactuca sativa L.) | en |
dc.type | article | |
dc.rights.license | BY-NC-ND | |
dcterms.abstract | Aнтонић, Драгана; Јован, Тадиц; Марија, Петриц; Aлександар, Цингел; Војин, Тадиц; Снезана, Милосевиц; Aнгелина, Суботиц; Трифуновиц, Милана; | |
dc.rights.holder | © 2014 Versita Warsaw. | |
dc.citation.issue | 12 | |
dc.citation.volume | 9 | |
dc.identifier.doi | 10.2478/s11535-014-0351-9 | |
dc.identifier.scopus | 2-s2.0-84906320675 | |
dc.identifier.wos | 000340681200007 | |
dc.citation.spage | 1196 | |
dc.citation.epage | 1205 | |
dc.type.version | publishedVersion | en |
dc.identifier.fulltext | https://radar.ibiss.bg.ac.rs/bitstream/id/5721/bitstream_5721.pdf | |
dc.citation.rank | M23 | |