dc.creator | Đorđević, Miloš | |
dc.creator | Grdović, Nevena | |
dc.creator | Mihailović, Mirjana | |
dc.creator | Arambašić Jovanović, Jelena | |
dc.creator | Uskoković, Aleksandra | |
dc.creator | Rajić, Jovana | |
dc.creator | Đorđević, Marija | |
dc.creator | Tolić, Anja | |
dc.creator | Mišić, Danijela | |
dc.creator | Šiler, Branislav | |
dc.creator | Poznanović, Goran | |
dc.creator | Vidaković, Melita | |
dc.creator | Dinić, Svetlana | |
dc.date.accessioned | 2020-04-29T11:59:13Z | |
dc.date.available | 2020-04-29T11:59:13Z | |
dc.date.issued | 2020 | |
dc.identifier.issn | 0354-4664 | |
dc.identifier.uri | http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/5029 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/3651 | |
dc.description.abstract | Oxidative stress is one of the major mechanisms that underlies the damage of pancreatic b-cells and defects in insulin secretion in diabetes. As herbal preparations can alleviate oxidative stress through their redox-active secondary metabolites, in this study we investigated the cytoprotective effects of Centaurium erythraea extract (CEe) against H2O2- and SNP-induced oxidative/nitrosative stress in Rin-5F b-cells. The antioxidant activity of CEe and its effect on cell survival and insulin expression/secretion were evaluated. The CEe increased cell viability and ameliorated the disturbance of redox homeostasis in H2O2- and SNP-treated cells by decreasing DNA damage, lipid peroxidation and protein S-glutathionylation. The CEe restored GSH homeostasis in H2O2-treated b-cells and attenuated the SNP-induced disturbance of the GSH/GSSG ratio. The H2O2- and SNP-induced disruption of CAT, GPx, GR, MnSOD and CuZnSOD activities was adjusted by the CEe towards control values, as well as mRNA and protein levels of GPx, MnSOD and CAT. The CEe increased insulin expression/secretion particularly in H2O2-treated b-cells, which was in accordance with the more pronounced antioxidant effect of the CEe observed in H2O2-treated b-cells as compared to SNP-treated cells. These findings support the beneficial effect of the CEe in preventing or slowing down b-cell damage and dysfunction caused by oxidative/nitrosative stress during diabetes development. | en |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173020/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Archives of Biological Sciences | |
dc.subject | Oxidative stress | |
dc.subject | Nitrosative stress | |
dc.subject | β-cells | |
dc.subject | Centaurium erythraea | |
dc.subject | Antioxidant | |
dc.subject | Cytoprotective | |
dc.title | Centaurium erythraea extract reduces redox imbalance and improves insulin expression and secretion in pancreatic β-cells exposed to oxidative and nitrosative stress | en |
dc.type | article | en |
dc.rights.license | BY-NC-ND | |
dcterms.abstract | Ускоковић, Aлександра; Михаиловић, Мирјана; Ђорђевић, Милош; Грдовић, Невена; Aрамбашић-Јовановић, Јелена; Ђорђевић, Марија; Динић, Светлана; Видаковић, Мелита; Познановић, Горан; Шилер, Бранислав; Мишић, Данијела; Толић, Aња; Рајић, Јована; | |
dc.rights.holder | © 2020 by the Serbian Biological Society. | |
dc.citation.issue | 1 | |
dc.citation.volume | 72 | |
dc.identifier.doi | 10.2298/abs200127005d | |
dc.identifier.scopus | 2-s2.0-85084220729 | |
dc.identifier.wos | 000524505000013 | |
dc.citation.apa | Đorđević, M., Grdović, N., Mihailović, M., Arambašić-Jovanović, J., Uskoković, A., Rajić, J., et al. (2020). Centaurium erythraea extract reduces redox imbalance and improves insulin expression and secretion in pancreatic β-cells exposed to oxidative and nitrosative stress. Archives of Biological Sciences, 72(1), 117–128. | |
dc.citation.vancouver | Đorđević M, Grdović N, Mihailović M, Arambašić-Jovanović J, Uskoković A, Rajić J, Đorđević M, Tolić A, Mišić D, Šiler B, Poznanović G, Vidaković M, Dinić S. Centaurium erythraea extract reduces redox imbalance and improves insulin expression and secretion in pancreatic β-cells exposed to oxidative and nitrosative stress. Arch Biol Sci. 2020;72(1):117–28. | |
dc.citation.spage | 117 | |
dc.citation.epage | 128 | |
dc.type.version | publishedVersion | |
dc.identifier.fulltext | https://radar.ibiss.bg.ac.rs/bitstream/id/6132/ABS-72-1-117-128.pdf | |
dc.citation.rank | M23 | |