TY  - JOUR
AU  - Nikolić, Radomirka R
AU  - Zdravković-Korać, Snežana
AU  - Ninković, Slavica
AU  - Dragićević, Milan
AU  - Miljuš-Đukić, Jovanka D.
AU  - Banović, Bojana
AU  - Bohanec, Borut
AU  - Savić, Jelena
AU  - Banjac, Nevena
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/957
AB  - Resistance to the non-selective herbicide dl-phosphinothricin (PPT) was introduced into commercial Lotus corniculatus cv. Bokor by co-cultivation of cotyledons with Agrobacterium tumefaciensAGL1 harbouring the binary vector pDM805 which contains the bialaphos resistance gene (bar) from Streptomyces hygroscopicus encoding phosphinothricin acetyltransferase (PAT) and the uidA gene encoding -glucuronidase. The half-cotyledon explants were precultured on regeneration Murashige and Skoog's (MS) medium supplemented with 6-benzyladenine (BA) and 1-naphthaleneacetic acid (NAA) at 0.5mgL(-1) each, 3days prior to infection. Upon co-cultivation, the explants were cultured on PPT-free regeneration medium for 10days, and then subcultured on regeneration/selection media with increasing PPT concentrations (5-7mgL(-1)) for about 18weeks. Out of 480 initially co-cultivated explants, 272 regenerated shoots survived the entire PPT selection procedure. Resistant shoots were grown further, multiplied by tillering that was additionally promoted by PPT and rooted on hormone-free MS medium containing 5mgL(-1) PPT. Established shoot cultures, continuously maintained on the same medium, have preserved PPT resistance up to now (more than 2years). Transformed plants assessed in vitro and in a greenhouse were tolerant to the herbicide PPT at 300mgL(-1) equivalent to more than twofold the recommended field dosage for weed eradication. Applied PPT treatment did not affect the activities of glutamine synthetase (GS; EC 6.3.1.2) and NADH-dependent glutamate dehydrogenase (NADH-GDH; EC 1.4.1.2) in transformed plants. However, PPT did increase the mobility of glutamine synthetase isoforms GS1 and GS2 as well as the inhibition of an additional high mobility GS (hmGS) activity. In untransformed plants, PPT treatment reduced total GS activity by 4.4-fold while contrary the activity of NADH-GDH was increased by ninefold. All transformed herbicide-resistant plants were phenotypically normal and exhibited genomic stability, as were the untransformed plants analysed by flow cytometry. Under greenhouse conditions, they grew to maturity, flowered and set seeds. Stable integration and expression of the bar gene in T0 and T1 plants were confirmed by Southern and Western blot analysis, while integration of the reporter uidA gene did not occur. The bar gene was inherited in a Mendelian fashion by the progeny, as detected by PPT resistance. The production of PPT-resistant plants may have significant practical applications in weed control in fields of L. corniculatus.
T2  - Annals of Applied Biology
T1  - Fertile transgenic Lotus corniculatus resistant to the non-selective herbicide phosphinothricin
IS  - 3
VL  - 163
DO  - 10.1111/aab.12071
SP  - 247
EP  - 493
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_957
ER  - 

@article{
author = "Nikolić, Radomirka R and Zdravković-Korać, Snežana and Ninković, Slavica and Dragićević, Milan and Miljuš-Đukić, Jovanka D. and Banović, Bojana and Bohanec, Borut and Savić, Jelena and Banjac, Nevena",
year = "2013",
abstract = "Resistance to the non-selective herbicide dl-phosphinothricin (PPT) was introduced into commercial Lotus corniculatus cv. Bokor by co-cultivation of cotyledons with Agrobacterium tumefaciensAGL1 harbouring the binary vector pDM805 which contains the bialaphos resistance gene (bar) from Streptomyces hygroscopicus encoding phosphinothricin acetyltransferase (PAT) and the uidA gene encoding -glucuronidase. The half-cotyledon explants were precultured on regeneration Murashige and Skoog's (MS) medium supplemented with 6-benzyladenine (BA) and 1-naphthaleneacetic acid (NAA) at 0.5mgL(-1) each, 3days prior to infection. Upon co-cultivation, the explants were cultured on PPT-free regeneration medium for 10days, and then subcultured on regeneration/selection media with increasing PPT concentrations (5-7mgL(-1)) for about 18weeks. Out of 480 initially co-cultivated explants, 272 regenerated shoots survived the entire PPT selection procedure. Resistant shoots were grown further, multiplied by tillering that was additionally promoted by PPT and rooted on hormone-free MS medium containing 5mgL(-1) PPT. Established shoot cultures, continuously maintained on the same medium, have preserved PPT resistance up to now (more than 2years). Transformed plants assessed in vitro and in a greenhouse were tolerant to the herbicide PPT at 300mgL(-1) equivalent to more than twofold the recommended field dosage for weed eradication. Applied PPT treatment did not affect the activities of glutamine synthetase (GS; EC 6.3.1.2) and NADH-dependent glutamate dehydrogenase (NADH-GDH; EC 1.4.1.2) in transformed plants. However, PPT did increase the mobility of glutamine synthetase isoforms GS1 and GS2 as well as the inhibition of an additional high mobility GS (hmGS) activity. In untransformed plants, PPT treatment reduced total GS activity by 4.4-fold while contrary the activity of NADH-GDH was increased by ninefold. All transformed herbicide-resistant plants were phenotypically normal and exhibited genomic stability, as were the untransformed plants analysed by flow cytometry. Under greenhouse conditions, they grew to maturity, flowered and set seeds. Stable integration and expression of the bar gene in T0 and T1 plants were confirmed by Southern and Western blot analysis, while integration of the reporter uidA gene did not occur. The bar gene was inherited in a Mendelian fashion by the progeny, as detected by PPT resistance. The production of PPT-resistant plants may have significant practical applications in weed control in fields of L. corniculatus.",
journal = "Annals of Applied Biology",
title = "Fertile transgenic Lotus corniculatus resistant to the non-selective herbicide phosphinothricin",
number = "3",
volume = "163",
doi = "10.1111/aab.12071",
pages = "247-493",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_957"
}

Nikolić, R. R., Zdravković-Korać, S., Ninković, S., Dragićević, M., Miljuš-Đukić, J. D., Banović, B., Bohanec, B., Savić, J.,& Banjac, N.. (2013). Fertile transgenic Lotus corniculatus resistant to the non-selective herbicide phosphinothricin. in Annals of Applied Biology, 163(3), 247-493.
https://doi.org/10.1111/aab.12071
https://hdl.handle.net/21.15107/rcub_ibiss_957

Nikolić RR, Zdravković-Korać S, Ninković S, Dragićević M, Miljuš-Đukić JD, Banović B, Bohanec B, Savić J, Banjac N. Fertile transgenic Lotus corniculatus resistant to the non-selective herbicide phosphinothricin. in Annals of Applied Biology. 2013;163(3):247-493.
doi:10.1111/aab.12071
https://hdl.handle.net/21.15107/rcub_ibiss_957 .

Nikolić, Radomirka R, Zdravković-Korać, Snežana, Ninković, Slavica, Dragićević, Milan, Miljuš-Đukić, Jovanka D., Banović, Bojana, Bohanec, Borut, Savić, Jelena, Banjac, Nevena, "Fertile transgenic Lotus corniculatus resistant to the non-selective herbicide phosphinothricin" in Annals of Applied Biology, 163, no. 3 (2013):247-493,
https://doi.org/10.1111/aab.12071 .,
https://hdl.handle.net/21.15107/rcub_ibiss_957 .

TY  - JOUR
AU  - Dragičević, Milan B.
AU  - Platiša, Jelena
AU  - Nikolić, Radomirka R
AU  - Todorović, Slađana
AU  - Bogdanović, Milica
AU  - Banjac, Nevena
AU  - Simonović, Ana
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1052
AB  - Herbicide phosphinothricin (PPT) inhibits glutamine synthetase (GS), a key enzyme in nitrogen assimilation, thus causing ammonia accumulation, glutamine depletion and eventually plant death. However, the growth response of Lotus corniculatus L. plants immersed in solutions with a broad range of PPT concentrations is biphasic, with pronounced stimulating effect on biomass production at concentrations <= 50 mu M and growth inhibition at higher concentrations. The growth stimulation at low PPT concentrations is a result of activation of chloroplastic isoform GS2, while the growth suppression is caused by inhibition of both cytosolic GS1 and GS2 at higher PPT concentrations. Since the results are obtained in cell-free system (e. g. protein extracts), to which the principles of homeostasis are not applicable, this PPT effect is an unambiguous example of direct stimulation hormesis. A detailed molecular mechanism of concentration-dependent interaction of both PPT and a related GS inhibitor, methionine sulfoximine, with GS holoenzymes is proposed. The mechanism is in concurrence with all experimental and literature data.
T2  - Dose-Response
T1  - Herbicide Phosphinothricin Causes Direct Stimulation Hormesis
IS  - 3
VL  - 11
SP  - 299
EP  - 360
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1052
ER  - 

@article{
author = "Dragičević, Milan B. and Platiša, Jelena and Nikolić, Radomirka R and Todorović, Slađana and Bogdanović, Milica and Banjac, Nevena and Simonović, Ana",
year = "2013",
abstract = "Herbicide phosphinothricin (PPT) inhibits glutamine synthetase (GS), a key enzyme in nitrogen assimilation, thus causing ammonia accumulation, glutamine depletion and eventually plant death. However, the growth response of Lotus corniculatus L. plants immersed in solutions with a broad range of PPT concentrations is biphasic, with pronounced stimulating effect on biomass production at concentrations <= 50 mu M and growth inhibition at higher concentrations. The growth stimulation at low PPT concentrations is a result of activation of chloroplastic isoform GS2, while the growth suppression is caused by inhibition of both cytosolic GS1 and GS2 at higher PPT concentrations. Since the results are obtained in cell-free system (e. g. protein extracts), to which the principles of homeostasis are not applicable, this PPT effect is an unambiguous example of direct stimulation hormesis. A detailed molecular mechanism of concentration-dependent interaction of both PPT and a related GS inhibitor, methionine sulfoximine, with GS holoenzymes is proposed. The mechanism is in concurrence with all experimental and literature data.",
journal = "Dose-Response",
title = "Herbicide Phosphinothricin Causes Direct Stimulation Hormesis",
number = "3",
volume = "11",
pages = "299-360",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1052"
}

Dragičević, M. B., Platiša, J., Nikolić, R. R., Todorović, S., Bogdanović, M., Banjac, N.,& Simonović, A.. (2013). Herbicide Phosphinothricin Causes Direct Stimulation Hormesis. in Dose-Response, 11(3), 299-360.
https://hdl.handle.net/21.15107/rcub_ibiss_1052

Dragičević MB, Platiša J, Nikolić RR, Todorović S, Bogdanović M, Banjac N, Simonović A. Herbicide Phosphinothricin Causes Direct Stimulation Hormesis. in Dose-Response. 2013;11(3):299-360.
https://hdl.handle.net/21.15107/rcub_ibiss_1052 .

Dragičević, Milan B., Platiša, Jelena, Nikolić, Radomirka R, Todorović, Slađana, Bogdanović, Milica, Banjac, Nevena, Simonović, Ana, "Herbicide Phosphinothricin Causes Direct Stimulation Hormesis" in Dose-Response, 11, no. 3 (2013):299-360,
https://hdl.handle.net/21.15107/rcub_ibiss_1052 .

TY  - JOUR
AU  - Banjac, Nevena
AU  - Dmitrović, Slavica
AU  - Đorđević, Mirka
AU  - Zdravković-Korać, Snežana
AU  - Nikolić, Radomirka R
AU  - Raspor, Martin
AU  - Ćosić, Tatjana
AU  - Maksimović, Vuk M
AU  - Živković, Suzana
AU  - Krstić Milošević, Dijana
AU  - Stanišić, Mariana
AU  - Ninković, Slavica
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1150
AB  - We investigated Chenopodium murale transgenic hairy root in vitro culture system as a new tool for allelopathic assays. Transgenic hairy roots were induced by Agrobacterium rhizogenes A4M70GUS from roots, cotyledons, leaves, and internodes of C. murale seedlings. Roots were found to be the best target explants, providing transformation efficiency of up to 11.1%. Established hairy root clones differed in their morphology and growth potential. Molecular characterization of these clones was carried out by PCR, RT-PCR and histochemical GUS analyses. No differences in rol gene expression were observed. Liquid culture system of characterized hairy root clones was maintained for over 2 years. Six hairy root clones were selected for assaying the allelopathic effect of their growth medium against germination and seedling elongation of wheat and lettuce test plants. The inhibitory potential varied depending on the hairy root clone. Some transgenic clones showed significantly higher inhibition compared to wild-type roots. These results revealed that hairy roots as an independent system synthesize some bioactive substances with allelopathic activity and exude them into the growth medium. Concentrations of caffeic, ferulic and p-coumaric acids (0.07-2.85 mu mol/L) identified by HPLC analysis in the growth media were at least 1000 times lower than the inhibitory active concentration (5 mmol/L) of pure grade phenolic acids, suggesting that they have a limited role in the allelopathic phenomena of C murale. The presented hairy root system appears to be a suitable tool for further investigation of the potential and nature of root-mediated allelopathic interference of C. murale. (C) 2012 Elsevier GmbH. All rights reserved.
T2  - Journal of Plant Physiology
T1  - Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays
IS  - 12
VL  - 169
DO  - 10.1016/j.jplph.2012.04.009
SP  - 323
EP  - 1211
ER  - 

@article{
author = "Banjac, Nevena and Dmitrović, Slavica and Đorđević, Mirka and Zdravković-Korać, Snežana and Nikolić, Radomirka R and Raspor, Martin and Ćosić, Tatjana and Maksimović, Vuk M and Živković, Suzana and Krstić Milošević, Dijana and Stanišić, Mariana and Ninković, Slavica",
year = "2012",
abstract = "We investigated Chenopodium murale transgenic hairy root in vitro culture system as a new tool for allelopathic assays. Transgenic hairy roots were induced by Agrobacterium rhizogenes A4M70GUS from roots, cotyledons, leaves, and internodes of C. murale seedlings. Roots were found to be the best target explants, providing transformation efficiency of up to 11.1%. Established hairy root clones differed in their morphology and growth potential. Molecular characterization of these clones was carried out by PCR, RT-PCR and histochemical GUS analyses. No differences in rol gene expression were observed. Liquid culture system of characterized hairy root clones was maintained for over 2 years. Six hairy root clones were selected for assaying the allelopathic effect of their growth medium against germination and seedling elongation of wheat and lettuce test plants. The inhibitory potential varied depending on the hairy root clone. Some transgenic clones showed significantly higher inhibition compared to wild-type roots. These results revealed that hairy roots as an independent system synthesize some bioactive substances with allelopathic activity and exude them into the growth medium. Concentrations of caffeic, ferulic and p-coumaric acids (0.07-2.85 mu mol/L) identified by HPLC analysis in the growth media were at least 1000 times lower than the inhibitory active concentration (5 mmol/L) of pure grade phenolic acids, suggesting that they have a limited role in the allelopathic phenomena of C murale. The presented hairy root system appears to be a suitable tool for further investigation of the potential and nature of root-mediated allelopathic interference of C. murale. (C) 2012 Elsevier GmbH. All rights reserved.",
journal = "Journal of Plant Physiology",
title = "Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays",
number = "12",
volume = "169",
doi = "10.1016/j.jplph.2012.04.009",
pages = "323-1211"
}

Banjac, N., Dmitrović, S., Đorđević, M., Zdravković-Korać, S., Nikolić, R. R., Raspor, M., Ćosić, T., Maksimović, V. M., Živković, S., Krstić Milošević, D., Stanišić, M.,& Ninković, S.. (2012). Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays. in Journal of Plant Physiology, 169(12), 323-1211.
https://doi.org/10.1016/j.jplph.2012.04.009

Banjac N, Dmitrović S, Đorđević M, Zdravković-Korać S, Nikolić RR, Raspor M, Ćosić T, Maksimović VM, Živković S, Krstić Milošević D, Stanišić M, Ninković S. Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays. in Journal of Plant Physiology. 2012;169(12):323-1211.
doi:10.1016/j.jplph.2012.04.009 .

Banjac, Nevena, Dmitrović, Slavica, Đorđević, Mirka, Zdravković-Korać, Snežana, Nikolić, Radomirka R, Raspor, Martin, Ćosić, Tatjana, Maksimović, Vuk M, Živković, Suzana, Krstić Milošević, Dijana, Stanišić, Mariana, Ninković, Slavica, "Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays" in Journal of Plant Physiology, 169, no. 12 (2012):323-1211,
https://doi.org/10.1016/j.jplph.2012.04.009 . .

TY  - JOUR
AU  - Banjac, Nevena
AU  - Stanišić, Mariana
AU  - Milojević, Jelena
AU  - Tubić, Ljiljana B
AU  - Ćosić, Tatjana
AU  - Nikolić, Radomirka R
AU  - Ninković, Slavica
AU  - Miletić, Rade K
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1152
AB  - An efficient in vitro shoot regeneration method from leaf explants of apple cultivars Golden Delicious and Melrose by optimization of regeneration medium, explant type and orientation, dark pre-treatment, and gelling agent is presented. Murashige and Skoog's regeneration medium containing 22 mu M thidiazuron (TDZ) and 1.5 mu M indole-3-butyric acid (IBA) (M2 medium) was superior for regeneration as well as for subsequent shoot multiplication in both cultivars, providing regeneration frequency of 95% or higher in the best combination with other factors. Pre-incubation in the dark proved to be an essential factor for regeneration. The use of agar as a gelling agent provides satisfactory regeneration frequency compared with media gelled with Phyta-gel (TM). Leaf explants of cv. Melrose with adaxial surface in contact with M2 medium and those of cv. Golden Delicious orientated contrary regenerated the highest mean number of shoots per explant. Under optimal conditions, a maximal index of shoot-forming capacity of 11.44 and 6.30 for 'Melrose' and 'Golden Delicious', respectively, was achieved. Regenerated shoots were successfully rooted and acclimated ex vitro.
T2  - Hortscience
T1  - Optimization of In Vitro Regeneration from Leaf Explants of Apple Cultivars Golden Delicious and Melrose
IS  - 8
VL  - 47
DO  - 10.21273/HORTSCI.47.8.1117
SP  - 1337
EP  - 1122
ER  - 

@article{
author = "Banjac, Nevena and Stanišić, Mariana and Milojević, Jelena and Tubić, Ljiljana B and Ćosić, Tatjana and Nikolić, Radomirka R and Ninković, Slavica and Miletić, Rade K",
year = "2012",
abstract = "An efficient in vitro shoot regeneration method from leaf explants of apple cultivars Golden Delicious and Melrose by optimization of regeneration medium, explant type and orientation, dark pre-treatment, and gelling agent is presented. Murashige and Skoog's regeneration medium containing 22 mu M thidiazuron (TDZ) and 1.5 mu M indole-3-butyric acid (IBA) (M2 medium) was superior for regeneration as well as for subsequent shoot multiplication in both cultivars, providing regeneration frequency of 95% or higher in the best combination with other factors. Pre-incubation in the dark proved to be an essential factor for regeneration. The use of agar as a gelling agent provides satisfactory regeneration frequency compared with media gelled with Phyta-gel (TM). Leaf explants of cv. Melrose with adaxial surface in contact with M2 medium and those of cv. Golden Delicious orientated contrary regenerated the highest mean number of shoots per explant. Under optimal conditions, a maximal index of shoot-forming capacity of 11.44 and 6.30 for 'Melrose' and 'Golden Delicious', respectively, was achieved. Regenerated shoots were successfully rooted and acclimated ex vitro.",
journal = "Hortscience",
title = "Optimization of In Vitro Regeneration from Leaf Explants of Apple Cultivars Golden Delicious and Melrose",
number = "8",
volume = "47",
doi = "10.21273/HORTSCI.47.8.1117",
pages = "1337-1122"
}

Banjac, N., Stanišić, M., Milojević, J., Tubić, L. B., Ćosić, T., Nikolić, R. R., Ninković, S.,& Miletić, R. K.. (2012). Optimization of In Vitro Regeneration from Leaf Explants of Apple Cultivars Golden Delicious and Melrose. in Hortscience, 47(8), 1337-1122.
https://doi.org/10.21273/HORTSCI.47.8.1117

Banjac N, Stanišić M, Milojević J, Tubić LB, Ćosić T, Nikolić RR, Ninković S, Miletić RK. Optimization of In Vitro Regeneration from Leaf Explants of Apple Cultivars Golden Delicious and Melrose. in Hortscience. 2012;47(8):1337-1122.
doi:10.21273/HORTSCI.47.8.1117 .

Banjac, Nevena, Stanišić, Mariana, Milojević, Jelena, Tubić, Ljiljana B, Ćosić, Tatjana, Nikolić, Radomirka R, Ninković, Slavica, Miletić, Rade K, "Optimization of In Vitro Regeneration from Leaf Explants of Apple Cultivars Golden Delicious and Melrose" in Hortscience, 47, no. 8 (2012):1337-1122,
https://doi.org/10.21273/HORTSCI.47.8.1117 . .

TY  - JOUR
AU  - Janković, Teodora
AU  - Vinterhalter, Branka
AU  - Krstić Milošević, Dijana
AU  - Nikolić, Radomirka R
AU  - Vinterhalter, Dragan V
AU  - Milosavljević, Slobodan M
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1282
AB  - Shoot cultures of Gentianella bulgarica established from seedling epicotyls were maintained on MS medium supplemented with BA 0.2 mg l(-1) + NAA 0.1 mg l(-1). Cultures were prone to precocious flowering requiring the use of small shoot buds for multiplication purposes. The contents of three xanthone compounds identified as DGL, BGL, and DMB, in different plant material were determined by HPLC. The analysis revealed that the production of xanthones was affected by different concentrations of BA in medium. Shoot cultures grown at higher BA concentrations contained more DGL than material grown in nature. The concentrations of other two xanthones were lower in shoot cultures than in plants from nature. The radical scavenging activity of plant extracts and xanthones was investigated by DPPH test. Samples from plants grown in nature showed the highest activity (IC(50) = 0.26 mg ml(-1)), while the extracts of shoot cultures grown in media with higher concentrations of BA showed moderate activities (IC(50) from 1.6 to 4.4 mg ml(-1)).
T2  - Acta Physiologiae Plantarum
T1  - Xanthone compounds in shoot cultures of Gentianella bulgarica
IS  - 4
VL  - 33
EP  - 1520
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1282
ER  - 

@article{
author = "Janković, Teodora and Vinterhalter, Branka and Krstić Milošević, Dijana and Nikolić, Radomirka R and Vinterhalter, Dragan V and Milosavljević, Slobodan M",
year = "2011",
abstract = "Shoot cultures of Gentianella bulgarica established from seedling epicotyls were maintained on MS medium supplemented with BA 0.2 mg l(-1) + NAA 0.1 mg l(-1). Cultures were prone to precocious flowering requiring the use of small shoot buds for multiplication purposes. The contents of three xanthone compounds identified as DGL, BGL, and DMB, in different plant material were determined by HPLC. The analysis revealed that the production of xanthones was affected by different concentrations of BA in medium. Shoot cultures grown at higher BA concentrations contained more DGL than material grown in nature. The concentrations of other two xanthones were lower in shoot cultures than in plants from nature. The radical scavenging activity of plant extracts and xanthones was investigated by DPPH test. Samples from plants grown in nature showed the highest activity (IC(50) = 0.26 mg ml(-1)), while the extracts of shoot cultures grown in media with higher concentrations of BA showed moderate activities (IC(50) from 1.6 to 4.4 mg ml(-1)).",
journal = "Acta Physiologiae Plantarum",
title = "Xanthone compounds in shoot cultures of Gentianella bulgarica",
number = "4",
volume = "33",
pages = "1520",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1282"
}

Janković, T., Vinterhalter, B., Krstić Milošević, D., Nikolić, R. R., Vinterhalter, D. V.,& Milosavljević, S. M.. (2011). Xanthone compounds in shoot cultures of Gentianella bulgarica. in Acta Physiologiae Plantarum, 33(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1282

Janković T, Vinterhalter B, Krstić Milošević D, Nikolić RR, Vinterhalter DV, Milosavljević SM. Xanthone compounds in shoot cultures of Gentianella bulgarica. in Acta Physiologiae Plantarum. 2011;33(4):null-1520.
https://hdl.handle.net/21.15107/rcub_ibiss_1282 .

Janković, Teodora, Vinterhalter, Branka, Krstić Milošević, Dijana, Nikolić, Radomirka R, Vinterhalter, Dragan V, Milosavljević, Slobodan M, "Xanthone compounds in shoot cultures of Gentianella bulgarica" in Acta Physiologiae Plantarum, 33, no. 4 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1282 .

TY  - JOUR
AU  - Dodig, Dejan
AU  - Zorić, Miroslav Z
AU  - Banjac, Nevena
AU  - Nikolić, Radomirka R
AU  - King, Stephen R
AU  - Lalević, Blazo T
AU  - Surlan-Momirović, Gordana
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1416
AB  - Availability of immature embryos as explants to establish wheat (Triticum aestivum L.) by tissue culture can be limited by climatic factors and the lack of high quality embryos frequently hampers experimentation. This study evaluates the effects of rainfall, various temperature-based variables and sunshine duration on tissue culture response (TCR) traits including callus formation (CF), regenerating calli (RC), and number of plants per embryo (PPE) for 96 wheat genotypes of worldwide origin. The objectives of this study were to evaluate the significance of a particular climatic factor on TCR traits and to determine the period of wheat growth during which these factors were the most effective. The genotypes were grown in an experimental field during three seasons differing in meteorological conditions. The relationships between TCR traits and climatic factors within three time periods of wheat growth: 2, 6 and 10 weeks prior to embryo sampling were analysed by biplot analysis. The tissue culture traits were influenced at very different degrees by climatic factors: from 16.8% (RC) to 69.8% (CF). Donor plant environment with high temperatures and low rainfalls reduced (p < 0.05) the tissue culture performance of wheat genotypes. Callus formation was most sensitive to the temperature based factors. The environmental conditions between flowering and the medium milk stage were the most important for CF, while RC and PPE were not particularly related to any period.
T2  - Scientia Agricola
T1  - Morphogenetic responses of embryo culture of wheat related to environment culture conditions of the explant donor plant
IS  - 3
VL  - 67
EP  - 300
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1416
ER  - 

@article{
author = "Dodig, Dejan and Zorić, Miroslav Z and Banjac, Nevena and Nikolić, Radomirka R and King, Stephen R and Lalević, Blazo T and Surlan-Momirović, Gordana",
year = "2010",
abstract = "Availability of immature embryos as explants to establish wheat (Triticum aestivum L.) by tissue culture can be limited by climatic factors and the lack of high quality embryos frequently hampers experimentation. This study evaluates the effects of rainfall, various temperature-based variables and sunshine duration on tissue culture response (TCR) traits including callus formation (CF), regenerating calli (RC), and number of plants per embryo (PPE) for 96 wheat genotypes of worldwide origin. The objectives of this study were to evaluate the significance of a particular climatic factor on TCR traits and to determine the period of wheat growth during which these factors were the most effective. The genotypes were grown in an experimental field during three seasons differing in meteorological conditions. The relationships between TCR traits and climatic factors within three time periods of wheat growth: 2, 6 and 10 weeks prior to embryo sampling were analysed by biplot analysis. The tissue culture traits were influenced at very different degrees by climatic factors: from 16.8% (RC) to 69.8% (CF). Donor plant environment with high temperatures and low rainfalls reduced (p < 0.05) the tissue culture performance of wheat genotypes. Callus formation was most sensitive to the temperature based factors. The environmental conditions between flowering and the medium milk stage were the most important for CF, while RC and PPE were not particularly related to any period.",
journal = "Scientia Agricola",
title = "Morphogenetic responses of embryo culture of wheat related to environment culture conditions of the explant donor plant",
number = "3",
volume = "67",
pages = "300",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1416"
}

Dodig, D., Zorić, M. Z., Banjac, N., Nikolić, R. R., King, S. R., Lalević, B. T.,& Surlan-Momirović, G.. (2010). Morphogenetic responses of embryo culture of wheat related to environment culture conditions of the explant donor plant. in Scientia Agricola, 67(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1416

Dodig D, Zorić MZ, Banjac N, Nikolić RR, King SR, Lalević BT, Surlan-Momirović G. Morphogenetic responses of embryo culture of wheat related to environment culture conditions of the explant donor plant. in Scientia Agricola. 2010;67(3):null-300.
https://hdl.handle.net/21.15107/rcub_ibiss_1416 .

Dodig, Dejan, Zorić, Miroslav Z, Banjac, Nevena, Nikolić, Radomirka R, King, Stephen R, Lalević, Blazo T, Surlan-Momirović, Gordana, "Morphogenetic responses of embryo culture of wheat related to environment culture conditions of the explant donor plant" in Scientia Agricola, 67, no. 3 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1416 .

TY  - CONF
AU  - Ninković, Slavica
AU  - Smigocki, Ann
AU  - Nikolić, Radomirka R
AU  - Banjac, Nevena
AU  - Cingel, Aleksandar
AU  - Savić, Jelena M.
AU  - Đorđević, Tatjana
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1399
C3  - In Vitro Cellular & Developmental Biology-Animal
T1  - Genetic Engineering of Lotus corniculatus L Forage Legume with Proteinase Inhibitor Genes
IS  - null
VL  - 46
EP  - S191
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1399
ER  - 

@conference{
author = "Ninković, Slavica and Smigocki, Ann and Nikolić, Radomirka R and Banjac, Nevena and Cingel, Aleksandar and Savić, Jelena M. and Đorđević, Tatjana",
year = "2010",
journal = "In Vitro Cellular & Developmental Biology-Animal",
title = "Genetic Engineering of Lotus corniculatus L Forage Legume with Proteinase Inhibitor Genes",
number = "null",
volume = "46",
pages = "S191",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1399"
}

Ninković, S., Smigocki, A., Nikolić, R. R., Banjac, N., Cingel, A., Savić, J. M.,& Đorđević, T.. (2010). Genetic Engineering of Lotus corniculatus L Forage Legume with Proteinase Inhibitor Genes. in In Vitro Cellular & Developmental Biology-Animal, 46(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1399

Ninković S, Smigocki A, Nikolić RR, Banjac N, Cingel A, Savić JM, Đorđević T. Genetic Engineering of Lotus corniculatus L Forage Legume with Proteinase Inhibitor Genes. in In Vitro Cellular & Developmental Biology-Animal. 2010;46(null):null-S191.
https://hdl.handle.net/21.15107/rcub_ibiss_1399 .

Ninković, Slavica, Smigocki, Ann, Nikolić, Radomirka R, Banjac, Nevena, Cingel, Aleksandar, Savić, Jelena M., Đorđević, Tatjana, "Genetic Engineering of Lotus corniculatus L Forage Legume with Proteinase Inhibitor Genes" in In Vitro Cellular & Developmental Biology-Animal, 46, no. null (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1399 .

TY  - JOUR
AU  - Nikolić, Radomirka R
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Vinterhalter, Branka
AU  - Zdravković-Korać, Snežana
AU  - Nešković, Mirjana
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1337
AB  - Shoots of Lotus corniculatus L., previously transformed with Agrobacterium tumefaciens LBA4404/pTOK233, were grown in gibberellic acid (GA(3))-containing media in an attempt to improve their growth and multiplication. Nodal stem segments of four poorly multiplying clones, a clone with very good multiplication, and two non-transformed clones were incubated in media containing GA(3) for 3 weeks, and then returned to a hormone-free medium for a further 3-5 subculture periods. Gibberellic acid increased the number of axillary buds from one in controls to 2-13 shoots during the first 3 weeks of subculture in GA(3)-containing media. The multiplied buds, which apparently derived from additional axillaries, continued to multiply in hormone-free medium, producing bunches of more than 50 shoots after 9 weeks. In addition, many nodal segments also produced callus tissues at the basal end, or along the internode below the node, in which abundant shoot meristems formed de novo. Histological examination confirmed their origin via organogenesis. About 25% of regenerated shoots rooted spontaneously. The application of auxins significantly improved rooting in more than 75% of all clones. Well-developed plantlets were produced upon the transfer of rooted shoots to pots. No differences in responses to hormones were observed between LBA4404/pTOK233-transformed clones and non-transformed clones, indicating that the reaction to GA(3) was not the consequence of transformation events. The results point to a possible involvement of GA(3) in lateral branching. They might also be recommended as a procedure suitable for increasing the production of transformed plants in L. corniculatus.
T2  - Plant Growth Regulation
T1  - Gibberellic acid promotes in vitro regeneration and shoot multiplication in Lotus corniculatus L.
IS  - 2
VL  - 62
EP  - 188
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1337
ER  - 

@article{
author = "Nikolić, Radomirka R and Banjac, Nevena and Ninković, Slavica and Vinterhalter, Branka and Zdravković-Korać, Snežana and Nešković, Mirjana",
year = "2010",
abstract = "Shoots of Lotus corniculatus L., previously transformed with Agrobacterium tumefaciens LBA4404/pTOK233, were grown in gibberellic acid (GA(3))-containing media in an attempt to improve their growth and multiplication. Nodal stem segments of four poorly multiplying clones, a clone with very good multiplication, and two non-transformed clones were incubated in media containing GA(3) for 3 weeks, and then returned to a hormone-free medium for a further 3-5 subculture periods. Gibberellic acid increased the number of axillary buds from one in controls to 2-13 shoots during the first 3 weeks of subculture in GA(3)-containing media. The multiplied buds, which apparently derived from additional axillaries, continued to multiply in hormone-free medium, producing bunches of more than 50 shoots after 9 weeks. In addition, many nodal segments also produced callus tissues at the basal end, or along the internode below the node, in which abundant shoot meristems formed de novo. Histological examination confirmed their origin via organogenesis. About 25% of regenerated shoots rooted spontaneously. The application of auxins significantly improved rooting in more than 75% of all clones. Well-developed plantlets were produced upon the transfer of rooted shoots to pots. No differences in responses to hormones were observed between LBA4404/pTOK233-transformed clones and non-transformed clones, indicating that the reaction to GA(3) was not the consequence of transformation events. The results point to a possible involvement of GA(3) in lateral branching. They might also be recommended as a procedure suitable for increasing the production of transformed plants in L. corniculatus.",
journal = "Plant Growth Regulation",
title = "Gibberellic acid promotes in vitro regeneration and shoot multiplication in Lotus corniculatus L.",
number = "2",
volume = "62",
pages = "188",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1337"
}

Nikolić, R. R., Banjac, N., Ninković, S., Vinterhalter, B., Zdravković-Korać, S.,& Nešković, M.. (2010). Gibberellic acid promotes in vitro regeneration and shoot multiplication in Lotus corniculatus L.. in Plant Growth Regulation, 62(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1337

Nikolić RR, Banjac N, Ninković S, Vinterhalter B, Zdravković-Korać S, Nešković M. Gibberellic acid promotes in vitro regeneration and shoot multiplication in Lotus corniculatus L.. in Plant Growth Regulation. 2010;62(2):null-188.
https://hdl.handle.net/21.15107/rcub_ibiss_1337 .

Nikolić, Radomirka R, Banjac, Nevena, Ninković, Slavica, Vinterhalter, Branka, Zdravković-Korać, Snežana, Nešković, Mirjana, "Gibberellic acid promotes in vitro regeneration and shoot multiplication in Lotus corniculatus L." in Plant Growth Regulation, 62, no. 2 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1337 .

TY  - JOUR
AU  - Banjac, Nevena
AU  - Dodig, Dejan
AU  - Nikolić, Radomirka R
AU  - Ninković, Slavica
AU  - Vinterhalter, Dragan V
AU  - Vinterhalter, Branka
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1445
AB  - The influence of donor plant growth conditions in years 2003, 2004, and 2005 on tissue culture response (TCR) traits of 96 wheat genotypes was evaluated. Immature embryos, collected 12-15 days after anthesis from field-grown plants, were cultured on Murashige and Skoog medium containing 2 mg/l 2,4-D followed by their transfer to a growth regulator-free medium. Donor plants growth in a season 2003 characterized by drought and heat stress, as compared to favorable growth seasons 2004 and 2005, resulted in a increased variability and a decreased percentage of callus formation and the number of regenerating calli and plants regenerated per embryo in all genotypes, except genotype Florida that exhibited a significantly increased number of regenerating calli in 2003. The reduction rate of regenerating callus formation depended on genotype, and it was the highest in Magnif 41, NS66/92, and Mexico 3. The results suggest that the impact of donor plant growth conditions on TCR traits can be as large as the effect of the genotype, which should be taken into account when planning transformation work with wheat.
T2  - Russian Journal of Plant Physiology
T1  - Effects of donor plant environmental conditions on immature embryo cultures derived from worldwide origin wheat genotypes
IS  - 4
VL  - 56
EP  - 545
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1445
ER  - 

@article{
author = "Banjac, Nevena and Dodig, Dejan and Nikolić, Radomirka R and Ninković, Slavica and Vinterhalter, Dragan V and Vinterhalter, Branka",
year = "2009",
abstract = "The influence of donor plant growth conditions in years 2003, 2004, and 2005 on tissue culture response (TCR) traits of 96 wheat genotypes was evaluated. Immature embryos, collected 12-15 days after anthesis from field-grown plants, were cultured on Murashige and Skoog medium containing 2 mg/l 2,4-D followed by their transfer to a growth regulator-free medium. Donor plants growth in a season 2003 characterized by drought and heat stress, as compared to favorable growth seasons 2004 and 2005, resulted in a increased variability and a decreased percentage of callus formation and the number of regenerating calli and plants regenerated per embryo in all genotypes, except genotype Florida that exhibited a significantly increased number of regenerating calli in 2003. The reduction rate of regenerating callus formation depended on genotype, and it was the highest in Magnif 41, NS66/92, and Mexico 3. The results suggest that the impact of donor plant growth conditions on TCR traits can be as large as the effect of the genotype, which should be taken into account when planning transformation work with wheat.",
journal = "Russian Journal of Plant Physiology",
title = "Effects of donor plant environmental conditions on immature embryo cultures derived from worldwide origin wheat genotypes",
number = "4",
volume = "56",
pages = "545",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1445"
}

Banjac, N., Dodig, D., Nikolić, R. R., Ninković, S., Vinterhalter, D. V.,& Vinterhalter, B.. (2009). Effects of donor plant environmental conditions on immature embryo cultures derived from worldwide origin wheat genotypes. in Russian Journal of Plant Physiology, 56(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1445

Banjac N, Dodig D, Nikolić RR, Ninković S, Vinterhalter DV, Vinterhalter B. Effects of donor plant environmental conditions on immature embryo cultures derived from worldwide origin wheat genotypes. in Russian Journal of Plant Physiology. 2009;56(4):null-545.
https://hdl.handle.net/21.15107/rcub_ibiss_1445 .

Banjac, Nevena, Dodig, Dejan, Nikolić, Radomirka R, Ninković, Slavica, Vinterhalter, Dragan V, Vinterhalter, Branka, "Effects of donor plant environmental conditions on immature embryo cultures derived from worldwide origin wheat genotypes" in Russian Journal of Plant Physiology, 56, no. 4 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1445 .

TY  - JOUR
AU  - Dodig, Dejan
AU  - Zorić, Miroslav Z
AU  - Banjac, Nevena
AU  - Nikolić, Radomirka R
AU  - Surlan-Momirović, Gordana
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1513
AB  - There are only a few reports about the relationship between tissue culture and agronomic traits in wheat. In an attempt to enlarge the knowledge in this area, 96 wheat genotypes of worldwide origin were evaluated for their tissue culture response as well as the agronomic performance in the three-year field trials. Plant regeneration was achieved from immature embryos collected 12-15 days after anthesis. Associations between tissue culture and agronomic traits were surveyed by the correlation and path coefficient analyses. A trait-association by year biplot was applied to understand environmental effects on these relationships. All significant trait-associations were of a moderate magnitude and were responsive to the environment. The path coefficient analysis revealed that a grain yield had the highest positive direct effect on a callus formation while the kernel number per spike had the highest positive direct effect on regenerative calli and plant number per embryo. Nevertheless, only productive tillering had significant (positive) direct effect on all studied tissue culture traits.
T2  - Plant Cell Tissue and Organ Culture
T1  - Tissue culture and agronomic traits relationship in wheat
IS  - 1
VL  - 95
EP  - 114
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1513
ER  - 

@article{
author = "Dodig, Dejan and Zorić, Miroslav Z and Banjac, Nevena and Nikolić, Radomirka R and Surlan-Momirović, Gordana",
year = "2008",
abstract = "There are only a few reports about the relationship between tissue culture and agronomic traits in wheat. In an attempt to enlarge the knowledge in this area, 96 wheat genotypes of worldwide origin were evaluated for their tissue culture response as well as the agronomic performance in the three-year field trials. Plant regeneration was achieved from immature embryos collected 12-15 days after anthesis. Associations between tissue culture and agronomic traits were surveyed by the correlation and path coefficient analyses. A trait-association by year biplot was applied to understand environmental effects on these relationships. All significant trait-associations were of a moderate magnitude and were responsive to the environment. The path coefficient analysis revealed that a grain yield had the highest positive direct effect on a callus formation while the kernel number per spike had the highest positive direct effect on regenerative calli and plant number per embryo. Nevertheless, only productive tillering had significant (positive) direct effect on all studied tissue culture traits.",
journal = "Plant Cell Tissue and Organ Culture",
title = "Tissue culture and agronomic traits relationship in wheat",
number = "1",
volume = "95",
pages = "114",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1513"
}

Dodig, D., Zorić, M. Z., Banjac, N., Nikolić, R. R.,& Surlan-Momirović, G.. (2008). Tissue culture and agronomic traits relationship in wheat. in Plant Cell Tissue and Organ Culture, 95(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1513

Dodig D, Zorić MZ, Banjac N, Nikolić RR, Surlan-Momirović G. Tissue culture and agronomic traits relationship in wheat. in Plant Cell Tissue and Organ Culture. 2008;95(1):null-114.
https://hdl.handle.net/21.15107/rcub_ibiss_1513 .

Dodig, Dejan, Zorić, Miroslav Z, Banjac, Nevena, Nikolić, Radomirka R, Surlan-Momirović, Gordana, "Tissue culture and agronomic traits relationship in wheat" in Plant Cell Tissue and Organ Culture, 95, no. 1 (2008),
https://hdl.handle.net/21.15107/rcub_ibiss_1513 .

TY  - JOUR
AU  - Banjac, Nevena
AU  - Nikolić, Radomirka R
AU  - Ninković, Slavica
AU  - Miljuš-Đukić, Jovanka D.
AU  - Nešković, Mirjana
PY  - 2004
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1747
AB  - The use of two Agrobacterium tumefaciens strains for transformation of Triticum aestivum L. cv. Vesna was studied. Immature embryos, isolated 15 d after pollination, were co-cultivated with the super-binary LBA4404/pTOK233 and the binary AGL1/pDM805 vectors. While the transient GUS-intron expression was high (69.9 and 80.0%), the number of plants regenerated on selective media containing hygromycin or phosphinotricin did not exceed 0.4 and 0.13%, respectively. Nevertheless, the regenerated plants were fertile and produced seeds. The T-0 plants, as well as the T-1 seedlings, displayed the activity in the beta-glucuronidase histochemical assay and a positive signal in PCR analysis for the presence of uidA gene sequences in their genomes. The data suggest that the transformation of wheat cv. Vesna with both Agrobacterium strains is feasible.
T2  - Biologia Plantarum
T1  - Agrobacterium-mediated transformation and plant regeneration of Triticum aestivum L.
IS  - 2
VL  - 48
EP  - 184
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1747
ER  - 

@article{
author = "Banjac, Nevena and Nikolić, Radomirka R and Ninković, Slavica and Miljuš-Đukić, Jovanka D. and Nešković, Mirjana",
year = "2004",
abstract = "The use of two Agrobacterium tumefaciens strains for transformation of Triticum aestivum L. cv. Vesna was studied. Immature embryos, isolated 15 d after pollination, were co-cultivated with the super-binary LBA4404/pTOK233 and the binary AGL1/pDM805 vectors. While the transient GUS-intron expression was high (69.9 and 80.0%), the number of plants regenerated on selective media containing hygromycin or phosphinotricin did not exceed 0.4 and 0.13%, respectively. Nevertheless, the regenerated plants were fertile and produced seeds. The T-0 plants, as well as the T-1 seedlings, displayed the activity in the beta-glucuronidase histochemical assay and a positive signal in PCR analysis for the presence of uidA gene sequences in their genomes. The data suggest that the transformation of wheat cv. Vesna with both Agrobacterium strains is feasible.",
journal = "Biologia Plantarum",
title = "Agrobacterium-mediated transformation and plant regeneration of Triticum aestivum L.",
number = "2",
volume = "48",
pages = "184",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1747"
}

Banjac, N., Nikolić, R. R., Ninković, S., Miljuš-Đukić, J. D.,& Nešković, M.. (2004). Agrobacterium-mediated transformation and plant regeneration of Triticum aestivum L.. in Biologia Plantarum, 48(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1747

Banjac N, Nikolić RR, Ninković S, Miljuš-Đukić JD, Nešković M. Agrobacterium-mediated transformation and plant regeneration of Triticum aestivum L.. in Biologia Plantarum. 2004;48(2):null-184.
https://hdl.handle.net/21.15107/rcub_ibiss_1747 .

Banjac, Nevena, Nikolić, Radomirka R, Ninković, Slavica, Miljuš-Đukić, Jovanka D., Nešković, Mirjana, "Agrobacterium-mediated transformation and plant regeneration of Triticum aestivum L." in Biologia Plantarum, 48, no. 2 (2004),
https://hdl.handle.net/21.15107/rcub_ibiss_1747 .