TY  - JOUR
AU  - Đorđević, Sofija
AU  - Nedić, Nebojša
AU  - Pavlović, Aleksandra
AU  - Milojković-Opsenica, Dušanka
AU  - Tešić, Živoslav
AU  - Gašić, Uroš
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S2210803322000495
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5012
AB  - Introduction The aim of this study was to find the best conditions for the extraction of rutin and quercetin from Sophora flower in acacia honey as an extractant, and to formulate a new product – honey with added value. Methods For determination of the optimal extraction conditions of the above mentioned compounds, 10% m/m of the dried Sophora flower was added to honey and the concentration of rutin and quercetin was monitored, at 4 °C and room temperature at 1, 15, 40 and 90 day intervals. The analyses of rutin and quercetin content in honey were performed by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. The content of rutin and quercetin in the flower was also determined to check the quality of the herbal drug used, as well as to evaluate the effectiveness of the extraction. Results The extraction of rutin and quercetin, in a honey sample using 10% of the Sophora flower, stored at room temperature, for 40 days, was the most intense one (150.24 mg/kg of rutin and 1338.93 mg/kg of quercetin). Sophora flower corresponds to the pharmacopoeia requirements for the quality of an herbal drug. The concentrations of rutin and quercetin in acacia honey were 0.08 and 0.14 mg/kg, respectively. The concentration of rutin in honey from the melliferous pasture of the Sophora flower was 0.40 mg/kg, while quercetin was below the detection limit (0.1 mg/kg). Conclusions Honey, enriched with rutin and quercetin from Sophora flower, had sensory acceptability and pleasant taste and aroma.
T2  - Journal of Herbal Medicine
T1  - Honey with added value – enriched with rutin and quercetin from Sophora flower
VL  - 34
DO  - 10.1016/j.hermed.2022.100580
SP  - 100580
ER  - 

@article{
author = "Đorđević, Sofija and Nedić, Nebojša and Pavlović, Aleksandra and Milojković-Opsenica, Dušanka and Tešić, Živoslav and Gašić, Uroš",
year = "2022",
abstract = "Introduction The aim of this study was to find the best conditions for the extraction of rutin and quercetin from Sophora flower in acacia honey as an extractant, and to formulate a new product – honey with added value. Methods For determination of the optimal extraction conditions of the above mentioned compounds, 10% m/m of the dried Sophora flower was added to honey and the concentration of rutin and quercetin was monitored, at 4 °C and room temperature at 1, 15, 40 and 90 day intervals. The analyses of rutin and quercetin content in honey were performed by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. The content of rutin and quercetin in the flower was also determined to check the quality of the herbal drug used, as well as to evaluate the effectiveness of the extraction. Results The extraction of rutin and quercetin, in a honey sample using 10% of the Sophora flower, stored at room temperature, for 40 days, was the most intense one (150.24 mg/kg of rutin and 1338.93 mg/kg of quercetin). Sophora flower corresponds to the pharmacopoeia requirements for the quality of an herbal drug. The concentrations of rutin and quercetin in acacia honey were 0.08 and 0.14 mg/kg, respectively. The concentration of rutin in honey from the melliferous pasture of the Sophora flower was 0.40 mg/kg, while quercetin was below the detection limit (0.1 mg/kg). Conclusions Honey, enriched with rutin and quercetin from Sophora flower, had sensory acceptability and pleasant taste and aroma.",
journal = "Journal of Herbal Medicine",
title = "Honey with added value – enriched with rutin and quercetin from Sophora flower",
volume = "34",
doi = "10.1016/j.hermed.2022.100580",
pages = "100580"
}

Đorđević, S., Nedić, N., Pavlović, A., Milojković-Opsenica, D., Tešić, Ž.,& Gašić, U.. (2022). Honey with added value – enriched with rutin and quercetin from Sophora flower. in Journal of Herbal Medicine, 34, 100580.
https://doi.org/10.1016/j.hermed.2022.100580

Đorđević S, Nedić N, Pavlović A, Milojković-Opsenica D, Tešić Ž, Gašić U. Honey with added value – enriched with rutin and quercetin from Sophora flower. in Journal of Herbal Medicine. 2022;34:100580.
doi:10.1016/j.hermed.2022.100580 .

Đorđević, Sofija, Nedić, Nebojša, Pavlović, Aleksandra, Milojković-Opsenica, Dušanka, Tešić, Živoslav, Gašić, Uroš, "Honey with added value – enriched with rutin and quercetin from Sophora flower" in Journal of Herbal Medicine, 34 (2022):100580,
https://doi.org/10.1016/j.hermed.2022.100580 . .

TY  - JOUR
AU  - Ljoljić Bilić, Vanja
AU  - Gašić, Uroš
AU  - Milojković-Opsenica, Dušanka
AU  - Rimac, Hrvoje
AU  - Vuković Rodriguez, Jadranka
AU  - Vlainić, Josipa
AU  - Brlek-Gorski, Diana
AU  - Kosalec, Ivan
PY  - 2022
UR  - https://www.mdpi.com/2079-6382/11/4/492
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4960
AB  - Followed by a buildup of its phytochemical profile, Erodium cicutarium is being subjected to antimicrobial investigation guided with its ethnobotanical use. The results of performed in vitro screening on Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans strains, show that E. cicutarium has antimicrobial activity, with a particular emphasis on clinical S. aureus strains—both the methicillin sensitive (MSSA) and the methicillin resistant (MRSA) S. aureus. Experimental design consisted of general methods (the serial microdilution broth assay and the agar well diffusion assay), as well as observing bactericidal/bacteriostatic activity through time (the “time-kill” assay), investigating the effect on cell wall integrity and biofilm formation, and modulation of bacterial hemolysis. Observed antibacterial activity from above-described methods led to further activity-guided fractionation of water and methanol extracts using bioautography coupled with UHPLC-LTQ OrbiTrap MS4. It was determined that active fractions are predominantly formed by gallic acid derivatives and flavonol glycosides. Among the most active phytochemicals, galloyl-shikimic acid was identified as the most abundant compound. These results point to a direct connection between galloyl-shikimic acid and the observed E. cicutarium antibacterial activity, and open several new research approaches for future investigation.
PB  - Basel: MDPI
T2  - Antibiotics
T1  - Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus
IS  - 4
VL  - 11
DO  - 10.3390/antibiotics11040492
SP  - 492
ER  - 

@article{
author = "Ljoljić Bilić, Vanja and Gašić, Uroš and Milojković-Opsenica, Dušanka and Rimac, Hrvoje and Vuković Rodriguez, Jadranka and Vlainić, Josipa and Brlek-Gorski, Diana and Kosalec, Ivan",
year = "2022",
abstract = "Followed by a buildup of its phytochemical profile, Erodium cicutarium is being subjected to antimicrobial investigation guided with its ethnobotanical use. The results of performed in vitro screening on Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans strains, show that E. cicutarium has antimicrobial activity, with a particular emphasis on clinical S. aureus strains—both the methicillin sensitive (MSSA) and the methicillin resistant (MRSA) S. aureus. Experimental design consisted of general methods (the serial microdilution broth assay and the agar well diffusion assay), as well as observing bactericidal/bacteriostatic activity through time (the “time-kill” assay), investigating the effect on cell wall integrity and biofilm formation, and modulation of bacterial hemolysis. Observed antibacterial activity from above-described methods led to further activity-guided fractionation of water and methanol extracts using bioautography coupled with UHPLC-LTQ OrbiTrap MS4. It was determined that active fractions are predominantly formed by gallic acid derivatives and flavonol glycosides. Among the most active phytochemicals, galloyl-shikimic acid was identified as the most abundant compound. These results point to a direct connection between galloyl-shikimic acid and the observed E. cicutarium antibacterial activity, and open several new research approaches for future investigation.",
publisher = "Basel: MDPI",
journal = "Antibiotics",
title = "Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus",
number = "4",
volume = "11",
doi = "10.3390/antibiotics11040492",
pages = "492"
}

Ljoljić Bilić, V., Gašić, U., Milojković-Opsenica, D., Rimac, H., Vuković Rodriguez, J., Vlainić, J., Brlek-Gorski, D.,& Kosalec, I.. (2022). Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus. in Antibiotics
Basel: MDPI., 11(4), 492.
https://doi.org/10.3390/antibiotics11040492

Ljoljić Bilić V, Gašić U, Milojković-Opsenica D, Rimac H, Vuković Rodriguez J, Vlainić J, Brlek-Gorski D, Kosalec I. Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus. in Antibiotics. 2022;11(4):492.
doi:10.3390/antibiotics11040492 .

Ljoljić Bilić, Vanja, Gašić, Uroš, Milojković-Opsenica, Dušanka, Rimac, Hrvoje, Vuković Rodriguez, Jadranka, Vlainić, Josipa, Brlek-Gorski, Diana, Kosalec, Ivan, "Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus" in Antibiotics, 11, no. 4 (2022):492,
https://doi.org/10.3390/antibiotics11040492 . .

TY  - JOUR
AU  - Jurič, Andreja
AU  - Brčić Karačonji, Irena
AU  - Žunec, Suzana
AU  - Katić, Anja
AU  - Gašić, Uroš
AU  - Milojković Opsenica, Dušanka
AU  - Kopjar, Nevenka
PY  - 2022
UR  - https://www.tandfonline.com/doi/full/10.1080/00218839.2022.2047421
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4949
AB  - Strawberry tree (Arbutus unedo L.) honey (STH) is widely used as part of traditional medicine in the Mediterranean area, especially in the treatment of wounds, burns and infections. In this study, we investigated the cyto-/genoprotective properties of STH at cell level following short-term exposure to UVB radiation at 2 kJ/m2. Isolated human peripheral blood lymphocytes (PBL) were selected as model cells. Phenolics in STH were determined using an ultra-high-performance liquid chromatograph (UHPLC) coupled to a linear ion trap-Orbitrap hybrid mass spectrometer (LTQ Orbitrap MS), while homogentisic acid (HGA) was quantified using a gas chromatograph-mass spectrometer (GC-MS). Primary DNA damage in PBLs treated with STH before and after irradiation was assessed by alkaline comet assay, while their viability was determined using the apoptosis/necrosis assay. The levels of thiobarbituric acid reactive substances (TBARS) and glutathione (GSH) were determined in plasma samples. STH applied as pre-treatment at a concentration equivalent to its average daily portion efficiently counteracted the cytotoxic and genotoxic effects and diminished UVB-induced oxidative stress. Post-radiation treatment with STH was not as beneficial. Protective effects could be associated to the complex phytochemical profile of STH and the high content of HGA (306.8 mg/kg). It was confirmed that an intake of a complex mixture of bioactive constituents as STH prior to potential cyto/genotoxic insults stimulated a broad-spectrum of protective mechanisms, including intracellular antioxidative defence and DNA repair systems that helped pre-treated cells to counteract harmful exposures more efficiently.
PB  - Taylor and Francis Ltd.
T2  - Journal of Apicultural Research
T1  - Protective role of strawberry tree (Arbutus unedo L.) honey against cyto/genotoxic effects induced by ultraviolet B radiation in vitro
DO  - 10.1080/00218839.2022.2047421
SP  - 1
EP  - 10
ER  - 

@article{
author = "Jurič, Andreja and Brčić Karačonji, Irena and Žunec, Suzana and Katić, Anja and Gašić, Uroš and Milojković Opsenica, Dušanka and Kopjar, Nevenka",
year = "2022",
abstract = "Strawberry tree (Arbutus unedo L.) honey (STH) is widely used as part of traditional medicine in the Mediterranean area, especially in the treatment of wounds, burns and infections. In this study, we investigated the cyto-/genoprotective properties of STH at cell level following short-term exposure to UVB radiation at 2 kJ/m2. Isolated human peripheral blood lymphocytes (PBL) were selected as model cells. Phenolics in STH were determined using an ultra-high-performance liquid chromatograph (UHPLC) coupled to a linear ion trap-Orbitrap hybrid mass spectrometer (LTQ Orbitrap MS), while homogentisic acid (HGA) was quantified using a gas chromatograph-mass spectrometer (GC-MS). Primary DNA damage in PBLs treated with STH before and after irradiation was assessed by alkaline comet assay, while their viability was determined using the apoptosis/necrosis assay. The levels of thiobarbituric acid reactive substances (TBARS) and glutathione (GSH) were determined in plasma samples. STH applied as pre-treatment at a concentration equivalent to its average daily portion efficiently counteracted the cytotoxic and genotoxic effects and diminished UVB-induced oxidative stress. Post-radiation treatment with STH was not as beneficial. Protective effects could be associated to the complex phytochemical profile of STH and the high content of HGA (306.8 mg/kg). It was confirmed that an intake of a complex mixture of bioactive constituents as STH prior to potential cyto/genotoxic insults stimulated a broad-spectrum of protective mechanisms, including intracellular antioxidative defence and DNA repair systems that helped pre-treated cells to counteract harmful exposures more efficiently.",
publisher = "Taylor and Francis Ltd.",
journal = "Journal of Apicultural Research",
title = "Protective role of strawberry tree (Arbutus unedo L.) honey against cyto/genotoxic effects induced by ultraviolet B radiation in vitro",
doi = "10.1080/00218839.2022.2047421",
pages = "1-10"
}

Jurič, A., Brčić Karačonji, I., Žunec, S., Katić, A., Gašić, U., Milojković Opsenica, D.,& Kopjar, N.. (2022). Protective role of strawberry tree (Arbutus unedo L.) honey against cyto/genotoxic effects induced by ultraviolet B radiation in vitro. in Journal of Apicultural Research
Taylor and Francis Ltd.., 1-10.
https://doi.org/10.1080/00218839.2022.2047421

Jurič A, Brčić Karačonji I, Žunec S, Katić A, Gašić U, Milojković Opsenica D, Kopjar N. Protective role of strawberry tree (Arbutus unedo L.) honey against cyto/genotoxic effects induced by ultraviolet B radiation in vitro. in Journal of Apicultural Research. 2022;:1-10.
doi:10.1080/00218839.2022.2047421 .

Jurič, Andreja, Brčić Karačonji, Irena, Žunec, Suzana, Katić, Anja, Gašić, Uroš, Milojković Opsenica, Dušanka, Kopjar, Nevenka, "Protective role of strawberry tree (Arbutus unedo L.) honey against cyto/genotoxic effects induced by ultraviolet B radiation in vitro" in Journal of Apicultural Research (2022):1-10,
https://doi.org/10.1080/00218839.2022.2047421 . .

TY  - JOUR
AU  - Brčić Karačonji, Irena
AU  - Jurica, Karlo
AU  - Gašić, Uroš
AU  - Dramićanin, Aleksandra
AU  - Tešić, Živoslav
AU  - Milojković Opsenica, Dušanka
PY  - 2022
UR  - https://www.mdpi.com/2223-7747/11/1/25
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8747429
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4758
AB  - The strawberry tree (Arbutus unedo L., Ericaceae family) is an evergreen Mediterranean shrub whose leaves and fruits are used in traditional medicine due to their antioxidant, antimicrobial, antidiabetic, diuretic, and antiproliferative properties. The health benefits are mainly attributed to the presence of phenolic compounds. The aim of this study was to compare the phenolic profiles, total phenolic content (TPC), and radical scavenging activity (RSA) of A. unedo leaves and fruits collected at two locations in Croatia. Phenolic profiles were identified using an ultra-high-performance liquid chromatograph (UHPLC) coupled with a hybrid mass spectrometer (LTQ Orbitrap MS). TPC was determined by Folin-Ciocalteu's assay, while RSA was investigated using DPPH reagent. A total of 64 phenolics (60 and 42 compounds in leaves and fruits, respectively) were identified. Hyperoside and flavan-3-ols were predominant compounds in leaves, while gallocatechin and catechin were the major compounds found in fruits. To the authors' knowledge, 16 and 5 phenolics in leaves and fruits, respectively, were reported for the first time. Principal component analysis (PCA) showed that UHPLC-LTQ Orbitrap MS could be used to identify which phenolics were able to discriminate samples regarding plant tissue and geographical origin. TPC in leaves and fruits were in the ranges of 67.07-104.74 and 16.78-25.86 mg gallic acid equivalents (GAE)/g dried weight (dw), respectively. RSA for leaves and fruits were in the ranges of 408.92-430.98 and 74.30-104.04 μmol Trolox equivalents (TE)/g dw, respectively. The number of identified phenolics was lower in fruits compared to leaves. Such a large number of bioactive phenolics identified and the strong antioxidant activity pointed to A. unedo as a promising health-promoting plant and natural food preservative.
PB  - Basel: MDPI
T2  - Plants (Basel, Switzerland)
T1  - Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits.
IS  - 1
VL  - 11
DO  - 10.3390/plants11010025
SP  - 25
ER  - 

@article{
author = "Brčić Karačonji, Irena and Jurica, Karlo and Gašić, Uroš and Dramićanin, Aleksandra and Tešić, Živoslav and Milojković Opsenica, Dušanka",
year = "2022",
abstract = "The strawberry tree (Arbutus unedo L., Ericaceae family) is an evergreen Mediterranean shrub whose leaves and fruits are used in traditional medicine due to their antioxidant, antimicrobial, antidiabetic, diuretic, and antiproliferative properties. The health benefits are mainly attributed to the presence of phenolic compounds. The aim of this study was to compare the phenolic profiles, total phenolic content (TPC), and radical scavenging activity (RSA) of A. unedo leaves and fruits collected at two locations in Croatia. Phenolic profiles were identified using an ultra-high-performance liquid chromatograph (UHPLC) coupled with a hybrid mass spectrometer (LTQ Orbitrap MS). TPC was determined by Folin-Ciocalteu's assay, while RSA was investigated using DPPH reagent. A total of 64 phenolics (60 and 42 compounds in leaves and fruits, respectively) were identified. Hyperoside and flavan-3-ols were predominant compounds in leaves, while gallocatechin and catechin were the major compounds found in fruits. To the authors' knowledge, 16 and 5 phenolics in leaves and fruits, respectively, were reported for the first time. Principal component analysis (PCA) showed that UHPLC-LTQ Orbitrap MS could be used to identify which phenolics were able to discriminate samples regarding plant tissue and geographical origin. TPC in leaves and fruits were in the ranges of 67.07-104.74 and 16.78-25.86 mg gallic acid equivalents (GAE)/g dried weight (dw), respectively. RSA for leaves and fruits were in the ranges of 408.92-430.98 and 74.30-104.04 μmol Trolox equivalents (TE)/g dw, respectively. The number of identified phenolics was lower in fruits compared to leaves. Such a large number of bioactive phenolics identified and the strong antioxidant activity pointed to A. unedo as a promising health-promoting plant and natural food preservative.",
publisher = "Basel: MDPI",
journal = "Plants (Basel, Switzerland)",
title = "Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits.",
number = "1",
volume = "11",
doi = "10.3390/plants11010025",
pages = "25"
}

Brčić Karačonji, I., Jurica, K., Gašić, U., Dramićanin, A., Tešić, Ž.,& Milojković Opsenica, D.. (2022). Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits.. in Plants (Basel, Switzerland)
Basel: MDPI., 11(1), 25.
https://doi.org/10.3390/plants11010025

Brčić Karačonji I, Jurica K, Gašić U, Dramićanin A, Tešić Ž, Milojković Opsenica D. Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits.. in Plants (Basel, Switzerland). 2022;11(1):25.
doi:10.3390/plants11010025 .

Brčić Karačonji, Irena, Jurica, Karlo, Gašić, Uroš, Dramićanin, Aleksandra, Tešić, Živoslav, Milojković Opsenica, Dušanka, "Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits." in Plants (Basel, Switzerland), 11, no. 1 (2022):25,
https://doi.org/10.3390/plants11010025 . .

TY  - JOUR
AU  - Ljoljić Bilić, Vanja
AU  - Gašić, Uroš
AU  - Milojković-Opsenica, Dušanka
AU  - Nemet, Ivan
AU  - Rončević, Sanda
AU  - Kosalec, Ivan
AU  - Vuković Rodriguez, Jadranka
PY  - 2020
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1002/cbdv.202000280
UR  - http://www.ncbi.nlm.nih.gov/pubmed/2634273
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32634273
UR  - https://radar.ibiss.bg.ac.rs/123456789/3855
AB  - Erodium cicutarium is known for its total polyphenolic content, but this work reveals the first highly detailed profile of E. cicutarium, obtained with UHPLC-LTQ OrbiTrap MS4 and UHPLC-QqQ-MS/MS techniques. A total of 85 phenolic compounds were identified and 17 constituents were quantified. Overall, 25 new compounds were found, which have not yet been reported for the Erodium genera, or the family Geraniaceae. Along with methanolic extracts, the so far poorly investigated water extracts exhibited in vitro antioxidant activity according to all performed assays, including the ferric reducing/antioxidant power assay (FRAP), 2,2-diphenyl-1-picrylhydrazyl assay (DPPH), 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) assay (ABTS) and cupric ion reducing antioxidant capacity assay (CUPRAC). Elemental composition analysis performed with inductively coupled plasma atomic emission spectrometry (ICP-AES) and, additionally, hydride generation atomic absorption spectrometry (HydrEA-ETAAS) showed six most abundant elements to be decreasing as follows: Mg>Ca>K>S>P>Na, and gave first data regarding inorganic arsenic content (109.3-248.4 ng g-1 ). These results suggest E. cicutarium to be a valuable source of various phenolic compounds with substantial potential for further bioactivity testing.
PB  - Wiley-VCH Verlag
T2  - Chemistry & Biodiversity
T1  - First Extensive Polyphenolic Profile of Erodium cicutarium with Novel Insights to Elemental Composition and Antioxidant Activity.
VL  - 80
DO  - 10.1002/cbdv.202000280
SP  - cbdv.202000280
ER  - 

@article{
author = "Ljoljić Bilić, Vanja and Gašić, Uroš and Milojković-Opsenica, Dušanka and Nemet, Ivan and Rončević, Sanda and Kosalec, Ivan and Vuković Rodriguez, Jadranka",
year = "2020",
abstract = "Erodium cicutarium is known for its total polyphenolic content, but this work reveals the first highly detailed profile of E. cicutarium, obtained with UHPLC-LTQ OrbiTrap MS4 and UHPLC-QqQ-MS/MS techniques. A total of 85 phenolic compounds were identified and 17 constituents were quantified. Overall, 25 new compounds were found, which have not yet been reported for the Erodium genera, or the family Geraniaceae. Along with methanolic extracts, the so far poorly investigated water extracts exhibited in vitro antioxidant activity according to all performed assays, including the ferric reducing/antioxidant power assay (FRAP), 2,2-diphenyl-1-picrylhydrazyl assay (DPPH), 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) assay (ABTS) and cupric ion reducing antioxidant capacity assay (CUPRAC). Elemental composition analysis performed with inductively coupled plasma atomic emission spectrometry (ICP-AES) and, additionally, hydride generation atomic absorption spectrometry (HydrEA-ETAAS) showed six most abundant elements to be decreasing as follows: Mg>Ca>K>S>P>Na, and gave first data regarding inorganic arsenic content (109.3-248.4 ng g-1 ). These results suggest E. cicutarium to be a valuable source of various phenolic compounds with substantial potential for further bioactivity testing.",
publisher = "Wiley-VCH Verlag",
journal = "Chemistry & Biodiversity",
title = "First Extensive Polyphenolic Profile of Erodium cicutarium with Novel Insights to Elemental Composition and Antioxidant Activity.",
volume = "80",
doi = "10.1002/cbdv.202000280",
pages = "cbdv.202000280"
}

Ljoljić Bilić, V., Gašić, U., Milojković-Opsenica, D., Nemet, I., Rončević, S., Kosalec, I.,& Vuković Rodriguez, J.. (2020). First Extensive Polyphenolic Profile of Erodium cicutarium with Novel Insights to Elemental Composition and Antioxidant Activity.. in Chemistry & Biodiversity
Wiley-VCH Verlag., 80, cbdv.202000280.
https://doi.org/10.1002/cbdv.202000280

Ljoljić Bilić V, Gašić U, Milojković-Opsenica D, Nemet I, Rončević S, Kosalec I, Vuković Rodriguez J. First Extensive Polyphenolic Profile of Erodium cicutarium with Novel Insights to Elemental Composition and Antioxidant Activity.. in Chemistry & Biodiversity. 2020;80:cbdv.202000280.
doi:10.1002/cbdv.202000280 .

Ljoljić Bilić, Vanja, Gašić, Uroš, Milojković-Opsenica, Dušanka, Nemet, Ivan, Rončević, Sanda, Kosalec, Ivan, Vuković Rodriguez, Jadranka, "First Extensive Polyphenolic Profile of Erodium cicutarium with Novel Insights to Elemental Composition and Antioxidant Activity." in Chemistry & Biodiversity, 80 (2020):cbdv.202000280,
https://doi.org/10.1002/cbdv.202000280 . .

TY  - JOUR
AU  - Jurič, Andreja
AU  - Gašić, Uroš
AU  - Brčić Karačonji, Irena
AU  - Jurica, Karlo
AU  - Milojković-Opsenica, Dušanka
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3688
AB  - Despite of the many beneficial health effects of strawberry tree (Arbutus unedo L.) honey due to its strong antioxidant activity derived mostly from polyphenols, a detailed phenolic profile has not been previously studied. The aims of this study were to identify the phenolic compounds, determine the total phenolic content (TPC) and evaluate the radical scavenging activity (RSA) of strawberry tree honey from south Croatia. Fifty-two polyphenolics (twenty-seven phenolic acids and twenty-five flavonoids) were identified using ultra-high-performance liquid chromatograph (UHPLC) coupled to a hybrid mass spectrometer (LTQ Orbitrap MS). Our overall results point to higher TPC (1038 mg gallic acid equivalents per kg of honey) and stronger RSA (3.32 mmol Trolox equivalents per kg of honey) compared to other monofloral honeys. Due to the presence of large quantities of polyphenolic compounds, strawberry tree honey may have great potential as a health promoting food. Line
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - The phenolic profile of strawberry tree (Arbutus unedo L.) honey
IS  - 8
VL  - 85
DO  - 10.2298/JSC191217018J
SP  - 1011
EP  - 1019
ER  - 

@article{
author = "Jurič, Andreja and Gašić, Uroš and Brčić Karačonji, Irena and Jurica, Karlo and Milojković-Opsenica, Dušanka",
year = "2020",
abstract = "Despite of the many beneficial health effects of strawberry tree (Arbutus unedo L.) honey due to its strong antioxidant activity derived mostly from polyphenols, a detailed phenolic profile has not been previously studied. The aims of this study were to identify the phenolic compounds, determine the total phenolic content (TPC) and evaluate the radical scavenging activity (RSA) of strawberry tree honey from south Croatia. Fifty-two polyphenolics (twenty-seven phenolic acids and twenty-five flavonoids) were identified using ultra-high-performance liquid chromatograph (UHPLC) coupled to a hybrid mass spectrometer (LTQ Orbitrap MS). Our overall results point to higher TPC (1038 mg gallic acid equivalents per kg of honey) and stronger RSA (3.32 mmol Trolox equivalents per kg of honey) compared to other monofloral honeys. Due to the presence of large quantities of polyphenolic compounds, strawberry tree honey may have great potential as a health promoting food. Line",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "The phenolic profile of strawberry tree (Arbutus unedo L.) honey",
number = "8",
volume = "85",
doi = "10.2298/JSC191217018J",
pages = "1011-1019"
}

Jurič, A., Gašić, U., Brčić Karačonji, I., Jurica, K.,& Milojković-Opsenica, D.. (2020). The phenolic profile of strawberry tree (Arbutus unedo L.) honey. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 85(8), 1011-1019.
https://doi.org/10.2298/JSC191217018J

Jurič A, Gašić U, Brčić Karačonji I, Jurica K, Milojković-Opsenica D. The phenolic profile of strawberry tree (Arbutus unedo L.) honey. in Journal of the Serbian Chemical Society. 2020;85(8):1011-1019.
doi:10.2298/JSC191217018J .

Jurič, Andreja, Gašić, Uroš, Brčić Karačonji, Irena, Jurica, Karlo, Milojković-Opsenica, Dušanka, "The phenolic profile of strawberry tree (Arbutus unedo L.) honey" in Journal of the Serbian Chemical Society, 85, no. 8 (2020):1011-1019,
https://doi.org/10.2298/JSC191217018J . .

TY  - JOUR
AU  - Mosić, Mirjana
AU  - Trifković, Jelena
AU  - Vovk, Irena
AU  - Gašić, Uroš
AU  - Tešić, Živoslav
AU  - Šikoparija, Branko
AU  - Milojković-Opsenica, Dušanka
PY  - 2019
UR  - https://www.mdpi.com/2218-273X/9/12/783
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3549
AB  - Information on compositional, nutritional and functional properties of bee-pollen, as a health-promoting food, is essential for defining its quality. Concerning the nutritional importance of phenolic compounds, the aim of this study was to determine the phenolic profile and antioxidant activity of twenty-four bee-pollen samples collected from different regions of Serbia. High-performance thin-layer chromatographic (HPTLC) fingerprinting was used for profiling of bee-pollen samples according to the botanical type. HPTLC hyphenated with image analysis and a pattern recognition technique confirmed the grouping of samples caused by the specific phenolic composition of pollens of different botanical origin. Flavonoid glycosides in bee-pollen samples were identified by applying ultra-high-performance liquid chromatography (UHPLC) coupled with linear ion trap-Orbitrap mass spectrometry (LTQ Orbitrap MS). Eight out of twenty-seven flavonol glycosides were identified in bee-pollen samples for the first time. All analyzed bee-pollen samples showed a high number of phenolic compounds which may have therapeutic potential.
PB  - Multidisciplinary Digital Publishing Institute
T2  - Biomolecules
T2  - Biomolecules
T1  - Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen.
IS  - 12
VL  - 9
DO  - 10.3390/biom9120783
SP  - 783
ER  - 

@article{
author = "Mosić, Mirjana and Trifković, Jelena and Vovk, Irena and Gašić, Uroš and Tešić, Živoslav and Šikoparija, Branko and Milojković-Opsenica, Dušanka",
year = "2019",
abstract = "Information on compositional, nutritional and functional properties of bee-pollen, as a health-promoting food, is essential for defining its quality. Concerning the nutritional importance of phenolic compounds, the aim of this study was to determine the phenolic profile and antioxidant activity of twenty-four bee-pollen samples collected from different regions of Serbia. High-performance thin-layer chromatographic (HPTLC) fingerprinting was used for profiling of bee-pollen samples according to the botanical type. HPTLC hyphenated with image analysis and a pattern recognition technique confirmed the grouping of samples caused by the specific phenolic composition of pollens of different botanical origin. Flavonoid glycosides in bee-pollen samples were identified by applying ultra-high-performance liquid chromatography (UHPLC) coupled with linear ion trap-Orbitrap mass spectrometry (LTQ Orbitrap MS). Eight out of twenty-seven flavonol glycosides were identified in bee-pollen samples for the first time. All analyzed bee-pollen samples showed a high number of phenolic compounds which may have therapeutic potential.",
publisher = "Multidisciplinary Digital Publishing Institute",
journal = "Biomolecules, Biomolecules",
title = "Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen.",
number = "12",
volume = "9",
doi = "10.3390/biom9120783",
pages = "783"
}

Mosić, M., Trifković, J., Vovk, I., Gašić, U., Tešić, Ž., Šikoparija, B.,& Milojković-Opsenica, D.. (2019). Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen.. in Biomolecules
Multidisciplinary Digital Publishing Institute., 9(12), 783.
https://doi.org/10.3390/biom9120783

Mosić M, Trifković J, Vovk I, Gašić U, Tešić Ž, Šikoparija B, Milojković-Opsenica D. Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen.. in Biomolecules. 2019;9(12):783.
doi:10.3390/biom9120783 .

Mosić, Mirjana, Trifković, Jelena, Vovk, Irena, Gašić, Uroš, Tešić, Živoslav, Šikoparija, Branko, Milojković-Opsenica, Dušanka, "Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen." in Biomolecules, 9, no. 12 (2019):783,
https://doi.org/10.3390/biom9120783 . .