TY  - CONF
AU  - Kolarević, Stoimir
AU  - Micsinai, Adrienn
AU  - Szanto-Egesz, Reka
AU  - Lukacs, Alena
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Đorđević, Ana
AU  - Vojnović-Milutinović, Danijela
AU  - Kirschner, Alexander
AU  - Farnleitner, Andreas
AU  - Linke, Rita
AU  - Đukić, Aleksandar
AU  - Kostić, Jovana
AU  - Sunjog, Karolina
AU  - Paunović, Momir
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6693
AB  - Pollution of surface waters still represents one of the major environmental challenges in the Republic of Serbia, where wastewaters are discharged directly into the recipients without proper treatment. Within the last decade, we have assessed the microbiological water quality of more than 100 sites situated at different water bodies in Serbia. Quality was determined using faecal indicator bacteria, while microbial source tracking was employed to assess the source of pollution. Almost 50 % of the investigated sites were characterized by critical or even higher level of faecal contamination indicating that untreated wastewaters indeed represent significant pollution pressure on surface waters. Human-associated markers were prevalent in samples from the majority of contaminated sites but the source of pollution was not exclusively human-associated. 
The impact on the water quality of the Danube River was demonstrated in our previous research conducted at the whole river level within the Joint Danube Surveys. In the river stretch from Novi Sad to its confluence with the Velika Morava River, all the midstream samples were critically polluted. In this section, the highest level of pollution was recorded downstream of Belgrade. As ultimate recipients of wastewaters, Danube and its largest tributary Sava currently represent the only solution for disposing of wastewaters originating from the Serbian capital‘s 1,700,000 inhabitants. Such kind of disposal rises additional issues such as antimicrobial resistance and presence of infectious agents in water. For instance during COVID-19 pandemic, we have demonstrated that SARS-CoV-2 RNA can be detected even in surface waters of the Danube River at the sites receiving high wastewater loads from Belgrade which was the unique case for the Basin. 
Despites its extreme importance in this case, wastewater-based epidemiology is neglected in our country and hereby we would like to emphasize the need for implementation of program of such kind in Serbia.
PB  - Belgrade: Serbian Society for Microbiology
C3  - Book of abstracts: 13th Congress of Microbiologists of Serbia: Mikromed Regio 5: From biotechnology to human and planetary health; 2024 Apr 4-6; Belgrade, Serbia
T1  - Impact of untreated wastewaters on the microbiological water quality  of the Danube River  and its tributaries in Serbia
SP  - 65
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6693
ER  - 

@conference{
author = "Kolarević, Stoimir and Micsinai, Adrienn and Szanto-Egesz, Reka and Lukacs, Alena and Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Đorđević, Ana and Vojnović-Milutinović, Danijela and Kirschner, Alexander and Farnleitner, Andreas and Linke, Rita and Đukić, Aleksandar and Kostić, Jovana and Sunjog, Karolina and Paunović, Momir",
year = "2024",
abstract = "Pollution of surface waters still represents one of the major environmental challenges in the Republic of Serbia, where wastewaters are discharged directly into the recipients without proper treatment. Within the last decade, we have assessed the microbiological water quality of more than 100 sites situated at different water bodies in Serbia. Quality was determined using faecal indicator bacteria, while microbial source tracking was employed to assess the source of pollution. Almost 50 % of the investigated sites were characterized by critical or even higher level of faecal contamination indicating that untreated wastewaters indeed represent significant pollution pressure on surface waters. Human-associated markers were prevalent in samples from the majority of contaminated sites but the source of pollution was not exclusively human-associated. 
The impact on the water quality of the Danube River was demonstrated in our previous research conducted at the whole river level within the Joint Danube Surveys. In the river stretch from Novi Sad to its confluence with the Velika Morava River, all the midstream samples were critically polluted. In this section, the highest level of pollution was recorded downstream of Belgrade. As ultimate recipients of wastewaters, Danube and its largest tributary Sava currently represent the only solution for disposing of wastewaters originating from the Serbian capital‘s 1,700,000 inhabitants. Such kind of disposal rises additional issues such as antimicrobial resistance and presence of infectious agents in water. For instance during COVID-19 pandemic, we have demonstrated that SARS-CoV-2 RNA can be detected even in surface waters of the Danube River at the sites receiving high wastewater loads from Belgrade which was the unique case for the Basin. 
Despites its extreme importance in this case, wastewater-based epidemiology is neglected in our country and hereby we would like to emphasize the need for implementation of program of such kind in Serbia.",
publisher = "Belgrade: Serbian Society for Microbiology",
journal = "Book of abstracts: 13th Congress of Microbiologists of Serbia: Mikromed Regio 5: From biotechnology to human and planetary health; 2024 Apr 4-6; Belgrade, Serbia",
title = "Impact of untreated wastewaters on the microbiological water quality  of the Danube River  and its tributaries in Serbia",
pages = "65",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6693"
}

Kolarević, S., Micsinai, A., Szanto-Egesz, R., Lukacs, A., Kračun-Kolarević, M., Jovanović Marić, J., Đorđević, A., Vojnović-Milutinović, D., Kirschner, A., Farnleitner, A., Linke, R., Đukić, A., Kostić, J., Sunjog, K.,& Paunović, M.. (2024). Impact of untreated wastewaters on the microbiological water quality  of the Danube River  and its tributaries in Serbia. in Book of abstracts: 13th Congress of Microbiologists of Serbia: Mikromed Regio 5: From biotechnology to human and planetary health; 2024 Apr 4-6; Belgrade, Serbia
Belgrade: Serbian Society for Microbiology., 65.
https://hdl.handle.net/21.15107/rcub_ibiss_6693

Kolarević S, Micsinai A, Szanto-Egesz R, Lukacs A, Kračun-Kolarević M, Jovanović Marić J, Đorđević A, Vojnović-Milutinović D, Kirschner A, Farnleitner A, Linke R, Đukić A, Kostić J, Sunjog K, Paunović M. Impact of untreated wastewaters on the microbiological water quality  of the Danube River  and its tributaries in Serbia. in Book of abstracts: 13th Congress of Microbiologists of Serbia: Mikromed Regio 5: From biotechnology to human and planetary health; 2024 Apr 4-6; Belgrade, Serbia. 2024;:65.
https://hdl.handle.net/21.15107/rcub_ibiss_6693 .

Kolarević, Stoimir, Micsinai, Adrienn, Szanto-Egesz, Reka, Lukacs, Alena, Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Đorđević, Ana, Vojnović-Milutinović, Danijela, Kirschner, Alexander, Farnleitner, Andreas, Linke, Rita, Đukić, Aleksandar, Kostić, Jovana, Sunjog, Karolina, Paunović, Momir, "Impact of untreated wastewaters on the microbiological water quality  of the Danube River  and its tributaries in Serbia" in Book of abstracts: 13th Congress of Microbiologists of Serbia: Mikromed Regio 5: From biotechnology to human and planetary health; 2024 Apr 4-6; Belgrade, Serbia (2024):65,
https://hdl.handle.net/21.15107/rcub_ibiss_6693 .

TY  - CONF
AU  - Kolarević, Stoimir
AU  - Adrienn, Micsinai
AU  - Szanto-Egesz, Reka
AU  - Lukacs, Alena
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Đorđević, Ana
AU  - Vojnović-Milutinović, Danijela
AU  - Kirschner, Alexander
AU  - Farnleitner, Andreas
AU  - Linke, Rita
AU  - Đukić, Aleksandar
AU  - Kostić, Jovana
AU  - Sunjog, Karolina
AU  - Paunović, Momir
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6591
AB  - Pollution of natural water bodies is one of the biggest challenges in developing countries, such as
Serbia, where wastewater is discharged directly into the recipients without proper treatment and
poses serious threats to surface water quality, general safety and environmental health. Numerous
pathogenic microorganisms, bacteria, viruses, protozoa and helminthes may reach surface waters
and eventually contaminate groundwater. Despites its extreme importance in this case,
implementation of wastewater-based epidemiology is a challenge for developing countries, as the
majority of households are not connected to sewerage systems. Within our research, we have placed
major focus on recipients of wastewaters as an alternative of targeting raw wastewater. During the
fourth COVID-19 wave in Serbia that started in late February 2021 multiple water samples were
collected at 12 sites at Sava and Danube Rivers in the Belgrade city area which are under the impact
of untreated wastewaters.
SARS-CoV-2 RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2)
and envelope (E) protein genes. Microbiological (standard fecal indicator bacteria and microbial
faecal source tracking markers), epidemiological, physico-chemical and hydro-morphological
parameters were analysed in parallel. Out of 44 samples analyzed, 31 were positive for at least one
of the target regions of SARS-CoV-2. The results indicated that surveillance of SARS-CoV-2 RNA in
surface waters in context with the large amount of epidemiological and environmental metadata can
be used as epidemiological early-warning tool in countries with poor wastewater treatment.
PB  - Austrian Committee - International Association for Danube Research
C3  - Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
T1  - Applicability of wastewater based epidemiology in countries with poor wastewater treatment – COVID-19 case study in Serbia
SP  - 11
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6591
ER  - 

@conference{
author = "Kolarević, Stoimir and Adrienn, Micsinai and Szanto-Egesz, Reka and Lukacs, Alena and Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Đorđević, Ana and Vojnović-Milutinović, Danijela and Kirschner, Alexander and Farnleitner, Andreas and Linke, Rita and Đukić, Aleksandar and Kostić, Jovana and Sunjog, Karolina and Paunović, Momir",
year = "2023",
abstract = "Pollution of natural water bodies is one of the biggest challenges in developing countries, such as
Serbia, where wastewater is discharged directly into the recipients without proper treatment and
poses serious threats to surface water quality, general safety and environmental health. Numerous
pathogenic microorganisms, bacteria, viruses, protozoa and helminthes may reach surface waters
and eventually contaminate groundwater. Despites its extreme importance in this case,
implementation of wastewater-based epidemiology is a challenge for developing countries, as the
majority of households are not connected to sewerage systems. Within our research, we have placed
major focus on recipients of wastewaters as an alternative of targeting raw wastewater. During the
fourth COVID-19 wave in Serbia that started in late February 2021 multiple water samples were
collected at 12 sites at Sava and Danube Rivers in the Belgrade city area which are under the impact
of untreated wastewaters.
SARS-CoV-2 RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2)
and envelope (E) protein genes. Microbiological (standard fecal indicator bacteria and microbial
faecal source tracking markers), epidemiological, physico-chemical and hydro-morphological
parameters were analysed in parallel. Out of 44 samples analyzed, 31 were positive for at least one
of the target regions of SARS-CoV-2. The results indicated that surveillance of SARS-CoV-2 RNA in
surface waters in context with the large amount of epidemiological and environmental metadata can
be used as epidemiological early-warning tool in countries with poor wastewater treatment.",
publisher = "Austrian Committee - International Association for Danube Research",
journal = "Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria",
title = "Applicability of wastewater based epidemiology in countries with poor wastewater treatment – COVID-19 case study in Serbia",
pages = "11",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6591"
}

Kolarević, S., Adrienn, M., Szanto-Egesz, R., Lukacs, A., Kračun-Kolarević, M., Jovanović Marić, J., Đorđević, A., Vojnović-Milutinović, D., Kirschner, A., Farnleitner, A., Linke, R., Đukić, A., Kostić, J., Sunjog, K.,& Paunović, M.. (2023). Applicability of wastewater based epidemiology in countries with poor wastewater treatment – COVID-19 case study in Serbia. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
Austrian Committee - International Association for Danube Research., 11.
https://hdl.handle.net/21.15107/rcub_ibiss_6591

Kolarević S, Adrienn M, Szanto-Egesz R, Lukacs A, Kračun-Kolarević M, Jovanović Marić J, Đorđević A, Vojnović-Milutinović D, Kirschner A, Farnleitner A, Linke R, Đukić A, Kostić J, Sunjog K, Paunović M. Applicability of wastewater based epidemiology in countries with poor wastewater treatment – COVID-19 case study in Serbia. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria. 2023;:11.
https://hdl.handle.net/21.15107/rcub_ibiss_6591 .

Kolarević, Stoimir, Adrienn, Micsinai, Szanto-Egesz, Reka, Lukacs, Alena, Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Đorđević, Ana, Vojnović-Milutinović, Danijela, Kirschner, Alexander, Farnleitner, Andreas, Linke, Rita, Đukić, Aleksandar, Kostić, Jovana, Sunjog, Karolina, Paunović, Momir, "Applicability of wastewater based epidemiology in countries with poor wastewater treatment – COVID-19 case study in Serbia" in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria (2023):11,
https://hdl.handle.net/21.15107/rcub_ibiss_6591 .

TY  - JOUR
AU  - Takodi, Nada
AU  - Drobac Backović, Damjana
AU  - Marinović, Zoran
AU  - Sunjog, Karolina
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Kitanović, Nevena
AU  - Vuković-Gačić, Branka
AU  - Lujić, Jelena
AU  - Svirčev, Zorica
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6464
AB  - Lake Ludaš is a protected nature reserve in Serbia that has been the focus of scientific interest, due to
perpetual water quality deterioration that has led to consistent cyanobacterial blooms for almost five decades. To describe the possible effects of cyanotoxins on fish (Carrasius gibelio) inhabiting Lake Ludaš, several examinations were performed in November of 2018, including: assessment of water quality (physical and chemical parameters, as well as water microbiology), and assessment of several tissues of C. gibelio (micronucleus test, comet assay, and histopathological analyses). The water quality of Lake Ludaš has been categorized as class III, indicating moderate pollution. Physical and chemical parameters from November of 2018 seemed to be within acceptable levels. Examined fish tissues showed signs of DNA damage (highest in the blood, followed by the liver and gills), and histological alterations (in the liver
and gills). Although other xenobiotics can induce similar changes, cyanotoxins could be the most likely cause of the observed alterations in C. gibelio tissues. The present study demonstrates the need to monitor this important wetland of international significance, since the living organisms in Lake Ludaš are showing signs of environmental stress suggesting that the preservation and natural balance of this ecosystem could be in danger.
PB  - Novi Sad : Faculty of Sciences, Department of Biology and Ecology
T2  - Biologia Serbica
T1  - Harmful effects of cyanobacterial blooms in Lake Ludaš (Serbia) on Carassius gibelio tissues
IS  - 1
VL  - 45
DO  - 10.5281/zenodo.8304662
SP  - 3
EP  - 9
ER  - 

@article{
author = "Takodi, Nada and Drobac Backović, Damjana and Marinović, Zoran and Sunjog, Karolina and Jovanović Marić, Jovana and Kolarević, Stoimir and Kitanović, Nevena and Vuković-Gačić, Branka and Lujić, Jelena and Svirčev, Zorica",
year = "2023",
abstract = "Lake Ludaš is a protected nature reserve in Serbia that has been the focus of scientific interest, due to
perpetual water quality deterioration that has led to consistent cyanobacterial blooms for almost five decades. To describe the possible effects of cyanotoxins on fish (Carrasius gibelio) inhabiting Lake Ludaš, several examinations were performed in November of 2018, including: assessment of water quality (physical and chemical parameters, as well as water microbiology), and assessment of several tissues of C. gibelio (micronucleus test, comet assay, and histopathological analyses). The water quality of Lake Ludaš has been categorized as class III, indicating moderate pollution. Physical and chemical parameters from November of 2018 seemed to be within acceptable levels. Examined fish tissues showed signs of DNA damage (highest in the blood, followed by the liver and gills), and histological alterations (in the liver
and gills). Although other xenobiotics can induce similar changes, cyanotoxins could be the most likely cause of the observed alterations in C. gibelio tissues. The present study demonstrates the need to monitor this important wetland of international significance, since the living organisms in Lake Ludaš are showing signs of environmental stress suggesting that the preservation and natural balance of this ecosystem could be in danger.",
publisher = "Novi Sad : Faculty of Sciences, Department of Biology and Ecology",
journal = "Biologia Serbica",
title = "Harmful effects of cyanobacterial blooms in Lake Ludaš (Serbia) on Carassius gibelio tissues",
number = "1",
volume = "45",
doi = "10.5281/zenodo.8304662",
pages = "3-9"
}

Takodi, N., Drobac Backović, D., Marinović, Z., Sunjog, K., Jovanović Marić, J., Kolarević, S., Kitanović, N., Vuković-Gačić, B., Lujić, J.,& Svirčev, Z.. (2023). Harmful effects of cyanobacterial blooms in Lake Ludaš (Serbia) on Carassius gibelio tissues. in Biologia Serbica
Novi Sad : Faculty of Sciences, Department of Biology and Ecology., 45(1), 3-9.
https://doi.org/10.5281/zenodo.8304662

Takodi N, Drobac Backović D, Marinović Z, Sunjog K, Jovanović Marić J, Kolarević S, Kitanović N, Vuković-Gačić B, Lujić J, Svirčev Z. Harmful effects of cyanobacterial blooms in Lake Ludaš (Serbia) on Carassius gibelio tissues. in Biologia Serbica. 2023;45(1):3-9.
doi:10.5281/zenodo.8304662 .

Takodi, Nada, Drobac Backović, Damjana, Marinović, Zoran, Sunjog, Karolina, Jovanović Marić, Jovana, Kolarević, Stoimir, Kitanović, Nevena, Vuković-Gačić, Branka, Lujić, Jelena, Svirčev, Zorica, "Harmful effects of cyanobacterial blooms in Lake Ludaš (Serbia) on Carassius gibelio tissues" in Biologia Serbica, 45, no. 1 (2023):3-9,
https://doi.org/10.5281/zenodo.8304662 . .

TY  - JOUR
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Đorđević, Jelena
AU  - Sunjog, Karolina
AU  - Nikolić, Ivan
AU  - Marić, Ana
AU  - Ilić, Marija
AU  - Simonović, Predrag
AU  - Alygizakis, Nikiforos
AU  - Ng, Kelsey
AU  - Oswald, Peter
AU  - Slobodnik, Jaroslav
AU  - Žegura, Bojana
AU  - Vuković-Gačić, Branka
AU  - Paunović, Momir
AU  - Kračun-Kolarević, Margareta
PY  - 2023
UR  - https://academic.oup.com/mutage/advance-article/doi/10.1093/mutage/geac024/6823671
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5243
AB  - Environmental studies which aim to assess the ecological impact of chemical and other types of pollution should employ a complex weight-of-evidence approach with multiple lines of evidence (LoEs). This study focused on in situ genotoxicological methods such as the comet and micronucleus assays and randomly amplified polymorphic DNA analysis as one of the multiple LoEs (LoE3) on the fish species Alburnus alburnus (bleak) as a bioindicator. The study was carried out within the Joint Danube Survey 4 (JDS4) at nine sites in the Danube River Basin in the Republic of Serbia. Out of nine sampling sites, two were situated at the Tisa, Sava, and Velika Morava rivers, and three sites were at the Danube River. The three additionally employed LoEs were: SumTUwater calculated based on the monitoring data in the database of the Serbian Environmental Protection Agency (SEPA) (LoE1); in vitro analyses of JDS4 water extracts employing genotoxicological methods (LoE2); assessment of the ecological status/potential by SEPA and indication of the ecological status for the sites performed within the JDS4 (LoE4). The analyzed biomarker responses in the bleak were integrated into the unique integrated biomarker response index which was used to rank the sites. The highest pollution pressure was recorded at JDS4 39 and JDS4 36, while the lowest was at JDS4 35. The impact of pollution was confirmed at three sites, JDS4 33, 40, and 41, by all four LoEs. At other sampling sites, a difference was observed regarding the pollution depending on the employed LoEs. This indicates the importance of implementing a comprehensive weight-of-evidence approach to ensure the impact of pollution is not overlooked when using only one LoE as is often the case in environmental studies.
PB  - Oxford: Oxford University Press
T2  - Mutagenesis
T1  - In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study
IS  - 1
VL  - 38
DO  - 10.1093/mutage/geac024
SP  - 21
EP  - 32
ER  - 

@article{
author = "Jovanović Marić, Jovana and Kolarević, Stoimir and Đorđević, Jelena and Sunjog, Karolina and Nikolić, Ivan and Marić, Ana and Ilić, Marija and Simonović, Predrag and Alygizakis, Nikiforos and Ng, Kelsey and Oswald, Peter and Slobodnik, Jaroslav and Žegura, Bojana and Vuković-Gačić, Branka and Paunović, Momir and Kračun-Kolarević, Margareta",
year = "2023",
abstract = "Environmental studies which aim to assess the ecological impact of chemical and other types of pollution should employ a complex weight-of-evidence approach with multiple lines of evidence (LoEs). This study focused on in situ genotoxicological methods such as the comet and micronucleus assays and randomly amplified polymorphic DNA analysis as one of the multiple LoEs (LoE3) on the fish species Alburnus alburnus (bleak) as a bioindicator. The study was carried out within the Joint Danube Survey 4 (JDS4) at nine sites in the Danube River Basin in the Republic of Serbia. Out of nine sampling sites, two were situated at the Tisa, Sava, and Velika Morava rivers, and three sites were at the Danube River. The three additionally employed LoEs were: SumTUwater calculated based on the monitoring data in the database of the Serbian Environmental Protection Agency (SEPA) (LoE1); in vitro analyses of JDS4 water extracts employing genotoxicological methods (LoE2); assessment of the ecological status/potential by SEPA and indication of the ecological status for the sites performed within the JDS4 (LoE4). The analyzed biomarker responses in the bleak were integrated into the unique integrated biomarker response index which was used to rank the sites. The highest pollution pressure was recorded at JDS4 39 and JDS4 36, while the lowest was at JDS4 35. The impact of pollution was confirmed at three sites, JDS4 33, 40, and 41, by all four LoEs. At other sampling sites, a difference was observed regarding the pollution depending on the employed LoEs. This indicates the importance of implementing a comprehensive weight-of-evidence approach to ensure the impact of pollution is not overlooked when using only one LoE as is often the case in environmental studies.",
publisher = "Oxford: Oxford University Press",
journal = "Mutagenesis",
title = "In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study",
number = "1",
volume = "38",
doi = "10.1093/mutage/geac024",
pages = "21-32"
}

Jovanović Marić, J., Kolarević, S., Đorđević, J., Sunjog, K., Nikolić, I., Marić, A., Ilić, M., Simonović, P., Alygizakis, N., Ng, K., Oswald, P., Slobodnik, J., Žegura, B., Vuković-Gačić, B., Paunović, M.,& Kračun-Kolarević, M.. (2023). In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study. in Mutagenesis
Oxford: Oxford University Press., 38(1), 21-32.
https://doi.org/10.1093/mutage/geac024

Jovanović Marić J, Kolarević S, Đorđević J, Sunjog K, Nikolić I, Marić A, Ilić M, Simonović P, Alygizakis N, Ng K, Oswald P, Slobodnik J, Žegura B, Vuković-Gačić B, Paunović M, Kračun-Kolarević M. In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study. in Mutagenesis. 2023;38(1):21-32.
doi:10.1093/mutage/geac024 .

Jovanović Marić, Jovana, Kolarević, Stoimir, Đorđević, Jelena, Sunjog, Karolina, Nikolić, Ivan, Marić, Ana, Ilić, Marija, Simonović, Predrag, Alygizakis, Nikiforos, Ng, Kelsey, Oswald, Peter, Slobodnik, Jaroslav, Žegura, Bojana, Vuković-Gačić, Branka, Paunović, Momir, Kračun-Kolarević, Margareta, "In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study" in Mutagenesis, 38, no. 1 (2023):21-32,
https://doi.org/10.1093/mutage/geac024 . .

TY  - CONF
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Kolarević, Stoimir
AU  - Smederevac-Lalić, Marija
AU  - Marković, Zoran
AU  - Skorić, Stefan
AU  - Jaćimović, Milica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5723
AB  - Invasive alien fish species represent one of the greatest threats to the
biodiversity and ecological status of freshwater ecosystems. Within their
management, their application as bioindicators of pollution in monitoring
programs should be considered. The aim of this study was to examine the
use of black bullhead Ameiurus melas (Rafinesque, 1820), invasive alien fish
in Serbia, as a bioindicator of water genotoxicity by alkaline comet assay.
Sampling was performed from June to September 2021 on Markovačko
Lake, which is exposed to diffuse pollution from the application of pesticides
on the surrounding agricultural land. Monitoring also included the
assessment of water quality by basic physical and chemical parameters, as
well as microbiological indicators of faecal pollution. Alkaline comet assay
was performed on fish erythrocytes, on ten specimens per month. The study
also included ten control fish from the recirculating aquaculture system
(RAS), which were transferred from Markovačko Lake for breeding, and
treatment of their erythrocytes with H2O2 as a positive control. Scoring of
comets was performed by CometScore 2.0 software. The lowest level of
DNA damage was recorded in the control group and the highest in the
positive control group. August was the month with the lowest level of DNA
damage, and September with the highest. Statistically significant
differences were found between all groups indicating a high sensitivity of
the alkaline comet assay to discriminate fine differences in the genotoxic
potential of water. Knowing that black bullhead is highly abundant in
stagnant waters and relatively easy to sample, this study draws additional
attention to its use as a bioindicator of genotoxicity.
PB  - European Environmental Mutagenesis & Genomics Society
C3  - Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands
T1  - Sensitivity of invasive alien fish in Serbia black bullhead Ameiurus melas (Rafinesque, 1820) as a bioindicator of genotoxicity.
SP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5723
ER  - 

@conference{
author = "Kostić-Vuković, Jovana and Sunjog, Karolina and Kolarević, Stoimir and Smederevac-Lalić, Marija and Marković, Zoran and Skorić, Stefan and Jaćimović, Milica",
year = "2022",
abstract = "Invasive alien fish species represent one of the greatest threats to the
biodiversity and ecological status of freshwater ecosystems. Within their
management, their application as bioindicators of pollution in monitoring
programs should be considered. The aim of this study was to examine the
use of black bullhead Ameiurus melas (Rafinesque, 1820), invasive alien fish
in Serbia, as a bioindicator of water genotoxicity by alkaline comet assay.
Sampling was performed from June to September 2021 on Markovačko
Lake, which is exposed to diffuse pollution from the application of pesticides
on the surrounding agricultural land. Monitoring also included the
assessment of water quality by basic physical and chemical parameters, as
well as microbiological indicators of faecal pollution. Alkaline comet assay
was performed on fish erythrocytes, on ten specimens per month. The study
also included ten control fish from the recirculating aquaculture system
(RAS), which were transferred from Markovačko Lake for breeding, and
treatment of their erythrocytes with H2O2 as a positive control. Scoring of
comets was performed by CometScore 2.0 software. The lowest level of
DNA damage was recorded in the control group and the highest in the
positive control group. August was the month with the lowest level of DNA
damage, and September with the highest. Statistically significant
differences were found between all groups indicating a high sensitivity of
the alkaline comet assay to discriminate fine differences in the genotoxic
potential of water. Knowing that black bullhead is highly abundant in
stagnant waters and relatively easy to sample, this study draws additional
attention to its use as a bioindicator of genotoxicity.",
publisher = "European Environmental Mutagenesis & Genomics Society",
journal = "Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands",
title = "Sensitivity of invasive alien fish in Serbia black bullhead Ameiurus melas (Rafinesque, 1820) as a bioindicator of genotoxicity.",
pages = "73",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5723"
}

Kostić-Vuković, J., Sunjog, K., Kolarević, S., Smederevac-Lalić, M., Marković, Z., Skorić, S.,& Jaćimović, M.. (2022). Sensitivity of invasive alien fish in Serbia black bullhead Ameiurus melas (Rafinesque, 1820) as a bioindicator of genotoxicity.. in Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands
European Environmental Mutagenesis & Genomics Society., 73.
https://hdl.handle.net/21.15107/rcub_ibiss_5723

Kostić-Vuković J, Sunjog K, Kolarević S, Smederevac-Lalić M, Marković Z, Skorić S, Jaćimović M. Sensitivity of invasive alien fish in Serbia black bullhead Ameiurus melas (Rafinesque, 1820) as a bioindicator of genotoxicity.. in Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands. 2022;:73.
https://hdl.handle.net/21.15107/rcub_ibiss_5723 .

Kostić-Vuković, Jovana, Sunjog, Karolina, Kolarević, Stoimir, Smederevac-Lalić, Marija, Marković, Zoran, Skorić, Stefan, Jaćimović, Milica, "Sensitivity of invasive alien fish in Serbia black bullhead Ameiurus melas (Rafinesque, 1820) as a bioindicator of genotoxicity." in Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands (2022):73,
https://hdl.handle.net/21.15107/rcub_ibiss_5723 .

TY  - CONF
AU  - Kolarević, Stoimir
AU  - Micsinai, Adrienn
AU  - Szántó-Egész, Réka
AU  - Lukács, Alena
AU  - Kračun-Kolarević, Margareta
AU  - Đorđević, Ana
AU  - Jovanović Marić, Jovana
AU  - Vojnović-Milutinović, Danijela
AU  - Kirschner, Alexander K.T.
AU  - Farnleitner, Andreas H.
AU  - Linke, Rita
AU  - Đukić, Aleksandar
AU  - Kostić, Jovana
AU  - Sunjog, Karolina
AU  - Paunović, Momir
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6235
AB  - BACKGROUND
Prokaryotic assays are of great interest in eco/geno-toxicology as they are short-term, simple and cost effective. Application of strains of Salmonella typhimurium with modifications such as increased cell wall permeability and lack of excision repair system is common for the detection of genotoxicity. Various eukaryotic models have been developed lately, such as fish derived cell lines, which can mimic the response of the aquatic organisms. Considering different level of cell organization among these models, difference in sensitivity is expected, yet only few studies address this topic.
OBJECTIVES
The major objective was to investigate genotoxic potential of extracts of surface water of the Danube
and to compare the results obtained in applied prokaryotic and eukaryotic models.
METHODS
Surface water samples from 24 sites along the Danube River were extracted by LVSPE Horizon sampler providing relative enrichment factor (REF) of the extracts of 25,000x. For representative prokaryotic model the SOS/umuC assay with S. typhimurium TA1535/pSK1002 was used. Eukaryotic model comprised assessment of cytotoxicity by MTS test and assessment of genotoxicity by comet assay in ZFL (ZebraFish Liver) cells.
RESULTS
While none of the tested samples has induced SOS response, the result of MTS assay indicated that 11 of 24 samples were cytotoxic. In comet assay, genotoxic potential was found in 9 of 24 samples. Results indicated higher sensitivity of used eukaryotic model in comparison with prokaryotic. Applied prokaryotic assay is often suggested to be used as prescreening tool in diagnostics of genotoxic potential which by our opinion could be misleading.
PB  - Belgrade: Serbian Society of Microbiology
C3  - Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia
T1  - Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters
SP  - 614
EP  - 615
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6235
ER  - 

@conference{
author = "Kolarević, Stoimir and Micsinai, Adrienn and Szántó-Egész, Réka and Lukács, Alena and Kračun-Kolarević, Margareta and Đorđević, Ana and Jovanović Marić, Jovana and Vojnović-Milutinović, Danijela and Kirschner, Alexander K.T. and Farnleitner, Andreas H. and Linke, Rita and Đukić, Aleksandar and Kostić, Jovana and Sunjog, Karolina and Paunović, Momir",
year = "2022",
abstract = "BACKGROUND
Prokaryotic assays are of great interest in eco/geno-toxicology as they are short-term, simple and cost effective. Application of strains of Salmonella typhimurium with modifications such as increased cell wall permeability and lack of excision repair system is common for the detection of genotoxicity. Various eukaryotic models have been developed lately, such as fish derived cell lines, which can mimic the response of the aquatic organisms. Considering different level of cell organization among these models, difference in sensitivity is expected, yet only few studies address this topic.
OBJECTIVES
The major objective was to investigate genotoxic potential of extracts of surface water of the Danube
and to compare the results obtained in applied prokaryotic and eukaryotic models.
METHODS
Surface water samples from 24 sites along the Danube River were extracted by LVSPE Horizon sampler providing relative enrichment factor (REF) of the extracts of 25,000x. For representative prokaryotic model the SOS/umuC assay with S. typhimurium TA1535/pSK1002 was used. Eukaryotic model comprised assessment of cytotoxicity by MTS test and assessment of genotoxicity by comet assay in ZFL (ZebraFish Liver) cells.
RESULTS
While none of the tested samples has induced SOS response, the result of MTS assay indicated that 11 of 24 samples were cytotoxic. In comet assay, genotoxic potential was found in 9 of 24 samples. Results indicated higher sensitivity of used eukaryotic model in comparison with prokaryotic. Applied prokaryotic assay is often suggested to be used as prescreening tool in diagnostics of genotoxic potential which by our opinion could be misleading.",
publisher = "Belgrade: Serbian Society of Microbiology",
journal = "Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia",
title = "Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters",
pages = "614-615",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6235"
}

Kolarević, S., Micsinai, A., Szántó-Egész, R., Lukács, A., Kračun-Kolarević, M., Đorđević, A., Jovanović Marić, J., Vojnović-Milutinović, D., Kirschner, A. K.T., Farnleitner, A. H., Linke, R., Đukić, A., Kostić, J., Sunjog, K.,& Paunović, M.. (2022). Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters. in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia
Belgrade: Serbian Society of Microbiology., 614-615.
https://hdl.handle.net/21.15107/rcub_ibiss_6235

Kolarević S, Micsinai A, Szántó-Egész R, Lukács A, Kračun-Kolarević M, Đorđević A, Jovanović Marić J, Vojnović-Milutinović D, Kirschner AK, Farnleitner AH, Linke R, Đukić A, Kostić J, Sunjog K, Paunović M. Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters. in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia. 2022;:614-615.
https://hdl.handle.net/21.15107/rcub_ibiss_6235 .

Kolarević, Stoimir, Micsinai, Adrienn, Szántó-Egész, Réka, Lukács, Alena, Kračun-Kolarević, Margareta, Đorđević, Ana, Jovanović Marić, Jovana, Vojnović-Milutinović, Danijela, Kirschner, Alexander K.T., Farnleitner, Andreas H., Linke, Rita, Đukić, Aleksandar, Kostić, Jovana, Sunjog, Karolina, Paunović, Momir, "Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters" in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia (2022):614-615,
https://hdl.handle.net/21.15107/rcub_ibiss_6235 .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Kračun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Đorđević, Jelena
AU  - Sunjog, Karolina
AU  - Kostić-Vuković, Jovana
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5727
AB  - U Srbiji se prerađuje manje od 13% otpadnih voda,1 što za posledicu ima
ispuštanje velikih količina neprečišćenih voda u vodotokove. Ravničarske reke
su pod značajnim pritiscima ovih izvora zagađenja, s obzirom da delom ili
čitavim tokom protiču kroz naseljene oblasti, kao i oblasti u kojima se nalaze
industrijska postrojenja. U analizi uticaja zagađenja na akvatične organizme,
oštećenje DNК molekula predstavlja značajan biomarker ranog upozorenja jer
može ukazati na uticaj ksenobiotika na biološke sisteme pre nego što se odrazi
na čitav organizam i/ili populaciju. U skladu sa tim, cilj ovog istraživanja je
procena osetljivosti široko rasprostranjene vrste Alburnus alburnus (uklija)
prilikom detekcije genotoksičnog efekta, kako na prostorno bliskim
lokalitetima na Savi, tako na i prostorno udaljenim na Dunavu, Tisi, Savi i
Velikoj Moravi. Za poređenje lokaliteta korišćen je integrisani odgovor
biomarkera (IBR) koji je objedinio pojedinačne biomarkere: vijabilnost ćelija
krvi, nivo DNК oštećenja ćelija krvi detektovanog u alkalnom komet i
mikronukleus testu i indeks zagađenja metalima/prioritetnim supstancama.
Integrisanjem pojedinačnih odgovora biomarkera u jedinstvenu IBR vrednost
omogućeno je jednostavnije poređenje lokaliteta. Dobijeni rezultati su ukazali da
su odgovori biomarkera uklija specifični za lokalitet. U skladu sa rezultatima,
utvrđeno je da uklija može biti pouzdan bioindikator u diskriminaciji i
udaljenih i prostorno bliskih lokaliteta koji su pod različitim uticajem
zagađenja.
AB  - У Србији се прерађује мање од 13% отпадних вода,1 што за последицу има
испуштање великих количина непречишћених вода у водотокове. Равничарске реке
су под значајним притисцима ових извора загађења, с обзиром да делом или
читавим током протичу кроз насељене области, као и области у којима се налазе
индустријска постројења. У анализи утицаја загађења на акватичне организме,
оштећење ДНК молекула представља значајан биомаркер раног упозорења јер
може указати на утицај ксенобиотика на биолошке системе пре него што се одрази
на читав организам и/или популацију. У складу са тим, циљ овог истраживања је
процена осетљивости широко распрострањене врсте Alburnus alburnus (уклија)
приликом детекције генотоксичног ефекта, како на просторно блиским
локалитетима на Сави, тако на и просторно удаљеним на Дунаву, Тиси, Сави и
Великој Морави. За поређење локалитета коришћен је интегрисани одговор
биомаркера (ИБР) који је објединио појединачне биомаркере: вијабилност ћелија
крви, ниво ДНК оштећења ћелија крви детектованог у алкалном комет и
микронуклеус тесту и индекс загађења металима/приоритетним супстанцама.
Интегрисањем појединачних одговора биомаркера у јединствену ИБР вредност
омогућено је једноставније поређење локалитета. Добијени резултати су указали да
су одговори биомаркера уклија специфични за локалитет. У складу са резултатима,
утврђено је да уклија може бити поуздан биоиндикатор у дискриминацији и
удаљених и просторно блиских локалитета који су под различитим утицајем
загађења.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Ispitivanje osetlјivosti vrste Alburnus alburnus (uklija) u ekogenotoksikološkim istraživanjima velikih ravničarskih reka.
T1  - Испитивање осетљивости врсте Alburnus alburnus (уклија) у екогенотоксиколошким истраживањима великих равничарских река
SP  - 155
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5727
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Kračun-Kolarević, Margareta and Kolarević, Stoimir and Đorđević, Jelena and Sunjog, Karolina and Kostić-Vuković, Jovana and Paunović, Momir and Vuković-Gačić, Branka",
year = "2022",
abstract = "U Srbiji se prerađuje manje od 13% otpadnih voda,1 što za posledicu ima
ispuštanje velikih količina neprečišćenih voda u vodotokove. Ravničarske reke
su pod značajnim pritiscima ovih izvora zagađenja, s obzirom da delom ili
čitavim tokom protiču kroz naseljene oblasti, kao i oblasti u kojima se nalaze
industrijska postrojenja. U analizi uticaja zagađenja na akvatične organizme,
oštećenje DNК molekula predstavlja značajan biomarker ranog upozorenja jer
može ukazati na uticaj ksenobiotika na biološke sisteme pre nego što se odrazi
na čitav organizam i/ili populaciju. U skladu sa tim, cilj ovog istraživanja je
procena osetljivosti široko rasprostranjene vrste Alburnus alburnus (uklija)
prilikom detekcije genotoksičnog efekta, kako na prostorno bliskim
lokalitetima na Savi, tako na i prostorno udaljenim na Dunavu, Tisi, Savi i
Velikoj Moravi. Za poređenje lokaliteta korišćen je integrisani odgovor
biomarkera (IBR) koji je objedinio pojedinačne biomarkere: vijabilnost ćelija
krvi, nivo DNК oštećenja ćelija krvi detektovanog u alkalnom komet i
mikronukleus testu i indeks zagađenja metalima/prioritetnim supstancama.
Integrisanjem pojedinačnih odgovora biomarkera u jedinstvenu IBR vrednost
omogućeno je jednostavnije poređenje lokaliteta. Dobijeni rezultati su ukazali da
su odgovori biomarkera uklija specifični za lokalitet. U skladu sa rezultatima,
utvrđeno je da uklija može biti pouzdan bioindikator u diskriminaciji i
udaljenih i prostorno bliskih lokaliteta koji su pod različitim uticajem
zagađenja., У Србији се прерађује мање од 13% отпадних вода,1 што за последицу има
испуштање великих количина непречишћених вода у водотокове. Равничарске реке
су под значајним притисцима ових извора загађења, с обзиром да делом или
читавим током протичу кроз насељене области, као и области у којима се налазе
индустријска постројења. У анализи утицаја загађења на акватичне организме,
оштећење ДНК молекула представља значајан биомаркер раног упозорења јер
може указати на утицај ксенобиотика на биолошке системе пре него што се одрази
на читав организам и/или популацију. У складу са тим, циљ овог истраживања је
процена осетљивости широко распрострањене врсте Alburnus alburnus (уклија)
приликом детекције генотоксичног ефекта, како на просторно блиским
локалитетима на Сави, тако на и просторно удаљеним на Дунаву, Тиси, Сави и
Великој Морави. За поређење локалитета коришћен је интегрисани одговор
биомаркера (ИБР) који је објединио појединачне биомаркере: вијабилност ћелија
крви, ниво ДНК оштећења ћелија крви детектованог у алкалном комет и
микронуклеус тесту и индекс загађења металима/приоритетним супстанцама.
Интегрисањем појединачних одговора биомаркера у јединствену ИБР вредност
омогућено је једноставније поређење локалитета. Добијени резултати су указали да
су одговори биомаркера уклија специфични за локалитет. У складу са резултатима,
утврђено је да уклија може бити поуздан биоиндикатор у дискриминацији и
удаљених и просторно блиских локалитета који су под различитим утицајем
загађења.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Ispitivanje osetlјivosti vrste Alburnus alburnus (uklija) u ekogenotoksikološkim istraživanjima velikih ravničarskih reka., Испитивање осетљивости врсте Alburnus alburnus (уклија) у екогенотоксиколошким истраживањима великих равничарских река",
pages = "155",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5727"
}

Jovanović Marić, J., Kračun-Kolarević, M., Kolarević, S., Đorđević, J., Sunjog, K., Kostić-Vuković, J., Paunović, M.,& Vuković-Gačić, B.. (2022). Ispitivanje osetlјivosti vrste Alburnus alburnus (uklija) u ekogenotoksikološkim istraživanjima velikih ravničarskih reka.. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 155.
https://hdl.handle.net/21.15107/rcub_ibiss_5727

Jovanović Marić J, Kračun-Kolarević M, Kolarević S, Đorđević J, Sunjog K, Kostić-Vuković J, Paunović M, Vuković-Gačić B. Ispitivanje osetlјivosti vrste Alburnus alburnus (uklija) u ekogenotoksikološkim istraživanjima velikih ravničarskih reka.. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:155.
https://hdl.handle.net/21.15107/rcub_ibiss_5727 .

Jovanović Marić, Jovana, Kračun-Kolarević, Margareta, Kolarević, Stoimir, Đorđević, Jelena, Sunjog, Karolina, Kostić-Vuković, Jovana, Paunović, Momir, Vuković-Gačić, Branka, "Ispitivanje osetlјivosti vrste Alburnus alburnus (uklija) u ekogenotoksikološkim istraživanjima velikih ravničarskih reka." in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):155,
https://hdl.handle.net/21.15107/rcub_ibiss_5727 .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Kračun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Nikolić, Ivan
AU  - Sunjog, Karolina
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4735
AB  - Metoda nasumično umnožene polimorfne DNK (RAPD) je poslednjih godina našla primenu u ekogenotoksikologiji. U okviru dve studije slučaja na Savi i Dunavu, za RAPD analizu korišćeno je mišićno tkivo jedinki Alburnus alburnus (uklija) uzorkovanih sa ukupno devet lokaliteta. RAPD rezultati istraživanja na Savi ukazuju na grupisanje jedinki sa zagađenih lokaliteta (UB i UK), u odnosu na jedinke sa manje zagađenog lokaliteta, SZ. RAPD rezultati studije slučaja na Dunavu ne pokazuju jasno grupisanje uklija u odnosu na jačinu pritisaka što ukazuje na neophodnost dodatnih analiza.
AB  - In recent years the randomly amplified polymorphic DNA method (RAPD) is frequently used in ecogenotoxicology. Within two case studies on the Sava River and the Danube River, muscle tissue of Alburnus alburnus (bleak) specimens sampled from nine sites was used for RAPD analysis. The RAPD results on the Sava River study indicate the grouping of specimens from polluted sites (UB and UK) as opposed to specimens from the less polluted site, SZ. The RAPD results of the Danube River case study do not show a clear bleak grouping in relation to pollution pressures, indicating that additional analyses are needed.
PB  - Belgarde: Serbian Association for Water Protection
C3  - VODA 2021: Zbornik radova 50. godišnje konferencije o aktuelnim  problemima korišćenja i zaštite voda
T1  - Primena RAPD metode u ekogenotoksikološkim istraživanjima – studija slučaja Sava i Dunav
T1  - Application of RAPD method in ecogenotoxicological research research – the Sava River and the Danube River case studies
SP  - 99
EP  - 104
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4735
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Kračun-Kolarević, Margareta and Kolarević, Stoimir and Nikolić, Ivan and Sunjog, Karolina and Paunović, Momir and Vuković-Gačić, Branka",
year = "2021",
abstract = "Metoda nasumično umnožene polimorfne DNK (RAPD) je poslednjih godina našla primenu u ekogenotoksikologiji. U okviru dve studije slučaja na Savi i Dunavu, za RAPD analizu korišćeno je mišićno tkivo jedinki Alburnus alburnus (uklija) uzorkovanih sa ukupno devet lokaliteta. RAPD rezultati istraživanja na Savi ukazuju na grupisanje jedinki sa zagađenih lokaliteta (UB i UK), u odnosu na jedinke sa manje zagađenog lokaliteta, SZ. RAPD rezultati studije slučaja na Dunavu ne pokazuju jasno grupisanje uklija u odnosu na jačinu pritisaka što ukazuje na neophodnost dodatnih analiza., In recent years the randomly amplified polymorphic DNA method (RAPD) is frequently used in ecogenotoxicology. Within two case studies on the Sava River and the Danube River, muscle tissue of Alburnus alburnus (bleak) specimens sampled from nine sites was used for RAPD analysis. The RAPD results on the Sava River study indicate the grouping of specimens from polluted sites (UB and UK) as opposed to specimens from the less polluted site, SZ. The RAPD results of the Danube River case study do not show a clear bleak grouping in relation to pollution pressures, indicating that additional analyses are needed.",
publisher = "Belgarde: Serbian Association for Water Protection",
journal = "VODA 2021: Zbornik radova 50. godišnje konferencije o aktuelnim  problemima korišćenja i zaštite voda",
title = "Primena RAPD metode u ekogenotoksikološkim istraživanjima – studija slučaja Sava i Dunav, Application of RAPD method in ecogenotoxicological research research – the Sava River and the Danube River case studies",
pages = "99-104",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4735"
}

Jovanović Marić, J., Kračun-Kolarević, M., Kolarević, S., Nikolić, I., Sunjog, K., Paunović, M.,& Vuković-Gačić, B.. (2021). Primena RAPD metode u ekogenotoksikološkim istraživanjima – studija slučaja Sava i Dunav. in VODA 2021: Zbornik radova 50. godišnje konferencije o aktuelnim  problemima korišćenja i zaštite voda
Belgarde: Serbian Association for Water Protection., 99-104.
https://hdl.handle.net/21.15107/rcub_ibiss_4735

Jovanović Marić J, Kračun-Kolarević M, Kolarević S, Nikolić I, Sunjog K, Paunović M, Vuković-Gačić B. Primena RAPD metode u ekogenotoksikološkim istraživanjima – studija slučaja Sava i Dunav. in VODA 2021: Zbornik radova 50. godišnje konferencije o aktuelnim  problemima korišćenja i zaštite voda. 2021;:99-104.
https://hdl.handle.net/21.15107/rcub_ibiss_4735 .

Jovanović Marić, Jovana, Kračun-Kolarević, Margareta, Kolarević, Stoimir, Nikolić, Ivan, Sunjog, Karolina, Paunović, Momir, Vuković-Gačić, Branka, "Primena RAPD metode u ekogenotoksikološkim istraživanjima – studija slučaja Sava i Dunav" in VODA 2021: Zbornik radova 50. godišnje konferencije o aktuelnim  problemima korišćenja i zaštite voda (2021):99-104,
https://hdl.handle.net/21.15107/rcub_ibiss_4735 .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Micsinai, Adrienn
AU  - Szántó-Egész, Réka
AU  - Lukács, Alena
AU  - Kračun-Kolarević, Margareta
AU  - Lundy, Lian
AU  - Kirschner, Alexander K.T.
AU  - Farnleitner, Andreas H.
AU  - Đukić, Aleksandar
AU  - Čolić, Jasna
AU  - Nenin, Tanja
AU  - Sunjog, Karolina
AU  - Paunović, Momir
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4214
AB  - In Serbia less than 13% of collected municipal wastewaters is being treated before their release in the environment. This includes all municipal wastewater discharges from Belgrade (capital city of Serbia; population 1,700,000). Previous research has identified the impacts of raw wastewater discharges from Belgrade on the Danube River, and this study investigated if such discharges also provided a pathway for SARS-CoV-2 RNA material. Samples were collected during the most critical circumstances that occurred so far within the COVID-19 pandemics in Serbia. Grab and composite samples were collected in December 2020, during the peak of the third wave (in terms of reported cases) at the site which receives the wastewater loads in Belgrade. Grab samples collected upstream and downstream of Belgrade were also analyzed. RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. SARS-CoV-2 RNA (5.97 × 103 to 1.32 × 104 copies/L) was detected only in samples collected at the site strongly impacted by the wastewaters where all three applied primer sets gave positive signals. Determined concentrations correspond to those reported in wastewater influents sampled at treatment plants in other countries indicating an epidemiological indicator function of used approach for rivers with high pollution loads in countries with poor wastewater treatment.
PB  - Elsevier BV
T2  - Science of The Total Environment
T2  - Science of The Total Environment
T1  - Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters
VL  - 783
DO  - 10.1016/j.scitotenv.2021.146967
SP  - 146967
ER  - 

@article{
author = "Kolarević, Stoimir and Micsinai, Adrienn and Szántó-Egész, Réka and Lukács, Alena and Kračun-Kolarević, Margareta and Lundy, Lian and Kirschner, Alexander K.T. and Farnleitner, Andreas H. and Đukić, Aleksandar and Čolić, Jasna and Nenin, Tanja and Sunjog, Karolina and Paunović, Momir",
year = "2021",
abstract = "In Serbia less than 13% of collected municipal wastewaters is being treated before their release in the environment. This includes all municipal wastewater discharges from Belgrade (capital city of Serbia; population 1,700,000). Previous research has identified the impacts of raw wastewater discharges from Belgrade on the Danube River, and this study investigated if such discharges also provided a pathway for SARS-CoV-2 RNA material. Samples were collected during the most critical circumstances that occurred so far within the COVID-19 pandemics in Serbia. Grab and composite samples were collected in December 2020, during the peak of the third wave (in terms of reported cases) at the site which receives the wastewater loads in Belgrade. Grab samples collected upstream and downstream of Belgrade were also analyzed. RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. SARS-CoV-2 RNA (5.97 × 103 to 1.32 × 104 copies/L) was detected only in samples collected at the site strongly impacted by the wastewaters where all three applied primer sets gave positive signals. Determined concentrations correspond to those reported in wastewater influents sampled at treatment plants in other countries indicating an epidemiological indicator function of used approach for rivers with high pollution loads in countries with poor wastewater treatment.",
publisher = "Elsevier BV",
journal = "Science of The Total Environment, Science of The Total Environment",
title = "Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters",
volume = "783",
doi = "10.1016/j.scitotenv.2021.146967",
pages = "146967"
}

Kolarević, S., Micsinai, A., Szántó-Egész, R., Lukács, A., Kračun-Kolarević, M., Lundy, L., Kirschner, A. K.T., Farnleitner, A. H., Đukić, A., Čolić, J., Nenin, T., Sunjog, K.,& Paunović, M.. (2021). Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters. in Science of The Total Environment
Elsevier BV., 783, 146967.
https://doi.org/10.1016/j.scitotenv.2021.146967

Kolarević S, Micsinai A, Szántó-Egész R, Lukács A, Kračun-Kolarević M, Lundy L, Kirschner AK, Farnleitner AH, Đukić A, Čolić J, Nenin T, Sunjog K, Paunović M. Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters. in Science of The Total Environment. 2021;783:146967.
doi:10.1016/j.scitotenv.2021.146967 .

Kolarević, Stoimir, Micsinai, Adrienn, Szántó-Egész, Réka, Lukács, Alena, Kračun-Kolarević, Margareta, Lundy, Lian, Kirschner, Alexander K.T., Farnleitner, Andreas H., Đukić, Aleksandar, Čolić, Jasna, Nenin, Tanja, Sunjog, Karolina, Paunović, Momir, "Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters" in Science of The Total Environment, 783 (2021):146967,
https://doi.org/10.1016/j.scitotenv.2021.146967 . .

TY  - CHAP
AU  - Kračun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Jovanović, Jovana
AU  - Đorđević, Jelena
AU  - Ilić, Marija
AU  - Sunjog, Karolina
AU  - Kostić-Vuković, Jovana
AU  - Divac Rankov, Aleksandra
AU  - Ilić, Bojan
AU  - Pešić, Vladimir
AU  - Vuković-Gačić, Branka
AU  - Paunović, Momir
PY  - 2020
UR  - https://link.springer.com/chapter/10.1007/698_2019_425
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3951
AB  - Montenegrin surface water and groundwater are important for the Balkan Peninsula since they are connected by the transboundary Dinaric Karst Aquifer System with the waters of additional five countries. The pollution from the surface water can rapidly infiltrate in aquifer and endanger this sensible ecosystem and the health of humans through drinking water supply. This chapter gives insights in the pressures of pollution on Montenegrin waters and in a limited literature data regarding freshwater ecotoxicological studies in Montenegro. Also, this chapter provides new ecotoxicological data obtained during survey in 2019, with a focus on the sites which are identified as hotspots of fecal pollution. The highest responses of biomarkers which indicate embryotoxic, genotoxic, and phytotoxic effects in zebrafish embryo test and in roots of Allium cepa were obtained at Ćehotina – downstream of Pljevlja. Similar results were detected at the site downstream Mojkovac at Tara, yet this site is affected by different type of pollution. Genotoxic endpoints in zebrafish stressed out sites on Morača and Lim rivers which are under pressures of fecal pollution. The data in this chapter provides an insight into current status obtained by the ex situ bioassays and indicates need for more comprehensive in situ assessment.
PB  - Springer
T2  - The Rivers of Montenegro
T1  - Impact of Pollution on Rivers in Montenegro: Ecotoxicological Perspective
DO  - 10.1007/698_2019_425
SP  - 111
EP  - 133
ER  - 

@inbook{
editor = "Pešić, Vladimir, Paunović, Momir, Kostianoy, Andrey G.",
author = "Kračun-Kolarević, Margareta and Kolarević, Stoimir and Jovanović, Jovana and Đorđević, Jelena and Ilić, Marija and Sunjog, Karolina and Kostić-Vuković, Jovana and Divac Rankov, Aleksandra and Ilić, Bojan and Pešić, Vladimir and Vuković-Gačić, Branka and Paunović, Momir",
year = "2020",
abstract = "Montenegrin surface water and groundwater are important for the Balkan Peninsula since they are connected by the transboundary Dinaric Karst Aquifer System with the waters of additional five countries. The pollution from the surface water can rapidly infiltrate in aquifer and endanger this sensible ecosystem and the health of humans through drinking water supply. This chapter gives insights in the pressures of pollution on Montenegrin waters and in a limited literature data regarding freshwater ecotoxicological studies in Montenegro. Also, this chapter provides new ecotoxicological data obtained during survey in 2019, with a focus on the sites which are identified as hotspots of fecal pollution. The highest responses of biomarkers which indicate embryotoxic, genotoxic, and phytotoxic effects in zebrafish embryo test and in roots of Allium cepa were obtained at Ćehotina – downstream of Pljevlja. Similar results were detected at the site downstream Mojkovac at Tara, yet this site is affected by different type of pollution. Genotoxic endpoints in zebrafish stressed out sites on Morača and Lim rivers which are under pressures of fecal pollution. The data in this chapter provides an insight into current status obtained by the ex situ bioassays and indicates need for more comprehensive in situ assessment.",
publisher = "Springer",
journal = "The Rivers of Montenegro",
booktitle = "Impact of Pollution on Rivers in Montenegro: Ecotoxicological Perspective",
doi = "10.1007/698_2019_425",
pages = "111-133"
}

Pešić, V., Paunović, M., Kostianoy, A. G., Kračun-Kolarević, M., Kolarević, S., Jovanović, J., Đorđević, J., Ilić, M., Sunjog, K., Kostić-Vuković, J., Divac Rankov, A., Ilić, B., Pešić, V., Vuković-Gačić, B.,& Paunović, M.. (2020). Impact of Pollution on Rivers in Montenegro: Ecotoxicological Perspective. in The Rivers of Montenegro
Springer., 111-133.
https://doi.org/10.1007/698_2019_425

Pešić V, Paunović M, Kostianoy AG, Kračun-Kolarević M, Kolarević S, Jovanović J, Đorđević J, Ilić M, Sunjog K, Kostić-Vuković J, Divac Rankov A, Ilić B, Pešić V, Vuković-Gačić B, Paunović M. Impact of Pollution on Rivers in Montenegro: Ecotoxicological Perspective. in The Rivers of Montenegro. 2020;:111-133.
doi:10.1007/698_2019_425 .

Pešić, Vladimir, Paunović, Momir, Kostianoy, Andrey G., Kračun-Kolarević, Margareta, Kolarević, Stoimir, Jovanović, Jovana, Đorđević, Jelena, Ilić, Marija, Sunjog, Karolina, Kostić-Vuković, Jovana, Divac Rankov, Aleksandra, Ilić, Bojan, Pešić, Vladimir, Vuković-Gačić, Branka, Paunović, Momir, "Impact of Pollution on Rivers in Montenegro: Ecotoxicological Perspective" in The Rivers of Montenegro (2020):111-133,
https://doi.org/10.1007/698_2019_425 . .

TY  - JOUR
AU  - Radovanović, Jelena
AU  - Antonijević, Biljana
AU  - Kolarević, Stoimir
AU  - Milutinović-Smiljanić, Sanja
AU  - Mandić, Jelena
AU  - Vuković-Gačić, Branka
AU  - Bulat, Zorica
AU  - Ćurčić, Marijana
AU  - Kračun-Kolarević, Margareta
AU  - Sunjog, Karolina
AU  - Kostić-Vuković, Jovana
AU  - Jovanović Marić, Jovana
AU  - Antonijević-Miljaković, Evica
AU  - Đukić-Ćosić, Danijela
AU  - Djordjevic, Aleksandra Buha
AU  - Javorac, Dragana
AU  - Baralić, Katarina
AU  - Mandinić, Zoran
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4065
AB  - The aims of this study were to: (i) examine the toxic effects of sodium fluoride (NaF) in blood, liver, spleen, and brain cells of Wistar rats after the subacute exposure; (ii) explore the potential protective properties of selenium (Se) against fluoride toxicity after the simultaneous administration. Twenty male Wistar rats, eight weeks old, weighing approximately 140–190 g, were divided into four experimental groups (n = 5) as follows: I control-tap water; II NaF 150 ppm; III NaF 150 ppm and Se 1.5 mg/L; IV Se 1.5 mg/L, and had available water with solutions ad libitum for 28 days. DNA damage detected by comet assay was confirmed in the liver, spleen, and brain cells, but not in blood. Selenium supplementation together with NaF decreased DNA damage in liver and spleen cells. According to the histological findings, no changes were observed in spleen and brain tissues after NaF administration. Unlike the observed Se protective effect on the DNA level, no significant reduction of liver tissue injury was observed after the NaF and Se treatment, resulting in mild inflammation. Data of this study suggest that DNA damage after NaF subacute exposure at moderately high concentration was reduced in liver and spleen cells due to Se supplementation, but a similar change was not seen in the brain.
PB  - Elsevier BV
T2  - Chemosphere
T1  - Genotoxicity of Fluoride Subacute Exposure in Rats and Selenium Intervention
DO  - 10.1016/j.chemosphere.2020.128978
SP  - 128978
ER  - 

@article{
author = "Radovanović, Jelena and Antonijević, Biljana and Kolarević, Stoimir and Milutinović-Smiljanić, Sanja and Mandić, Jelena and Vuković-Gačić, Branka and Bulat, Zorica and Ćurčić, Marijana and Kračun-Kolarević, Margareta and Sunjog, Karolina and Kostić-Vuković, Jovana and Jovanović Marić, Jovana and Antonijević-Miljaković, Evica and Đukić-Ćosić, Danijela and Djordjevic, Aleksandra Buha and Javorac, Dragana and Baralić, Katarina and Mandinić, Zoran",
year = "2020",
abstract = "The aims of this study were to: (i) examine the toxic effects of sodium fluoride (NaF) in blood, liver, spleen, and brain cells of Wistar rats after the subacute exposure; (ii) explore the potential protective properties of selenium (Se) against fluoride toxicity after the simultaneous administration. Twenty male Wistar rats, eight weeks old, weighing approximately 140–190 g, were divided into four experimental groups (n = 5) as follows: I control-tap water; II NaF 150 ppm; III NaF 150 ppm and Se 1.5 mg/L; IV Se 1.5 mg/L, and had available water with solutions ad libitum for 28 days. DNA damage detected by comet assay was confirmed in the liver, spleen, and brain cells, but not in blood. Selenium supplementation together with NaF decreased DNA damage in liver and spleen cells. According to the histological findings, no changes were observed in spleen and brain tissues after NaF administration. Unlike the observed Se protective effect on the DNA level, no significant reduction of liver tissue injury was observed after the NaF and Se treatment, resulting in mild inflammation. Data of this study suggest that DNA damage after NaF subacute exposure at moderately high concentration was reduced in liver and spleen cells due to Se supplementation, but a similar change was not seen in the brain.",
publisher = "Elsevier BV",
journal = "Chemosphere",
title = "Genotoxicity of Fluoride Subacute Exposure in Rats and Selenium Intervention",
doi = "10.1016/j.chemosphere.2020.128978",
pages = "128978"
}

Radovanović, J., Antonijević, B., Kolarević, S., Milutinović-Smiljanić, S., Mandić, J., Vuković-Gačić, B., Bulat, Z., Ćurčić, M., Kračun-Kolarević, M., Sunjog, K., Kostić-Vuković, J., Jovanović Marić, J., Antonijević-Miljaković, E., Đukić-Ćosić, D., Djordjevic, A. B., Javorac, D., Baralić, K.,& Mandinić, Z.. (2020). Genotoxicity of Fluoride Subacute Exposure in Rats and Selenium Intervention. in Chemosphere
Elsevier BV., 128978.
https://doi.org/10.1016/j.chemosphere.2020.128978

Radovanović J, Antonijević B, Kolarević S, Milutinović-Smiljanić S, Mandić J, Vuković-Gačić B, Bulat Z, Ćurčić M, Kračun-Kolarević M, Sunjog K, Kostić-Vuković J, Jovanović Marić J, Antonijević-Miljaković E, Đukić-Ćosić D, Djordjevic AB, Javorac D, Baralić K, Mandinić Z. Genotoxicity of Fluoride Subacute Exposure in Rats and Selenium Intervention. in Chemosphere. 2020;:128978.
doi:10.1016/j.chemosphere.2020.128978 .

Radovanović, Jelena, Antonijević, Biljana, Kolarević, Stoimir, Milutinović-Smiljanić, Sanja, Mandić, Jelena, Vuković-Gačić, Branka, Bulat, Zorica, Ćurčić, Marijana, Kračun-Kolarević, Margareta, Sunjog, Karolina, Kostić-Vuković, Jovana, Jovanović Marić, Jovana, Antonijević-Miljaković, Evica, Đukić-Ćosić, Danijela, Djordjevic, Aleksandra Buha, Javorac, Dragana, Baralić, Katarina, Mandinić, Zoran, "Genotoxicity of Fluoride Subacute Exposure in Rats and Selenium Intervention" in Chemosphere (2020):128978,
https://doi.org/10.1016/j.chemosphere.2020.128978 . .

TY  - JOUR
AU  - Jovanović Marić, Jovana
AU  - Kračun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Kostić-Vuković, Jovana
AU  - Deutschmann, Björn
AU  - Hollert, Henner
AU  - Tenji, Dina
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3709
AB  - In this study, few different evaluation concepts were used for the assessment of genotoxic potential at the stretch of the Danube River identified as a significant hotspot of pollution originated through the untreated wastewaters. Three sites were chosen: one site upstream of the wastewater outlet in Novi Sad (Serbia), one at the outlet of wastewaters, and one site few kilometer downstream. Ex situ approach comprised prokaryotic SOS/umuC test on Salmonella typhimurium TA1535/pSK1005 and comet assay on human hepatoma cell line (HepG2). In situ approach was based on the active monitoring (cage approach) using freshwater mussels Sinanodonta woodiana and fish Cyprinus carpio. The comet and micronucleus assays were selected for evaluation of DNA damage in mussel haemocytes and fish blood cells. Within the ex situ part of the study, our results indicated that the eukaryotic model system is more sensitive compared to the prokaryotic one. In situ bioassays are recommended for obtaining a better insight into ecosystem status and in the case of our study the complete insight of genotoxic pressure. However, the choice of animals as bioindicators also has a significant impact on the quality of the obtained information. Differential response between fish and mussels was observed at the highly polluted site suggesting possible involvement of additional protective mechanism such as valve closure in mussels.
PB  - Springer
T2  - Environmental Science and Pollution Research
T1  - Selection of assay, organism, and approach in biomonitoring significantly affects the evaluation of genotoxic potential in aquatic environments
VL  - 27
DO  - 10.1007/s11356-020-09597-0
SP  - 33903
EP  - 33915
ER  - 

@article{
author = "Jovanović Marić, Jovana and Kračun-Kolarević, Margareta and Kolarević, Stoimir and Sunjog, Karolina and Kostić-Vuković, Jovana and Deutschmann, Björn and Hollert, Henner and Tenji, Dina and Paunović, Momir and Vuković-Gačić, Branka",
year = "2020",
abstract = "In this study, few different evaluation concepts were used for the assessment of genotoxic potential at the stretch of the Danube River identified as a significant hotspot of pollution originated through the untreated wastewaters. Three sites were chosen: one site upstream of the wastewater outlet in Novi Sad (Serbia), one at the outlet of wastewaters, and one site few kilometer downstream. Ex situ approach comprised prokaryotic SOS/umuC test on Salmonella typhimurium TA1535/pSK1005 and comet assay on human hepatoma cell line (HepG2). In situ approach was based on the active monitoring (cage approach) using freshwater mussels Sinanodonta woodiana and fish Cyprinus carpio. The comet and micronucleus assays were selected for evaluation of DNA damage in mussel haemocytes and fish blood cells. Within the ex situ part of the study, our results indicated that the eukaryotic model system is more sensitive compared to the prokaryotic one. In situ bioassays are recommended for obtaining a better insight into ecosystem status and in the case of our study the complete insight of genotoxic pressure. However, the choice of animals as bioindicators also has a significant impact on the quality of the obtained information. Differential response between fish and mussels was observed at the highly polluted site suggesting possible involvement of additional protective mechanism such as valve closure in mussels.",
publisher = "Springer",
journal = "Environmental Science and Pollution Research",
title = "Selection of assay, organism, and approach in biomonitoring significantly affects the evaluation of genotoxic potential in aquatic environments",
volume = "27",
doi = "10.1007/s11356-020-09597-0",
pages = "33903-33915"
}

Jovanović Marić, J., Kračun-Kolarević, M., Kolarević, S., Sunjog, K., Kostić-Vuković, J., Deutschmann, B., Hollert, H., Tenji, D., Paunović, M.,& Vuković-Gačić, B.. (2020). Selection of assay, organism, and approach in biomonitoring significantly affects the evaluation of genotoxic potential in aquatic environments. in Environmental Science and Pollution Research
Springer., 27, 33903-33915.
https://doi.org/10.1007/s11356-020-09597-0

Jovanović Marić J, Kračun-Kolarević M, Kolarević S, Sunjog K, Kostić-Vuković J, Deutschmann B, Hollert H, Tenji D, Paunović M, Vuković-Gačić B. Selection of assay, organism, and approach in biomonitoring significantly affects the evaluation of genotoxic potential in aquatic environments. in Environmental Science and Pollution Research. 2020;27:33903-33915.
doi:10.1007/s11356-020-09597-0 .

Jovanović Marić, Jovana, Kračun-Kolarević, Margareta, Kolarević, Stoimir, Sunjog, Karolina, Kostić-Vuković, Jovana, Deutschmann, Björn, Hollert, Henner, Tenji, Dina, Paunović, Momir, Vuković-Gačić, Branka, "Selection of assay, organism, and approach in biomonitoring significantly affects the evaluation of genotoxic potential in aquatic environments" in Environmental Science and Pollution Research, 27 (2020):33903-33915,
https://doi.org/10.1007/s11356-020-09597-0 . .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Kračun-Kolarević, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Jovanović, Jovana
AU  - Simonović, Predrag
AU  - Simić, Vladica
AU  - Piria, Marina
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2018
UR  - http://link.springer.com/10.1007/s41742-018-0127-6
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3132
AB  - The major objective of this study was to investigate the impact of wastewaters on the level of genotoxicity and the possible reflection of genotoxicity on the genetic variability in chub (Squalius cephalus) collected from the sites under different pollution pressures along the Sava River. To emphasize the impact of wastewaters, this study was carried out in a season with low water discharge. The level of genotoxic potential at the sites was studied by alkaline and Fpg-modified comet assay in fish blood, while RAPD technique was employed on the fish muscle tissue. The variations in the genotoxic potential were observed along the Sava River based on the level of DNA damage in blood of the specimens collected at the selected sites. Clustering of the impacted sites was also observed based on RAPD fingerprints. Comparison of the data obtained in RAPD and the data from the comet assay indicated the possible reflection of genotoxicity on the genetic variability in chub collected from the sites with different pollution pressure. This is a preliminary study which reports the differentiation of the population of chub along the Sava River based on RAPD analysis.
T2  - International Journal of Environmental Research
T1  - The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study
IS  - 5
VL  - 12
DO  - 10.1007/s41742-018-0127-6
SP  - 703
EP  - 712
ER  - 

@article{
author = "Kolarević, Stoimir and Sunjog, Karolina and Kračun-Kolarević, Margareta and Kostić-Vuković, Jovana and Jovanović, Jovana and Simonović, Predrag and Simić, Vladica and Piria, Marina and Gačić, Zoran and Lenhardt, Mirjana and Paunović, Momir and Vuković-Gačić, Branka",
year = "2018",
abstract = "The major objective of this study was to investigate the impact of wastewaters on the level of genotoxicity and the possible reflection of genotoxicity on the genetic variability in chub (Squalius cephalus) collected from the sites under different pollution pressures along the Sava River. To emphasize the impact of wastewaters, this study was carried out in a season with low water discharge. The level of genotoxic potential at the sites was studied by alkaline and Fpg-modified comet assay in fish blood, while RAPD technique was employed on the fish muscle tissue. The variations in the genotoxic potential were observed along the Sava River based on the level of DNA damage in blood of the specimens collected at the selected sites. Clustering of the impacted sites was also observed based on RAPD fingerprints. Comparison of the data obtained in RAPD and the data from the comet assay indicated the possible reflection of genotoxicity on the genetic variability in chub collected from the sites with different pollution pressure. This is a preliminary study which reports the differentiation of the population of chub along the Sava River based on RAPD analysis.",
journal = "International Journal of Environmental Research",
title = "The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study",
number = "5",
volume = "12",
doi = "10.1007/s41742-018-0127-6",
pages = "703-712"
}

Kolarević, S., Sunjog, K., Kračun-Kolarević, M., Kostić-Vuković, J., Jovanović, J., Simonović, P., Simić, V., Piria, M., Gačić, Z., Lenhardt, M., Paunović, M.,& Vuković-Gačić, B.. (2018). The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study. in International Journal of Environmental Research, 12(5), 703-712.
https://doi.org/10.1007/s41742-018-0127-6

Kolarević S, Sunjog K, Kračun-Kolarević M, Kostić-Vuković J, Jovanović J, Simonović P, Simić V, Piria M, Gačić Z, Lenhardt M, Paunović M, Vuković-Gačić B. The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study. in International Journal of Environmental Research. 2018;12(5):703-712.
doi:10.1007/s41742-018-0127-6 .

Kolarević, Stoimir, Sunjog, Karolina, Kračun-Kolarević, Margareta, Kostić-Vuković, Jovana, Jovanović, Jovana, Simonović, Predrag, Simić, Vladica, Piria, Marina, Gačić, Zoran, Lenhardt, Mirjana, Paunović, Momir, Vuković-Gačić, Branka, "The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study" in International Journal of Environmental Research, 12, no. 5 (2018):703-712,
https://doi.org/10.1007/s41742-018-0127-6 . .

TY  - CHAP
AU  - Kapetanović, Damir
AU  - Vardić Smrzlić, Irena
AU  - Valić, Damir
AU  - Teskeredžić, Emin
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Tomović, Jelena
AU  - Kračun-Kolarević, Margareta
AU  - Knežević-Vukčević, Jelena
AU  - Paunović, Momir
AU  - Gačić, Zoran
AU  - Vuković-Gačić, Branka
PY  - 2015
UR  - http://link.springer.com/chapter/10.1007/978-3-662-44034-6_9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2569
AB  - Data on the microbiological quality of the freshwater systems under the anthropogenic influence, such as the Sava River, are of the major importance for the water resource management. Furthermore, analyses of the microbial quality of fish meat provide information of the fish as a valuable food resource from the investigated river basin. The health status of the fish, including dynamics of infection and biodiversity of endoparasites, is important bioindicator of changes in the ecosystem structure and function. For the ecosystem-based approach to the Sava River management, investigations of microbiological quality of the Sava River water and the meat of the European chub as the bioindicator organism, as well as dynamics of infection/biodiversity of intestinal parasites Acanthocephala, were performed. The survey comprised the data collected in periods 2005, 2006 and 2012. Microbiological investigation of water was performed in 2006 and 2012, while microbiological analyses of fish meat and ichthyo-parasitological investigation took place during 2005–2006. A high number of heterotrophic bacteria were recorded during 2006 survey, confirmed by the distinctly higher values of the three faecal indicators (total coliform, E. coli and enterococci), and indicated poor water quality downstream of the cities Zagreb and Velika Gorica, as a result of the municipal sewage outlets. The results from 2012 survey indicated the existence of moderate to critical faecal and organic pollution in all samples. Accumulation of the bacteria in the European chub meat was mainly uniform along the watercourse within standards and limitations for the human consumption. Sampling sites downstream cities of Zagreb and Velika Gorica were characterised with the lower prevalence and abundance of two common species of the chub intestinal acanthocephalan parasites, Pomphorhynchus laevis and Acanthocephalus anguillae. Poor microbiological quality of the water and lower distribution of chub intestinal parasites were related to the anthropogenic influence, downstream of the urban areas.
PB  - Springer-Verlag, Berlin, Heidelberg
T2  - The Sava River
T1  - Microbial Characterisation of the Sava River
DO  - 10.1007/978-3-662-44034-6_9
SP  - 201
EP  - 228
ER  - 

@inbook{
editor = "Milačić, Radmila, Ščančar, Janez, Paunović, Momir",
author = "Kapetanović, Damir and Vardić Smrzlić, Irena and Valić, Damir and Teskeredžić, Emin and Kolarević, Stoimir and Sunjog, Karolina and Tomović, Jelena and Kračun-Kolarević, Margareta and Knežević-Vukčević, Jelena and Paunović, Momir and Gačić, Zoran and Vuković-Gačić, Branka",
year = "2015",
abstract = "Data on the microbiological quality of the freshwater systems under the anthropogenic influence, such as the Sava River, are of the major importance for the water resource management. Furthermore, analyses of the microbial quality of fish meat provide information of the fish as a valuable food resource from the investigated river basin. The health status of the fish, including dynamics of infection and biodiversity of endoparasites, is important bioindicator of changes in the ecosystem structure and function. For the ecosystem-based approach to the Sava River management, investigations of microbiological quality of the Sava River water and the meat of the European chub as the bioindicator organism, as well as dynamics of infection/biodiversity of intestinal parasites Acanthocephala, were performed. The survey comprised the data collected in periods 2005, 2006 and 2012. Microbiological investigation of water was performed in 2006 and 2012, while microbiological analyses of fish meat and ichthyo-parasitological investigation took place during 2005–2006. A high number of heterotrophic bacteria were recorded during 2006 survey, confirmed by the distinctly higher values of the three faecal indicators (total coliform, E. coli and enterococci), and indicated poor water quality downstream of the cities Zagreb and Velika Gorica, as a result of the municipal sewage outlets. The results from 2012 survey indicated the existence of moderate to critical faecal and organic pollution in all samples. Accumulation of the bacteria in the European chub meat was mainly uniform along the watercourse within standards and limitations for the human consumption. Sampling sites downstream cities of Zagreb and Velika Gorica were characterised with the lower prevalence and abundance of two common species of the chub intestinal acanthocephalan parasites, Pomphorhynchus laevis and Acanthocephalus anguillae. Poor microbiological quality of the water and lower distribution of chub intestinal parasites were related to the anthropogenic influence, downstream of the urban areas.",
publisher = "Springer-Verlag, Berlin, Heidelberg",
journal = "The Sava River",
booktitle = "Microbial Characterisation of the Sava River",
doi = "10.1007/978-3-662-44034-6_9",
pages = "201-228"
}

Milačić, R., Ščančar, J., Paunović, M., Kapetanović, D., Vardić Smrzlić, I., Valić, D., Teskeredžić, E., Kolarević, S., Sunjog, K., Tomović, J., Kračun-Kolarević, M., Knežević-Vukčević, J., Paunović, M., Gačić, Z.,& Vuković-Gačić, B.. (2015). Microbial Characterisation of the Sava River. in The Sava River
Springer-Verlag, Berlin, Heidelberg., 201-228.
https://doi.org/10.1007/978-3-662-44034-6_9

Milačić R, Ščančar J, Paunović M, Kapetanović D, Vardić Smrzlić I, Valić D, Teskeredžić E, Kolarević S, Sunjog K, Tomović J, Kračun-Kolarević M, Knežević-Vukčević J, Paunović M, Gačić Z, Vuković-Gačić B. Microbial Characterisation of the Sava River. in The Sava River. 2015;:201-228.
doi:10.1007/978-3-662-44034-6_9 .

Milačić, Radmila, Ščančar, Janez, Paunović, Momir, Kapetanović, Damir, Vardić Smrzlić, Irena, Valić, Damir, Teskeredžić, Emin, Kolarević, Stoimir, Sunjog, Karolina, Tomović, Jelena, Kračun-Kolarević, Margareta, Knežević-Vukčević, Jelena, Paunović, Momir, Gačić, Zoran, Vuković-Gačić, Branka, "Microbial Characterisation of the Sava River" in The Sava River (2015):201-228,
https://doi.org/10.1007/978-3-662-44034-6_9 . .