TY  - JOUR
AU  - Matić, Dragana
AU  - Vlahović, Milena
AU  - Grčić, Anja
AU  - Filipović, Aleksandra
AU  - Ilijin, Larisa
AU  - Mrdaković, Marija
AU  - Mutić, Jelena
AU  - Đurđić, Slađana
AU  - Perić Mataruga, Vesna
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6079
AB  - Long-term exposure of populations to pollution may result in enhanced ability to cope with environmental stress. To compare the responses of two Lymantria dispar populations living in unpolluted and polluted forests (UP and PP, respectively), we chronically exposed larvae to cadmium at concentrations of 50 and 100 μg Cd/g dry food (Cd1 and Cd2, respectively). We examined cadmium accumulation in the midgut and hemolymph, activities of superoxide dismutase (SOD), catalase (CAT), and alkaline phosphatases (ALP) in the midgut, as well as Hsp70 protein expression in the midgut, hemolymph, and brain and evaluated these parameters as biomarkers of cadmium contamination. Larvae from PP, fed a control diet, showed higher activity of SOD and increased Hsp70 expression compared with larvae from UP. Excessive amounts of Cd were accumulated in the midgut of all Cd-fed larvae, whereas Cd content in the hemolymph was elevated only in larvae from PP after Cd2 treatment. In larvae from UP, Cd2 treatment decreased the activity of CAT and induced the expression of Hsp70 in the midgut and hemolymph. In larvae from PP, exposure to both Cd concentrations strongly attenuated SOD and CAT activities, while Hsp70 expression was not induced in any organ/tissue. Cd did not affect ALP activity in either population. Midgut Cd content proved to be a suitable indicator of Cd contamination for both polluted and unpolluted habitats.
PB  - Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Antioxidative enzymes, alkaline phosphatases and Hsp70 expression in larvae of Lymantria dispar (Lepidoptera: Erebidae) from unpolluted and polluted forests after chronic cadmium treatment
VL  - 273
DO  - 10.1016/j.cbpc.2023.109721
SP  - 109721
ER  - 

@article{
author = "Matić, Dragana and Vlahović, Milena and Grčić, Anja and Filipović, Aleksandra and Ilijin, Larisa and Mrdaković, Marija and Mutić, Jelena and Đurđić, Slađana and Perić Mataruga, Vesna",
year = "2023",
abstract = "Long-term exposure of populations to pollution may result in enhanced ability to cope with environmental stress. To compare the responses of two Lymantria dispar populations living in unpolluted and polluted forests (UP and PP, respectively), we chronically exposed larvae to cadmium at concentrations of 50 and 100 μg Cd/g dry food (Cd1 and Cd2, respectively). We examined cadmium accumulation in the midgut and hemolymph, activities of superoxide dismutase (SOD), catalase (CAT), and alkaline phosphatases (ALP) in the midgut, as well as Hsp70 protein expression in the midgut, hemolymph, and brain and evaluated these parameters as biomarkers of cadmium contamination. Larvae from PP, fed a control diet, showed higher activity of SOD and increased Hsp70 expression compared with larvae from UP. Excessive amounts of Cd were accumulated in the midgut of all Cd-fed larvae, whereas Cd content in the hemolymph was elevated only in larvae from PP after Cd2 treatment. In larvae from UP, Cd2 treatment decreased the activity of CAT and induced the expression of Hsp70 in the midgut and hemolymph. In larvae from PP, exposure to both Cd concentrations strongly attenuated SOD and CAT activities, while Hsp70 expression was not induced in any organ/tissue. Cd did not affect ALP activity in either population. Midgut Cd content proved to be a suitable indicator of Cd contamination for both polluted and unpolluted habitats.",
publisher = "Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Antioxidative enzymes, alkaline phosphatases and Hsp70 expression in larvae of Lymantria dispar (Lepidoptera: Erebidae) from unpolluted and polluted forests after chronic cadmium treatment",
volume = "273",
doi = "10.1016/j.cbpc.2023.109721",
pages = "109721"
}

Matić, D., Vlahović, M., Grčić, A., Filipović, A., Ilijin, L., Mrdaković, M., Mutić, J., Đurđić, S.,& Perić Mataruga, V.. (2023). Antioxidative enzymes, alkaline phosphatases and Hsp70 expression in larvae of Lymantria dispar (Lepidoptera: Erebidae) from unpolluted and polluted forests after chronic cadmium treatment. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
Elsevier Inc.., 273, 109721.
https://doi.org/10.1016/j.cbpc.2023.109721

Matić D, Vlahović M, Grčić A, Filipović A, Ilijin L, Mrdaković M, Mutić J, Đurđić S, Perić Mataruga V. Antioxidative enzymes, alkaline phosphatases and Hsp70 expression in larvae of Lymantria dispar (Lepidoptera: Erebidae) from unpolluted and polluted forests after chronic cadmium treatment. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2023;273:109721.
doi:10.1016/j.cbpc.2023.109721 .

Matić, Dragana, Vlahović, Milena, Grčić, Anja, Filipović, Aleksandra, Ilijin, Larisa, Mrdaković, Marija, Mutić, Jelena, Đurđić, Slađana, Perić Mataruga, Vesna, "Antioxidative enzymes, alkaline phosphatases and Hsp70 expression in larvae of Lymantria dispar (Lepidoptera: Erebidae) from unpolluted and polluted forests after chronic cadmium treatment" in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 273 (2023):109721,
https://doi.org/10.1016/j.cbpc.2023.109721 . .

TY  - JOUR
AU  - Grčić, Anja
AU  - Ilijin, Larisa
AU  - Filipović, Aleksandra
AU  - Matić, Dragana
AU  - Mrdaković, Marija
AU  - Todorović, Dajana
AU  - Vlahović, Milena
AU  - Perić Mataruga, Vesna
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6074
AB  - Global, unpredictable temperature increases have strong effects on all organisms, especially insects. Elucidating the effects of short-term temperature increases on midgut digestive enzymes (α-glucosidase, lipase, trypsin, and leucine aminopeptidase - LAP) and metabolic macromolecules in the hemolymph (proteins, lipids, and trehalose) of phytophagous pest larvae of Lymantria dispar is important for general considerations of insect adaptation to a warming climate and potential pest control options. We also wanted to determine whether the different adaptations of L. dispar populations to environmental pollution might affect their ability to cope with heat stress using larvae from the undisturbed, Kosmaj forest and disturbed, Lipovica forest. Heat treatments at 28 °C increased α-glucosidase activity in both larval populations, inhibited LAP activity in larvae from the polluted forest, and had no significant effect on trypsin and lipase activities, regardless of larval origin. The concentration of proteins, lipids, and trehalose in the hemolymph of larvae from the disturbed forest increased, whereas the population from the undisturbed forest showed only an increase in proteins and lipids after the heat treatments. Larval mass was also increased in larvae from the undisturbed forest. Our results suggest a higher sensitivity of digestive enzymes and metabolism to short-term heat stress in L. dispar populations adapted to pollution in their forest habitat, although climate warming is not beneficial even for populations from unpolluted forests. The digestive and metabolic processes of L. dispar larvae are substantially affected by sublethal short-term increases in ambient temperature.
PB  - San Diego: Academic Press Inc.
T2  - Environmental Research
T1  - Digestive enzyme activity and macromolecule content in the hemolymph of differentially adapted Lymantria dispar L. populations after short-term increases in ambient temperature
IS  - 116461
IS  - Part 1
VL  - 236
DO  - 10.1016/j.envres.2023.116461
ER  - 

@article{
author = "Grčić, Anja and Ilijin, Larisa and Filipović, Aleksandra and Matić, Dragana and Mrdaković, Marija and Todorović, Dajana and Vlahović, Milena and Perić Mataruga, Vesna",
year = "2023",
abstract = "Global, unpredictable temperature increases have strong effects on all organisms, especially insects. Elucidating the effects of short-term temperature increases on midgut digestive enzymes (α-glucosidase, lipase, trypsin, and leucine aminopeptidase - LAP) and metabolic macromolecules in the hemolymph (proteins, lipids, and trehalose) of phytophagous pest larvae of Lymantria dispar is important for general considerations of insect adaptation to a warming climate and potential pest control options. We also wanted to determine whether the different adaptations of L. dispar populations to environmental pollution might affect their ability to cope with heat stress using larvae from the undisturbed, Kosmaj forest and disturbed, Lipovica forest. Heat treatments at 28 °C increased α-glucosidase activity in both larval populations, inhibited LAP activity in larvae from the polluted forest, and had no significant effect on trypsin and lipase activities, regardless of larval origin. The concentration of proteins, lipids, and trehalose in the hemolymph of larvae from the disturbed forest increased, whereas the population from the undisturbed forest showed only an increase in proteins and lipids after the heat treatments. Larval mass was also increased in larvae from the undisturbed forest. Our results suggest a higher sensitivity of digestive enzymes and metabolism to short-term heat stress in L. dispar populations adapted to pollution in their forest habitat, although climate warming is not beneficial even for populations from unpolluted forests. The digestive and metabolic processes of L. dispar larvae are substantially affected by sublethal short-term increases in ambient temperature.",
publisher = "San Diego: Academic Press Inc.",
journal = "Environmental Research",
title = "Digestive enzyme activity and macromolecule content in the hemolymph of differentially adapted Lymantria dispar L. populations after short-term increases in ambient temperature",
number = "116461, Part 1",
volume = "236",
doi = "10.1016/j.envres.2023.116461"
}

Grčić, A., Ilijin, L., Filipović, A., Matić, D., Mrdaković, M., Todorović, D., Vlahović, M.,& Perić Mataruga, V.. (2023). Digestive enzyme activity and macromolecule content in the hemolymph of differentially adapted Lymantria dispar L. populations after short-term increases in ambient temperature. in Environmental Research
San Diego: Academic Press Inc.., 236(116461).
https://doi.org/10.1016/j.envres.2023.116461

Grčić A, Ilijin L, Filipović A, Matić D, Mrdaković M, Todorović D, Vlahović M, Perić Mataruga V. Digestive enzyme activity and macromolecule content in the hemolymph of differentially adapted Lymantria dispar L. populations after short-term increases in ambient temperature. in Environmental Research. 2023;236(116461).
doi:10.1016/j.envres.2023.116461 .

Grčić, Anja, Ilijin, Larisa, Filipović, Aleksandra, Matić, Dragana, Mrdaković, Marija, Todorović, Dajana, Vlahović, Milena, Perić Mataruga, Vesna, "Digestive enzyme activity and macromolecule content in the hemolymph of differentially adapted Lymantria dispar L. populations after short-term increases in ambient temperature" in Environmental Research, 236, no. 116461 (2023),
https://doi.org/10.1016/j.envres.2023.116461 . .

TY  - JOUR
AU  - Mrdaković, Marija
AU  - Filipović, Aleksandra
AU  - Ilijin, Larisa
AU  - Grčić, Anja
AU  - Matić, Dragana
AU  - Vlahović, Milena
AU  - Todorović, Dajana
AU  - Perić Mataruga, Vesna
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5753
AB  - In this study, responses of carboxylesterases, acetylcholinesterase, and stress protein Hsp70 were examined in the midgut and midgut tissue, and brain of fifth instar larvae of Lymantria dispar L. and Euproctis chrysorrhoea L. following chronic exposure to dietary fluoranthene. Specific carboxylesterase activity increased significantly in the midgut tissue of E. chrysorrhoea larvae treated with a lower fluoranthene concentration. The specific patterns of isoforms expression, recorded in larvae of both species, enable efficient carboxylesterase activity as a significant part of defense mechanisms. Increased Hsp70 concentration in the brain of L. dispar larvae points to a response to the proteotoxic effects of a lower fluoranthene concentration. Decreased Hsp70 in the brain of E. chrysorrhoea larvae in both treated groups can suggest induction of other mechanisms of defense. The results indicate the importance of the examined parameters in larvae of both species exposed to the pollutant, as well as
their potential as biomarkers.
PB  - Academic Press
T2  - Ecotoxicology and Environmental Safety
T1  - Effects of dietary fluoranthene on tissue-specific responses of carboxylesterases, acetylcholinesterase and heat shock protein 70 in two forest lepidopteran species
VL  - 257
DO  - 10.1016/j.ecoenv.2023.114937
SP  - 114937
ER  - 

@article{
author = "Mrdaković, Marija and Filipović, Aleksandra and Ilijin, Larisa and Grčić, Anja and Matić, Dragana and Vlahović, Milena and Todorović, Dajana and Perić Mataruga, Vesna",
year = "2023",
abstract = "In this study, responses of carboxylesterases, acetylcholinesterase, and stress protein Hsp70 were examined in the midgut and midgut tissue, and brain of fifth instar larvae of Lymantria dispar L. and Euproctis chrysorrhoea L. following chronic exposure to dietary fluoranthene. Specific carboxylesterase activity increased significantly in the midgut tissue of E. chrysorrhoea larvae treated with a lower fluoranthene concentration. The specific patterns of isoforms expression, recorded in larvae of both species, enable efficient carboxylesterase activity as a significant part of defense mechanisms. Increased Hsp70 concentration in the brain of L. dispar larvae points to a response to the proteotoxic effects of a lower fluoranthene concentration. Decreased Hsp70 in the brain of E. chrysorrhoea larvae in both treated groups can suggest induction of other mechanisms of defense. The results indicate the importance of the examined parameters in larvae of both species exposed to the pollutant, as well as
their potential as biomarkers.",
publisher = "Academic Press",
journal = "Ecotoxicology and Environmental Safety",
title = "Effects of dietary fluoranthene on tissue-specific responses of carboxylesterases, acetylcholinesterase and heat shock protein 70 in two forest lepidopteran species",
volume = "257",
doi = "10.1016/j.ecoenv.2023.114937",
pages = "114937"
}

Mrdaković, M., Filipović, A., Ilijin, L., Grčić, A., Matić, D., Vlahović, M., Todorović, D.,& Perić Mataruga, V.. (2023). Effects of dietary fluoranthene on tissue-specific responses of carboxylesterases, acetylcholinesterase and heat shock protein 70 in two forest lepidopteran species. in Ecotoxicology and Environmental Safety
Academic Press., 257, 114937.
https://doi.org/10.1016/j.ecoenv.2023.114937

Mrdaković M, Filipović A, Ilijin L, Grčić A, Matić D, Vlahović M, Todorović D, Perić Mataruga V. Effects of dietary fluoranthene on tissue-specific responses of carboxylesterases, acetylcholinesterase and heat shock protein 70 in two forest lepidopteran species. in Ecotoxicology and Environmental Safety. 2023;257:114937.
doi:10.1016/j.ecoenv.2023.114937 .

Mrdaković, Marija, Filipović, Aleksandra, Ilijin, Larisa, Grčić, Anja, Matić, Dragana, Vlahović, Milena, Todorović, Dajana, Perić Mataruga, Vesna, "Effects of dietary fluoranthene on tissue-specific responses of carboxylesterases, acetylcholinesterase and heat shock protein 70 in two forest lepidopteran species" in Ecotoxicology and Environmental Safety, 257 (2023):114937,
https://doi.org/10.1016/j.ecoenv.2023.114937 . .

TY  - JOUR
AU  - Ilijin, Larisa
AU  - Grčić, Anja
AU  - Mrdaković, Marija
AU  - Vlahović, Milena
AU  - Todorović, Dajana
AU  - Filipović, Aleksandra
AU  - Matić, Dragana
AU  - Perić Mataruga, Vesna
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5308
AB  - Increased environmental temperature is one of the most frequent stresses effecting metabolic rate in herbivorous insect species. Our goal was to compare the influence of increased environmental temperature and induced thermotolerance on the activity of midgut phosphatases and brain tissue hsp70 concentration in 5th instar Lymantria dispar larvae originating from an unpolluted and polluted forest. Induced thermotolerance (larval pre-treatment at high, sub-lethal temperature) increases the species ability to overcome the negative effects of thermal stress, therefore we monitored the effect of this regime in larvae originating from both forests. Thermal regimes in this experiment predominantly influenced the alkaline phosphatases  activity and it was affected by temperature, population origin, and their combined effect. Total acid phosphatases activity was changed only by the joint effect of temperature and population origin. Brain hsp70 concentration was under a significant individual and joint effect of temperature and population. In both populations, brain tissue hsp70 concentration and alkaline phosphatases activity should be taken under consideration as a battery with biomarker potential for thermal stress in L. dispar larvae as a bioindicator species.
PB  - London: Nature Publishing Group
T2  - Scientific Reports
T1  - The effects of temperature stress and population origin on the thermal sensitivity of Lymantria dispar L. (Lepidoptera: Erebidae) larvae
IS  - 12
DO  - 10.1038/s41598-022-26506-2
SP  - 21858
ER  - 

@article{
author = "Ilijin, Larisa and Grčić, Anja and Mrdaković, Marija and Vlahović, Milena and Todorović, Dajana and Filipović, Aleksandra and Matić, Dragana and Perić Mataruga, Vesna",
year = "2022",
abstract = "Increased environmental temperature is one of the most frequent stresses effecting metabolic rate in herbivorous insect species. Our goal was to compare the influence of increased environmental temperature and induced thermotolerance on the activity of midgut phosphatases and brain tissue hsp70 concentration in 5th instar Lymantria dispar larvae originating from an unpolluted and polluted forest. Induced thermotolerance (larval pre-treatment at high, sub-lethal temperature) increases the species ability to overcome the negative effects of thermal stress, therefore we monitored the effect of this regime in larvae originating from both forests. Thermal regimes in this experiment predominantly influenced the alkaline phosphatases  activity and it was affected by temperature, population origin, and their combined effect. Total acid phosphatases activity was changed only by the joint effect of temperature and population origin. Brain hsp70 concentration was under a significant individual and joint effect of temperature and population. In both populations, brain tissue hsp70 concentration and alkaline phosphatases activity should be taken under consideration as a battery with biomarker potential for thermal stress in L. dispar larvae as a bioindicator species.",
publisher = "London: Nature Publishing Group",
journal = "Scientific Reports",
title = "The effects of temperature stress and population origin on the thermal sensitivity of Lymantria dispar L. (Lepidoptera: Erebidae) larvae",
number = "12",
doi = "10.1038/s41598-022-26506-2",
pages = "21858"
}

Ilijin, L., Grčić, A., Mrdaković, M., Vlahović, M., Todorović, D., Filipović, A., Matić, D.,& Perić Mataruga, V.. (2022). The effects of temperature stress and population origin on the thermal sensitivity of Lymantria dispar L. (Lepidoptera: Erebidae) larvae. in Scientific Reports
London: Nature Publishing Group.(12), 21858.
https://doi.org/10.1038/s41598-022-26506-2

Ilijin L, Grčić A, Mrdaković M, Vlahović M, Todorović D, Filipović A, Matić D, Perić Mataruga V. The effects of temperature stress and population origin on the thermal sensitivity of Lymantria dispar L. (Lepidoptera: Erebidae) larvae. in Scientific Reports. 2022;(12):21858.
doi:10.1038/s41598-022-26506-2 .

Ilijin, Larisa, Grčić, Anja, Mrdaković, Marija, Vlahović, Milena, Todorović, Dajana, Filipović, Aleksandra, Matić, Dragana, Perić Mataruga, Vesna, "The effects of temperature stress and population origin on the thermal sensitivity of Lymantria dispar L. (Lepidoptera: Erebidae) larvae" in Scientific Reports, no. 12 (2022):21858,
https://doi.org/10.1038/s41598-022-26506-2 . .

TY  - JOUR
AU  - Vlahović, Milena
AU  - Matić, Dragana
AU  - Mrdaković, Marija
AU  - Ilijin, Larisa
AU  - Grčić, Anja
AU  - Filipović, Aleksandra
AU  - Lazarević, Jelica
AU  - Perić Mataruga, Vesna
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5361
AB  - Cadmium pollution is becoming an increasing problem, especially in parts of the world that have developed industries. To consider the potentially harmful effects of cadmium, we need to examine changes at all different levels of biological organization. The main goal of this study was to detect a possible change in the percentage of midgut mass relative to larval mass (PMM) and determine the plasticity of this trait and the correlations between midgut enzymes and PMM under stress conditions. Fourth-instar larvae were exposed to acute and chronic effects of two cadmium concentrations, 10 and 30 μg Cd/g dry food, as well as a three-day recovery from chronic treatments. PMM is also an indirect indicator of food consumption and was found to be significantly reduced compared to control in both acute effects and chronic treatment at 30 μg and its three-day recovery. The PMM reduction during acute treatments is a consequence of cadmium action, while in chronic treatment, the genetic factor (egg mass) plays a crucial role in the change of PMM. According to the index of plasticity, distinct phenotypes were not produced. Significant correlations were shown between PMM and trypsin (Tryp) and leucine aminopeptidases (LAP) at acute and chronic treatment with higher cadmium concentrations, while significant correlations between proteases and PMM were detected at lower metal concentrations (Acute10 and Chronic10 and 30 μg Cd/g dry food). In contrast to chronic treatment, egg masses respond more uniformly by reducing PMM during the short-term effect of cadmium. Finally, we can conclude that, as an addition to biochemical and molecular research, PMM can be used for studying the cadmium effects to gain a better insight into the state of the organism under stress conditions.
PB  - Lahore: Zoological Society of Pakistan
T2  - Pakistan Journal of Zoology
T1  - The Influence of Dietary Cadmium on Changes in the Midgut Mass Related to the Mass of Gypsy Moth Larvae
DO  - 10.17582/journal.pjz/20211019131007
ER  - 

@article{
author = "Vlahović, Milena and Matić, Dragana and Mrdaković, Marija and Ilijin, Larisa and Grčić, Anja and Filipović, Aleksandra and Lazarević, Jelica and Perić Mataruga, Vesna",
year = "2022",
abstract = "Cadmium pollution is becoming an increasing problem, especially in parts of the world that have developed industries. To consider the potentially harmful effects of cadmium, we need to examine changes at all different levels of biological organization. The main goal of this study was to detect a possible change in the percentage of midgut mass relative to larval mass (PMM) and determine the plasticity of this trait and the correlations between midgut enzymes and PMM under stress conditions. Fourth-instar larvae were exposed to acute and chronic effects of two cadmium concentrations, 10 and 30 μg Cd/g dry food, as well as a three-day recovery from chronic treatments. PMM is also an indirect indicator of food consumption and was found to be significantly reduced compared to control in both acute effects and chronic treatment at 30 μg and its three-day recovery. The PMM reduction during acute treatments is a consequence of cadmium action, while in chronic treatment, the genetic factor (egg mass) plays a crucial role in the change of PMM. According to the index of plasticity, distinct phenotypes were not produced. Significant correlations were shown between PMM and trypsin (Tryp) and leucine aminopeptidases (LAP) at acute and chronic treatment with higher cadmium concentrations, while significant correlations between proteases and PMM were detected at lower metal concentrations (Acute10 and Chronic10 and 30 μg Cd/g dry food). In contrast to chronic treatment, egg masses respond more uniformly by reducing PMM during the short-term effect of cadmium. Finally, we can conclude that, as an addition to biochemical and molecular research, PMM can be used for studying the cadmium effects to gain a better insight into the state of the organism under stress conditions.",
publisher = "Lahore: Zoological Society of Pakistan",
journal = "Pakistan Journal of Zoology",
title = "The Influence of Dietary Cadmium on Changes in the Midgut Mass Related to the Mass of Gypsy Moth Larvae",
doi = "10.17582/journal.pjz/20211019131007"
}

Vlahović, M., Matić, D., Mrdaković, M., Ilijin, L., Grčić, A., Filipović, A., Lazarević, J.,& Perić Mataruga, V.. (2022). The Influence of Dietary Cadmium on Changes in the Midgut Mass Related to the Mass of Gypsy Moth Larvae. in Pakistan Journal of Zoology
Lahore: Zoological Society of Pakistan..
https://doi.org/10.17582/journal.pjz/20211019131007

Vlahović M, Matić D, Mrdaković M, Ilijin L, Grčić A, Filipović A, Lazarević J, Perić Mataruga V. The Influence of Dietary Cadmium on Changes in the Midgut Mass Related to the Mass of Gypsy Moth Larvae. in Pakistan Journal of Zoology. 2022;.
doi:10.17582/journal.pjz/20211019131007 .

Vlahović, Milena, Matić, Dragana, Mrdaković, Marija, Ilijin, Larisa, Grčić, Anja, Filipović, Aleksandra, Lazarević, Jelica, Perić Mataruga, Vesna, "The Influence of Dietary Cadmium on Changes in the Midgut Mass Related to the Mass of Gypsy Moth Larvae" in Pakistan Journal of Zoology (2022),
https://doi.org/10.17582/journal.pjz/20211019131007 . .

TY  - CONF
AU  - Perić Mataruga, Vesna
AU  - Todorović, Dajana
AU  - Ilijin, Larisa
AU  - Vlahović, Milena
AU  - Mrdaković, Marija
AU  - Matić, Dragana
AU  - Grčić, Anja
AU  - Filipović, Aleksandra
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5314
AB  - Life on Earth exists under the influence of natural magnetic field (geomagnetic field – 25 to 65 μT). Nowadays, this is not the only magnetic field (MF) whose effects we find and endure in nature. Human activities (electrification, industrial and technological development) are sources of several strong artificial MFs. These MFs have a stressful impact on the life processes in the organisms and should be treated as a kind of environmental pollutant that deserves constantly growing attention. Insects are sensitive to Mfs, showing considerable changes in development, neuroendocrine system, genome, metabolism, antioxidative defence, daily activities, orientation, behavior etc. Static magnetic field (SMF) extensively changes free radical production, increases peroxidation processes of lipid bilayers of cell membranes and expresses a negative influence on insect tissues. Prooxidative stressogenic effect of SMF on insect tissues includes modification of activity of antioxidative enzymes and production of non-enzymatic antioxidants.
The aim of our research was to evaluate differences in fitness components (survival rate, larval mass, development duration) and antioxidative defence strategy (activity of superoxide dismutase - SOD, catalase - CAT, glutathion S-transferase - GST and whole amount of glutathione - GSH) of male and female Gypsy Moth (Lymantria dispar Linnaeus, 1758) larvae, after the long-term exposure to SMF (230mT).
Newly hatched male and female larvae of L. dispar were exposed to a magnetic field of approximately 230 mT generated by a static double U-shaped magnet (Raytheon, model 6002). It consists of two symmetric halves. Magnetic field has relatively homogenous strength and field was measured by a gausmeter (HIRST – GAUSSMETER GM 05, with probe PT 2837 – Hirst Magnetic Instruments LTD, Tesla House, Tregoniggie, Cornwell, UK). Determination of the differences in activities of antioxidative enzymes and the amount of GSH was done in whole larvae homogenates. SOD was conducted according to the methods described by Mistra & Fridovich (1972). CAT activity was determined according to the method by Beutler (1982). GR activity was measured according to Glatzle et al. (1974). GST (antioxidative function) activity was determined according to the method by Habig et al. (1974). Determination of the concentrations of reduced GSH was conducted according to Griffith (1980). We also evaluated fitness components daily.
The study provides information on the effects of the long-term exposure of male and female L. dispar larvae to the SMF. Such exposure induces significant alterations in their strategy of antioxidative defence that are strikingly sex-specific. Increasing the knowledge of effects of SMF exposure in evolutionary simpler organisms may be the basis for understanding its action in higher organisms and humans.
PB  - Niš: RAD Centre
C3  - Book of abstracts:10th Jubilee International Conference on Radiation in Various Fields of Research: RAD 2022; 2022 Jul 25-29; Herceg Novi, Montenegro
T1  - Sex-specific antioxidative strategy and fitness components of Lymantria dispar L. in static magnetic field
SP  - 3
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5314
ER  - 

@conference{
author = "Perić Mataruga, Vesna and Todorović, Dajana and Ilijin, Larisa and Vlahović, Milena and Mrdaković, Marija and Matić, Dragana and Grčić, Anja and Filipović, Aleksandra",
year = "2022",
abstract = "Life on Earth exists under the influence of natural magnetic field (geomagnetic field – 25 to 65 μT). Nowadays, this is not the only magnetic field (MF) whose effects we find and endure in nature. Human activities (electrification, industrial and technological development) are sources of several strong artificial MFs. These MFs have a stressful impact on the life processes in the organisms and should be treated as a kind of environmental pollutant that deserves constantly growing attention. Insects are sensitive to Mfs, showing considerable changes in development, neuroendocrine system, genome, metabolism, antioxidative defence, daily activities, orientation, behavior etc. Static magnetic field (SMF) extensively changes free radical production, increases peroxidation processes of lipid bilayers of cell membranes and expresses a negative influence on insect tissues. Prooxidative stressogenic effect of SMF on insect tissues includes modification of activity of antioxidative enzymes and production of non-enzymatic antioxidants.
The aim of our research was to evaluate differences in fitness components (survival rate, larval mass, development duration) and antioxidative defence strategy (activity of superoxide dismutase - SOD, catalase - CAT, glutathion S-transferase - GST and whole amount of glutathione - GSH) of male and female Gypsy Moth (Lymantria dispar Linnaeus, 1758) larvae, after the long-term exposure to SMF (230mT).
Newly hatched male and female larvae of L. dispar were exposed to a magnetic field of approximately 230 mT generated by a static double U-shaped magnet (Raytheon, model 6002). It consists of two symmetric halves. Magnetic field has relatively homogenous strength and field was measured by a gausmeter (HIRST – GAUSSMETER GM 05, with probe PT 2837 – Hirst Magnetic Instruments LTD, Tesla House, Tregoniggie, Cornwell, UK). Determination of the differences in activities of antioxidative enzymes and the amount of GSH was done in whole larvae homogenates. SOD was conducted according to the methods described by Mistra & Fridovich (1972). CAT activity was determined according to the method by Beutler (1982). GR activity was measured according to Glatzle et al. (1974). GST (antioxidative function) activity was determined according to the method by Habig et al. (1974). Determination of the concentrations of reduced GSH was conducted according to Griffith (1980). We also evaluated fitness components daily.
The study provides information on the effects of the long-term exposure of male and female L. dispar larvae to the SMF. Such exposure induces significant alterations in their strategy of antioxidative defence that are strikingly sex-specific. Increasing the knowledge of effects of SMF exposure in evolutionary simpler organisms may be the basis for understanding its action in higher organisms and humans.",
publisher = "Niš: RAD Centre",
journal = "Book of abstracts:10th Jubilee International Conference on Radiation in Various Fields of Research: RAD 2022; 2022 Jul 25-29; Herceg Novi, Montenegro",
title = "Sex-specific antioxidative strategy and fitness components of Lymantria dispar L. in static magnetic field",
pages = "3",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5314"
}

Perić Mataruga, V., Todorović, D., Ilijin, L., Vlahović, M., Mrdaković, M., Matić, D., Grčić, A.,& Filipović, A.. (2022). Sex-specific antioxidative strategy and fitness components of Lymantria dispar L. in static magnetic field. in Book of abstracts:10th Jubilee International Conference on Radiation in Various Fields of Research: RAD 2022; 2022 Jul 25-29; Herceg Novi, Montenegro
Niš: RAD Centre., 3.
https://hdl.handle.net/21.15107/rcub_ibiss_5314

Perić Mataruga V, Todorović D, Ilijin L, Vlahović M, Mrdaković M, Matić D, Grčić A, Filipović A. Sex-specific antioxidative strategy and fitness components of Lymantria dispar L. in static magnetic field. in Book of abstracts:10th Jubilee International Conference on Radiation in Various Fields of Research: RAD 2022; 2022 Jul 25-29; Herceg Novi, Montenegro. 2022;:3.
https://hdl.handle.net/21.15107/rcub_ibiss_5314 .

Perić Mataruga, Vesna, Todorović, Dajana, Ilijin, Larisa, Vlahović, Milena, Mrdaković, Marija, Matić, Dragana, Grčić, Anja, Filipović, Aleksandra, "Sex-specific antioxidative strategy and fitness components of Lymantria dispar L. in static magnetic field" in Book of abstracts:10th Jubilee International Conference on Radiation in Various Fields of Research: RAD 2022; 2022 Jul 25-29; Herceg Novi, Montenegro (2022):3,
https://hdl.handle.net/21.15107/rcub_ibiss_5314 .

TY  - CONF
AU  - Todorović, Dajana
AU  - Ilijin, Larisa
AU  - Vlahović, Milena
AU  - Mrdaković, Marija
AU  - Petković, Branka
AU  - Matić, Dragana
AU  - Grčić, Anja
AU  - Filipović, Aleksandra
AU  - Perić Mataruga, Vesna
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5313
AB  - Magnetic fields are all around us: in the Universe, on the Earth, among and within organisms, cells, molecules, and single atoms. Fast technological development and modernization of life, increase the level of artificial magnetic fields, thus affecting every living beings. Hence, exposure of organisms to omnipresent magnetic field could represent important stressogenic factor for them. Magnetic sensitivity is very well documented in insects, but there is little research of the effects of magnetic fields on antioxidative defense during their development. The main purpose of this work was to evaluate the effects of static magnetic field (SMF, 230 mT) on antioxidative defense, as well as life history traits of larval instars (from 1st to 4th) of Lymantria dispar L. (Lepidoptera). The activity of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), and glutathione-S transferase (GST) in each larval instar was measured spectrophotometrically. Life history traits (mortality, larval mass and development duration) were monitored daily. Magnetic field increased the activity of SOD, CAT, GR, and GST in all larval instars, but these changes were significant only in older larval instars (3rd and 4th), except CAT. During the larval development, mortality was higher in SMF group than the control. Also, we observed a significant increase in mass of 4th larval instar in magnetic field compared to the control. On the other hand, we did not detect a significant influence of SMF on duration of larval instars. The obtained results demonstrate differences in MF susceptibility of younger and older larval instars of L. dispar. In conclusion, the applied magnetic field could be considered as a potential stressor influencing life history traits, as well as examined antioxidative biomarkers.
PB  - Niš: RAD Centre
C3  - Book of abstracts:10th Jubilee International Conference on Radiation in Various Fields of Research: RAD 2022; 2022 Jul 25-29; Herceg Novi, Montenegro
T1  - Antioxidative defense and life history traits of four larval instars of Lymantria dispar L. in static magnetic field
SP  - 2
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5313
ER  - 

@conference{
author = "Todorović, Dajana and Ilijin, Larisa and Vlahović, Milena and Mrdaković, Marija and Petković, Branka and Matić, Dragana and Grčić, Anja and Filipović, Aleksandra and Perić Mataruga, Vesna",
year = "2022",
abstract = "Magnetic fields are all around us: in the Universe, on the Earth, among and within organisms, cells, molecules, and single atoms. Fast technological development and modernization of life, increase the level of artificial magnetic fields, thus affecting every living beings. Hence, exposure of organisms to omnipresent magnetic field could represent important stressogenic factor for them. Magnetic sensitivity is very well documented in insects, but there is little research of the effects of magnetic fields on antioxidative defense during their development. The main purpose of this work was to evaluate the effects of static magnetic field (SMF, 230 mT) on antioxidative defense, as well as life history traits of larval instars (from 1st to 4th) of Lymantria dispar L. (Lepidoptera). The activity of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), and glutathione-S transferase (GST) in each larval instar was measured spectrophotometrically. Life history traits (mortality, larval mass and development duration) were monitored daily. Magnetic field increased the activity of SOD, CAT, GR, and GST in all larval instars, but these changes were significant only in older larval instars (3rd and 4th), except CAT. During the larval development, mortality was higher in SMF group than the control. Also, we observed a significant increase in mass of 4th larval instar in magnetic field compared to the control. On the other hand, we did not detect a significant influence of SMF on duration of larval instars. The obtained results demonstrate differences in MF susceptibility of younger and older larval instars of L. dispar. In conclusion, the applied magnetic field could be considered as a potential stressor influencing life history traits, as well as examined antioxidative biomarkers.",
publisher = "Niš: RAD Centre",
journal = "Book of abstracts:10th Jubilee International Conference on Radiation in Various Fields of Research: RAD 2022; 2022 Jul 25-29; Herceg Novi, Montenegro",
title = "Antioxidative defense and life history traits of four larval instars of Lymantria dispar L. in static magnetic field",
pages = "2",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5313"
}

Todorović, D., Ilijin, L., Vlahović, M., Mrdaković, M., Petković, B., Matić, D., Grčić, A., Filipović, A.,& Perić Mataruga, V.. (2022). Antioxidative defense and life history traits of four larval instars of Lymantria dispar L. in static magnetic field. in Book of abstracts:10th Jubilee International Conference on Radiation in Various Fields of Research: RAD 2022; 2022 Jul 25-29; Herceg Novi, Montenegro
Niš: RAD Centre., 2.
https://hdl.handle.net/21.15107/rcub_ibiss_5313

Todorović D, Ilijin L, Vlahović M, Mrdaković M, Petković B, Matić D, Grčić A, Filipović A, Perić Mataruga V. Antioxidative defense and life history traits of four larval instars of Lymantria dispar L. in static magnetic field. in Book of abstracts:10th Jubilee International Conference on Radiation in Various Fields of Research: RAD 2022; 2022 Jul 25-29; Herceg Novi, Montenegro. 2022;:2.
https://hdl.handle.net/21.15107/rcub_ibiss_5313 .

Todorović, Dajana, Ilijin, Larisa, Vlahović, Milena, Mrdaković, Marija, Petković, Branka, Matić, Dragana, Grčić, Anja, Filipović, Aleksandra, Perić Mataruga, Vesna, "Antioxidative defense and life history traits of four larval instars of Lymantria dispar L. in static magnetic field" in Book of abstracts:10th Jubilee International Conference on Radiation in Various Fields of Research: RAD 2022; 2022 Jul 25-29; Herceg Novi, Montenegro (2022):2,
https://hdl.handle.net/21.15107/rcub_ibiss_5313 .

TY  - CONF
AU  - Filipović, Aleksandra
AU  - Mrdaković, Marija
AU  - Ilijin, Larisa
AU  - Grčić, Anja
AU  - Matić, Dragana
AU  - Todorović, Dajana
AU  - Perić Mataruga, Vesna
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5133
AB  - Fluoranten pripada grupi policikličnih aromatičnih ugljovodonika (PAH),
perzistentnih organskih polutanata životne sredine. Jedan je od dominantnih
PAH u lišću biljaka1 optimalnih u ishrani polifagnih vrsta insekata Lymantria
dispar L. i Euproctis chrysorrhoea L. Štetni efekti PAH opisani kod različitih
biljaka,2 mogu uticati na fitofagne insekte. Cilj rada je ispitivanje aktivnosti
alkalnih (ALP) i ukupnih kiselih (ACP) fosfataza srednjeg creva larvi ovih
vrsta, hronično izlaganih sredinski relevantnim koncentracijama fluorantena
dodatog hrani. Razlike u ekspresiji izoformi fosfataza, i zavisnost intenziteta
ekspresije pojedinih izoformi od primenjene koncentracije polutanta,
omogućile su efikasne specifične aktivnosti ovih enzima kod larvi gubara
tretiranih grupa. Razlike specifičnih aktivnosti ALP i veći intenzitet
ekspresije izoforme enzima, uočeni su kod larvi žutotrbe izlaganih
fluorantenu. Značajno povećanje specifične aktivnosti ACP i ekspresija 4
izoforme enzima, detektovani su kod larvi žutotrbe izlaganih manjoj
koncentraciji fluorantena. Izoforma enzima detektovana samo u ovoj grupi
larvi mogla bi biti nosilac specifične aktivnosti ACP i uzrok razlika u
odgovoru ovog enzima kod larvi ispitivanih vrsta na prisustvo fluorantena. Na
značaj metaboličkih i drugih uloga ovih enzima kod insekata u normalnim
uslovima, ali i u prisustvu stresora,3 ukazuju i specifične aktivnosti i
ekspresije izoformi fosfataza srednjeg creva larvi gubara i žutotrbe,
detektovane u ovom radu.
AB  - Флуорантен припада групи полицикличних ароматичних угљоводоника (PAH), перзистентних органских полутаната животне средине. Један је од доминантних PAH у лишћу биљака1 оптималних у исхрани полифагних врста инсеката Lymantria dispar L. и Euproctis chrysorrhoea L. Штетни ефекти PAH описани код различитих биљака,2 могу утицати на фитофагне инсекте. Циљ рада је испитивање активности алкалних (ALP) и укупних киселих (ACP) фосфатаза средњег црева ларви ових врста, хронично излаганих средински релевантним концентрацијама флуорантена додатог храни. Разлике у експресији изоформи фосфатаза, и зависност интензитета експресије појединих изоформи од примењене концентрације полутанта, омогућиле су ефикасне специфичне активности ових ензима код ларви губара третираних група. Разлике специфичних активности ALP и већи интензитет експресије изоформе ензима, уочени су код ларви жутотрбе излаганих флуорантену. Значајно повећање специфичне активности ACP и експресија 4 изоформе ензима, детектовани су код ларви жутотрбе излаганих мањој концентрацији флуорантена. Изоформа ензима детектована само у овој групи ларви могла би бити носилац специфичне активности ACP и узрок разлика у одговору овог ензима код ларви испитиваних врста на присуство флуорантена. На значај метаболичких и других улога ових ензима код инсеката у нормалним условима, али и у присуству стресора,3 указују и специфичне активности и експресије изоформи фосфатаза средњег црева ларви губара и жутотрбе, детектоване у овом раду.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Uticaj fluorantena na aktivnost alkalnih i ukupnih kiselih fosfataza srednjeg creva larvi gubara (Lymantria dispar L.) i žutotrbe (Euproctis chrysorrhoea L.), (Lepidoptera: Erebidae)
T1  - Утицај флуорантена на активност алкалних и укупних киселих фосфатаза средњег црева ларви губара (Lymantria dispar L.) и жутотрбе (Euproctis chrysorrhoea L.), (Lepidoptera: Erebidae)
SP  - 356
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5133
ER  - 

@conference{
author = "Filipović, Aleksandra and Mrdaković, Marija and Ilijin, Larisa and Grčić, Anja and Matić, Dragana and Todorović, Dajana and Perić Mataruga, Vesna",
year = "2022",
abstract = "Fluoranten pripada grupi policikličnih aromatičnih ugljovodonika (PAH),
perzistentnih organskih polutanata životne sredine. Jedan je od dominantnih
PAH u lišću biljaka1 optimalnih u ishrani polifagnih vrsta insekata Lymantria
dispar L. i Euproctis chrysorrhoea L. Štetni efekti PAH opisani kod različitih
biljaka,2 mogu uticati na fitofagne insekte. Cilj rada je ispitivanje aktivnosti
alkalnih (ALP) i ukupnih kiselih (ACP) fosfataza srednjeg creva larvi ovih
vrsta, hronično izlaganih sredinski relevantnim koncentracijama fluorantena
dodatog hrani. Razlike u ekspresiji izoformi fosfataza, i zavisnost intenziteta
ekspresije pojedinih izoformi od primenjene koncentracije polutanta,
omogućile su efikasne specifične aktivnosti ovih enzima kod larvi gubara
tretiranih grupa. Razlike specifičnih aktivnosti ALP i veći intenzitet
ekspresije izoforme enzima, uočeni su kod larvi žutotrbe izlaganih
fluorantenu. Značajno povećanje specifične aktivnosti ACP i ekspresija 4
izoforme enzima, detektovani su kod larvi žutotrbe izlaganih manjoj
koncentraciji fluorantena. Izoforma enzima detektovana samo u ovoj grupi
larvi mogla bi biti nosilac specifične aktivnosti ACP i uzrok razlika u
odgovoru ovog enzima kod larvi ispitivanih vrsta na prisustvo fluorantena. Na
značaj metaboličkih i drugih uloga ovih enzima kod insekata u normalnim
uslovima, ali i u prisustvu stresora,3 ukazuju i specifične aktivnosti i
ekspresije izoformi fosfataza srednjeg creva larvi gubara i žutotrbe,
detektovane u ovom radu., Флуорантен припада групи полицикличних ароматичних угљоводоника (PAH), перзистентних органских полутаната животне средине. Један је од доминантних PAH у лишћу биљака1 оптималних у исхрани полифагних врста инсеката Lymantria dispar L. и Euproctis chrysorrhoea L. Штетни ефекти PAH описани код различитих биљака,2 могу утицати на фитофагне инсекте. Циљ рада је испитивање активности алкалних (ALP) и укупних киселих (ACP) фосфатаза средњег црева ларви ових врста, хронично излаганих средински релевантним концентрацијама флуорантена додатог храни. Разлике у експресији изоформи фосфатаза, и зависност интензитета експресије појединих изоформи од примењене концентрације полутанта, омогућиле су ефикасне специфичне активности ових ензима код ларви губара третираних група. Разлике специфичних активности ALP и већи интензитет експресије изоформе ензима, уочени су код ларви жутотрбе излаганих флуорантену. Значајно повећање специфичне активности ACP и експресија 4 изоформе ензима, детектовани су код ларви жутотрбе излаганих мањој концентрацији флуорантена. Изоформа ензима детектована само у овој групи ларви могла би бити носилац специфичне активности ACP и узрок разлика у одговору овог ензима код ларви испитиваних врста на присуство флуорантена. На значај метаболичких и других улога ових ензима код инсеката у нормалним условима, али и у присуству стресора,3 указују и специфичне активности и експресије изоформи фосфатаза средњег црева ларви губара и жутотрбе, детектоване у овом раду.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Uticaj fluorantena na aktivnost alkalnih i ukupnih kiselih fosfataza srednjeg creva larvi gubara (Lymantria dispar L.) i žutotrbe (Euproctis chrysorrhoea L.), (Lepidoptera: Erebidae), Утицај флуорантена на активност алкалних и укупних киселих фосфатаза средњег црева ларви губара (Lymantria dispar L.) и жутотрбе (Euproctis chrysorrhoea L.), (Lepidoptera: Erebidae)",
pages = "356",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5133"
}

Filipović, A., Mrdaković, M., Ilijin, L., Grčić, A., Matić, D., Todorović, D.,& Perić Mataruga, V.. (2022). Uticaj fluorantena na aktivnost alkalnih i ukupnih kiselih fosfataza srednjeg creva larvi gubara (Lymantria dispar L.) i žutotrbe (Euproctis chrysorrhoea L.), (Lepidoptera: Erebidae). in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 356.
https://hdl.handle.net/21.15107/rcub_ibiss_5133

Filipović A, Mrdaković M, Ilijin L, Grčić A, Matić D, Todorović D, Perić Mataruga V. Uticaj fluorantena na aktivnost alkalnih i ukupnih kiselih fosfataza srednjeg creva larvi gubara (Lymantria dispar L.) i žutotrbe (Euproctis chrysorrhoea L.), (Lepidoptera: Erebidae). in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:356.
https://hdl.handle.net/21.15107/rcub_ibiss_5133 .

Filipović, Aleksandra, Mrdaković, Marija, Ilijin, Larisa, Grčić, Anja, Matić, Dragana, Todorović, Dajana, Perić Mataruga, Vesna, "Uticaj fluorantena na aktivnost alkalnih i ukupnih kiselih fosfataza srednjeg creva larvi gubara (Lymantria dispar L.) i žutotrbe (Euproctis chrysorrhoea L.), (Lepidoptera: Erebidae)" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):356,
https://hdl.handle.net/21.15107/rcub_ibiss_5133 .

TY  - CONF
AU  - Grčić, Anja
AU  - Ilijin, Larisa
AU  - Matić, Dragana
AU  - Filipović, Aleksandra
AU  - Mrdaković, Marija
AU  - Todorović, Dajana
AU  - Perić Mataruga, Vesna
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5132
AB  - Температурни обрасци и таласи загревања атмосфере значајно афектују шумске екосистеме и тешко погађају инсекте, чија телесна температура директно зависи од температуре околине. Променљива клима изазов је инсектима, нарочито у метаболичкким процесима,1 те је стога испитан утицај акутног повећања температуре на 28°С, током 24, 72 и 96 h, са и без опоравка на 23°С, на ензимe средњег црева трипсина и липаза, протеинe и липидe хемолимфе и масу ларви губара (Lymantria dispar L.). Осетљивост параметара процењена је и у односу на популационе адаптације губара у одсуству, односно присуству загађивача у шумским стаништима. Двофакторском анализом варијансе показан је значајан заједнички ефекат температурног третмана и популационог порекла на активности трипсина и липаза, посебно током 24 h на 28ºС са повратком на 23ºС. Концентрација укупних протеина и липида у хемолимфи ларви обе популације значајно је повећана након 72 h на 28ºС и 96 h на 28ºС са опоравком на 23ºС. Температурни третман битно је утицао на пораст масе ларви из оба станишта. Анализом главних компоненти откривене су популационе разлике у осетљивости испитаних параметара на краткотрајне варијације у температури указујући на генерално бољу диференцираност одговора код ларви пореклом из загађеног станишта, као и на популациона раздвајања у доприносу варијабли. Резултати сугеришу да утицај климатских промена на елементе енергетског метаболизма ларви губара има потенцијал да буде значајан, посебно са разматрањем популационих адаптација.
AB  - Temperaturni obrasci i talasi zagrevanja atmosfere značajno afektuju šumske ekosisteme i teško pogađaju insekte, čija telesna temperatura direktno zavisi od temperature okoline. Promenljiva klima izazov je insektima, naročito u metaboličkkim procesima,1 te je stoga ispitan uticaj akutnog povećanja temperature na 28°S, tokom 24, 72 i 96 h, sa i bez oporavka na 23°S, na enzime srednjeg creva tripsina i lipaza, proteine i lipide hemolimfe i masu larvi gubara (Lymantria dispar L.). Osetljivost parametara procenjena je i u odnosu na populacione adaptacije gubara u odsustvu, odnosno prisustvu zagađivača u šumskim staništima. Dvofaktorskom analizom varijanse pokazan je značajan zajednički efekat temperaturnog tretmana i populacionog porekla na aktivnosti tripsina i lipaza, posebno tokom 24 h na 28ºS sa povratkom na 23ºS. Koncentracija ukupnih proteina i lipida u hemolimfi larvi obe populacije značajno je povećana nakon 72 h na 28ºS i 96 h na 28ºS sa oporavkom na 23ºS. Temperaturni tretman bitno je uticao na porast mase larvi iz oba staništa. Analizom glavnih komponenti otkrivene su populacione razlike u osetljivosti ispitanih parametara na kratkotrajne varijacije u temperaturi ukazujući na generalno bolju diferenciranost odgovora kod larvi poreklom iz zagađenog staništa, kao i na populaciona razdvajanja u doprinosu varijabli. Rezultati sugerišu da uticaj klimatskih promena na elemente energetskog metabolizma larvi gubara ima potencijal da bude značajan, posebno sa razmatranjem populacionih adaptacija.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Efekti temperaturnih fluktuacija na specificne aktivnosti tripsina i lipaza srednjeg creva i koncentraciju ukupnih proteina i lipida hemolimfe larvi gubara (Lymantria dispar L.) razlicito adaptiranih na sredinsko zagadjenje
T1  - Ефекти температурних флуктуација на специфичне активности трипсина и липаза средњег црева и концентрацију укупних протеина и липида хемолимфе ларви губара (Lymantria dispar L.) различито адаптираних на срединско загађење
SP  - 357
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5132
ER  - 

@conference{
author = "Grčić, Anja and Ilijin, Larisa and Matić, Dragana and Filipović, Aleksandra and Mrdaković, Marija and Todorović, Dajana and Perić Mataruga, Vesna",
year = "2022",
abstract = "Температурни обрасци и таласи загревања атмосфере значајно афектују шумске екосистеме и тешко погађају инсекте, чија телесна температура директно зависи од температуре околине. Променљива клима изазов је инсектима, нарочито у метаболичкким процесима,1 те је стога испитан утицај акутног повећања температуре на 28°С, током 24, 72 и 96 h, са и без опоравка на 23°С, на ензимe средњег црева трипсина и липаза, протеинe и липидe хемолимфе и масу ларви губара (Lymantria dispar L.). Осетљивост параметара процењена је и у односу на популационе адаптације губара у одсуству, односно присуству загађивача у шумским стаништима. Двофакторском анализом варијансе показан је значајан заједнички ефекат температурног третмана и популационог порекла на активности трипсина и липаза, посебно током 24 h на 28ºС са повратком на 23ºС. Концентрација укупних протеина и липида у хемолимфи ларви обе популације значајно је повећана након 72 h на 28ºС и 96 h на 28ºС са опоравком на 23ºС. Температурни третман битно је утицао на пораст масе ларви из оба станишта. Анализом главних компоненти откривене су популационе разлике у осетљивости испитаних параметара на краткотрајне варијације у температури указујући на генерално бољу диференцираност одговора код ларви пореклом из загађеног станишта, као и на популациона раздвајања у доприносу варијабли. Резултати сугеришу да утицај климатских промена на елементе енергетског метаболизма ларви губара има потенцијал да буде значајан, посебно са разматрањем популационих адаптација., Temperaturni obrasci i talasi zagrevanja atmosfere značajno afektuju šumske ekosisteme i teško pogađaju insekte, čija telesna temperatura direktno zavisi od temperature okoline. Promenljiva klima izazov je insektima, naročito u metaboličkkim procesima,1 te je stoga ispitan uticaj akutnog povećanja temperature na 28°S, tokom 24, 72 i 96 h, sa i bez oporavka na 23°S, na enzime srednjeg creva tripsina i lipaza, proteine i lipide hemolimfe i masu larvi gubara (Lymantria dispar L.). Osetljivost parametara procenjena je i u odnosu na populacione adaptacije gubara u odsustvu, odnosno prisustvu zagađivača u šumskim staništima. Dvofaktorskom analizom varijanse pokazan je značajan zajednički efekat temperaturnog tretmana i populacionog porekla na aktivnosti tripsina i lipaza, posebno tokom 24 h na 28ºS sa povratkom na 23ºS. Koncentracija ukupnih proteina i lipida u hemolimfi larvi obe populacije značajno je povećana nakon 72 h na 28ºS i 96 h na 28ºS sa oporavkom na 23ºS. Temperaturni tretman bitno je uticao na porast mase larvi iz oba staništa. Analizom glavnih komponenti otkrivene su populacione razlike u osetljivosti ispitanih parametara na kratkotrajne varijacije u temperaturi ukazujući na generalno bolju diferenciranost odgovora kod larvi poreklom iz zagađenog staništa, kao i na populaciona razdvajanja u doprinosu varijabli. Rezultati sugerišu da uticaj klimatskih promena na elemente energetskog metabolizma larvi gubara ima potencijal da bude značajan, posebno sa razmatranjem populacionih adaptacija.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Efekti temperaturnih fluktuacija na specificne aktivnosti tripsina i lipaza srednjeg creva i koncentraciju ukupnih proteina i lipida hemolimfe larvi gubara (Lymantria dispar L.) razlicito adaptiranih na sredinsko zagadjenje, Ефекти температурних флуктуација на специфичне активности трипсина и липаза средњег црева и концентрацију укупних протеина и липида хемолимфе ларви губара (Lymantria dispar L.) различито адаптираних на срединско загађење",
pages = "357",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5132"
}

Grčić, A., Ilijin, L., Matić, D., Filipović, A., Mrdaković, M., Todorović, D.,& Perić Mataruga, V.. (2022). Efekti temperaturnih fluktuacija na specificne aktivnosti tripsina i lipaza srednjeg creva i koncentraciju ukupnih proteina i lipida hemolimfe larvi gubara (Lymantria dispar L.) razlicito adaptiranih na sredinsko zagadjenje. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 357.
https://hdl.handle.net/21.15107/rcub_ibiss_5132

Grčić A, Ilijin L, Matić D, Filipović A, Mrdaković M, Todorović D, Perić Mataruga V. Efekti temperaturnih fluktuacija na specificne aktivnosti tripsina i lipaza srednjeg creva i koncentraciju ukupnih proteina i lipida hemolimfe larvi gubara (Lymantria dispar L.) razlicito adaptiranih na sredinsko zagadjenje. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:357.
https://hdl.handle.net/21.15107/rcub_ibiss_5132 .

Grčić, Anja, Ilijin, Larisa, Matić, Dragana, Filipović, Aleksandra, Mrdaković, Marija, Todorović, Dajana, Perić Mataruga, Vesna, "Efekti temperaturnih fluktuacija na specificne aktivnosti tripsina i lipaza srednjeg creva i koncentraciju ukupnih proteina i lipida hemolimfe larvi gubara (Lymantria dispar L.) razlicito adaptiranih na sredinsko zagadjenje" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):357,
https://hdl.handle.net/21.15107/rcub_ibiss_5132 .

TY  - JOUR
AU  - Ilijin, Larisa
AU  - Grčić, Anja
AU  - Mrdaković, Marija
AU  - Vlahović, Milena
AU  - Filipović, Aleksandra
AU  - Matić, Dragana
AU  - Perić Mataruga, Vesna
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4083
AB  - In this paper the effects of increased environmental temperature on the relative growth rate (RGR) and developmental time in 5th instar L. dispar larvae originating from unpolluted and polluted forests were analyzed. As indicators of the level of generated reactive oxygen species in thermal stress, we estimated midgut and hemolymph activity of the antioxidative enzymes, superoxide dismutase (SOD) and catalase (CAT), as well as the detoxifying enzymes glutathione S-transferase (GST), carboxylesterase (CaE) and acetylcholinesterase (AChE) from the midgut and brain tissue. We also examined the influence of induced thermotolerance as a species’ ability to overcome the negative effects of this stressor. In larvae originating from the unpolluted forest, the midgut is the primary location of increased SOD and CAT activity and induced thermotolerance did not modified their activity in either tissue. In larvae from the polluted forest, in both tissues SOD activity was more sensitive to an increased temperature and induced thermotolerance than CAT. Carboxylesterase responded diversely to thermal stress depending on the analyzed tissue regardless the origin of larvae, while the activity of GST and AChE in tissue depended on the origin of larvae. Induced thermotolerance modified the activity of detoxifying enzymes in larvae originating from the polluted forest. Combining the selected parameters into an integrated biomarker response (IBR) the GST, CaE and AChE battery emerged as a potential biomarker for thermal stress in L. dispar larvae.
PB  - Elsevier Ltd.
T2  - Journal of Thermal Biology
T1  - Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress
VL  - 96
DO  - 10.1016/j.jtherbio.2021.102836
SP  - 102836
ER  - 

@article{
author = "Ilijin, Larisa and Grčić, Anja and Mrdaković, Marija and Vlahović, Milena and Filipović, Aleksandra and Matić, Dragana and Perić Mataruga, Vesna",
year = "2021",
abstract = "In this paper the effects of increased environmental temperature on the relative growth rate (RGR) and developmental time in 5th instar L. dispar larvae originating from unpolluted and polluted forests were analyzed. As indicators of the level of generated reactive oxygen species in thermal stress, we estimated midgut and hemolymph activity of the antioxidative enzymes, superoxide dismutase (SOD) and catalase (CAT), as well as the detoxifying enzymes glutathione S-transferase (GST), carboxylesterase (CaE) and acetylcholinesterase (AChE) from the midgut and brain tissue. We also examined the influence of induced thermotolerance as a species’ ability to overcome the negative effects of this stressor. In larvae originating from the unpolluted forest, the midgut is the primary location of increased SOD and CAT activity and induced thermotolerance did not modified their activity in either tissue. In larvae from the polluted forest, in both tissues SOD activity was more sensitive to an increased temperature and induced thermotolerance than CAT. Carboxylesterase responded diversely to thermal stress depending on the analyzed tissue regardless the origin of larvae, while the activity of GST and AChE in tissue depended on the origin of larvae. Induced thermotolerance modified the activity of detoxifying enzymes in larvae originating from the polluted forest. Combining the selected parameters into an integrated biomarker response (IBR) the GST, CaE and AChE battery emerged as a potential biomarker for thermal stress in L. dispar larvae.",
publisher = "Elsevier Ltd.",
journal = "Journal of Thermal Biology",
title = "Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress",
volume = "96",
doi = "10.1016/j.jtherbio.2021.102836",
pages = "102836"
}

Ilijin, L., Grčić, A., Mrdaković, M., Vlahović, M., Filipović, A., Matić, D.,& Perić Mataruga, V.. (2021). Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress. in Journal of Thermal Biology
Elsevier Ltd.., 96, 102836.
https://doi.org/10.1016/j.jtherbio.2021.102836

Ilijin L, Grčić A, Mrdaković M, Vlahović M, Filipović A, Matić D, Perić Mataruga V. Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress. in Journal of Thermal Biology. 2021;96:102836.
doi:10.1016/j.jtherbio.2021.102836 .

Ilijin, Larisa, Grčić, Anja, Mrdaković, Marija, Vlahović, Milena, Filipović, Aleksandra, Matić, Dragana, Perić Mataruga, Vesna, "Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress" in Journal of Thermal Biology, 96 (2021):102836,
https://doi.org/10.1016/j.jtherbio.2021.102836 . .

TY  - JOUR
AU  - Ilijin, Larisa
AU  - Grčić, Anja
AU  - Mrdaković, Marija
AU  - Vlahović, Milena
AU  - Filipović, Aleksandra
AU  - Matić, Dragana
AU  - Perić Mataruga, Vesna
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4083
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4089
AB  - In this paper the effects of increased environmental temperature on the relative growth rate (RGR) and developmental time in 5th instar L. dispar larvae originating from unpolluted and polluted forests were analyzed. As indicators of the level of generated reactive oxygen species in thermal stress, we estimated midgut and hemolymph activity of the antioxidative enzymes, superoxide dismutase (SOD) and catalase (CAT), as well as the detoxifying enzymes glutathione S-transferase (GST), carboxylesterase (CaE) and acetylcholinesterase (AChE) from the midgut and brain tissue. We also examined the influence of induced thermotolerance as a species’ ability to overcome the negative effects of this stressor. In larvae originating from the unpolluted forest, the midgut is the primary location of increased SOD and CAT activity and induced thermotolerance did not modified their activity in either tissue. In larvae from the polluted forest, in both tissues SOD activity was more sensitive to an increased temperature and induced thermotolerance than CAT. Carboxylesterase responded diversely to thermal stress depending on the analyzed tissue regardless the origin of larvae, while the activity of GST and AChE in tissue depended on the origin of larvae. Induced thermotolerance modified the activity of detoxifying enzymes in larvae originating from the polluted forest. Combining the selected parameters into an integrated biomarker response (IBR) the GST, CaE and AChE battery emerged as a potential biomarker for thermal stress in L. dispar larvae.
PB  - Elsevier Ltd.
T2  - Journal of Thermal Biology
T1  - Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress
VL  - 96
DO  - 10.1016/j.jtherbio.2021.102836
SP  - 102836
ER  - 

@article{
author = "Ilijin, Larisa and Grčić, Anja and Mrdaković, Marija and Vlahović, Milena and Filipović, Aleksandra and Matić, Dragana and Perić Mataruga, Vesna",
year = "2021",
abstract = "In this paper the effects of increased environmental temperature on the relative growth rate (RGR) and developmental time in 5th instar L. dispar larvae originating from unpolluted and polluted forests were analyzed. As indicators of the level of generated reactive oxygen species in thermal stress, we estimated midgut and hemolymph activity of the antioxidative enzymes, superoxide dismutase (SOD) and catalase (CAT), as well as the detoxifying enzymes glutathione S-transferase (GST), carboxylesterase (CaE) and acetylcholinesterase (AChE) from the midgut and brain tissue. We also examined the influence of induced thermotolerance as a species’ ability to overcome the negative effects of this stressor. In larvae originating from the unpolluted forest, the midgut is the primary location of increased SOD and CAT activity and induced thermotolerance did not modified their activity in either tissue. In larvae from the polluted forest, in both tissues SOD activity was more sensitive to an increased temperature and induced thermotolerance than CAT. Carboxylesterase responded diversely to thermal stress depending on the analyzed tissue regardless the origin of larvae, while the activity of GST and AChE in tissue depended on the origin of larvae. Induced thermotolerance modified the activity of detoxifying enzymes in larvae originating from the polluted forest. Combining the selected parameters into an integrated biomarker response (IBR) the GST, CaE and AChE battery emerged as a potential biomarker for thermal stress in L. dispar larvae.",
publisher = "Elsevier Ltd.",
journal = "Journal of Thermal Biology",
title = "Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress",
volume = "96",
doi = "10.1016/j.jtherbio.2021.102836",
pages = "102836"
}

Ilijin, L., Grčić, A., Mrdaković, M., Vlahović, M., Filipović, A., Matić, D.,& Perić Mataruga, V.. (2021). Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress. in Journal of Thermal Biology
Elsevier Ltd.., 96, 102836.
https://doi.org/10.1016/j.jtherbio.2021.102836

Ilijin L, Grčić A, Mrdaković M, Vlahović M, Filipović A, Matić D, Perić Mataruga V. Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress. in Journal of Thermal Biology. 2021;96:102836.
doi:10.1016/j.jtherbio.2021.102836 .

Ilijin, Larisa, Grčić, Anja, Mrdaković, Marija, Vlahović, Milena, Filipović, Aleksandra, Matić, Dragana, Perić Mataruga, Vesna, "Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress" in Journal of Thermal Biology, 96 (2021):102836,
https://doi.org/10.1016/j.jtherbio.2021.102836 . .

TY  - JOUR
AU  - Ilijin, Larisa
AU  - Mrdaković, Marija
AU  - Todorović, Dajana
AU  - Vlahović, Milena
AU  - Grčić, Anja
AU  - Filipović, Aleksandra
AU  - Perić Mataruga, Vesna
PY  - 2021
UR  - http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000300811&tlng=en
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4409
AB  - In this paper, we analyzed the effects of chronic exposure (5 months) to static magnetic field (110 mT; SMF) and extremely low frequency magnetic field (ELF MF; 10 mT, 50 Hz) on Blaptica dubia nymphs. We have examined acetylcholinesterase (AChE) activity and heat shock protein 70 (HSP70) level, two sensitive biomarkers of stress in terrestrial insects. Relative growth rate (RGR), as a life history trait, was estimated. AChE activity was determined spectrophotometrically and HSP70 levels were quantified using indirect non-competitive ELISA and Western blotting. Calculated RGR was significantly changed upon exposure to both types of ambiental MFs. The effects of chronic exposure of B. dubia nymphs to SMF and ELF MF (50 Hz) were observed as decreased activity of AChE. The increased level of HSP70 was present only after exposure to SMF. The strength of ELF MF was most likely below the energy level needed to induce the expression of this stress protein. Different patterns of the expression of two HSP70 isoforms, where isoform 2 was sensitive only to SMF, are most likely a possibly switch - off in the expression of constitutive and/or inducible HSP70 isoforms.
T2  - Anais da Academia Brasileira de Ciencias
T1  - Biological effects of chronic exposure of Blaptica dubia (Blattodea: Blaberidae) nymphs to static and extremely low frequency magnetic fields.
IS  - 2
VL  - 93
DO  - 10.1590/0001-3765202120190118
SP  - e20190118
ER  - 

@article{
author = "Ilijin, Larisa and Mrdaković, Marija and Todorović, Dajana and Vlahović, Milena and Grčić, Anja and Filipović, Aleksandra and Perić Mataruga, Vesna",
year = "2021",
abstract = "In this paper, we analyzed the effects of chronic exposure (5 months) to static magnetic field (110 mT; SMF) and extremely low frequency magnetic field (ELF MF; 10 mT, 50 Hz) on Blaptica dubia nymphs. We have examined acetylcholinesterase (AChE) activity and heat shock protein 70 (HSP70) level, two sensitive biomarkers of stress in terrestrial insects. Relative growth rate (RGR), as a life history trait, was estimated. AChE activity was determined spectrophotometrically and HSP70 levels were quantified using indirect non-competitive ELISA and Western blotting. Calculated RGR was significantly changed upon exposure to both types of ambiental MFs. The effects of chronic exposure of B. dubia nymphs to SMF and ELF MF (50 Hz) were observed as decreased activity of AChE. The increased level of HSP70 was present only after exposure to SMF. The strength of ELF MF was most likely below the energy level needed to induce the expression of this stress protein. Different patterns of the expression of two HSP70 isoforms, where isoform 2 was sensitive only to SMF, are most likely a possibly switch - off in the expression of constitutive and/or inducible HSP70 isoforms.",
journal = "Anais da Academia Brasileira de Ciencias",
title = "Biological effects of chronic exposure of Blaptica dubia (Blattodea: Blaberidae) nymphs to static and extremely low frequency magnetic fields.",
number = "2",
volume = "93",
doi = "10.1590/0001-3765202120190118",
pages = "e20190118"
}

Ilijin, L., Mrdaković, M., Todorović, D., Vlahović, M., Grčić, A., Filipović, A.,& Perić Mataruga, V.. (2021). Biological effects of chronic exposure of Blaptica dubia (Blattodea: Blaberidae) nymphs to static and extremely low frequency magnetic fields.. in Anais da Academia Brasileira de Ciencias, 93(2), e20190118.
https://doi.org/10.1590/0001-3765202120190118

Ilijin L, Mrdaković M, Todorović D, Vlahović M, Grčić A, Filipović A, Perić Mataruga V. Biological effects of chronic exposure of Blaptica dubia (Blattodea: Blaberidae) nymphs to static and extremely low frequency magnetic fields.. in Anais da Academia Brasileira de Ciencias. 2021;93(2):e20190118.
doi:10.1590/0001-3765202120190118 .

Ilijin, Larisa, Mrdaković, Marija, Todorović, Dajana, Vlahović, Milena, Grčić, Anja, Filipović, Aleksandra, Perić Mataruga, Vesna, "Biological effects of chronic exposure of Blaptica dubia (Blattodea: Blaberidae) nymphs to static and extremely low frequency magnetic fields." in Anais da Academia Brasileira de Ciencias, 93, no. 2 (2021):e20190118,
https://doi.org/10.1590/0001-3765202120190118 . .

TY  - JOUR
AU  - Matić, Dragana
AU  - Vlahović, Milena
AU  - Ilijin, Larisa
AU  - Grčić, Anja
AU  - Filipović, Aleksandra
AU  - Todorović, Dajana
AU  - Perić Mataruga, Vesna
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S153204562100199X
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4480
AB  - Cadmium (Cd) presence in terrestrial ecosystems is a serious threat that requires continuous development of biomonitoring tools. Ideally, a suitable biomarker of exposure should respond to the toxicant consistently in different populations regardless of previous exposure to pollution. Here we considered the activities and isoform patterns of certain proteases and acid phosphatases (ACP) in the midgut of Lymantria dispar larvae as well as the integrated biomarker response (IBR) for application in Cd biomonitoring. We compared the responses of caterpillars originating from unpolluted and polluted localities after they had been chronically subjected to dietary Cd (50 and 100 μg Cd/g dry food). The population inhabiting the unpolluted forest was far more sensitive to Cd exposure as the activities of total proteases, trypsin (TRY) and leucine aminopeptidase (LAP) were mostly reduced while the activities of total and non-lysosomal ACP were increased. Non-lysosomal ACP activity was elevated in larvae from the contaminated site in response to the higher Cd concentration. Exposure to the metal resulted in numerous alterations in the pattern of enzyme isoforms, but the responses of the two populations were similar except that larvae from the polluted locality were more tolerant to the lower Cd concentration. Non-lysosomal ACP activity and the appearance of ACP isoforms 4 and 5 together with the IBR index are the most promising indicators of Cd presence, potentially applicable even in populations with a history of exposure to pollution. TRY and total ACP activities could be used to monitor populations at uncontaminated localities.
PB  - Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Implications of long-term exposure of a Lymantria dispar L. population to pollution for the response of larval midgut proteases and acid phosphatases to chronic cadmium treatment.
VL  - 250
DO  - 10.1016/j.cbpc.2021.109172
SP  - 109172
ER  - 

@article{
author = "Matić, Dragana and Vlahović, Milena and Ilijin, Larisa and Grčić, Anja and Filipović, Aleksandra and Todorović, Dajana and Perić Mataruga, Vesna",
year = "2021",
abstract = "Cadmium (Cd) presence in terrestrial ecosystems is a serious threat that requires continuous development of biomonitoring tools. Ideally, a suitable biomarker of exposure should respond to the toxicant consistently in different populations regardless of previous exposure to pollution. Here we considered the activities and isoform patterns of certain proteases and acid phosphatases (ACP) in the midgut of Lymantria dispar larvae as well as the integrated biomarker response (IBR) for application in Cd biomonitoring. We compared the responses of caterpillars originating from unpolluted and polluted localities after they had been chronically subjected to dietary Cd (50 and 100 μg Cd/g dry food). The population inhabiting the unpolluted forest was far more sensitive to Cd exposure as the activities of total proteases, trypsin (TRY) and leucine aminopeptidase (LAP) were mostly reduced while the activities of total and non-lysosomal ACP were increased. Non-lysosomal ACP activity was elevated in larvae from the contaminated site in response to the higher Cd concentration. Exposure to the metal resulted in numerous alterations in the pattern of enzyme isoforms, but the responses of the two populations were similar except that larvae from the polluted locality were more tolerant to the lower Cd concentration. Non-lysosomal ACP activity and the appearance of ACP isoforms 4 and 5 together with the IBR index are the most promising indicators of Cd presence, potentially applicable even in populations with a history of exposure to pollution. TRY and total ACP activities could be used to monitor populations at uncontaminated localities.",
publisher = "Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Implications of long-term exposure of a Lymantria dispar L. population to pollution for the response of larval midgut proteases and acid phosphatases to chronic cadmium treatment.",
volume = "250",
doi = "10.1016/j.cbpc.2021.109172",
pages = "109172"
}

Matić, D., Vlahović, M., Ilijin, L., Grčić, A., Filipović, A., Todorović, D.,& Perić Mataruga, V.. (2021). Implications of long-term exposure of a Lymantria dispar L. population to pollution for the response of larval midgut proteases and acid phosphatases to chronic cadmium treatment.. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
Elsevier Inc.., 250, 109172.
https://doi.org/10.1016/j.cbpc.2021.109172

Matić D, Vlahović M, Ilijin L, Grčić A, Filipović A, Todorović D, Perić Mataruga V. Implications of long-term exposure of a Lymantria dispar L. population to pollution for the response of larval midgut proteases and acid phosphatases to chronic cadmium treatment.. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2021;250:109172.
doi:10.1016/j.cbpc.2021.109172 .

Matić, Dragana, Vlahović, Milena, Ilijin, Larisa, Grčić, Anja, Filipović, Aleksandra, Todorović, Dajana, Perić Mataruga, Vesna, "Implications of long-term exposure of a Lymantria dispar L. population to pollution for the response of larval midgut proteases and acid phosphatases to chronic cadmium treatment." in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 250 (2021):109172,
https://doi.org/10.1016/j.cbpc.2021.109172 . .

TY  - JOUR
AU  - Filipović, Aleksandra
AU  - Mrdaković, Marija
AU  - Ilijin, Larisa
AU  - Grčić, Anja
AU  - Matić, Dragana
AU  - Todorović, Dajana
AU  - Vlahović, Milena
AU  - Perić Mataruga, Vesna
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S1532045621001502
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4482
AB  - Fluoranthene is one of the most abundant polycyclic aromatic hydrocarbon pollutants in the environment and it may accumulate in plant leaves which are the main food source for phytophagous insect species. The aim of this study was to establish the effects of dietary fluoranthene on specific activities of digestive enzymes and expression of their isoforms in the midgut, and the relative growth rates of Lymantria dispar and Euproctis chrysorrhoea larvae. Exposure to fluoranthene led to significantly decreased trypsin activity in the midgut of larvae of both species. Leucine aminopeptidase activity decreased significantly in the midgut of L. dispar larvae exposed to the lower concentration of fluoranthene, but that enzyme activity showed the opposite trend in E. chrysorrhoea larvae. There was no pollutant induced changes in lipase activity in L. dispar, while elevated enzyme activity was recorded in the midgut of E. chrysorrhoea larvae exposed to the lower concentration of fluoranthene. Different patterns of expression of enzyme isoforms were noticed. Relative growth rates of both species significantly decreased in fluoranthene treated larvae. These responses indicate to the significance of relationships between physiological changes and fitness-related traits in L. dispar and E. chrysorrhoea larvae affected by pollutant, and contribute to understanding the mechanisms of their adjustment to stressful conditions.
PB  - New York: Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Effects of fluoranthene on digestive enzymes activity and relative growth rate of larvae of lepidopteran species, Lymantria dispar L. and Euproctis chrysorrhoea L.
VL  - 249
DO  - 10.1016/j.cbpc.2021.109123
SP  - 109123
ER  - 

@article{
author = "Filipović, Aleksandra and Mrdaković, Marija and Ilijin, Larisa and Grčić, Anja and Matić, Dragana and Todorović, Dajana and Vlahović, Milena and Perić Mataruga, Vesna",
year = "2021",
abstract = "Fluoranthene is one of the most abundant polycyclic aromatic hydrocarbon pollutants in the environment and it may accumulate in plant leaves which are the main food source for phytophagous insect species. The aim of this study was to establish the effects of dietary fluoranthene on specific activities of digestive enzymes and expression of their isoforms in the midgut, and the relative growth rates of Lymantria dispar and Euproctis chrysorrhoea larvae. Exposure to fluoranthene led to significantly decreased trypsin activity in the midgut of larvae of both species. Leucine aminopeptidase activity decreased significantly in the midgut of L. dispar larvae exposed to the lower concentration of fluoranthene, but that enzyme activity showed the opposite trend in E. chrysorrhoea larvae. There was no pollutant induced changes in lipase activity in L. dispar, while elevated enzyme activity was recorded in the midgut of E. chrysorrhoea larvae exposed to the lower concentration of fluoranthene. Different patterns of expression of enzyme isoforms were noticed. Relative growth rates of both species significantly decreased in fluoranthene treated larvae. These responses indicate to the significance of relationships between physiological changes and fitness-related traits in L. dispar and E. chrysorrhoea larvae affected by pollutant, and contribute to understanding the mechanisms of their adjustment to stressful conditions.",
publisher = "New York: Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Effects of fluoranthene on digestive enzymes activity and relative growth rate of larvae of lepidopteran species, Lymantria dispar L. and Euproctis chrysorrhoea L.",
volume = "249",
doi = "10.1016/j.cbpc.2021.109123",
pages = "109123"
}

Filipović, A., Mrdaković, M., Ilijin, L., Grčić, A., Matić, D., Todorović, D., Vlahović, M.,& Perić Mataruga, V.. (2021). Effects of fluoranthene on digestive enzymes activity and relative growth rate of larvae of lepidopteran species, Lymantria dispar L. and Euproctis chrysorrhoea L.. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
New York: Elsevier Inc.., 249, 109123.
https://doi.org/10.1016/j.cbpc.2021.109123

Filipović A, Mrdaković M, Ilijin L, Grčić A, Matić D, Todorović D, Vlahović M, Perić Mataruga V. Effects of fluoranthene on digestive enzymes activity and relative growth rate of larvae of lepidopteran species, Lymantria dispar L. and Euproctis chrysorrhoea L.. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2021;249:109123.
doi:10.1016/j.cbpc.2021.109123 .

Filipović, Aleksandra, Mrdaković, Marija, Ilijin, Larisa, Grčić, Anja, Matić, Dragana, Todorović, Dajana, Vlahović, Milena, Perić Mataruga, Vesna, "Effects of fluoranthene on digestive enzymes activity and relative growth rate of larvae of lepidopteran species, Lymantria dispar L. and Euproctis chrysorrhoea L." in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 249 (2021):109123,
https://doi.org/10.1016/j.cbpc.2021.109123 . .

TY  - CONF
AU  - Grčić, Anja
AU  - Ilijin, Larisa
AU  - Filipović, Aleksandra
AU  - Matić, Dragana
AU  - Mrdaković, Marija
AU  - Todorović, Dajana
AU  - Perić Mataruga, Vesna
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4913
AB  - INTRODUCTION: Intense anthropogenic influence led to a significant increase in pollution of the biosphere, in which polycyclic aromatic hydrocarbons, especially benzo[a]pyrene (B[a]P) made a major contribution. Wet and dry deposition gets atmospheric B[a]P on the vegetation, an important sink, and a crucial link for B[a]P bioaccumulation in animals1. The gypsy moth is a phytophagous polyphagous insect that inhabits wide forest areas. Due to its vast appetite, it can pile great amounts of pollutants making it a suitable model system for biomonitoring the adverse effects of B[a]P. The larval midgut is the central metabolic place where trypsin and lipases provide efficient digestion of protein and lipids-rich food, showing sensitivity to chemical pollutants2,3. Molecular parameters can be affected by physiological and environmental factors, so different adaptations of insects to the contaminants should be considered during the assessment of biomarker potential. OBJECTIVES: The aim was to investigate chronic effects of dietary treatment with B[a]P on midgut enzyme activities of trypsin and lipase, as well as the content of total proteins and lipids in hemolymph in gypsy moth larvae from two populations - one from an unpolluted oak forest and the other from a polluted oak forest. Furthermore, we used the method of Integrated Biomarker Response (IBR) to summarize responses of multiple molecular parameters across different tissues to estimate their sensitivity to B[a]P exposure in terms of population origin. METHOD / DESIGN: Gypsy moth egg masses were gathered in two mixed oak forests – Đerdap National Park forest, free of industrial pollution (unpolluted population of larvae, UP), and Bor forest contaminated by various byproducts of the mining industry (polluted population of larvae, PP). From hatching until the sacrifice (third day of the 5th instar) larvae were fed with a diet containing 0 ng (UP 0 ng and PP 0 ng), 5 ng (UP 5 ng and PP 5 ng), or 50 ng (UP 50 ng and PP 50 ng) of B[a]P in 1 g of dry diet. Spectrophotometric assays were used for the determination of specific enzyme activities of trypsin and lipase in the homogenates of the midgut, as well as for the evaluation of total proteins and lipids in the hemolymph of larvae4-8. Two-way ANOVA followed by Tukey’s post-hoc test was used for statistical analyses, conducted in GraphPad Prism 8 (GraphPad Software, Inc., USA). Statistical significance was determined at probability (p)<0.05. Excel software (Microsoft, USA) was used to calculate IBR values and to generate star plots9. RESULTS: The specific activity of trypsin has significantly inhibited after the treatment with lower B[a]P concentration in UP (F=9.412, p=0.0004), while a higher concentration of B[a]P significantly induced lipase activity in the same population of larvae (F=8.382, p=0.0007). These enzymes showed no statistically significant changes in the PP. Hemolymph protein content was significantly affected by the chronic dietary exposure to the higher concentration of B[a]P in both populations of larvae, showing a decrease in the UP, and the elevation in the PP (F=10.16, p=0.0002). Lipid concentration was not significantly changed under the B[a]P influence regarding the control groups (UP/PP 0 ng) but there was a meaningful difference between B[a]P treated larvae among two populations (F=7.16, p=0.019). IBR index increased in a concentration-dependent way only in UP after the chronic exposure to B[a]P and the values were higher than the corresponding ones in the PP (IBR index values - UP 0 ng=0; UP 5 ng=1.62; UP 50 ng=4.84; PP 0 ng=2.01; PP 5 ng=1.10; PP 50 ng=3.08). CONCLUSIONS: Gypsy moth population from the unpolluted forest showed higher sensitivity to the chronic dietary exposure to B[a]P comparing to the population from the polluted forest, especially in terms of trypsin and lipase activity. Hemolymph protein level expressed well correspondence to B[a]P concentration in both populations but with inverse trends. The selected set of Gypsy moth larvae molecular parameters possess a good potential for B[a]P biomonitoring in the populations from unpolluted forest ecosystems.
PB  - Novi Sad: Faculty of Sciences, University of Novi Sad
C3  - Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
T1  - Midgut trypsin and lipase activities, hemolymph protein and lipids levels with integrated biomarker response (Ibr) in Gypsy moth (Lymantria Dispar) larvae from clean and polluted forest after chronic exposure to benzo[a]pyrene
SP  - 65
EP  - 66
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4913
ER  - 

@conference{
editor = "Mimica-Dukić, Neda, Pajević, Slobodanka, Mandić, Anamarija",
author = "Grčić, Anja and Ilijin, Larisa and Filipović, Aleksandra and Matić, Dragana and Mrdaković, Marija and Todorović, Dajana and Perić Mataruga, Vesna",
year = "2021",
abstract = "INTRODUCTION: Intense anthropogenic influence led to a significant increase in pollution of the biosphere, in which polycyclic aromatic hydrocarbons, especially benzo[a]pyrene (B[a]P) made a major contribution. Wet and dry deposition gets atmospheric B[a]P on the vegetation, an important sink, and a crucial link for B[a]P bioaccumulation in animals1. The gypsy moth is a phytophagous polyphagous insect that inhabits wide forest areas. Due to its vast appetite, it can pile great amounts of pollutants making it a suitable model system for biomonitoring the adverse effects of B[a]P. The larval midgut is the central metabolic place where trypsin and lipases provide efficient digestion of protein and lipids-rich food, showing sensitivity to chemical pollutants2,3. Molecular parameters can be affected by physiological and environmental factors, so different adaptations of insects to the contaminants should be considered during the assessment of biomarker potential. OBJECTIVES: The aim was to investigate chronic effects of dietary treatment with B[a]P on midgut enzyme activities of trypsin and lipase, as well as the content of total proteins and lipids in hemolymph in gypsy moth larvae from two populations - one from an unpolluted oak forest and the other from a polluted oak forest. Furthermore, we used the method of Integrated Biomarker Response (IBR) to summarize responses of multiple molecular parameters across different tissues to estimate their sensitivity to B[a]P exposure in terms of population origin. METHOD / DESIGN: Gypsy moth egg masses were gathered in two mixed oak forests – Đerdap National Park forest, free of industrial pollution (unpolluted population of larvae, UP), and Bor forest contaminated by various byproducts of the mining industry (polluted population of larvae, PP). From hatching until the sacrifice (third day of the 5th instar) larvae were fed with a diet containing 0 ng (UP 0 ng and PP 0 ng), 5 ng (UP 5 ng and PP 5 ng), or 50 ng (UP 50 ng and PP 50 ng) of B[a]P in 1 g of dry diet. Spectrophotometric assays were used for the determination of specific enzyme activities of trypsin and lipase in the homogenates of the midgut, as well as for the evaluation of total proteins and lipids in the hemolymph of larvae4-8. Two-way ANOVA followed by Tukey’s post-hoc test was used for statistical analyses, conducted in GraphPad Prism 8 (GraphPad Software, Inc., USA). Statistical significance was determined at probability (p)<0.05. Excel software (Microsoft, USA) was used to calculate IBR values and to generate star plots9. RESULTS: The specific activity of trypsin has significantly inhibited after the treatment with lower B[a]P concentration in UP (F=9.412, p=0.0004), while a higher concentration of B[a]P significantly induced lipase activity in the same population of larvae (F=8.382, p=0.0007). These enzymes showed no statistically significant changes in the PP. Hemolymph protein content was significantly affected by the chronic dietary exposure to the higher concentration of B[a]P in both populations of larvae, showing a decrease in the UP, and the elevation in the PP (F=10.16, p=0.0002). Lipid concentration was not significantly changed under the B[a]P influence regarding the control groups (UP/PP 0 ng) but there was a meaningful difference between B[a]P treated larvae among two populations (F=7.16, p=0.019). IBR index increased in a concentration-dependent way only in UP after the chronic exposure to B[a]P and the values were higher than the corresponding ones in the PP (IBR index values - UP 0 ng=0; UP 5 ng=1.62; UP 50 ng=4.84; PP 0 ng=2.01; PP 5 ng=1.10; PP 50 ng=3.08). CONCLUSIONS: Gypsy moth population from the unpolluted forest showed higher sensitivity to the chronic dietary exposure to B[a]P comparing to the population from the polluted forest, especially in terms of trypsin and lipase activity. Hemolymph protein level expressed well correspondence to B[a]P concentration in both populations but with inverse trends. The selected set of Gypsy moth larvae molecular parameters possess a good potential for B[a]P biomonitoring in the populations from unpolluted forest ecosystems.",
publisher = "Novi Sad: Faculty of Sciences, University of Novi Sad",
journal = "Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia",
title = "Midgut trypsin and lipase activities, hemolymph protein and lipids levels with integrated biomarker response (Ibr) in Gypsy moth (Lymantria Dispar) larvae from clean and polluted forest after chronic exposure to benzo[a]pyrene",
pages = "65-66",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4913"
}

Mimica-Dukić, N., Pajević, S., Mandić, A., Grčić, A., Ilijin, L., Filipović, A., Matić, D., Mrdaković, M., Todorović, D.,& Perić Mataruga, V.. (2021). Midgut trypsin and lipase activities, hemolymph protein and lipids levels with integrated biomarker response (Ibr) in Gypsy moth (Lymantria Dispar) larvae from clean and polluted forest after chronic exposure to benzo[a]pyrene. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
Novi Sad: Faculty of Sciences, University of Novi Sad., 65-66.
https://hdl.handle.net/21.15107/rcub_ibiss_4913

Mimica-Dukić N, Pajević S, Mandić A, Grčić A, Ilijin L, Filipović A, Matić D, Mrdaković M, Todorović D, Perić Mataruga V. Midgut trypsin and lipase activities, hemolymph protein and lipids levels with integrated biomarker response (Ibr) in Gypsy moth (Lymantria Dispar) larvae from clean and polluted forest after chronic exposure to benzo[a]pyrene. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia. 2021;:65-66.
https://hdl.handle.net/21.15107/rcub_ibiss_4913 .

Mimica-Dukić, Neda, Pajević, Slobodanka, Mandić, Anamarija, Grčić, Anja, Ilijin, Larisa, Filipović, Aleksandra, Matić, Dragana, Mrdaković, Marija, Todorović, Dajana, Perić Mataruga, Vesna, "Midgut trypsin and lipase activities, hemolymph protein and lipids levels with integrated biomarker response (Ibr) in Gypsy moth (Lymantria Dispar) larvae from clean and polluted forest after chronic exposure to benzo[a]pyrene" in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia (2021):65-66,
https://hdl.handle.net/21.15107/rcub_ibiss_4913 .

TY  - CONF
AU  - Matić, Dragana
AU  - Vlahović, Milena
AU  - Ilijin, Larisa
AU  - Grčić, Anja
AU  - Filipović, Aleksandra
AU  - Mrdaković, Marija
AU  - Perić Mataruga, Vesna
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4912
AB  - INTRODUCTION:
Cadmium (Cd) levels in the environment have increased during decades of intensive industrial development and urbanization. Lymantria dispar has proved to be a suitable organism indicator to monitor Cd pollution in the forest ecosystems. Since insects accumulate heavy metals predominantly in the gut, it is not surprising that several enzymes in the midgut of L. dispar larvae, including antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT), have been marked as promising biomarkers of Cd presence. Namely, Cd indirectly induces oxidative stress in the cell. However, long-term exposure of the population to pollution often results in increased tolerance and changed sensitivity of biomarkers.
OBJECTIVES:
We aimed to determine specific enzyme activities and isoform patterns of SOD and CAT in the midgut of Lymantria dispar larvae after chronic treatment with Cd. To assess these parameters as biomarkers of Cd exposure, we compared the responses of two populations with different histories of an exposure to pollution.
METHOD / DESIGN:
Egg masses of L. dispar were collected from two localities in Serbia - the uncontaminated forest in Kosmaj Mountain, which is a protected natural resource, and a polluted site near the busy Ibar highway. Larvae were fed wheat germ diet containing 0, 50 or 100 μg Cd/g dry food starting from hatching until they were killed on the 3rd day of the 4th instar. Specific activities of SOD and CAT in the midgut homogenates were determined by spectrophotometric assays. Enzyme isoforms were separated by native polyacrylamide gel electrophoresis. Statistical analyses were performed in GraphPad Prism 7 (GraphPad Software, Inc., USA), where enzyme activities were analyzed by one-way ANOVA followed by Tukey’s post-hoc test. The level of statistical significance was p<0.05.
RESULTS:
Specific activity of SOD was higher in control larvae from the polluted locality compared to the control group from the uncontaminated forest. An exposure to both Cd concentrations decreased SOD activity in larvae from the polluted site. Three SOD isoforms were detected in control groups from both populations. While isoform SOD-2 was absent in the population from Kosmaj after the treatment with higher Cd concentration, both SOD-2 and SOD-3 disappeared in all Cd-treated larvae from the site near the highway. In the population from the unpolluted locality specific activity of CAT was reduced at 100 μg Cd/g dry food, whereas in another population a decrease in enzyme activity was noticed at both Cd concentrations. The same pattern of Cd influence was observed for CAT isoform activity. Only one CAT isoform was present in both control and experimental larvae from both populations.
CONCLUSIONS:
Higher SOD activity in control larvae originating from the site near the highway compared to those from the uncontaminated forest probably indicated the presence of traffic-related pollution that caused oxidative stress. However, neither SOD nor CAT showed activation in response to Cd treatment. A decrease in SOD and CAT activity in both Cd-treated groups in the population from the polluted site could have been a result of the trade-off in favour of the other more efficient defense mechanism(s). Such trade-off might have led to the diminished expression of isoforms SOD-2 and SOD-3. Thus, a decrease in SOD and CAT activities after Cd exposure could be seen as an adaptive strategy of L. dispar population living in chronically polluted habitat. These parameters, with SOD isoform pattern, could be used as biomarkers of Cd exposure in contaminated environments.
PB  - Novi Sad: Faculty of Sciences, University of Novi Sad
C3  - Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
T1  - Effects of chronic dietary cadmium on midgut superoxide dismutase (SOD) and catalase (CAT) in larvae from two Lymantria dispar populations
SP  - 77
EP  - 78
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4912
ER  - 

@conference{
editor = "Mimica-Dukić, Neda, Pajević, Slobodanka, Mandić, Anamarija",
author = "Matić, Dragana and Vlahović, Milena and Ilijin, Larisa and Grčić, Anja and Filipović, Aleksandra and Mrdaković, Marija and Perić Mataruga, Vesna",
year = "2021",
abstract = "INTRODUCTION:
Cadmium (Cd) levels in the environment have increased during decades of intensive industrial development and urbanization. Lymantria dispar has proved to be a suitable organism indicator to monitor Cd pollution in the forest ecosystems. Since insects accumulate heavy metals predominantly in the gut, it is not surprising that several enzymes in the midgut of L. dispar larvae, including antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT), have been marked as promising biomarkers of Cd presence. Namely, Cd indirectly induces oxidative stress in the cell. However, long-term exposure of the population to pollution often results in increased tolerance and changed sensitivity of biomarkers.
OBJECTIVES:
We aimed to determine specific enzyme activities and isoform patterns of SOD and CAT in the midgut of Lymantria dispar larvae after chronic treatment with Cd. To assess these parameters as biomarkers of Cd exposure, we compared the responses of two populations with different histories of an exposure to pollution.
METHOD / DESIGN:
Egg masses of L. dispar were collected from two localities in Serbia - the uncontaminated forest in Kosmaj Mountain, which is a protected natural resource, and a polluted site near the busy Ibar highway. Larvae were fed wheat germ diet containing 0, 50 or 100 μg Cd/g dry food starting from hatching until they were killed on the 3rd day of the 4th instar. Specific activities of SOD and CAT in the midgut homogenates were determined by spectrophotometric assays. Enzyme isoforms were separated by native polyacrylamide gel electrophoresis. Statistical analyses were performed in GraphPad Prism 7 (GraphPad Software, Inc., USA), where enzyme activities were analyzed by one-way ANOVA followed by Tukey’s post-hoc test. The level of statistical significance was p<0.05.
RESULTS:
Specific activity of SOD was higher in control larvae from the polluted locality compared to the control group from the uncontaminated forest. An exposure to both Cd concentrations decreased SOD activity in larvae from the polluted site. Three SOD isoforms were detected in control groups from both populations. While isoform SOD-2 was absent in the population from Kosmaj after the treatment with higher Cd concentration, both SOD-2 and SOD-3 disappeared in all Cd-treated larvae from the site near the highway. In the population from the unpolluted locality specific activity of CAT was reduced at 100 μg Cd/g dry food, whereas in another population a decrease in enzyme activity was noticed at both Cd concentrations. The same pattern of Cd influence was observed for CAT isoform activity. Only one CAT isoform was present in both control and experimental larvae from both populations.
CONCLUSIONS:
Higher SOD activity in control larvae originating from the site near the highway compared to those from the uncontaminated forest probably indicated the presence of traffic-related pollution that caused oxidative stress. However, neither SOD nor CAT showed activation in response to Cd treatment. A decrease in SOD and CAT activity in both Cd-treated groups in the population from the polluted site could have been a result of the trade-off in favour of the other more efficient defense mechanism(s). Such trade-off might have led to the diminished expression of isoforms SOD-2 and SOD-3. Thus, a decrease in SOD and CAT activities after Cd exposure could be seen as an adaptive strategy of L. dispar population living in chronically polluted habitat. These parameters, with SOD isoform pattern, could be used as biomarkers of Cd exposure in contaminated environments.",
publisher = "Novi Sad: Faculty of Sciences, University of Novi Sad",
journal = "Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia",
title = "Effects of chronic dietary cadmium on midgut superoxide dismutase (SOD) and catalase (CAT) in larvae from two Lymantria dispar populations",
pages = "77-78",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4912"
}

Mimica-Dukić, N., Pajević, S., Mandić, A., Matić, D., Vlahović, M., Ilijin, L., Grčić, A., Filipović, A., Mrdaković, M.,& Perić Mataruga, V.. (2021). Effects of chronic dietary cadmium on midgut superoxide dismutase (SOD) and catalase (CAT) in larvae from two Lymantria dispar populations. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
Novi Sad: Faculty of Sciences, University of Novi Sad., 77-78.
https://hdl.handle.net/21.15107/rcub_ibiss_4912

Mimica-Dukić N, Pajević S, Mandić A, Matić D, Vlahović M, Ilijin L, Grčić A, Filipović A, Mrdaković M, Perić Mataruga V. Effects of chronic dietary cadmium on midgut superoxide dismutase (SOD) and catalase (CAT) in larvae from two Lymantria dispar populations. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia. 2021;:77-78.
https://hdl.handle.net/21.15107/rcub_ibiss_4912 .

Mimica-Dukić, Neda, Pajević, Slobodanka, Mandić, Anamarija, Matić, Dragana, Vlahović, Milena, Ilijin, Larisa, Grčić, Anja, Filipović, Aleksandra, Mrdaković, Marija, Perić Mataruga, Vesna, "Effects of chronic dietary cadmium on midgut superoxide dismutase (SOD) and catalase (CAT) in larvae from two Lymantria dispar populations" in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia (2021):77-78,
https://hdl.handle.net/21.15107/rcub_ibiss_4912 .

TY  - CONF
AU  - Ilijin, Larisa
AU  - Grčić, Anja
AU  - Mrdaković, Marija
AU  - Vlahović, Milena
AU  - Matić, Dragana
AU  - Todorović, Dajana
AU  - Perić Mataruga, Vesna
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4784
AB  - INTRODUCTION: Environmental temperature has a direct impact on the development of phytophagous insects, and an indirect, through influence on their host plant composition. Alkaline phosphatases (ALP) and total acid phosphatases (tot ACP) are midgut enzymes included in metabolic processes. Previous contact of population with various stressors, but also their ability to overcome the effects of the raised temperature (thermotolerance), can modify the response of these enzymes to increased environmental temperature.
OBJECTIVES: Our aim was to compare the differences in responses of midgut ALP and tot ACP and expression of their isoforms to increased environmental temperature with and without induced thermotolerance, in gypsy moth 5th instar caterpillars from unpolluted and polluted habitats.
METHOD / DESIGN: Caterpillars were hatched from egg masses collected in unpolluted (UP population) and polluted forest (PP population). They were reared at 23°C (PP23 and UP 23) and 28°C (PP28 and UP28) until 3rd day of 5th instar. In both populations a group of individuals were exposed to 28°C for 24 h (induced thermotolerance) at the beginning of the 4th instar. Afterwards they were returned to 23°C until the sacrification (PP23In and UP23In) or exposed to 28°C for 72h before sacrification on the 3rd day of 5th instar (PP28In and UP28In). The activity of enzymes was measured spectrophotometrically, using p-nitrophenyl phosphate (pNPP) as substrate, under alkaline conditions for ALP and acid conditions for tot ACP. Isoforms of both enzymes were detected on 12% polyacrylamide gel native PAGE. 
RESULTS: In UP groups, midgut ALP showed increased activity upon exposure to 28°C, with and without induced thermotolerance, while in PP caterpillars induced thermotolerance was the only factor that elevated ALP activity. Two way ANOVA analysis revealed that the interaction of temperature treatments and population origin (unpolluted vs polluted forest) was extremely significant (F3,67=27.6, p<0.0001) for changes in midgut ALP activity, as well as the individual influence of increased temperature (F3,67=30.9, p<0.0001) and the origin of the population (F1,67=28.6, p<0.0001). Three ALP isoforms were detected. Isoform 1 was present only in PP groups exposed to 28°C, second is present in all experimental groups, and the third showed lower band density in PP treatments in comparison to UP. In UP23In tot ACP activity was elevated, while in PP treatments it was decreased. The interaction of temperature and population origin was extremely significant for tot ACP activity (two way ANOVA, F3,72=10.48, p<0.0001), while their individual influence was not. Four isoforms of tot ACP were detected on gel. Isoform 1 was present only in PP groups, isoform 2 has higher density in both populations and all treatments in comparison to controls. High band density of isoform 3 is present in all experimental groups, while induced thermotolerance and increased temperature, in both population, increased band density of isoform 4.
CONCLUSIONS: Increased environmental temperature and induced thermotolerance have different effects on the activity of both enzymes in caterpillars from unpolluted and polluted habitats. ALP activity was more sensitive to thermal treatments in individuals originating from unpolluted forests, in comparison to those from polluted habitats, where, on the other hand, completely new isoform was detected upon exposure to increased temperature. Tot ACP activity was decreased in all treatments in caterpillars from polluted habitats and a new isoform band was detected on native gels, while in those from unpolluted forest, induced thermotolerance had the effect on the activity of tot ACP. Obtained results indicate the differences in sensitivity to increased environmental temperature between populations with different histories of exposure to pollution and that they must be considered as well.
PB  - Novi Sad: Faculty of Sciences, University of Novi Sad
C3  - The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021
T1  - Sensitivity of midgut phosphatases to thermal stress in Gypsy moth (Lymantria Dispar) caterpillars
SP  - 64
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4784
ER  - 

@conference{
author = "Ilijin, Larisa and Grčić, Anja and Mrdaković, Marija and Vlahović, Milena and Matić, Dragana and Todorović, Dajana and Perić Mataruga, Vesna",
year = "2021",
abstract = "INTRODUCTION: Environmental temperature has a direct impact on the development of phytophagous insects, and an indirect, through influence on their host plant composition. Alkaline phosphatases (ALP) and total acid phosphatases (tot ACP) are midgut enzymes included in metabolic processes. Previous contact of population with various stressors, but also their ability to overcome the effects of the raised temperature (thermotolerance), can modify the response of these enzymes to increased environmental temperature.
OBJECTIVES: Our aim was to compare the differences in responses of midgut ALP and tot ACP and expression of their isoforms to increased environmental temperature with and without induced thermotolerance, in gypsy moth 5th instar caterpillars from unpolluted and polluted habitats.
METHOD / DESIGN: Caterpillars were hatched from egg masses collected in unpolluted (UP population) and polluted forest (PP population). They were reared at 23°C (PP23 and UP 23) and 28°C (PP28 and UP28) until 3rd day of 5th instar. In both populations a group of individuals were exposed to 28°C for 24 h (induced thermotolerance) at the beginning of the 4th instar. Afterwards they were returned to 23°C until the sacrification (PP23In and UP23In) or exposed to 28°C for 72h before sacrification on the 3rd day of 5th instar (PP28In and UP28In). The activity of enzymes was measured spectrophotometrically, using p-nitrophenyl phosphate (pNPP) as substrate, under alkaline conditions for ALP and acid conditions for tot ACP. Isoforms of both enzymes were detected on 12% polyacrylamide gel native PAGE. 
RESULTS: In UP groups, midgut ALP showed increased activity upon exposure to 28°C, with and without induced thermotolerance, while in PP caterpillars induced thermotolerance was the only factor that elevated ALP activity. Two way ANOVA analysis revealed that the interaction of temperature treatments and population origin (unpolluted vs polluted forest) was extremely significant (F3,67=27.6, p<0.0001) for changes in midgut ALP activity, as well as the individual influence of increased temperature (F3,67=30.9, p<0.0001) and the origin of the population (F1,67=28.6, p<0.0001). Three ALP isoforms were detected. Isoform 1 was present only in PP groups exposed to 28°C, second is present in all experimental groups, and the third showed lower band density in PP treatments in comparison to UP. In UP23In tot ACP activity was elevated, while in PP treatments it was decreased. The interaction of temperature and population origin was extremely significant for tot ACP activity (two way ANOVA, F3,72=10.48, p<0.0001), while their individual influence was not. Four isoforms of tot ACP were detected on gel. Isoform 1 was present only in PP groups, isoform 2 has higher density in both populations and all treatments in comparison to controls. High band density of isoform 3 is present in all experimental groups, while induced thermotolerance and increased temperature, in both population, increased band density of isoform 4.
CONCLUSIONS: Increased environmental temperature and induced thermotolerance have different effects on the activity of both enzymes in caterpillars from unpolluted and polluted habitats. ALP activity was more sensitive to thermal treatments in individuals originating from unpolluted forests, in comparison to those from polluted habitats, where, on the other hand, completely new isoform was detected upon exposure to increased temperature. Tot ACP activity was decreased in all treatments in caterpillars from polluted habitats and a new isoform band was detected on native gels, while in those from unpolluted forest, induced thermotolerance had the effect on the activity of tot ACP. Obtained results indicate the differences in sensitivity to increased environmental temperature between populations with different histories of exposure to pollution and that they must be considered as well.",
publisher = "Novi Sad: Faculty of Sciences, University of Novi Sad",
journal = "The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021",
title = "Sensitivity of midgut phosphatases to thermal stress in Gypsy moth (Lymantria Dispar) caterpillars",
pages = "64",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4784"
}

Ilijin, L., Grčić, A., Mrdaković, M., Vlahović, M., Matić, D., Todorović, D.,& Perić Mataruga, V.. (2021). Sensitivity of midgut phosphatases to thermal stress in Gypsy moth (Lymantria Dispar) caterpillars. in The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021
Novi Sad: Faculty of Sciences, University of Novi Sad., 64.
https://hdl.handle.net/21.15107/rcub_ibiss_4784

Ilijin L, Grčić A, Mrdaković M, Vlahović M, Matić D, Todorović D, Perić Mataruga V. Sensitivity of midgut phosphatases to thermal stress in Gypsy moth (Lymantria Dispar) caterpillars. in The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021. 2021;:64.
https://hdl.handle.net/21.15107/rcub_ibiss_4784 .

Ilijin, Larisa, Grčić, Anja, Mrdaković, Marija, Vlahović, Milena, Matić, Dragana, Todorović, Dajana, Perić Mataruga, Vesna, "Sensitivity of midgut phosphatases to thermal stress in Gypsy moth (Lymantria Dispar) caterpillars" in The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021 (2021):64,
https://hdl.handle.net/21.15107/rcub_ibiss_4784 .

TY  - JOUR
AU  - Grčić, Anja
AU  - Ilijin, Larisa
AU  - Matić, Dragana
AU  - Filipović, Aleksandra
AU  - Mrdaković, Marija
AU  - Todorović, Dajana
AU  - Perić Mataruga, Vesna
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4283
AB  - Accumulation of organic pollutants in the environment calls for sensing physiological parameters adequate to
indicate the presence of contaminants and their effects on ecosystems. Evidence points to the importance of
insect adaptations in their habitats for the assessment of sensitive biomarkers so we examined the influence of
origin and multigenerational adaptations of the Lymantria dispar larvae to chronic benzo[a]pyrene (B[a]P)
treatment under laboratory conditions. The main aim was to compare reactions of larvae from unpolluted and
polluted forests using alkaline phosphatase (ALP), acid phosphatase (ACP), and carboxylesterase (CE) specific
activities in the midgut, including electrophoretic isoform patterns; midgut expression levels of Hsp70, larval
development time (DT), and midgut mass (MM), after chronic exposure to 5 and 50 ng of B[a]P/g dry food
weight. The biomarker potential of these parameters regarding larval pre-exposure history to pollution was
estimated by principal component analysis (PCA). B[a]P treatment resulted in inhibition of ALP activity, a rise of
CE activity, and reduction of MM in larvae from the unpolluted forest, while the population from the polluted
forest showed significant elevation of Hsp70 expression in the midgut, prolonged DT, and reduction of MM. PCA
confirmed variations in responses of the selected parameters regarding population origin. The obtained results
provide insight into insect population variability concerning physiological responses to pollutants. It is indicative
that all investigated physiological parameters of L. dispar larvae showed origin-dependent responses to long-term
presence of B[a]P, which may be of great importance in ecotoxicological research.
PB  - Elsevier
T2  - Environmntal Pollution
T1  - Sensitivity of midgut physiological parameters of Lymantria dispar L. larvae to benzo[a]pyrene in populations with different multigeneration contact to environmental pollutants
VL  - 288
DO  - 10.1016/j.envpol.2021.117706
SP  - 117706
ER  - 

@article{
author = "Grčić, Anja and Ilijin, Larisa and Matić, Dragana and Filipović, Aleksandra and Mrdaković, Marija and Todorović, Dajana and Perić Mataruga, Vesna",
year = "2021",
abstract = "Accumulation of organic pollutants in the environment calls for sensing physiological parameters adequate to
indicate the presence of contaminants and their effects on ecosystems. Evidence points to the importance of
insect adaptations in their habitats for the assessment of sensitive biomarkers so we examined the influence of
origin and multigenerational adaptations of the Lymantria dispar larvae to chronic benzo[a]pyrene (B[a]P)
treatment under laboratory conditions. The main aim was to compare reactions of larvae from unpolluted and
polluted forests using alkaline phosphatase (ALP), acid phosphatase (ACP), and carboxylesterase (CE) specific
activities in the midgut, including electrophoretic isoform patterns; midgut expression levels of Hsp70, larval
development time (DT), and midgut mass (MM), after chronic exposure to 5 and 50 ng of B[a]P/g dry food
weight. The biomarker potential of these parameters regarding larval pre-exposure history to pollution was
estimated by principal component analysis (PCA). B[a]P treatment resulted in inhibition of ALP activity, a rise of
CE activity, and reduction of MM in larvae from the unpolluted forest, while the population from the polluted
forest showed significant elevation of Hsp70 expression in the midgut, prolonged DT, and reduction of MM. PCA
confirmed variations in responses of the selected parameters regarding population origin. The obtained results
provide insight into insect population variability concerning physiological responses to pollutants. It is indicative
that all investigated physiological parameters of L. dispar larvae showed origin-dependent responses to long-term
presence of B[a]P, which may be of great importance in ecotoxicological research.",
publisher = "Elsevier",
journal = "Environmntal Pollution",
title = "Sensitivity of midgut physiological parameters of Lymantria dispar L. larvae to benzo[a]pyrene in populations with different multigeneration contact to environmental pollutants",
volume = "288",
doi = "10.1016/j.envpol.2021.117706",
pages = "117706"
}

Grčić, A., Ilijin, L., Matić, D., Filipović, A., Mrdaković, M., Todorović, D.,& Perić Mataruga, V.. (2021). Sensitivity of midgut physiological parameters of Lymantria dispar L. larvae to benzo[a]pyrene in populations with different multigeneration contact to environmental pollutants. in Environmntal Pollution
Elsevier., 288, 117706.
https://doi.org/10.1016/j.envpol.2021.117706

Grčić A, Ilijin L, Matić D, Filipović A, Mrdaković M, Todorović D, Perić Mataruga V. Sensitivity of midgut physiological parameters of Lymantria dispar L. larvae to benzo[a]pyrene in populations with different multigeneration contact to environmental pollutants. in Environmntal Pollution. 2021;288:117706.
doi:10.1016/j.envpol.2021.117706 .

Grčić, Anja, Ilijin, Larisa, Matić, Dragana, Filipović, Aleksandra, Mrdaković, Marija, Todorović, Dajana, Perić Mataruga, Vesna, "Sensitivity of midgut physiological parameters of Lymantria dispar L. larvae to benzo[a]pyrene in populations with different multigeneration contact to environmental pollutants" in Environmntal Pollution, 288 (2021):117706,
https://doi.org/10.1016/j.envpol.2021.117706 . .

TY  - JOUR
AU  - Todorović, Dajana
AU  - Ilijin, Larisa
AU  - Mrdaković, Marija
AU  - Vlahović, Milena
AU  - Grčić, Anja
AU  - Petković, Branka
AU  - Perić Mataruga, Vesna
PY  - 2020
UR  - https://www.tandfonline.com/doi/abs/10.1080/09553002.2020.1770360
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32412321
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3759
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3821
AB  - Purpose: This study deals with a comparative analysis of the effects of chronic exposure to a static magnetic field (SMF) and an extremely low frequency magnetic field (ELF MF) in Blaptica dubia nymphs. The outcome of such treatment on insect and fat body mass, glycogen and total lipid content in the fat body and locomotion, as an energy demanding process, were examined. Materials and methods: One-month-old nymphs of B. dubia were exposed to an SMF (110 mT) or ELF MF (50 Hz, 10 mT) for 5 months. Their locomotion was monitored in the 'open-field' test for 10 min and expressed as travel distance, time in movement and average speed while in motion. After that, fat body mass and content of its main components (glycogen and total lipids) were determined. Nymph body mass was also estimated after 1 and 5 months of MF treatment. Results: Chronic exposure to the SMF and ELF MF decreased nymph body mass and glycogen content in the fat body but increased all examined parameters of locomotion. In addition, chronic SMF treatment elevated total lipid content in the fat body, while chronic ELF MF treatment reduced fat body mass and total lipid content. Conclusions: These findings indicate that B. dubia nymphs are sensitive to the applied MFs and possess different strategies for fuel usage in response to the SMF and ELF MF in order to satisfy increased energy demands and to overcome stressful conditions.
PB  - Taylor and Francis Ltd
T2  - International Journal of Radiation Biology
T1  - The impact of chronic exposure to a magnetic field on energy metabolism and locomotion of Blaptica dubia
IS  - 8
VL  - 96
DO  - 10.1080/09553002.2020.1770360
SP  - 1076
EP  - 1083
ER  - 

@article{
author = "Todorović, Dajana and Ilijin, Larisa and Mrdaković, Marija and Vlahović, Milena and Grčić, Anja and Petković, Branka and Perić Mataruga, Vesna",
year = "2020",
abstract = "Purpose: This study deals with a comparative analysis of the effects of chronic exposure to a static magnetic field (SMF) and an extremely low frequency magnetic field (ELF MF) in Blaptica dubia nymphs. The outcome of such treatment on insect and fat body mass, glycogen and total lipid content in the fat body and locomotion, as an energy demanding process, were examined. Materials and methods: One-month-old nymphs of B. dubia were exposed to an SMF (110 mT) or ELF MF (50 Hz, 10 mT) for 5 months. Their locomotion was monitored in the 'open-field' test for 10 min and expressed as travel distance, time in movement and average speed while in motion. After that, fat body mass and content of its main components (glycogen and total lipids) were determined. Nymph body mass was also estimated after 1 and 5 months of MF treatment. Results: Chronic exposure to the SMF and ELF MF decreased nymph body mass and glycogen content in the fat body but increased all examined parameters of locomotion. In addition, chronic SMF treatment elevated total lipid content in the fat body, while chronic ELF MF treatment reduced fat body mass and total lipid content. Conclusions: These findings indicate that B. dubia nymphs are sensitive to the applied MFs and possess different strategies for fuel usage in response to the SMF and ELF MF in order to satisfy increased energy demands and to overcome stressful conditions.",
publisher = "Taylor and Francis Ltd",
journal = "International Journal of Radiation Biology",
title = "The impact of chronic exposure to a magnetic field on energy metabolism and locomotion of Blaptica dubia",
number = "8",
volume = "96",
doi = "10.1080/09553002.2020.1770360",
pages = "1076-1083"
}

Todorović, D., Ilijin, L., Mrdaković, M., Vlahović, M., Grčić, A., Petković, B.,& Perić Mataruga, V.. (2020). The impact of chronic exposure to a magnetic field on energy metabolism and locomotion of Blaptica dubia. in International Journal of Radiation Biology
Taylor and Francis Ltd., 96(8), 1076-1083.
https://doi.org/10.1080/09553002.2020.1770360

Todorović D, Ilijin L, Mrdaković M, Vlahović M, Grčić A, Petković B, Perić Mataruga V. The impact of chronic exposure to a magnetic field on energy metabolism and locomotion of Blaptica dubia. in International Journal of Radiation Biology. 2020;96(8):1076-1083.
doi:10.1080/09553002.2020.1770360 .

Todorović, Dajana, Ilijin, Larisa, Mrdaković, Marija, Vlahović, Milena, Grčić, Anja, Petković, Branka, Perić Mataruga, Vesna, "The impact of chronic exposure to a magnetic field on energy metabolism and locomotion of Blaptica dubia" in International Journal of Radiation Biology, 96, no. 8 (2020):1076-1083,
https://doi.org/10.1080/09553002.2020.1770360 . .

TY  - JOUR
AU  - Todorović, Dajana
AU  - Ilijin, Larisa
AU  - Mrdaković, Marija
AU  - Vlahović, Milena
AU  - Grčić, Anja
AU  - Petković, Branka
AU  - Perić Mataruga, Vesna
PY  - 2020
UR  - https://www.tandfonline.com/doi/abs/10.1080/09553002.2020.1770360
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32412321
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3759
AB  - Purpose: This study deals with a comparative analysis of the effects of chronic exposure to a static magnetic field (SMF) and an extremely low frequency magnetic field (ELF MF) in Blaptica dubia nymphs. The outcome of such treatment on insect and fat body mass, glycogen and total lipid content in the fat body and locomotion, as an energy demanding process, were examined. Materials and methods: One-month-old nymphs of B. dubia were exposed to an SMF (110 mT) or ELF MF (50 Hz, 10 mT) for 5 months. Their locomotion was monitored in the 'open-field' test for 10 min and expressed as travel distance, time in movement and average speed while in motion. After that, fat body mass and content of its main components (glycogen and total lipids) were determined. Nymph body mass was also estimated after 1 and 5 months of MF treatment. Results: Chronic exposure to the SMF and ELF MF decreased nymph body mass and glycogen content in the fat body but increased all examined parameters of locomotion. In addition, chronic SMF treatment elevated total lipid content in the fat body, while chronic ELF MF treatment reduced fat body mass and total lipid content. Conclusions: These findings indicate that B. dubia nymphs are sensitive to the applied MFs and possess different strategies for fuel usage in response to the SMF and ELF MF in order to satisfy increased energy demands and to overcome stressful conditions.
PB  - Taylor and Francis Ltd
T2  - International Journal of Radiation Biology
T1  - The impact of chronic exposure to a magnetic field on energy metabolism and locomotion of Blaptica dubia
IS  - 8
VL  - 96
DO  - 10.1080/09553002.2020.1770360
SP  - 1076
EP  - 1083
ER  - 

@article{
author = "Todorović, Dajana and Ilijin, Larisa and Mrdaković, Marija and Vlahović, Milena and Grčić, Anja and Petković, Branka and Perić Mataruga, Vesna",
year = "2020",
abstract = "Purpose: This study deals with a comparative analysis of the effects of chronic exposure to a static magnetic field (SMF) and an extremely low frequency magnetic field (ELF MF) in Blaptica dubia nymphs. The outcome of such treatment on insect and fat body mass, glycogen and total lipid content in the fat body and locomotion, as an energy demanding process, were examined. Materials and methods: One-month-old nymphs of B. dubia were exposed to an SMF (110 mT) or ELF MF (50 Hz, 10 mT) for 5 months. Their locomotion was monitored in the 'open-field' test for 10 min and expressed as travel distance, time in movement and average speed while in motion. After that, fat body mass and content of its main components (glycogen and total lipids) were determined. Nymph body mass was also estimated after 1 and 5 months of MF treatment. Results: Chronic exposure to the SMF and ELF MF decreased nymph body mass and glycogen content in the fat body but increased all examined parameters of locomotion. In addition, chronic SMF treatment elevated total lipid content in the fat body, while chronic ELF MF treatment reduced fat body mass and total lipid content. Conclusions: These findings indicate that B. dubia nymphs are sensitive to the applied MFs and possess different strategies for fuel usage in response to the SMF and ELF MF in order to satisfy increased energy demands and to overcome stressful conditions.",
publisher = "Taylor and Francis Ltd",
journal = "International Journal of Radiation Biology",
title = "The impact of chronic exposure to a magnetic field on energy metabolism and locomotion of Blaptica dubia",
number = "8",
volume = "96",
doi = "10.1080/09553002.2020.1770360",
pages = "1076-1083"
}

Todorović, D., Ilijin, L., Mrdaković, M., Vlahović, M., Grčić, A., Petković, B.,& Perić Mataruga, V.. (2020). The impact of chronic exposure to a magnetic field on energy metabolism and locomotion of Blaptica dubia. in International Journal of Radiation Biology
Taylor and Francis Ltd., 96(8), 1076-1083.
https://doi.org/10.1080/09553002.2020.1770360

Todorović D, Ilijin L, Mrdaković M, Vlahović M, Grčić A, Petković B, Perić Mataruga V. The impact of chronic exposure to a magnetic field on energy metabolism and locomotion of Blaptica dubia. in International Journal of Radiation Biology. 2020;96(8):1076-1083.
doi:10.1080/09553002.2020.1770360 .

Todorović, Dajana, Ilijin, Larisa, Mrdaković, Marija, Vlahović, Milena, Grčić, Anja, Petković, Branka, Perić Mataruga, Vesna, "The impact of chronic exposure to a magnetic field on energy metabolism and locomotion of Blaptica dubia" in International Journal of Radiation Biology, 96, no. 8 (2020):1076-1083,
https://doi.org/10.1080/09553002.2020.1770360 . .

TY  - JOUR
AU  - Matić, Dragana
AU  - Vlahović, Milena
AU  - Ilijin, Larisa
AU  - Mrdaković, Marija
AU  - Grčić, Anja
AU  - Filipović, Aleksandra
AU  - Perić Mataruga, Vesna
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3606
AB  - Long-term exposure of invertebrate populations to pollution might result in adaptations that must be taken into account when evaluating physiological parameters as biomarkers of contamination. In the present study we investigated whether previous exposure of gypsy moth, Lymantria dispar L. (Lepidoptera), population to pollution affects the sensitivity of larvae to cadmium treatment under laboratory conditions. Our goals in the current study were to compare metallothionein (MT) level and non-specific esterase activity including isoform expression in the midgut and fitness-related traits between caterpillars originating from polluted and unpolluted localities after chronic oral intake (50 and 100 µg Cd/g dry food). Besides being individually assessed as biomarkers of cadmium contamination, the responses of above parameters related to the midgut (MT concentration, specific activity of esterases and midgut mass) were summarized into the integrated biomarker response (IBR). Upon cadmium exposure, MT concentration increased significantly only in larvae originating from the polluted site, whereas non-specific esterase activity decreased in caterpillars from the unpolluted forest. We noticed similar patterns of esterase isoforms in both populations, including isoform number three that appeared only after exposure to the lower cadmium concentration. Midgut mass was reduced only in the group from the unpolluted locality exposed to the higher cadmium concentration. IBR index increased in a concentration-dependent manner in both populations, reflecting higher sensitivity of larvae from the unpolluted locality to the metal. Neither cadmium exposure nor population origin influenced larval survival. While MT concentration could be applied as a biomarker of cadmium exposure in populations that have been previously exposed to pollution for a longer time, non-specific esterase activity could indicate metal presence at unpolluted locations. IBR index and expression of esterase isoform number three could be used universally, regardless of the exposure history, except the latter might be limited to lower cadmium concentrations.
T2  - Ecological Indicators
T1  - Metallothionein level, non-specific esterases, fitness-related traits and integrated biomarker response (IBR) in larvae of Lymantria dispar L. (Lepidoptera) originating from unpolluted and polluted locations after chronic cadmium treatment
VL  - 112
DO  - 10.1016/j.ecolind.2020.106136
SP  - 106136
ER  - 

@article{
author = "Matić, Dragana and Vlahović, Milena and Ilijin, Larisa and Mrdaković, Marija and Grčić, Anja and Filipović, Aleksandra and Perić Mataruga, Vesna",
year = "2020",
abstract = "Long-term exposure of invertebrate populations to pollution might result in adaptations that must be taken into account when evaluating physiological parameters as biomarkers of contamination. In the present study we investigated whether previous exposure of gypsy moth, Lymantria dispar L. (Lepidoptera), population to pollution affects the sensitivity of larvae to cadmium treatment under laboratory conditions. Our goals in the current study were to compare metallothionein (MT) level and non-specific esterase activity including isoform expression in the midgut and fitness-related traits between caterpillars originating from polluted and unpolluted localities after chronic oral intake (50 and 100 µg Cd/g dry food). Besides being individually assessed as biomarkers of cadmium contamination, the responses of above parameters related to the midgut (MT concentration, specific activity of esterases and midgut mass) were summarized into the integrated biomarker response (IBR). Upon cadmium exposure, MT concentration increased significantly only in larvae originating from the polluted site, whereas non-specific esterase activity decreased in caterpillars from the unpolluted forest. We noticed similar patterns of esterase isoforms in both populations, including isoform number three that appeared only after exposure to the lower cadmium concentration. Midgut mass was reduced only in the group from the unpolluted locality exposed to the higher cadmium concentration. IBR index increased in a concentration-dependent manner in both populations, reflecting higher sensitivity of larvae from the unpolluted locality to the metal. Neither cadmium exposure nor population origin influenced larval survival. While MT concentration could be applied as a biomarker of cadmium exposure in populations that have been previously exposed to pollution for a longer time, non-specific esterase activity could indicate metal presence at unpolluted locations. IBR index and expression of esterase isoform number three could be used universally, regardless of the exposure history, except the latter might be limited to lower cadmium concentrations.",
journal = "Ecological Indicators",
title = "Metallothionein level, non-specific esterases, fitness-related traits and integrated biomarker response (IBR) in larvae of Lymantria dispar L. (Lepidoptera) originating from unpolluted and polluted locations after chronic cadmium treatment",
volume = "112",
doi = "10.1016/j.ecolind.2020.106136",
pages = "106136"
}

Matić, D., Vlahović, M., Ilijin, L., Mrdaković, M., Grčić, A., Filipović, A.,& Perić Mataruga, V.. (2020). Metallothionein level, non-specific esterases, fitness-related traits and integrated biomarker response (IBR) in larvae of Lymantria dispar L. (Lepidoptera) originating from unpolluted and polluted locations after chronic cadmium treatment. in Ecological Indicators, 112, 106136.
https://doi.org/10.1016/j.ecolind.2020.106136

Matić D, Vlahović M, Ilijin L, Mrdaković M, Grčić A, Filipović A, Perić Mataruga V. Metallothionein level, non-specific esterases, fitness-related traits and integrated biomarker response (IBR) in larvae of Lymantria dispar L. (Lepidoptera) originating from unpolluted and polluted locations after chronic cadmium treatment. in Ecological Indicators. 2020;112:106136.
doi:10.1016/j.ecolind.2020.106136 .

Matić, Dragana, Vlahović, Milena, Ilijin, Larisa, Mrdaković, Marija, Grčić, Anja, Filipović, Aleksandra, Perić Mataruga, Vesna, "Metallothionein level, non-specific esterases, fitness-related traits and integrated biomarker response (IBR) in larvae of Lymantria dispar L. (Lepidoptera) originating from unpolluted and polluted locations after chronic cadmium treatment" in Ecological Indicators, 112 (2020):106136,
https://doi.org/10.1016/j.ecolind.2020.106136 . .

TY  - THES
AU  - Grčić, Anja
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3683
AB  - Intenzivan antropogeni uticaj u poslednjih nekoliko decenija doveo je do značajnog porasta stepena zagađenja biosfere, kojem u velikoj meri doprinosi toksični i kancerogeni organski polutant - policiklični aromatični ugljovodonik, benzo[a]piren. Cilj ove doktorske disertacije bio je procena odgovora fizioloških i molekularnih parametara larvi Lymantria dispar kao potencijalnih biomarkera sredinskog zagađenja benzo[a]pirenom. Ispitan je hronični uticaj dve koncentracije ovog ksenobiotika (5 i 50 ng/g suve mase hrane) na larve L. dispar iz legala sakupljenih u nezagađenoj šumi (planina Majdanpek) i sakupljenih u zagađenoj šumi (okolina rudnika Bor D.O.O), sa svrhom procene efekta porekla populacije. Larve iz nezagađene šume pokazuju pad specifične aktivnosti katalaze u srednjem crevu, ali i značajan porast aktivnosti antioksidativnih enzima - superoksid dismutaze i katalaze, na nivou hemolimfe, dok je u populaciji iz zagađene šume zabeleženo povećanje aktivnosti ova dva enzima samo u okviru srednjeg creva. Detoksifikacioni enzimi, karboksilesteraze, pokazuju osetljivost i povećanje aktivnosti u prisustvu benzo[a]pirena, u srednjem crevu larvi iz nezagađene šume, kao i u mozgu larvi iz zagađene šume, koje pored toga, na nivou srednjeg creva odlikuje značajna indukcija aktivnosti glutation S-transferaza. Acetilholinesteraze nisu osetljive na primenjene koncentracije ovog ksenobiotika. Povećanje ekspresije proteina Hsp70 u okviru srednjeg creva uočeno je kod obe populacije larvi, a na nivou mozga jedino u populaciji iz zagađene šume i to pri dejstvu niže koncentracije benzo[a]pirena. Većina digestivnih enzima nije osetljiva na prisustvo benzo[a]pirena sa izuzetkom tripsina, alkalnih fosfataza i lipaza, koje odlikuje povećanje aktivnosti kod larvi iz nezagađene šume, a larve iz zagađene šume karakteriše jedino indukcija lipazne aktivnosti. Za populaciju larvi iz nezagađene šume svojstven je pad koncentracije ukupnih lipida u hemolimfi i nepromenjen sadržaj trehaloze, dok ovi parametri ne pokazuju promene u populaciji iz zagađene šume. Hronično prisustvo benzo[a]pirena i produženo angažovanje energetski zahtevnih, odbrambenih mehanizama, odrazilo se na komponente fitnesa larvi L. dispar pri čemu se populacija iz nezagađene šume pokazala manje podložnom energetskom deficitu, ispoljavajući smanjenje mase i relativne brzine rasta larvi petog larvenog stupnja, dok larve iz zagađene šume, pored iste promene prethodna dva parametra fitnesa, odlikuje i produženo razviće. Uočeno je da su odgovori antioksidativnih enzima - superoksid dismutaze i katalaze, u hemolimfi, kao i karboksilesteraza i digestivnih enzima - tripsina i alkalnih fosfataza, u srednjem crevu larvi, specifični i potencijalno primenjivi biomarkeri zagađenosti životne sredine sa benzo[a]pirenom za populaciju larvi poreklom iz nezagađene šume. Sličnu primenu, kada je u pitanju populacija larvi iz zagađene šume, mogu naći specifične aktivnosti superoksid dismutaze, katalaza i glutation S-transferaza u srednjem crevu larvi, aktivnost karboksilesteraza i ekspresija Hsp70 u mozgu larvi L. dispar.
AB  - Over the last few decades intense anthropogenic influence has led to a significant increase of the pollution levels in the biosphere, which can greatly be attributed to the toxic and carcinogenic organic pollutants - polycyclic aromatic hydrocarbones, and especially to the benzo[a]pyrene. The aim of this dissertation was to evaluate responses of the physiological and molecular parameters of the Lymantria dispar larvae as a potential biomarkers of environmental pollution by benzo[a]pyrene. For the purpose of assessing the effects of population origin, L. dispar egg-masses were collected from unpolluted forest (Majdanpek mountain) and polluted forest (vicinity of Bor D.O.O mine). The chronic effects of the two benzo[a]pyrene concentrations in the diet (5 and 50 ng/g dry food weight) on these two population of larvae were investigated. Larvae from unpolluted forest showed a decrease of the catalase specific activity in the midgut, and a significant increase in the activity of antioxidative enzymes - superoxide dismutase and catalase, in the hemolymph, while the population from the polluted forest expressed an increase in the activity of these two enzymes only within the midgut. Detoxification enzymes - carboxylesterases, showed sensitivity and increase in activity in the presence of benzo[a]pyrene in the midgut of larvae from unpolluted forest, as well as in the brain of larvae from polluted forest, which in addition, are characterized by significant induction of glutathione S-transferase activity at the midgut level. Acetylcholinesterases are non inducible to the applied concentrations of this xenobiotic. An increase in expression of Hsp70 protein at midgut level was observed in both larval populations, while only the population from contaminated forest showed rise in brain Hsp70 concentration, after treatment with lower dose of benzo[a]pyrene. Most digestive enzymes are insensitive to the presence of benzo[a]pyrene with the exception of trypsin, alkaline phosphatases, and lipases which demonstrated increased activity in larvae from unpolluted forest. Larvae from polluted forest exibited only induction of lipase activity. The population of non-polluted larvae is characterized by a decrease in the concentration of total lipids in the hemolymph and an unchanged trehalose content, while these parameters do not show changes in the population from the contaminated forest. The chronic presence of benzo[a]pyrene and prolonged engagement of energy-demanding defense mechanisms have reflected on the fitness components of L. dispar larvae, with population from unpolluted forest showing less susceptibility to energy deficiency and exhibiting reduced mass and relative growth rates of the fifth instar larvae, while the larvae from the polluted forest, in addition to the same changes in previous two fitness parameters, have also showed prolonged development. It have been noticed that responses of the antioxidative enzymes - superoxide dismutase and catalase in hemolymph, as well as carboxylesterases and digestive enzymes - trypsin and alkaline phosphatases, from the larvae midgut, are specific and potentially applicable biomarkers of environmental contamination with benzo[a]pyrene for larval populations originally from unpolluted forest systems. Similarly, when it comes to larval populations from contaminated forests, specific activities of superoxide dismutase, catalase and glutathione S-transferases in the larvae midgut, as well as carboxylesterase activity and expression of Hsp70 in the brain of L. dispar larvae, could be potentially used in biomonitoring.
PB  - Belgrade: University of Belgrade, Faculty of Biology
T2  - University of Belgrade, Faculty of Biology
T1  - Bioindikatorski potencijal fizioloških odgovora gubara Lymantria dispar L. (Lepidoptera) na delovanje benzo[a]pirena
T1  - Bioindication potential of physiological responses of gypsy moth Lymantria dispar L. (Lepidoptera) on the benzo[a]pyren effects
SP  - 1
EP  - 113
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3683
ER  - 

@phdthesis{
author = "Grčić, Anja",
year = "2020",
abstract = "Intenzivan antropogeni uticaj u poslednjih nekoliko decenija doveo je do značajnog porasta stepena zagađenja biosfere, kojem u velikoj meri doprinosi toksični i kancerogeni organski polutant - policiklični aromatični ugljovodonik, benzo[a]piren. Cilj ove doktorske disertacije bio je procena odgovora fizioloških i molekularnih parametara larvi Lymantria dispar kao potencijalnih biomarkera sredinskog zagađenja benzo[a]pirenom. Ispitan je hronični uticaj dve koncentracije ovog ksenobiotika (5 i 50 ng/g suve mase hrane) na larve L. dispar iz legala sakupljenih u nezagađenoj šumi (planina Majdanpek) i sakupljenih u zagađenoj šumi (okolina rudnika Bor D.O.O), sa svrhom procene efekta porekla populacije. Larve iz nezagađene šume pokazuju pad specifične aktivnosti katalaze u srednjem crevu, ali i značajan porast aktivnosti antioksidativnih enzima - superoksid dismutaze i katalaze, na nivou hemolimfe, dok je u populaciji iz zagađene šume zabeleženo povećanje aktivnosti ova dva enzima samo u okviru srednjeg creva. Detoksifikacioni enzimi, karboksilesteraze, pokazuju osetljivost i povećanje aktivnosti u prisustvu benzo[a]pirena, u srednjem crevu larvi iz nezagađene šume, kao i u mozgu larvi iz zagađene šume, koje pored toga, na nivou srednjeg creva odlikuje značajna indukcija aktivnosti glutation S-transferaza. Acetilholinesteraze nisu osetljive na primenjene koncentracije ovog ksenobiotika. Povećanje ekspresije proteina Hsp70 u okviru srednjeg creva uočeno je kod obe populacije larvi, a na nivou mozga jedino u populaciji iz zagađene šume i to pri dejstvu niže koncentracije benzo[a]pirena. Većina digestivnih enzima nije osetljiva na prisustvo benzo[a]pirena sa izuzetkom tripsina, alkalnih fosfataza i lipaza, koje odlikuje povećanje aktivnosti kod larvi iz nezagađene šume, a larve iz zagađene šume karakteriše jedino indukcija lipazne aktivnosti. Za populaciju larvi iz nezagađene šume svojstven je pad koncentracije ukupnih lipida u hemolimfi i nepromenjen sadržaj trehaloze, dok ovi parametri ne pokazuju promene u populaciji iz zagađene šume. Hronično prisustvo benzo[a]pirena i produženo angažovanje energetski zahtevnih, odbrambenih mehanizama, odrazilo se na komponente fitnesa larvi L. dispar pri čemu se populacija iz nezagađene šume pokazala manje podložnom energetskom deficitu, ispoljavajući smanjenje mase i relativne brzine rasta larvi petog larvenog stupnja, dok larve iz zagađene šume, pored iste promene prethodna dva parametra fitnesa, odlikuje i produženo razviće. Uočeno je da su odgovori antioksidativnih enzima - superoksid dismutaze i katalaze, u hemolimfi, kao i karboksilesteraza i digestivnih enzima - tripsina i alkalnih fosfataza, u srednjem crevu larvi, specifični i potencijalno primenjivi biomarkeri zagađenosti životne sredine sa benzo[a]pirenom za populaciju larvi poreklom iz nezagađene šume. Sličnu primenu, kada je u pitanju populacija larvi iz zagađene šume, mogu naći specifične aktivnosti superoksid dismutaze, katalaza i glutation S-transferaza u srednjem crevu larvi, aktivnost karboksilesteraza i ekspresija Hsp70 u mozgu larvi L. dispar., Over the last few decades intense anthropogenic influence has led to a significant increase of the pollution levels in the biosphere, which can greatly be attributed to the toxic and carcinogenic organic pollutants - polycyclic aromatic hydrocarbones, and especially to the benzo[a]pyrene. The aim of this dissertation was to evaluate responses of the physiological and molecular parameters of the Lymantria dispar larvae as a potential biomarkers of environmental pollution by benzo[a]pyrene. For the purpose of assessing the effects of population origin, L. dispar egg-masses were collected from unpolluted forest (Majdanpek mountain) and polluted forest (vicinity of Bor D.O.O mine). The chronic effects of the two benzo[a]pyrene concentrations in the diet (5 and 50 ng/g dry food weight) on these two population of larvae were investigated. Larvae from unpolluted forest showed a decrease of the catalase specific activity in the midgut, and a significant increase in the activity of antioxidative enzymes - superoxide dismutase and catalase, in the hemolymph, while the population from the polluted forest expressed an increase in the activity of these two enzymes only within the midgut. Detoxification enzymes - carboxylesterases, showed sensitivity and increase in activity in the presence of benzo[a]pyrene in the midgut of larvae from unpolluted forest, as well as in the brain of larvae from polluted forest, which in addition, are characterized by significant induction of glutathione S-transferase activity at the midgut level. Acetylcholinesterases are non inducible to the applied concentrations of this xenobiotic. An increase in expression of Hsp70 protein at midgut level was observed in both larval populations, while only the population from contaminated forest showed rise in brain Hsp70 concentration, after treatment with lower dose of benzo[a]pyrene. Most digestive enzymes are insensitive to the presence of benzo[a]pyrene with the exception of trypsin, alkaline phosphatases, and lipases which demonstrated increased activity in larvae from unpolluted forest. Larvae from polluted forest exibited only induction of lipase activity. The population of non-polluted larvae is characterized by a decrease in the concentration of total lipids in the hemolymph and an unchanged trehalose content, while these parameters do not show changes in the population from the contaminated forest. The chronic presence of benzo[a]pyrene and prolonged engagement of energy-demanding defense mechanisms have reflected on the fitness components of L. dispar larvae, with population from unpolluted forest showing less susceptibility to energy deficiency and exhibiting reduced mass and relative growth rates of the fifth instar larvae, while the larvae from the polluted forest, in addition to the same changes in previous two fitness parameters, have also showed prolonged development. It have been noticed that responses of the antioxidative enzymes - superoxide dismutase and catalase in hemolymph, as well as carboxylesterases and digestive enzymes - trypsin and alkaline phosphatases, from the larvae midgut, are specific and potentially applicable biomarkers of environmental contamination with benzo[a]pyrene for larval populations originally from unpolluted forest systems. Similarly, when it comes to larval populations from contaminated forests, specific activities of superoxide dismutase, catalase and glutathione S-transferases in the larvae midgut, as well as carboxylesterase activity and expression of Hsp70 in the brain of L. dispar larvae, could be potentially used in biomonitoring.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "University of Belgrade, Faculty of Biology",
title = "Bioindikatorski potencijal fizioloških odgovora gubara Lymantria dispar L. (Lepidoptera) na delovanje benzo[a]pirena, Bioindication potential of physiological responses of gypsy moth Lymantria dispar L. (Lepidoptera) on the benzo[a]pyren effects",
pages = "1-113",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3683"
}

Grčić, A.. (2020). Bioindikatorski potencijal fizioloških odgovora gubara Lymantria dispar L. (Lepidoptera) na delovanje benzo[a]pirena. in University of Belgrade, Faculty of Biology
Belgrade: University of Belgrade, Faculty of Biology., 1-113.
https://hdl.handle.net/21.15107/rcub_ibiss_3683

Grčić A. Bioindikatorski potencijal fizioloških odgovora gubara Lymantria dispar L. (Lepidoptera) na delovanje benzo[a]pirena. in University of Belgrade, Faculty of Biology. 2020;:1-113.
https://hdl.handle.net/21.15107/rcub_ibiss_3683 .

Grčić, Anja, "Bioindikatorski potencijal fizioloških odgovora gubara Lymantria dispar L. (Lepidoptera) na delovanje benzo[a]pirena" in University of Belgrade, Faculty of Biology (2020):1-113,
https://hdl.handle.net/21.15107/rcub_ibiss_3683 .

TY  - JOUR
AU  - Filipović, Aleksandra
AU  - Mrdaković, Marija
AU  - Ilijin, Larisa
AU  - Vlahović, Milena
AU  - Todorović, Dajana
AU  - Grčić, Anja
AU  - Perić Mataruga, Vesna
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S1532045619302753?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3436
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3437
AB  - This study examined the effect of long-term exposure to environmentally relevant concentrations of dietary fluoranthene (6.7 and 67 ng / g dry food weight) on defense mechanisms of the polyphagous forest insects Lymantria dispar L. and Euproctis chrysorrhoea L. The activities and expression of isoforms of superoxide dismutase (SOD) and catalase (CAT), the activities of glutathione S-transferase (GST) and glutathione reductase (GR), and total glutathione content (GSH) were determined in the whole midgut and midgut tissue, while SOD and CAT activities were assessed in hemolymph of the larvae. The results showed significant changes of enzyme activities, with more pronounced responses in larval midgut tissues, and between-species differences in patterns of response. Significantly increased activity of SOD was recorded in the whole midgut and midgut tissue of L. dispar larvae, as well as in midgut tissue of E. chrysorrhoea larvae. Fluoranthene increased CAT activity in midgut tissue of L. dispar larvae, and in the whole midgut and midgut tissue of E. chrysorrhoea larvae. Different expression patterns were detected for enzyme isoforms in tissues of larvae exposed to dietary fluoranthene. Total GSH content and GST activity increased in E. chrysorrhoea larval midgut tissue. Significantly decreased SOD activity in hemolymph of L. dispar larvae, and opposite changes in CAT activity were recorded in the hemolymph of larvae of two insect species. The tissue-specific responses of enzymes to dietary fluoranthene, recorded in each species, enabled the larvae to overcome the pollutant induced oxidative stress, and suggest further assessment of their possible use as early-warning signals of environmental pollution.
PB  - Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Effect of fluoranthene on antioxidative defense in different tissues of Lymantria dispar and Euproctis chrysorrhoea larvae.
VL  - 224
DO  - 10.1016/j.cbpc.2019.108565
SP  - 108565
ER  - 

@article{
author = "Filipović, Aleksandra and Mrdaković, Marija and Ilijin, Larisa and Vlahović, Milena and Todorović, Dajana and Grčić, Anja and Perić Mataruga, Vesna",
year = "2019",
abstract = "This study examined the effect of long-term exposure to environmentally relevant concentrations of dietary fluoranthene (6.7 and 67 ng / g dry food weight) on defense mechanisms of the polyphagous forest insects Lymantria dispar L. and Euproctis chrysorrhoea L. The activities and expression of isoforms of superoxide dismutase (SOD) and catalase (CAT), the activities of glutathione S-transferase (GST) and glutathione reductase (GR), and total glutathione content (GSH) were determined in the whole midgut and midgut tissue, while SOD and CAT activities were assessed in hemolymph of the larvae. The results showed significant changes of enzyme activities, with more pronounced responses in larval midgut tissues, and between-species differences in patterns of response. Significantly increased activity of SOD was recorded in the whole midgut and midgut tissue of L. dispar larvae, as well as in midgut tissue of E. chrysorrhoea larvae. Fluoranthene increased CAT activity in midgut tissue of L. dispar larvae, and in the whole midgut and midgut tissue of E. chrysorrhoea larvae. Different expression patterns were detected for enzyme isoforms in tissues of larvae exposed to dietary fluoranthene. Total GSH content and GST activity increased in E. chrysorrhoea larval midgut tissue. Significantly decreased SOD activity in hemolymph of L. dispar larvae, and opposite changes in CAT activity were recorded in the hemolymph of larvae of two insect species. The tissue-specific responses of enzymes to dietary fluoranthene, recorded in each species, enabled the larvae to overcome the pollutant induced oxidative stress, and suggest further assessment of their possible use as early-warning signals of environmental pollution.",
publisher = "Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Effect of fluoranthene on antioxidative defense in different tissues of Lymantria dispar and Euproctis chrysorrhoea larvae.",
volume = "224",
doi = "10.1016/j.cbpc.2019.108565",
pages = "108565"
}

Filipović, A., Mrdaković, M., Ilijin, L., Vlahović, M., Todorović, D., Grčić, A.,& Perić Mataruga, V.. (2019). Effect of fluoranthene on antioxidative defense in different tissues of Lymantria dispar and Euproctis chrysorrhoea larvae.. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
Elsevier Inc.., 224, 108565.
https://doi.org/10.1016/j.cbpc.2019.108565

Filipović A, Mrdaković M, Ilijin L, Vlahović M, Todorović D, Grčić A, Perić Mataruga V. Effect of fluoranthene on antioxidative defense in different tissues of Lymantria dispar and Euproctis chrysorrhoea larvae.. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2019;224:108565.
doi:10.1016/j.cbpc.2019.108565 .

Filipović, Aleksandra, Mrdaković, Marija, Ilijin, Larisa, Vlahović, Milena, Todorović, Dajana, Grčić, Anja, Perić Mataruga, Vesna, "Effect of fluoranthene on antioxidative defense in different tissues of Lymantria dispar and Euproctis chrysorrhoea larvae." in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 224 (2019):108565,
https://doi.org/10.1016/j.cbpc.2019.108565 . .

TY  - CONF
AU  - Petković, Branka
AU  - Ilijin, Larisa
AU  - Todorović, Dajana
AU  - Mrdaković, Marija
AU  - Vlahović, Milena
AU  - Grčić, Anja
AU  - Perić Mataruga, Vesna
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4021
AB  - During the evolution, all organisms are exposed to the constant action of different environmental
factors, biotic and abiotic, which are variable in time and space. Life on Earth is formed in a natural
magnetic field (geomagnetic field). Therefore, magnetic field (MF) is classified as abiotic factor that
help maintain different life processes of plants, animals and humans, as well as their biological and
physiological functions. Organisms are exposed to new type of artificial MFs, due to the increasing
electrification and technological development. So, MF is growing environmental pollutant and as a
consequence of response and/or adaptation of organisms on new, stressful conditions, changes could
be observed on all levels of the biological organization. So, the aim of this study was to evaluate the
long – term effect of a static MF (SMF) and an extremely low frequency MF (ELF MF) on the motor
behaviour in Blaptica dubia nymphs, as well as on their fat body glycogen concentration and total
lipid content. One month old nymphs were randomly divided into three experimental groups:
control, exposed to SMF (110 mT) and exposed to ELF MF (50 Hz, 10 mT). The cockroaches were
exposed to these MFs for 5 months, while the control ones were kept outside the reach of the
magnetic field. Fat body glycogen concentration, as well as total lipid content, was measured
spectrophotometrically according to Wyatt and Kalf (1957) and Stone and Mordue (1980),
respectively. Also, we monitored nymphal behaviour for 10 minutes in “open – field” test and
analyzed several behavioural parameters (travel distance, average speed while in motion, time
mobile, travel distance of the head, number of body rotations, immobility time) using ANY – maze
software. Exposure to SMF and ELF MF affected the all examined behavioural parameters. Namely,
in comparison to control cockroaches, all parameters, except immobility time, were significantly
higher in B. dubia nymphs exposed to both MFs. The difference between the MF groups was no
statistically significant. In SMF and ELF MF groups, fat body glycogen concentration was
significantly lower compared to control group. Between the MF groups significant differences were
not observed. The lipid concentration in the fatty body of B. dubia nymphs depended on the applied
MF. In nymphs exposed to SMF, total lipid content was significantly higher compared to control. ELF
MF caused its decrease compared to control and SMF group. These differences were significant only
for SMF group. This study provides evidences that long – term exposure of B. dubia nymphs to
magnetic fields induces important alterations in their motor behaviour, and consequently examined
parameters of carbohydrate and lipid metabolism.
PB  - Niš, Serbia: RAD Centre
C3  - Seventh International Conference on Radiation in Various Fields of research (RAD 2019)
T1  - Motor behaviour and energy metabolism of Blaptica dubia in artificial magnetic fields
SP  - 77
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4021
ER  - 

@conference{
author = "Petković, Branka and Ilijin, Larisa and Todorović, Dajana and Mrdaković, Marija and Vlahović, Milena and Grčić, Anja and Perić Mataruga, Vesna",
year = "2019",
abstract = "During the evolution, all organisms are exposed to the constant action of different environmental
factors, biotic and abiotic, which are variable in time and space. Life on Earth is formed in a natural
magnetic field (geomagnetic field). Therefore, magnetic field (MF) is classified as abiotic factor that
help maintain different life processes of plants, animals and humans, as well as their biological and
physiological functions. Organisms are exposed to new type of artificial MFs, due to the increasing
electrification and technological development. So, MF is growing environmental pollutant and as a
consequence of response and/or adaptation of organisms on new, stressful conditions, changes could
be observed on all levels of the biological organization. So, the aim of this study was to evaluate the
long – term effect of a static MF (SMF) and an extremely low frequency MF (ELF MF) on the motor
behaviour in Blaptica dubia nymphs, as well as on their fat body glycogen concentration and total
lipid content. One month old nymphs were randomly divided into three experimental groups:
control, exposed to SMF (110 mT) and exposed to ELF MF (50 Hz, 10 mT). The cockroaches were
exposed to these MFs for 5 months, while the control ones were kept outside the reach of the
magnetic field. Fat body glycogen concentration, as well as total lipid content, was measured
spectrophotometrically according to Wyatt and Kalf (1957) and Stone and Mordue (1980),
respectively. Also, we monitored nymphal behaviour for 10 minutes in “open – field” test and
analyzed several behavioural parameters (travel distance, average speed while in motion, time
mobile, travel distance of the head, number of body rotations, immobility time) using ANY – maze
software. Exposure to SMF and ELF MF affected the all examined behavioural parameters. Namely,
in comparison to control cockroaches, all parameters, except immobility time, were significantly
higher in B. dubia nymphs exposed to both MFs. The difference between the MF groups was no
statistically significant. In SMF and ELF MF groups, fat body glycogen concentration was
significantly lower compared to control group. Between the MF groups significant differences were
not observed. The lipid concentration in the fatty body of B. dubia nymphs depended on the applied
MF. In nymphs exposed to SMF, total lipid content was significantly higher compared to control. ELF
MF caused its decrease compared to control and SMF group. These differences were significant only
for SMF group. This study provides evidences that long – term exposure of B. dubia nymphs to
magnetic fields induces important alterations in their motor behaviour, and consequently examined
parameters of carbohydrate and lipid metabolism.",
publisher = "Niš, Serbia: RAD Centre",
journal = "Seventh International Conference on Radiation in Various Fields of research (RAD 2019)",
title = "Motor behaviour and energy metabolism of Blaptica dubia in artificial magnetic fields",
pages = "77",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4021"
}

Petković, B., Ilijin, L., Todorović, D., Mrdaković, M., Vlahović, M., Grčić, A.,& Perić Mataruga, V.. (2019). Motor behaviour and energy metabolism of Blaptica dubia in artificial magnetic fields. in Seventh International Conference on Radiation in Various Fields of research (RAD 2019)
Niš, Serbia: RAD Centre., 77.
https://hdl.handle.net/21.15107/rcub_ibiss_4021

Petković B, Ilijin L, Todorović D, Mrdaković M, Vlahović M, Grčić A, Perić Mataruga V. Motor behaviour and energy metabolism of Blaptica dubia in artificial magnetic fields. in Seventh International Conference on Radiation in Various Fields of research (RAD 2019). 2019;:77.
https://hdl.handle.net/21.15107/rcub_ibiss_4021 .

Petković, Branka, Ilijin, Larisa, Todorović, Dajana, Mrdaković, Marija, Vlahović, Milena, Grčić, Anja, Perić Mataruga, Vesna, "Motor behaviour and energy metabolism of Blaptica dubia in artificial magnetic fields" in Seventh International Conference on Radiation in Various Fields of research (RAD 2019) (2019):77,
https://hdl.handle.net/21.15107/rcub_ibiss_4021 .

TY  - JOUR
AU  - Grčić, Anja
AU  - Ilijin, Larisa
AU  - Mrdaković, Marija
AU  - Vlahović, Milena
AU  - Filipović, Aleksandra
AU  - Đurašević, Siniša
AU  - Perić Mataruga, Vesna
PY  - 2019
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/4403
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3647
AB  - Plant vegetation accumulates polycyclic aromatic hydrocarbons (PAHs) among which benzo[a]pyrene (B[a]P) is recognized as being very toxic, including cancerogenic. Lymantria dispar L. larvae are sensitive to changes in the environment, providing potential signs of pollutant presence. We examined the chronic effects of two concentrations of B[a] P on the activity of carboxylesterase (CaE), acetylcholinesterase (AChE) and heat shock protein 70 (Hsp70) levels in the brain tissue of two populations of L. dispar larvae, originating from unpolluted and polluted habitats. We found that the relative growth rate was significantly lower in both populations and that only larvae from polluted forests were sensitive to low B[a]P concentrations, exhibiting a significant increase in brain tissue CaE activity and Hsp70 concentration. AChE activity showed no changes in response to B[a]P exposure in either population. Examined biochemical parameters indicate that their sensitivity to chronic treatment with B[a]P was highly dependent on the pre-exposure history of L. dispar larvae, suggesting that they could be promising biomarkers of B[a]P and PAH pollution in forest ecosystems
PB  - Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Benzo[a]pyrene-induced changes in carboxylesterase, acetylcholinesterase and heat shock protein 70 of Lymantria dispar (Lepidoptera: Lymantriidae) from unpolluted and polluted forests
IS  - 4
VL  - 71
DO  - 10.2298/abs190620056g
SP  - 735
EP  - 745
ER  - 

@article{
author = "Grčić, Anja and Ilijin, Larisa and Mrdaković, Marija and Vlahović, Milena and Filipović, Aleksandra and Đurašević, Siniša and Perić Mataruga, Vesna",
year = "2019",
abstract = "Plant vegetation accumulates polycyclic aromatic hydrocarbons (PAHs) among which benzo[a]pyrene (B[a]P) is recognized as being very toxic, including cancerogenic. Lymantria dispar L. larvae are sensitive to changes in the environment, providing potential signs of pollutant presence. We examined the chronic effects of two concentrations of B[a] P on the activity of carboxylesterase (CaE), acetylcholinesterase (AChE) and heat shock protein 70 (Hsp70) levels in the brain tissue of two populations of L. dispar larvae, originating from unpolluted and polluted habitats. We found that the relative growth rate was significantly lower in both populations and that only larvae from polluted forests were sensitive to low B[a]P concentrations, exhibiting a significant increase in brain tissue CaE activity and Hsp70 concentration. AChE activity showed no changes in response to B[a]P exposure in either population. Examined biochemical parameters indicate that their sensitivity to chronic treatment with B[a]P was highly dependent on the pre-exposure history of L. dispar larvae, suggesting that they could be promising biomarkers of B[a]P and PAH pollution in forest ecosystems",
publisher = "Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Benzo[a]pyrene-induced changes in carboxylesterase, acetylcholinesterase and heat shock protein 70 of Lymantria dispar (Lepidoptera: Lymantriidae) from unpolluted and polluted forests",
number = "4",
volume = "71",
doi = "10.2298/abs190620056g",
pages = "735-745"
}

Grčić, A., Ilijin, L., Mrdaković, M., Vlahović, M., Filipović, A., Đurašević, S.,& Perić Mataruga, V.. (2019). Benzo[a]pyrene-induced changes in carboxylesterase, acetylcholinesterase and heat shock protein 70 of Lymantria dispar (Lepidoptera: Lymantriidae) from unpolluted and polluted forests. in Archives of Biological Sciences
Serbian Biological Society., 71(4), 735-745.
https://doi.org/10.2298/abs190620056g

Grčić A, Ilijin L, Mrdaković M, Vlahović M, Filipović A, Đurašević S, Perić Mataruga V. Benzo[a]pyrene-induced changes in carboxylesterase, acetylcholinesterase and heat shock protein 70 of Lymantria dispar (Lepidoptera: Lymantriidae) from unpolluted and polluted forests. in Archives of Biological Sciences. 2019;71(4):735-745.
doi:10.2298/abs190620056g .

Grčić, Anja, Ilijin, Larisa, Mrdaković, Marija, Vlahović, Milena, Filipović, Aleksandra, Đurašević, Siniša, Perić Mataruga, Vesna, "Benzo[a]pyrene-induced changes in carboxylesterase, acetylcholinesterase and heat shock protein 70 of Lymantria dispar (Lepidoptera: Lymantriidae) from unpolluted and polluted forests" in Archives of Biological Sciences, 71, no. 4 (2019):735-745,
https://doi.org/10.2298/abs190620056g . .