@conference{
author = "Banjanac, Tijana and Gašić, Uroš and Nestorović Živković, Jasmina and Dmitrović, Slavica and Aničić, Neda and Milutinović, Milica and Skorić, Marijana and Božunović, Jelena and Petrović, Luka and Šiler, Branislav and Filipović, Biljana and Matekalo, Dragana and Todorović, Miloš and Lukić, Tamara and Dragićević, Milan and Mišić, Danijela",
year = "2022",
abstract = "Nepeta species (fam. Lamiace, subfam. Nepetoideae, tribe Nepetae) are well known for their
pharmacological properties, biopesticide potential and behavioral effects on cats. Different biological activities of Nepeta species are closely related to their secondary metabolites, among which
iridoids predominate. Research objective of this study was to determine profiles of methanol-soluble iridoids in leaves of greenhouse-grown plants of 10 phylodiverse Nepeta species: N. grandiflora
M. Bieb., N. laevigata (D. Don) Hand.-Mazz., N. nervosa Royle ex Benth., N. stewartiana Diels, N. nuda
L., N. parnassica Heldr. & Sart., N. rtanjensis Diklić & Milojević, N. ernesti-mayeri Diklić & V. Nikolić, N.
cataria L., and N. sibirica L. Untargeted metabolomics approach adopting UHPLC/LTQ Orbitrap MSn
analysis in a negative ionization mode revealed the presence of totally 15 iridoids in samples, of
which 13 were glucosides and two were aglycons of iridoids. Iridoid compounds were identified
based on their monoisotopic masses, MSn
fragmentation, and previously reported MS data. The
results indicated that the iridoid profiles of the investigated species are very diverse both in terms
of qualitative content of the compounds and their relative abundance in extracts. Among analysed Nepeta species, Balkan Peninsula endemics (N. rtanjensis, N. parnassica, and N. ernesti-mayeri)
and N. cataria, are especially rich in isomers of 1,5,9-epideoxyloganic acid. 5-Deoxylamiol, nepetariaside, nepetanudoside B, and ajugol were detected in majority of analyzed species. The UHPLC-LTQ OrbiTrap MS analysis, in combination with chemometric data processing, has proven to
be an efficient tool for chemical differentiation between Nepeta taxa, and could aid the efforts to
unravel the chemical evolution within the genus.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Liquid chromatography with multistage mass spectrometry as a method for chemical differentiation of ten Nepeta species (fam. Lamiaceae)",
pages = "151",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5576"
}