TY  - JOUR
AU  - Milošević, Verica
AU  - Todorović, Danijela
AU  - Šošić-Jurjević, Branka 
AU  - Jarić, Ivana
AU  - Živanović, Jasmina
AU  - Ušćebrka, Gordana
AU  - Ajdžanović, Vladimir
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2302
AB  - The effects of estradiol (EDP) and human chorionic gonadotropin (HCG) on
   histological and stereological features of adrenocorticotropic (ACTH)
   cells in peripubertal female rats were examined. The first group of
   females received five injections of EDP (0.25 mg/kg b.w.), every second
   day from the 4th to 14th day after birth, and was killed at the
   peripubertal stage. The second group of females was given two injections
   of pregnyl-gonadotrophinum chorionicum (HCG; 50 IU/kg body weight) on
   the 36th and 37th days after birth, and it was killed 24 h after the
   last treatment. The controls were injected with an equivalent volume of
   the corresponding vehicle. ACTH cells were immunohistochemically labeled
   and stereologically evaluated. Stereological analysis showed that the
   volume of ACTH cells and their volume density in peripubertal females
   treated with EDP, were decreased by 15.6\% and 53.8\% (p < 0.05),
   respectively, compared to the controls. In HCG-treated animals, the
   observed parameters were increased by 39.2\% and 15.4\% (p < 0.05),
   respectively, in comparison with the control females. These findings
   suggest that the application of EDP or HCG exerted opposite effects on
   the stereological features of pituitary ACTH cells.
PB  - Belgrade: Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - The effects of estradiol and human chorionic gonadotropin on ACHT cells in peripubertal female rats: A histological and stereological study
IS  - 1
VL  - 66
DO  - 10.2298/ABS1401143M
SP  - 143
EP  - 148
ER  - 

@article{
author = "Milošević, Verica and Todorović, Danijela and Šošić-Jurjević, Branka  and Jarić, Ivana and Živanović, Jasmina and Ušćebrka, Gordana and Ajdžanović, Vladimir",
year = "2014",
abstract = "The effects of estradiol (EDP) and human chorionic gonadotropin (HCG) on
   histological and stereological features of adrenocorticotropic (ACTH)
   cells in peripubertal female rats were examined. The first group of
   females received five injections of EDP (0.25 mg/kg b.w.), every second
   day from the 4th to 14th day after birth, and was killed at the
   peripubertal stage. The second group of females was given two injections
   of pregnyl-gonadotrophinum chorionicum (HCG; 50 IU/kg body weight) on
   the 36th and 37th days after birth, and it was killed 24 h after the
   last treatment. The controls were injected with an equivalent volume of
   the corresponding vehicle. ACTH cells were immunohistochemically labeled
   and stereologically evaluated. Stereological analysis showed that the
   volume of ACTH cells and their volume density in peripubertal females
   treated with EDP, were decreased by 15.6\% and 53.8\% (p < 0.05),
   respectively, compared to the controls. In HCG-treated animals, the
   observed parameters were increased by 39.2\% and 15.4\% (p < 0.05),
   respectively, in comparison with the control females. These findings
   suggest that the application of EDP or HCG exerted opposite effects on
   the stereological features of pituitary ACTH cells.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "The effects of estradiol and human chorionic gonadotropin on ACHT cells in peripubertal female rats: A histological and stereological study",
number = "1",
volume = "66",
doi = "10.2298/ABS1401143M",
pages = "143-148"
}

Milošević, V., Todorović, D., Šošić-Jurjević, B., Jarić, I., Živanović, J., Ušćebrka, G.,& Ajdžanović, V.. (2014). The effects of estradiol and human chorionic gonadotropin on ACHT cells in peripubertal female rats: A histological and stereological study. in Archives of Biological Sciences
Belgrade: Serbian Biological Society., 66(1), 143-148.
https://doi.org/10.2298/ABS1401143M

Milošević V, Todorović D, Šošić-Jurjević B, Jarić I, Živanović J, Ušćebrka G, Ajdžanović V. The effects of estradiol and human chorionic gonadotropin on ACHT cells in peripubertal female rats: A histological and stereological study. in Archives of Biological Sciences. 2014;66(1):143-148.
doi:10.2298/ABS1401143M .

Milošević, Verica, Todorović, Danijela, Šošić-Jurjević, Branka , Jarić, Ivana, Živanović, Jasmina, Ušćebrka, Gordana, Ajdžanović, Vladimir, "The effects of estradiol and human chorionic gonadotropin on ACHT cells in peripubertal female rats: A histological and stereological study" in Archives of Biological Sciences, 66, no. 1 (2014):143-148,
https://doi.org/10.2298/ABS1401143M . .

TY  - JOUR
AU  - Milošević, Verica
AU  - Ristić, Nataša
AU  - Todorović, Danijela
AU  - Veličković, Miroslava
AU  - Ušćebrka, Gordana
AU  - Knežević, Veroljub S.
AU  - Ajdžanović, Vladimir
PY  - 2012
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/497
AB  - Estradiol and human chorionic gonadotropin (hCG) are very important in controlling the secretory activity of hormone producing cells in the female rat pituitary glands. The aim of the present study was to examine the morphometric parameters of immunohistochemically la be led ACTH cells in juvenile (16th day) female rat pituitaries after treatment with five doses of estradiol dipropionate (EDP) and two doses of hCG during the neonatal period of life. The controls were treated on the same schedule with an equivalent volume of vehicle. All animals were sacrificed 24 h after the last treatment. ACTH-producing cells were studied using the peroxidase-antiperoxidase immunohistochemical procedure. The absolute and relative pituitary weights were increased (p<0.05) only in the EDP treated group by 120.0% and by 121.1% respectively, in comparison with the controls. In this group, the volume of ACTH cells, volume of their nuclei and volume density were significantly decreased (p<0.05) by 6.4%, 33.3% and 46.2% respectively, compared to the corresponding controls. After treatment with hCG, there were no significant (p>0.05) changes neither in the volume of ACTH cells nor in the volume of their nuclei, in comparison with the controls. On the basis of the results obtained in our study, it can be concluded that EDP, injected into female rats during the neonatal period of life, has an inhibitory effect on the immunohistomorphometric parameters of ACTH cells, but such an effect is not clearly expressed after treatment with hCG.
AB  - Estradiol i humani horionski gonadotropin (hCG) veoma su važni u kontroli sekretorne aktivnosti ćelija hipofize koje proizvode hormone. Cilj ove studije je bio ispitati morfometrijske parametre imunohistohemijski obeleženih ACTH ćelija hipofize ženki pacova, u juvenilnom periodu (16. dan), nakon tretmana sa pet doza estradiol-dipropionata (EDP) ili dve doze humanog horionskog gonadotropina (hCG). Kontrole su bile tretirane na istovetan način sa odgovarajućom količinom rastvarača. Sve životinje su bile žrtvovane 24 h nakon poslednjeg tretmana. ACTH ćelije su bile imunohistohemijski obeležene metodom peroksidaza-antiperoksidaza (PAP). Dobijeni rezultati ukazuju na značajno povećanje (p<0,05) apsolutne i relativne mase hipofize za 120% i 121,1% kod ženki tretiranih sa EDP u odnosu na kontrolu. U ovoj grupi ženki pacova volumen ACTH ćelija i njihovih jedara, kao i njihova volumenska gustina su bili značajno smanjeni (p<0,05) za 6,4%, 33,3% i 46,2% u poređenju sa kontrolom. Nakon tretmana hCG-om, volumen ACTH ćelija, kao i volumen njihovih jedara, nisu bili značajno (p>0,05) promenjeni u odnosu na kontrolu. Na osnovu dobijenih rezultata možemo zaključiti da EDP, injeciran ženkama pacova tokom neonatalnog perioda života, ima inhibitorni efekat na imuno histomorfometrijske parametre ACTH će lija, što nije tako jasno izraženo nakon tretmana hCG-om.
T2  - Journal of Medical Biochemistry
T1  - Imunohistomorfometrijske odlike ACTH ćelija u juvenilnih pacova nakon tretmana estradiolom ili humanim horionskim gonadotropinom
T1  - Immunohistomorphometric features of ACTH cells in juvenile rats after treatment with estradiol or human chorionic gonadotropin
IS  - 1
VL  - 31
SP  - 34
EP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_497
ER  - 

@article{
author = "Milošević, Verica and Ristić, Nataša and Todorović, Danijela and Veličković, Miroslava and Ušćebrka, Gordana and Knežević, Veroljub S. and Ajdžanović, Vladimir",
year = "2012, 2012",
abstract = "Estradiol and human chorionic gonadotropin (hCG) are very important in controlling the secretory activity of hormone producing cells in the female rat pituitary glands. The aim of the present study was to examine the morphometric parameters of immunohistochemically la be led ACTH cells in juvenile (16th day) female rat pituitaries after treatment with five doses of estradiol dipropionate (EDP) and two doses of hCG during the neonatal period of life. The controls were treated on the same schedule with an equivalent volume of vehicle. All animals were sacrificed 24 h after the last treatment. ACTH-producing cells were studied using the peroxidase-antiperoxidase immunohistochemical procedure. The absolute and relative pituitary weights were increased (p<0.05) only in the EDP treated group by 120.0% and by 121.1% respectively, in comparison with the controls. In this group, the volume of ACTH cells, volume of their nuclei and volume density were significantly decreased (p<0.05) by 6.4%, 33.3% and 46.2% respectively, compared to the corresponding controls. After treatment with hCG, there were no significant (p>0.05) changes neither in the volume of ACTH cells nor in the volume of their nuclei, in comparison with the controls. On the basis of the results obtained in our study, it can be concluded that EDP, injected into female rats during the neonatal period of life, has an inhibitory effect on the immunohistomorphometric parameters of ACTH cells, but such an effect is not clearly expressed after treatment with hCG., Estradiol i humani horionski gonadotropin (hCG) veoma su važni u kontroli sekretorne aktivnosti ćelija hipofize koje proizvode hormone. Cilj ove studije je bio ispitati morfometrijske parametre imunohistohemijski obeleženih ACTH ćelija hipofize ženki pacova, u juvenilnom periodu (16. dan), nakon tretmana sa pet doza estradiol-dipropionata (EDP) ili dve doze humanog horionskog gonadotropina (hCG). Kontrole su bile tretirane na istovetan način sa odgovarajućom količinom rastvarača. Sve životinje su bile žrtvovane 24 h nakon poslednjeg tretmana. ACTH ćelije su bile imunohistohemijski obeležene metodom peroksidaza-antiperoksidaza (PAP). Dobijeni rezultati ukazuju na značajno povećanje (p<0,05) apsolutne i relativne mase hipofize za 120% i 121,1% kod ženki tretiranih sa EDP u odnosu na kontrolu. U ovoj grupi ženki pacova volumen ACTH ćelija i njihovih jedara, kao i njihova volumenska gustina su bili značajno smanjeni (p<0,05) za 6,4%, 33,3% i 46,2% u poređenju sa kontrolom. Nakon tretmana hCG-om, volumen ACTH ćelija, kao i volumen njihovih jedara, nisu bili značajno (p>0,05) promenjeni u odnosu na kontrolu. Na osnovu dobijenih rezultata možemo zaključiti da EDP, injeciran ženkama pacova tokom neonatalnog perioda života, ima inhibitorni efekat na imuno histomorfometrijske parametre ACTH će lija, što nije tako jasno izraženo nakon tretmana hCG-om.",
journal = "Journal of Medical Biochemistry",
title = "Imunohistomorfometrijske odlike ACTH ćelija u juvenilnih pacova nakon tretmana estradiolom ili humanim horionskim gonadotropinom, Immunohistomorphometric features of ACTH cells in juvenile rats after treatment with estradiol or human chorionic gonadotropin",
number = "1",
volume = "31",
pages = "34-39",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_497"
}

Milošević, V., Ristić, N., Todorović, D., Veličković, M., Ušćebrka, G., Knežević, V. S.,& Ajdžanović, V.. (2012). Imunohistomorfometrijske odlike ACTH ćelija u juvenilnih pacova nakon tretmana estradiolom ili humanim horionskim gonadotropinom. in Journal of Medical Biochemistry, 31(1), 34-39.
https://hdl.handle.net/21.15107/rcub_ibiss_497

Milošević V, Ristić N, Todorović D, Veličković M, Ušćebrka G, Knežević VS, Ajdžanović V. Imunohistomorfometrijske odlike ACTH ćelija u juvenilnih pacova nakon tretmana estradiolom ili humanim horionskim gonadotropinom. in Journal of Medical Biochemistry. 2012;31(1):34-39.
https://hdl.handle.net/21.15107/rcub_ibiss_497 .

Milošević, Verica, Ristić, Nataša, Todorović, Danijela, Veličković, Miroslava, Ušćebrka, Gordana, Knežević, Veroljub S., Ajdžanović, Vladimir, "Imunohistomorfometrijske odlike ACTH ćelija u juvenilnih pacova nakon tretmana estradiolom ili humanim horionskim gonadotropinom" in Journal of Medical Biochemistry, 31, no. 1 (2012):34-39,
https://hdl.handle.net/21.15107/rcub_ibiss_497 .