TY  - CONF
AU  - Smailagić, Dijana
AU  - Dragišić Maksimović, Jelena
AU  - Marin, Marija
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Stupar, Sofija
AU  - Stanišić, Mariana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6710
AB  - Phloretin is a phenolic compound from the apple that belongs to the group of dihydrochalcones. The genus Malus is a uniquely rich source of dihydrochalcones, accounting for about 97% of the total phenolic compounds in apple leaves and 10-20% of the dry weight of the leaf tissue. Due to its typical dihydrochalcone structure, phloretin is a highly reactive molecule that interacts efficiently with many biological macromolecules. Its reactivity leads to remarkable antioxidant and medicinal properties that have been extensively studied in mammals and humans. However, knowledge about the effects of phloretin on plant organisms is rather scarce. The phytotoxicity of phloretin in plants was first documented by our research group when phloretin was recognised as a potent allelochemical of the apple tree with deleterious effects in plant species outside the genus Malus. Research on the model plant Arabidopsis [Arabidopsis thaliana (L.) Heynh] named the disruption of auxin homeostasis in the roots as the main mechanism of the phytotoxic effect of phloretin. The present study complements previous research with new insights into the ultrastructural changes in leaf mesophyll cells that underlie the growth arrest and decay of the aerial parts of Arabidopsis seedlings exposed to phloretin. The effects of phloretin are closely linked to the ultrastructural damage in the leaf mesophyll cells and range from mild effects with short-term treatment to severe effects with long-term treatment. Transmission electron microscopy revealed changes in the morphology of the cell wall, changes in thylakoid organisation, swelling and displacement of chloroplasts after short-term treatment. A significant decrease in the number of starch granules and chlorophyll content in the leaves reflected impaired chloroplast function leading to depletion of starch reserves, starvation of cells and recruitment of micro- and macroautophagic processes for self-maintenance leading to programmed cell death after long-term treatment with phloretin. These results confirm that the sites of action of phloretin in plant and animal cells do not coincide, confirming the safety of its potential application as a new bioherbicidal agent for humans and animals.
PB  - Turkey. Gebze,Turkey: Technical University
C3  - Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey
T1  - How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?
SP  - ID-739
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6710
ER  - 

@conference{
author = "Smailagić, Dijana and Dragišić Maksimović, Jelena and Marin, Marija and Banjac, Nevena and Ninković, Slavica and Stupar, Sofija and Stanišić, Mariana",
year = "2024",
abstract = "Phloretin is a phenolic compound from the apple that belongs to the group of dihydrochalcones. The genus Malus is a uniquely rich source of dihydrochalcones, accounting for about 97% of the total phenolic compounds in apple leaves and 10-20% of the dry weight of the leaf tissue. Due to its typical dihydrochalcone structure, phloretin is a highly reactive molecule that interacts efficiently with many biological macromolecules. Its reactivity leads to remarkable antioxidant and medicinal properties that have been extensively studied in mammals and humans. However, knowledge about the effects of phloretin on plant organisms is rather scarce. The phytotoxicity of phloretin in plants was first documented by our research group when phloretin was recognised as a potent allelochemical of the apple tree with deleterious effects in plant species outside the genus Malus. Research on the model plant Arabidopsis [Arabidopsis thaliana (L.) Heynh] named the disruption of auxin homeostasis in the roots as the main mechanism of the phytotoxic effect of phloretin. The present study complements previous research with new insights into the ultrastructural changes in leaf mesophyll cells that underlie the growth arrest and decay of the aerial parts of Arabidopsis seedlings exposed to phloretin. The effects of phloretin are closely linked to the ultrastructural damage in the leaf mesophyll cells and range from mild effects with short-term treatment to severe effects with long-term treatment. Transmission electron microscopy revealed changes in the morphology of the cell wall, changes in thylakoid organisation, swelling and displacement of chloroplasts after short-term treatment. A significant decrease in the number of starch granules and chlorophyll content in the leaves reflected impaired chloroplast function leading to depletion of starch reserves, starvation of cells and recruitment of micro- and macroautophagic processes for self-maintenance leading to programmed cell death after long-term treatment with phloretin. These results confirm that the sites of action of phloretin in plant and animal cells do not coincide, confirming the safety of its potential application as a new bioherbicidal agent for humans and animals.",
publisher = "Turkey. Gebze,Turkey: Technical University",
journal = "Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey",
title = "How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?",
pages = "ID-739",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6710"
}

Smailagić, D., Dragišić Maksimović, J., Marin, M., Banjac, N., Ninković, S., Stupar, S.,& Stanišić, M.. (2024). How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?. in Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey
Turkey. Gebze,Turkey: Technical University., ID-739.
https://hdl.handle.net/21.15107/rcub_ibiss_6710

Smailagić D, Dragišić Maksimović J, Marin M, Banjac N, Ninković S, Stupar S, Stanišić M. How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?. in Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey. 2024;:ID-739.
https://hdl.handle.net/21.15107/rcub_ibiss_6710 .

Smailagić, Dijana, Dragišić Maksimović, Jelena, Marin, Marija, Banjac, Nevena, Ninković, Slavica, Stupar, Sofija, Stanišić, Mariana, "How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?" in Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey (2024):ID-739,
https://hdl.handle.net/21.15107/rcub_ibiss_6710 .

TY  - CONF
AU  - Ninković, Slavica
AU  - Banjac, Nevena
AU  - Stanišić, Mariana
AU  - Smailagić, Dijana
AU  - Ćosić, Tatjana
AU  - Živanović, Branka
AU  - Dobrev, Petre I.
AU  - Motyka, Vaclav
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6112
AB  - Root-exuded compounds play a key role in the regulation of plant allelopathic interactions. In contrast to
numerous studies showing that many secondary metabolites have an allelopathic function, evidence of
exudation of phytohormones into the rhizosphere and their involvement in allelopathic interactions is scarce.
We postulated that phytohormones secreted by roots into the environment may act as allelochemicals that
affect the growth performance of neighboring plants. Hairy root culture media of two distinct species, the fruit
tree species Malus domestica cv. Golden delicious[1] and the herbaceous weed species Chenopodium murale
[2,3] were collected and analyzed by ultra-high-performance liquid chromatography coupled with tandem mass
spectrometry (UHPLC-MS/MS). We found that most of plant growth substances exuded by the hairy roots of
C. murale and M. domestica were assorted from fraction A (96.8% and even 98.9%, respectively) including
OxIAA, PAA, SA, BzA, and ABA, with SA and BzA being the most abundant, whereas those from fraction B,
including cytokinins and the ethylene precursor ACC, accounted for only 1% of the regulatory substances
detected in both species. Exogenous application of SA at concentrations released by the hairy roots of the
respective plant species and accumulated in the culture media for four weeks significantly affected the hairy
root growth of the other species and also the shoot and root growth of the Arabidopsis seedlings. The
disruptive effect of SA in the exuded concentration on the membrane potential of Arabidopsis root cells was
determined. The data obtained would be useful for designing further studies aimed at elucidating the
contribution and role of exuded phytohormones to overall allelopathic potential of these two plant species.
OxIAA = 2-oxindole-3-acetic acid, PAA = phenylacetic acid, SA = salicylic acid, BzA = benzoic acid, ABA =
abscisic acid, ACC = 1-aminocyclopropane-1-carboxylic acid
PB  - Daejeon: International Association For Plant Biotechnology
C3  - Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea
T1  - Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling
SP  - 484
EP  - 484
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6112
ER  - 

@conference{
author = "Ninković, Slavica and Banjac, Nevena and Stanišić, Mariana and Smailagić, Dijana and Ćosić, Tatjana and Živanović, Branka and Dobrev, Petre I. and Motyka, Vaclav",
year = "2023",
abstract = "Root-exuded compounds play a key role in the regulation of plant allelopathic interactions. In contrast to
numerous studies showing that many secondary metabolites have an allelopathic function, evidence of
exudation of phytohormones into the rhizosphere and their involvement in allelopathic interactions is scarce.
We postulated that phytohormones secreted by roots into the environment may act as allelochemicals that
affect the growth performance of neighboring plants. Hairy root culture media of two distinct species, the fruit
tree species Malus domestica cv. Golden delicious[1] and the herbaceous weed species Chenopodium murale
[2,3] were collected and analyzed by ultra-high-performance liquid chromatography coupled with tandem mass
spectrometry (UHPLC-MS/MS). We found that most of plant growth substances exuded by the hairy roots of
C. murale and M. domestica were assorted from fraction A (96.8% and even 98.9%, respectively) including
OxIAA, PAA, SA, BzA, and ABA, with SA and BzA being the most abundant, whereas those from fraction B,
including cytokinins and the ethylene precursor ACC, accounted for only 1% of the regulatory substances
detected in both species. Exogenous application of SA at concentrations released by the hairy roots of the
respective plant species and accumulated in the culture media for four weeks significantly affected the hairy
root growth of the other species and also the shoot and root growth of the Arabidopsis seedlings. The
disruptive effect of SA in the exuded concentration on the membrane potential of Arabidopsis root cells was
determined. The data obtained would be useful for designing further studies aimed at elucidating the
contribution and role of exuded phytohormones to overall allelopathic potential of these two plant species.
OxIAA = 2-oxindole-3-acetic acid, PAA = phenylacetic acid, SA = salicylic acid, BzA = benzoic acid, ABA =
abscisic acid, ACC = 1-aminocyclopropane-1-carboxylic acid",
publisher = "Daejeon: International Association For Plant Biotechnology",
journal = "Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea",
title = "Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling",
pages = "484-484",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6112"
}

Ninković, S., Banjac, N., Stanišić, M., Smailagić, D., Ćosić, T., Živanović, B., Dobrev, P. I.,& Motyka, V.. (2023). Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling. in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea
Daejeon: International Association For Plant Biotechnology., 484-484.
https://hdl.handle.net/21.15107/rcub_ibiss_6112

Ninković S, Banjac N, Stanišić M, Smailagić D, Ćosić T, Živanović B, Dobrev PI, Motyka V. Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling. in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea. 2023;:484-484.
https://hdl.handle.net/21.15107/rcub_ibiss_6112 .

Ninković, Slavica, Banjac, Nevena, Stanišić, Mariana, Smailagić, Dijana, Ćosić, Tatjana, Živanović, Branka, Dobrev, Petre I., Motyka, Vaclav, "Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling" in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea (2023):484-484,
https://hdl.handle.net/21.15107/rcub_ibiss_6112 .

TY  - CONF
AU  - Smailagić, Dijana
AU  - Dragišić Maksimović, Jelena
AU  - Stupar, Sofija
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Stanišić, Mariana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5067
AB  - Apple species are exclusively rich source of dihydrochalcones phloretin and phloridzin, phenolic compounds with elusive role in planta but suggested auto-allelochemical features related to 
‘apple replant disease’ (ARD). Our recent study emphasized phytotoxic action of phloretin towards 
other plant species such as model plant Arabidopsis as a consequence of disturbed auxin homeostasis in roots and altered chloroplast structure and function. Since elevated production and 
accumulation of reactive oxygen species (ROS) in plant cells is the one of the most common effect 
of allelochemicals, this study aimed to analyze the antioxidant status of Arabidopsis seedlings 
treated with phloretin. Total phenolic content (TPC) and total antioxidant capacity (TAC), as well 
as antioxidant enzymes activities were determined spectrophotometrically in treated and control 
seedlings at 5th and 10th day after germination (DAG). TAC increased c.a. 280% and 200% relative to 
control at 5th and 10th DAG respectively, presumably due to highly elevated TPC in treated seedlings (c.a. 150%). At the same time, the activity of antioxidant enzymes significantly decreased. The 
activity of catalase (CAT) was reduced by c.a. 40% and guaiacol peroxidase (POD) activities by 93% 
and 47% at 5th and 10th DAG, respectively. The activity of superoxide dismutase (SOD) was reduced 
by 55% and 18% at 5th and 10th DAG, respectively. The burst of antioxidant capacity of phloretin 
treated Arabidopsis seedlings resulted in significantly decreased antioxidant enzymes activities 
presumably due to strong antioxidant properties of absorbed phloretin. However, phloretin inhibition of antioxidant enzymatic activity due to its direct interaction with enzymes is object of 
further investigation.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Phloretin, dihydrochalcone from apple tree, bursts antioxidant capacity of tested Arabidopsis seedlings
SP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5067
ER  - 

@conference{
author = "Smailagić, Dijana and Dragišić Maksimović, Jelena and Stupar, Sofija and Banjac, Nevena and Ninković, Slavica and Stanišić, Mariana",
year = "2022",
abstract = "Apple species are exclusively rich source of dihydrochalcones phloretin and phloridzin, phenolic compounds with elusive role in planta but suggested auto-allelochemical features related to 
‘apple replant disease’ (ARD). Our recent study emphasized phytotoxic action of phloretin towards 
other plant species such as model plant Arabidopsis as a consequence of disturbed auxin homeostasis in roots and altered chloroplast structure and function. Since elevated production and 
accumulation of reactive oxygen species (ROS) in plant cells is the one of the most common effect 
of allelochemicals, this study aimed to analyze the antioxidant status of Arabidopsis seedlings 
treated with phloretin. Total phenolic content (TPC) and total antioxidant capacity (TAC), as well 
as antioxidant enzymes activities were determined spectrophotometrically in treated and control 
seedlings at 5th and 10th day after germination (DAG). TAC increased c.a. 280% and 200% relative to 
control at 5th and 10th DAG respectively, presumably due to highly elevated TPC in treated seedlings (c.a. 150%). At the same time, the activity of antioxidant enzymes significantly decreased. The 
activity of catalase (CAT) was reduced by c.a. 40% and guaiacol peroxidase (POD) activities by 93% 
and 47% at 5th and 10th DAG, respectively. The activity of superoxide dismutase (SOD) was reduced 
by 55% and 18% at 5th and 10th DAG, respectively. The burst of antioxidant capacity of phloretin 
treated Arabidopsis seedlings resulted in significantly decreased antioxidant enzymes activities 
presumably due to strong antioxidant properties of absorbed phloretin. However, phloretin inhibition of antioxidant enzymatic activity due to its direct interaction with enzymes is object of 
further investigation.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Phloretin, dihydrochalcone from apple tree, bursts antioxidant capacity of tested Arabidopsis seedlings",
pages = "73",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5067"
}

Smailagić, D., Dragišić Maksimović, J., Stupar, S., Banjac, N., Ninković, S.,& Stanišić, M.. (2022). Phloretin, dihydrochalcone from apple tree, bursts antioxidant capacity of tested Arabidopsis seedlings. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 73.
https://hdl.handle.net/21.15107/rcub_ibiss_5067

Smailagić D, Dragišić Maksimović J, Stupar S, Banjac N, Ninković S, Stanišić M. Phloretin, dihydrochalcone from apple tree, bursts antioxidant capacity of tested Arabidopsis seedlings. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:73.
https://hdl.handle.net/21.15107/rcub_ibiss_5067 .

Smailagić, Dijana, Dragišić Maksimović, Jelena, Stupar, Sofija, Banjac, Nevena, Ninković, Slavica, Stanišić, Mariana, "Phloretin, dihydrochalcone from apple tree, bursts antioxidant capacity of tested Arabidopsis seedlings" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):73,
https://hdl.handle.net/21.15107/rcub_ibiss_5067 .

TY  - CONF
AU  - Bondokić, Jasna
AU  - Trajković, Milena
AU  - Dragišić Maksimović, Jelena
AU  - Smailagić, Dijana
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Stanišić, Mariana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5064
AB  - Phloretin is phenolic compound specifically produced in apple tree species and secreted into the soil as a response to biotic and abiotic factors. Since our previous results indicated roots as the primary site of phloretin phytotoxic action, this study focused on assessment of phloretin effects on the root growth of weed (Chenopodium murale and Lotus corniculatus),  crop (Solanum tuberosum) and woody species (Castanea sativa and Malus × domestica Borkh.). Significant decrease in root biomass production in liquid growth medium enriched with 500 µM phloretin was observed both after 2 and 4 weeks of treatment in all tested species. The percentages of roots growth inhibition were as follows: Chenopodium murale (95% and 89,1%), Lotus corniculatus (69,4% and 81,8%), Solanum tuberosum (88,4% and 84,7%), Castanea sativa (85,6% and 100%) and Malus × domestica Borkh. (65,3% and 87,4%, respectively). Total antioxidant capacity (TAC), total phenolic content (TPC), as well as antioxidant enzymes activities were determined spectrophotometrically in treated and control Chenopodium murale roots after 4 weeks of phloretin treatment. TAC increased 44,1% presumably due to elevated TPC (25,8%) in treated roots. However, there were no significant differences in catalase (CAT), guaiacol peroxidase (POD) and superoxide dismutase (SOD) activities in control and treated roots after 4 weeks of treatment. Increased antioxidant activity in treated roots is presumably the consequence of known phloretin antioxidant potential.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - The effect of phloretin on root growth and antioxidant activity in selected plant species in vitro
SP  - 81
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5064
ER  - 

@conference{
author = "Bondokić, Jasna and Trajković, Milena and Dragišić Maksimović, Jelena and Smailagić, Dijana and Banjac, Nevena and Ninković, Slavica and Stanišić, Mariana",
year = "2022",
abstract = "Phloretin is phenolic compound specifically produced in apple tree species and secreted into the soil as a response to biotic and abiotic factors. Since our previous results indicated roots as the primary site of phloretin phytotoxic action, this study focused on assessment of phloretin effects on the root growth of weed (Chenopodium murale and Lotus corniculatus),  crop (Solanum tuberosum) and woody species (Castanea sativa and Malus × domestica Borkh.). Significant decrease in root biomass production in liquid growth medium enriched with 500 µM phloretin was observed both after 2 and 4 weeks of treatment in all tested species. The percentages of roots growth inhibition were as follows: Chenopodium murale (95% and 89,1%), Lotus corniculatus (69,4% and 81,8%), Solanum tuberosum (88,4% and 84,7%), Castanea sativa (85,6% and 100%) and Malus × domestica Borkh. (65,3% and 87,4%, respectively). Total antioxidant capacity (TAC), total phenolic content (TPC), as well as antioxidant enzymes activities were determined spectrophotometrically in treated and control Chenopodium murale roots after 4 weeks of phloretin treatment. TAC increased 44,1% presumably due to elevated TPC (25,8%) in treated roots. However, there were no significant differences in catalase (CAT), guaiacol peroxidase (POD) and superoxide dismutase (SOD) activities in control and treated roots after 4 weeks of treatment. Increased antioxidant activity in treated roots is presumably the consequence of known phloretin antioxidant potential.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "The effect of phloretin on root growth and antioxidant activity in selected plant species in vitro",
pages = "81",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5064"
}

Bondokić, J., Trajković, M., Dragišić Maksimović, J., Smailagić, D., Banjac, N., Ninković, S.,& Stanišić, M.. (2022). The effect of phloretin on root growth and antioxidant activity in selected plant species in vitro. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 81.
https://hdl.handle.net/21.15107/rcub_ibiss_5064

Bondokić J, Trajković M, Dragišić Maksimović J, Smailagić D, Banjac N, Ninković S, Stanišić M. The effect of phloretin on root growth and antioxidant activity in selected plant species in vitro. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:81.
https://hdl.handle.net/21.15107/rcub_ibiss_5064 .

Bondokić, Jasna, Trajković, Milena, Dragišić Maksimović, Jelena, Smailagić, Dijana, Banjac, Nevena, Ninković, Slavica, Stanišić, Mariana, "The effect of phloretin on root growth and antioxidant activity in selected plant species in vitro" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):81,
https://hdl.handle.net/21.15107/rcub_ibiss_5064 .

TY  - CONF
AU  - Smailagić, Dijana
AU  - Marin, Marija
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Stanišić, Mariana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5066
AB  - Phloretin is a phenolic compound unique for apple (Malus × domestica Borkh.) species. Our 
research group recently reported phytotoxic potential of phloretin towards other plant species 
such as model plant Arabidopsis. Since sugars in in vitro growth media could modulate plant 
metabolism, we analyzed phloretin effects on Arabidopsis seedlings grown on the medium with out sucrose and myoinositol (WSM) but enriched with different concentrations of phloretin (0 
to 1500 µM). Phloretin treatment on WSM induced seedlings growth retardation at much lower 
concentrations compared to sucrose and myoinositol enriched medium. Inhibition of primary 
root and leaf growth, and lateral root emergence increased with both phloretin concentration 
and treatment duration (maximal 85.2%, 30.4% and 46.5% at 1500 µM phloretin at 15 days after 
germination - DAG, respectively). Prolonged cultivation (4 weeks) at phloretin concentrations over 
500 µM induced complete growth stagnation and necrosis of the leaves. Transmission electron 
microscopy reviled relocation of chloroplasts within mesophyll cells, shape change into spherical 
form, as well as ultrastructural changes in thylakoid organization in leaves of 10 DAG-old seedlings 
treated with 250 or 500 µM phloretin. Significantly decreased number of starch grains in chloro plasts and chlorophyll a and b content in leaves reflected impaired function of chloroplasts due 
to ultrastructural changes. Phloretin also induced cell wall undulation and folds that resulted in 
irregularly shaped mesophyll cells. The presented study revealed that beside roots that had been 
previously emphasized as primary targets of phloretin action, leaves were also significantly affect ed by phloretin treatment, especially in the aspect of photosynthetic apparatus
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Morpho-physiological and ultrastructural changes of Arabidopsis seedlings treated with phloretin
SP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5066
ER  - 

@conference{
author = "Smailagić, Dijana and Marin, Marija and Banjac, Nevena and Ninković, Slavica and Stanišić, Mariana",
year = "2022",
abstract = "Phloretin is a phenolic compound unique for apple (Malus × domestica Borkh.) species. Our 
research group recently reported phytotoxic potential of phloretin towards other plant species 
such as model plant Arabidopsis. Since sugars in in vitro growth media could modulate plant 
metabolism, we analyzed phloretin effects on Arabidopsis seedlings grown on the medium with out sucrose and myoinositol (WSM) but enriched with different concentrations of phloretin (0 
to 1500 µM). Phloretin treatment on WSM induced seedlings growth retardation at much lower 
concentrations compared to sucrose and myoinositol enriched medium. Inhibition of primary 
root and leaf growth, and lateral root emergence increased with both phloretin concentration 
and treatment duration (maximal 85.2%, 30.4% and 46.5% at 1500 µM phloretin at 15 days after 
germination - DAG, respectively). Prolonged cultivation (4 weeks) at phloretin concentrations over 
500 µM induced complete growth stagnation and necrosis of the leaves. Transmission electron 
microscopy reviled relocation of chloroplasts within mesophyll cells, shape change into spherical 
form, as well as ultrastructural changes in thylakoid organization in leaves of 10 DAG-old seedlings 
treated with 250 or 500 µM phloretin. Significantly decreased number of starch grains in chloro plasts and chlorophyll a and b content in leaves reflected impaired function of chloroplasts due 
to ultrastructural changes. Phloretin also induced cell wall undulation and folds that resulted in 
irregularly shaped mesophyll cells. The presented study revealed that beside roots that had been 
previously emphasized as primary targets of phloretin action, leaves were also significantly affect ed by phloretin treatment, especially in the aspect of photosynthetic apparatus",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Morpho-physiological and ultrastructural changes of Arabidopsis seedlings treated with phloretin",
pages = "72",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5066"
}

Smailagić, D., Marin, M., Banjac, N., Ninković, S.,& Stanišić, M.. (2022). Morpho-physiological and ultrastructural changes of Arabidopsis seedlings treated with phloretin. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 72.
https://hdl.handle.net/21.15107/rcub_ibiss_5066

Smailagić D, Marin M, Banjac N, Ninković S, Stanišić M. Morpho-physiological and ultrastructural changes of Arabidopsis seedlings treated with phloretin. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:72.
https://hdl.handle.net/21.15107/rcub_ibiss_5066 .

Smailagić, Dijana, Marin, Marija, Banjac, Nevena, Ninković, Slavica, Stanišić, Mariana, "Morpho-physiological and ultrastructural changes of Arabidopsis seedlings treated with phloretin" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):72,
https://hdl.handle.net/21.15107/rcub_ibiss_5066 .

TY  - CONF
AU  - Stanišić, Mariana
AU  - Smailagić, Dijana
AU  - Živanović, Branka
AU  - Maksimović, Vuk
AU  - Ninković, Slavica
AU  - Banjac, Nevena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5063
AB  - Phloretin, phenolic compound found exclusively in Apple trees, possesses bioherbicidal potential towards other plant species. Our recent studies emphasized phloretin phytotoxic action towards model plant Arabidopsis [Arabidopsis thaliana (L.) Heynh.] as a consequence of disturbed auxin homeostasis in roots and disrupted normal leaf cell function due to chloroplast ultrastructural changes and decreased chlorophyll a and b levels. Given the known phloretin reactivity that results from its flexible dihydrochalcones structure, this study aimed to elucidate phloretin direct effects on the root plasma membrane, as well as phloretin post-treatment metabolism in Arabidopsis plants. Electrophysiological measurements were performed to assess real-time response of root plasma membrane to exogenously applied phloretin. Exposing Arabidopsis roots to a 250 µM phloretin resulted in rapid and significant depolarization of the root membrane potential with amplitude of about 47mV that confirmed high sensitivity of Arabidopsis to phloretin treatment. Reverse-phase liquid chromatography with mass spectrometry (LC-MS) was used to evaluate post-treatment metabolism of phloretin in Arabidopsis seedlings grown on 500 µM phloretin enriched medium for 10 days. Analyses revealed that phloretin underwent intensive glycosylation within plant tissues. Dominant peaks from treated plants showed a palette of phloretin derivatives mainly mono- and di-glycosides. Their quantity was significantly higher than in control plants, suggesting high uptake and metabolic interconversion of phloretin as an attempt of phloretin detoxification and/or preparation for vacuolar sequestration.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - What happens with phloretin in plants? – Phloretin real-time effects and post-treatment metabolism in treated Arabidopsis seedlings
SP  - 44
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5063
ER  - 

@conference{
author = "Stanišić, Mariana and Smailagić, Dijana and Živanović, Branka and Maksimović, Vuk and Ninković, Slavica and Banjac, Nevena",
year = "2022",
abstract = "Phloretin, phenolic compound found exclusively in Apple trees, possesses bioherbicidal potential towards other plant species. Our recent studies emphasized phloretin phytotoxic action towards model plant Arabidopsis [Arabidopsis thaliana (L.) Heynh.] as a consequence of disturbed auxin homeostasis in roots and disrupted normal leaf cell function due to chloroplast ultrastructural changes and decreased chlorophyll a and b levels. Given the known phloretin reactivity that results from its flexible dihydrochalcones structure, this study aimed to elucidate phloretin direct effects on the root plasma membrane, as well as phloretin post-treatment metabolism in Arabidopsis plants. Electrophysiological measurements were performed to assess real-time response of root plasma membrane to exogenously applied phloretin. Exposing Arabidopsis roots to a 250 µM phloretin resulted in rapid and significant depolarization of the root membrane potential with amplitude of about 47mV that confirmed high sensitivity of Arabidopsis to phloretin treatment. Reverse-phase liquid chromatography with mass spectrometry (LC-MS) was used to evaluate post-treatment metabolism of phloretin in Arabidopsis seedlings grown on 500 µM phloretin enriched medium for 10 days. Analyses revealed that phloretin underwent intensive glycosylation within plant tissues. Dominant peaks from treated plants showed a palette of phloretin derivatives mainly mono- and di-glycosides. Their quantity was significantly higher than in control plants, suggesting high uptake and metabolic interconversion of phloretin as an attempt of phloretin detoxification and/or preparation for vacuolar sequestration.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "What happens with phloretin in plants? – Phloretin real-time effects and post-treatment metabolism in treated Arabidopsis seedlings",
pages = "44",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5063"
}

Stanišić, M., Smailagić, D., Živanović, B., Maksimović, V., Ninković, S.,& Banjac, N.. (2022). What happens with phloretin in plants? – Phloretin real-time effects and post-treatment metabolism in treated Arabidopsis seedlings. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 44.
https://hdl.handle.net/21.15107/rcub_ibiss_5063

Stanišić M, Smailagić D, Živanović B, Maksimović V, Ninković S, Banjac N. What happens with phloretin in plants? – Phloretin real-time effects and post-treatment metabolism in treated Arabidopsis seedlings. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:44.
https://hdl.handle.net/21.15107/rcub_ibiss_5063 .

Stanišić, Mariana, Smailagić, Dijana, Živanović, Branka, Maksimović, Vuk, Ninković, Slavica, Banjac, Nevena, "What happens with phloretin in plants? – Phloretin real-time effects and post-treatment metabolism in treated Arabidopsis seedlings" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):44,
https://hdl.handle.net/21.15107/rcub_ibiss_5063 .

TY  - JOUR
AU  - Smailagić, Dijana
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Savić, Jelena
AU  - Ćosić, Tatjana
AU  - Pěnčík, Aleš
AU  - Ćalić, Dušica
AU  - Bogdanović, Milica
AU  - Trajković, Milena
AU  - Stanišić, Mariana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5065
AB  - Apple species are the unique naturally rich source of dihydrochalcones, phenolic compounds with an elusive role in planta, but suggested auto-allelochemical features related to “apple replant disease” (ARD). Our aim was to elucidate the physiological basis of the phytotoxic action of dihydrochalcone phloretin in the model plant Arabidopsis and to promote phloretin as a new prospective eco-friendly phytotoxic compound. Phloretin treatment induced a significant dose-dependent growth retardation and severe morphological abnormalities and agravitropic behavior in Arabidopsis seedlings. Histological examination revealed a reduced starch content in the columella cells and a serious disturbance in root architecture, which resulted in the reduction in length of meristematic and elongation zones. Significantly disturbed auxin metabolome profile in roots with a particularly increased content of IAA accumulated in the lateral parts of the root apex, accompanied by changes in the expression of auxin biosynthetic and transport genes, especially PIN1, PIN3, PIN7, and ABCB1, indicates the role of auxin in physiological basis of phloretin-induced growth retardation. The results reveal a disturbance of auxin homeostasis as the main mechanism of phytotoxic action of phloretin. This mechanism makes phloretin a prospective candidate for an eco-friendly bioherbicide and paves the way for further research of phloretin role in ARD.
PB  - Lausanne: Frontiers Media SA
T2  - Frontiers in Plant Science
T1  - New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action
VL  - 13
DO  - 0.3389/fpls.2022.875528
SP  - 875528
ER  - 

@article{
author = "Smailagić, Dijana and Banjac, Nevena and Ninković, Slavica and Savić, Jelena and Ćosić, Tatjana and Pěnčík, Aleš and Ćalić, Dušica and Bogdanović, Milica and Trajković, Milena and Stanišić, Mariana",
year = "2022",
abstract = "Apple species are the unique naturally rich source of dihydrochalcones, phenolic compounds with an elusive role in planta, but suggested auto-allelochemical features related to “apple replant disease” (ARD). Our aim was to elucidate the physiological basis of the phytotoxic action of dihydrochalcone phloretin in the model plant Arabidopsis and to promote phloretin as a new prospective eco-friendly phytotoxic compound. Phloretin treatment induced a significant dose-dependent growth retardation and severe morphological abnormalities and agravitropic behavior in Arabidopsis seedlings. Histological examination revealed a reduced starch content in the columella cells and a serious disturbance in root architecture, which resulted in the reduction in length of meristematic and elongation zones. Significantly disturbed auxin metabolome profile in roots with a particularly increased content of IAA accumulated in the lateral parts of the root apex, accompanied by changes in the expression of auxin biosynthetic and transport genes, especially PIN1, PIN3, PIN7, and ABCB1, indicates the role of auxin in physiological basis of phloretin-induced growth retardation. The results reveal a disturbance of auxin homeostasis as the main mechanism of phytotoxic action of phloretin. This mechanism makes phloretin a prospective candidate for an eco-friendly bioherbicide and paves the way for further research of phloretin role in ARD.",
publisher = "Lausanne: Frontiers Media SA",
journal = "Frontiers in Plant Science",
title = "New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action",
volume = "13",
doi = "0.3389/fpls.2022.875528",
pages = "875528"
}

Smailagić, D., Banjac, N., Ninković, S., Savić, J., Ćosić, T., Pěnčík, A., Ćalić, D., Bogdanović, M., Trajković, M.,& Stanišić, M.. (2022). New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action. in Frontiers in Plant Science
Lausanne: Frontiers Media SA., 13, 875528.
https://doi.org/0.3389/fpls.2022.875528

Smailagić D, Banjac N, Ninković S, Savić J, Ćosić T, Pěnčík A, Ćalić D, Bogdanović M, Trajković M, Stanišić M. New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action. in Frontiers in Plant Science. 2022;13:875528.
doi:0.3389/fpls.2022.875528 .

Smailagić, Dijana, Banjac, Nevena, Ninković, Slavica, Savić, Jelena, Ćosić, Tatjana, Pěnčík, Aleš, Ćalić, Dušica, Bogdanović, Milica, Trajković, Milena, Stanišić, Mariana, "New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action" in Frontiers in Plant Science, 13 (2022):875528,
https://doi.org/0.3389/fpls.2022.875528 . .