TY  - JOUR
AU  - Bani, Luciano
AU  - Orioli, Valerio
AU  - Giacchini, Roberto
AU  - Parenti, Paolo
AU  - Dondina, Olivia
AU  - Prokić, Marko
AU  - Faggio, Caterina
AU  - Campli, Giulia
PY  - 2022
UR  - https://onlinelibrary.wiley.com/doi/10.1111/oik.09292
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5070
AB  - Animal populations are increasingly forced to live in small residual natural or semi-natural areas due to habitat loss and fragmentation. Here, the viability of populations is often compromised by intrinsic threat factors typical of small and isolated populations, such as inbreeding depression, genetic drift and environmental and demographic stochasticity. Under these circumstances, organisms may have low fitness due to inadequate physiological responses needed to face environmental challenges. However, few studies have investigated the relationship between habitat fragmentation and stress defences. In this study, we aimed to test whether an increase in the level of individual inbreeding produced an increase in the antioxidant system response. To this purpose, we genotyped 151 individuals of fire salamander Salamandra salamandra (Amphibia: Urodela) within five sampling populations, located in forest landscapes with different degree of fragmentation in northern Italy. For 113 individuals we also measured the glutathione-S-transferase (GST) and catalase (CAT) enzyme activity. Results showed a significant increase in individual GST activity for increasing levels of inbreeding, whereas no relationship was found for CAT. We also measured acetylcholinesterase to test the possible confounding effects of pesticides that might have occurred in fragmented landscapes with forests interspersed with agricultural areas. However, no difference in this enzyme activity was found among sampling populations. We argue that high levels of GST activity may be symptomatic of oxidative stress derived from inbreeding. An increased frequency of homozygous deleterious alleles due to inbreeding may cause homeostatic alterations and trigger the expression of GST for protection against hydrogen peroxide reactive oxygen species. We suggest using GST as a biomarker for environmental stressors with great caution and not to underestimate that the sources of stress deriving from habitat fragmentation could lead to an unbalance in the oxidative status, possibly increasing population susceptibility to infectious diseases and, potentially, spillover events and zoonoses.
PB  - Hoboken: Wiley
T2  - Oikos
T1  - Can antioxidant responses be induced by habitat fragmentation process?
IS  - 9
VL  - 2022
DO  - 10.1111/oik.09292
SP  - e09292
ER  - 

@article{
author = "Bani, Luciano and Orioli, Valerio and Giacchini, Roberto and Parenti, Paolo and Dondina, Olivia and Prokić, Marko and Faggio, Caterina and Campli, Giulia",
year = "2022",
abstract = "Animal populations are increasingly forced to live in small residual natural or semi-natural areas due to habitat loss and fragmentation. Here, the viability of populations is often compromised by intrinsic threat factors typical of small and isolated populations, such as inbreeding depression, genetic drift and environmental and demographic stochasticity. Under these circumstances, organisms may have low fitness due to inadequate physiological responses needed to face environmental challenges. However, few studies have investigated the relationship between habitat fragmentation and stress defences. In this study, we aimed to test whether an increase in the level of individual inbreeding produced an increase in the antioxidant system response. To this purpose, we genotyped 151 individuals of fire salamander Salamandra salamandra (Amphibia: Urodela) within five sampling populations, located in forest landscapes with different degree of fragmentation in northern Italy. For 113 individuals we also measured the glutathione-S-transferase (GST) and catalase (CAT) enzyme activity. Results showed a significant increase in individual GST activity for increasing levels of inbreeding, whereas no relationship was found for CAT. We also measured acetylcholinesterase to test the possible confounding effects of pesticides that might have occurred in fragmented landscapes with forests interspersed with agricultural areas. However, no difference in this enzyme activity was found among sampling populations. We argue that high levels of GST activity may be symptomatic of oxidative stress derived from inbreeding. An increased frequency of homozygous deleterious alleles due to inbreeding may cause homeostatic alterations and trigger the expression of GST for protection against hydrogen peroxide reactive oxygen species. We suggest using GST as a biomarker for environmental stressors with great caution and not to underestimate that the sources of stress deriving from habitat fragmentation could lead to an unbalance in the oxidative status, possibly increasing population susceptibility to infectious diseases and, potentially, spillover events and zoonoses.",
publisher = "Hoboken: Wiley",
journal = "Oikos",
title = "Can antioxidant responses be induced by habitat fragmentation process?",
number = "9",
volume = "2022",
doi = "10.1111/oik.09292",
pages = "e09292"
}

Bani, L., Orioli, V., Giacchini, R., Parenti, P., Dondina, O., Prokić, M., Faggio, C.,& Campli, G.. (2022). Can antioxidant responses be induced by habitat fragmentation process?. in Oikos
Hoboken: Wiley., 2022(9), e09292.
https://doi.org/10.1111/oik.09292

Bani L, Orioli V, Giacchini R, Parenti P, Dondina O, Prokić M, Faggio C, Campli G. Can antioxidant responses be induced by habitat fragmentation process?. in Oikos. 2022;2022(9):e09292.
doi:10.1111/oik.09292 .

Bani, Luciano, Orioli, Valerio, Giacchini, Roberto, Parenti, Paolo, Dondina, Olivia, Prokić, Marko, Faggio, Caterina, Campli, Giulia, "Can antioxidant responses be induced by habitat fragmentation process?" in Oikos, 2022, no. 9 (2022):e09292,
https://doi.org/10.1111/oik.09292 . .

TY  - JOUR
AU  - Rashidian, Ghasem
AU  - Shahin, Khalid
AU  - Elshopakey, Gehad E.
AU  - Mahboub, Heba H.
AU  - Fahim, Azin
AU  - Elabd, Hiam
AU  - Prokić, Marko
AU  - Faggio, Caterina
PY  - 2022
UR  - https://www.mdpi.com/2077-1312/10/3/325
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4893
AB  - Medicinal plants are increasingly used in aquaculture owing to their beneficial impacts on the health status of farmed fish. The current study was conducted to investigate the effect of nutmeg (Myristica fragrans) extract on growth, immunity, antioxidant parameters, and resistance of common carp (Cyprinus carpio) against Aeromonas hydrophila. In addition, in vitro antibacterial activity of the skin mucus of fish fed on nutmeg extract was evaluated against three major fish pathogenic bacteria through the standard disk diffusion method. Fish (17.27 ± 0.11 g) were divided into four groups and fed on experimental diets containing different levels of nutmeg extract, including zero (control), 0.5% (M1), 1% (M2), and 2% (M3) per kg diet. Results showed that nutmeg significantly enhanced growth parameters after a four-week feeding trial. Feed conversion ratio was remarkably reduced with the lowest value reported for the M3 group, whereas weight gain was notably increased in M2 and M3. No significant effect was found on the hematological profile, including mean corpuscular hemoglobin concentration, mean corpuscular hemoglobin, mean corpuscular volume, and hematocrit, while the highest levels of red blood cells and white blood cells were found in the M3 group. Stress biomarkers, including glucose and cortisol, were the lowest in the M3 group. Serum and skin mucus immunological and antioxidant parameters were significantly higher in M3, followed by M2, where the highest resistance was also observed. In addition, skin mucus samples effectively inhibited Streptococcus iniae, Yersinia ruckeri, and Aeromonas hydrophila. Overall, the present results suggest that dietary nutmeg (20 g/kg diet) could be used as a growth promotor and immunostimulant in common carp.
PB  - Basel: MDPI
T2  - Journal of Marine Science and Engineering
T1  - The Dietary Effects of Nutmeg (Myristica fragrans) Extract on Growth, Hematological Parameters, Immunity, Antioxidant Status, and Disease Resistance of Common Carp (Cyprinus carpio) against Aeromonas hydrophila
IS  - 3
VL  - 10
DO  - 10.3390/jmse10030325
SP  - 325
ER  - 

@article{
author = "Rashidian, Ghasem and Shahin, Khalid and Elshopakey, Gehad E. and Mahboub, Heba H. and Fahim, Azin and Elabd, Hiam and Prokić, Marko and Faggio, Caterina",
year = "2022",
abstract = "Medicinal plants are increasingly used in aquaculture owing to their beneficial impacts on the health status of farmed fish. The current study was conducted to investigate the effect of nutmeg (Myristica fragrans) extract on growth, immunity, antioxidant parameters, and resistance of common carp (Cyprinus carpio) against Aeromonas hydrophila. In addition, in vitro antibacterial activity of the skin mucus of fish fed on nutmeg extract was evaluated against three major fish pathogenic bacteria through the standard disk diffusion method. Fish (17.27 ± 0.11 g) were divided into four groups and fed on experimental diets containing different levels of nutmeg extract, including zero (control), 0.5% (M1), 1% (M2), and 2% (M3) per kg diet. Results showed that nutmeg significantly enhanced growth parameters after a four-week feeding trial. Feed conversion ratio was remarkably reduced with the lowest value reported for the M3 group, whereas weight gain was notably increased in M2 and M3. No significant effect was found on the hematological profile, including mean corpuscular hemoglobin concentration, mean corpuscular hemoglobin, mean corpuscular volume, and hematocrit, while the highest levels of red blood cells and white blood cells were found in the M3 group. Stress biomarkers, including glucose and cortisol, were the lowest in the M3 group. Serum and skin mucus immunological and antioxidant parameters were significantly higher in M3, followed by M2, where the highest resistance was also observed. In addition, skin mucus samples effectively inhibited Streptococcus iniae, Yersinia ruckeri, and Aeromonas hydrophila. Overall, the present results suggest that dietary nutmeg (20 g/kg diet) could be used as a growth promotor and immunostimulant in common carp.",
publisher = "Basel: MDPI",
journal = "Journal of Marine Science and Engineering",
title = "The Dietary Effects of Nutmeg (Myristica fragrans) Extract on Growth, Hematological Parameters, Immunity, Antioxidant Status, and Disease Resistance of Common Carp (Cyprinus carpio) against Aeromonas hydrophila",
number = "3",
volume = "10",
doi = "10.3390/jmse10030325",
pages = "325"
}

Rashidian, G., Shahin, K., Elshopakey, G. E., Mahboub, H. H., Fahim, A., Elabd, H., Prokić, M.,& Faggio, C.. (2022). The Dietary Effects of Nutmeg (Myristica fragrans) Extract on Growth, Hematological Parameters, Immunity, Antioxidant Status, and Disease Resistance of Common Carp (Cyprinus carpio) against Aeromonas hydrophila. in Journal of Marine Science and Engineering
Basel: MDPI., 10(3), 325.
https://doi.org/10.3390/jmse10030325

Rashidian G, Shahin K, Elshopakey GE, Mahboub HH, Fahim A, Elabd H, Prokić M, Faggio C. The Dietary Effects of Nutmeg (Myristica fragrans) Extract on Growth, Hematological Parameters, Immunity, Antioxidant Status, and Disease Resistance of Common Carp (Cyprinus carpio) against Aeromonas hydrophila. in Journal of Marine Science and Engineering. 2022;10(3):325.
doi:10.3390/jmse10030325 .

Rashidian, Ghasem, Shahin, Khalid, Elshopakey, Gehad E., Mahboub, Heba H., Fahim, Azin, Elabd, Hiam, Prokić, Marko, Faggio, Caterina, "The Dietary Effects of Nutmeg (Myristica fragrans) Extract on Growth, Hematological Parameters, Immunity, Antioxidant Status, and Disease Resistance of Common Carp (Cyprinus carpio) against Aeromonas hydrophila" in Journal of Marine Science and Engineering, 10, no. 3 (2022):325,
https://doi.org/10.3390/jmse10030325 . .

TY  - JOUR
AU  - Vali, Sara
AU  - Majidiyan, Nava
AU  - Yalsuyi, Ahmad Mohamadi
AU  - Vajargah, Mohammad Forouhar
AU  - Prokić, Marko
AU  - Faggio, Caterina
PY  - 2022
UR  - https://www.mdpi.com/2073-4441/14/2/144
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4770
AB  - Nanoparticles (NPs) can display toxicological effects on aquatic organisms. This study investigates ecotoxicological effects of Ag-NPs on reproductive and blood parameters of adult common molly (Poecilia sphenops) and their larvae. During the LC50 96 h test, female fish were exposed to concentrations of 0, 5, 15, 25, 35, 45 and 60 mg L−1 of Ag-NPs, while larvae were exposed to 0, 3, 5, 10 and 15 mg L−1. Finally, we aim to evaluate the effects of 0, 5, 10 and 15 mg L−1 of Ag-NPs on parturition time, reproductive success and hematological parameters of the mature fish exposed to sub-lethal concentration during a 62-day period. We also evaluated the survival rate of larvae. The results show a positive correlation between mortality rate and Ag-NP concentration. Values for LC50 96 h in adult fish and larvae were 26.85 mg L−1 and 6.22 mg L−1, respectively. A lack of parturition and reproductive success were seen in fish that underwent chronic exposure to Ag-NPs (15 mg L−1). The results show that RBC, WBC and hematocrit were significantly decreased in fish exposed to Ag-NPs. In addition, the serum concentrations of total protein, albumin, cholesterol and triglycerides were significantly increased in fish submitted to Ag-NPs (concentrations of 5–15). In conclusion, submitting a fish to higher concentration than 10 mg L−1 has adverse effects on reproductive system and blood parameters.
PB  - Basel: MDPI
T2  - Water
T1  - Ecotoxicological Effects of Silver Nanoparticles (Ag-NPs) on Parturition Time, Survival Rate, Reproductive Success and Blood Parameters of Adult Common Molly (Poecilia sphenops) and Their Larvae
IS  - 2
VL  - 14
DO  - 10.3390/w14020144
SP  - 144
ER  - 

@article{
author = "Vali, Sara and Majidiyan, Nava and Yalsuyi, Ahmad Mohamadi and Vajargah, Mohammad Forouhar and Prokić, Marko and Faggio, Caterina",
year = "2022",
abstract = "Nanoparticles (NPs) can display toxicological effects on aquatic organisms. This study investigates ecotoxicological effects of Ag-NPs on reproductive and blood parameters of adult common molly (Poecilia sphenops) and their larvae. During the LC50 96 h test, female fish were exposed to concentrations of 0, 5, 15, 25, 35, 45 and 60 mg L−1 of Ag-NPs, while larvae were exposed to 0, 3, 5, 10 and 15 mg L−1. Finally, we aim to evaluate the effects of 0, 5, 10 and 15 mg L−1 of Ag-NPs on parturition time, reproductive success and hematological parameters of the mature fish exposed to sub-lethal concentration during a 62-day period. We also evaluated the survival rate of larvae. The results show a positive correlation between mortality rate and Ag-NP concentration. Values for LC50 96 h in adult fish and larvae were 26.85 mg L−1 and 6.22 mg L−1, respectively. A lack of parturition and reproductive success were seen in fish that underwent chronic exposure to Ag-NPs (15 mg L−1). The results show that RBC, WBC and hematocrit were significantly decreased in fish exposed to Ag-NPs. In addition, the serum concentrations of total protein, albumin, cholesterol and triglycerides were significantly increased in fish submitted to Ag-NPs (concentrations of 5–15). In conclusion, submitting a fish to higher concentration than 10 mg L−1 has adverse effects on reproductive system and blood parameters.",
publisher = "Basel: MDPI",
journal = "Water",
title = "Ecotoxicological Effects of Silver Nanoparticles (Ag-NPs) on Parturition Time, Survival Rate, Reproductive Success and Blood Parameters of Adult Common Molly (Poecilia sphenops) and Their Larvae",
number = "2",
volume = "14",
doi = "10.3390/w14020144",
pages = "144"
}

Vali, S., Majidiyan, N., Yalsuyi, A. M., Vajargah, M. F., Prokić, M.,& Faggio, C.. (2022). Ecotoxicological Effects of Silver Nanoparticles (Ag-NPs) on Parturition Time, Survival Rate, Reproductive Success and Blood Parameters of Adult Common Molly (Poecilia sphenops) and Their Larvae. in Water
Basel: MDPI., 14(2), 144.
https://doi.org/10.3390/w14020144

Vali S, Majidiyan N, Yalsuyi AM, Vajargah MF, Prokić M, Faggio C. Ecotoxicological Effects of Silver Nanoparticles (Ag-NPs) on Parturition Time, Survival Rate, Reproductive Success and Blood Parameters of Adult Common Molly (Poecilia sphenops) and Their Larvae. in Water. 2022;14(2):144.
doi:10.3390/w14020144 .

Vali, Sara, Majidiyan, Nava, Yalsuyi, Ahmad Mohamadi, Vajargah, Mohammad Forouhar, Prokić, Marko, Faggio, Caterina, "Ecotoxicological Effects of Silver Nanoparticles (Ag-NPs) on Parturition Time, Survival Rate, Reproductive Success and Blood Parameters of Adult Common Molly (Poecilia sphenops) and Their Larvae" in Water, 14, no. 2 (2022):144,
https://doi.org/10.3390/w14020144 . .

TY  - JOUR
AU  - Rashidian, Ghasem
AU  - Mahboub, Heba H.
AU  - Fahim, Azin
AU  - Hefny, Ahmed A.
AU  - Prokić, Marko
AU  - Rainis, Simona
AU  - Boldaji, Javad Tahmasebi
AU  - Faggio, Caterina
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S1050464821004289
UR  - http://www.ncbi.nlm.nih.gov/pubmed/34910977
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4747
AB  - In large-scale aquaculture, the fast growth rate of fish is positively influenced by feed additives such as medicinal plants. This is however; infectious disease may reduce fish growth and cause devastating economic loss. The present study investigated in vitro antibacterial efficacy of Mooseer (Allium hirtifolium) extract against Streptococcus iniae and its in vivo effects on growth, biochemical parameters, innate immunity of rainbow trout (Oncorhynchus mykiss). Therefore, six experimental diets were designed to include different levels of Mooseer from zero (as control), 5, 10, 15, 20, and 25 g per kg diet respectively referred to as M1 to M5. Results from the antibacterial evaluation showed that Mooseer extract inhibits S. iniae growth with MIC and MBC values of 128 and 256 μg ml-1. Appreciable results were obtained in the groups supplemented with Mooseer. Mooseer enhanced growth performance, and modulated serum biochemical and immunological parameters (total protein, albumin, triglyceride, glucose, cortisol, cholesterol, lysozyme, Ig, ACH50, ALP, and protease activity), and liver enzymes (ALT, AST and ALP). The greatest effects were found for higher doses of Mooseer supplementation (M4 and M5). Meanwhile, results from the survival rate of fish challenged with S. iniae showed higher survival in M2 and M4 treatments. The present findings suggest the beneficial use of Mooseer in rainbow trout diet, with 20 g kg-1 inclusion as the recommended dose.
PB  - London: Academic Press
T2  - Fish & Shellfish Immunology
T1  - Mooseer (Allium hirtifolium) boosts growth, general health status, and resistance of rainbow trout (Oncorhynchus mykiss) against Streptococcus iniae infection.
VL  - 120
DO  - 10.1016/j.fsi.2021.12.012
SP  - 360
EP  - 368
ER  - 

@article{
author = "Rashidian, Ghasem and Mahboub, Heba H. and Fahim, Azin and Hefny, Ahmed A. and Prokić, Marko and Rainis, Simona and Boldaji, Javad Tahmasebi and Faggio, Caterina",
year = "2022",
abstract = "In large-scale aquaculture, the fast growth rate of fish is positively influenced by feed additives such as medicinal plants. This is however; infectious disease may reduce fish growth and cause devastating economic loss. The present study investigated in vitro antibacterial efficacy of Mooseer (Allium hirtifolium) extract against Streptococcus iniae and its in vivo effects on growth, biochemical parameters, innate immunity of rainbow trout (Oncorhynchus mykiss). Therefore, six experimental diets were designed to include different levels of Mooseer from zero (as control), 5, 10, 15, 20, and 25 g per kg diet respectively referred to as M1 to M5. Results from the antibacterial evaluation showed that Mooseer extract inhibits S. iniae growth with MIC and MBC values of 128 and 256 μg ml-1. Appreciable results were obtained in the groups supplemented with Mooseer. Mooseer enhanced growth performance, and modulated serum biochemical and immunological parameters (total protein, albumin, triglyceride, glucose, cortisol, cholesterol, lysozyme, Ig, ACH50, ALP, and protease activity), and liver enzymes (ALT, AST and ALP). The greatest effects were found for higher doses of Mooseer supplementation (M4 and M5). Meanwhile, results from the survival rate of fish challenged with S. iniae showed higher survival in M2 and M4 treatments. The present findings suggest the beneficial use of Mooseer in rainbow trout diet, with 20 g kg-1 inclusion as the recommended dose.",
publisher = "London: Academic Press",
journal = "Fish & Shellfish Immunology",
title = "Mooseer (Allium hirtifolium) boosts growth, general health status, and resistance of rainbow trout (Oncorhynchus mykiss) against Streptococcus iniae infection.",
volume = "120",
doi = "10.1016/j.fsi.2021.12.012",
pages = "360-368"
}

Rashidian, G., Mahboub, H. H., Fahim, A., Hefny, A. A., Prokić, M., Rainis, S., Boldaji, J. T.,& Faggio, C.. (2022). Mooseer (Allium hirtifolium) boosts growth, general health status, and resistance of rainbow trout (Oncorhynchus mykiss) against Streptococcus iniae infection.. in Fish & Shellfish Immunology
London: Academic Press., 120, 360-368.
https://doi.org/10.1016/j.fsi.2021.12.012

Rashidian G, Mahboub HH, Fahim A, Hefny AA, Prokić M, Rainis S, Boldaji JT, Faggio C. Mooseer (Allium hirtifolium) boosts growth, general health status, and resistance of rainbow trout (Oncorhynchus mykiss) against Streptococcus iniae infection.. in Fish & Shellfish Immunology. 2022;120:360-368.
doi:10.1016/j.fsi.2021.12.012 .

Rashidian, Ghasem, Mahboub, Heba H., Fahim, Azin, Hefny, Ahmed A., Prokić, Marko, Rainis, Simona, Boldaji, Javad Tahmasebi, Faggio, Caterina, "Mooseer (Allium hirtifolium) boosts growth, general health status, and resistance of rainbow trout (Oncorhynchus mykiss) against Streptococcus iniae infection." in Fish & Shellfish Immunology, 120 (2022):360-368,
https://doi.org/10.1016/j.fsi.2021.12.012 . .

TY  - JOUR
AU  - Rashidian, Ghasem
AU  - Tahmasebi Boldaji, Javad
AU  - Rainis, Simona
AU  - Prokić, Marko
AU  - Faggio, Caterina
PY  - 2021
UR  - internal-pdf://Rashidian et al. - 2021 - Oregano (Origanum vulgare) Extract Enhances Zebrafish (Danio rerio) Growth Performance, Serum and Mucus Innate.pdf
UR  - https://www.mdpi.com/2076-2615/11/2/299
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4139
AB  - This study evaluated the dietary effects of an ultrasound-assisted extract of Origanum vulgare on the growth, antioxidant and immune responses (serum and mucosal) and resistance of zebrafish (Danio rerio) against Aeromonas hydrophila. Four hundred and forty adult zebrafish were distributed into 12 tanks and fed 4 experimental diets including 0% (control), 0.5% (M1), 1% (M2) and 2% (M3) of the extract per kg−1 diet for eight weeks. Fish were then challenged with A. hydrophila and mortality was recorded for 10 days. Results revealed that the extract exerted potent effects on growth parameters of weight gain and specific growth rate. The feed conversion ratio was significantly lower in fish fed extract-incorporated diets. O. vulgare extract improved antioxidant and immune responses, resulting in less sensitivity to oxidative stress and a higher survival rate when challenged with A. hydrophila. Overall, the greatest effects were observed in individuals with 1% dietary inclusion of the extract. These results suggest that the extract from the plant Origanum vulgare possesses a great potential to be used in the aquaculture industry and that zebrafish is an appropriate model for nutrition studies.
PB  - MDPI AG
T2  - Animals
T1  - Oregano (Origanum vulgare) Extract Enhances Zebrafish (Danio rerio) Growth Performance, Serum and Mucus Innate Immune Responses and Resistance against Aeromonas hydrophila Challenge
IS  - 2
VL  - 11
DO  - 10.3390/ani11020299
SP  - 299
ER  - 

@article{
author = "Rashidian, Ghasem and Tahmasebi Boldaji, Javad and Rainis, Simona and Prokić, Marko and Faggio, Caterina",
year = "2021",
abstract = "This study evaluated the dietary effects of an ultrasound-assisted extract of Origanum vulgare on the growth, antioxidant and immune responses (serum and mucosal) and resistance of zebrafish (Danio rerio) against Aeromonas hydrophila. Four hundred and forty adult zebrafish were distributed into 12 tanks and fed 4 experimental diets including 0% (control), 0.5% (M1), 1% (M2) and 2% (M3) of the extract per kg−1 diet for eight weeks. Fish were then challenged with A. hydrophila and mortality was recorded for 10 days. Results revealed that the extract exerted potent effects on growth parameters of weight gain and specific growth rate. The feed conversion ratio was significantly lower in fish fed extract-incorporated diets. O. vulgare extract improved antioxidant and immune responses, resulting in less sensitivity to oxidative stress and a higher survival rate when challenged with A. hydrophila. Overall, the greatest effects were observed in individuals with 1% dietary inclusion of the extract. These results suggest that the extract from the plant Origanum vulgare possesses a great potential to be used in the aquaculture industry and that zebrafish is an appropriate model for nutrition studies.",
publisher = "MDPI AG",
journal = "Animals",
title = "Oregano (Origanum vulgare) Extract Enhances Zebrafish (Danio rerio) Growth Performance, Serum and Mucus Innate Immune Responses and Resistance against Aeromonas hydrophila Challenge",
number = "2",
volume = "11",
doi = "10.3390/ani11020299",
pages = "299"
}

Rashidian, G., Tahmasebi Boldaji, J., Rainis, S., Prokić, M.,& Faggio, C.. (2021). Oregano (Origanum vulgare) Extract Enhances Zebrafish (Danio rerio) Growth Performance, Serum and Mucus Innate Immune Responses and Resistance against Aeromonas hydrophila Challenge. in Animals
MDPI AG., 11(2), 299.
https://doi.org/10.3390/ani11020299

Rashidian G, Tahmasebi Boldaji J, Rainis S, Prokić M, Faggio C. Oregano (Origanum vulgare) Extract Enhances Zebrafish (Danio rerio) Growth Performance, Serum and Mucus Innate Immune Responses and Resistance against Aeromonas hydrophila Challenge. in Animals. 2021;11(2):299.
doi:10.3390/ani11020299 .

Rashidian, Ghasem, Tahmasebi Boldaji, Javad, Rainis, Simona, Prokić, Marko, Faggio, Caterina, "Oregano (Origanum vulgare) Extract Enhances Zebrafish (Danio rerio) Growth Performance, Serum and Mucus Innate Immune Responses and Resistance against Aeromonas hydrophila Challenge" in Animals, 11, no. 2 (2021):299,
https://doi.org/10.3390/ani11020299 . .

TY  - JOUR
AU  - Prokić, Marko
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Gavrić, Jelena
AU  - Petrović, Tamara
AU  - Despotović, Svetlana
AU  - Faggio, Caterina
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4164
AB  - Although we are witnesses of an increase in the number of studies examining the exposure/effects of microplastics (MPs) on different organisms, there are many unknowns. This review aims to: (i) analyze current studies devoted to investigating the exposure/effects of MPs on animals; (ii) provide some basic knowledge about different model organisms and experimental approaches used in studying MPs; and to (iii) convey directions for future studies. We have summarized data from 500 studies published from January 2011 to May 2020, about different aspects of model organisms (taxonomic group of organisms, type of ecosystem they inhabit, life-stage, sex, tissue and/or organ) and experimental design (laboratory/field, ingestion/bioaccumulation/effect). We also discuss and try to encourage investigation of some less studied organisms (terrestrial and freshwater species, among groups including Annelida, Nematoda, Echinodermata, Cnidaria, Rotifera, birds, amphibians, reptiles), and aspects of MP pollution (long-term field studies, comparative studies examining life stages, sexes, laboratory and field work). We hope that the information presented in this review will serve as a good starting point and will provide useful guidelines for researchers during the process of deciding on the model organism and study designs for investigating MPs.
PB  - Elsevier B.V.
T2  - Journal of Hazardous Materials
T1  - Studying microplastics: Lessons from evaluated literature on animal model organisms and experimental approaches
VL  - 414
DO  - 10.1016/j.jhazmat.2021.125476
SP  - 125476
ER  - 

@article{
author = "Prokić, Marko and Gavrilović, Branka and Radovanović, Tijana and Gavrić, Jelena and Petrović, Tamara and Despotović, Svetlana and Faggio, Caterina",
year = "2021",
abstract = "Although we are witnesses of an increase in the number of studies examining the exposure/effects of microplastics (MPs) on different organisms, there are many unknowns. This review aims to: (i) analyze current studies devoted to investigating the exposure/effects of MPs on animals; (ii) provide some basic knowledge about different model organisms and experimental approaches used in studying MPs; and to (iii) convey directions for future studies. We have summarized data from 500 studies published from January 2011 to May 2020, about different aspects of model organisms (taxonomic group of organisms, type of ecosystem they inhabit, life-stage, sex, tissue and/or organ) and experimental design (laboratory/field, ingestion/bioaccumulation/effect). We also discuss and try to encourage investigation of some less studied organisms (terrestrial and freshwater species, among groups including Annelida, Nematoda, Echinodermata, Cnidaria, Rotifera, birds, amphibians, reptiles), and aspects of MP pollution (long-term field studies, comparative studies examining life stages, sexes, laboratory and field work). We hope that the information presented in this review will serve as a good starting point and will provide useful guidelines for researchers during the process of deciding on the model organism and study designs for investigating MPs.",
publisher = "Elsevier B.V.",
journal = "Journal of Hazardous Materials",
title = "Studying microplastics: Lessons from evaluated literature on animal model organisms and experimental approaches",
volume = "414",
doi = "10.1016/j.jhazmat.2021.125476",
pages = "125476"
}

Prokić, M., Gavrilović, B., Radovanović, T., Gavrić, J., Petrović, T., Despotović, S.,& Faggio, C.. (2021). Studying microplastics: Lessons from evaluated literature on animal model organisms and experimental approaches. in Journal of Hazardous Materials
Elsevier B.V.., 414, 125476.
https://doi.org/10.1016/j.jhazmat.2021.125476

Prokić M, Gavrilović B, Radovanović T, Gavrić J, Petrović T, Despotović S, Faggio C. Studying microplastics: Lessons from evaluated literature on animal model organisms and experimental approaches. in Journal of Hazardous Materials. 2021;414:125476.
doi:10.1016/j.jhazmat.2021.125476 .

Prokić, Marko, Gavrilović, Branka, Radovanović, Tijana, Gavrić, Jelena, Petrović, Tamara, Despotović, Svetlana, Faggio, Caterina, "Studying microplastics: Lessons from evaluated literature on animal model organisms and experimental approaches" in Journal of Hazardous Materials, 414 (2021):125476,
https://doi.org/10.1016/j.jhazmat.2021.125476 . .

TY  - JOUR
AU  - Prokić, Marko
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Gavrić, Jelena
AU  - Petrović, Tamara
AU  - Despotović, Svetlana
AU  - Faggio, Caterina
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4164
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4165
AB  - Although we are witnesses of an increase in the number of studies examining the exposure/effects of microplastics (MPs) on different organisms, there are many unknowns. This review aims to: (i) analyze current studies devoted to investigating the exposure/effects of MPs on animals; (ii) provide some basic knowledge about different model organisms and experimental approaches used in studying MPs; and to (iii) convey directions for future studies. We have summarized data from 500 studies published from January 2011 to May 2020, about different aspects of model organisms (taxonomic group of organisms, type of ecosystem they inhabit, life-stage, sex, tissue and/or organ) and experimental design (laboratory/field, ingestion/bioaccumulation/effect). We also discuss and try to encourage investigation of some less studied organisms (terrestrial and freshwater species, among groups including Annelida, Nematoda, Echinodermata, Cnidaria, Rotifera, birds, amphibians, reptiles), and aspects of MP pollution (long-term field studies, comparative studies examining life stages, sexes, laboratory and field work). We hope that the information presented in this review will serve as a good starting point and will provide useful guidelines for researchers during the process of deciding on the model organism and study designs for investigating MPs.
PB  - Elsevier B.V.
T2  - Journal of Hazardous Materials
T1  - Studying microplastics: Lessons from evaluated literature on animal model organisms and experimental approaches
VL  - 414
DO  - 10.1016/j.jhazmat.2021.125476
SP  - 125476
ER  - 

@article{
author = "Prokić, Marko and Gavrilović, Branka and Radovanović, Tijana and Gavrić, Jelena and Petrović, Tamara and Despotović, Svetlana and Faggio, Caterina",
year = "2021",
abstract = "Although we are witnesses of an increase in the number of studies examining the exposure/effects of microplastics (MPs) on different organisms, there are many unknowns. This review aims to: (i) analyze current studies devoted to investigating the exposure/effects of MPs on animals; (ii) provide some basic knowledge about different model organisms and experimental approaches used in studying MPs; and to (iii) convey directions for future studies. We have summarized data from 500 studies published from January 2011 to May 2020, about different aspects of model organisms (taxonomic group of organisms, type of ecosystem they inhabit, life-stage, sex, tissue and/or organ) and experimental design (laboratory/field, ingestion/bioaccumulation/effect). We also discuss and try to encourage investigation of some less studied organisms (terrestrial and freshwater species, among groups including Annelida, Nematoda, Echinodermata, Cnidaria, Rotifera, birds, amphibians, reptiles), and aspects of MP pollution (long-term field studies, comparative studies examining life stages, sexes, laboratory and field work). We hope that the information presented in this review will serve as a good starting point and will provide useful guidelines for researchers during the process of deciding on the model organism and study designs for investigating MPs.",
publisher = "Elsevier B.V.",
journal = "Journal of Hazardous Materials",
title = "Studying microplastics: Lessons from evaluated literature on animal model organisms and experimental approaches",
volume = "414",
doi = "10.1016/j.jhazmat.2021.125476",
pages = "125476"
}

Prokić, M., Gavrilović, B., Radovanović, T., Gavrić, J., Petrović, T., Despotović, S.,& Faggio, C.. (2021). Studying microplastics: Lessons from evaluated literature on animal model organisms and experimental approaches. in Journal of Hazardous Materials
Elsevier B.V.., 414, 125476.
https://doi.org/10.1016/j.jhazmat.2021.125476

Prokić M, Gavrilović B, Radovanović T, Gavrić J, Petrović T, Despotović S, Faggio C. Studying microplastics: Lessons from evaluated literature on animal model organisms and experimental approaches. in Journal of Hazardous Materials. 2021;414:125476.
doi:10.1016/j.jhazmat.2021.125476 .

Prokić, Marko, Gavrilović, Branka, Radovanović, Tijana, Gavrić, Jelena, Petrović, Tamara, Despotović, Svetlana, Faggio, Caterina, "Studying microplastics: Lessons from evaluated literature on animal model organisms and experimental approaches" in Journal of Hazardous Materials, 414 (2021):125476,
https://doi.org/10.1016/j.jhazmat.2021.125476 . .

TY  - JOUR
AU  - Petrović, Tamara
AU  - Kijanović, Ana
AU  - Tomašević Kolarov, Nataša
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Vukov, Tanja
AU  - Faggio, Caterina
AU  - Prokić, Marko
PY  - 2021
UR  - https://www.mdpi.com/2076-2615/11/4/953
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4192
AB  - In this paper, we examined how the oxidative status (antioxidant system and oxidative damage) of Bombina variegata larvae changed during the metamorphic climax (Gosner stages: 42—beginning, 44—middle and 46—end) and compared the patterns and levels of oxidative stress parameters between individuals developing under constant water availability (control) and those developing under decreasing water availability (desiccation group). Our results revealed that larvae developing under decreasing water availability exhibited increased oxidative damage in the middle and end stages. This was followed by lower levels of glutathione in stages 44 and 46, as well as lower values of catalase, glutathione peroxidase, glutathione S-transferase and sulfhydryl groups in stage 46 (all in relation to control animals). Comparison between stages 42, 44 and 46 within treatments showed that individuals in the last stage demonstrated the highest intensities of lipid oxidative damage in both the control and desiccation groups. As for the parameters of the antioxidant system, control individuals displayed greater variety in response to changes induced by metamorphic climax than individuals exposed to desiccation treatment. The overall decrease in water availability during development led to increased oxidative stress and modifications in the pattern of AOS response to changes induced by metamorphic climax in larvae of B. variegata.
PB  - MDPI AG
T2  - Animals
T1  - Effects of Desiccation on Metamorphic Climax in Bombina variegata: Changes in Levels and Patterns of Oxidative Stress Parameters
IS  - 4
VL  - 11
DO  - 10.3390/ani11040953
SP  - 953
ER  - 

@article{
author = "Petrović, Tamara and Kijanović, Ana and Tomašević Kolarov, Nataša and Gavrić, Jelena and Despotović, Svetlana and Gavrilović, Branka and Radovanović, Tijana and Vukov, Tanja and Faggio, Caterina and Prokić, Marko",
year = "2021",
abstract = "In this paper, we examined how the oxidative status (antioxidant system and oxidative damage) of Bombina variegata larvae changed during the metamorphic climax (Gosner stages: 42—beginning, 44—middle and 46—end) and compared the patterns and levels of oxidative stress parameters between individuals developing under constant water availability (control) and those developing under decreasing water availability (desiccation group). Our results revealed that larvae developing under decreasing water availability exhibited increased oxidative damage in the middle and end stages. This was followed by lower levels of glutathione in stages 44 and 46, as well as lower values of catalase, glutathione peroxidase, glutathione S-transferase and sulfhydryl groups in stage 46 (all in relation to control animals). Comparison between stages 42, 44 and 46 within treatments showed that individuals in the last stage demonstrated the highest intensities of lipid oxidative damage in both the control and desiccation groups. As for the parameters of the antioxidant system, control individuals displayed greater variety in response to changes induced by metamorphic climax than individuals exposed to desiccation treatment. The overall decrease in water availability during development led to increased oxidative stress and modifications in the pattern of AOS response to changes induced by metamorphic climax in larvae of B. variegata.",
publisher = "MDPI AG",
journal = "Animals",
title = "Effects of Desiccation on Metamorphic Climax in Bombina variegata: Changes in Levels and Patterns of Oxidative Stress Parameters",
number = "4",
volume = "11",
doi = "10.3390/ani11040953",
pages = "953"
}

Petrović, T., Kijanović, A., Tomašević Kolarov, N., Gavrić, J., Despotović, S., Gavrilović, B., Radovanović, T., Vukov, T., Faggio, C.,& Prokić, M.. (2021). Effects of Desiccation on Metamorphic Climax in Bombina variegata: Changes in Levels and Patterns of Oxidative Stress Parameters. in Animals
MDPI AG., 11(4), 953.
https://doi.org/10.3390/ani11040953

Petrović T, Kijanović A, Tomašević Kolarov N, Gavrić J, Despotović S, Gavrilović B, Radovanović T, Vukov T, Faggio C, Prokić M. Effects of Desiccation on Metamorphic Climax in Bombina variegata: Changes in Levels and Patterns of Oxidative Stress Parameters. in Animals. 2021;11(4):953.
doi:10.3390/ani11040953 .

Petrović, Tamara, Kijanović, Ana, Tomašević Kolarov, Nataša, Gavrić, Jelena, Despotović, Svetlana, Gavrilović, Branka, Radovanović, Tijana, Vukov, Tanja, Faggio, Caterina, Prokić, Marko, "Effects of Desiccation on Metamorphic Climax in Bombina variegata: Changes in Levels and Patterns of Oxidative Stress Parameters" in Animals, 11, no. 4 (2021):953,
https://doi.org/10.3390/ani11040953 . .

TY  - JOUR
AU  - Yalsuyi, Ahmad Mohamadi
AU  - Hajimoradloo, Abdolmajid
AU  - Ghorbani, Rasul
AU  - Jafari, Vally-allah
AU  - Prokić, Marko
AU  - Faggio, Caterina
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4215
AB  - In the study, we assessed how acute changes in water temperature and dissolved ammonia concentration can affect the swimming behavior pattern of rainbow trout (Oncorhynchus mykiss). The behavior was analyzed in three different stages: 1) increase stage, (where temperature and ammonia concentration increase during this step, respectively); 2) unchanged stage, (where levels of both factors do not change during this stage); 3) reduction stage, (where ammonia concentration and temperature during this stage are reduced), respectively. The results showed that both factors significantly changed the swimming pattern of the rainbow trout. There were significant differences in swimming parameters (distance from the center, swimming speed, total movement and the average of angular changes of movement) of treated fish in the comparison between treatments, and with the control group. The changes in the swimming pattern of fish in response to physicochemical parameters of water were confirmed to be a good tool in ecotoxicological studies.
PB  - Elsevier BV
T2  - Environmental Toxicology and Pharmacology
T1  - Behavior evaluation of rainbow trout (Oncorhynchus mykiss) following temperature and ammonia alterations.
VL  - 86
DO  - 10.1016/j.etap.2021.103648
SP  - 103648
ER  - 

@article{
author = "Yalsuyi, Ahmad Mohamadi and Hajimoradloo, Abdolmajid and Ghorbani, Rasul and Jafari, Vally-allah and Prokić, Marko and Faggio, Caterina",
year = "2021",
abstract = "In the study, we assessed how acute changes in water temperature and dissolved ammonia concentration can affect the swimming behavior pattern of rainbow trout (Oncorhynchus mykiss). The behavior was analyzed in three different stages: 1) increase stage, (where temperature and ammonia concentration increase during this step, respectively); 2) unchanged stage, (where levels of both factors do not change during this stage); 3) reduction stage, (where ammonia concentration and temperature during this stage are reduced), respectively. The results showed that both factors significantly changed the swimming pattern of the rainbow trout. There were significant differences in swimming parameters (distance from the center, swimming speed, total movement and the average of angular changes of movement) of treated fish in the comparison between treatments, and with the control group. The changes in the swimming pattern of fish in response to physicochemical parameters of water were confirmed to be a good tool in ecotoxicological studies.",
publisher = "Elsevier BV",
journal = "Environmental Toxicology and Pharmacology",
title = "Behavior evaluation of rainbow trout (Oncorhynchus mykiss) following temperature and ammonia alterations.",
volume = "86",
doi = "10.1016/j.etap.2021.103648",
pages = "103648"
}

Yalsuyi, A. M., Hajimoradloo, A., Ghorbani, R., Jafari, V., Prokić, M.,& Faggio, C.. (2021). Behavior evaluation of rainbow trout (Oncorhynchus mykiss) following temperature and ammonia alterations.. in Environmental Toxicology and Pharmacology
Elsevier BV., 86, 103648.
https://doi.org/10.1016/j.etap.2021.103648

Yalsuyi AM, Hajimoradloo A, Ghorbani R, Jafari V, Prokić M, Faggio C. Behavior evaluation of rainbow trout (Oncorhynchus mykiss) following temperature and ammonia alterations.. in Environmental Toxicology and Pharmacology. 2021;86:103648.
doi:10.1016/j.etap.2021.103648 .

Yalsuyi, Ahmad Mohamadi, Hajimoradloo, Abdolmajid, Ghorbani, Rasul, Jafari, Vally-allah, Prokić, Marko, Faggio, Caterina, "Behavior evaluation of rainbow trout (Oncorhynchus mykiss) following temperature and ammonia alterations." in Environmental Toxicology and Pharmacology, 86 (2021):103648,
https://doi.org/10.1016/j.etap.2021.103648 . .

TY  - JOUR
AU  - Vajargah, Mohammad Forouhar
AU  - Namin, Javid Imanpour
AU  - Mohsenpour, Reza
AU  - Yalsuyi, Ahmad Mohammadi
AU  - Prokić, Marko
AU  - Faggio, Caterina
PY  - 2021
UR  - https://link.springer.com/10.1007/s11259-021-09818-y
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4458
AB  - This study investigates acute toxicity and histological effects of Lindane insecticide on intestinal tissues of immature grass carp (Ctenopharyngodon idella). For these purposes, 105 fish were exposed to different concentrations of Lindane for 96 h in 5 groups with 3 replications. Samples of intestinal tissues were prepared in both control and exposure groups during and after the toxicity test. Vaculation, necrosis, bleeding and epithelial degeneration were apparent in the intestine tissue of treated individuals. The LC50 96 h of Lindane was 0.788 ppm in the present study, also the results showed besides pesticide-induced lesions, symptoms of abnormal swimming, anxiety, tendency to swim near the surface, and death due to terminal toxicity with mouth widely open. The exposed fish were pale in color, showed oxygen deficit and severe respiratory problems. Overall, our results confirm the toxicity of different concentrations of Lindane insecticide affecting behavior, intestinal function and inducing tissue lesions of immature grass carp.
PB  - Dordrecht: Springer Science and Business Media B.V.
T2  - Veterinary Research Communications
T1  - Histological effects of sublethal concentrations of insecticide Lindane on intestinal tissue of grass carp (Ctenopharyngodon idella).
DO  - 10.1007/s11259-021-09818-y
ER  - 

@article{
author = "Vajargah, Mohammad Forouhar and Namin, Javid Imanpour and Mohsenpour, Reza and Yalsuyi, Ahmad Mohammadi and Prokić, Marko and Faggio, Caterina",
year = "2021",
abstract = "This study investigates acute toxicity and histological effects of Lindane insecticide on intestinal tissues of immature grass carp (Ctenopharyngodon idella). For these purposes, 105 fish were exposed to different concentrations of Lindane for 96 h in 5 groups with 3 replications. Samples of intestinal tissues were prepared in both control and exposure groups during and after the toxicity test. Vaculation, necrosis, bleeding and epithelial degeneration were apparent in the intestine tissue of treated individuals. The LC50 96 h of Lindane was 0.788 ppm in the present study, also the results showed besides pesticide-induced lesions, symptoms of abnormal swimming, anxiety, tendency to swim near the surface, and death due to terminal toxicity with mouth widely open. The exposed fish were pale in color, showed oxygen deficit and severe respiratory problems. Overall, our results confirm the toxicity of different concentrations of Lindane insecticide affecting behavior, intestinal function and inducing tissue lesions of immature grass carp.",
publisher = "Dordrecht: Springer Science and Business Media B.V.",
journal = "Veterinary Research Communications",
title = "Histological effects of sublethal concentrations of insecticide Lindane on intestinal tissue of grass carp (Ctenopharyngodon idella).",
doi = "10.1007/s11259-021-09818-y"
}

Vajargah, M. F., Namin, J. I., Mohsenpour, R., Yalsuyi, A. M., Prokić, M.,& Faggio, C.. (2021). Histological effects of sublethal concentrations of insecticide Lindane on intestinal tissue of grass carp (Ctenopharyngodon idella).. in Veterinary Research Communications
Dordrecht: Springer Science and Business Media B.V...
https://doi.org/10.1007/s11259-021-09818-y

Vajargah MF, Namin JI, Mohsenpour R, Yalsuyi AM, Prokić M, Faggio C. Histological effects of sublethal concentrations of insecticide Lindane on intestinal tissue of grass carp (Ctenopharyngodon idella).. in Veterinary Research Communications. 2021;.
doi:10.1007/s11259-021-09818-y .

Vajargah, Mohammad Forouhar, Namin, Javid Imanpour, Mohsenpour, Reza, Yalsuyi, Ahmad Mohammadi, Prokić, Marko, Faggio, Caterina, "Histological effects of sublethal concentrations of insecticide Lindane on intestinal tissue of grass carp (Ctenopharyngodon idella)." in Veterinary Research Communications (2021),
https://doi.org/10.1007/s11259-021-09818-y . .

TY  - JOUR
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Petrović, Tamara
AU  - Ajduković, Maja
AU  - Vučić, Tijana
AU  - Cvijanović, Milena
AU  - Faggio, Caterina
AU  - Prokić, Marko
PY  - 2021
UR  - https://www.mdpi.com/1660-4601/18/18/9653
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8464833
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4483
AB  - Thiourea is an established disruptor of thyroid hormone synthesis and is frequently used as an inhibitor of metamorphosis. The changes caused by thiourea can affect processes associated with the oxidative status of individuals (metabolic rate, the HPI axis, antioxidant system). We investigated the parameters of oxidative stress in crested newt (Triturus spp.) larvae during normal development in late larval stage 62 and newly metamorphosed individuals, and during thiourea-stimulated metamorphosis arrest in individuals exposed to low (0.05%) and high (0.1%) concentrations of thiourea. Both groups of crested newts exposed to thiourea retained their larval characteristics until the end of the experiment. The low activities of antioxidant enzymes and the high lipid peroxidation level pointed to increased oxidative stress in larvae at the beginning of stage 62 as compared to fully metamorphosed individuals. The activities of catalase (CAT) and glutathione-S-transferase (GST) and the concentration of sulfhydryl (SH) groups were significantly lower in larvae reared in aqueous solutions containing thiourea than in newly metamorphosed individuals. The high thiourea concentration (0.1%) affected the antioxidative parameters to the extent that oxidative damage could not be avoided, contrary to a lower concentration. Our results provide a first insight into the physiological adaptations of crested newts during normal development and simulated metamorphosis arrest.
PB  - Basel: MDPI
T2  - International Journal of Environmental Research and Public Health
T1  - Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis.
IS  - 18
VL  - 18
DO  - 10.3390/ijerph18189653
SP  - 9653
ER  - 

@article{
author = "Gavrić, Jelena and Despotović, Svetlana and Gavrilović, Branka and Radovanović, Tijana and Petrović, Tamara and Ajduković, Maja and Vučić, Tijana and Cvijanović, Milena and Faggio, Caterina and Prokić, Marko",
year = "2021",
abstract = "Thiourea is an established disruptor of thyroid hormone synthesis and is frequently used as an inhibitor of metamorphosis. The changes caused by thiourea can affect processes associated with the oxidative status of individuals (metabolic rate, the HPI axis, antioxidant system). We investigated the parameters of oxidative stress in crested newt (Triturus spp.) larvae during normal development in late larval stage 62 and newly metamorphosed individuals, and during thiourea-stimulated metamorphosis arrest in individuals exposed to low (0.05%) and high (0.1%) concentrations of thiourea. Both groups of crested newts exposed to thiourea retained their larval characteristics until the end of the experiment. The low activities of antioxidant enzymes and the high lipid peroxidation level pointed to increased oxidative stress in larvae at the beginning of stage 62 as compared to fully metamorphosed individuals. The activities of catalase (CAT) and glutathione-S-transferase (GST) and the concentration of sulfhydryl (SH) groups were significantly lower in larvae reared in aqueous solutions containing thiourea than in newly metamorphosed individuals. The high thiourea concentration (0.1%) affected the antioxidative parameters to the extent that oxidative damage could not be avoided, contrary to a lower concentration. Our results provide a first insight into the physiological adaptations of crested newts during normal development and simulated metamorphosis arrest.",
publisher = "Basel: MDPI",
journal = "International Journal of Environmental Research and Public Health",
title = "Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis.",
number = "18",
volume = "18",
doi = "10.3390/ijerph18189653",
pages = "9653"
}

Gavrić, J., Despotović, S., Gavrilović, B., Radovanović, T., Petrović, T., Ajduković, M., Vučić, T., Cvijanović, M., Faggio, C.,& Prokić, M.. (2021). Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis.. in International Journal of Environmental Research and Public Health
Basel: MDPI., 18(18), 9653.
https://doi.org/10.3390/ijerph18189653

Gavrić J, Despotović S, Gavrilović B, Radovanović T, Petrović T, Ajduković M, Vučić T, Cvijanović M, Faggio C, Prokić M. Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis.. in International Journal of Environmental Research and Public Health. 2021;18(18):9653.
doi:10.3390/ijerph18189653 .

Gavrić, Jelena, Despotović, Svetlana, Gavrilović, Branka, Radovanović, Tijana, Petrović, Tamara, Ajduković, Maja, Vučić, Tijana, Cvijanović, Milena, Faggio, Caterina, Prokić, Marko, "Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis." in International Journal of Environmental Research and Public Health, 18, no. 18 (2021):9653,
https://doi.org/10.3390/ijerph18189653 . .

TY  - JOUR
AU  - Radovanović, Tijana
AU  - Gavrilović, Branka
AU  - Petrović, Tamara
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Kijanović, Ana
AU  - Mirč, Marko
AU  - Tomašević Kolarov, Nataša
AU  - Faggio, Caterina
AU  - Prokić, Marko
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S1532045621002180
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4484
AB  - Global warming represents a severe threat to existing ecosystems, especially for anuran tadpoles who encounter significant fluctuations in their habitats. Decreasing water levels in permanent and temporary water bodies is a significant risk for larval survival or fitness. On the other hand, the natural environment of amphibians is extremely polluted by various xenobiotics. This study evaluated how pre-exposure of Bombina variegata tadpoles to chronic environmental stress (desiccation) modulates the biochemical response of juvenile individuals to following acute chemical stressor (pesticide deltamethrin). Our results demonstrated that individually applied pesticide changed the thiol and lipid status of the treated juveniles but animals subjected solely to desiccation pressure were more tolerant to free radicals and showed no induction of lipid peroxidation. Comparison of juveniles exposed to deltamethrin revealed that desiccation pretreatment during the larval stage of development modified cellular protection in the juveniles. Higher activities of CAT, GSH-Px and GR were recorded in the pre-exposed group, as well as a lower degree of lipid peroxidation relative to the group that was not pre-exposed to low water stress. Pre-desiccated groups displayed the greatest range of coordination of investigated antioxidant parameters, supported by Pearson's correlations. Activation of the GSH-redox system is a significant marker in juveniles against stress caused by desiccation and a chemical stressor. The stressful environment experienced during tadpole development produced an adaptive reaction to subsequent exposure to another stressor in juveniles. To develop relevant management and conservation strategies, more studies of the interactive effects of environmental and chemical stressors are necessary.
PB  - New York: Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.
VL  - 250
DO  - 10.1016/j.cbpc.2021.109191
SP  - 109191
ER  - 

@article{
author = "Radovanović, Tijana and Gavrilović, Branka and Petrović, Tamara and Despotović, Svetlana and Gavrić, Jelena and Kijanović, Ana and Mirč, Marko and Tomašević Kolarov, Nataša and Faggio, Caterina and Prokić, Marko",
year = "2021",
abstract = "Global warming represents a severe threat to existing ecosystems, especially for anuran tadpoles who encounter significant fluctuations in their habitats. Decreasing water levels in permanent and temporary water bodies is a significant risk for larval survival or fitness. On the other hand, the natural environment of amphibians is extremely polluted by various xenobiotics. This study evaluated how pre-exposure of Bombina variegata tadpoles to chronic environmental stress (desiccation) modulates the biochemical response of juvenile individuals to following acute chemical stressor (pesticide deltamethrin). Our results demonstrated that individually applied pesticide changed the thiol and lipid status of the treated juveniles but animals subjected solely to desiccation pressure were more tolerant to free radicals and showed no induction of lipid peroxidation. Comparison of juveniles exposed to deltamethrin revealed that desiccation pretreatment during the larval stage of development modified cellular protection in the juveniles. Higher activities of CAT, GSH-Px and GR were recorded in the pre-exposed group, as well as a lower degree of lipid peroxidation relative to the group that was not pre-exposed to low water stress. Pre-desiccated groups displayed the greatest range of coordination of investigated antioxidant parameters, supported by Pearson's correlations. Activation of the GSH-redox system is a significant marker in juveniles against stress caused by desiccation and a chemical stressor. The stressful environment experienced during tadpole development produced an adaptive reaction to subsequent exposure to another stressor in juveniles. To develop relevant management and conservation strategies, more studies of the interactive effects of environmental and chemical stressors are necessary.",
publisher = "New York: Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.",
volume = "250",
doi = "10.1016/j.cbpc.2021.109191",
pages = "109191"
}

Radovanović, T., Gavrilović, B., Petrović, T., Despotović, S., Gavrić, J., Kijanović, A., Mirč, M., Tomašević Kolarov, N., Faggio, C.,& Prokić, M.. (2021). Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
New York: Elsevier Inc.., 250, 109191.
https://doi.org/10.1016/j.cbpc.2021.109191

Radovanović T, Gavrilović B, Petrović T, Despotović S, Gavrić J, Kijanović A, Mirč M, Tomašević Kolarov N, Faggio C, Prokić M. Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2021;250:109191.
doi:10.1016/j.cbpc.2021.109191 .

Radovanović, Tijana, Gavrilović, Branka, Petrović, Tamara, Despotović, Svetlana, Gavrić, Jelena, Kijanović, Ana, Mirč, Marko, Tomašević Kolarov, Nataša, Faggio, Caterina, Prokić, Marko, "Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles." in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 250 (2021):109191,
https://doi.org/10.1016/j.cbpc.2021.109191 . .

TY  - JOUR
AU  - Radovanović, Tijana
AU  - Gavrilović, Branka
AU  - Petrović, Tamara
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Kijanović, Ana
AU  - Mirč, Marko
AU  - Tomašević Kolarov, Nataša
AU  - Faggio, Caterina
AU  - Prokić, Marko
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S1532045621002180
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4484
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4497
AB  - Global warming represents a severe threat to existing ecosystems, especially for anuran tadpoles who encounter significant fluctuations in their habitats. Decreasing water levels in permanent and temporary water bodies is a significant risk for larval survival or fitness. On the other hand, the natural environment of amphibians is extremely polluted by various xenobiotics. This study evaluated how pre-exposure of Bombina variegata tadpoles to chronic environmental stress (desiccation) modulates the biochemical response of juvenile individuals to following acute chemical stressor (pesticide deltamethrin). Our results demonstrated that individually applied pesticide changed the thiol and lipid status of the treated juveniles but animals subjected solely to desiccation pressure were more tolerant to free radicals and showed no induction of lipid peroxidation. Comparison of juveniles exposed to deltamethrin revealed that desiccation pretreatment during the larval stage of development modified cellular protection in the juveniles. Higher activities of CAT, GSH-Px and GR were recorded in the pre-exposed group, as well as a lower degree of lipid peroxidation relative to the group that was not pre-exposed to low water stress. Pre-desiccated groups displayed the greatest range of coordination of investigated antioxidant parameters, supported by Pearson's correlations. Activation of the GSH-redox system is a significant marker in juveniles against stress caused by desiccation and a chemical stressor. The stressful environment experienced during tadpole development produced an adaptive reaction to subsequent exposure to another stressor in juveniles. To develop relevant management and conservation strategies, more studies of the interactive effects of environmental and chemical stressors are necessary.
PB  - New York: Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.
VL  - 250
DO  - 10.1016/j.cbpc.2021.109191
SP  - 109191
ER  - 

@article{
author = "Radovanović, Tijana and Gavrilović, Branka and Petrović, Tamara and Despotović, Svetlana and Gavrić, Jelena and Kijanović, Ana and Mirč, Marko and Tomašević Kolarov, Nataša and Faggio, Caterina and Prokić, Marko",
year = "2021",
abstract = "Global warming represents a severe threat to existing ecosystems, especially for anuran tadpoles who encounter significant fluctuations in their habitats. Decreasing water levels in permanent and temporary water bodies is a significant risk for larval survival or fitness. On the other hand, the natural environment of amphibians is extremely polluted by various xenobiotics. This study evaluated how pre-exposure of Bombina variegata tadpoles to chronic environmental stress (desiccation) modulates the biochemical response of juvenile individuals to following acute chemical stressor (pesticide deltamethrin). Our results demonstrated that individually applied pesticide changed the thiol and lipid status of the treated juveniles but animals subjected solely to desiccation pressure were more tolerant to free radicals and showed no induction of lipid peroxidation. Comparison of juveniles exposed to deltamethrin revealed that desiccation pretreatment during the larval stage of development modified cellular protection in the juveniles. Higher activities of CAT, GSH-Px and GR were recorded in the pre-exposed group, as well as a lower degree of lipid peroxidation relative to the group that was not pre-exposed to low water stress. Pre-desiccated groups displayed the greatest range of coordination of investigated antioxidant parameters, supported by Pearson's correlations. Activation of the GSH-redox system is a significant marker in juveniles against stress caused by desiccation and a chemical stressor. The stressful environment experienced during tadpole development produced an adaptive reaction to subsequent exposure to another stressor in juveniles. To develop relevant management and conservation strategies, more studies of the interactive effects of environmental and chemical stressors are necessary.",
publisher = "New York: Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.",
volume = "250",
doi = "10.1016/j.cbpc.2021.109191",
pages = "109191"
}

Radovanović, T., Gavrilović, B., Petrović, T., Despotović, S., Gavrić, J., Kijanović, A., Mirč, M., Tomašević Kolarov, N., Faggio, C.,& Prokić, M.. (2021). Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
New York: Elsevier Inc.., 250, 109191.
https://doi.org/10.1016/j.cbpc.2021.109191

Radovanović T, Gavrilović B, Petrović T, Despotović S, Gavrić J, Kijanović A, Mirč M, Tomašević Kolarov N, Faggio C, Prokić M. Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2021;250:109191.
doi:10.1016/j.cbpc.2021.109191 .

Radovanović, Tijana, Gavrilović, Branka, Petrović, Tamara, Despotović, Svetlana, Gavrić, Jelena, Kijanović, Ana, Mirč, Marko, Tomašević Kolarov, Nataša, Faggio, Caterina, Prokić, Marko, "Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles." in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 250 (2021):109191,
https://doi.org/10.1016/j.cbpc.2021.109191 . .

TY  - JOUR
AU  - Yalsuyi, Ahmad Mohamadi
AU  - Vajargah, Mohammad Forouhar
AU  - Hajimoradloo, Abdolmajid
AU  - Galangash, Mohsen Mohammadi
AU  - Prokić, Marko
AU  - Faggio, Caterina
PY  - 2021
UR  - https://www.mdpi.com/2306-7381/8/10/218
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8540590
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4633
AB  - Pesticides can induce changes in behavior and reduce the survival chance of aquatic organisms. In this study, the toxic effects of glyphosate suspension (Glyphosate Aria 41% SL, Tehran Iran) on behavior and tissues of common carp (Cyprinus carpio) were assessed. For this purpose, a 96 h LC50 of glyphosate suspension (68.788 mL·L-1) was used in the toxicity test. All individuals were divided into control and treatment groups with four replicates. Exposure operations were performed under two conditions: increasing concentration of suspension from 0 to 68.788 mL·L-1; then, decreasing to the first level. The swimming pattern was recorded by digital cameras during the test and tissue samples were collected at the end of the test. There were significant differences between the swimming pattern of treated individuals and control ones during both steps. The sublethal concentration of glyphosate led to hypertrophy, hyperplasia and hyperemia in the gill of fish. However, changes were obvious only after sampling. The exposed fish also displayed clinical signs such as darkening of the skin and increasing movement of the operculum. Moreover, glyphosate suspension affected swimming patterns of fish suggest that the swimming behavior test can indicate the potential toxicity of environmental pollutants and be used as a noninvasive, useful method for managing environmental changes and assessing fish health conditions by video monitoring.
PB  - Basel: MDPI
T2  - Veterinary Sciences
T1  - Evaluation of Behavioral Changes and Tissue Damages in Common Carp (Cyprinus carpio) after Exposure to the Herbicide Glyphosate.
IS  - 10
VL  - 8
DO  - 10.3390/vetsci8100218
SP  - 218
ER  - 

@article{
author = "Yalsuyi, Ahmad Mohamadi and Vajargah, Mohammad Forouhar and Hajimoradloo, Abdolmajid and Galangash, Mohsen Mohammadi and Prokić, Marko and Faggio, Caterina",
year = "2021",
abstract = "Pesticides can induce changes in behavior and reduce the survival chance of aquatic organisms. In this study, the toxic effects of glyphosate suspension (Glyphosate Aria 41% SL, Tehran Iran) on behavior and tissues of common carp (Cyprinus carpio) were assessed. For this purpose, a 96 h LC50 of glyphosate suspension (68.788 mL·L-1) was used in the toxicity test. All individuals were divided into control and treatment groups with four replicates. Exposure operations were performed under two conditions: increasing concentration of suspension from 0 to 68.788 mL·L-1; then, decreasing to the first level. The swimming pattern was recorded by digital cameras during the test and tissue samples were collected at the end of the test. There were significant differences between the swimming pattern of treated individuals and control ones during both steps. The sublethal concentration of glyphosate led to hypertrophy, hyperplasia and hyperemia in the gill of fish. However, changes were obvious only after sampling. The exposed fish also displayed clinical signs such as darkening of the skin and increasing movement of the operculum. Moreover, glyphosate suspension affected swimming patterns of fish suggest that the swimming behavior test can indicate the potential toxicity of environmental pollutants and be used as a noninvasive, useful method for managing environmental changes and assessing fish health conditions by video monitoring.",
publisher = "Basel: MDPI",
journal = "Veterinary Sciences",
title = "Evaluation of Behavioral Changes and Tissue Damages in Common Carp (Cyprinus carpio) after Exposure to the Herbicide Glyphosate.",
number = "10",
volume = "8",
doi = "10.3390/vetsci8100218",
pages = "218"
}

Yalsuyi, A. M., Vajargah, M. F., Hajimoradloo, A., Galangash, M. M., Prokić, M.,& Faggio, C.. (2021). Evaluation of Behavioral Changes and Tissue Damages in Common Carp (Cyprinus carpio) after Exposure to the Herbicide Glyphosate.. in Veterinary Sciences
Basel: MDPI., 8(10), 218.
https://doi.org/10.3390/vetsci8100218

Yalsuyi AM, Vajargah MF, Hajimoradloo A, Galangash MM, Prokić M, Faggio C. Evaluation of Behavioral Changes and Tissue Damages in Common Carp (Cyprinus carpio) after Exposure to the Herbicide Glyphosate.. in Veterinary Sciences. 2021;8(10):218.
doi:10.3390/vetsci8100218 .

Yalsuyi, Ahmad Mohamadi, Vajargah, Mohammad Forouhar, Hajimoradloo, Abdolmajid, Galangash, Mohsen Mohammadi, Prokić, Marko, Faggio, Caterina, "Evaluation of Behavioral Changes and Tissue Damages in Common Carp (Cyprinus carpio) after Exposure to the Herbicide Glyphosate." in Veterinary Sciences, 8, no. 10 (2021):218,
https://doi.org/10.3390/vetsci8100218 . .

TY  - JOUR
AU  - Mohamadi Yalsuyi, Ahmad
AU  - Forouhar Vajargah, Mohammad
AU  - Hajimoradloo, Abdolmajid
AU  - Mohammadi Galangash, Mohsen
AU  - Prokić, Marko
AU  - Faggio, Caterina
PY  - 2021
UR  - https://link.springer.com/10.1007/s11259-021-09862-8
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4691
AB  - Industrial chemical solutions are widely used as a method to disinfection of aquaculture water and environments. The aim of the present study was to evaluate the toxicity effect of Betadine (10% solution of povidone-iodine) as a disinfectant solution on the survival and gill tissue of Oranda goldfish (Carassius auratus). For these purposes, 225 fingerling Oranda goldfish with an average weight 5 ± 0.67 g were divided into 15 groups with 3 replications. Fish were exposed to series of concentrations (0, 10, 20, 40, 60, 80, 90, 100, 120, 140, 160, 180, 200, 220 and 240 mg/L) of Betadine for 96 h. The mortality of fish and the samples of gill were observed at 6, 12, 18, 24, 48, 72 and 96 h after exposure. The results of the present study showed that the half-life of Betadine was less than 24 h and mortality were not record after 24 h. The highest mortality rate was at 240 mg/L, and LC50 24 h of Betadine was 158.800 mg/L. Histopathological results showed that lethal concentrations of Betadine lead to hyperemia, hypertrophy, hyperplasia and adhesion of secondary lamellar of the gill. Moreover, fish that were exposed to these concentrations displayed clinical signs such as anxiety, darkening of the skin. Overall results showed that Betadine have short half-life in the aquatic environment and are toxic to fish at very high concentrations, therefore it can be considered as practically non-toxic and useful for disinfection of the aquatic environment.
PB  - Dordrecht: Springer Science and Business Media B.V.
T2  - Veterinary Research Communications
T1  - Can Betadine (10% povidone-iodine solution) act on the survival rate and gill tissue structure of Oranda goldfish (Carassius auratus)?
DO  - 10.1007/s11259-021-09862-8
ER  - 

@article{
author = "Mohamadi Yalsuyi, Ahmad and Forouhar Vajargah, Mohammad and Hajimoradloo, Abdolmajid and Mohammadi Galangash, Mohsen and Prokić, Marko and Faggio, Caterina",
year = "2021",
abstract = "Industrial chemical solutions are widely used as a method to disinfection of aquaculture water and environments. The aim of the present study was to evaluate the toxicity effect of Betadine (10% solution of povidone-iodine) as a disinfectant solution on the survival and gill tissue of Oranda goldfish (Carassius auratus). For these purposes, 225 fingerling Oranda goldfish with an average weight 5 ± 0.67 g were divided into 15 groups with 3 replications. Fish were exposed to series of concentrations (0, 10, 20, 40, 60, 80, 90, 100, 120, 140, 160, 180, 200, 220 and 240 mg/L) of Betadine for 96 h. The mortality of fish and the samples of gill were observed at 6, 12, 18, 24, 48, 72 and 96 h after exposure. The results of the present study showed that the half-life of Betadine was less than 24 h and mortality were not record after 24 h. The highest mortality rate was at 240 mg/L, and LC50 24 h of Betadine was 158.800 mg/L. Histopathological results showed that lethal concentrations of Betadine lead to hyperemia, hypertrophy, hyperplasia and adhesion of secondary lamellar of the gill. Moreover, fish that were exposed to these concentrations displayed clinical signs such as anxiety, darkening of the skin. Overall results showed that Betadine have short half-life in the aquatic environment and are toxic to fish at very high concentrations, therefore it can be considered as practically non-toxic and useful for disinfection of the aquatic environment.",
publisher = "Dordrecht: Springer Science and Business Media B.V.",
journal = "Veterinary Research Communications",
title = "Can Betadine (10% povidone-iodine solution) act on the survival rate and gill tissue structure of Oranda goldfish (Carassius auratus)?",
doi = "10.1007/s11259-021-09862-8"
}

Mohamadi Yalsuyi, A., Forouhar Vajargah, M., Hajimoradloo, A., Mohammadi Galangash, M., Prokić, M.,& Faggio, C.. (2021). Can Betadine (10% povidone-iodine solution) act on the survival rate and gill tissue structure of Oranda goldfish (Carassius auratus)?. in Veterinary Research Communications
Dordrecht: Springer Science and Business Media B.V...
https://doi.org/10.1007/s11259-021-09862-8

Mohamadi Yalsuyi A, Forouhar Vajargah M, Hajimoradloo A, Mohammadi Galangash M, Prokić M, Faggio C. Can Betadine (10% povidone-iodine solution) act on the survival rate and gill tissue structure of Oranda goldfish (Carassius auratus)?. in Veterinary Research Communications. 2021;.
doi:10.1007/s11259-021-09862-8 .

Mohamadi Yalsuyi, Ahmad, Forouhar Vajargah, Mohammad, Hajimoradloo, Abdolmajid, Mohammadi Galangash, Mohsen, Prokić, Marko, Faggio, Caterina, "Can Betadine (10% povidone-iodine solution) act on the survival rate and gill tissue structure of Oranda goldfish (Carassius auratus)?" in Veterinary Research Communications (2021),
https://doi.org/10.1007/s11259-021-09862-8 . .

TY  - JOUR
AU  - Rashidian, Ghasem
AU  - Lazado, Carlo C.
AU  - Mahboub, Heba H.
AU  - Mohammadi-Aloucheh, Ramin
AU  - Prokić, Marko
AU  - Nada, Hend S.
AU  - Faggio, Caterina
PY  - 2021
UR  - https://www.mdpi.com/1422-0067/22/6/3270
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4176
AB  - This study was conducted to compare the effects of commercially available (C) and green synthesized (GS) Zinc oxide nanoparticles (ZnO-NPs) on immunological responses of common carp (Cyprinus carpio) skin mucus. GS ZnO-NPs were generated using Thymus pubescent and characterized by UV–vis diffuse reflectance spectroscopy (DRS), Fourier-transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), scanning electron microscope (SEM), and energy-dispersive X-ray spectroscopy (EDX). Fish (n = 150) were randomly allocated into five groups in triplicate and received a waterborne concentration of 0% (control), 25%, and 50% of LC50 96 h of commercially available (C1 and C2) and green synthesized ZnO-NPs (GS1 and GS2) for 21 days. Results from XRD displayed ZnO-NPs with 58 nm in size and UV-vis DRS, EDX, and FT-IR analysis showed that some functional groups from plant extract bonded to the surface of NPs. The SEM images showed that ZnO-NPs have conical morphology. Acute toxicity study showed a higher dose of LC5096h for green synthesized ZnO-NPs (78.9 mg.L−1) compared to the commercial source (59.95 mg.L−1). The highest activity of lysozyme and alternative complement activity (ACH50) were found in control and GS1 groups. A significant decrease in alkaline phosphatase activity (ALP) was found in C1 and C2 groups compared to other treatments. Protease activity (P) was significantly decreased in the C2 group compared to the control and GS groups. Total immunoglobulin (total Ig) content was the highest in the control. In addition, total Ig in the GS1 group was higher than GS2. The exposure to ZnO-NPs lowered total protein content in all experimental groups when compared to control. Present findings revealed lower induced immunosuppressive effects by green synthesized ZnO-NPs on key parameters of fish skin mucus.
PB  - MDPI AG
T2  - International Journal of Molecular Sciences
T1  - Chemically and Green Synthesized ZnO Nanoparticles Alter Key Immunological Molecules in Common Carp (Cyprinus carpio) Skin Mucus
IS  - 6
VL  - 22
DO  - 10.3390/ijms22063270
SP  - 3270
ER  - 

@article{
author = "Rashidian, Ghasem and Lazado, Carlo C. and Mahboub, Heba H. and Mohammadi-Aloucheh, Ramin and Prokić, Marko and Nada, Hend S. and Faggio, Caterina",
year = "2021",
abstract = "This study was conducted to compare the effects of commercially available (C) and green synthesized (GS) Zinc oxide nanoparticles (ZnO-NPs) on immunological responses of common carp (Cyprinus carpio) skin mucus. GS ZnO-NPs were generated using Thymus pubescent and characterized by UV–vis diffuse reflectance spectroscopy (DRS), Fourier-transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), scanning electron microscope (SEM), and energy-dispersive X-ray spectroscopy (EDX). Fish (n = 150) were randomly allocated into five groups in triplicate and received a waterborne concentration of 0% (control), 25%, and 50% of LC50 96 h of commercially available (C1 and C2) and green synthesized ZnO-NPs (GS1 and GS2) for 21 days. Results from XRD displayed ZnO-NPs with 58 nm in size and UV-vis DRS, EDX, and FT-IR analysis showed that some functional groups from plant extract bonded to the surface of NPs. The SEM images showed that ZnO-NPs have conical morphology. Acute toxicity study showed a higher dose of LC5096h for green synthesized ZnO-NPs (78.9 mg.L−1) compared to the commercial source (59.95 mg.L−1). The highest activity of lysozyme and alternative complement activity (ACH50) were found in control and GS1 groups. A significant decrease in alkaline phosphatase activity (ALP) was found in C1 and C2 groups compared to other treatments. Protease activity (P) was significantly decreased in the C2 group compared to the control and GS groups. Total immunoglobulin (total Ig) content was the highest in the control. In addition, total Ig in the GS1 group was higher than GS2. The exposure to ZnO-NPs lowered total protein content in all experimental groups when compared to control. Present findings revealed lower induced immunosuppressive effects by green synthesized ZnO-NPs on key parameters of fish skin mucus.",
publisher = "MDPI AG",
journal = "International Journal of Molecular Sciences",
title = "Chemically and Green Synthesized ZnO Nanoparticles Alter Key Immunological Molecules in Common Carp (Cyprinus carpio) Skin Mucus",
number = "6",
volume = "22",
doi = "10.3390/ijms22063270",
pages = "3270"
}

Rashidian, G., Lazado, C. C., Mahboub, H. H., Mohammadi-Aloucheh, R., Prokić, M., Nada, H. S.,& Faggio, C.. (2021). Chemically and Green Synthesized ZnO Nanoparticles Alter Key Immunological Molecules in Common Carp (Cyprinus carpio) Skin Mucus. in International Journal of Molecular Sciences
MDPI AG., 22(6), 3270.
https://doi.org/10.3390/ijms22063270

Rashidian G, Lazado CC, Mahboub HH, Mohammadi-Aloucheh R, Prokić M, Nada HS, Faggio C. Chemically and Green Synthesized ZnO Nanoparticles Alter Key Immunological Molecules in Common Carp (Cyprinus carpio) Skin Mucus. in International Journal of Molecular Sciences. 2021;22(6):3270.
doi:10.3390/ijms22063270 .

Rashidian, Ghasem, Lazado, Carlo C., Mahboub, Heba H., Mohammadi-Aloucheh, Ramin, Prokić, Marko, Nada, Hend S., Faggio, Caterina, "Chemically and Green Synthesized ZnO Nanoparticles Alter Key Immunological Molecules in Common Carp (Cyprinus carpio) Skin Mucus" in International Journal of Molecular Sciences, 22, no. 6 (2021):3270,
https://doi.org/10.3390/ijms22063270 . .

TY  - JOUR
AU  - Rashidian, Ghasem
AU  - Bahrami Gorji, Sara
AU  - Farsani, Mehdi Naderi
AU  - Prokić, Marko
AU  - Faggio, Caterina
PY  - 2020
UR  - https://www.tandfonline.com/doi/full/10.1080/14786419.2018.1538994
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3223
AB  - Medicinal plants play an important role in aquaculture as feed additives. This study aimed to investigate effects of alcoholic extract of acorn on growth performance, body composition, digestive enzymes activity and blood biochemical parameters of rainbow trout (O. mykiss) as a commercially important fish. Five dietary treatments were supplemented: 100, 200, 400 and 600 mg.kg-1 of the extract. Fishes were fed twice per day for 8 weeks, and results showed that acorn extract positively affected all investigated parameters in rainbow trout fishes. Digestive enzymes activity and growth performance were increased, while activity of liver enzymes and cortisol were lowed in comparison to control individuals. Body composition of treated animals was also enhanced. Comparison between treated groups together with integrative biomarker response (IBR) values indicated greatest effects in animals fed with 400 and 600 mg.kg-1 of the extract. Positive effects of the acorn represent promising start point for further studies.
T2  - Natural Product Research
T1  - The oak (Quercus brantii) acorn as a growth promotor for rainbow trout (Oncorhynchus mykiss): growth performance, body composition, liver enzymes activity and blood biochemical parameters.
IS  - 17
VL  - 34
DO  - 10.1080/14786419.2018.1538994
SP  - 2413
EP  - 2423
ER  - 

@article{
author = "Rashidian, Ghasem and Bahrami Gorji, Sara and Farsani, Mehdi Naderi and Prokić, Marko and Faggio, Caterina",
year = "2020",
abstract = "Medicinal plants play an important role in aquaculture as feed additives. This study aimed to investigate effects of alcoholic extract of acorn on growth performance, body composition, digestive enzymes activity and blood biochemical parameters of rainbow trout (O. mykiss) as a commercially important fish. Five dietary treatments were supplemented: 100, 200, 400 and 600 mg.kg-1 of the extract. Fishes were fed twice per day for 8 weeks, and results showed that acorn extract positively affected all investigated parameters in rainbow trout fishes. Digestive enzymes activity and growth performance were increased, while activity of liver enzymes and cortisol were lowed in comparison to control individuals. Body composition of treated animals was also enhanced. Comparison between treated groups together with integrative biomarker response (IBR) values indicated greatest effects in animals fed with 400 and 600 mg.kg-1 of the extract. Positive effects of the acorn represent promising start point for further studies.",
journal = "Natural Product Research",
title = "The oak (Quercus brantii) acorn as a growth promotor for rainbow trout (Oncorhynchus mykiss): growth performance, body composition, liver enzymes activity and blood biochemical parameters.",
number = "17",
volume = "34",
doi = "10.1080/14786419.2018.1538994",
pages = "2413-2423"
}

Rashidian, G., Bahrami Gorji, S., Farsani, M. N., Prokić, M.,& Faggio, C.. (2020). The oak (Quercus brantii) acorn as a growth promotor for rainbow trout (Oncorhynchus mykiss): growth performance, body composition, liver enzymes activity and blood biochemical parameters.. in Natural Product Research, 34(17), 2413-2423.
https://doi.org/10.1080/14786419.2018.1538994

Rashidian G, Bahrami Gorji S, Farsani MN, Prokić M, Faggio C. The oak (Quercus brantii) acorn as a growth promotor for rainbow trout (Oncorhynchus mykiss): growth performance, body composition, liver enzymes activity and blood biochemical parameters.. in Natural Product Research. 2020;34(17):2413-2423.
doi:10.1080/14786419.2018.1538994 .

Rashidian, Ghasem, Bahrami Gorji, Sara, Farsani, Mehdi Naderi, Prokić, Marko, Faggio, Caterina, "The oak (Quercus brantii) acorn as a growth promotor for rainbow trout (Oncorhynchus mykiss): growth performance, body composition, liver enzymes activity and blood biochemical parameters." in Natural Product Research, 34, no. 17 (2020):2413-2423,
https://doi.org/10.1080/14786419.2018.1538994 . .

TY  - JOUR
AU  - Petrović, Tamara
AU  - Vučić, Tijana
AU  - Nikolić, Sonja Z.
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Faggio, Caterina
AU  - Prokić, Marko
PY  - 2020
UR  - https://www.mdpi.com/2076-2615/10/4/603
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3649
AB  - Shelters are important for animal survival. Provision of adequate hiding places allow animals to express their natural sheltering behavior and it can have different positive effects on cortisol levels, physiological processes and mental performance. Although the absence of a refuge activates some stress response, its effect on oxidative stress has not been adequately examined. This study investigated whether the presence/absence of a shelter modifies the oxidative status (the antioxidant system and oxidative damage) and aggressive behavior of crested newt larvae (Triturus macedonicus and its hybrid with T. ivanbureschi). Our results show that individuals reared with shelters had lower values of the tested antioxidant parameters (catalase, glutathione peroxidase, glutathione S-transferase and glutathione), indicating a lower production of reactive species than individuals reared without shelter. The same pattern was observed in both T. macedonicus and its hybrid. Contrary to the activation of some physiological pathways, shelter availability did not significantly affect the rate of intraspecific aggressive behavior. The physiological benefits of shelter use can be manifested as a lower requirement for investment in the energy necessary for the maintenance of the upregulated antioxidant defenses, activation of repair systems and synthesis of endogenous antioxidants. This study highlights the importance of shelter provision, which may be valuable in habitat restoration and animal conservation studies.
T2  - Animals
T1  - The Effect of Shelter on Oxidative Stress and Aggressive Behavior in Crested Newt Larvae (Triturus spp.)
IS  - 4
VL  - 10
DO  - 10.3390/ani10040603
SP  - 603
ER  - 

@article{
author = "Petrović, Tamara and Vučić, Tijana and Nikolić, Sonja Z. and Gavrić, Jelena and Despotović, Svetlana and Gavrilović, Branka and Radovanović, Tijana and Faggio, Caterina and Prokić, Marko",
year = "2020",
abstract = "Shelters are important for animal survival. Provision of adequate hiding places allow animals to express their natural sheltering behavior and it can have different positive effects on cortisol levels, physiological processes and mental performance. Although the absence of a refuge activates some stress response, its effect on oxidative stress has not been adequately examined. This study investigated whether the presence/absence of a shelter modifies the oxidative status (the antioxidant system and oxidative damage) and aggressive behavior of crested newt larvae (Triturus macedonicus and its hybrid with T. ivanbureschi). Our results show that individuals reared with shelters had lower values of the tested antioxidant parameters (catalase, glutathione peroxidase, glutathione S-transferase and glutathione), indicating a lower production of reactive species than individuals reared without shelter. The same pattern was observed in both T. macedonicus and its hybrid. Contrary to the activation of some physiological pathways, shelter availability did not significantly affect the rate of intraspecific aggressive behavior. The physiological benefits of shelter use can be manifested as a lower requirement for investment in the energy necessary for the maintenance of the upregulated antioxidant defenses, activation of repair systems and synthesis of endogenous antioxidants. This study highlights the importance of shelter provision, which may be valuable in habitat restoration and animal conservation studies.",
journal = "Animals",
title = "The Effect of Shelter on Oxidative Stress and Aggressive Behavior in Crested Newt Larvae (Triturus spp.)",
number = "4",
volume = "10",
doi = "10.3390/ani10040603",
pages = "603"
}

Petrović, T., Vučić, T., Nikolić, S. Z., Gavrić, J., Despotović, S., Gavrilović, B., Radovanović, T., Faggio, C.,& Prokić, M.. (2020). The Effect of Shelter on Oxidative Stress and Aggressive Behavior in Crested Newt Larvae (Triturus spp.). in Animals, 10(4), 603.
https://doi.org/10.3390/ani10040603

Petrović T, Vučić T, Nikolić SZ, Gavrić J, Despotović S, Gavrilović B, Radovanović T, Faggio C, Prokić M. The Effect of Shelter on Oxidative Stress and Aggressive Behavior in Crested Newt Larvae (Triturus spp.). in Animals. 2020;10(4):603.
doi:10.3390/ani10040603 .

Petrović, Tamara, Vučić, Tijana, Nikolić, Sonja Z., Gavrić, Jelena, Despotović, Svetlana, Gavrilović, Branka, Radovanović, Tijana, Faggio, Caterina, Prokić, Marko, "The Effect of Shelter on Oxidative Stress and Aggressive Behavior in Crested Newt Larvae (Triturus spp.)" in Animals, 10, no. 4 (2020):603,
https://doi.org/10.3390/ani10040603 . .

TY  - JOUR
AU  - Rashidian, Ghasem
AU  - Rainis, Simona
AU  - Prokić, Marko
AU  - Faggio, Caterina
PY  - 2020
UR  - https://www.tandfonline.com/doi/full/10.1080/14786419.2020.1723091
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3608
AB  - In this trial, the interaction between two different light resource and different levels of two pigments was investigated in order to determine their effects on growth and reproductive performance of the ornamental fish swordtail (Xiphophorus helleri) broodstock. Fish were fed with different levels of 50, 100 and 200 mg · kg-1 of astaxanthin or canthaxanthin, under two different light conditions (natural and artificial). The obtained results were more evident in animals fed with astaxanthin in comparison to canthaxanthin. Furthermore, swordtails reared under artificial light in comparison to the natural light source showed better performance. Overall the greatest effect was for the fish treated with medium dose of astaxanthin (100 mg.kg-1), under the artificial light conditions of rearing. Results indicated the important effects of all three factors (light source, dosage and carotenoids type), with significant interactions between the carotenoid dosage with carotenoid type and light source.
T2  - Natural Product Research
T1  - Effects of different levels of carotenoids and light sources on swordtail fish (Xiphophorus helleri) growth, survival rate and reproductive parameters.
DO  - 10.1080/14786419.2020.1723091
ER  - 

@article{
author = "Rashidian, Ghasem and Rainis, Simona and Prokić, Marko and Faggio, Caterina",
year = "2020",
abstract = "In this trial, the interaction between two different light resource and different levels of two pigments was investigated in order to determine their effects on growth and reproductive performance of the ornamental fish swordtail (Xiphophorus helleri) broodstock. Fish were fed with different levels of 50, 100 and 200 mg · kg-1 of astaxanthin or canthaxanthin, under two different light conditions (natural and artificial). The obtained results were more evident in animals fed with astaxanthin in comparison to canthaxanthin. Furthermore, swordtails reared under artificial light in comparison to the natural light source showed better performance. Overall the greatest effect was for the fish treated with medium dose of astaxanthin (100 mg.kg-1), under the artificial light conditions of rearing. Results indicated the important effects of all three factors (light source, dosage and carotenoids type), with significant interactions between the carotenoid dosage with carotenoid type and light source.",
journal = "Natural Product Research",
title = "Effects of different levels of carotenoids and light sources on swordtail fish (Xiphophorus helleri) growth, survival rate and reproductive parameters.",
doi = "10.1080/14786419.2020.1723091"
}

Rashidian, G., Rainis, S., Prokić, M.,& Faggio, C.. (2020). Effects of different levels of carotenoids and light sources on swordtail fish (Xiphophorus helleri) growth, survival rate and reproductive parameters.. in Natural Product Research.
https://doi.org/10.1080/14786419.2020.1723091

Rashidian G, Rainis S, Prokić M, Faggio C. Effects of different levels of carotenoids and light sources on swordtail fish (Xiphophorus helleri) growth, survival rate and reproductive parameters.. in Natural Product Research. 2020;.
doi:10.1080/14786419.2020.1723091 .

Rashidian, Ghasem, Rainis, Simona, Prokić, Marko, Faggio, Caterina, "Effects of different levels of carotenoids and light sources on swordtail fish (Xiphophorus helleri) growth, survival rate and reproductive parameters." in Natural Product Research (2020),
https://doi.org/10.1080/14786419.2020.1723091 . .

TY  - JOUR
AU  - Rashidian, Ghasem
AU  - Kajbaf, Kimia
AU  - Prokić, Marko
AU  - Faggio, Caterina
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S1050464819311465?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3579
AB  - The dietary effects of a native medicinal plant from Iran, common mallow (Malvae sylvestris), was evaluated on growth performance, innate immune parameters, mucosal immune parameters, and resistance of rainbow trout (Oncorhynchus mykiss) against Yersinia ruckeri. Therefore, 360 fish (initial weight 10.42 ± 0.09 g) were randomly distributed into 12 fiberglass tanks. Experimental diets supplemented with 0 (as control- C), 1% (M1), 3% (M2) and 5% (M3) levels of M. sylvestris flowers extract were fed to the fish based on 3% of body weight for 8 weeks. At the terminal sampling, growth performance, liver and digestive enzymes activities, blood and mucosal immune responses were determined. Results showed that M2 and M3 had greater final weight, weight gain, SGR, survival rate and lower FCR; higher levels of total protein, albumin, globulin, and lower cortisol levels in comparison to control; 5% extract also lowered cholesterol and glucose levels as well as Lactate Dehydrogenase (LDH) activity. We reported higher values of hematocrit, hemoglobin, Mean Corpuscular Hemoglobin (MCH), Mean Corpuscular Volume (MCV), White Blood Cell (WBC), Red Blood Cell (RBC) and lymphocytes for treated groups. Innate immune responses (Alternative complement activity (ACH50) in M2 and M3 group, total Immunoglobulin (Ig) and lysozyme in M3), mucosal immune parameters (ACH50, total Ig for M2 and M3 group and lysozyme in all treated groups) were enhanced. Activities of digestive enzymes (protease in all treated groups, amylase for 3 and 5%, while lipase only for 5%) and lower activity of liver ALT enzyme in individuals treated with highest dose was observed. Overall results indicated that the extract can positively affect growth performance and immune responses of rainbow trout.
PB  - Academic Press
T2  - Fish & Shellfish Immunology
T1  - Extract of common mallow (Malvae sylvestris) enhances growth, immunity, and resistance of rainbow trout (Oncorhynchus mykiss) fingerlings against Yersinia ruckeri infection.
VL  - 96
DO  - 10.1016/j.fsi.2019.12.018
SP  - 254
EP  - 261
ER  - 

@article{
author = "Rashidian, Ghasem and Kajbaf, Kimia and Prokić, Marko and Faggio, Caterina",
year = "2019",
abstract = "The dietary effects of a native medicinal plant from Iran, common mallow (Malvae sylvestris), was evaluated on growth performance, innate immune parameters, mucosal immune parameters, and resistance of rainbow trout (Oncorhynchus mykiss) against Yersinia ruckeri. Therefore, 360 fish (initial weight 10.42 ± 0.09 g) were randomly distributed into 12 fiberglass tanks. Experimental diets supplemented with 0 (as control- C), 1% (M1), 3% (M2) and 5% (M3) levels of M. sylvestris flowers extract were fed to the fish based on 3% of body weight for 8 weeks. At the terminal sampling, growth performance, liver and digestive enzymes activities, blood and mucosal immune responses were determined. Results showed that M2 and M3 had greater final weight, weight gain, SGR, survival rate and lower FCR; higher levels of total protein, albumin, globulin, and lower cortisol levels in comparison to control; 5% extract also lowered cholesterol and glucose levels as well as Lactate Dehydrogenase (LDH) activity. We reported higher values of hematocrit, hemoglobin, Mean Corpuscular Hemoglobin (MCH), Mean Corpuscular Volume (MCV), White Blood Cell (WBC), Red Blood Cell (RBC) and lymphocytes for treated groups. Innate immune responses (Alternative complement activity (ACH50) in M2 and M3 group, total Immunoglobulin (Ig) and lysozyme in M3), mucosal immune parameters (ACH50, total Ig for M2 and M3 group and lysozyme in all treated groups) were enhanced. Activities of digestive enzymes (protease in all treated groups, amylase for 3 and 5%, while lipase only for 5%) and lower activity of liver ALT enzyme in individuals treated with highest dose was observed. Overall results indicated that the extract can positively affect growth performance and immune responses of rainbow trout.",
publisher = "Academic Press",
journal = "Fish & Shellfish Immunology",
title = "Extract of common mallow (Malvae sylvestris) enhances growth, immunity, and resistance of rainbow trout (Oncorhynchus mykiss) fingerlings against Yersinia ruckeri infection.",
volume = "96",
doi = "10.1016/j.fsi.2019.12.018",
pages = "254-261"
}

Rashidian, G., Kajbaf, K., Prokić, M.,& Faggio, C.. (2019). Extract of common mallow (Malvae sylvestris) enhances growth, immunity, and resistance of rainbow trout (Oncorhynchus mykiss) fingerlings against Yersinia ruckeri infection.. in Fish & Shellfish Immunology
Academic Press., 96, 254-261.
https://doi.org/10.1016/j.fsi.2019.12.018

Rashidian G, Kajbaf K, Prokić M, Faggio C. Extract of common mallow (Malvae sylvestris) enhances growth, immunity, and resistance of rainbow trout (Oncorhynchus mykiss) fingerlings against Yersinia ruckeri infection.. in Fish & Shellfish Immunology. 2019;96:254-261.
doi:10.1016/j.fsi.2019.12.018 .

Rashidian, Ghasem, Kajbaf, Kimia, Prokić, Marko, Faggio, Caterina, "Extract of common mallow (Malvae sylvestris) enhances growth, immunity, and resistance of rainbow trout (Oncorhynchus mykiss) fingerlings against Yersinia ruckeri infection." in Fish & Shellfish Immunology, 96 (2019):254-261,
https://doi.org/10.1016/j.fsi.2019.12.018 . .

TY  - JOUR
AU  - Prokić, Marko
AU  - Radovanović, Tijana
AU  - Gavrić, Jelena
AU  - Faggio, Caterina
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0165993618304710?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3222
AB  - Interest about interactions between microplastics and organisms is on the rise. Accessing organisms' responses to these chemically “inert” compounds plays an important role in determining their potential toxicity. Microplastics from the environment tend to accumulate and move through living organisms, inducing a variety of biological effects, such as disturbances in energy metabolism, oxidative balance, antioxidative capacity, DNA, immunological, neurological and histological damage. In the present review, we summarized the adverse effects of different size, concentrations and types of microplastics on animals' antioxidative system, energy metabolism and nervous system. Results showed that microplastics can: induce oxidative damage (increased lipid peroxidation and DNA strand breaks); alter antioxidative system (superoxide dismutase, catalase and glutathione peroxidase were parameters with the highest and significant changes in activities) and metabolism (isocitrate dehydrogenase and lactate dehydrogenase activity); and have neurotoxic effects (inhibition of acetylcholinesterase activity). The effects were depending on size and dose of used microplastics, and/or their interaction with other xenobiotics. We examined also potential strategies and offer research priorities for current and future studies.
T2  - TrAC Trends in Analytical Chemistry
T1  - Ecotoxicological effects of microplastics: Examination of biomarkers, current state and future perspectives
VL  - 111
DO  - 10.1016/J.TRAC.2018.12.001
SP  - 37
EP  - 46
ER  - 

@article{
author = "Prokić, Marko and Radovanović, Tijana and Gavrić, Jelena and Faggio, Caterina",
year = "2019",
abstract = "Interest about interactions between microplastics and organisms is on the rise. Accessing organisms' responses to these chemically “inert” compounds plays an important role in determining their potential toxicity. Microplastics from the environment tend to accumulate and move through living organisms, inducing a variety of biological effects, such as disturbances in energy metabolism, oxidative balance, antioxidative capacity, DNA, immunological, neurological and histological damage. In the present review, we summarized the adverse effects of different size, concentrations and types of microplastics on animals' antioxidative system, energy metabolism and nervous system. Results showed that microplastics can: induce oxidative damage (increased lipid peroxidation and DNA strand breaks); alter antioxidative system (superoxide dismutase, catalase and glutathione peroxidase were parameters with the highest and significant changes in activities) and metabolism (isocitrate dehydrogenase and lactate dehydrogenase activity); and have neurotoxic effects (inhibition of acetylcholinesterase activity). The effects were depending on size and dose of used microplastics, and/or their interaction with other xenobiotics. We examined also potential strategies and offer research priorities for current and future studies.",
journal = "TrAC Trends in Analytical Chemistry",
title = "Ecotoxicological effects of microplastics: Examination of biomarkers, current state and future perspectives",
volume = "111",
doi = "10.1016/J.TRAC.2018.12.001",
pages = "37-46"
}

Prokić, M., Radovanović, T., Gavrić, J.,& Faggio, C.. (2019). Ecotoxicological effects of microplastics: Examination of biomarkers, current state and future perspectives. in TrAC Trends in Analytical Chemistry, 111, 37-46.
https://doi.org/10.1016/J.TRAC.2018.12.001

Prokić M, Radovanović T, Gavrić J, Faggio C. Ecotoxicological effects of microplastics: Examination of biomarkers, current state and future perspectives. in TrAC Trends in Analytical Chemistry. 2019;111:37-46.
doi:10.1016/J.TRAC.2018.12.001 .

Prokić, Marko, Radovanović, Tijana, Gavrić, Jelena, Faggio, Caterina, "Ecotoxicological effects of microplastics: Examination of biomarkers, current state and future perspectives" in TrAC Trends in Analytical Chemistry, 111 (2019):37-46,
https://doi.org/10.1016/J.TRAC.2018.12.001 . .

TY  - JOUR
AU  - Prokić, Marko
AU  - Petrović, Tamara
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Faggio, Caterina
AU  - Saičić, Zorica
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0944200618300242?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3144
AB  - During their complex life cycle, anurans are faced with various physiological and biochemical demands that can result in increased free radical production. The antioxidative system (AOS), assumes a central role in protection from oxidative stress, and increased knowledge of its response would allow us to identify and quantify underlying costs of free radical production. In this study, we compared the patterns and levels of integration of the AOS during two life stages of Bufotes viridis toads from natural populations: young, fully developed, reproductively inactive subadults and reproductively active adults. All examined parameters (SOD, CAT, GSH-Px, GR, GST, GSH and SH groups) were measured in the liver, skin and muscle. The obtained results showed that: (1) subadult individuals possessed a more integrated AOS (a higher index of integration and number of significant correlations), based mostly on the coordinated action of SOD-CAT; (2) adult individuals used the GSH-Px/GSH system; (3) all examined tissues exhibited similar patterns of variation of AOS parameters; (4) significant correlations between the AOS and biometric parameters. Our results indicate that adult and subadult toads differed in the way they processed free radicals, implying that adults may be more susceptible to oxidative damage. The knowledge from this study will contribute to a better understanding of the AOS and how its activity is modulated in natural populations of anurans throughout their life.
T2  - Zoology
T1  - Comparative assessment of the antioxidative defense system in subadult and adult anurans: A lesson from the Bufotes viridis toad
VL  - 130
DO  - 10.1016/J.ZOOL.2018.08.001
SP  - 30
EP  - 37
ER  - 

@article{
author = "Prokić, Marko and Petrović, Tamara and Gavrić, Jelena and Despotović, Svetlana and Gavrilović, Branka and Radovanović, Tijana and Faggio, Caterina and Saičić, Zorica",
year = "2018",
abstract = "During their complex life cycle, anurans are faced with various physiological and biochemical demands that can result in increased free radical production. The antioxidative system (AOS), assumes a central role in protection from oxidative stress, and increased knowledge of its response would allow us to identify and quantify underlying costs of free radical production. In this study, we compared the patterns and levels of integration of the AOS during two life stages of Bufotes viridis toads from natural populations: young, fully developed, reproductively inactive subadults and reproductively active adults. All examined parameters (SOD, CAT, GSH-Px, GR, GST, GSH and SH groups) were measured in the liver, skin and muscle. The obtained results showed that: (1) subadult individuals possessed a more integrated AOS (a higher index of integration and number of significant correlations), based mostly on the coordinated action of SOD-CAT; (2) adult individuals used the GSH-Px/GSH system; (3) all examined tissues exhibited similar patterns of variation of AOS parameters; (4) significant correlations between the AOS and biometric parameters. Our results indicate that adult and subadult toads differed in the way they processed free radicals, implying that adults may be more susceptible to oxidative damage. The knowledge from this study will contribute to a better understanding of the AOS and how its activity is modulated in natural populations of anurans throughout their life.",
journal = "Zoology",
title = "Comparative assessment of the antioxidative defense system in subadult and adult anurans: A lesson from the Bufotes viridis toad",
volume = "130",
doi = "10.1016/J.ZOOL.2018.08.001",
pages = "30-37"
}

Prokić, M., Petrović, T., Gavrić, J., Despotović, S., Gavrilović, B., Radovanović, T., Faggio, C.,& Saičić, Z.. (2018). Comparative assessment of the antioxidative defense system in subadult and adult anurans: A lesson from the Bufotes viridis toad. in Zoology, 130, 30-37.
https://doi.org/10.1016/J.ZOOL.2018.08.001

Prokić M, Petrović T, Gavrić J, Despotović S, Gavrilović B, Radovanović T, Faggio C, Saičić Z. Comparative assessment of the antioxidative defense system in subadult and adult anurans: A lesson from the Bufotes viridis toad. in Zoology. 2018;130:30-37.
doi:10.1016/J.ZOOL.2018.08.001 .

Prokić, Marko, Petrović, Tamara, Gavrić, Jelena, Despotović, Svetlana, Gavrilović, Branka, Radovanović, Tijana, Faggio, Caterina, Saičić, Zorica, "Comparative assessment of the antioxidative defense system in subadult and adult anurans: A lesson from the Bufotes viridis toad" in Zoology, 130 (2018):30-37,
https://doi.org/10.1016/J.ZOOL.2018.08.001 . .