TY  - DATA
AU  - Ćuković, Katarina
AU  - Todorović, Slađana
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - Bogdanović, Milica
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6214
AB  - This dataset is a product of the doctoral dissertation titled "Identification and expression analysis of genes involved in somatic embryogenesis of centaury (Centaurium erythraea Rafn.)" funded by the Ministry of Education, Science and Technological Development, Republic of Serbia (University of Belgrade, Institute for Biological Research "Siniša Stanković"). The dataset includes the transcript sequence of a newly discovered marker for early somatic embryogenesis in centaury, named CeNA1 (ID:TR23240|c0_g1_i1), as well as gene, gene promoter and corresponding CeNA1 protein sequences for seven homologs that have intact ORF.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
T1  - CeNA1: the newly discovered gene marker for early somatic embryogenesis in centaury (Centaurium erythraea Rafn.) – transcript, gene, gene promoter and protein sequences
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6214
ER  - 

@misc{
author = "Ćuković, Katarina and Todorović, Slađana and Dragićević, Milan and Simonović, Ana and Bogdanović, Milica",
year = "2023",
abstract = "This dataset is a product of the doctoral dissertation titled "Identification and expression analysis of genes involved in somatic embryogenesis of centaury (Centaurium erythraea Rafn.)" funded by the Ministry of Education, Science and Technological Development, Republic of Serbia (University of Belgrade, Institute for Biological Research "Siniša Stanković"). The dataset includes the transcript sequence of a newly discovered marker for early somatic embryogenesis in centaury, named CeNA1 (ID:TR23240|c0_g1_i1), as well as gene, gene promoter and corresponding CeNA1 protein sequences for seven homologs that have intact ORF.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
title = "CeNA1: the newly discovered gene marker for early somatic embryogenesis in centaury (Centaurium erythraea Rafn.) – transcript, gene, gene promoter and protein sequences",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6214"
}

Ćuković, K., Todorović, S., Dragićević, M., Simonović, A.,& Bogdanović, M.. (2023). CeNA1: the newly discovered gene marker for early somatic embryogenesis in centaury (Centaurium erythraea Rafn.) – transcript, gene, gene promoter and protein sequences. 
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_6214

Ćuković K, Todorović S, Dragićević M, Simonović A, Bogdanović M. CeNA1: the newly discovered gene marker for early somatic embryogenesis in centaury (Centaurium erythraea Rafn.) – transcript, gene, gene promoter and protein sequences. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_6214 .

Ćuković, Katarina, Todorović, Slađana, Dragićević, Milan, Simonović, Ana, Bogdanović, Milica, "CeNA1: the newly discovered gene marker for early somatic embryogenesis in centaury (Centaurium erythraea Rafn.) – transcript, gene, gene promoter and protein sequences" (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_6214 .

TY  - CONF
AU  - Simonović, Ana
AU  - Filipović, Biljana
AU  - Banjanac, Tijana
AU  - Kojić, Milan
AU  - Ćuković, Katarina
AU  - Subotić, Angelina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6205
AB  - Biljka kičica (Centaurium erythraea) je interesantna ne samo zbog svojih lekovitih svojstava, već i zbog izuzetne razvojne plastičnosti i regenerative sposobnosti u in vitro kulturi. Kako bi identifikovali gene uključene u morfogenetske procese, sekundarni metabolizam i odgovore na stres, pored sekvenciranih transkriptoma je potreban i sekvenciran genom. Za sekvenciranje genoma najmodernijom PacBio tehnologijom, neophodna je veoma čista visokomolekularna DNK, čijih 90% čine lanci > 20 kb. DNK je izolovana iz mladih listova diploidnog varijeteta kičice gajene in vitro, korišćenjem četiri različita protokola: (1) Quick-DNA HMW MagBead Kit (Zymo Research, #D6060); (2) modifikovani CTAB protokol; (3) protokol za izolaciju bakterijske DNK prilagođen biljnim tkivima i (4) izmenjen protokol za izolaciju RNK iz bora. Kvalitet DNK izolata je proveravan spektrofotometrijski Nano Drop aparatom, običnom elektroforezom, pulsnom elektroforezom i Femto Pulsnom analizom. Protokol (1) daje veoma niske prinose, bilo kad se koristi samostalno ili kao vid prečišćavanja DNK izolovane na drugi način. Protokolom (3) se dobija visok prinos DNK nezadovoljavajućeg kvaliteta. Najkvalitetniju DNK u količinama dovoljnim za sekvenciranje su dali protokoli (2) i (4).
AB  - Биљка кичица (Centaurium erythraea) је интересантна не само због својих
лековитих својстава, већ и због изузетне развојне пластичности и регенеративе
способности у in vitro култури. Како би идентификовали гене укључене у
морфогенетске процесе, секундарни метаболизам и одговоре на стрес, поред
секвенцираних транскриптома1 је потребан и секвенциран геном. За секвенцирање
генома најмодернијом PacBio технологијом, неопходна је веома чиста
високомолекуларна ДНК, чијих 90% чине ланци > 20 kb. ДНК је изолована из
младих листова диплоидног варијетета кичице гајене in vitro, коришћењем четири
различита протокола: (1) Quick-DNA HMW MagBead Kit (Zymo Research, #D6060);
(2) модификовани ЦТАБ протокол;2 (3) протокол за изолацију бактеријске ДНК
прилагођен биљним ткивима и (4) измењен протокол за изолацију РНК из бора.3
Квалитет ДНК изолата је провераван спектрофотометријски Nano Drop апаратом,
обичном електрофорезом, пулсном електрофорезом и Фемто Пулсном анализом.
Протокол (1) даје веома ниске приносе, било кад се користи самостално или као
вид пречишћавања ДНК изоловане на други начин. Протоколом (3) се добија висок
принос ДНК незадовољавајућег квалитета. Најквалитетнију ДНК у количинама
довољним за секвенцирање су дали протоколи (2) и (4).
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Usavršavanje protokola za izolaciju DNK visoke molekularne mase iz kičice (Centaurium erythraea)
T1  - Усавршавање протокола за изолацију ДНК високе молекуларне масе из кичице (Centaurium erythraea)
SP  - 295
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6205
ER  - 

@conference{
author = "Simonović, Ana and Filipović, Biljana and Banjanac, Tijana and Kojić, Milan and Ćuković, Katarina and Subotić, Angelina",
year = "2022",
abstract = "Biljka kičica (Centaurium erythraea) je interesantna ne samo zbog svojih lekovitih svojstava, već i zbog izuzetne razvojne plastičnosti i regenerative sposobnosti u in vitro kulturi. Kako bi identifikovali gene uključene u morfogenetske procese, sekundarni metabolizam i odgovore na stres, pored sekvenciranih transkriptoma je potreban i sekvenciran genom. Za sekvenciranje genoma najmodernijom PacBio tehnologijom, neophodna je veoma čista visokomolekularna DNK, čijih 90% čine lanci > 20 kb. DNK je izolovana iz mladih listova diploidnog varijeteta kičice gajene in vitro, korišćenjem četiri različita protokola: (1) Quick-DNA HMW MagBead Kit (Zymo Research, #D6060); (2) modifikovani CTAB protokol; (3) protokol za izolaciju bakterijske DNK prilagođen biljnim tkivima i (4) izmenjen protokol za izolaciju RNK iz bora. Kvalitet DNK izolata je proveravan spektrofotometrijski Nano Drop aparatom, običnom elektroforezom, pulsnom elektroforezom i Femto Pulsnom analizom. Protokol (1) daje veoma niske prinose, bilo kad se koristi samostalno ili kao vid prečišćavanja DNK izolovane na drugi način. Protokolom (3) se dobija visok prinos DNK nezadovoljavajućeg kvaliteta. Najkvalitetniju DNK u količinama dovoljnim za sekvenciranje su dali protokoli (2) i (4)., Биљка кичица (Centaurium erythraea) је интересантна не само због својих
лековитих својстава, већ и због изузетне развојне пластичности и регенеративе
способности у in vitro култури. Како би идентификовали гене укључене у
морфогенетске процесе, секундарни метаболизам и одговоре на стрес, поред
секвенцираних транскриптома1 је потребан и секвенциран геном. За секвенцирање
генома најмодернијом PacBio технологијом, неопходна је веома чиста
високомолекуларна ДНК, чијих 90% чине ланци > 20 kb. ДНК је изолована из
младих листова диплоидног варијетета кичице гајене in vitro, коришћењем четири
различита протокола: (1) Quick-DNA HMW MagBead Kit (Zymo Research, #D6060);
(2) модификовани ЦТАБ протокол;2 (3) протокол за изолацију бактеријске ДНК
прилагођен биљним ткивима и (4) измењен протокол за изолацију РНК из бора.3
Квалитет ДНК изолата је провераван спектрофотометријски Nano Drop апаратом,
обичном електрофорезом, пулсном електрофорезом и Фемто Пулсном анализом.
Протокол (1) даје веома ниске приносе, било кад се користи самостално или као
вид пречишћавања ДНК изоловане на други начин. Протоколом (3) се добија висок
принос ДНК незадовољавајућег квалитета. Најквалитетнију ДНК у количинама
довољним за секвенцирање су дали протоколи (2) и (4).",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Usavršavanje protokola za izolaciju DNK visoke molekularne mase iz kičice (Centaurium erythraea), Усавршавање протокола за изолацију ДНК високе молекуларне масе из кичице (Centaurium erythraea)",
pages = "295",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6205"
}

Simonović, A., Filipović, B., Banjanac, T., Kojić, M., Ćuković, K.,& Subotić, A.. (2022). Usavršavanje protokola za izolaciju DNK visoke molekularne mase iz kičice (Centaurium erythraea). in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 295.
https://hdl.handle.net/21.15107/rcub_ibiss_6205

Simonović A, Filipović B, Banjanac T, Kojić M, Ćuković K, Subotić A. Usavršavanje protokola za izolaciju DNK visoke molekularne mase iz kičice (Centaurium erythraea). in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:295.
https://hdl.handle.net/21.15107/rcub_ibiss_6205 .

Simonović, Ana, Filipović, Biljana, Banjanac, Tijana, Kojić, Milan, Ćuković, Katarina, Subotić, Angelina, "Usavršavanje protokola za izolaciju DNK visoke molekularne mase iz kičice (Centaurium erythraea)" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):295,
https://hdl.handle.net/21.15107/rcub_ibiss_6205 .

TY  - JOUR
AU  - Rudić, Jelena
AU  - Dragićević, Milan
AU  - Momčilović, Ivana
AU  - Simonović, Ana
AU  - Pantelić, Danijel
PY  - 2022
UR  - https://www.mdpi.com/2076-3921/11/3/488
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4875
AB  - Potato (Solanum tuberosum L.) is the most important vegetable crop globally and is very susceptible to high ambient temperatures. Since heat stress causes the accumulation of reactive oxygen species (ROS), investigations regarding major enzymatic components of the antioxidative system are of the essence. Superoxide dismutases (SODs) represent the first line of defense against ROS but detailed in silico analysis and characterization of the potato SOD gene family have not been performed thus far. We have analyzed eight functional SOD genes, three StCuZnSODs, one StMnSOD, and four StFeSODs, annotated in the updated version of potato genome (Spud DB DM v6.1). The StSOD genes and their respective proteins were analyzed in silico to determine the exon-intron organization, splice variants, cis-regulatory promoter elements, conserved domains, signals for subcellular targeting, 3D-structures, and phylogenetic relations. Quantitative PCR analysis revealed higher induction of StCuZnSODs (the major potato SODs) and StFeSOD3 in thermotolerant cultivar Désirée than in thermosensitive Agria and Kennebec during long-term exposure to elevated temperature. StMnSOD was constitutively expressed, while expression of StFeSODs was cultivar-dependent. The effects of salicylic acid (10−5 M) on StSODs expression were minor. Our results provide the basis for further research on StSODs and their regulation in potato, particularly in response to elevated temperatures.
T2  - Antioxidants
T1  - In Silico Study of Superoxide Dismutase Gene Family in Potato and Effects of Elevated Temperature and Salicylic Acid on Gene Expression
IS  - 3
VL  - 11
DO  - 10.3390/antiox11030488
SP  - 488
ER  - 

@article{
author = "Rudić, Jelena and Dragićević, Milan and Momčilović, Ivana and Simonović, Ana and Pantelić, Danijel",
year = "2022",
abstract = "Potato (Solanum tuberosum L.) is the most important vegetable crop globally and is very susceptible to high ambient temperatures. Since heat stress causes the accumulation of reactive oxygen species (ROS), investigations regarding major enzymatic components of the antioxidative system are of the essence. Superoxide dismutases (SODs) represent the first line of defense against ROS but detailed in silico analysis and characterization of the potato SOD gene family have not been performed thus far. We have analyzed eight functional SOD genes, three StCuZnSODs, one StMnSOD, and four StFeSODs, annotated in the updated version of potato genome (Spud DB DM v6.1). The StSOD genes and their respective proteins were analyzed in silico to determine the exon-intron organization, splice variants, cis-regulatory promoter elements, conserved domains, signals for subcellular targeting, 3D-structures, and phylogenetic relations. Quantitative PCR analysis revealed higher induction of StCuZnSODs (the major potato SODs) and StFeSOD3 in thermotolerant cultivar Désirée than in thermosensitive Agria and Kennebec during long-term exposure to elevated temperature. StMnSOD was constitutively expressed, while expression of StFeSODs was cultivar-dependent. The effects of salicylic acid (10−5 M) on StSODs expression were minor. Our results provide the basis for further research on StSODs and their regulation in potato, particularly in response to elevated temperatures.",
journal = "Antioxidants",
title = "In Silico Study of Superoxide Dismutase Gene Family in Potato and Effects of Elevated Temperature and Salicylic Acid on Gene Expression",
number = "3",
volume = "11",
doi = "10.3390/antiox11030488",
pages = "488"
}

Rudić, J., Dragićević, M., Momčilović, I., Simonović, A.,& Pantelić, D.. (2022). In Silico Study of Superoxide Dismutase Gene Family in Potato and Effects of Elevated Temperature and Salicylic Acid on Gene Expression. in Antioxidants, 11(3), 488.
https://doi.org/10.3390/antiox11030488

Rudić J, Dragićević M, Momčilović I, Simonović A, Pantelić D. In Silico Study of Superoxide Dismutase Gene Family in Potato and Effects of Elevated Temperature and Salicylic Acid on Gene Expression. in Antioxidants. 2022;11(3):488.
doi:10.3390/antiox11030488 .

Rudić, Jelena, Dragićević, Milan, Momčilović, Ivana, Simonović, Ana, Pantelić, Danijel, "In Silico Study of Superoxide Dismutase Gene Family in Potato and Effects of Elevated Temperature and Salicylic Acid on Gene Expression" in Antioxidants, 11, no. 3 (2022):488,
https://doi.org/10.3390/antiox11030488 . .

TY  - CONF
AU  - Ćuković, Katarina
AU  - Bogdanović, Milica
AU  - Simonović, Ana
AU  - Todorović, Slađana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5894
AB  - Описана је метода за транформацију C. erythraea сојем GV3101 А. tumefaciens, 
тестирана на одсечцима листова и врховима коренова биљака гајених in vitro. Сој 
GV3101 носи бинарни плазмид pXK7S2D који обезбеђује резистенцију на 
канамицин. Након кокултивације експлантати су преношени на серију подлога за 
регенерацију са додатком различитих регулатора растења: 1 mg·l

-1 

бензиламинопурина, 0,1 или 0,4 mg·l

-1 индол-3-сирћетне киселине и 1 mg·l-1 
кинетина. Као селекциони антибиотик коришћен је канамицин у концентрацији од 
50 mg·l

-1 за листове или 25 mg·l-1 за коренове, док су цефотаксим и ванкомицин 
концентрација 250 mg·l

-1, односно 50 mg·l-1 додавани у подлогу ради превенције 
раста бактерија. Контролни експлантати су преношени на подлоге истог састава и 
на подлогу без канамицина. Након 6 недеља на листовима су уочени спонтано 
формирани изданци, док на кореновима није запажена регенерација, чак ни на 
контролним експлантатима. Изданци формирани на листовима одсецани су, 
пребацивани на MS подлогу са 0,2 mg·l

-1 индол-3-бутерне киселине и 150 mg·l-1 
цефотаксима и гајени до потпуног формирања биљке. Регенерисане биљке су 
тестиране на присуство трансгена методом Phire PCR-a. Успешност 
трансформације износила је 17,64%. Имајући у виду да је до сада помоћу A. 
tumefaciens трансформисано само неколико биљних врста из фамилије 
Gentianaceae, описана техника за трансформацију листа нуди перспективу за даљу 
примену, нарочито на врстама рода Centaurium.
AB  - Opisana je metoda za tranformaciju C. erythraea sojem GV3101 A. tumefaciens, testirana na odsečcima listova i vrhovima korenova bilјaka gajenih in vitro. Soj GV3101 nosi binarni plazmid pXK7S2D koji obezbeđuje rezistenciju na kanamicin. Nakon kokultivacije eksplantati su prenošeni na seriju podloga za regeneraciju sa dodatkom različitih regulatora rastenja: 1 mg·l -1 benzilaminopurina, 0,1 ili 0,4 mg·l -1 indol-3-sirćetne kiseline i 1 mg·l-1 kinetina. Kao selekcioni antibiotik korišćen je kanamicin u koncentraciji od 50 mg·l -1 za listove ili 25 mg·l-1 za korenove, dok su cefotaksim i vankomicin koncentracija 250 mg·l -1, odnosno 50 mg·l-1 dodavani u podlogu radi prevencije rasta bakterija. Kontrolni eksplantati su prenošeni na podloge istog sastava i na podlogu bez kanamicina. Nakon 6 nedelјa na listovima su uočeni spontano formirani izdanci, dok na korenovima nije zapažena regeneracija, čak ni na kontrolnim eksplantatima. Izdanci formirani na listovima odsecani su, prebacivani na MS podlogu sa 0,2 mg·l -1 indol-3-buterne kiseline i 150 mg·l-1 cefotaksima i gajeni do potpunog formiranja bilјke. Regenerisane bilјke su testirane na prisustvo transgena metodom Phire PCR-a. Uspešnost transformacije iznosila je 17,64%. Imajući u vidu da je do sada pomoću A. tumefaciens transformisano samo nekoliko bilјnih vrsta iz familije Gentianaceae, opisana tehnika za transformaciju lista nudi perspektivu za dalјu primenu, naročito na vrstama roda Centaurium.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Генетичка трансформација листа и корена кичице (Centaurium erythraea) GV3101 сојем A. tumefaciens
T1  - Genetička transformacija lista i korena kičice (Centaurium erythraea) GV3101 sojem A. tumefaciens
SP  - 58
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5894
ER  - 

@conference{
author = "Ćuković, Katarina and Bogdanović, Milica and Simonović, Ana and Todorović, Slađana",
year = "2022",
abstract = "Описана је метода за транформацију C. erythraea сојем GV3101 А. tumefaciens, 
тестирана на одсечцима листова и врховима коренова биљака гајених in vitro. Сој 
GV3101 носи бинарни плазмид pXK7S2D који обезбеђује резистенцију на 
канамицин. Након кокултивације експлантати су преношени на серију подлога за 
регенерацију са додатком различитих регулатора растења: 1 mg·l

-1 

бензиламинопурина, 0,1 или 0,4 mg·l

-1 индол-3-сирћетне киселине и 1 mg·l-1 
кинетина. Као селекциони антибиотик коришћен је канамицин у концентрацији од 
50 mg·l

-1 за листове или 25 mg·l-1 за коренове, док су цефотаксим и ванкомицин 
концентрација 250 mg·l

-1, односно 50 mg·l-1 додавани у подлогу ради превенције 
раста бактерија. Контролни експлантати су преношени на подлоге истог састава и 
на подлогу без канамицина. Након 6 недеља на листовима су уочени спонтано 
формирани изданци, док на кореновима није запажена регенерација, чак ни на 
контролним експлантатима. Изданци формирани на листовима одсецани су, 
пребацивани на MS подлогу са 0,2 mg·l

-1 индол-3-бутерне киселине и 150 mg·l-1 
цефотаксима и гајени до потпуног формирања биљке. Регенерисане биљке су 
тестиране на присуство трансгена методом Phire PCR-a. Успешност 
трансформације износила је 17,64%. Имајући у виду да је до сада помоћу A. 
tumefaciens трансформисано само неколико биљних врста из фамилије 
Gentianaceae, описана техника за трансформацију листа нуди перспективу за даљу 
примену, нарочито на врстама рода Centaurium., Opisana je metoda za tranformaciju C. erythraea sojem GV3101 A. tumefaciens, testirana na odsečcima listova i vrhovima korenova bilјaka gajenih in vitro. Soj GV3101 nosi binarni plazmid pXK7S2D koji obezbeđuje rezistenciju na kanamicin. Nakon kokultivacije eksplantati su prenošeni na seriju podloga za regeneraciju sa dodatkom različitih regulatora rastenja: 1 mg·l -1 benzilaminopurina, 0,1 ili 0,4 mg·l -1 indol-3-sirćetne kiseline i 1 mg·l-1 kinetina. Kao selekcioni antibiotik korišćen je kanamicin u koncentraciji od 50 mg·l -1 za listove ili 25 mg·l-1 za korenove, dok su cefotaksim i vankomicin koncentracija 250 mg·l -1, odnosno 50 mg·l-1 dodavani u podlogu radi prevencije rasta bakterija. Kontrolni eksplantati su prenošeni na podloge istog sastava i na podlogu bez kanamicina. Nakon 6 nedelјa na listovima su uočeni spontano formirani izdanci, dok na korenovima nije zapažena regeneracija, čak ni na kontrolnim eksplantatima. Izdanci formirani na listovima odsecani su, prebacivani na MS podlogu sa 0,2 mg·l -1 indol-3-buterne kiseline i 150 mg·l-1 cefotaksima i gajeni do potpunog formiranja bilјke. Regenerisane bilјke su testirane na prisustvo transgena metodom Phire PCR-a. Uspešnost transformacije iznosila je 17,64%. Imajući u vidu da je do sada pomoću A. tumefaciens transformisano samo nekoliko bilјnih vrsta iz familije Gentianaceae, opisana tehnika za transformaciju lista nudi perspektivu za dalјu primenu, naročito na vrstama roda Centaurium.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Генетичка трансформација листа и корена кичице (Centaurium erythraea) GV3101 сојем A. tumefaciens, Genetička transformacija lista i korena kičice (Centaurium erythraea) GV3101 sojem A. tumefaciens",
pages = "58",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5894"
}

Ćuković, K., Bogdanović, M., Simonović, A.,& Todorović, S.. (2022). Генетичка трансформација листа и корена кичице (Centaurium erythraea) GV3101 сојем A. tumefaciens. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 58.
https://hdl.handle.net/21.15107/rcub_ibiss_5894

Ćuković K, Bogdanović M, Simonović A, Todorović S. Генетичка трансформација листа и корена кичице (Centaurium erythraea) GV3101 сојем A. tumefaciens. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:58.
https://hdl.handle.net/21.15107/rcub_ibiss_5894 .

Ćuković, Katarina, Bogdanović, Milica, Simonović, Ana, Todorović, Slađana, "Генетичка трансформација листа и корена кичице (Centaurium erythraea) GV3101 сојем A. tumefaciens" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):58,
https://hdl.handle.net/21.15107/rcub_ibiss_5894 .

TY  - CONF
AU  - Ćuković, Katarina
AU  - Bogdanović, Milica
AU  - Simonović, Ana
AU  - Todorović, Slađana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5720
AB  - Описана је метода за транформацију C. erythraea сојем GV3101 А. tumefaciens, тестирана на одсечцима листова и врховима коренова биљака гајених in vitro. Сој GV3101 носи бинарни плазмид pXK7S2D који обезбеђује резистенцију на канамицин. Након кокултивације експлантати су преношени на серију подлога за регенерацију са додатком различитих регулатора растења: 1 mg·l-1 бензиламинопурина, 0.1 или 0.4 mg·l-1 индол-3-сирћетне киселине и 1 mg·l-1 кинетина. Као селекциони антибиотик коришћен је канамицин у концентрацији од 50 mg·l-1 за листове или 25 mg·l-1 за коренове, док су цефотаксим и ванкомицин концентрација 250 mg·l-1, односно 50 mg·l-1 додавани у подлогу ради превенције раста бактерија. Контролни експлантати су преношени на подлоге истог састава и на подлогу без канамицина. Након 6 недеља на листовима су уочени спонтано формирани изданци, док на кореновима није запажена регенерација, чак ни на контролним експлантатима. Изданци формирани на листовима одсецани су, пребацивани на MS подлогу са 0.2 mg·l-1 индол-3-бутерне киселине и 150 mg·l-1 цефотаксима и гајени до потпуног формирања биљке. Регенерисане биљке су тестиране на присуство трансгена методом Phire PCR-a.  Успешност трансформације износила је 17.64%. Имајући у виду да је до сада помоћу A. tumefaciens трансформисано само неколико биљних врста из фамилије Gentianaceae, описана техника за трансформацију листа нуди перспективу за даљу примену, нарочито на врстама рода Centaurium.
AB  - Opisana je metoda za tranformaciju C. erythraea sojem GV3101 A. tumefaciens, testirana na odsečcima listova i vrhovima korenova biljaka gajenih in vitro. Soj GV3101 nosi binarni plazmid pXK7S2D koji obezbeđuje rezistenciju na kanamicin. Nakon kokultivacije eksplantati su prenošeni na seriju podloga za regeneraciju sa dodatkom različitih regulatora rastenja: 1 mg·l-1 benzilaminopurina, 0.1 ili 0.4 mg·l-1 indol-3-sirćetne kiseline i 1 mg·l-1 kinetina. Kao selekcioni antibiotik korišćen je kanamicin u koncentraciji od 50 mg·l-1 za listove ili 25 mg·l-1 za korenove, dok su cefotaksim i vankomicin koncentracija 250 mg·l-1, odnosno 50 mg·l-1 dodavani u podlogu radi prevencije rasta bakterija. Kontrolni eksplantati su prenošeni na podloge istog sastava i na podlogu bez kanamicina. Nakon 6 nedelja na listovima su uočeni spontano formirani izdanci, dok na korenovima nije zapažena regeneracija, čak ni na kontrolnim eksplantatima. Izdanci formirani na listovima odsecani su, prebacivani na MS podlogu sa 0.2 mg·l-1 indol-3-buterne kiseline i 150 mg·l-1 cefotaksima i gajeni do potpunog formiranja biljke. Regenerisane biljke su testirane na prisustvo transgena metodom Phire PCR-a. Uspešnost transformacije iznosila je 17.64%. Imajući u vidu da je do sada pomoću A. tumefaciens transformisano samo nekoliko biljnih vrsta iz familije Gentianaceae, opisana tehnika za transformaciju lista nudi perspektivu za dalju primenu, naročito na vrstama roda Centaurium.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - (poster) Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Genetička transformacija lista i korena kičice (Centaurium erythraea) GV3101 sojem A. tumefaciens
T1  - Генетичка трансформација листа и корена кичице (Centaurium erythraea) помоћу GV3101 соја A. tumefaciens
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5720
ER  - 

@conference{
author = "Ćuković, Katarina and Bogdanović, Milica and Simonović, Ana and Todorović, Slađana",
year = "2022",
abstract = "Описана је метода за транформацију C. erythraea сојем GV3101 А. tumefaciens, тестирана на одсечцима листова и врховима коренова биљака гајених in vitro. Сој GV3101 носи бинарни плазмид pXK7S2D који обезбеђује резистенцију на канамицин. Након кокултивације експлантати су преношени на серију подлога за регенерацију са додатком различитих регулатора растења: 1 mg·l-1 бензиламинопурина, 0.1 или 0.4 mg·l-1 индол-3-сирћетне киселине и 1 mg·l-1 кинетина. Као селекциони антибиотик коришћен је канамицин у концентрацији од 50 mg·l-1 за листове или 25 mg·l-1 за коренове, док су цефотаксим и ванкомицин концентрација 250 mg·l-1, односно 50 mg·l-1 додавани у подлогу ради превенције раста бактерија. Контролни експлантати су преношени на подлоге истог састава и на подлогу без канамицина. Након 6 недеља на листовима су уочени спонтано формирани изданци, док на кореновима није запажена регенерација, чак ни на контролним експлантатима. Изданци формирани на листовима одсецани су, пребацивани на MS подлогу са 0.2 mg·l-1 индол-3-бутерне киселине и 150 mg·l-1 цефотаксима и гајени до потпуног формирања биљке. Регенерисане биљке су тестиране на присуство трансгена методом Phire PCR-a.  Успешност трансформације износила је 17.64%. Имајући у виду да је до сада помоћу A. tumefaciens трансформисано само неколико биљних врста из фамилије Gentianaceae, описана техника за трансформацију листа нуди перспективу за даљу примену, нарочито на врстама рода Centaurium., Opisana je metoda za tranformaciju C. erythraea sojem GV3101 A. tumefaciens, testirana na odsečcima listova i vrhovima korenova biljaka gajenih in vitro. Soj GV3101 nosi binarni plazmid pXK7S2D koji obezbeđuje rezistenciju na kanamicin. Nakon kokultivacije eksplantati su prenošeni na seriju podloga za regeneraciju sa dodatkom različitih regulatora rastenja: 1 mg·l-1 benzilaminopurina, 0.1 ili 0.4 mg·l-1 indol-3-sirćetne kiseline i 1 mg·l-1 kinetina. Kao selekcioni antibiotik korišćen je kanamicin u koncentraciji od 50 mg·l-1 za listove ili 25 mg·l-1 za korenove, dok su cefotaksim i vankomicin koncentracija 250 mg·l-1, odnosno 50 mg·l-1 dodavani u podlogu radi prevencije rasta bakterija. Kontrolni eksplantati su prenošeni na podloge istog sastava i na podlogu bez kanamicina. Nakon 6 nedelja na listovima su uočeni spontano formirani izdanci, dok na korenovima nije zapažena regeneracija, čak ni na kontrolnim eksplantatima. Izdanci formirani na listovima odsecani su, prebacivani na MS podlogu sa 0.2 mg·l-1 indol-3-buterne kiseline i 150 mg·l-1 cefotaksima i gajeni do potpunog formiranja biljke. Regenerisane biljke su testirane na prisustvo transgena metodom Phire PCR-a. Uspešnost transformacije iznosila je 17.64%. Imajući u vidu da je do sada pomoću A. tumefaciens transformisano samo nekoliko biljnih vrsta iz familije Gentianaceae, opisana tehnika za transformaciju lista nudi perspektivu za dalju primenu, naročito na vrstama roda Centaurium.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "(poster) Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Genetička transformacija lista i korena kičice (Centaurium erythraea) GV3101 sojem A. tumefaciens, Генетичка трансформација листа и корена кичице (Centaurium erythraea) помоћу GV3101 соја A. tumefaciens",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5720"
}

Ćuković, K., Bogdanović, M., Simonović, A.,& Todorović, S.. (2022). Genetička transformacija lista i korena kičice (Centaurium erythraea) GV3101 sojem A. tumefaciens. in (poster) Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_5720

Ćuković K, Bogdanović M, Simonović A, Todorović S. Genetička transformacija lista i korena kičice (Centaurium erythraea) GV3101 sojem A. tumefaciens. in (poster) Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_5720 .

Ćuković, Katarina, Bogdanović, Milica, Simonović, Ana, Todorović, Slađana, "Genetička transformacija lista i korena kičice (Centaurium erythraea) GV3101 sojem A. tumefaciens" in (poster) Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_5720 .

TY  - CONF
AU  - Ćuković, Katarina
AU  - Bogdanović, Milica
AU  - Simonović, Ana
AU  - Todorović, Slađana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5328
AB  - A genetic transformation method via secondary somatic embryogenesis is described for Centaurium erythraea. Cotyledonary somatic embryos (cse) induced on leaf explants were used for inoculation with A. tumefaciens strain GV3101 containing the binary vector pXK7S2D that provides kanamycin resistance. Inoculated embryos were cultured on MS medium enriched with 2,4-dichlorophenoxyacetic acid and N-(2-chloro-4-pyridyl)-N’-phenylurea that promote somatic embryogenesis, with addition of acetosyringone. Cse were transferred on induction medium supplemented with cefotaxime and 5 or 10 mg/L kanamycin for selection. Embryogenic tissue, induced on primary cse explants during the selection period in the dark, was transferred to hormone-free medium maintaining the same antibiotic combination. Morphologically normal secondary cse that survived kanamycin exposure were isolated and subcultured on hormone-free medium containing cefotaxime and IBA to enhance germination. Fully regenerated plants were analyzed by Phire PCR to determine the transgene presence. Transformation efficiency was higher on media with 10 mg/L kanamycin (17.64%) as compared to 6.67% efficiency obtained on 5 mg/L. To the best of our knowledge, this is the first report on centaury transformation via secondary somatic embryogenesis, which offers an alternative to leaf or root explant transformation and provides an additional tool for investigating in vitro developmental pathways in this plant species.
PB  - Niš : Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Belgrade : Institute for Nature Conservation of Serbia
C3  - Abstracts: 14th Symposium on the flora of southeastern Serbia and neighboring regions; 2022 Jun 26-29; Kladovo, Serbia
T1  - An efficient  Agrobacterium tumefaciens - mediated genetic transformation method for Centaurium erythraea via secondary somatic embrygenesis
SP  - 112
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5328
ER  - 

@conference{
author = "Ćuković, Katarina and Bogdanović, Milica and Simonović, Ana and Todorović, Slađana",
year = "2022",
abstract = "A genetic transformation method via secondary somatic embryogenesis is described for Centaurium erythraea. Cotyledonary somatic embryos (cse) induced on leaf explants were used for inoculation with A. tumefaciens strain GV3101 containing the binary vector pXK7S2D that provides kanamycin resistance. Inoculated embryos were cultured on MS medium enriched with 2,4-dichlorophenoxyacetic acid and N-(2-chloro-4-pyridyl)-N’-phenylurea that promote somatic embryogenesis, with addition of acetosyringone. Cse were transferred on induction medium supplemented with cefotaxime and 5 or 10 mg/L kanamycin for selection. Embryogenic tissue, induced on primary cse explants during the selection period in the dark, was transferred to hormone-free medium maintaining the same antibiotic combination. Morphologically normal secondary cse that survived kanamycin exposure were isolated and subcultured on hormone-free medium containing cefotaxime and IBA to enhance germination. Fully regenerated plants were analyzed by Phire PCR to determine the transgene presence. Transformation efficiency was higher on media with 10 mg/L kanamycin (17.64%) as compared to 6.67% efficiency obtained on 5 mg/L. To the best of our knowledge, this is the first report on centaury transformation via secondary somatic embryogenesis, which offers an alternative to leaf or root explant transformation and provides an additional tool for investigating in vitro developmental pathways in this plant species.",
publisher = "Niš : Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Belgrade : Institute for Nature Conservation of Serbia",
journal = "Abstracts: 14th Symposium on the flora of southeastern Serbia and neighboring regions; 2022 Jun 26-29; Kladovo, Serbia",
title = "An efficient  Agrobacterium tumefaciens - mediated genetic transformation method for Centaurium erythraea via secondary somatic embrygenesis",
pages = "112",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5328"
}

Ćuković, K., Bogdanović, M., Simonović, A.,& Todorović, S.. (2022). An efficient  Agrobacterium tumefaciens - mediated genetic transformation method for Centaurium erythraea via secondary somatic embrygenesis. in Abstracts: 14th Symposium on the flora of southeastern Serbia and neighboring regions; 2022 Jun 26-29; Kladovo, Serbia
Niš : Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 112.
https://hdl.handle.net/21.15107/rcub_ibiss_5328

Ćuković K, Bogdanović M, Simonović A, Todorović S. An efficient  Agrobacterium tumefaciens - mediated genetic transformation method for Centaurium erythraea via secondary somatic embrygenesis. in Abstracts: 14th Symposium on the flora of southeastern Serbia and neighboring regions; 2022 Jun 26-29; Kladovo, Serbia. 2022;:112.
https://hdl.handle.net/21.15107/rcub_ibiss_5328 .

Ćuković, Katarina, Bogdanović, Milica, Simonović, Ana, Todorović, Slađana, "An efficient  Agrobacterium tumefaciens - mediated genetic transformation method for Centaurium erythraea via secondary somatic embrygenesis" in Abstracts: 14th Symposium on the flora of southeastern Serbia and neighboring regions; 2022 Jun 26-29; Kladovo, Serbia (2022):112,
https://hdl.handle.net/21.15107/rcub_ibiss_5328 .

TY  - JOUR
AU  - Filipović, Biljana
AU  - Trifunović-Momčilov, Milana
AU  - Simonović, Ana
AU  - Jevremović, Slađana
AU  - Milošević, Snežana
AU  - Subotić, Angelina
PY  - 2021
UR  - https://link.springer.com/article/10.1007/s11627-020-10143-3
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4231
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4473
AB  - Common centaury (Centaurium erythraea Rafn) is a medicinal plant of great importance for both pharmaceutical and food industries. Distribution of arabinogalactan proteins (AGPs) with specific epitopes recognized by seven monoclonal antibodies (mAbs) was investigated during indirect somatic embryogenesis (ISE) and shoot organogenesis (ISO) in centaury leaf culture. Dynamic changes were observed in localization of JIM4, JIM8, JIM13, JIM15, LM2, LM14, and MAC207 epitopes during somatic embryo (SE) and adventitious bud (AB) development. AGPs responded to all tested mAbs and expressed in numerous meristematic centers that were formed on leaf explants. In globular SEs, the distribution pattern of JIM4, JIM13, JIM15, LM2, and MAC207 epitopes was observed, while during the progression of somatic embryo development, the number of detected AGPs decreased. During formation of ABs, the number of detected AGPs also decreased. In fully formed ABs, only JIM4 and MAC207 were detected. The present study suggests JIM13 antibody as a marker for ISE in centaury leaf culture. These results implicated that AGPs were developmentally regulated during centaury ISE and ISO.
PB  - Springer
T2  - In Vitro Cellular and Developmental Biology - Plant
T1  - Immunolocalization of some arabinogalactan protein epitopes during indirect somatic embryogenesis and shoot organogenesis in leaf culture of centaury (Centaurium erythraea Rafn)
IS  - 3
VL  - 57
DO  - 10.1007/s11627-020-10143-3
SP  - 470
EP  - 480
ER  - 

@article{
author = "Filipović, Biljana and Trifunović-Momčilov, Milana and Simonović, Ana and Jevremović, Slađana and Milošević, Snežana and Subotić, Angelina",
year = "2021",
abstract = "Common centaury (Centaurium erythraea Rafn) is a medicinal plant of great importance for both pharmaceutical and food industries. Distribution of arabinogalactan proteins (AGPs) with specific epitopes recognized by seven monoclonal antibodies (mAbs) was investigated during indirect somatic embryogenesis (ISE) and shoot organogenesis (ISO) in centaury leaf culture. Dynamic changes were observed in localization of JIM4, JIM8, JIM13, JIM15, LM2, LM14, and MAC207 epitopes during somatic embryo (SE) and adventitious bud (AB) development. AGPs responded to all tested mAbs and expressed in numerous meristematic centers that were formed on leaf explants. In globular SEs, the distribution pattern of JIM4, JIM13, JIM15, LM2, and MAC207 epitopes was observed, while during the progression of somatic embryo development, the number of detected AGPs decreased. During formation of ABs, the number of detected AGPs also decreased. In fully formed ABs, only JIM4 and MAC207 were detected. The present study suggests JIM13 antibody as a marker for ISE in centaury leaf culture. These results implicated that AGPs were developmentally regulated during centaury ISE and ISO.",
publisher = "Springer",
journal = "In Vitro Cellular and Developmental Biology - Plant",
title = "Immunolocalization of some arabinogalactan protein epitopes during indirect somatic embryogenesis and shoot organogenesis in leaf culture of centaury (Centaurium erythraea Rafn)",
number = "3",
volume = "57",
doi = "10.1007/s11627-020-10143-3",
pages = "470-480"
}

Filipović, B., Trifunović-Momčilov, M., Simonović, A., Jevremović, S., Milošević, S.,& Subotić, A.. (2021). Immunolocalization of some arabinogalactan protein epitopes during indirect somatic embryogenesis and shoot organogenesis in leaf culture of centaury (Centaurium erythraea Rafn). in In Vitro Cellular and Developmental Biology - Plant
Springer., 57(3), 470-480.
https://doi.org/10.1007/s11627-020-10143-3

Filipović B, Trifunović-Momčilov M, Simonović A, Jevremović S, Milošević S, Subotić A. Immunolocalization of some arabinogalactan protein epitopes during indirect somatic embryogenesis and shoot organogenesis in leaf culture of centaury (Centaurium erythraea Rafn). in In Vitro Cellular and Developmental Biology - Plant. 2021;57(3):470-480.
doi:10.1007/s11627-020-10143-3 .

Filipović, Biljana, Trifunović-Momčilov, Milana, Simonović, Ana, Jevremović, Slađana, Milošević, Snežana, Subotić, Angelina, "Immunolocalization of some arabinogalactan protein epitopes during indirect somatic embryogenesis and shoot organogenesis in leaf culture of centaury (Centaurium erythraea Rafn)" in In Vitro Cellular and Developmental Biology - Plant, 57, no. 3 (2021):470-480,
https://doi.org/10.1007/s11627-020-10143-3 . .

TY  - JOUR
AU  - Đurić, Marija
AU  - Subotić, Angelina
AU  - Prokić, Ljiljana
AU  - Trifunović-Momčilov, Milana
AU  - Cingel, Aleksandar
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - Milošević, Snežana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4119
AB  - Aquaporins comprise a large group of transmembrane proteins responsible for water
transport, which is crucial for plant survival under stress conditions. Despite the vital role of aquaporins,
nothing is known about this protein family in Impatiens walleriana, a commercially important
horticultural plant, which is sensitive to drought stress. In the present study, attention is given to
the molecular characterization of aquaporins in I. walleriana and their expression during drought
stress and recovery. We identified four I. walleriana aquaporins: IwPIP1;4, IwPIP2;2, IwPIP2;7 and
IwTIP4;1. All of them had conserved NPA motifs (Asparagine-Proline-Alanine), transmembrane
helices (TMh), pore characteristics, stereochemical properties and tetrameric structure of holoprotein.
Drought stress and recovery treatment affected the aquaporins expression in I. walleriana
leaves, which was up- or downregulated depending on stress intensity. Expression of IwPIP2;7 was
the most affected of all analyzed I. walleriana aquaporins. At 15% and 5% soil moisture and recovery
from 15% and 5% soil moisture, IwPIP2;7 expression significantly decreased and increased, respectively.
Aquaporins IwPIP1;4 and IwTIP4;1 had lower expression in comparison to IwPIP2;7, with
moderate expression changes in response to drought and recovery, while IwPIP2;2 expression was
of significance only in recovered plants. Insight into the molecular structure of I. walleriana aquaporins
expanded knowledge about plant aquaporins, while its expression during drought and recovery
contributed to I. walleriana drought tolerance mechanisms and re-acclimation.
PB  - Basel, Switzerland: MDPI
T2  - Plants
T1  - Molecular characterization and expression of four aquaporin genes in Impatiens walleriana during drought stress and recovery
IS  - 1
VL  - 10
DO  - 10.3390/plants10010154
SP  - 154
ER  - 

@article{
author = "Đurić, Marija and Subotić, Angelina and Prokić, Ljiljana and Trifunović-Momčilov, Milana and Cingel, Aleksandar and Dragićević, Milan and Simonović, Ana and Milošević, Snežana",
year = "2021",
abstract = "Aquaporins comprise a large group of transmembrane proteins responsible for water
transport, which is crucial for plant survival under stress conditions. Despite the vital role of aquaporins,
nothing is known about this protein family in Impatiens walleriana, a commercially important
horticultural plant, which is sensitive to drought stress. In the present study, attention is given to
the molecular characterization of aquaporins in I. walleriana and their expression during drought
stress and recovery. We identified four I. walleriana aquaporins: IwPIP1;4, IwPIP2;2, IwPIP2;7 and
IwTIP4;1. All of them had conserved NPA motifs (Asparagine-Proline-Alanine), transmembrane
helices (TMh), pore characteristics, stereochemical properties and tetrameric structure of holoprotein.
Drought stress and recovery treatment affected the aquaporins expression in I. walleriana
leaves, which was up- or downregulated depending on stress intensity. Expression of IwPIP2;7 was
the most affected of all analyzed I. walleriana aquaporins. At 15% and 5% soil moisture and recovery
from 15% and 5% soil moisture, IwPIP2;7 expression significantly decreased and increased, respectively.
Aquaporins IwPIP1;4 and IwTIP4;1 had lower expression in comparison to IwPIP2;7, with
moderate expression changes in response to drought and recovery, while IwPIP2;2 expression was
of significance only in recovered plants. Insight into the molecular structure of I. walleriana aquaporins
expanded knowledge about plant aquaporins, while its expression during drought and recovery
contributed to I. walleriana drought tolerance mechanisms and re-acclimation.",
publisher = "Basel, Switzerland: MDPI",
journal = "Plants",
title = "Molecular characterization and expression of four aquaporin genes in Impatiens walleriana during drought stress and recovery",
number = "1",
volume = "10",
doi = "10.3390/plants10010154",
pages = "154"
}

Đurić, M., Subotić, A., Prokić, L., Trifunović-Momčilov, M., Cingel, A., Dragićević, M., Simonović, A.,& Milošević, S.. (2021). Molecular characterization and expression of four aquaporin genes in Impatiens walleriana during drought stress and recovery. in Plants
Basel, Switzerland: MDPI., 10(1), 154.
https://doi.org/10.3390/plants10010154

Đurić M, Subotić A, Prokić L, Trifunović-Momčilov M, Cingel A, Dragićević M, Simonović A, Milošević S. Molecular characterization and expression of four aquaporin genes in Impatiens walleriana during drought stress and recovery. in Plants. 2021;10(1):154.
doi:10.3390/plants10010154 .

Đurić, Marija, Subotić, Angelina, Prokić, Ljiljana, Trifunović-Momčilov, Milana, Cingel, Aleksandar, Dragićević, Milan, Simonović, Ana, Milošević, Snežana, "Molecular characterization and expression of four aquaporin genes in Impatiens walleriana during drought stress and recovery" in Plants, 10, no. 1 (2021):154,
https://doi.org/10.3390/plants10010154 . .

TY  - JOUR
AU  - Bogdanović, Milica
AU  - Ćuković, Katarina
AU  - Subotić, Angelina
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - Filipović, Biljana
AU  - Todorović, Slađana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4126
AB  - Somatic embryogenesis (SE) is a developmental process during which plant somatic cells, under suitable conditions, produce embryogenic cells that develop into somatic embryos (se). SE is the most important method for plant propagation in vitro, having both fundamental and applicative significance. SE can be induced from different tissues and organs, but when se are used as explants, the process is recognized as secondary or cyclic SE. We induced secondary SE in Centaurium erythraea by application of 2,4-dichlorophenoxyacetic acid (2,4-D) and N-(2-chloro-4-pyridyl)-N′-phenylurea (CPPU). A medium containing 0.1 mgL−1 2,4-D and 0.25 mgL−1 CPPU was optimal in terms of the number of primary SE explants forming se, the number of well-developed se per explant, and morphological appearance of the obtained se. These concentrations allowed SE to progress through three cycles, whereas at higher concentrations of 0.2 mgL−1 2,4-D and 0.5 mgL−1 CPPU, only two cycles were achieved. Histological analysis revealed that secondary se are formed both directly and indirectly. Secondary SE readily germinated and converted into plantlets. Induction of cyclic SE contributes to the conservation efforts of this endangered medicinal plant and expands the spectrum of in vitro developmental pathways described in centaury—an emerging model in developmental biology.
PB  - Basel : MDPI
T2  - Plants
T1  - Secondary Somatic Embryogenesis in Centaurium erythraea Rafn
IS  - 2
VL  - 10
DO  - 10.3390/plants10020199
SP  - 199
ER  - 

@article{
author = "Bogdanović, Milica and Ćuković, Katarina and Subotić, Angelina and Dragićević, Milan and Simonović, Ana and Filipović, Biljana and Todorović, Slađana",
year = "2021",
abstract = "Somatic embryogenesis (SE) is a developmental process during which plant somatic cells, under suitable conditions, produce embryogenic cells that develop into somatic embryos (se). SE is the most important method for plant propagation in vitro, having both fundamental and applicative significance. SE can be induced from different tissues and organs, but when se are used as explants, the process is recognized as secondary or cyclic SE. We induced secondary SE in Centaurium erythraea by application of 2,4-dichlorophenoxyacetic acid (2,4-D) and N-(2-chloro-4-pyridyl)-N′-phenylurea (CPPU). A medium containing 0.1 mgL−1 2,4-D and 0.25 mgL−1 CPPU was optimal in terms of the number of primary SE explants forming se, the number of well-developed se per explant, and morphological appearance of the obtained se. These concentrations allowed SE to progress through three cycles, whereas at higher concentrations of 0.2 mgL−1 2,4-D and 0.5 mgL−1 CPPU, only two cycles were achieved. Histological analysis revealed that secondary se are formed both directly and indirectly. Secondary SE readily germinated and converted into plantlets. Induction of cyclic SE contributes to the conservation efforts of this endangered medicinal plant and expands the spectrum of in vitro developmental pathways described in centaury—an emerging model in developmental biology.",
publisher = "Basel : MDPI",
journal = "Plants",
title = "Secondary Somatic Embryogenesis in Centaurium erythraea Rafn",
number = "2",
volume = "10",
doi = "10.3390/plants10020199",
pages = "199"
}

Bogdanović, M., Ćuković, K., Subotić, A., Dragićević, M., Simonović, A., Filipović, B.,& Todorović, S.. (2021). Secondary Somatic Embryogenesis in Centaurium erythraea Rafn. in Plants
Basel : MDPI., 10(2), 199.
https://doi.org/10.3390/plants10020199

Bogdanović M, Ćuković K, Subotić A, Dragićević M, Simonović A, Filipović B, Todorović S. Secondary Somatic Embryogenesis in Centaurium erythraea Rafn. in Plants. 2021;10(2):199.
doi:10.3390/plants10020199 .

Bogdanović, Milica, Ćuković, Katarina, Subotić, Angelina, Dragićević, Milan, Simonović, Ana, Filipović, Biljana, Todorović, Slađana, "Secondary Somatic Embryogenesis in Centaurium erythraea Rafn" in Plants, 10, no. 2 (2021):199,
https://doi.org/10.3390/plants10020199 . .

TY  - JOUR
AU  - Filipović, Biljana
AU  - Trifunović-Momčilov, Milana
AU  - Simonović, Ana
AU  - Jevremović, Slađana
AU  - Milošević, Snežana
AU  - Subotić, Angelina
PY  - 2021
UR  - https://link.springer.com/article/10.1007/s11627-020-10143-3
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4231
AB  - Common centaury (Centaurium erythraea Rafn) is a medicinal plant of great importance for both pharmaceutical and food industries. Distribution of arabinogalactan proteins (AGPs) with specific epitopes recognized by seven monoclonal antibodies (mAbs) was investigated during indirect somatic embryogenesis (ISE) and shoot organogenesis (ISO) in centaury leaf culture. Dynamic changes were observed in localization of JIM4, JIM8, JIM13, JIM15, LM2, LM14, and MAC207 epitopes during somatic embryo (SE) and adventitious bud (AB) development. AGPs responded to all tested mAbs and expressed in numerous meristematic centers that were formed on leaf explants. In globular SEs, the distribution pattern of JIM4, JIM13, JIM15, LM2, and MAC207 epitopes was observed, while during the progression of somatic embryo development, the number of detected AGPs decreased. During formation of ABs, the number of detected AGPs also decreased. In fully formed ABs, only JIM4 and MAC207 were detected. The present study suggests JIM13 antibody as a marker for ISE in centaury leaf culture. These results implicated that AGPs were developmentally regulated during centaury ISE and ISO.
PB  - Springer
T2  - In Vitro Cellular and Developmental Biology - Plant
T1  - Immunolocalization of some arabinogalactan protein epitopes during indirect somatic embryogenesis and shoot organogenesis in leaf culture of centaury (Centaurium erythraea Rafn)
IS  - 3
VL  - 57
DO  - 10.1007/s11627-020-10143-3
SP  - 470
EP  - 480
ER  - 

@article{
author = "Filipović, Biljana and Trifunović-Momčilov, Milana and Simonović, Ana and Jevremović, Slađana and Milošević, Snežana and Subotić, Angelina",
year = "2021",
abstract = "Common centaury (Centaurium erythraea Rafn) is a medicinal plant of great importance for both pharmaceutical and food industries. Distribution of arabinogalactan proteins (AGPs) with specific epitopes recognized by seven monoclonal antibodies (mAbs) was investigated during indirect somatic embryogenesis (ISE) and shoot organogenesis (ISO) in centaury leaf culture. Dynamic changes were observed in localization of JIM4, JIM8, JIM13, JIM15, LM2, LM14, and MAC207 epitopes during somatic embryo (SE) and adventitious bud (AB) development. AGPs responded to all tested mAbs and expressed in numerous meristematic centers that were formed on leaf explants. In globular SEs, the distribution pattern of JIM4, JIM13, JIM15, LM2, and MAC207 epitopes was observed, while during the progression of somatic embryo development, the number of detected AGPs decreased. During formation of ABs, the number of detected AGPs also decreased. In fully formed ABs, only JIM4 and MAC207 were detected. The present study suggests JIM13 antibody as a marker for ISE in centaury leaf culture. These results implicated that AGPs were developmentally regulated during centaury ISE and ISO.",
publisher = "Springer",
journal = "In Vitro Cellular and Developmental Biology - Plant",
title = "Immunolocalization of some arabinogalactan protein epitopes during indirect somatic embryogenesis and shoot organogenesis in leaf culture of centaury (Centaurium erythraea Rafn)",
number = "3",
volume = "57",
doi = "10.1007/s11627-020-10143-3",
pages = "470-480"
}

Filipović, B., Trifunović-Momčilov, M., Simonović, A., Jevremović, S., Milošević, S.,& Subotić, A.. (2021). Immunolocalization of some arabinogalactan protein epitopes during indirect somatic embryogenesis and shoot organogenesis in leaf culture of centaury (Centaurium erythraea Rafn). in In Vitro Cellular and Developmental Biology - Plant
Springer., 57(3), 470-480.
https://doi.org/10.1007/s11627-020-10143-3

Filipović B, Trifunović-Momčilov M, Simonović A, Jevremović S, Milošević S, Subotić A. Immunolocalization of some arabinogalactan protein epitopes during indirect somatic embryogenesis and shoot organogenesis in leaf culture of centaury (Centaurium erythraea Rafn). in In Vitro Cellular and Developmental Biology - Plant. 2021;57(3):470-480.
doi:10.1007/s11627-020-10143-3 .

Filipović, Biljana, Trifunović-Momčilov, Milana, Simonović, Ana, Jevremović, Slađana, Milošević, Snežana, Subotić, Angelina, "Immunolocalization of some arabinogalactan protein epitopes during indirect somatic embryogenesis and shoot organogenesis in leaf culture of centaury (Centaurium erythraea Rafn)" in In Vitro Cellular and Developmental Biology - Plant, 57, no. 3 (2021):470-480,
https://doi.org/10.1007/s11627-020-10143-3 . .

TY  - JOUR
AU  - Paunović, Danijela
AU  - Ćuković, Katarina
AU  - Bogdanović, Milica
AU  - Todorović, Slađana
AU  - Trifunović-Momčilov, Milana
AU  - Subotić, Angelina
AU  - Simonović, Ana
AU  - Dragićević, Milan
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4392
AB  - Centaurium erythraea (centaury) is a medicinal plant with exceptional developmental plasticity in vitro and vigorous, often spontaneous, regeneration via shoot organogenesis and somatic embryogenesis, during which arabinogalactan proteins (AGPs) play an important role. AGPs are highly glycosylated proteins belonging to the super family of O-glycosylated plant cell surface hydroxyproline-rich glycoproteins (HRGPs). HRGPs/AGPs are intrinsically disordered and not well conserved, making their homology-based mining ineffective. We have applied a recently developed pipeline for HRGP/AGP mining, ragp, which is based on machine learning prediction of proline hydroxylation, to identify HRGP sequences in centaury transcriptome and to classify them into motif and amino acid bias (MAAB) classes. AGP sequences with low AG glycomotif representation were also identified. Six members of each of the three AGP subclasses, fasciclin-like AGPs, receptor kinase-like AGPs and AG peptides, were selected for phylogenetic and expression analyses. The expression of these 18 genes was recorded over 48 h following leaf mechanical wounding, as well as in 16 tissue samples representing plants from nature, plants cultivated in vitro, and developmental stages during shoot organogenesis and somatic embryogenesis. None of the selected genes were upregulated during both wounding recovery and regeneration. Possible functions of AGPs with the most interesting expression profiles are discussed.
PB  - Basel: MDPI
T2  - Plants
T1  - The Arabinogalactan Protein Family of Centaurium erythraea Rafn
IS  - 9
VL  - 10
DO  - 10.3390/plants10091870
SP  - 1870
ER  - 

@article{
author = "Paunović, Danijela and Ćuković, Katarina and Bogdanović, Milica and Todorović, Slađana and Trifunović-Momčilov, Milana and Subotić, Angelina and Simonović, Ana and Dragićević, Milan",
year = "2021",
abstract = "Centaurium erythraea (centaury) is a medicinal plant with exceptional developmental plasticity in vitro and vigorous, often spontaneous, regeneration via shoot organogenesis and somatic embryogenesis, during which arabinogalactan proteins (AGPs) play an important role. AGPs are highly glycosylated proteins belonging to the super family of O-glycosylated plant cell surface hydroxyproline-rich glycoproteins (HRGPs). HRGPs/AGPs are intrinsically disordered and not well conserved, making their homology-based mining ineffective. We have applied a recently developed pipeline for HRGP/AGP mining, ragp, which is based on machine learning prediction of proline hydroxylation, to identify HRGP sequences in centaury transcriptome and to classify them into motif and amino acid bias (MAAB) classes. AGP sequences with low AG glycomotif representation were also identified. Six members of each of the three AGP subclasses, fasciclin-like AGPs, receptor kinase-like AGPs and AG peptides, were selected for phylogenetic and expression analyses. The expression of these 18 genes was recorded over 48 h following leaf mechanical wounding, as well as in 16 tissue samples representing plants from nature, plants cultivated in vitro, and developmental stages during shoot organogenesis and somatic embryogenesis. None of the selected genes were upregulated during both wounding recovery and regeneration. Possible functions of AGPs with the most interesting expression profiles are discussed.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "The Arabinogalactan Protein Family of Centaurium erythraea Rafn",
number = "9",
volume = "10",
doi = "10.3390/plants10091870",
pages = "1870"
}

Paunović, D., Ćuković, K., Bogdanović, M., Todorović, S., Trifunović-Momčilov, M., Subotić, A., Simonović, A.,& Dragićević, M.. (2021). The Arabinogalactan Protein Family of Centaurium erythraea Rafn. in Plants
Basel: MDPI., 10(9), 1870.
https://doi.org/10.3390/plants10091870

Paunović D, Ćuković K, Bogdanović M, Todorović S, Trifunović-Momčilov M, Subotić A, Simonović A, Dragićević M. The Arabinogalactan Protein Family of Centaurium erythraea Rafn. in Plants. 2021;10(9):1870.
doi:10.3390/plants10091870 .

Paunović, Danijela, Ćuković, Katarina, Bogdanović, Milica, Todorović, Slađana, Trifunović-Momčilov, Milana, Subotić, Angelina, Simonović, Ana, Dragićević, Milan, "The Arabinogalactan Protein Family of Centaurium erythraea Rafn" in Plants, 10, no. 9 (2021):1870,
https://doi.org/10.3390/plants10091870 . .

TY  - JOUR
AU  - Dragićević, Milan
AU  - Simonović, Ana
PY  - 2021
UR  - https://ojs.pmf.uns.ac.rs/index.php/dbe_serbica/article/view/13522
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4423
AB  - Centaurium erythraea (common centaury) is a medicinal plant with extraordinary developmental plasticity
in vitro that is used as a model organism for studying in vitro morphogenesis in our lab. Several experimental lines of evidence have identified arabinogalactan proteins (AGPs) as one of the key players involved in centaury morphogenesis; however, the role of specific genes has yet to be determined. AGPs are ubiquitous plant cell surface glycoproteins associated with various physiological functions. AGP sequences are characterized by the presence of non-continuous hydroxyproline residues, which serve as O-glycosylation anchor sites for branched arabinogalactans. Due to a biased amino acid composition rich in disorder-promoting amino acids, AGP sequences lack a stable structure and consequently have lessened evolutionary constraints. Therefore, homology-based approaches to AGP sequence mining have limited success. We have recently developed a bioinformatics pipeline for AGP sequence mining, ragp, which exploits their key feature – the presence of hydroxyprolines. This pipeline combines estimation
of proline hydroxylation based on local sequence context by a machine learning model with a flexible motif search. After applying this pipeline to the centaury transcriptome, AGP regions were found to associate with a variety of conserved domains. Here we introduce a streamlined way to train models for prediction of Pro hydroxylation, analyze important protein sequence features determining Pro hydroxylation status, present some of the AGP types found in centaury and discuss model limitations and future prospects.
PB  - Novi Sad: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad
T2  - Biologia Serbica
T1  - Arabinogalactan protein mining and diversity - the case of Centaurium erythraea
IS  - 1
VL  - 43
DO  - 10.5281/zenodo.5512429
SP  - 4
EP  - 11
ER  - 

@article{
author = "Dragićević, Milan and Simonović, Ana",
year = "2021",
abstract = "Centaurium erythraea (common centaury) is a medicinal plant with extraordinary developmental plasticity
in vitro that is used as a model organism for studying in vitro morphogenesis in our lab. Several experimental lines of evidence have identified arabinogalactan proteins (AGPs) as one of the key players involved in centaury morphogenesis; however, the role of specific genes has yet to be determined. AGPs are ubiquitous plant cell surface glycoproteins associated with various physiological functions. AGP sequences are characterized by the presence of non-continuous hydroxyproline residues, which serve as O-glycosylation anchor sites for branched arabinogalactans. Due to a biased amino acid composition rich in disorder-promoting amino acids, AGP sequences lack a stable structure and consequently have lessened evolutionary constraints. Therefore, homology-based approaches to AGP sequence mining have limited success. We have recently developed a bioinformatics pipeline for AGP sequence mining, ragp, which exploits their key feature – the presence of hydroxyprolines. This pipeline combines estimation
of proline hydroxylation based on local sequence context by a machine learning model with a flexible motif search. After applying this pipeline to the centaury transcriptome, AGP regions were found to associate with a variety of conserved domains. Here we introduce a streamlined way to train models for prediction of Pro hydroxylation, analyze important protein sequence features determining Pro hydroxylation status, present some of the AGP types found in centaury and discuss model limitations and future prospects.",
publisher = "Novi Sad: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad",
journal = "Biologia Serbica",
title = "Arabinogalactan protein mining and diversity - the case of Centaurium erythraea",
number = "1",
volume = "43",
doi = "10.5281/zenodo.5512429",
pages = "4-11"
}

Dragićević, M.,& Simonović, A.. (2021). Arabinogalactan protein mining and diversity - the case of Centaurium erythraea. in Biologia Serbica
Novi Sad: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad., 43(1), 4-11.
https://doi.org/10.5281/zenodo.5512429

Dragićević M, Simonović A. Arabinogalactan protein mining and diversity - the case of Centaurium erythraea. in Biologia Serbica. 2021;43(1):4-11.
doi:10.5281/zenodo.5512429 .

Dragićević, Milan, Simonović, Ana, "Arabinogalactan protein mining and diversity - the case of Centaurium erythraea" in Biologia Serbica, 43, no. 1 (2021):4-11,
https://doi.org/10.5281/zenodo.5512429 . .

TY  - JOUR
AU  - Trajković, Milena
AU  - Jevremović, Slađana
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - Subotić, Angelina
AU  - Milošević, Snežana
AU  - Cingel, Aleksandar
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4434
AB  - Flower color is an important characteristic that determines the commercial value of ornamental plants. The development of modern biotechnology methods such as genetic engineering
enables the creation of new flower colors that cannot be achieved with classical methods of hybridization or mutational breeding. This is the first report on the successful Agrobacterium-mediated genetic
transformation of Viola cornuta L. The hypocotyl explants of cv. “Lutea Splendens” variety with
yellow flowers were transformed with A. tumefaciens carrying empty pWBVec10a vector (Llccs−) or
pWBVec10a/CaMV 35S::Llccs::TNos vector (Llccs+) for capsanthin/capsorubin synthase gene (Llccs)
from tiger lily (Lilium lancifolium). A comparative study of shoot multiplication, rooting ability during
culture in vitro, as well as phenotypic characteristics of untransformed (control) and transgenic
Llccs− and Llccs+ plants during ex vitro growth and flowering is presented. Successful integration
of Llccs transgene allows the synthesis of red pigment capsanthin in petal cells that gives flowers
different shades of an orange/reddish color. We demonstrate that the ectopic expression of Llccs gene
in ornamental plants, such as V. cornuta “Lutea Splendens” could successfully be used to change
flower color from yellow to different shades of orange.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Alteration of flower color in Viola cornuta cv. ‘Lutea Splendens’ through metabolic engineering of capsanthin/capsorubin synthesis
IS  - 9
VL  - 7
DO  - 10.3390/horticulturae7090324
SP  - 324
ER  - 

@article{
author = "Trajković, Milena and Jevremović, Slađana and Dragićević, Milan and Simonović, Ana and Subotić, Angelina and Milošević, Snežana and Cingel, Aleksandar",
year = "2021",
abstract = "Flower color is an important characteristic that determines the commercial value of ornamental plants. The development of modern biotechnology methods such as genetic engineering
enables the creation of new flower colors that cannot be achieved with classical methods of hybridization or mutational breeding. This is the first report on the successful Agrobacterium-mediated genetic
transformation of Viola cornuta L. The hypocotyl explants of cv. “Lutea Splendens” variety with
yellow flowers were transformed with A. tumefaciens carrying empty pWBVec10a vector (Llccs−) or
pWBVec10a/CaMV 35S::Llccs::TNos vector (Llccs+) for capsanthin/capsorubin synthase gene (Llccs)
from tiger lily (Lilium lancifolium). A comparative study of shoot multiplication, rooting ability during
culture in vitro, as well as phenotypic characteristics of untransformed (control) and transgenic
Llccs− and Llccs+ plants during ex vitro growth and flowering is presented. Successful integration
of Llccs transgene allows the synthesis of red pigment capsanthin in petal cells that gives flowers
different shades of an orange/reddish color. We demonstrate that the ectopic expression of Llccs gene
in ornamental plants, such as V. cornuta “Lutea Splendens” could successfully be used to change
flower color from yellow to different shades of orange.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Alteration of flower color in Viola cornuta cv. ‘Lutea Splendens’ through metabolic engineering of capsanthin/capsorubin synthesis",
number = "9",
volume = "7",
doi = "10.3390/horticulturae7090324",
pages = "324"
}

Trajković, M., Jevremović, S., Dragićević, M., Simonović, A., Subotić, A., Milošević, S.,& Cingel, A.. (2021). Alteration of flower color in Viola cornuta cv. ‘Lutea Splendens’ through metabolic engineering of capsanthin/capsorubin synthesis. in Horticulturae
Basel: MDPI., 7(9), 324.
https://doi.org/10.3390/horticulturae7090324

Trajković M, Jevremović S, Dragićević M, Simonović A, Subotić A, Milošević S, Cingel A. Alteration of flower color in Viola cornuta cv. ‘Lutea Splendens’ through metabolic engineering of capsanthin/capsorubin synthesis. in Horticulturae. 2021;7(9):324.
doi:10.3390/horticulturae7090324 .

Trajković, Milena, Jevremović, Slađana, Dragićević, Milan, Simonović, Ana, Subotić, Angelina, Milošević, Snežana, Cingel, Aleksandar, "Alteration of flower color in Viola cornuta cv. ‘Lutea Splendens’ through metabolic engineering of capsanthin/capsorubin synthesis" in Horticulturae, 7, no. 9 (2021):324,
https://doi.org/10.3390/horticulturae7090324 . .

TY  - CONF
AU  - Pantelić, Danijel
AU  - Rudić, Jelena
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - Momčilović, Ivana
PY  - 2021
UR  - https://www.vibconferences.be/events/plant-science-for-climate-emergency
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4340
AB  - Potato, Solanum tuberosum L., is the world's most important non-grain food crop, which is highly heat-susceptible. Even moderately elevated temperatures have a significant impact on potato growth and tuberization, limiting productivity. High-temperature stress is accompanied by secondary oxidative stress due to elevation of reactive oxygen species (ROS) levels. Superoxide dismutases (SODs) are metalloenzymes that play an important role of primary ROS scavengers. So far, the SOD gene family was not systematically described in potato, and the data regarding the expression of potato SOD genes under elevated temperature are scarce. We performed an in silico study of potato SODs and analyzed the transcriptional expression in response to moderately elevated (26 °C) and high (29 °C) temperature. The potato genome contains seven SOD genes, including three Cu/ZnSODs, one MnSOD and three FeSODs, unevenly distributed on five chromosomes. Phylogenetic analysis revealed that potato SOD protein sequences share the most similarity to S. lycopersicum SODs and can be separated into two groups: Cu/ZnSOD and FeSOD-MnSOD. Cis-elements related to different signals, including high temperature, plant hormones, and light, were found in promoters of potato SOD genes. Gene expression analysis at different temperature treatments revealed heat-responsive SOD genes. Salicylic acid (SA) is a plant growth regulator and signaling molecule involved in plants' defense responses to abiotic stresses. Application of SA promoted expression of most SODs, especially at moderately elevated (26 °C) temperature. The results of our study revealed heat-inducible SOD genes that might be important for potato antioxidant defense under heat stress.
PB  - VIB Conferences Series
C3  - Plant Science for Climate Emergency | VIB Conferences Program and Abstracts; 2021 June 7-8; Gent, Belgum (Virtual edition), Poster 38, 97.
T1  - The SOD gene family in potato and effects of elevated temperature and salicylic acid on gene expression
SP  - 97 / P38
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4340
ER  - 

@conference{
author = "Pantelić, Danijel and Rudić, Jelena and Dragićević, Milan and Simonović, Ana and Momčilović, Ivana",
year = "2021",
abstract = "Potato, Solanum tuberosum L., is the world's most important non-grain food crop, which is highly heat-susceptible. Even moderately elevated temperatures have a significant impact on potato growth and tuberization, limiting productivity. High-temperature stress is accompanied by secondary oxidative stress due to elevation of reactive oxygen species (ROS) levels. Superoxide dismutases (SODs) are metalloenzymes that play an important role of primary ROS scavengers. So far, the SOD gene family was not systematically described in potato, and the data regarding the expression of potato SOD genes under elevated temperature are scarce. We performed an in silico study of potato SODs and analyzed the transcriptional expression in response to moderately elevated (26 °C) and high (29 °C) temperature. The potato genome contains seven SOD genes, including three Cu/ZnSODs, one MnSOD and three FeSODs, unevenly distributed on five chromosomes. Phylogenetic analysis revealed that potato SOD protein sequences share the most similarity to S. lycopersicum SODs and can be separated into two groups: Cu/ZnSOD and FeSOD-MnSOD. Cis-elements related to different signals, including high temperature, plant hormones, and light, were found in promoters of potato SOD genes. Gene expression analysis at different temperature treatments revealed heat-responsive SOD genes. Salicylic acid (SA) is a plant growth regulator and signaling molecule involved in plants' defense responses to abiotic stresses. Application of SA promoted expression of most SODs, especially at moderately elevated (26 °C) temperature. The results of our study revealed heat-inducible SOD genes that might be important for potato antioxidant defense under heat stress.",
publisher = "VIB Conferences Series",
journal = "Plant Science for Climate Emergency | VIB Conferences Program and Abstracts; 2021 June 7-8; Gent, Belgum (Virtual edition), Poster 38, 97.",
title = "The SOD gene family in potato and effects of elevated temperature and salicylic acid on gene expression",
pages = "97 / P38",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4340"
}

Pantelić, D., Rudić, J., Dragićević, M., Simonović, A.,& Momčilović, I.. (2021). The SOD gene family in potato and effects of elevated temperature and salicylic acid on gene expression. in Plant Science for Climate Emergency | VIB Conferences Program and Abstracts; 2021 June 7-8; Gent, Belgum (Virtual edition), Poster 38, 97.
VIB Conferences Series., 97 / P38.
https://hdl.handle.net/21.15107/rcub_ibiss_4340

Pantelić D, Rudić J, Dragićević M, Simonović A, Momčilović I. The SOD gene family in potato and effects of elevated temperature and salicylic acid on gene expression. in Plant Science for Climate Emergency | VIB Conferences Program and Abstracts; 2021 June 7-8; Gent, Belgum (Virtual edition), Poster 38, 97.. 2021;:97 / P38.
https://hdl.handle.net/21.15107/rcub_ibiss_4340 .

Pantelić, Danijel, Rudić, Jelena, Dragićević, Milan, Simonović, Ana, Momčilović, Ivana, "The SOD gene family in potato and effects of elevated temperature and salicylic acid on gene expression" in Plant Science for Climate Emergency | VIB Conferences Program and Abstracts; 2021 June 7-8; Gent, Belgum (Virtual edition), Poster 38, 97. (2021):97 / P38,
https://hdl.handle.net/21.15107/rcub_ibiss_4340 .

TY  - JOUR
AU  - Simonović, Ana
AU  - Trifunović-Momčilov, Milana
AU  - Filipović, Biljana
AU  - Marković, Marija
AU  - Bogdanović, Milica
AU  - Subotić, Angelina
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4088
AB  - Centaurium erythraea (centaury) is a traditionally used medicinal plant, with a spectrum
of secondary metabolites with confirmed healing properties. Centaury is an emerging model in
plant developmental biology due to its vigorous regenerative potential and great developmental
plasticity when cultured in vitro. Hereby, we review nearly two decades of research on somatic
embryogenesis (SE) in centaury. During SE, somatic cells are induced by suitable culture conditions
to express their totipotency, acquire embryogenic characteristics, and eventually give rise to somatic
embryos. When SE is initiated from centaury root explants, the process occurs spontaneously
(on hormone-free medium), directly (without the callusing phase), and the somatic embryos are
of unicellular origin. SE from leaf explants has to be induced by plant growth regulators and is
indirect (preceded by callusing). Histological observations and culture conditions are compared
in these two systems. The changes in antioxidative enzymes were followed during SE from the
leaf explants. Special focus is given to the role of arabinogalactan proteins during SE, which were
analyzed using a variety of approaches. The newest and preliminary results, including centaury
transcriptome, novel potential SE markers, and novel types of arabinogalactan proteins, are discussed
as perspectives of centaury research.
PB  - MDPI, Basel, Switzerland
T2  - Plants
T1  - Somatic embryogenesis in Centaurium erythraea Rafn - current status and perspectives: a review
VL  - 10
DO  - 10.3390/plants10010070
SP  - 70
ER  - 

@article{
author = "Simonović, Ana and Trifunović-Momčilov, Milana and Filipović, Biljana and Marković, Marija and Bogdanović, Milica and Subotić, Angelina",
year = "2021",
abstract = "Centaurium erythraea (centaury) is a traditionally used medicinal plant, with a spectrum
of secondary metabolites with confirmed healing properties. Centaury is an emerging model in
plant developmental biology due to its vigorous regenerative potential and great developmental
plasticity when cultured in vitro. Hereby, we review nearly two decades of research on somatic
embryogenesis (SE) in centaury. During SE, somatic cells are induced by suitable culture conditions
to express their totipotency, acquire embryogenic characteristics, and eventually give rise to somatic
embryos. When SE is initiated from centaury root explants, the process occurs spontaneously
(on hormone-free medium), directly (without the callusing phase), and the somatic embryos are
of unicellular origin. SE from leaf explants has to be induced by plant growth regulators and is
indirect (preceded by callusing). Histological observations and culture conditions are compared
in these two systems. The changes in antioxidative enzymes were followed during SE from the
leaf explants. Special focus is given to the role of arabinogalactan proteins during SE, which were
analyzed using a variety of approaches. The newest and preliminary results, including centaury
transcriptome, novel potential SE markers, and novel types of arabinogalactan proteins, are discussed
as perspectives of centaury research.",
publisher = "MDPI, Basel, Switzerland",
journal = "Plants",
title = "Somatic embryogenesis in Centaurium erythraea Rafn - current status and perspectives: a review",
volume = "10",
doi = "10.3390/plants10010070",
pages = "70"
}

Simonović, A., Trifunović-Momčilov, M., Filipović, B., Marković, M., Bogdanović, M.,& Subotić, A.. (2021). Somatic embryogenesis in Centaurium erythraea Rafn - current status and perspectives: a review. in Plants
MDPI, Basel, Switzerland., 10, 70.
https://doi.org/10.3390/plants10010070

Simonović A, Trifunović-Momčilov M, Filipović B, Marković M, Bogdanović M, Subotić A. Somatic embryogenesis in Centaurium erythraea Rafn - current status and perspectives: a review. in Plants. 2021;10:70.
doi:10.3390/plants10010070 .

Simonović, Ana, Trifunović-Momčilov, Milana, Filipović, Biljana, Marković, Marija, Bogdanović, Milica, Subotić, Angelina, "Somatic embryogenesis in Centaurium erythraea Rafn - current status and perspectives: a review" in Plants, 10 (2021):70,
https://doi.org/10.3390/plants10010070 . .

TY  - JOUR
AU  - Raspor, Martin
AU  - Motyka, Václav
AU  - Ninković, Slavica
AU  - Malbeck, Jiří
AU  - Dobrev, Petre I.
AU  - Zdravković-Korać, Snežana
AU  - Simonović, Ana
AU  - Ćosić, Tatjana
AU  - Cingel, Aleksandar
AU  - Savić, Jelena
AU  - Zahajská, Lenka
AU  - Tadić, Vojin
AU  - Dragićević, Ivana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3605
AB  - Over the last years, cytokinin deficiency has been studied in a variety of plant species, using transgenic expression of cytokinin oxidase/dehydrogenase genes (CKX). In tobacco and Arabidopsis thaliana, overexpression of A. thaliana CKX genes caused changes in plant morphology known as the "cytokinin deficiency syndrome", including stunted shoot growth, decreased apical dominance, and enhanced root growth. For a valid comparison with the previously obtained in vitro grown AtCKX2-transgenic potato lines, we report here on the construction of transgenic lines of the cultivar Désirée constitutively expressing the gene AtCKX1 under control of the CaMV 35S promoter. Three lines expressing the transgene in both shoot and root tissues were used for analysis. Analyses of CKX activity of tissue extracts, cytokinin levels, morphology, and tuberization parameters revealed similarities as well as differences from the AtCKX2-transgenic plants, including lower levels of CKX activity, and more pronounced morphological effects of cytokinin deficiency. One of the three AtCKX1-overexpressing potato lines was able to start forming tubers before 30 days of growth in long day conditions (16 h/8 h photoperiod) as has been previously observed in some AtCKX2 overexpressors. The extension of the experimental period to 120 days of growth in vitro showed that the other two AtCKX1-overexpressing lines showed impaired tuberization in terms of the number of tubers per plant, and tuber size. We conclude that cytokinin deficiency has distinct effects on tuber induction and tuber initiation/growth, possibly mediated by distinct signaling mechanisms.
T2  - Journal of Plant Growth Regulation
T1  - Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization
VL  - 40
DO  - 10.1007/s00344-020-10080-w
SP  - 37
EP  - 47
ER  - 

@article{
author = "Raspor, Martin and Motyka, Václav and Ninković, Slavica and Malbeck, Jiří and Dobrev, Petre I. and Zdravković-Korać, Snežana and Simonović, Ana and Ćosić, Tatjana and Cingel, Aleksandar and Savić, Jelena and Zahajská, Lenka and Tadić, Vojin and Dragićević, Ivana",
year = "2021",
abstract = "Over the last years, cytokinin deficiency has been studied in a variety of plant species, using transgenic expression of cytokinin oxidase/dehydrogenase genes (CKX). In tobacco and Arabidopsis thaliana, overexpression of A. thaliana CKX genes caused changes in plant morphology known as the "cytokinin deficiency syndrome", including stunted shoot growth, decreased apical dominance, and enhanced root growth. For a valid comparison with the previously obtained in vitro grown AtCKX2-transgenic potato lines, we report here on the construction of transgenic lines of the cultivar Désirée constitutively expressing the gene AtCKX1 under control of the CaMV 35S promoter. Three lines expressing the transgene in both shoot and root tissues were used for analysis. Analyses of CKX activity of tissue extracts, cytokinin levels, morphology, and tuberization parameters revealed similarities as well as differences from the AtCKX2-transgenic plants, including lower levels of CKX activity, and more pronounced morphological effects of cytokinin deficiency. One of the three AtCKX1-overexpressing potato lines was able to start forming tubers before 30 days of growth in long day conditions (16 h/8 h photoperiod) as has been previously observed in some AtCKX2 overexpressors. The extension of the experimental period to 120 days of growth in vitro showed that the other two AtCKX1-overexpressing lines showed impaired tuberization in terms of the number of tubers per plant, and tuber size. We conclude that cytokinin deficiency has distinct effects on tuber induction and tuber initiation/growth, possibly mediated by distinct signaling mechanisms.",
journal = "Journal of Plant Growth Regulation",
title = "Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization",
volume = "40",
doi = "10.1007/s00344-020-10080-w",
pages = "37-47"
}

Raspor, M., Motyka, V., Ninković, S., Malbeck, J., Dobrev, P. I., Zdravković-Korać, S., Simonović, A., Ćosić, T., Cingel, A., Savić, J., Zahajská, L., Tadić, V.,& Dragićević, I.. (2021). Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization. in Journal of Plant Growth Regulation, 40, 37-47.
https://doi.org/10.1007/s00344-020-10080-w

Raspor M, Motyka V, Ninković S, Malbeck J, Dobrev PI, Zdravković-Korać S, Simonović A, Ćosić T, Cingel A, Savić J, Zahajská L, Tadić V, Dragićević I. Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization. in Journal of Plant Growth Regulation. 2021;40:37-47.
doi:10.1007/s00344-020-10080-w .

Raspor, Martin, Motyka, Václav, Ninković, Slavica, Malbeck, Jiří, Dobrev, Petre I., Zdravković-Korać, Snežana, Simonović, Ana, Ćosić, Tatjana, Cingel, Aleksandar, Savić, Jelena, Zahajská, Lenka, Tadić, Vojin, Dragićević, Ivana, "Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization" in Journal of Plant Growth Regulation, 40 (2021):37-47,
https://doi.org/10.1007/s00344-020-10080-w . .

TY  - CONF
AU  - Ćuković, Katarina
AU  - Todorović, Slađana
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - Bogdanović, Milica
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4889
AB  - Centaurium erythraea Rafn is medically significant plant with a great potential in treating multiple gastrointestinal tract diseases. Although widely spread, C. erythraea is now listed as endangered species due to extensive exploitation, so there is a need to deepen knowledge of existing and developnew in vitro techniques for its mass propagation. Somatic embryogenesis (SE) is the most effective way for centaury in vitro regeneration. In addition to possessing great multiplication rate, regeneration via SE is also convenient for genetic transformation since somatic embryos offer genetically uniform starting material with less somaclonal variability. Furthermore, the ability of somatic embryos to undergo secondary SE, a process in which new somatic embryos are initiated from somatic embryos, makes them a suitable target tissue for transformation. We have recently established secondary SE in C. erythraea for the first time and our next step is to develop a transformation method using somatic embryos as starting material. Choice of the correct type and optimal concentration of decontamination and selection antibiotics is crucial in order to obtaina high germination rate and normal morphology of somatic embryos as a prerequisite for successful transformation. Therefore, we evaluated antibiotic sensitivity of untransformed somatic embryos, using different concentrations of cefotaxime and kanamycin as decontamination and selection antibiotics, respectively, and appropriate concentrations were determined. These conclusions were furthermore verified by visual observations of secondary somatic embryos number, their morphology as well as germination rate of embryos grown on media containing various antibiotics concentrations.
PB  - East Sarajevo: Faculty of Agriculture
T1  - Evaluation of kanamycin and cefotaxime effects on proliferation, morphology and germination rate of somatic embryos in Centaurium erythraea Rafn
SP  - 215
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4889
ER  - 

@conference{
author = "Ćuković, Katarina and Todorović, Slađana and Dragićević, Milan and Simonović, Ana and Bogdanović, Milica",
year = "2021",
abstract = "Centaurium erythraea Rafn is medically significant plant with a great potential in treating multiple gastrointestinal tract diseases. Although widely spread, C. erythraea is now listed as endangered species due to extensive exploitation, so there is a need to deepen knowledge of existing and developnew in vitro techniques for its mass propagation. Somatic embryogenesis (SE) is the most effective way for centaury in vitro regeneration. In addition to possessing great multiplication rate, regeneration via SE is also convenient for genetic transformation since somatic embryos offer genetically uniform starting material with less somaclonal variability. Furthermore, the ability of somatic embryos to undergo secondary SE, a process in which new somatic embryos are initiated from somatic embryos, makes them a suitable target tissue for transformation. We have recently established secondary SE in C. erythraea for the first time and our next step is to develop a transformation method using somatic embryos as starting material. Choice of the correct type and optimal concentration of decontamination and selection antibiotics is crucial in order to obtaina high germination rate and normal morphology of somatic embryos as a prerequisite for successful transformation. Therefore, we evaluated antibiotic sensitivity of untransformed somatic embryos, using different concentrations of cefotaxime and kanamycin as decontamination and selection antibiotics, respectively, and appropriate concentrations were determined. These conclusions were furthermore verified by visual observations of secondary somatic embryos number, their morphology as well as germination rate of embryos grown on media containing various antibiotics concentrations.",
publisher = "East Sarajevo: Faculty of Agriculture",
title = "Evaluation of kanamycin and cefotaxime effects on proliferation, morphology and germination rate of somatic embryos in Centaurium erythraea Rafn",
pages = "215",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4889"
}

Ćuković, K., Todorović, S., Dragićević, M., Simonović, A.,& Bogdanović, M.. (2021). Evaluation of kanamycin and cefotaxime effects on proliferation, morphology and germination rate of somatic embryos in Centaurium erythraea Rafn. 
East Sarajevo: Faculty of Agriculture., 215.
https://hdl.handle.net/21.15107/rcub_ibiss_4889

Ćuković K, Todorović S, Dragićević M, Simonović A, Bogdanović M. Evaluation of kanamycin and cefotaxime effects on proliferation, morphology and germination rate of somatic embryos in Centaurium erythraea Rafn. 2021;:215.
https://hdl.handle.net/21.15107/rcub_ibiss_4889 .

Ćuković, Katarina, Todorović, Slađana, Dragićević, Milan, Simonović, Ana, Bogdanović, Milica, "Evaluation of kanamycin and cefotaxime effects on proliferation, morphology and germination rate of somatic embryos in Centaurium erythraea Rafn" (2021):215,
https://hdl.handle.net/21.15107/rcub_ibiss_4889 .

TY  - CONF
AU  - Bogdanović, Milica
AU  - Todorović, Slađana
AU  - Ćuković, Katarina
AU  - Dragićević, Milan
AU  - Beekwilder, Jules
AU  - Cankar, Katarina
AU  - Simonović, Ana
PY  - 2020
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5438
AB  - Chicory (Cichorium intybus L.) is a valuable crop grown mostly in northern France, Belgium and the Netherlands, whose roots, leaves and etiolated shoots are used as vegetable, coffee replacement and remedy for several conditions including inflammation. Roots of chicory are an important source of inulin, a prebiotic fiber and a sweetener. Chicory also contains bioactive terpene compounds that protect the plant against herbivores, but are also responsible for medicinal properties of the plant, as they have anti-microbial, anti-cancer and anti-inflammatory activity. Biosynthesis of plant bioactive metabolites can be modified using new plant breeding techniques, especially new gene editing techniques like CRISPR/Cas9 to improve their health-promoting qualities. Chicory was transformed with Agrobacterium tumefaciens carrying CRISPR/Cas9 constructs to knock-out expression of key genes in the sesquiterpene biosynthesis pathway. Regenerated plants were selected on media with kanamycin and further tested by PCR for the presence of transgenes. Obtained plants were also tested for mutations in target genes, by PCR with primers surrounding the mutation site detecting larger indels and by Sanger sequencing detecting smaller indels. Further analysis was performed detecting heteroduplex DNA fragments as a result of either hetero- or homozygous mutation events.
PB  - East Sarajevo: Faculty of Agriculture
C3  - Book of abstracts: XI International Scientific Agriculture Symposium: Agrosym 2020; 2020 Oct 8-9; Jahorina, Republika Srpska, Bosnia and Herzegovina
T1  - Metabolic engeneering in chicory by CRISPR/Cas9 editing
SP  - 152
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5438
ER  - 

@conference{
author = "Bogdanović, Milica and Todorović, Slađana and Ćuković, Katarina and Dragićević, Milan and Beekwilder, Jules and Cankar, Katarina and Simonović, Ana",
year = "2020",
abstract = "Chicory (Cichorium intybus L.) is a valuable crop grown mostly in northern France, Belgium and the Netherlands, whose roots, leaves and etiolated shoots are used as vegetable, coffee replacement and remedy for several conditions including inflammation. Roots of chicory are an important source of inulin, a prebiotic fiber and a sweetener. Chicory also contains bioactive terpene compounds that protect the plant against herbivores, but are also responsible for medicinal properties of the plant, as they have anti-microbial, anti-cancer and anti-inflammatory activity. Biosynthesis of plant bioactive metabolites can be modified using new plant breeding techniques, especially new gene editing techniques like CRISPR/Cas9 to improve their health-promoting qualities. Chicory was transformed with Agrobacterium tumefaciens carrying CRISPR/Cas9 constructs to knock-out expression of key genes in the sesquiterpene biosynthesis pathway. Regenerated plants were selected on media with kanamycin and further tested by PCR for the presence of transgenes. Obtained plants were also tested for mutations in target genes, by PCR with primers surrounding the mutation site detecting larger indels and by Sanger sequencing detecting smaller indels. Further analysis was performed detecting heteroduplex DNA fragments as a result of either hetero- or homozygous mutation events.",
publisher = "East Sarajevo: Faculty of Agriculture",
journal = "Book of abstracts: XI International Scientific Agriculture Symposium: Agrosym 2020; 2020 Oct 8-9; Jahorina, Republika Srpska, Bosnia and Herzegovina",
title = "Metabolic engeneering in chicory by CRISPR/Cas9 editing",
pages = "152",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5438"
}

Bogdanović, M., Todorović, S., Ćuković, K., Dragićević, M., Beekwilder, J., Cankar, K.,& Simonović, A.. (2020). Metabolic engeneering in chicory by CRISPR/Cas9 editing. in Book of abstracts: XI International Scientific Agriculture Symposium: Agrosym 2020; 2020 Oct 8-9; Jahorina, Republika Srpska, Bosnia and Herzegovina
East Sarajevo: Faculty of Agriculture., 152.
https://hdl.handle.net/21.15107/rcub_ibiss_5438

Bogdanović M, Todorović S, Ćuković K, Dragićević M, Beekwilder J, Cankar K, Simonović A. Metabolic engeneering in chicory by CRISPR/Cas9 editing. in Book of abstracts: XI International Scientific Agriculture Symposium: Agrosym 2020; 2020 Oct 8-9; Jahorina, Republika Srpska, Bosnia and Herzegovina. 2020;:152.
https://hdl.handle.net/21.15107/rcub_ibiss_5438 .

Bogdanović, Milica, Todorović, Slađana, Ćuković, Katarina, Dragićević, Milan, Beekwilder, Jules, Cankar, Katarina, Simonović, Ana, "Metabolic engeneering in chicory by CRISPR/Cas9 editing" in Book of abstracts: XI International Scientific Agriculture Symposium: Agrosym 2020; 2020 Oct 8-9; Jahorina, Republika Srpska, Bosnia and Herzegovina (2020):152,
https://hdl.handle.net/21.15107/rcub_ibiss_5438 .

TY  - JOUR
AU  - Dragićević, Milan
AU  - Paunović, Danijela
AU  - Bogdanović, Milica
AU  - Todorović, Slađana
AU  - Simonović, Ana
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3756
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4966
AB  - Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones. While this diversity is responsible for a wide array of physiological functions associated with HRGPs, it hinders attempts for homology-based identification. Current approaches, based on identifying sequences with characteristic motifs and biased amino acid composition, are limited to prototypical sequences. Ragp is an R package for mining and analysis of HRGPs, with emphasis on arabinogalactan proteins. The ragp filtering pipeline exploits one of the HRGPs key features, the presence of hydroxyprolines which represent glycosylation sites. Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains. As such, ragp extends R’s rich ecosystem for high-throughput sequence data analyses. The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.
PB  - Oxford University Press
T2  - Glycobiology
T1  - ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R
IS  - 1
IS  - 1
VL  - 30
VL  - 30
DO  - 10.1093/glycob/cwz072
SP  - 19
SP  - 19
EP  - 35
EP  - 35
ER  - 

@article{
author = "Dragićević, Milan and Paunović, Danijela and Bogdanović, Milica and Todorović, Slađana and Simonović, Ana",
year = "2020",
abstract = "Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones. While this diversity is responsible for a wide array of physiological functions associated with HRGPs, it hinders attempts for homology-based identification. Current approaches, based on identifying sequences with characteristic motifs and biased amino acid composition, are limited to prototypical sequences. Ragp is an R package for mining and analysis of HRGPs, with emphasis on arabinogalactan proteins. The ragp filtering pipeline exploits one of the HRGPs key features, the presence of hydroxyprolines which represent glycosylation sites. Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains. As such, ragp extends R’s rich ecosystem for high-throughput sequence data analyses. The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.",
publisher = "Oxford University Press",
journal = "Glycobiology",
title = "ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R",
number = "1, 1",
volume = "30, 30",
doi = "10.1093/glycob/cwz072",
pages = "19-19-35-35"
}

Dragićević, M., Paunović, D., Bogdanović, M., Todorović, S.,& Simonović, A.. (2020). ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. in Glycobiology
Oxford University Press., 30(1), 19-35.
https://doi.org/10.1093/glycob/cwz072

Dragićević M, Paunović D, Bogdanović M, Todorović S, Simonović A. ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. in Glycobiology. 2020;30(1):19-35.
doi:10.1093/glycob/cwz072 .

Dragićević, Milan, Paunović, Danijela, Bogdanović, Milica, Todorović, Slađana, Simonović, Ana, "ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R" in Glycobiology, 30, no. 1 (2020):19-35,
https://doi.org/10.1093/glycob/cwz072 . .

TY  - JOUR
AU  - Dragićević, Milan
AU  - Paunović, Danijela
AU  - Bogdanović, Milica
AU  - Todorović, Slađana
AU  - Simonović, Ana
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3756
AB  - Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones. While this diversity is responsible for a wide array of physiological functions associated with HRGPs, it hinders attempts for homology-based identification. Current approaches, based on identifying sequences with characteristic motifs and biased amino acid composition, are limited to prototypical sequences. Ragp is an R package for mining and analysis of HRGPs, with emphasis on arabinogalactan proteins. The ragp filtering pipeline exploits one of the HRGPs key features, the presence of hydroxyprolines which represent glycosylation sites. Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains. As such, ragp extends R’s rich ecosystem for high-throughput sequence data analyses. The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.
PB  - Oxford University Press
T2  - Glycobiology
T1  - ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R
IS  - 1
IS  - 1
VL  - 30
VL  - 30
DO  - 10.1093/glycob/cwz072
SP  - 19
SP  - 19
EP  - 35
EP  - 35
ER  - 

@article{
author = "Dragićević, Milan and Paunović, Danijela and Bogdanović, Milica and Todorović, Slađana and Simonović, Ana",
year = "2020",
abstract = "Hydroxyproline-rich glycoproteins (HRGPs) are one of the most complex families of macromolecules found in plants, due to the diversity of glycans decorating the protein backbone, as well as the heterogeneity of the protein backbones. While this diversity is responsible for a wide array of physiological functions associated with HRGPs, it hinders attempts for homology-based identification. Current approaches, based on identifying sequences with characteristic motifs and biased amino acid composition, are limited to prototypical sequences. Ragp is an R package for mining and analysis of HRGPs, with emphasis on arabinogalactan proteins. The ragp filtering pipeline exploits one of the HRGPs key features, the presence of hydroxyprolines which represent glycosylation sites. Main package features include prediction of proline hydroxylation sites, amino acid motif and bias analyses, efficient communication with web servers for prediction of N-terminal signal peptides, glycosylphosphatidylinositol modification sites and disordered regions and the ability to annotate sequences through hmmscan and subsequent GO enrichment, based on predicted Pfam domains. As such, ragp extends R’s rich ecosystem for high-throughput sequence data analyses. The ragp R package is available under the MIT Open Source license and is freely available to download from GitHub at: https://github.com/missuse/ragp.",
publisher = "Oxford University Press",
journal = "Glycobiology",
title = "ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R",
number = "1, 1",
volume = "30, 30",
doi = "10.1093/glycob/cwz072",
pages = "19-19-35-35"
}

Dragićević, M., Paunović, D., Bogdanović, M., Todorović, S.,& Simonović, A.. (2020). ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. in Glycobiology
Oxford University Press., 30(1), 19-35.
https://doi.org/10.1093/glycob/cwz072

Dragićević M, Paunović D, Bogdanović M, Todorović S, Simonović A. ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R. in Glycobiology. 2020;30(1):19-35.
doi:10.1093/glycob/cwz072 .

Dragićević, Milan, Paunović, Danijela, Bogdanović, Milica, Todorović, Slađana, Simonović, Ana, "ragp: Pipeline for mining of plant hydroxyproline-rich glycoproteins with implementation in R" in Glycobiology, 30, no. 1 (2020):19-35,
https://doi.org/10.1093/glycob/cwz072 . .

TY  - JOUR
AU  - Antonić Reljin, Dragana
AU  - Subotić, Angelina
AU  - Dragićević, Milan
AU  - Pantelić, Danijel
AU  - Milošević, Snežana
AU  - Simonović, Ana
AU  - Momčilović, Ivana
PY  - 2020
UR  - https://www.mdpi.com/2223-7747/9/11/1589
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4009
AB  - Impatiens walleriana is a valued ornamental plant sensitive to drought stress. We investigated whether the foliar application of 2mM salicylic acid (SA) can protect potted I. walleriana plants from drought stress. The plants were divided into: watered plants, drought-stressed plants, watered plants treated with SA and drought-stressed plants treated with SA. The number of flowers and flower buds, relative water content (RWC), contents of malondialdehyde (MDA) and proline (Pro) and the activities of superoxide dismutases, catalases and peroxidases were recorded at different time points. Three dehydrin sequences were identified in de novo assembled leaf transcriptome: IwDhn1, IwDhn2.1 and IwDhn2.2. Drought stress caused wilting, floral abortion, reduction of RWC and increased MDA—an indicator of lipid peroxidation. In response to drought, Impatiens accumulated Pro and induced chloroplastic Cu/ZnSOD and two peroxidase isoforms. The most remarkable drought response was strong induction of IwDhn2.1 and IwDhn2.2. Rehydration restored RWC, Pro level, Cu/ZnSOD activity and dehydrins expression in drought-stressed plants approximately to the values of watered plants.SA had ameliorating effects on plants exposed to drought, including prevention of wilting, preservation of RWC, increased Pro accumulation, modulation of antioxidative activities and remarkable decrease of lipid peroxidation, but without effects on flowers’ preservation.
PB  - MDPI AG
T2  - Plants
T1  - Effects of Exogenous Salicylic Acid on Drought Response and Characterization of Dehydrins in Impatiens walleriana
IS  - 11
VL  - 9
DO  - 10.3390/plants9111589
SP  - 1589
ER  - 

@article{
author = "Antonić Reljin, Dragana and Subotić, Angelina and Dragićević, Milan and Pantelić, Danijel and Milošević, Snežana and Simonović, Ana and Momčilović, Ivana",
year = "2020",
abstract = "Impatiens walleriana is a valued ornamental plant sensitive to drought stress. We investigated whether the foliar application of 2mM salicylic acid (SA) can protect potted I. walleriana plants from drought stress. The plants were divided into: watered plants, drought-stressed plants, watered plants treated with SA and drought-stressed plants treated with SA. The number of flowers and flower buds, relative water content (RWC), contents of malondialdehyde (MDA) and proline (Pro) and the activities of superoxide dismutases, catalases and peroxidases were recorded at different time points. Three dehydrin sequences were identified in de novo assembled leaf transcriptome: IwDhn1, IwDhn2.1 and IwDhn2.2. Drought stress caused wilting, floral abortion, reduction of RWC and increased MDA—an indicator of lipid peroxidation. In response to drought, Impatiens accumulated Pro and induced chloroplastic Cu/ZnSOD and two peroxidase isoforms. The most remarkable drought response was strong induction of IwDhn2.1 and IwDhn2.2. Rehydration restored RWC, Pro level, Cu/ZnSOD activity and dehydrins expression in drought-stressed plants approximately to the values of watered plants.SA had ameliorating effects on plants exposed to drought, including prevention of wilting, preservation of RWC, increased Pro accumulation, modulation of antioxidative activities and remarkable decrease of lipid peroxidation, but without effects on flowers’ preservation.",
publisher = "MDPI AG",
journal = "Plants",
title = "Effects of Exogenous Salicylic Acid on Drought Response and Characterization of Dehydrins in Impatiens walleriana",
number = "11",
volume = "9",
doi = "10.3390/plants9111589",
pages = "1589"
}

Antonić Reljin, D., Subotić, A., Dragićević, M., Pantelić, D., Milošević, S., Simonović, A.,& Momčilović, I.. (2020). Effects of Exogenous Salicylic Acid on Drought Response and Characterization of Dehydrins in Impatiens walleriana. in Plants
MDPI AG., 9(11), 1589.
https://doi.org/10.3390/plants9111589

Antonić Reljin D, Subotić A, Dragićević M, Pantelić D, Milošević S, Simonović A, Momčilović I. Effects of Exogenous Salicylic Acid on Drought Response and Characterization of Dehydrins in Impatiens walleriana. in Plants. 2020;9(11):1589.
doi:10.3390/plants9111589 .

Antonić Reljin, Dragana, Subotić, Angelina, Dragićević, Milan, Pantelić, Danijel, Milošević, Snežana, Simonović, Ana, Momčilović, Ivana, "Effects of Exogenous Salicylic Acid on Drought Response and Characterization of Dehydrins in Impatiens walleriana" in Plants, 9, no. 11 (2020):1589,
https://doi.org/10.3390/plants9111589 . .

TY  - JOUR
AU  - Bogdanović, Milica
AU  - Cankar, Katarina
AU  - Dragićević, Milan
AU  - Bouwmeester, Harro
AU  - Beekwilder, Jules
AU  - Simonović, Ana
AU  - Todorović, Slađana
PY  - 2020
UR  - https://www.tandfonline.com/doi/full/10.1080/21645698.2019.1681868
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3520
AB  - Chicory (Cichorium intybus L.) is a medicinal and industrial plant from the Asteraceae family that produces a variety of sesquiterpene lactones (STLs), most importantly bitter guaianolides: lactucin, lactucopicrin and 8-deoxylactucin as well as their modified forms such as oxalates. These compounds have medicinal properties; however, they also hamper the extraction of inulin - a very important food industry product from chicory roots. The first step in guaianolide biosynthesis is catalyzed by germacrene A synthase (GAS) which in chicory exists in two isoforms - GAS long (encoded by CiGASlo) and GAS short (encoded by CiGASsh). AmiRNA silencing was used to obtain plants with reduced GAS gene expression and level of downstream metabolites, guaianolide-15-oxalates, as the major STLs in chicory. This approach could be beneficial for engineering new chicory varieties with varying STL content, and especially varieties with reduced bitter compounds more suitable for inulin production.
T2  - GM Crops & Food
T1  - Silencing of germacrene A synthase genes reduces guaianolide oxalate content in Cichorium intybus L.
IS  - 1
VL  - 11
DO  - 10.1080/21645698.2019.1681868
SP  - 54
EP  - 66
ER  - 

@article{
author = "Bogdanović, Milica and Cankar, Katarina and Dragićević, Milan and Bouwmeester, Harro and Beekwilder, Jules and Simonović, Ana and Todorović, Slađana",
year = "2020",
abstract = "Chicory (Cichorium intybus L.) is a medicinal and industrial plant from the Asteraceae family that produces a variety of sesquiterpene lactones (STLs), most importantly bitter guaianolides: lactucin, lactucopicrin and 8-deoxylactucin as well as their modified forms such as oxalates. These compounds have medicinal properties; however, they also hamper the extraction of inulin - a very important food industry product from chicory roots. The first step in guaianolide biosynthesis is catalyzed by germacrene A synthase (GAS) which in chicory exists in two isoforms - GAS long (encoded by CiGASlo) and GAS short (encoded by CiGASsh). AmiRNA silencing was used to obtain plants with reduced GAS gene expression and level of downstream metabolites, guaianolide-15-oxalates, as the major STLs in chicory. This approach could be beneficial for engineering new chicory varieties with varying STL content, and especially varieties with reduced bitter compounds more suitable for inulin production.",
journal = "GM Crops & Food",
title = "Silencing of germacrene A synthase genes reduces guaianolide oxalate content in Cichorium intybus L.",
number = "1",
volume = "11",
doi = "10.1080/21645698.2019.1681868",
pages = "54-66"
}

Bogdanović, M., Cankar, K., Dragićević, M., Bouwmeester, H., Beekwilder, J., Simonović, A.,& Todorović, S.. (2020). Silencing of germacrene A synthase genes reduces guaianolide oxalate content in Cichorium intybus L.. in GM Crops & Food, 11(1), 54-66.
https://doi.org/10.1080/21645698.2019.1681868

Bogdanović M, Cankar K, Dragićević M, Bouwmeester H, Beekwilder J, Simonović A, Todorović S. Silencing of germacrene A synthase genes reduces guaianolide oxalate content in Cichorium intybus L.. in GM Crops & Food. 2020;11(1):54-66.
doi:10.1080/21645698.2019.1681868 .

Bogdanović, Milica, Cankar, Katarina, Dragićević, Milan, Bouwmeester, Harro, Beekwilder, Jules, Simonović, Ana, Todorović, Slađana, "Silencing of germacrene A synthase genes reduces guaianolide oxalate content in Cichorium intybus L." in GM Crops & Food, 11, no. 1 (2020):54-66,
https://doi.org/10.1080/21645698.2019.1681868 . .

TY  - JOUR
AU  - Ćuković, Katarina
AU  - Dragićević, Milan
AU  - Bogdanović, Milica
AU  - Paunović, Danijela
AU  - Giurato, Giorgio
AU  - Filipović, Biljana
AU  - Subotić, Angelina
AU  - Todorović, Slađana
AU  - Simonović, Ana
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3686
AB  - Centaurium erythraea Rafn. (common centaury, Gentianaceae) is a medicinal plant with great regeneration potential and developmental plasticity in vitro. Centaury can be regenerated from leaf explants by both somatic embryogenesis (SE) and shoot development (SD). We believe that its regeneration potential and developmental plasticity rest on high activity of certain genes, which may not be active or present in species recalcitrant to in vitro regeneration. However, there are no sequenced Gentianaceae genomes to support investigation of the molecular events during SE or SD. To this end, we have sequenced six centaury transcriptomes (embryogenic calli, globular somatic embryos, cotyledonary somatic embryos, adventitious buds, leaves and roots of in vitro grown plants) and de novo assembled centaury referent transcriptome comprising 105.726 genes. The high quality and completeness transcriptome was functionally annotated against NCBI nt, Swissprot and PFAM databases with KOG and GO enrichment. In addition, 11 housekeeping and functional genes were validated for expression stability in 27 tissue samples representing the processes of SE and SD, plants from nature and wounded tissues using GeNorm and NormFinder. The most stable genes that can be used for expression studies during SE, SD and in vitro manipulations are Ribosomal protein L2 (RPL2) and TATA binding protein 1 (TBP1) in combination with RAS (Rat Sarcoma)-related Nuclear protein (RAN) or Adenosine kinase (AK). These results comprise a complete framework for the search for genes involved in SE and SD, but may also be useful in identifying genes involved in biosynthesis of C. erythraea secondary metabolites.
PB  - Springer
T2  - Plant Cell, Tissue and Organ Culture
T1  - Plant regeneration in leaf culture of Centaurium erythraea Rafn. Part 3: de novo transcriptome assembly and validation of housekeeping genes for studies of in vitro morphogenesis
IS  - 2
VL  - 141
DO  - 10.1007/s11240-020-01801-w
SP  - 417
EP  - 433
ER  - 

@article{
author = "Ćuković, Katarina and Dragićević, Milan and Bogdanović, Milica and Paunović, Danijela and Giurato, Giorgio and Filipović, Biljana and Subotić, Angelina and Todorović, Slađana and Simonović, Ana",
year = "2020",
abstract = "Centaurium erythraea Rafn. (common centaury, Gentianaceae) is a medicinal plant with great regeneration potential and developmental plasticity in vitro. Centaury can be regenerated from leaf explants by both somatic embryogenesis (SE) and shoot development (SD). We believe that its regeneration potential and developmental plasticity rest on high activity of certain genes, which may not be active or present in species recalcitrant to in vitro regeneration. However, there are no sequenced Gentianaceae genomes to support investigation of the molecular events during SE or SD. To this end, we have sequenced six centaury transcriptomes (embryogenic calli, globular somatic embryos, cotyledonary somatic embryos, adventitious buds, leaves and roots of in vitro grown plants) and de novo assembled centaury referent transcriptome comprising 105.726 genes. The high quality and completeness transcriptome was functionally annotated against NCBI nt, Swissprot and PFAM databases with KOG and GO enrichment. In addition, 11 housekeeping and functional genes were validated for expression stability in 27 tissue samples representing the processes of SE and SD, plants from nature and wounded tissues using GeNorm and NormFinder. The most stable genes that can be used for expression studies during SE, SD and in vitro manipulations are Ribosomal protein L2 (RPL2) and TATA binding protein 1 (TBP1) in combination with RAS (Rat Sarcoma)-related Nuclear protein (RAN) or Adenosine kinase (AK). These results comprise a complete framework for the search for genes involved in SE and SD, but may also be useful in identifying genes involved in biosynthesis of C. erythraea secondary metabolites.",
publisher = "Springer",
journal = "Plant Cell, Tissue and Organ Culture",
title = "Plant regeneration in leaf culture of Centaurium erythraea Rafn. Part 3: de novo transcriptome assembly and validation of housekeeping genes for studies of in vitro morphogenesis",
number = "2",
volume = "141",
doi = "10.1007/s11240-020-01801-w",
pages = "417-433"
}

Ćuković, K., Dragićević, M., Bogdanović, M., Paunović, D., Giurato, G., Filipović, B., Subotić, A., Todorović, S.,& Simonović, A.. (2020). Plant regeneration in leaf culture of Centaurium erythraea Rafn. Part 3: de novo transcriptome assembly and validation of housekeeping genes for studies of in vitro morphogenesis. in Plant Cell, Tissue and Organ Culture
Springer., 141(2), 417-433.
https://doi.org/10.1007/s11240-020-01801-w

Ćuković K, Dragićević M, Bogdanović M, Paunović D, Giurato G, Filipović B, Subotić A, Todorović S, Simonović A. Plant regeneration in leaf culture of Centaurium erythraea Rafn. Part 3: de novo transcriptome assembly and validation of housekeeping genes for studies of in vitro morphogenesis. in Plant Cell, Tissue and Organ Culture. 2020;141(2):417-433.
doi:10.1007/s11240-020-01801-w .

Ćuković, Katarina, Dragićević, Milan, Bogdanović, Milica, Paunović, Danijela, Giurato, Giorgio, Filipović, Biljana, Subotić, Angelina, Todorović, Slađana, Simonović, Ana, "Plant regeneration in leaf culture of Centaurium erythraea Rafn. Part 3: de novo transcriptome assembly and validation of housekeeping genes for studies of in vitro morphogenesis" in Plant Cell, Tissue and Organ Culture, 141, no. 2 (2020):417-433,
https://doi.org/10.1007/s11240-020-01801-w . .

TY  - CONF
AU  - Ćuković, Katarina
AU  - Dragićević, Milan
AU  - Todorović, Slađana
AU  - Bogdanović, Milica
AU  - Simonović, Ana
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5439
AB  - Centaurium erythraea Rafn. (Gentianaceae) represents a well-known medicinal plant rich in secondary metabolites and an appropriate model system for studying developmental biology due to its great developmental plasticity in vitro. One of the most unique manifestations of this characteristic in plants is somatic embryogenesis (SE), the development of embryos from somatic cells. SE initiation and progressing of the embryo through different developmental stages involves gene reprogramming and differential expression of numerous genes. Our current research focuses on characterizing novel SE-related genes in centaury tissues using next-generation RNA sequencing data and quantitative real-time PCR (qPCR). Six different centaury tissues, including embryogenic calli, globular and cotiledonary somatic embryos were subjected to ultra-high-throughput sequencing on an Illumina HiSeq 2500 platform, followed by de novo transcriptome assembly. FPKM (fragments per kilobase of exon model per million reads mapped) was used to evaluate in silico expression of functionally annotated transcripts. The generated transcriptome data were subsequently used for discovering genes with potential roles in centaury SE. Potential SE markers were excavated focusing on transcripts with greater than eight fold change in FPKM values in embryogenic tissues compared to non-embryogenic ones. Based on these criteria, twenty transcripts were chosen,
four with completely unknown function. Expression analysis of selected genes was carried out by qPCR using specific primers in different embryogenic and non-embryogenic centaury tissues. Identified genes with differential expression during SE will be further characterized by overexpression and silencing in centaury.
PB  - East Sarajevo: Faculty of Agriculture
C3  - Book of abstracts: X International Scientific Agriculture Symposium: Agrosym 2019; 2019 Oct 3-6; Jahorina, Republika Srpska, Bosnia and Herzegovina
T1  - Selection of differentially expressed genes in Centaurium erythraea Rafn. during in vitro somatic embryogenesis
SP  - 226
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5439
ER  - 

@conference{
author = "Ćuković, Katarina and Dragićević, Milan and Todorović, Slađana and Bogdanović, Milica and Simonović, Ana",
year = "2019",
abstract = "Centaurium erythraea Rafn. (Gentianaceae) represents a well-known medicinal plant rich in secondary metabolites and an appropriate model system for studying developmental biology due to its great developmental plasticity in vitro. One of the most unique manifestations of this characteristic in plants is somatic embryogenesis (SE), the development of embryos from somatic cells. SE initiation and progressing of the embryo through different developmental stages involves gene reprogramming and differential expression of numerous genes. Our current research focuses on characterizing novel SE-related genes in centaury tissues using next-generation RNA sequencing data and quantitative real-time PCR (qPCR). Six different centaury tissues, including embryogenic calli, globular and cotiledonary somatic embryos were subjected to ultra-high-throughput sequencing on an Illumina HiSeq 2500 platform, followed by de novo transcriptome assembly. FPKM (fragments per kilobase of exon model per million reads mapped) was used to evaluate in silico expression of functionally annotated transcripts. The generated transcriptome data were subsequently used for discovering genes with potential roles in centaury SE. Potential SE markers were excavated focusing on transcripts with greater than eight fold change in FPKM values in embryogenic tissues compared to non-embryogenic ones. Based on these criteria, twenty transcripts were chosen,
four with completely unknown function. Expression analysis of selected genes was carried out by qPCR using specific primers in different embryogenic and non-embryogenic centaury tissues. Identified genes with differential expression during SE will be further characterized by overexpression and silencing in centaury.",
publisher = "East Sarajevo: Faculty of Agriculture",
journal = "Book of abstracts: X International Scientific Agriculture Symposium: Agrosym 2019; 2019 Oct 3-6; Jahorina, Republika Srpska, Bosnia and Herzegovina",
title = "Selection of differentially expressed genes in Centaurium erythraea Rafn. during in vitro somatic embryogenesis",
pages = "226",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5439"
}

Ćuković, K., Dragićević, M., Todorović, S., Bogdanović, M.,& Simonović, A.. (2019). Selection of differentially expressed genes in Centaurium erythraea Rafn. during in vitro somatic embryogenesis. in Book of abstracts: X International Scientific Agriculture Symposium: Agrosym 2019; 2019 Oct 3-6; Jahorina, Republika Srpska, Bosnia and Herzegovina
East Sarajevo: Faculty of Agriculture., 226.
https://hdl.handle.net/21.15107/rcub_ibiss_5439

Ćuković K, Dragićević M, Todorović S, Bogdanović M, Simonović A. Selection of differentially expressed genes in Centaurium erythraea Rafn. during in vitro somatic embryogenesis. in Book of abstracts: X International Scientific Agriculture Symposium: Agrosym 2019; 2019 Oct 3-6; Jahorina, Republika Srpska, Bosnia and Herzegovina. 2019;:226.
https://hdl.handle.net/21.15107/rcub_ibiss_5439 .

Ćuković, Katarina, Dragićević, Milan, Todorović, Slađana, Bogdanović, Milica, Simonović, Ana, "Selection of differentially expressed genes in Centaurium erythraea Rafn. during in vitro somatic embryogenesis" in Book of abstracts: X International Scientific Agriculture Symposium: Agrosym 2019; 2019 Oct 3-6; Jahorina, Republika Srpska, Bosnia and Herzegovina (2019):226,
https://hdl.handle.net/21.15107/rcub_ibiss_5439 .

TY  - JOUR
AU  - Dragićević, Milan
AU  - Ćuković, Katarina
AU  - Zdravković-Korać, Snežana
AU  - Simonović, Ana
AU  - Bogdanović, Milica
AU  - Todorović, Slađana
PY  - 2019
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641900026D
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/4099
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3568
AB  - Glutamine synthetase (E.C. 6.3.1.2) is a key enzyme of plant nitrogen metabolism that assimilates ammonia into glutamine. The Arabidopsis thaliana genome encodes one chloroplastic (GLN2) and five cytosolic (GLN1;1 – GLN1;5) isoforms with different expression patterns, kinetic properties, regulation and functions. Physiological roles of different isoforms have been elucidated mainly by studying knockout mutants. However, the role of GLN1;5, which is expressed in dry seeds, remains unknown. To clarifty the function of GLN1;5, we studied a GLN1;5 knockout line (GLN1;5KO) homozygous for T-DNA insertion within the GLN1;5. GLN1;5 deficiency results in a phenotype with slightly delayed bolting and fewer siliques. The dry weight of GLN1;5KO seeds was 73.3% of wild-type (WT) seed weight, with seed length 90.9% of WT seeds. Finally, only 18.33% of the mutant seeds germinated in water within 10 days in comparison to 34.67% of WT seeds. KNO3 strongly stimulated germination of both GLN1;5KO and WT seeds, while germination in the presence of increasing NH4Cl concentrations potentiated the differences between the two genotypes. It can be concluded that GLN1;5 activity supports silique development and grain filling and that it has a role in ammonium reassimilation in the seed, as well as assimilation and/or detoxification of ammonium from the environment.
T2  - Archives of Biological Sciences
T1  - Elucidation of the role of glutamine synthetase seed isoform GLN1;5 in Arabidopsis thaliana (L.) with a reverse genetics approach
IS  - 3
VL  - 71
DO  - 10.2298/ABS190315026D
SP  - 443
EP  - 453
ER  - 

@article{
author = "Dragićević, Milan and Ćuković, Katarina and Zdravković-Korać, Snežana and Simonović, Ana and Bogdanović, Milica and Todorović, Slađana",
year = "2019",
abstract = "Glutamine synthetase (E.C. 6.3.1.2) is a key enzyme of plant nitrogen metabolism that assimilates ammonia into glutamine. The Arabidopsis thaliana genome encodes one chloroplastic (GLN2) and five cytosolic (GLN1;1 – GLN1;5) isoforms with different expression patterns, kinetic properties, regulation and functions. Physiological roles of different isoforms have been elucidated mainly by studying knockout mutants. However, the role of GLN1;5, which is expressed in dry seeds, remains unknown. To clarifty the function of GLN1;5, we studied a GLN1;5 knockout line (GLN1;5KO) homozygous for T-DNA insertion within the GLN1;5. GLN1;5 deficiency results in a phenotype with slightly delayed bolting and fewer siliques. The dry weight of GLN1;5KO seeds was 73.3% of wild-type (WT) seed weight, with seed length 90.9% of WT seeds. Finally, only 18.33% of the mutant seeds germinated in water within 10 days in comparison to 34.67% of WT seeds. KNO3 strongly stimulated germination of both GLN1;5KO and WT seeds, while germination in the presence of increasing NH4Cl concentrations potentiated the differences between the two genotypes. It can be concluded that GLN1;5 activity supports silique development and grain filling and that it has a role in ammonium reassimilation in the seed, as well as assimilation and/or detoxification of ammonium from the environment.",
journal = "Archives of Biological Sciences",
title = "Elucidation of the role of glutamine synthetase seed isoform GLN1;5 in Arabidopsis thaliana (L.) with a reverse genetics approach",
number = "3",
volume = "71",
doi = "10.2298/ABS190315026D",
pages = "443-453"
}

Dragićević, M., Ćuković, K., Zdravković-Korać, S., Simonović, A., Bogdanović, M.,& Todorović, S.. (2019). Elucidation of the role of glutamine synthetase seed isoform GLN1;5 in Arabidopsis thaliana (L.) with a reverse genetics approach. in Archives of Biological Sciences, 71(3), 443-453.
https://doi.org/10.2298/ABS190315026D

Dragićević M, Ćuković K, Zdravković-Korać S, Simonović A, Bogdanović M, Todorović S. Elucidation of the role of glutamine synthetase seed isoform GLN1;5 in Arabidopsis thaliana (L.) with a reverse genetics approach. in Archives of Biological Sciences. 2019;71(3):443-453.
doi:10.2298/ABS190315026D .

Dragićević, Milan, Ćuković, Katarina, Zdravković-Korać, Snežana, Simonović, Ana, Bogdanović, Milica, Todorović, Slađana, "Elucidation of the role of glutamine synthetase seed isoform GLN1;5 in Arabidopsis thaliana (L.) with a reverse genetics approach" in Archives of Biological Sciences, 71, no. 3 (2019):443-453,
https://doi.org/10.2298/ABS190315026D . .