TY  - JOUR
AU  - Urošević, Aleksandar
AU  - Budečević, Sanja
AU  - Ljubisavljević, Katarina
AU  - Tomašević Kolarov, Nataša
AU  - Ajduković, Maja
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6571
AB  - The modular organization of tetrapod paired limbs and girdles, influenced by the expression of Hox genes is one of the primary driving forces of the evolution of animal locomotion. The increased morphological diversification of the paired limbs is correlated with reduced between-limb covariation, while correlation within the elements is usually higher than between the elements. The tailed amphibians, such as Lissotriton newts, have a biphasic lifestyle with aquatic and terrestrial environments imposing different constraints on limb skeleton. By employing the methods of computerized microtomography and 3D geometric morphometrics, we explored the pattern of morphological variation, disparity, modularity and morphological integration in the proximal parts of the anterior limbs of six species of Eurasian small bodied newts. Although the species significantly differ in limb shape, there is a great overlap in morphology of scapula and humerus, and there are no differences in morphological disparity. For the scapula, the shape differences related to the duration of the aquatic period are in length, depth and curvature. The shape of the humerus is not affected by the length of aquatic period, and shape differences between the species are related to robustness of the body. The length of aquatic period has statistically supported phylogenetic signal. The scapula and humerus are structures of varying modularity. For the humerus, the strongest support on the phylogenetic level was for the capitulum/shaft hypothesis, which can also be interpreted as functional modularity. For the scapula, the greatest support was for the antero-posterior hypothesis of modularity in case of Lissotriton vulgaris, which can be explained by different functional roles and muscle insertion patterns, while there was no phylogenetic modularity. The modularity patterns seem to correspond with the general tetrapod pattern, with modularity being more pronounced in the distal structure. The future research should include more salamandrid taxa with different habitat preferences and both adult and larval stages, in order to explore how size, phylogeny and ecology affect the morphology and covariation patterns of limbs.
PB  - John Wiley and Sons
T2  - Journal of Anatomy
T1  - Morphological variation, modularity and integration in the scapula and humerus of Lissotriton newts
DO  - 10.1111/joa.14030
ER  - 

@article{
author = "Urošević, Aleksandar and Budečević, Sanja and Ljubisavljević, Katarina and Tomašević Kolarov, Nataša and Ajduković, Maja",
year = "2024",
abstract = "The modular organization of tetrapod paired limbs and girdles, influenced by the expression of Hox genes is one of the primary driving forces of the evolution of animal locomotion. The increased morphological diversification of the paired limbs is correlated with reduced between-limb covariation, while correlation within the elements is usually higher than between the elements. The tailed amphibians, such as Lissotriton newts, have a biphasic lifestyle with aquatic and terrestrial environments imposing different constraints on limb skeleton. By employing the methods of computerized microtomography and 3D geometric morphometrics, we explored the pattern of morphological variation, disparity, modularity and morphological integration in the proximal parts of the anterior limbs of six species of Eurasian small bodied newts. Although the species significantly differ in limb shape, there is a great overlap in morphology of scapula and humerus, and there are no differences in morphological disparity. For the scapula, the shape differences related to the duration of the aquatic period are in length, depth and curvature. The shape of the humerus is not affected by the length of aquatic period, and shape differences between the species are related to robustness of the body. The length of aquatic period has statistically supported phylogenetic signal. The scapula and humerus are structures of varying modularity. For the humerus, the strongest support on the phylogenetic level was for the capitulum/shaft hypothesis, which can also be interpreted as functional modularity. For the scapula, the greatest support was for the antero-posterior hypothesis of modularity in case of Lissotriton vulgaris, which can be explained by different functional roles and muscle insertion patterns, while there was no phylogenetic modularity. The modularity patterns seem to correspond with the general tetrapod pattern, with modularity being more pronounced in the distal structure. The future research should include more salamandrid taxa with different habitat preferences and both adult and larval stages, in order to explore how size, phylogeny and ecology affect the morphology and covariation patterns of limbs.",
publisher = "John Wiley and Sons",
journal = "Journal of Anatomy",
title = "Morphological variation, modularity and integration in the scapula and humerus of Lissotriton newts",
doi = "10.1111/joa.14030"
}

Urošević, A., Budečević, S., Ljubisavljević, K., Tomašević Kolarov, N.,& Ajduković, M.. (2024). Morphological variation, modularity and integration in the scapula and humerus of Lissotriton newts. in Journal of Anatomy
John Wiley and Sons..
https://doi.org/10.1111/joa.14030

Urošević A, Budečević S, Ljubisavljević K, Tomašević Kolarov N, Ajduković M. Morphological variation, modularity and integration in the scapula and humerus of Lissotriton newts. in Journal of Anatomy. 2024;.
doi:10.1111/joa.14030 .

Urošević, Aleksandar, Budečević, Sanja, Ljubisavljević, Katarina, Tomašević Kolarov, Nataša, Ajduković, Maja, "Morphological variation, modularity and integration in the scapula and humerus of Lissotriton newts" in Journal of Anatomy (2024),
https://doi.org/10.1111/joa.14030 . .

TY  - JOUR
AU  - Urošević, Aleksandar
AU  - Budečević, Sanja
AU  - Ljubisavljević, Katarina
AU  - Tomašević Kolarov, Nataša
AU  - Ajduković, Maja
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6571
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6572
AB  - The modular organization of tetrapod paired limbs and girdles, influenced by the expression of Hox genes is one of the primary driving forces of the evolution of animal locomotion. The increased morphological diversification of the paired limbs is correlated with reduced between-limb covariation, while correlation within the elements is usually higher than between the elements. The tailed amphibians, such as Lissotriton newts, have a biphasic lifestyle with aquatic and terrestrial environments imposing different constraints on limb skeleton. By employing the methods of computerized microtomography and 3D geometric morphometrics, we explored the pattern of morphological variation, disparity, modularity and morphological integration in the proximal parts of the anterior limbs of six species of Eurasian small bodied newts. Although the species significantly differ in limb shape, there is a great overlap in morphology of scapula and humerus, and there are no differences in morphological disparity. For the scapula, the shape differences related to the duration of the aquatic period are in length, depth and curvature. The shape of the humerus is not affected by the length of aquatic period, and shape differences between the species are related to robustness of the body. The length of aquatic period has statistically supported phylogenetic signal. The scapula and humerus are structures of varying modularity. For the humerus, the strongest support on the phylogenetic level was for the capitulum/shaft hypothesis, which can also be interpreted as functional modularity. For the scapula, the greatest support was for the antero-posterior hypothesis of modularity in case of Lissotriton vulgaris, which can be explained by different functional roles and muscle insertion patterns, while there was no phylogenetic modularity. The modularity patterns seem to correspond with the general tetrapod pattern, with modularity being more pronounced in the distal structure. The future research should include more salamandrid taxa with different habitat preferences and both adult and larval stages, in order to explore how size, phylogeny and ecology affect the morphology and covariation patterns of limbs.
PB  - John Wiley and Sons
T2  - Journal of Anatomy
T1  - Morphological variation, modularity and integration in the scapula and humerus of Lissotriton newts
DO  - 10.1111/joa.14030
ER  - 

@article{
author = "Urošević, Aleksandar and Budečević, Sanja and Ljubisavljević, Katarina and Tomašević Kolarov, Nataša and Ajduković, Maja",
year = "2024",
abstract = "The modular organization of tetrapod paired limbs and girdles, influenced by the expression of Hox genes is one of the primary driving forces of the evolution of animal locomotion. The increased morphological diversification of the paired limbs is correlated with reduced between-limb covariation, while correlation within the elements is usually higher than between the elements. The tailed amphibians, such as Lissotriton newts, have a biphasic lifestyle with aquatic and terrestrial environments imposing different constraints on limb skeleton. By employing the methods of computerized microtomography and 3D geometric morphometrics, we explored the pattern of morphological variation, disparity, modularity and morphological integration in the proximal parts of the anterior limbs of six species of Eurasian small bodied newts. Although the species significantly differ in limb shape, there is a great overlap in morphology of scapula and humerus, and there are no differences in morphological disparity. For the scapula, the shape differences related to the duration of the aquatic period are in length, depth and curvature. The shape of the humerus is not affected by the length of aquatic period, and shape differences between the species are related to robustness of the body. The length of aquatic period has statistically supported phylogenetic signal. The scapula and humerus are structures of varying modularity. For the humerus, the strongest support on the phylogenetic level was for the capitulum/shaft hypothesis, which can also be interpreted as functional modularity. For the scapula, the greatest support was for the antero-posterior hypothesis of modularity in case of Lissotriton vulgaris, which can be explained by different functional roles and muscle insertion patterns, while there was no phylogenetic modularity. The modularity patterns seem to correspond with the general tetrapod pattern, with modularity being more pronounced in the distal structure. The future research should include more salamandrid taxa with different habitat preferences and both adult and larval stages, in order to explore how size, phylogeny and ecology affect the morphology and covariation patterns of limbs.",
publisher = "John Wiley and Sons",
journal = "Journal of Anatomy",
title = "Morphological variation, modularity and integration in the scapula and humerus of Lissotriton newts",
doi = "10.1111/joa.14030"
}

Urošević, A., Budečević, S., Ljubisavljević, K., Tomašević Kolarov, N.,& Ajduković, M.. (2024). Morphological variation, modularity and integration in the scapula and humerus of Lissotriton newts. in Journal of Anatomy
John Wiley and Sons..
https://doi.org/10.1111/joa.14030

Urošević A, Budečević S, Ljubisavljević K, Tomašević Kolarov N, Ajduković M. Morphological variation, modularity and integration in the scapula and humerus of Lissotriton newts. in Journal of Anatomy. 2024;.
doi:10.1111/joa.14030 .

Urošević, Aleksandar, Budečević, Sanja, Ljubisavljević, Katarina, Tomašević Kolarov, Nataša, Ajduković, Maja, "Morphological variation, modularity and integration in the scapula and humerus of Lissotriton newts" in Journal of Anatomy (2024),
https://doi.org/10.1111/joa.14030 . .

TY  - JOUR
AU  - Ajduković, Maja
AU  - Ukropina, Mirela
AU  - Cvijanović, Milena
AU  - Vučić, Tijana
AU  - Ivanović, Ana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5579
AB  - Background: Amphibian skin has been studied for many decades, especially the metamorphic changes in
the skin of frogs. Less attention has been paid to salamander skin. Here, we describe changes in the skin
structure during postembryonic development in a salamandrid species, the Balkan crested newt Triturus
ivanbureschi.
Method: Using traditional histological techniques we examined the skin in the trunk region of three premetamorphic
larval stages (hatchling, mid larval and late larval) and two postmetamorphic stages (juvenile,
just after metamorphosis, and adult).
Results: In larval stages, skin consists only of the epidermis, which gradually develops from the single
epithelial cell layer in hatchlings, to a stratified epidermis with gland nests and characteristic Leydig cells at
the late larval stage. During metamorphosis, Leydig cells disappear, and the dermal layer develops. In
postmetamorphic stages, skin is differentiated on stratified epidermis and the dermis with well-developed
glands. Three types of glands were observed in the skin of the postmetamorphic stages: mucous, granular
and mixed. Gland composition appears to be stage- and sex-specific, with juveniles and adult female being
more similar to each other. In juveniles and adult female, there are a similar proportion of glands in both
dorsal and ventral skin, whereas in adult male granular glands dominated the dorsal skin, while mixed
glands dominated the ventral skin.
Conclusion: Our results provide a baseline for future comparative research of skin anatomy in salamanders.
PB  - Elsevier GmbH.
T2  - Annals of Anatomy - Anatomischer Anzeiger
T1  - Histological changes of the skin during postembryonic development of the crested newt Triturus ivanbureschi (Urodela, Salamandridae)
VL  - 249
DO  - 10.1016/j.aanat.2023.152097
SP  - 152097
ER  - 

@article{
author = "Ajduković, Maja and Ukropina, Mirela and Cvijanović, Milena and Vučić, Tijana and Ivanović, Ana",
year = "2023",
abstract = "Background: Amphibian skin has been studied for many decades, especially the metamorphic changes in
the skin of frogs. Less attention has been paid to salamander skin. Here, we describe changes in the skin
structure during postembryonic development in a salamandrid species, the Balkan crested newt Triturus
ivanbureschi.
Method: Using traditional histological techniques we examined the skin in the trunk region of three premetamorphic
larval stages (hatchling, mid larval and late larval) and two postmetamorphic stages (juvenile,
just after metamorphosis, and adult).
Results: In larval stages, skin consists only of the epidermis, which gradually develops from the single
epithelial cell layer in hatchlings, to a stratified epidermis with gland nests and characteristic Leydig cells at
the late larval stage. During metamorphosis, Leydig cells disappear, and the dermal layer develops. In
postmetamorphic stages, skin is differentiated on stratified epidermis and the dermis with well-developed
glands. Three types of glands were observed in the skin of the postmetamorphic stages: mucous, granular
and mixed. Gland composition appears to be stage- and sex-specific, with juveniles and adult female being
more similar to each other. In juveniles and adult female, there are a similar proportion of glands in both
dorsal and ventral skin, whereas in adult male granular glands dominated the dorsal skin, while mixed
glands dominated the ventral skin.
Conclusion: Our results provide a baseline for future comparative research of skin anatomy in salamanders.",
publisher = "Elsevier GmbH.",
journal = "Annals of Anatomy - Anatomischer Anzeiger",
title = "Histological changes of the skin during postembryonic development of the crested newt Triturus ivanbureschi (Urodela, Salamandridae)",
volume = "249",
doi = "10.1016/j.aanat.2023.152097",
pages = "152097"
}

Ajduković, M., Ukropina, M., Cvijanović, M., Vučić, T.,& Ivanović, A.. (2023). Histological changes of the skin during postembryonic development of the crested newt Triturus ivanbureschi (Urodela, Salamandridae). in Annals of Anatomy - Anatomischer Anzeiger
Elsevier GmbH.., 249, 152097.
https://doi.org/10.1016/j.aanat.2023.152097

Ajduković M, Ukropina M, Cvijanović M, Vučić T, Ivanović A. Histological changes of the skin during postembryonic development of the crested newt Triturus ivanbureschi (Urodela, Salamandridae). in Annals of Anatomy - Anatomischer Anzeiger. 2023;249:152097.
doi:10.1016/j.aanat.2023.152097 .

Ajduković, Maja, Ukropina, Mirela, Cvijanović, Milena, Vučić, Tijana, Ivanović, Ana, "Histological changes of the skin during postembryonic development of the crested newt Triturus ivanbureschi (Urodela, Salamandridae)" in Annals of Anatomy - Anatomischer Anzeiger, 249 (2023):152097,
https://doi.org/10.1016/j.aanat.2023.152097 . .

TY  - JOUR
AU  - Urošević, Aleksandar
AU  - Ajduković, Maja
AU  - Vučić, Tijana
AU  - Scholtes, Stefan
AU  - Arntzen, Jan, W
AU  - Ivanović, Ana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5817
AB  - Serially homologous structures may have complex patterns of regionalization and
morphological integration, influenced by developmental Hox gene expression and
functional constraints. The vertebral column, consisting of a number of repeated,
developmentally constrained, and highly integrated units—vertebrae—is such a
complex serially homologous structure. Functional diversification increases regionalization
and modularity of the vertebral column, particularly in mammals. For
salamanders, three concepts of regionalization of the vertebral column have been
proposed, recognizing one, two, or three presacral regions. Using three‐dimensional
geometric morphometrics on vertebra models acquired with microcomputerized
tomography scanning, we explored the covariation of vertebrae in four closely
related taxa of small‐bodied newts in the genus Lissotriton. The data were analyzed
by segmented linear regression to explore patterns of vertebral regionalization and
by a two‐block partial least squares method to test for morphological integration. All
taxa show a morphological shift posterior to the fifth trunk vertebra, which
corresponds to the two‐region concept. However, morphological integration is
found to be strongest in the mid‐trunk. Taken jointly, these results indicate a highly
integrated presacral vertebral column with a subtle two‐region differentiation. The
results are discussed in relation to specific functional requirements, developmental
and phylogenetic constraints, and specific requirements posed by a biphasic life
cycle and different locomotor modes (swimming vs. walking). Further research
should be conducted on different ontogenetic stages and closely related but
ecologically differentiated species.
PB  - John Wiley and Sons Inc
T2  - Journal of Experimental Zoology Part B - Molecular and Developmental Evolution
T1  - Regionalization and morphological integration in the vertebral column of Eurasian small‐bodied newts (Salamandridae: Lissotriton)
VL  - 2023
DO  - 10.1002/jez.b.23205
ER  - 

@article{
author = "Urošević, Aleksandar and Ajduković, Maja and Vučić, Tijana and Scholtes, Stefan and Arntzen, Jan, W and Ivanović, Ana",
year = "2023",
abstract = "Serially homologous structures may have complex patterns of regionalization and
morphological integration, influenced by developmental Hox gene expression and
functional constraints. The vertebral column, consisting of a number of repeated,
developmentally constrained, and highly integrated units—vertebrae—is such a
complex serially homologous structure. Functional diversification increases regionalization
and modularity of the vertebral column, particularly in mammals. For
salamanders, three concepts of regionalization of the vertebral column have been
proposed, recognizing one, two, or three presacral regions. Using three‐dimensional
geometric morphometrics on vertebra models acquired with microcomputerized
tomography scanning, we explored the covariation of vertebrae in four closely
related taxa of small‐bodied newts in the genus Lissotriton. The data were analyzed
by segmented linear regression to explore patterns of vertebral regionalization and
by a two‐block partial least squares method to test for morphological integration. All
taxa show a morphological shift posterior to the fifth trunk vertebra, which
corresponds to the two‐region concept. However, morphological integration is
found to be strongest in the mid‐trunk. Taken jointly, these results indicate a highly
integrated presacral vertebral column with a subtle two‐region differentiation. The
results are discussed in relation to specific functional requirements, developmental
and phylogenetic constraints, and specific requirements posed by a biphasic life
cycle and different locomotor modes (swimming vs. walking). Further research
should be conducted on different ontogenetic stages and closely related but
ecologically differentiated species.",
publisher = "John Wiley and Sons Inc",
journal = "Journal of Experimental Zoology Part B - Molecular and Developmental Evolution",
title = "Regionalization and morphological integration in the vertebral column of Eurasian small‐bodied newts (Salamandridae: Lissotriton)",
volume = "2023",
doi = "10.1002/jez.b.23205"
}

Urošević, A., Ajduković, M., Vučić, T., Scholtes, S., Arntzen, J. W.,& Ivanović, A.. (2023). Regionalization and morphological integration in the vertebral column of Eurasian small‐bodied newts (Salamandridae: Lissotriton). in Journal of Experimental Zoology Part B - Molecular and Developmental Evolution
John Wiley and Sons Inc., 2023.
https://doi.org/10.1002/jez.b.23205

Urošević A, Ajduković M, Vučić T, Scholtes S, Arntzen JW, Ivanović A. Regionalization and morphological integration in the vertebral column of Eurasian small‐bodied newts (Salamandridae: Lissotriton). in Journal of Experimental Zoology Part B - Molecular and Developmental Evolution. 2023;2023.
doi:10.1002/jez.b.23205 .

Urošević, Aleksandar, Ajduković, Maja, Vučić, Tijana, Scholtes, Stefan, Arntzen, Jan, W, Ivanović, Ana, "Regionalization and morphological integration in the vertebral column of Eurasian small‐bodied newts (Salamandridae: Lissotriton)" in Journal of Experimental Zoology Part B - Molecular and Developmental Evolution, 2023 (2023),
https://doi.org/10.1002/jez.b.23205 . .

TY  - JOUR
AU  - Anđelković, Marko
AU  - Mirč, Marko
AU  - Ajduković, Maja
AU  - Cvijanović, Milena
AU  - Vukov, Tanja
AU  - Vučić, Tijana
AU  - Kijanović, Ana
AU  - Urošević, Aleksandar
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5519
AB  - Alpine habitats are exposed to increasing anthropogenic pressure and climate change. The negative impacts can lead to chronic stress that can affect the survival and reproductive success of individuals and even lead to population extinction. In this study, we analyse different morphological and ecological traits and indices of abiotic and biotic stressors (such as head size and shape, fluctuating asymmetry, body condition index, tail autotomy, and population abundance) in alpine and subalpine populations of two lacertid species (Zootoca vivipara and Lacerta agilis) from Serbia and North Macedonia. These lizards live under different conditions: allotopy/syntopy, different anthropogenic pressure, and different levels of habitat protection. We found differences between syntopic and allotopic populations in pileus size, body condition index (in both species), pileus shape, fluctuating asymmetry (in L. agilis), and abundance (in Z. vivipara). Differences between populations under anthropogenic pressure and populations without it were observed in pileus shape, body condition index (in both species), pileus size, fluctuating asymmetry, tail autotomy and abundance (in L. agilis). On the basis of our results, it is necessary to include other stress indicators in addition to fluctuating asymmetry to quickly observe and quantify the negative effects of threat factors and apply protective measures.
PB  - Basel: MDPI
T2  - Symmetry
T1  - Is Fluctuating Asymmetry a Sufficient Indicator of Stress Level in Two Lizard Species (Zootoca vivipara and Lacerta agilis) from Alpine Habitats?
IS  - 3
VL  - 15
DO  - 10.3390/sym15030721
SP  - 721
ER  - 

@article{
author = "Anđelković, Marko and Mirč, Marko and Ajduković, Maja and Cvijanović, Milena and Vukov, Tanja and Vučić, Tijana and Kijanović, Ana and Urošević, Aleksandar",
year = "2023",
abstract = "Alpine habitats are exposed to increasing anthropogenic pressure and climate change. The negative impacts can lead to chronic stress that can affect the survival and reproductive success of individuals and even lead to population extinction. In this study, we analyse different morphological and ecological traits and indices of abiotic and biotic stressors (such as head size and shape, fluctuating asymmetry, body condition index, tail autotomy, and population abundance) in alpine and subalpine populations of two lacertid species (Zootoca vivipara and Lacerta agilis) from Serbia and North Macedonia. These lizards live under different conditions: allotopy/syntopy, different anthropogenic pressure, and different levels of habitat protection. We found differences between syntopic and allotopic populations in pileus size, body condition index (in both species), pileus shape, fluctuating asymmetry (in L. agilis), and abundance (in Z. vivipara). Differences between populations under anthropogenic pressure and populations without it were observed in pileus shape, body condition index (in both species), pileus size, fluctuating asymmetry, tail autotomy and abundance (in L. agilis). On the basis of our results, it is necessary to include other stress indicators in addition to fluctuating asymmetry to quickly observe and quantify the negative effects of threat factors and apply protective measures.",
publisher = "Basel: MDPI",
journal = "Symmetry",
title = "Is Fluctuating Asymmetry a Sufficient Indicator of Stress Level in Two Lizard Species (Zootoca vivipara and Lacerta agilis) from Alpine Habitats?",
number = "3",
volume = "15",
doi = "10.3390/sym15030721",
pages = "721"
}

Anđelković, M., Mirč, M., Ajduković, M., Cvijanović, M., Vukov, T., Vučić, T., Kijanović, A.,& Urošević, A.. (2023). Is Fluctuating Asymmetry a Sufficient Indicator of Stress Level in Two Lizard Species (Zootoca vivipara and Lacerta agilis) from Alpine Habitats?. in Symmetry
Basel: MDPI., 15(3), 721.
https://doi.org/10.3390/sym15030721

Anđelković M, Mirč M, Ajduković M, Cvijanović M, Vukov T, Vučić T, Kijanović A, Urošević A. Is Fluctuating Asymmetry a Sufficient Indicator of Stress Level in Two Lizard Species (Zootoca vivipara and Lacerta agilis) from Alpine Habitats?. in Symmetry. 2023;15(3):721.
doi:10.3390/sym15030721 .

Anđelković, Marko, Mirč, Marko, Ajduković, Maja, Cvijanović, Milena, Vukov, Tanja, Vučić, Tijana, Kijanović, Ana, Urošević, Aleksandar, "Is Fluctuating Asymmetry a Sufficient Indicator of Stress Level in Two Lizard Species (Zootoca vivipara and Lacerta agilis) from Alpine Habitats?" in Symmetry, 15, no. 3 (2023):721,
https://doi.org/10.3390/sym15030721 . .

TY  - JOUR
AU  - Cvijanović, Milena
AU  - Ajduković, Maja
AU  - Arntzen, Jan Willem
AU  - Ivanović, Ana
AU  - Vučić, Tijana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5474
AB  - Micro-computed tomography is a powerful tool toward the detailed reconstruction of internal and external morphology, in particular for ossified and other dense tissues. Here, we document and compare the level of calcification in the skin of the head and the parotoids (the external skin glands) in males and females of common and spined toads, Bufo bufo and B. spinosus. In some anurans, including Bufo species, a specific acellular calcified tissue layer within the dermis has been documented (the Eberth-Katschenko, or EK-layer). By a combination of micro-computed tomography and classical histology, we detected additional calcium deposits located in the dermal layer stratum spongiosum, positioned above the EK-layer. We showed that the level of calcification and the presence of additional calcium deposits are size and sex related, increasing in the order B. bufo males, B. spinosus males, B. bufo females to B. spinosus females. The last of these groups is the least variable. Bufo spinosus females have dense calcium deposits in the parotoids and the dorsal and ventral skin. Three-dimensional volume renderings and cross-sectional slices obtained by micro-CT scanning indicate that this approach is a promising technique for further studies on bufonid skin anatomy and geographic variation in skin calcification.
PB  - John Wiley and Sons Inc
T2  - The Anatomical Record
T1  - Ladies in armor: A micro-computed tomographic study of skin calcification in European toads (genus Bufo)
DO  - 10.1002/ar.25170
ER  - 

@article{
author = "Cvijanović, Milena and Ajduković, Maja and Arntzen, Jan Willem and Ivanović, Ana and Vučić, Tijana",
year = "2023",
abstract = "Micro-computed tomography is a powerful tool toward the detailed reconstruction of internal and external morphology, in particular for ossified and other dense tissues. Here, we document and compare the level of calcification in the skin of the head and the parotoids (the external skin glands) in males and females of common and spined toads, Bufo bufo and B. spinosus. In some anurans, including Bufo species, a specific acellular calcified tissue layer within the dermis has been documented (the Eberth-Katschenko, or EK-layer). By a combination of micro-computed tomography and classical histology, we detected additional calcium deposits located in the dermal layer stratum spongiosum, positioned above the EK-layer. We showed that the level of calcification and the presence of additional calcium deposits are size and sex related, increasing in the order B. bufo males, B. spinosus males, B. bufo females to B. spinosus females. The last of these groups is the least variable. Bufo spinosus females have dense calcium deposits in the parotoids and the dorsal and ventral skin. Three-dimensional volume renderings and cross-sectional slices obtained by micro-CT scanning indicate that this approach is a promising technique for further studies on bufonid skin anatomy and geographic variation in skin calcification.",
publisher = "John Wiley and Sons Inc",
journal = "The Anatomical Record",
title = "Ladies in armor: A micro-computed tomographic study of skin calcification in European toads (genus Bufo)",
doi = "10.1002/ar.25170"
}

Cvijanović, M., Ajduković, M., Arntzen, J. W., Ivanović, A.,& Vučić, T.. (2023). Ladies in armor: A micro-computed tomographic study of skin calcification in European toads (genus Bufo). in The Anatomical Record
John Wiley and Sons Inc..
https://doi.org/10.1002/ar.25170

Cvijanović M, Ajduković M, Arntzen JW, Ivanović A, Vučić T. Ladies in armor: A micro-computed tomographic study of skin calcification in European toads (genus Bufo). in The Anatomical Record. 2023;.
doi:10.1002/ar.25170 .

Cvijanović, Milena, Ajduković, Maja, Arntzen, Jan Willem, Ivanović, Ana, Vučić, Tijana, "Ladies in armor: A micro-computed tomographic study of skin calcification in European toads (genus Bufo)" in The Anatomical Record (2023),
https://doi.org/10.1002/ar.25170 . .

TY  - JOUR
AU  - Ajduković, Maja
AU  - Vučić, Tijana
AU  - Cvijanović, Milena
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5032
AB  - Amphibians are useful bioindicators for monitoring aquatic health and the influence of xenobiotics such as endocrine disrupting chemicals. Because aquatic ecosystems experience the majority of global pollution, aquatic organisms are most exposed and vulnerable to endocrine disruptors. Furthermore, penetration of endocrine disruptors into aquatic organisms especially in amphibians is even easier because of more permeable skin, resulting in high bioavailability and bioaccumulation of chemicals. One of the most potent endocrine disruptors is thiourea, which chemically blocks the synthesis of thyroid hormones and prevents metamorphosis in amphibians. We investigated the influence of thiourea on histomorphology of the thyroid gland in Triturus newts at the metamorphic stage, when thyroid hormone concentrations should reach their maximum level. Chronic exposure to thiourea induced hypertrophy and hyperplasia of follicular cells as well as a significant reduction of interstitial tissue. The intensity of the thyroglobulin immunostaining signal significantly decreases upon chronic exposure to thiourea. Successful cross-reactivity of human primary antibody in immunochemical detection of thyroglobulin in Urodela confirms potential homology in thyroglobulin structure throughout the vertebrates.
PB  - Munich: Elsevier GmbH
T2  - Zoology
T1  - Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts
VL  - 155
DO  - 10.1016/j.zool.2022.126050
SP  - 126050
ER  - 

@article{
author = "Ajduković, Maja and Vučić, Tijana and Cvijanović, Milena and Filipović, Branko and Šošić-Jurjević, Branka ",
year = "2022",
abstract = "Amphibians are useful bioindicators for monitoring aquatic health and the influence of xenobiotics such as endocrine disrupting chemicals. Because aquatic ecosystems experience the majority of global pollution, aquatic organisms are most exposed and vulnerable to endocrine disruptors. Furthermore, penetration of endocrine disruptors into aquatic organisms especially in amphibians is even easier because of more permeable skin, resulting in high bioavailability and bioaccumulation of chemicals. One of the most potent endocrine disruptors is thiourea, which chemically blocks the synthesis of thyroid hormones and prevents metamorphosis in amphibians. We investigated the influence of thiourea on histomorphology of the thyroid gland in Triturus newts at the metamorphic stage, when thyroid hormone concentrations should reach their maximum level. Chronic exposure to thiourea induced hypertrophy and hyperplasia of follicular cells as well as a significant reduction of interstitial tissue. The intensity of the thyroglobulin immunostaining signal significantly decreases upon chronic exposure to thiourea. Successful cross-reactivity of human primary antibody in immunochemical detection of thyroglobulin in Urodela confirms potential homology in thyroglobulin structure throughout the vertebrates.",
publisher = "Munich: Elsevier GmbH",
journal = "Zoology",
title = "Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts",
volume = "155",
doi = "10.1016/j.zool.2022.126050",
pages = "126050"
}

Ajduković, M., Vučić, T., Cvijanović, M., Filipović, B.,& Šošić-Jurjević, B.. (2022). Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts. in Zoology
Munich: Elsevier GmbH., 155, 126050.
https://doi.org/10.1016/j.zool.2022.126050

Ajduković M, Vučić T, Cvijanović M, Filipović B, Šošić-Jurjević B. Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts. in Zoology. 2022;155:126050.
doi:10.1016/j.zool.2022.126050 .

Ajduković, Maja, Vučić, Tijana, Cvijanović, Milena, Filipović, Branko, Šošić-Jurjević, Branka , "Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts" in Zoology, 155 (2022):126050,
https://doi.org/10.1016/j.zool.2022.126050 . .

TY  - CONF
AU  - Ajduković, Maja
AU  - Vučić, Tijana
AU  - Cvijanović, Milena
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5227
AB  - Aquatic ecosystems receive the majority of global pollution. Therefore, aquatic
organisms are the most exposed and the most vulnerable as penetration of various
endocrine disruptors is easier, resulting in high bioavailability and bioaccumulation of
chemicals. One of the potent endocrine disruptors (anti-thyroid agents) is thiourea,
which chemically blocks the synthesis of thyroid hormones and prevents
metamorphosis in amphibians. The mechanism of action is very similar for all antithyroid agents via inhibition of thyroid peroxidase enzyme (TPO), thereby inhibiting
iodination of tyrosine residues in thyroglobulin, the oxidative coupling of iodinated
tyrosine and therefore the biosynthesis of the thyroid hormones. We investigated the
influence of two non-lethal concentrations of thiourea (0.05% and 0.1%) on
histomorphology of the thyroid gland in Triturus newts at the metamorphic stage,
when TH concentrations should reach maximum level. Exposure to thiourea induced
hypertrophy and hyperplasia of follicular cells as well as a significant reduction
(p<0.05) of interstitial tissue. Intensity of thyroglobulin immunostaining significantly
decreased (p<0.05) upon both thiourea treatments. Additionally, we tested
mammalian thyroglobulin antibodies for immunohistochemical assessment of the
thyroid follicular tissue in newts. Successful cross-reactivity of human primary
antibody in immunochemical detection of thyroglobulin in Triturus newts in this study
confirms potential homology in protein structure throughout the vertebrates.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
T1  - Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts
SP  - 41
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5227
ER  - 

@conference{
author = "Ajduković, Maja and Vučić, Tijana and Cvijanović, Milena and Filipović, Branko and Šošić-Jurjević, Branka ",
year = "2022",
abstract = "Aquatic ecosystems receive the majority of global pollution. Therefore, aquatic
organisms are the most exposed and the most vulnerable as penetration of various
endocrine disruptors is easier, resulting in high bioavailability and bioaccumulation of
chemicals. One of the potent endocrine disruptors (anti-thyroid agents) is thiourea,
which chemically blocks the synthesis of thyroid hormones and prevents
metamorphosis in amphibians. The mechanism of action is very similar for all antithyroid agents via inhibition of thyroid peroxidase enzyme (TPO), thereby inhibiting
iodination of tyrosine residues in thyroglobulin, the oxidative coupling of iodinated
tyrosine and therefore the biosynthesis of the thyroid hormones. We investigated the
influence of two non-lethal concentrations of thiourea (0.05% and 0.1%) on
histomorphology of the thyroid gland in Triturus newts at the metamorphic stage,
when TH concentrations should reach maximum level. Exposure to thiourea induced
hypertrophy and hyperplasia of follicular cells as well as a significant reduction
(p<0.05) of interstitial tissue. Intensity of thyroglobulin immunostaining significantly
decreased (p<0.05) upon both thiourea treatments. Additionally, we tested
mammalian thyroglobulin antibodies for immunohistochemical assessment of the
thyroid follicular tissue in newts. Successful cross-reactivity of human primary
antibody in immunochemical detection of thyroglobulin in Triturus newts in this study
confirms potential homology in protein structure throughout the vertebrates.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia",
title = "Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts",
pages = "41",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5227"
}

Ajduković, M., Vučić, T., Cvijanović, M., Filipović, B.,& Šošić-Jurjević, B.. (2022). Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 41.
https://hdl.handle.net/21.15107/rcub_ibiss_5227

Ajduković M, Vučić T, Cvijanović M, Filipović B, Šošić-Jurjević B. Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. 2022;:41.
https://hdl.handle.net/21.15107/rcub_ibiss_5227 .

Ajduković, Maja, Vučić, Tijana, Cvijanović, Milena, Filipović, Branko, Šošić-Jurjević, Branka , "Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts" in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia (2022):41,
https://hdl.handle.net/21.15107/rcub_ibiss_5227 .

TY  - JOUR
AU  - Vučić, Tijana
AU  - Ivanović, Ana
AU  - Ajduković, Maja
AU  - Bajler, Nikola
AU  - Cvijanović, Milena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4866
AB  - Two large-bodied newt species, Triturus ivanbureschi and T. macedonicus, hybridize in nature across the Balkan Peninsula. Consequences of hybridization upon secondary contact of two species include species displacement and asymmetrical introgression of T. ivanbureschi mtDNA. We set an experimental reciprocal cross of parental species and obtained two genotypes of F1 hybrids (with T. ivanbureschi or T. macedonicus mtDNA). When hybrids attained sexual maturity, they were engaged in mutual crossings and backcrossing with parental species. We followed reproductive traits over two successive years. Our main aim was to explore the reproductive success of F1 females carrying different parental mtDNA. Additionally, we tested for differences in reproductive success within female genotypes depending on the crossing with various male genotypes (hybrids or parental species). Both female genotypes had similar oviposition periods, number of laid eggs and hatched larvae but different body and egg sizes. Overall reproductive success (percentage of egg-laying females and viability of embryos) was similar for both genotypes. The type of crossing led to some differences in reproductive success within female genotypes. The obtained results suggest that processes that led to exclusion of T. macedonicus mtDNA in natural populations may be related to the survival at postembryonic stages of F2 generation or reproductive barriers that emerged in subsequent hybrid generations.
PB  - Basel: MDPI
T2  - Animals
T1  - The reproductive success of Triturus ivanbureschi x T. macedonicus F1 hzbrid females (Amphibia:Salamandridae)
IS  - 4
VL  - 12
DO  - 10.3390/ani12040443
SP  - 443
ER  - 

@article{
author = "Vučić, Tijana and Ivanović, Ana and Ajduković, Maja and Bajler, Nikola and Cvijanović, Milena",
year = "2022",
abstract = "Two large-bodied newt species, Triturus ivanbureschi and T. macedonicus, hybridize in nature across the Balkan Peninsula. Consequences of hybridization upon secondary contact of two species include species displacement and asymmetrical introgression of T. ivanbureschi mtDNA. We set an experimental reciprocal cross of parental species and obtained two genotypes of F1 hybrids (with T. ivanbureschi or T. macedonicus mtDNA). When hybrids attained sexual maturity, they were engaged in mutual crossings and backcrossing with parental species. We followed reproductive traits over two successive years. Our main aim was to explore the reproductive success of F1 females carrying different parental mtDNA. Additionally, we tested for differences in reproductive success within female genotypes depending on the crossing with various male genotypes (hybrids or parental species). Both female genotypes had similar oviposition periods, number of laid eggs and hatched larvae but different body and egg sizes. Overall reproductive success (percentage of egg-laying females and viability of embryos) was similar for both genotypes. The type of crossing led to some differences in reproductive success within female genotypes. The obtained results suggest that processes that led to exclusion of T. macedonicus mtDNA in natural populations may be related to the survival at postembryonic stages of F2 generation or reproductive barriers that emerged in subsequent hybrid generations.",
publisher = "Basel: MDPI",
journal = "Animals",
title = "The reproductive success of Triturus ivanbureschi x T. macedonicus F1 hzbrid females (Amphibia:Salamandridae)",
number = "4",
volume = "12",
doi = "10.3390/ani12040443",
pages = "443"
}

Vučić, T., Ivanović, A., Ajduković, M., Bajler, N.,& Cvijanović, M.. (2022). The reproductive success of Triturus ivanbureschi x T. macedonicus F1 hzbrid females (Amphibia:Salamandridae). in Animals
Basel: MDPI., 12(4), 443.
https://doi.org/10.3390/ani12040443

Vučić T, Ivanović A, Ajduković M, Bajler N, Cvijanović M. The reproductive success of Triturus ivanbureschi x T. macedonicus F1 hzbrid females (Amphibia:Salamandridae). in Animals. 2022;12(4):443.
doi:10.3390/ani12040443 .

Vučić, Tijana, Ivanović, Ana, Ajduković, Maja, Bajler, Nikola, Cvijanović, Milena, "The reproductive success of Triturus ivanbureschi x T. macedonicus F1 hzbrid females (Amphibia:Salamandridae)" in Animals, 12, no. 4 (2022):443,
https://doi.org/10.3390/ani12040443 . .

TY  - CONF
AU  - Urošević, Aleksandar
AU  - Ajduković, Maja
AU  - Vučić, Tijana
AU  - Scholtes, Stefan
AU  - Arntzen, Jan
AU  - Ivanović, Ana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5231
AB  - Serially homologous structures, such as the vertebral column, often undergo 
functional and evolutionary diversification and are a good model-system for studies of 
regionalization and morphological integration. We studied these topics in the vertebral 
column (atlas, trunk and sacral vertebrae) of the closely related taxa of small-bodied 
newts – Lissotriton schmidtleri, L. vulgaris ampelensis, L. v. meridionalis and L. v. 
vulgaris, using 3D geometric morphometrics on models that were acquired with micro CT scanning. Two different statistical approaches were employed to test for vertebral 
regionalization and overall morphological integration, namely segmented linear 
regression (SLR) and a partial least squares method (PLS) We observed a common 
pattern of regionalization, with a transition point after the 5th trunk vertebra. It 
corresponds with the antero-posterior transition common for tetrapods. 
Morphological integration, accessed via PLS analysis, is strongest at the 6th and 7th
trunk vertebrae, while the anterior and distal parts of the vertebral column are less 
integrated. The PLS analysis of the asymmetric component of shape variation revealed 
a weak integration, statistically significant only among subsequent trunk vertebrae. In 
summary, the vertebral column of the closely related Lissotriton newts is subtly 
regionalized, while being morphologically integrated overall. There is a complex 
relationship between regionalization and morphological integration of the vertebral 
column, most likely influenced by the newt’s bi-phasic life cycle that instigates different 
functional constraints in the aquatic and terrestrial life stages
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
T1  - Vertebral regionalization vs. morphological integration in Lissotriton newts
SP  - 38
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5231
ER  - 

@conference{
author = "Urošević, Aleksandar and Ajduković, Maja and Vučić, Tijana and Scholtes, Stefan and Arntzen, Jan and Ivanović, Ana",
year = "2022",
abstract = "Serially homologous structures, such as the vertebral column, often undergo 
functional and evolutionary diversification and are a good model-system for studies of 
regionalization and morphological integration. We studied these topics in the vertebral 
column (atlas, trunk and sacral vertebrae) of the closely related taxa of small-bodied 
newts – Lissotriton schmidtleri, L. vulgaris ampelensis, L. v. meridionalis and L. v. 
vulgaris, using 3D geometric morphometrics on models that were acquired with micro CT scanning. Two different statistical approaches were employed to test for vertebral 
regionalization and overall morphological integration, namely segmented linear 
regression (SLR) and a partial least squares method (PLS) We observed a common 
pattern of regionalization, with a transition point after the 5th trunk vertebra. It 
corresponds with the antero-posterior transition common for tetrapods. 
Morphological integration, accessed via PLS analysis, is strongest at the 6th and 7th
trunk vertebrae, while the anterior and distal parts of the vertebral column are less 
integrated. The PLS analysis of the asymmetric component of shape variation revealed 
a weak integration, statistically significant only among subsequent trunk vertebrae. In 
summary, the vertebral column of the closely related Lissotriton newts is subtly 
regionalized, while being morphologically integrated overall. There is a complex 
relationship between regionalization and morphological integration of the vertebral 
column, most likely influenced by the newt’s bi-phasic life cycle that instigates different 
functional constraints in the aquatic and terrestrial life stages",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia",
title = "Vertebral regionalization vs. morphological integration in Lissotriton newts",
pages = "38",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5231"
}

Urošević, A., Ajduković, M., Vučić, T., Scholtes, S., Arntzen, J.,& Ivanović, A.. (2022). Vertebral regionalization vs. morphological integration in Lissotriton newts. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 38.
https://hdl.handle.net/21.15107/rcub_ibiss_5231

Urošević A, Ajduković M, Vučić T, Scholtes S, Arntzen J, Ivanović A. Vertebral regionalization vs. morphological integration in Lissotriton newts. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. 2022;:38.
https://hdl.handle.net/21.15107/rcub_ibiss_5231 .

Urošević, Aleksandar, Ajduković, Maja, Vučić, Tijana, Scholtes, Stefan, Arntzen, Jan, Ivanović, Ana, "Vertebral regionalization vs. morphological integration in Lissotriton newts" in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia (2022):38,
https://hdl.handle.net/21.15107/rcub_ibiss_5231 .

TY  - CONF
AU  - Drobnjaković, Marija
AU  - Ivanović, Ana
AU  - Bugarčić, Marko
AU  - Cvijanović, Milena
AU  - Ajduković, Maja
AU  - Vučić, Tijana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5230
AB  - Staging tables based on the external morphological characteristics are important 
for the needs of comparative embryological studies as they represent a baseline. Large bodied newts (Triturus spp.) have a transparent mucoid capsule that enables insight 
into the external morphology of the embryo during development. Triturus newts are 
characterized by the presence of chromosome 1 syndrome where about 50% of the 
offspring stops developing and dies during the mid embryonic period on the tail bud 
stage. In this study, we focused on the normal development of Balkan crested newt 
(Triturus ivanbureschi). During the breeding season animals were kept in the yard of 
the institute, in plastic tubs (from 200 to 400 liters), covered with a mosquito net that 
allowed natural day-night regime. Plastic strips were provided as an imitation of 
underwater vegetation, on which the females laid their eggs. Eggs were collected every 
morning and moved to Petri dishes at the controlled experimental conditions. The 
embryos were kept in the laboratory at a temperature of 20°C. Photographs and time lapse recordings of developing embryos were taken every day at the same time. 
Compared to already published staging tables for newts and based on the appearance 
of certain morphological structures, such as gills, extremities, balancers, etc., we
distinguished 37 different stages of normal embryonic development of T. ivanbureshi. 
Still, differences in external morphology due to the balanced lethal chromosome 1 
syndrome need further research
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
T1  - Developmental stages of Triturus ivanbureschi
VL  - 260
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5230
ER  - 

@conference{
author = "Drobnjaković, Marija and Ivanović, Ana and Bugarčić, Marko and Cvijanović, Milena and Ajduković, Maja and Vučić, Tijana",
year = "2022",
abstract = "Staging tables based on the external morphological characteristics are important 
for the needs of comparative embryological studies as they represent a baseline. Large bodied newts (Triturus spp.) have a transparent mucoid capsule that enables insight 
into the external morphology of the embryo during development. Triturus newts are 
characterized by the presence of chromosome 1 syndrome where about 50% of the 
offspring stops developing and dies during the mid embryonic period on the tail bud 
stage. In this study, we focused on the normal development of Balkan crested newt 
(Triturus ivanbureschi). During the breeding season animals were kept in the yard of 
the institute, in plastic tubs (from 200 to 400 liters), covered with a mosquito net that 
allowed natural day-night regime. Plastic strips were provided as an imitation of 
underwater vegetation, on which the females laid their eggs. Eggs were collected every 
morning and moved to Petri dishes at the controlled experimental conditions. The 
embryos were kept in the laboratory at a temperature of 20°C. Photographs and time lapse recordings of developing embryos were taken every day at the same time. 
Compared to already published staging tables for newts and based on the appearance 
of certain morphological structures, such as gills, extremities, balancers, etc., we
distinguished 37 different stages of normal embryonic development of T. ivanbureshi. 
Still, differences in external morphology due to the balanced lethal chromosome 1 
syndrome need further research",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia",
title = "Developmental stages of Triturus ivanbureschi",
volume = "260",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5230"
}

Drobnjaković, M., Ivanović, A., Bugarčić, M., Cvijanović, M., Ajduković, M.,& Vučić, T.. (2022). Developmental stages of Triturus ivanbureschi. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 260.
https://hdl.handle.net/21.15107/rcub_ibiss_5230

Drobnjaković M, Ivanović A, Bugarčić M, Cvijanović M, Ajduković M, Vučić T. Developmental stages of Triturus ivanbureschi. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. 2022;260.
https://hdl.handle.net/21.15107/rcub_ibiss_5230 .

Drobnjaković, Marija, Ivanović, Ana, Bugarčić, Marko, Cvijanović, Milena, Ajduković, Maja, Vučić, Tijana, "Developmental stages of Triturus ivanbureschi" in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia, 260 (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_5230 .

TY  - CONF
AU  - Mirč, Marko
AU  - Anđelković, Marko
AU  - Urošević, Aleksandar
AU  - Cvijanović, Milena
AU  - Tomašević Kolarov, Nataša
AU  - Ajduković, Maja
AU  - Kijanović, Ana
AU  - Vučić, Tijana
AU  - Vukov, Tanja
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5229
AB  - National parks are, by definition, areas set aside for wildlife protection, but also 
human recreation. Sometimes, the development of tourism-related infrastructure and 
capacities or resource exploitation can impede the primary function of national parks 
– nature conservation. Baseline and trend monitoring are great tools to assess human 
pressure on nature. Amphibian and reptile species have small ranges and are 
vulnerable to changes in their environment which makes them great bioindicators. 
Here we present the results of five-year monitoring activities of amphibian and reptile 
populations at National Park „Kopaonik“, Serbia. Data on batracho- and herpetofauna 
of NP „Kopaonik“ were limited and outdated, so a detailed survey of species richness, 
habitat conditions, and population trends was greatly needed. Starting from 2018 to 
2022 we described the amphibian and reptile diversity of the national park and 
systematically monitored several habitats, dispersed throughout all three protection 
zones as well as outside protection zones, to assess the habitat quality and population 
trends of selected species. We had an opportunity to see the effect of the international 
travel ban (due to the Covid19) on batracho- and herpetofauna, when much more 
people spent their vacation at NP „Kopaonik“ than usually. Per our results so far, the 
richness of the amphibians and reptiles species of the NP „Kopanik“ is moderate (9 
amphibian and 12 reptile species). The population size of the selected lizard species is 
stable through the years regardless of the protection zone. Amphibian populations are 
also stable, as offsprings of the majority of the species were found every year. However 
human pressure on the amphibian and reptile species is high. Habitat degradation is 
present and some localities are at great risk to be lost
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
T1  - Five years of monitoring amphibian and reptile populations at National Park  „Kopaonik“, Serbia
SP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5229
ER  - 

@conference{
author = "Mirč, Marko and Anđelković, Marko and Urošević, Aleksandar and Cvijanović, Milena and Tomašević Kolarov, Nataša and Ajduković, Maja and Kijanović, Ana and Vučić, Tijana and Vukov, Tanja",
year = "2022",
abstract = "National parks are, by definition, areas set aside for wildlife protection, but also 
human recreation. Sometimes, the development of tourism-related infrastructure and 
capacities or resource exploitation can impede the primary function of national parks 
– nature conservation. Baseline and trend monitoring are great tools to assess human 
pressure on nature. Amphibian and reptile species have small ranges and are 
vulnerable to changes in their environment which makes them great bioindicators. 
Here we present the results of five-year monitoring activities of amphibian and reptile 
populations at National Park „Kopaonik“, Serbia. Data on batracho- and herpetofauna 
of NP „Kopaonik“ were limited and outdated, so a detailed survey of species richness, 
habitat conditions, and population trends was greatly needed. Starting from 2018 to 
2022 we described the amphibian and reptile diversity of the national park and 
systematically monitored several habitats, dispersed throughout all three protection 
zones as well as outside protection zones, to assess the habitat quality and population 
trends of selected species. We had an opportunity to see the effect of the international 
travel ban (due to the Covid19) on batracho- and herpetofauna, when much more 
people spent their vacation at NP „Kopaonik“ than usually. Per our results so far, the 
richness of the amphibians and reptiles species of the NP „Kopanik“ is moderate (9 
amphibian and 12 reptile species). The population size of the selected lizard species is 
stable through the years regardless of the protection zone. Amphibian populations are 
also stable, as offsprings of the majority of the species were found every year. However 
human pressure on the amphibian and reptile species is high. Habitat degradation is 
present and some localities are at great risk to be lost",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia",
title = "Five years of monitoring amphibian and reptile populations at National Park  „Kopaonik“, Serbia",
pages = "79",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5229"
}

Mirč, M., Anđelković, M., Urošević, A., Cvijanović, M., Tomašević Kolarov, N., Ajduković, M., Kijanović, A., Vučić, T.,& Vukov, T.. (2022). Five years of monitoring amphibian and reptile populations at National Park  „Kopaonik“, Serbia. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 79.
https://hdl.handle.net/21.15107/rcub_ibiss_5229

Mirč M, Anđelković M, Urošević A, Cvijanović M, Tomašević Kolarov N, Ajduković M, Kijanović A, Vučić T, Vukov T. Five years of monitoring amphibian and reptile populations at National Park  „Kopaonik“, Serbia. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. 2022;:79.
https://hdl.handle.net/21.15107/rcub_ibiss_5229 .

Mirč, Marko, Anđelković, Marko, Urošević, Aleksandar, Cvijanović, Milena, Tomašević Kolarov, Nataša, Ajduković, Maja, Kijanović, Ana, Vučić, Tijana, Vukov, Tanja, "Five years of monitoring amphibian and reptile populations at National Park  „Kopaonik“, Serbia" in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia (2022):79,
https://hdl.handle.net/21.15107/rcub_ibiss_5229 .

TY  - CONF
AU  - Bugarčić, Marko
AU  - Ivanović, Ana
AU  - Cvijanović, Milena
AU  - Ajduković, Maja
AU  - Wielstra, Ben
AU  - Vučić, Tijana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5228
AB  - In developmental biology a plethora of studies support the existence of a
conserved stage during the ontogeny of vertebrates – the pharyngula. The
developmental hourglass model predicts that the most conserved morphological
pattern occurs in the middle of embryonic development. We explored external
morphology of the craniofacial region of Triturus newts using 3D geometric
morphometrics. The craniofacial region was selected because it experiences
pleiotropic developmental constrains due to its major role in feeding, respiration and
the housing of substantial parts of nervous and sensory systems. We aimed to uncover
differences in variance between successive developmental stages, because it is
proposed that the more constrained stages should have the lowest variance. In
addition, we explored differences in the craniofacial shape between successive
developmental stages. Stages included start from the moment after the completion of
neurulation and span the mid-tailbud phase. The least amount of variance in shape was
recorded at stage 24 (according to D’Amen and colleagues). In general, this stage is
characterized by changes in overall head shape, the distinction of optical vesicles,
intensive somitogenesis and formation of the tailbud which overgrows the blastopore,
indicating that these processes could be highly constrained. Only significant
craniofacial shape change occurred between this and successive stage. The most
pronounced differences were due to growing of the head region and gill bud balancers.
Optic vesicles became concave due to inductive processes preceding eye formation.
These preliminary results indicate that stage 24 could be the most conserved one
during early ontogeny as it is preceded and followed by stages exhibiting more
variance in shape. Our results indicate that the craniofacial development represents a
suitable model system for testing the hourglass model of development. Larger sample
size, exploration of the entire embryonic development and ontogeny of additional newt
species is needed to confirm our preliminary findings.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
T1  - Craniofacial development of Triturus newts – a suitable model system for  testing the developmental hourglass model
SP  - 42
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5228
ER  - 

@conference{
author = "Bugarčić, Marko and Ivanović, Ana and Cvijanović, Milena and Ajduković, Maja and Wielstra, Ben and Vučić, Tijana",
year = "2022",
abstract = "In developmental biology a plethora of studies support the existence of a
conserved stage during the ontogeny of vertebrates – the pharyngula. The
developmental hourglass model predicts that the most conserved morphological
pattern occurs in the middle of embryonic development. We explored external
morphology of the craniofacial region of Triturus newts using 3D geometric
morphometrics. The craniofacial region was selected because it experiences
pleiotropic developmental constrains due to its major role in feeding, respiration and
the housing of substantial parts of nervous and sensory systems. We aimed to uncover
differences in variance between successive developmental stages, because it is
proposed that the more constrained stages should have the lowest variance. In
addition, we explored differences in the craniofacial shape between successive
developmental stages. Stages included start from the moment after the completion of
neurulation and span the mid-tailbud phase. The least amount of variance in shape was
recorded at stage 24 (according to D’Amen and colleagues). In general, this stage is
characterized by changes in overall head shape, the distinction of optical vesicles,
intensive somitogenesis and formation of the tailbud which overgrows the blastopore,
indicating that these processes could be highly constrained. Only significant
craniofacial shape change occurred between this and successive stage. The most
pronounced differences were due to growing of the head region and gill bud balancers.
Optic vesicles became concave due to inductive processes preceding eye formation.
These preliminary results indicate that stage 24 could be the most conserved one
during early ontogeny as it is preceded and followed by stages exhibiting more
variance in shape. Our results indicate that the craniofacial development represents a
suitable model system for testing the hourglass model of development. Larger sample
size, exploration of the entire embryonic development and ontogeny of additional newt
species is needed to confirm our preliminary findings.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia",
title = "Craniofacial development of Triturus newts – a suitable model system for  testing the developmental hourglass model",
pages = "42",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5228"
}

Bugarčić, M., Ivanović, A., Cvijanović, M., Ajduković, M., Wielstra, B.,& Vučić, T.. (2022). Craniofacial development of Triturus newts – a suitable model system for  testing the developmental hourglass model. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 42.
https://hdl.handle.net/21.15107/rcub_ibiss_5228

Bugarčić M, Ivanović A, Cvijanović M, Ajduković M, Wielstra B, Vučić T. Craniofacial development of Triturus newts – a suitable model system for  testing the developmental hourglass model. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. 2022;:42.
https://hdl.handle.net/21.15107/rcub_ibiss_5228 .

Bugarčić, Marko, Ivanović, Ana, Cvijanović, Milena, Ajduković, Maja, Wielstra, Ben, Vučić, Tijana, "Craniofacial development of Triturus newts – a suitable model system for  testing the developmental hourglass model" in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia (2022):42,
https://hdl.handle.net/21.15107/rcub_ibiss_5228 .

TY  - CONF
AU  - Cvijanović, Milena
AU  - Ajduković, Maja
AU  - Ivanović, Ana
AU  - Arntzen, Jan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5134
AB  - In the skin of many terrestrial anuran taxa, a specific acellular mineralized tissue
layer (the Eberth-Katschenko layer) has been documented. This layer is generally
positioned in between the stratum spongiosum and the stratum compactum of the
dermis and has a role in reducing water loss. Here, we document and compare the
amount of calcium deposition in the skin of the head and the parotoids (the external
skin glands) in males and females of the common toad Bufo bufo and the spined toad B.
spinosus. Bufo bufo and B. spinosus are morphologically similar and both show a
conspicuous sexual dimorphism. Using standard histological techniques, we detected
calcium as an amorphous ‘ground substance’ located in the stratum spongiosum, just
above the Eberth-Katschenko layer. We observed large variability in the number of
calcium deposits between the species and the sexes. Using micro-computed
tomography (micro-CT) we were able to quantify the level of the toad skin calcification.
Bufo spinosus females stand out compared to conspecific males and B. bufo on account
of a strong calcification of the dorsal and ventral skin and the parotoid glands. Species
and sexes significantly differed in the amount of calcium deposits (Fisher’s exact test,
p < 0.001) and pairwise comparisons showed that groups differed from one another (p
< 0.05), suggesting size and sexual dimorphism in these traits. We conclude that microCT scanning is useful for the quantification of calcified structures in the anuran skin,
and keeps a promise for further studies on taxonomic and geographic variation.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
T1  - What is hiding in the Bufo skin? Revealing of the structures in the skin of European toads using standard histological and micro-CT techniques
SP  - 32
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5134
ER  - 

@conference{
author = "Cvijanović, Milena and Ajduković, Maja and Ivanović, Ana and Arntzen, Jan",
year = "2022",
abstract = "In the skin of many terrestrial anuran taxa, a specific acellular mineralized tissue
layer (the Eberth-Katschenko layer) has been documented. This layer is generally
positioned in between the stratum spongiosum and the stratum compactum of the
dermis and has a role in reducing water loss. Here, we document and compare the
amount of calcium deposition in the skin of the head and the parotoids (the external
skin glands) in males and females of the common toad Bufo bufo and the spined toad B.
spinosus. Bufo bufo and B. spinosus are morphologically similar and both show a
conspicuous sexual dimorphism. Using standard histological techniques, we detected
calcium as an amorphous ‘ground substance’ located in the stratum spongiosum, just
above the Eberth-Katschenko layer. We observed large variability in the number of
calcium deposits between the species and the sexes. Using micro-computed
tomography (micro-CT) we were able to quantify the level of the toad skin calcification.
Bufo spinosus females stand out compared to conspecific males and B. bufo on account
of a strong calcification of the dorsal and ventral skin and the parotoid glands. Species
and sexes significantly differed in the amount of calcium deposits (Fisher’s exact test,
p < 0.001) and pairwise comparisons showed that groups differed from one another (p
< 0.05), suggesting size and sexual dimorphism in these traits. We conclude that microCT scanning is useful for the quantification of calcified structures in the anuran skin,
and keeps a promise for further studies on taxonomic and geographic variation.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia",
title = "What is hiding in the Bufo skin? Revealing of the structures in the skin of European toads using standard histological and micro-CT techniques",
pages = "32",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5134"
}

Cvijanović, M., Ajduković, M., Ivanović, A.,& Arntzen, J.. (2022). What is hiding in the Bufo skin? Revealing of the structures in the skin of European toads using standard histological and micro-CT techniques. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 32.
https://hdl.handle.net/21.15107/rcub_ibiss_5134

Cvijanović M, Ajduković M, Ivanović A, Arntzen J. What is hiding in the Bufo skin? Revealing of the structures in the skin of European toads using standard histological and micro-CT techniques. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. 2022;:32.
https://hdl.handle.net/21.15107/rcub_ibiss_5134 .

Cvijanović, Milena, Ajduković, Maja, Ivanović, Ana, Arntzen, Jan, "What is hiding in the Bufo skin? Revealing of the structures in the skin of European toads using standard histological and micro-CT techniques" in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia (2022):32,
https://hdl.handle.net/21.15107/rcub_ibiss_5134 .

TY  - JOUR
AU  - Ajduković, Maja
AU  - Vučić, Tijana
AU  - Cvijanović, Milena
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4251
AB  - Background
In amphibians, thyroid hormone (TH) has a profound role in cranial development, especially in ossification of the late-appearing bones and remodeling of the skull. In the present study, we explored the influence of TH deficiency on bone ossification and resulting skull shape during the ontogeny of Triturus newt hybrid larvae obtained from interspecific crosses between T. ivanbureschi and T. macedonicus.

Methods
Larvae were treated with two concentrations of thiourea (an endocrine disruptor that chemically inhibits synthesis of TH) during the midlarval and late larval periods. Morphological differences of the cranium were assessed at the end of the midlarval period (ontogenetic stage 62) and the metamorphic stage after treatment during the late larval period.

Results
There was no difference in the ossification level and shape of the skull between the experimental groups (control and two treatment concentrations) at stage 62. During the late larval period and metamorphosis, TH deficit had a significant impact on the level of bone ossification and skull shape with no differences between the two treatment concentrations of thiourea. The most pronounced differences in bone development were: the palatopterygoid failed to disintegrate into the palatal and pterygoid portions, retardation was observed in development of the maxilla, nasal and prefrontal bones and larval organization of the vomer was retained in thiourea-treated larvae.

Conclusions
This implies that deficiency of TH caused retardation in development and arrested metamorphic cranium skeletal reorganization, which resulted in divergent cranial shape compared to the control group. Our results confirmed that skull remodeling and ossification of late-appearing bones is TH–dependent, as in other studied Urodela species. Also, our results indicate that TH plays an important role in the establishment of skull shape during the ontogeny of Triturus newts, especially during the late larval period and metamorphosis, when TH concentrations reach their maximum.
PB  - London: PEERJ INC
T2  - PeerJ
T1  - Effects of thiourea on the skull of Triturus newts during ontogeny
VL  - 9
DO  - 10.7717/peerj.11535
SP  - e11535
ER  - 

@article{
author = "Ajduković, Maja and Vučić, Tijana and Cvijanović, Milena",
year = "2021",
abstract = "Background
In amphibians, thyroid hormone (TH) has a profound role in cranial development, especially in ossification of the late-appearing bones and remodeling of the skull. In the present study, we explored the influence of TH deficiency on bone ossification and resulting skull shape during the ontogeny of Triturus newt hybrid larvae obtained from interspecific crosses between T. ivanbureschi and T. macedonicus.

Methods
Larvae were treated with two concentrations of thiourea (an endocrine disruptor that chemically inhibits synthesis of TH) during the midlarval and late larval periods. Morphological differences of the cranium were assessed at the end of the midlarval period (ontogenetic stage 62) and the metamorphic stage after treatment during the late larval period.

Results
There was no difference in the ossification level and shape of the skull between the experimental groups (control and two treatment concentrations) at stage 62. During the late larval period and metamorphosis, TH deficit had a significant impact on the level of bone ossification and skull shape with no differences between the two treatment concentrations of thiourea. The most pronounced differences in bone development were: the palatopterygoid failed to disintegrate into the palatal and pterygoid portions, retardation was observed in development of the maxilla, nasal and prefrontal bones and larval organization of the vomer was retained in thiourea-treated larvae.

Conclusions
This implies that deficiency of TH caused retardation in development and arrested metamorphic cranium skeletal reorganization, which resulted in divergent cranial shape compared to the control group. Our results confirmed that skull remodeling and ossification of late-appearing bones is TH–dependent, as in other studied Urodela species. Also, our results indicate that TH plays an important role in the establishment of skull shape during the ontogeny of Triturus newts, especially during the late larval period and metamorphosis, when TH concentrations reach their maximum.",
publisher = "London: PEERJ INC",
journal = "PeerJ",
title = "Effects of thiourea on the skull of Triturus newts during ontogeny",
volume = "9",
doi = "10.7717/peerj.11535",
pages = "e11535"
}

Ajduković, M., Vučić, T.,& Cvijanović, M.. (2021). Effects of thiourea on the skull of Triturus newts during ontogeny. in PeerJ
London: PEERJ INC., 9, e11535.
https://doi.org/10.7717/peerj.11535

Ajduković M, Vučić T, Cvijanović M. Effects of thiourea on the skull of Triturus newts during ontogeny. in PeerJ. 2021;9:e11535.
doi:10.7717/peerj.11535 .

Ajduković, Maja, Vučić, Tijana, Cvijanović, Milena, "Effects of thiourea on the skull of Triturus newts during ontogeny" in PeerJ, 9 (2021):e11535,
https://doi.org/10.7717/peerj.11535 . .

TY  - JOUR
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Petrović, Tamara
AU  - Ajduković, Maja
AU  - Vučić, Tijana
AU  - Cvijanović, Milena
AU  - Faggio, Caterina
AU  - Prokić, Marko
PY  - 2021
UR  - https://www.mdpi.com/1660-4601/18/18/9653
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8464833
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4483
AB  - Thiourea is an established disruptor of thyroid hormone synthesis and is frequently used as an inhibitor of metamorphosis. The changes caused by thiourea can affect processes associated with the oxidative status of individuals (metabolic rate, the HPI axis, antioxidant system). We investigated the parameters of oxidative stress in crested newt (Triturus spp.) larvae during normal development in late larval stage 62 and newly metamorphosed individuals, and during thiourea-stimulated metamorphosis arrest in individuals exposed to low (0.05%) and high (0.1%) concentrations of thiourea. Both groups of crested newts exposed to thiourea retained their larval characteristics until the end of the experiment. The low activities of antioxidant enzymes and the high lipid peroxidation level pointed to increased oxidative stress in larvae at the beginning of stage 62 as compared to fully metamorphosed individuals. The activities of catalase (CAT) and glutathione-S-transferase (GST) and the concentration of sulfhydryl (SH) groups were significantly lower in larvae reared in aqueous solutions containing thiourea than in newly metamorphosed individuals. The high thiourea concentration (0.1%) affected the antioxidative parameters to the extent that oxidative damage could not be avoided, contrary to a lower concentration. Our results provide a first insight into the physiological adaptations of crested newts during normal development and simulated metamorphosis arrest.
PB  - Basel: MDPI
T2  - International Journal of Environmental Research and Public Health
T1  - Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis.
IS  - 18
VL  - 18
DO  - 10.3390/ijerph18189653
SP  - 9653
ER  - 

@article{
author = "Gavrić, Jelena and Despotović, Svetlana and Gavrilović, Branka and Radovanović, Tijana and Petrović, Tamara and Ajduković, Maja and Vučić, Tijana and Cvijanović, Milena and Faggio, Caterina and Prokić, Marko",
year = "2021",
abstract = "Thiourea is an established disruptor of thyroid hormone synthesis and is frequently used as an inhibitor of metamorphosis. The changes caused by thiourea can affect processes associated with the oxidative status of individuals (metabolic rate, the HPI axis, antioxidant system). We investigated the parameters of oxidative stress in crested newt (Triturus spp.) larvae during normal development in late larval stage 62 and newly metamorphosed individuals, and during thiourea-stimulated metamorphosis arrest in individuals exposed to low (0.05%) and high (0.1%) concentrations of thiourea. Both groups of crested newts exposed to thiourea retained their larval characteristics until the end of the experiment. The low activities of antioxidant enzymes and the high lipid peroxidation level pointed to increased oxidative stress in larvae at the beginning of stage 62 as compared to fully metamorphosed individuals. The activities of catalase (CAT) and glutathione-S-transferase (GST) and the concentration of sulfhydryl (SH) groups were significantly lower in larvae reared in aqueous solutions containing thiourea than in newly metamorphosed individuals. The high thiourea concentration (0.1%) affected the antioxidative parameters to the extent that oxidative damage could not be avoided, contrary to a lower concentration. Our results provide a first insight into the physiological adaptations of crested newts during normal development and simulated metamorphosis arrest.",
publisher = "Basel: MDPI",
journal = "International Journal of Environmental Research and Public Health",
title = "Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis.",
number = "18",
volume = "18",
doi = "10.3390/ijerph18189653",
pages = "9653"
}

Gavrić, J., Despotović, S., Gavrilović, B., Radovanović, T., Petrović, T., Ajduković, M., Vučić, T., Cvijanović, M., Faggio, C.,& Prokić, M.. (2021). Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis.. in International Journal of Environmental Research and Public Health
Basel: MDPI., 18(18), 9653.
https://doi.org/10.3390/ijerph18189653

Gavrić J, Despotović S, Gavrilović B, Radovanović T, Petrović T, Ajduković M, Vučić T, Cvijanović M, Faggio C, Prokić M. Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis.. in International Journal of Environmental Research and Public Health. 2021;18(18):9653.
doi:10.3390/ijerph18189653 .

Gavrić, Jelena, Despotović, Svetlana, Gavrilović, Branka, Radovanović, Tijana, Petrović, Tamara, Ajduković, Maja, Vučić, Tijana, Cvijanović, Milena, Faggio, Caterina, Prokić, Marko, "Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis." in International Journal of Environmental Research and Public Health, 18, no. 18 (2021):9653,
https://doi.org/10.3390/ijerph18189653 . .

TY  - JOUR
AU  - Scholtes, Stefan
AU  - Arntzen, Jan
AU  - Ajduković, Maja
AU  - Ivanović, Ana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4660
AB  - The salamander vertebral column is largely undifferentiated, with a series of more or less uniform rib bearing presacral vertebrae traditionally designated as the trunk region. We explored regionalisation of the salamander trunk in seven species and two subspecies of the salamander genus Lissotriton by the combination of micro computed tomography scanning and geometric morphometrics. The detailed information on trunk vertebral shape was subjected to a multidimensional cluster analysis and a phenotypic trajectory analysis. With these complementary approaches, we observed a clear morphological regionalization. Clustering analysis showed that the anterior trunk vertebrae (T1 and T2) have distinct morphologies that are shared by all taxa, whereas the subsequent, more posterior vertebrae show significant disparity between species. The phenotypic trajectory analysis revealed that all taxa share a common pattern and amount of shape change along the trunk region. Altogether, our results support the hypothesis of a conserved anterior-posterior developmental patterning which can be associated with different functional demands, reflecting (sub)species’ and possibly, regional ecological divergences within species.
PB  - Hoboken: Wiley
T2  - Journal of Anatomy
T1  - Variation in vertebrae shape across small-bodied newts reveals functional and developmental constraints acting upon the trunk region
DO  - 10.1111/joa.13591
ER  - 

@article{
author = "Scholtes, Stefan and Arntzen, Jan and Ajduković, Maja and Ivanović, Ana",
year = "2021",
abstract = "The salamander vertebral column is largely undifferentiated, with a series of more or less uniform rib bearing presacral vertebrae traditionally designated as the trunk region. We explored regionalisation of the salamander trunk in seven species and two subspecies of the salamander genus Lissotriton by the combination of micro computed tomography scanning and geometric morphometrics. The detailed information on trunk vertebral shape was subjected to a multidimensional cluster analysis and a phenotypic trajectory analysis. With these complementary approaches, we observed a clear morphological regionalization. Clustering analysis showed that the anterior trunk vertebrae (T1 and T2) have distinct morphologies that are shared by all taxa, whereas the subsequent, more posterior vertebrae show significant disparity between species. The phenotypic trajectory analysis revealed that all taxa share a common pattern and amount of shape change along the trunk region. Altogether, our results support the hypothesis of a conserved anterior-posterior developmental patterning which can be associated with different functional demands, reflecting (sub)species’ and possibly, regional ecological divergences within species.",
publisher = "Hoboken: Wiley",
journal = "Journal of Anatomy",
title = "Variation in vertebrae shape across small-bodied newts reveals functional and developmental constraints acting upon the trunk region",
doi = "10.1111/joa.13591"
}

Scholtes, S., Arntzen, J., Ajduković, M.,& Ivanović, A.. (2021). Variation in vertebrae shape across small-bodied newts reveals functional and developmental constraints acting upon the trunk region. in Journal of Anatomy
Hoboken: Wiley..
https://doi.org/10.1111/joa.13591

Scholtes S, Arntzen J, Ajduković M, Ivanović A. Variation in vertebrae shape across small-bodied newts reveals functional and developmental constraints acting upon the trunk region. in Journal of Anatomy. 2021;.
doi:10.1111/joa.13591 .

Scholtes, Stefan, Arntzen, Jan, Ajduković, Maja, Ivanović, Ana, "Variation in vertebrae shape across small-bodied newts reveals functional and developmental constraints acting upon the trunk region" in Journal of Anatomy (2021),
https://doi.org/10.1111/joa.13591 . .

TY  - DATA
AU  - Scholtes, Stefan
AU  - Willem, Jan
AU  - Ivanović, Ana
AU  - Ajduković, Maja
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4661
AB  - Raw landmark coordinates
Each trunk vertebrae (T1 to T13) per species obtained from landmarking 3D surface models of the specimens used in this study can be found in the zip file "Raw landmark coordinates.rar". Each file is labelled as follows: Vertebrae-"Species"-"Collection label"-"Vertebrae number". For example "Vertebrae-Ampelensis-OZ-62-G22595-T1". Collection labels can be found in Appendix table S1 and individual landmark descriptions in Appendix table S2.

Appendix table S1
Collection material analyzed and type of preservation: ethanol preserved and whole-mounts glycerine stored skeletons. Museum codes are: IBISS – University of Belgrade, Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, RMNH.RENA and ZMA.RENA – Naturalis Biodiversity Center, Leiden, The Netherlands. N - sample size.

Appendix table S2
Brief anatomical descriptions of 27 landmarks configured to Lissotriton trunk vertebrae.

Appendix table S3
Frequency (%) of morphotypes over Lissotriton taxon / population and vertebra number.

Appendix table S4
Results on the phenotypic trajectory analysis with P-values for pairwise statistical assessment of a) the amount of species-specific shape changes along trunk region, i.e., trajectory size, b) the general orientation of shape changes in multivariate shape space, i.e., trajectory direction and c) the pattern of shape changes of vertebrae along the trunk region, i.e., trajectory shape.

Supplementary figure S1

Geographical distribution of the (sub-)species of the genus Lissotriton after Arntzen et al., 2009, Ianella et al., 2017 and Wielstra et al.,2018. Taxa are coded by colours as shown in the legend. Numbered dots indicate the localities of the populations that were studied, as also listed in Supplementary table S1. Note that no material was available for L. italicus and L. lantzi.


Supplementary figure S2

Phenotypic trajectories of shape changes along the trunk region of the newt genus Lissotriton expressed over the first (PC1) and third principal component axis (PC3). Trajectories were calculated for nine (sub-)species. The three populations of the L. helveticus were analysed separately. Taxa are coded by colours as shown in the legend. Connected dots represent the mean vertebrae (T) shape from T1 to T12, as is highlighted for L. helveticus from Marcillé la Ville, France. Three-dimensional surface models visualize the corresponding shape changes.


Supplementary figure S2_3D.

Three-dimensional graph showing phenotypic trajectories of shape changes along the trunk region in nine (sub-)species of the newt genus Lissotriton. Open the file with a web browser such as Google Chrome or Mozilla Firefox to visualize the trajectories in three dimensional morphospace that as defined by the first, second and third principal component axis (PC1, PC2 and PC3).
PB  - Hoboken: Wiley
T2  - Journal of Anatomy
T1  - Supplementary material "Variation in vertebrae shape across small-bodied newts reveals functional and developmental constraints acting upon the trunk region"
DO  - 10.6084/m9.figshare.14254406
ER  - 

@misc{
author = "Scholtes, Stefan and Willem, Jan and Ivanović, Ana and Ajduković, Maja",
year = "2021",
abstract = "Raw landmark coordinates
Each trunk vertebrae (T1 to T13) per species obtained from landmarking 3D surface models of the specimens used in this study can be found in the zip file "Raw landmark coordinates.rar". Each file is labelled as follows: Vertebrae-"Species"-"Collection label"-"Vertebrae number". For example "Vertebrae-Ampelensis-OZ-62-G22595-T1". Collection labels can be found in Appendix table S1 and individual landmark descriptions in Appendix table S2.

Appendix table S1
Collection material analyzed and type of preservation: ethanol preserved and whole-mounts glycerine stored skeletons. Museum codes are: IBISS – University of Belgrade, Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, RMNH.RENA and ZMA.RENA – Naturalis Biodiversity Center, Leiden, The Netherlands. N - sample size.

Appendix table S2
Brief anatomical descriptions of 27 landmarks configured to Lissotriton trunk vertebrae.

Appendix table S3
Frequency (%) of morphotypes over Lissotriton taxon / population and vertebra number.

Appendix table S4
Results on the phenotypic trajectory analysis with P-values for pairwise statistical assessment of a) the amount of species-specific shape changes along trunk region, i.e., trajectory size, b) the general orientation of shape changes in multivariate shape space, i.e., trajectory direction and c) the pattern of shape changes of vertebrae along the trunk region, i.e., trajectory shape.

Supplementary figure S1

Geographical distribution of the (sub-)species of the genus Lissotriton after Arntzen et al., 2009, Ianella et al., 2017 and Wielstra et al.,2018. Taxa are coded by colours as shown in the legend. Numbered dots indicate the localities of the populations that were studied, as also listed in Supplementary table S1. Note that no material was available for L. italicus and L. lantzi.


Supplementary figure S2

Phenotypic trajectories of shape changes along the trunk region of the newt genus Lissotriton expressed over the first (PC1) and third principal component axis (PC3). Trajectories were calculated for nine (sub-)species. The three populations of the L. helveticus were analysed separately. Taxa are coded by colours as shown in the legend. Connected dots represent the mean vertebrae (T) shape from T1 to T12, as is highlighted for L. helveticus from Marcillé la Ville, France. Three-dimensional surface models visualize the corresponding shape changes.


Supplementary figure S2_3D.

Three-dimensional graph showing phenotypic trajectories of shape changes along the trunk region in nine (sub-)species of the newt genus Lissotriton. Open the file with a web browser such as Google Chrome or Mozilla Firefox to visualize the trajectories in three dimensional morphospace that as defined by the first, second and third principal component axis (PC1, PC2 and PC3).",
publisher = "Hoboken: Wiley",
journal = "Journal of Anatomy",
title = "Supplementary material "Variation in vertebrae shape across small-bodied newts reveals functional and developmental constraints acting upon the trunk region"",
doi = "10.6084/m9.figshare.14254406"
}

Scholtes, S., Willem, J., Ivanović, A.,& Ajduković, M.. (2021). Supplementary material "Variation in vertebrae shape across small-bodied newts reveals functional and developmental constraints acting upon the trunk region". in Journal of Anatomy
Hoboken: Wiley..
https://doi.org/10.6084/m9.figshare.14254406

Scholtes S, Willem J, Ivanović A, Ajduković M. Supplementary material "Variation in vertebrae shape across small-bodied newts reveals functional and developmental constraints acting upon the trunk region". in Journal of Anatomy. 2021;.
doi:10.6084/m9.figshare.14254406 .

Scholtes, Stefan, Willem, Jan, Ivanović, Ana, Ajduković, Maja, "Supplementary material "Variation in vertebrae shape across small-bodied newts reveals functional and developmental constraints acting upon the trunk region"" in Journal of Anatomy (2021),
https://doi.org/10.6084/m9.figshare.14254406 . .

TY  - JOUR
AU  - Urošević, Aleksandar
AU  - Ajduković, Maja
AU  - Arntzen, Jan W.
AU  - Ivanović, Ana
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3617
AB  - Serial homology or the repetition of equivalent developmental units and their derivatives is a phenomenon encountered in a variety of organisms, with the vertebrate axial skeleton as one of the most notable examples. Serially homologous structures can be viewed as an appropriate model system for studying morphological integration and modularity, due to the strong impact of development on their covariation. Here, we explored the pattern of morphological integration of the cranium and the first three serially homologous structures (atlas, first, and second trunk vertebrae) in salamandrid salamanders, using micro‐CT scanning and three‐dimensional geometric morphometrics. We explored the integration between structures at static and evolutionary levels. Effects of allometry on patterns of modularity were also taken into account. At the static level (within species), we analyzed inter‐individual variation in shape to detect functional modules and intra‐individual variation to detect developmental modules. Significant integration (based on inter‐individual variation) among all structures was detected and allometry is shown to be an important integrating factor. The pattern of intra‐individual, asymmetric variation indicates statistically significant developmental integration between the cranium and the atlas and between the first two trunk vertebrae. At the evolutionary level (among species), the cranium, atlas, and trunk vertebrae separate as different modules. Our results show that morphological integration at the evolutionary level coincides with morphological and functional differentiation of the axial skeleton, allowing the more or less independent evolutionary changes of the cranial skeleton and the vertebral column, regardless of the relatively strong integration at the static level. The observed patterns of morphological integration differ across levels, indicating different impacts of developmental and phylogenetic constraints and functional demands.
PB  - Wiley
T2  - Journal of Zoological Systematics and Evolutionary Research
T1  - Morphological integration and serial homology: A case study of the cranium and anterior vertebrae in salamanders
IS  - 4
VL  - 58
DO  - 10.1111/jzs.12374
SP  - 1206
EP  - 1219
ER  - 

@article{
author = "Urošević, Aleksandar and Ajduković, Maja and Arntzen, Jan W. and Ivanović, Ana",
year = "2020",
abstract = "Serial homology or the repetition of equivalent developmental units and their derivatives is a phenomenon encountered in a variety of organisms, with the vertebrate axial skeleton as one of the most notable examples. Serially homologous structures can be viewed as an appropriate model system for studying morphological integration and modularity, due to the strong impact of development on their covariation. Here, we explored the pattern of morphological integration of the cranium and the first three serially homologous structures (atlas, first, and second trunk vertebrae) in salamandrid salamanders, using micro‐CT scanning and three‐dimensional geometric morphometrics. We explored the integration between structures at static and evolutionary levels. Effects of allometry on patterns of modularity were also taken into account. At the static level (within species), we analyzed inter‐individual variation in shape to detect functional modules and intra‐individual variation to detect developmental modules. Significant integration (based on inter‐individual variation) among all structures was detected and allometry is shown to be an important integrating factor. The pattern of intra‐individual, asymmetric variation indicates statistically significant developmental integration between the cranium and the atlas and between the first two trunk vertebrae. At the evolutionary level (among species), the cranium, atlas, and trunk vertebrae separate as different modules. Our results show that morphological integration at the evolutionary level coincides with morphological and functional differentiation of the axial skeleton, allowing the more or less independent evolutionary changes of the cranial skeleton and the vertebral column, regardless of the relatively strong integration at the static level. The observed patterns of morphological integration differ across levels, indicating different impacts of developmental and phylogenetic constraints and functional demands.",
publisher = "Wiley",
journal = "Journal of Zoological Systematics and Evolutionary Research",
title = "Morphological integration and serial homology: A case study of the cranium and anterior vertebrae in salamanders",
number = "4",
volume = "58",
doi = "10.1111/jzs.12374",
pages = "1206-1219"
}

Urošević, A., Ajduković, M., Arntzen, J. W.,& Ivanović, A.. (2020). Morphological integration and serial homology: A case study of the cranium and anterior vertebrae in salamanders. in Journal of Zoological Systematics and Evolutionary Research
Wiley., 58(4), 1206-1219.
https://doi.org/10.1111/jzs.12374

Urošević A, Ajduković M, Arntzen JW, Ivanović A. Morphological integration and serial homology: A case study of the cranium and anterior vertebrae in salamanders. in Journal of Zoological Systematics and Evolutionary Research. 2020;58(4):1206-1219.
doi:10.1111/jzs.12374 .

Urošević, Aleksandar, Ajduković, Maja, Arntzen, Jan W., Ivanović, Ana, "Morphological integration and serial homology: A case study of the cranium and anterior vertebrae in salamanders" in Journal of Zoological Systematics and Evolutionary Research, 58, no. 4 (2020):1206-1219,
https://doi.org/10.1111/jzs.12374 . .

TY  - CONF
AU  - Urošević, Aleksandar
AU  - Ajduković, Maja
AU  - Arntzen, Jan W
AU  - Ivanović, Ana
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5528
AB  - Using micro-CT scanning and 3D geometric morphometrics of newt craniums and axial skeletons (firstthree vertebrae) we explored the pattern of morphological integration. We tested if i) directly connected seriallyhomologous structures are more integrated than separated ones and ii) morphological integration coincides withregional differentiation. We applied a multilevel approach by analyzing patterns of integration at static andevolutionary levels, i.e., within and between species respectively. At the static level we choose the genus Triturusas a representative monophyletic group. We analysed between-individual variation in shape to detect functionalmodules and within-individuals the asymmetric component of variation in shape to detect developmental modules.At the evolutionary level, 17 species from five genera were analysed in phylogenetic context and taking effects ofallometry on modularity and integration into account. We found that allometry is an important integrating factorin serially homologous structures. At the static level and after the correction for allometry, functional integrationbetween the cranium and first vertebrae was weak but statistically significant between all elements, anddevelopmental integration was significant between the cranium and the atlas and first and second trunk vertebrae.At the evolutionary level, the cranium, atlas and trunk vertebrae separate as three different modules. Our resultssuggest that, at the evolutionary level, morphological integration coincide with regional and functionaldifferentiation of the axial skeleton. This allows the relatively independent evolution of the cranial skeleton andthe vertebral column, separate of the significant functional and developmental integration at the static level.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković" - National Institute of the Republic of Serbia, University of Belgrade
C3  - XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy
T1  - Мorphological integration of the cranium and axial skeleton in european newts
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5528
ER  - 

@conference{
author = "Urošević, Aleksandar and Ajduković, Maja and Arntzen, Jan W and Ivanović, Ana",
year = "2019",
abstract = "Using micro-CT scanning and 3D geometric morphometrics of newt craniums and axial skeletons (firstthree vertebrae) we explored the pattern of morphological integration. We tested if i) directly connected seriallyhomologous structures are more integrated than separated ones and ii) morphological integration coincides withregional differentiation. We applied a multilevel approach by analyzing patterns of integration at static andevolutionary levels, i.e., within and between species respectively. At the static level we choose the genus Triturusas a representative monophyletic group. We analysed between-individual variation in shape to detect functionalmodules and within-individuals the asymmetric component of variation in shape to detect developmental modules.At the evolutionary level, 17 species from five genera were analysed in phylogenetic context and taking effects ofallometry on modularity and integration into account. We found that allometry is an important integrating factorin serially homologous structures. At the static level and after the correction for allometry, functional integrationbetween the cranium and first vertebrae was weak but statistically significant between all elements, anddevelopmental integration was significant between the cranium and the atlas and first and second trunk vertebrae.At the evolutionary level, the cranium, atlas and trunk vertebrae separate as three different modules. Our resultssuggest that, at the evolutionary level, morphological integration coincide with regional and functionaldifferentiation of the axial skeleton. This allows the relatively independent evolution of the cranial skeleton andthe vertebral column, separate of the significant functional and developmental integration at the static level.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković" - National Institute of the Republic of Serbia, University of Belgrade",
journal = "XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy",
title = "Мorphological integration of the cranium and axial skeleton in european newts",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5528"
}

Urošević, A., Ajduković, M., Arntzen, J. W.,& Ivanović, A.. (2019). Мorphological integration of the cranium and axial skeleton in european newts. in XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy
Belgrade: Institute for Biological Research "Siniša Stanković" - National Institute of the Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_5528

Urošević A, Ajduković M, Arntzen JW, Ivanović A. Мorphological integration of the cranium and axial skeleton in european newts. in XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy. 2019;.
https://hdl.handle.net/21.15107/rcub_ibiss_5528 .

Urošević, Aleksandar, Ajduković, Maja, Arntzen, Jan W, Ivanović, Ana, "Мorphological integration of the cranium and axial skeleton in european newts" in XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy (2019),
https://hdl.handle.net/21.15107/rcub_ibiss_5528 .

TY  - CONF
AU  - Urošević, Aleksandar
AU  - Ajduković, Maja
AU  - Arntzen, Jan W
AU  - Ivanović, Ana
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5527
AB  - Using micro-CT scanning and 3D geometric morphometrics of newt craniums and axial skeletons (first
three vertebrae) we explored the pattern of morphological integration. We tested if i) directly connected serially
homologous structures are more integrated than separated ones and ii) morphological integration coincides with
regional differentiation. We applied a multilevel approach by analyzing patterns of integration at static and
evolutionary levels, i.e., within and between species respectively. At the static level we choose the genus Triturus
as a representative monophyletic group. We analysed between-individual variation in shape to detect functional
modules and within-individuals the asymmetric component of variation in shape to detect developmental modules.
At the evolutionary level, 17 species from five genera were analysed in phylogenetic context and taking effects of
allometry on modularity and integration into account. We found that allometry is an important integrating factor
in serially homologous structures. At the static level and after the correction for allometry, functional integration
between the cranium and first vertebrae was weak but statistically significant between all elements, and
developmental integration was significant between the cranium and the atlas and first and second trunk vertebrae.
At the evolutionary level, the cranium, atlas and trunk vertebrae separate as three different modules. Our results
suggest that, at the evolutionary level, morphological integration coincide with regional and functional
differentiation of the axial skeleton. This allows the relatively independent evolution of the cranial skeleton and
the vertebral column, separate of the significant functional and developmental integration at the static level.
PB  - Milan, Italy: University of Milan, the University of Pavia, the Natural History Museum of Milan and the University of Milano-Bicocca
C3  - XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy
T1  - Мorphological integration of the cranium and axial skeleton in european newts
SP  - 142
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5527
ER  - 

@conference{
author = "Urošević, Aleksandar and Ajduković, Maja and Arntzen, Jan W and Ivanović, Ana",
year = "2019",
abstract = "Using micro-CT scanning and 3D geometric morphometrics of newt craniums and axial skeletons (first
three vertebrae) we explored the pattern of morphological integration. We tested if i) directly connected serially
homologous structures are more integrated than separated ones and ii) morphological integration coincides with
regional differentiation. We applied a multilevel approach by analyzing patterns of integration at static and
evolutionary levels, i.e., within and between species respectively. At the static level we choose the genus Triturus
as a representative monophyletic group. We analysed between-individual variation in shape to detect functional
modules and within-individuals the asymmetric component of variation in shape to detect developmental modules.
At the evolutionary level, 17 species from five genera were analysed in phylogenetic context and taking effects of
allometry on modularity and integration into account. We found that allometry is an important integrating factor
in serially homologous structures. At the static level and after the correction for allometry, functional integration
between the cranium and first vertebrae was weak but statistically significant between all elements, and
developmental integration was significant between the cranium and the atlas and first and second trunk vertebrae.
At the evolutionary level, the cranium, atlas and trunk vertebrae separate as three different modules. Our results
suggest that, at the evolutionary level, morphological integration coincide with regional and functional
differentiation of the axial skeleton. This allows the relatively independent evolution of the cranial skeleton and
the vertebral column, separate of the significant functional and developmental integration at the static level.",
publisher = "Milan, Italy: University of Milan, the University of Pavia, the Natural History Museum of Milan and the University of Milano-Bicocca",
journal = "XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy",
title = "Мorphological integration of the cranium and axial skeleton in european newts",
pages = "142",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5527"
}

Urošević, A., Ajduković, M., Arntzen, J. W.,& Ivanović, A.. (2019). Мorphological integration of the cranium and axial skeleton in european newts. in XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy
Milan, Italy: University of Milan, the University of Pavia, the Natural History Museum of Milan and the University of Milano-Bicocca., 142.
https://hdl.handle.net/21.15107/rcub_ibiss_5527

Urošević A, Ajduković M, Arntzen JW, Ivanović A. Мorphological integration of the cranium and axial skeleton in european newts. in XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy. 2019;:142.
https://hdl.handle.net/21.15107/rcub_ibiss_5527 .

Urošević, Aleksandar, Ajduković, Maja, Arntzen, Jan W, Ivanović, Ana, "Мorphological integration of the cranium and axial skeleton in european newts" in XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy (2019):142,
https://hdl.handle.net/21.15107/rcub_ibiss_5527 .

TY  - CONF
AU  - Anđelković, Marko
AU  - Neda, Bogdanović
AU  - Denon, Gorana
AU  - Mirč, Marko
AU  - Vučić, Tijana
AU  - Ajduković, Maja
AU  - Vukov, Tanja
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3498
AB  - Millions of animals are roadkilled every year. Amphibians and reptiles are among the most prone to roadkill, especially near ponds and wetlands. We examined the impact of traffic on amphibian and reptile populations on the edge of Special nature reserve “Obedska bara”, Serbia. The aim of our study was to examine the temporal and spatial patterns of amphibian and reptile road mortality. From March to December 2018, we surveyed the road on foot for roadkills on a road section of 4.2 km, with two different habitat types (forest-pond and agricultural area-pond). Within 32 fieldwork days, we recorded 20457 roadkills (nine amphibian and eight reptile species). Amphibians (93%) were more vulnerable to traffic than reptiles (7%). Specimens of Pelophylax esculentus complex are the most frequently killed amphibians, followed by Pelobates fuscus, while specimens of Natrix natrix were the most frequently killed reptile species, followed by Emys orbicularis. The numbers of killed amphibians and reptiles varied with the season, indicating that species do not have the same roadkill patterns among the different seasons. Amphibian roadkills were the most frequent during the summer, while reptile roadkills were the most frequent in spring and late summer. Habitat type significantly affected the vulnerability of animals on roads. In addition to the different number of individuals killed in two different habitat types (higher in forest habitat), there was a difference in species composition. This study indicates that detailed monitoring and actions that will mitigate road mortality are urgently needed in the Special nature reserve “Obedska bara“.
C3  - XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy, 2019
T1  - Amphibian and reptile road mortality in the protected area of Obedska Bara, Serbia
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3498
ER  - 

@conference{
author = "Anđelković, Marko and Neda, Bogdanović and Denon, Gorana and Mirč, Marko and Vučić, Tijana and Ajduković, Maja and Vukov, Tanja",
year = "2019",
abstract = "Millions of animals are roadkilled every year. Amphibians and reptiles are among the most prone to roadkill, especially near ponds and wetlands. We examined the impact of traffic on amphibian and reptile populations on the edge of Special nature reserve “Obedska bara”, Serbia. The aim of our study was to examine the temporal and spatial patterns of amphibian and reptile road mortality. From March to December 2018, we surveyed the road on foot for roadkills on a road section of 4.2 km, with two different habitat types (forest-pond and agricultural area-pond). Within 32 fieldwork days, we recorded 20457 roadkills (nine amphibian and eight reptile species). Amphibians (93%) were more vulnerable to traffic than reptiles (7%). Specimens of Pelophylax esculentus complex are the most frequently killed amphibians, followed by Pelobates fuscus, while specimens of Natrix natrix were the most frequently killed reptile species, followed by Emys orbicularis. The numbers of killed amphibians and reptiles varied with the season, indicating that species do not have the same roadkill patterns among the different seasons. Amphibian roadkills were the most frequent during the summer, while reptile roadkills were the most frequent in spring and late summer. Habitat type significantly affected the vulnerability of animals on roads. In addition to the different number of individuals killed in two different habitat types (higher in forest habitat), there was a difference in species composition. This study indicates that detailed monitoring and actions that will mitigate road mortality are urgently needed in the Special nature reserve “Obedska bara“.",
journal = "XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy, 2019",
title = "Amphibian and reptile road mortality in the protected area of Obedska Bara, Serbia",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3498"
}

Anđelković, M., Neda, B., Denon, G., Mirč, M., Vučić, T., Ajduković, M.,& Vukov, T.. (2019). Amphibian and reptile road mortality in the protected area of Obedska Bara, Serbia. in XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy, 2019.
https://hdl.handle.net/21.15107/rcub_ibiss_3498

Anđelković M, Neda B, Denon G, Mirč M, Vučić T, Ajduković M, Vukov T. Amphibian and reptile road mortality in the protected area of Obedska Bara, Serbia. in XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy, 2019. 2019;.
https://hdl.handle.net/21.15107/rcub_ibiss_3498 .

Anđelković, Marko, Neda, Bogdanović, Denon, Gorana, Mirč, Marko, Vučić, Tijana, Ajduković, Maja, Vukov, Tanja, "Amphibian and reptile road mortality in the protected area of Obedska Bara, Serbia" in XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy, 2019 (2019),
https://hdl.handle.net/21.15107/rcub_ibiss_3498 .

TY  - CONF
AU  - Urošević, Aleksandar
AU  - Ajduković, Maja
AU  - Jan Willem, Arntzen
AU  - Ivanović, Ana
PY  - 2019
UR  - http://seh-congress-2019.unipv.it/wp-content/uploads/2019/08/SEHXX-abstract-book-3.pdf
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3496
AB  - Using micro-CT scanning and 3D geometric morphometrics of newt craniums and axial skeletons (first three vertebrae) we explored the pattern of morphological integration. We tested if i) directly connected serially homologous structures are more integrated than separated ones and ii) morphological integration coincides with regional differentiation. We applied a multilevel approach by analyzing patterns of integration at static and evolutionary levels, i.e., within and between species respectively. At the static level we choose the genus Triturus as a representative monophyletic group. We analysed between-individual variation in shape to detect functional modules and within-individuals the asymmetric component of variation in shape to detect developmental modules. At the evolutionary level, 17 species from five genera were analysed in phylogenetic context and taking effects of allometry on modularity and integration into account. We found that allometry is an important integrating factor in serially homologous structures. At the static level and after the correction for allometry, functional integration between the cranium and first vertebrae was weak but statistically significant between all elements, and developmental integration was significant between the cranium and the atlas and first and second trunk vertebrae. At the evolutionary level, the cranium, atlas and trunk vertebrae separate as three different modules. Our results suggest that, at the evolutionary level, morphological integration coincide with regional and functional differentiation of the axial skeleton. This allows the relatively independent evolution of the cranial skeleton and the vertebral column, separate of the significant functional and developmental integration at the static level.
PB  - Milan, Italy: University of Milan, the University of Pavia, the Natural History Museum of Milan and the University of Milano-Bicocca
C3  - XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy
T1  - Morphological integration of the cranium and axial skeleton In European newts
SP  - 142
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3496
ER  - 

@conference{
author = "Urošević, Aleksandar and Ajduković, Maja and Jan Willem, Arntzen and Ivanović, Ana",
year = "2019",
abstract = "Using micro-CT scanning and 3D geometric morphometrics of newt craniums and axial skeletons (first three vertebrae) we explored the pattern of morphological integration. We tested if i) directly connected serially homologous structures are more integrated than separated ones and ii) morphological integration coincides with regional differentiation. We applied a multilevel approach by analyzing patterns of integration at static and evolutionary levels, i.e., within and between species respectively. At the static level we choose the genus Triturus as a representative monophyletic group. We analysed between-individual variation in shape to detect functional modules and within-individuals the asymmetric component of variation in shape to detect developmental modules. At the evolutionary level, 17 species from five genera were analysed in phylogenetic context and taking effects of allometry on modularity and integration into account. We found that allometry is an important integrating factor in serially homologous structures. At the static level and after the correction for allometry, functional integration between the cranium and first vertebrae was weak but statistically significant between all elements, and developmental integration was significant between the cranium and the atlas and first and second trunk vertebrae. At the evolutionary level, the cranium, atlas and trunk vertebrae separate as three different modules. Our results suggest that, at the evolutionary level, morphological integration coincide with regional and functional differentiation of the axial skeleton. This allows the relatively independent evolution of the cranial skeleton and the vertebral column, separate of the significant functional and developmental integration at the static level.",
publisher = "Milan, Italy: University of Milan, the University of Pavia, the Natural History Museum of Milan and the University of Milano-Bicocca",
journal = "XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy",
title = "Morphological integration of the cranium and axial skeleton In European newts",
pages = "142",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3496"
}

Urošević, A., Ajduković, M., Jan Willem, A.,& Ivanović, A.. (2019). Morphological integration of the cranium and axial skeleton In European newts. in XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy
Milan, Italy: University of Milan, the University of Pavia, the Natural History Museum of Milan and the University of Milano-Bicocca., 142.
https://hdl.handle.net/21.15107/rcub_ibiss_3496

Urošević A, Ajduković M, Jan Willem A, Ivanović A. Morphological integration of the cranium and axial skeleton In European newts. in XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy. 2019;:142.
https://hdl.handle.net/21.15107/rcub_ibiss_3496 .

Urošević, Aleksandar, Ajduković, Maja, Jan Willem, Arntzen, Ivanović, Ana, "Morphological integration of the cranium and axial skeleton In European newts" in XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy (2019):142,
https://hdl.handle.net/21.15107/rcub_ibiss_3496 .

TY  - JOUR
AU  - Arntzen, Jan W.
AU  - Üzüm, Nazan
AU  - Ajduković, Maja
AU  - Ivanović, Ana
AU  - Wielstra, Ben
PY  - 2018
UR  - https://peerj.com/articles/5317
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3120
AB  - Relationships between phylogenetic relatedness, hybrid zone spatial structure, the amount of interspecific gene flow and population demography were investigated, with the newt genus Triturus as a model system. In earlier work, a bimodal hybrid zone of two distantly related species combined low interspecific gene flow with hybrid sterility and heterosis was documented. Apart from that, a suite of unimodal hybrid zones in closely related Triturus showed more or less extensive introgressive hybridization with no evidence for heterosis. We here report on population demography and interspecific gene flow in two Triturus species ( T. macedonicus and T. ivanbureschi in Serbia). These are two that are moderately related, engage in a heterogeneous uni-/bimodal hybrid zone and hence represent an intermediate situation. This study used 13 diagnostic nuclear genetic markers in a population at the species contact zone. This showed that all individuals were hybrids, with no parentals detected. Age, size and longevity and the estimated growth curves are not exceeding that of the parental species, so that we conclude the absence of heterosis in T. macedonicus – T. ivanbureschi . Observations across the genus support the hypothesis that fertile hybrids allocate resources to reproduction and infertile hybrids allocate resources to growth. Several Triturus species hybrid zones not yet studied allow the testing of this hypothesis.
T2  - PeerJ
T1  - Absence of heterosis in hybrid crested newts
VL  - 6
DO  - 10.7717/peerj.5317
SP  - e5317
ER  - 

@article{
author = "Arntzen, Jan W. and Üzüm, Nazan and Ajduković, Maja and Ivanović, Ana and Wielstra, Ben",
year = "2018",
abstract = "Relationships between phylogenetic relatedness, hybrid zone spatial structure, the amount of interspecific gene flow and population demography were investigated, with the newt genus Triturus as a model system. In earlier work, a bimodal hybrid zone of two distantly related species combined low interspecific gene flow with hybrid sterility and heterosis was documented. Apart from that, a suite of unimodal hybrid zones in closely related Triturus showed more or less extensive introgressive hybridization with no evidence for heterosis. We here report on population demography and interspecific gene flow in two Triturus species ( T. macedonicus and T. ivanbureschi in Serbia). These are two that are moderately related, engage in a heterogeneous uni-/bimodal hybrid zone and hence represent an intermediate situation. This study used 13 diagnostic nuclear genetic markers in a population at the species contact zone. This showed that all individuals were hybrids, with no parentals detected. Age, size and longevity and the estimated growth curves are not exceeding that of the parental species, so that we conclude the absence of heterosis in T. macedonicus – T. ivanbureschi . Observations across the genus support the hypothesis that fertile hybrids allocate resources to reproduction and infertile hybrids allocate resources to growth. Several Triturus species hybrid zones not yet studied allow the testing of this hypothesis.",
journal = "PeerJ",
title = "Absence of heterosis in hybrid crested newts",
volume = "6",
doi = "10.7717/peerj.5317",
pages = "e5317"
}

Arntzen, J. W., Üzüm, N., Ajduković, M., Ivanović, A.,& Wielstra, B.. (2018). Absence of heterosis in hybrid crested newts. in PeerJ, 6, e5317.
https://doi.org/10.7717/peerj.5317

Arntzen JW, Üzüm N, Ajduković M, Ivanović A, Wielstra B. Absence of heterosis in hybrid crested newts. in PeerJ. 2018;6:e5317.
doi:10.7717/peerj.5317 .

Arntzen, Jan W., Üzüm, Nazan, Ajduković, Maja, Ivanović, Ana, Wielstra, Ben, "Absence of heterosis in hybrid crested newts" in PeerJ, 6 (2018):e5317,
https://doi.org/10.7717/peerj.5317 . .

TY  - JOUR
AU  - Urošević, Aleksandar
AU  - Tomović, Ljiljana
AU  - Krizmanić, Imre
AU  - Anđelković, Marko
AU  - Golubović, Ana
AU  - Maričić, Marko
AU  - Ajtić, Rastko
AU  - Ćorović, Jelena
AU  - Čubrić, Tijana
AU  - Tomašević-Kolarov, Nataša
AU  - Cvijanović, Milena
AU  - Vukov, Tanja
AU  - Jovanović, Bogdan
AU  - Vučić, Tijana
AU  - Ajduković, Maja
AU  - Tot, Ivan
AU  - Nadaždin, Bojana
AU  - Labus, Nenad
AU  - Džukić, Georg
PY  - 2018
UR  - http://www.nhmbeo.rs/издања/bulletin.65.html
UR  - http://www.nhmbeo.rs/upload/images/Glasnik/2018_a/Bulletin 2018-09.pdf
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3292
AB  - In this study, we present updated distribution data for all three brown frog species (Rana spp.) inhabiting Serbia. The data provided consists of newly collected field records and compiled data previously published in literature or via Internet. Of the three species found in Serbia, Rana dalmatina is the most widespread, present in all three altitudinal regions and all biogeographical regions. Rana graeca is confined to Mountain-valley altitudinal region in permanent fastflowing rivers or streams and it reaches its northern range boundaries in Serbia. Rana temporaria is the rarest of the three, with fragmented range restricted to the high mountains or few isolated lowland populations, and in the north-eastern Serbia, it is vicariant with R. graeca in canyons and gorges of montane rivers. The presence of Rana arvalis in Serbia is mentioned in literature but was not confirmed during the subsequent field surveys, so we consider it only as a potential species for the Serbian batrachofauna. The brown frog faunal composition of Serbia is identical to that of Albania, Bosnia and Herzegovina, Bulgaria, Greece, Macedonia and Montenegro, while it is somewhat different from that of Hungary and Romania and the most different from Croatia and Slovenia. Serbian brown frogs belong to European (R. temporaria) and South-European (R. dalmatina, R. graeca) chorotypes. All species of brown frogs present or potentially present in Serbia and their habitats are identified as great conservation priorities.
T2  - Bulletin of the Natural History Museum
T1  - Distribution and diversity of brown frogs (Rana spp., Anura, Amphibia) in Serbia
VL  - 11
DO  - 10.5937/bnhmb1306090V
SP  - 227
EP  - 245
ER  - 

@article{
author = "Urošević, Aleksandar and Tomović, Ljiljana and Krizmanić, Imre and Anđelković, Marko and Golubović, Ana and Maričić, Marko and Ajtić, Rastko and Ćorović, Jelena and Čubrić, Tijana and Tomašević-Kolarov, Nataša and Cvijanović, Milena and Vukov, Tanja and Jovanović, Bogdan and Vučić, Tijana and Ajduković, Maja and Tot, Ivan and Nadaždin, Bojana and Labus, Nenad and Džukić, Georg",
year = "2018",
abstract = "In this study, we present updated distribution data for all three brown frog species (Rana spp.) inhabiting Serbia. The data provided consists of newly collected field records and compiled data previously published in literature or via Internet. Of the three species found in Serbia, Rana dalmatina is the most widespread, present in all three altitudinal regions and all biogeographical regions. Rana graeca is confined to Mountain-valley altitudinal region in permanent fastflowing rivers or streams and it reaches its northern range boundaries in Serbia. Rana temporaria is the rarest of the three, with fragmented range restricted to the high mountains or few isolated lowland populations, and in the north-eastern Serbia, it is vicariant with R. graeca in canyons and gorges of montane rivers. The presence of Rana arvalis in Serbia is mentioned in literature but was not confirmed during the subsequent field surveys, so we consider it only as a potential species for the Serbian batrachofauna. The brown frog faunal composition of Serbia is identical to that of Albania, Bosnia and Herzegovina, Bulgaria, Greece, Macedonia and Montenegro, while it is somewhat different from that of Hungary and Romania and the most different from Croatia and Slovenia. Serbian brown frogs belong to European (R. temporaria) and South-European (R. dalmatina, R. graeca) chorotypes. All species of brown frogs present or potentially present in Serbia and their habitats are identified as great conservation priorities.",
journal = "Bulletin of the Natural History Museum",
title = "Distribution and diversity of brown frogs (Rana spp., Anura, Amphibia) in Serbia",
volume = "11",
doi = "10.5937/bnhmb1306090V",
pages = "227-245"
}

Urošević, A., Tomović, L., Krizmanić, I., Anđelković, M., Golubović, A., Maričić, M., Ajtić, R., Ćorović, J., Čubrić, T., Tomašević-Kolarov, N., Cvijanović, M., Vukov, T., Jovanović, B., Vučić, T., Ajduković, M., Tot, I., Nadaždin, B., Labus, N.,& Džukić, G.. (2018). Distribution and diversity of brown frogs (Rana spp., Anura, Amphibia) in Serbia. in Bulletin of the Natural History Museum, 11, 227-245.
https://doi.org/10.5937/bnhmb1306090V

Urošević A, Tomović L, Krizmanić I, Anđelković M, Golubović A, Maričić M, Ajtić R, Ćorović J, Čubrić T, Tomašević-Kolarov N, Cvijanović M, Vukov T, Jovanović B, Vučić T, Ajduković M, Tot I, Nadaždin B, Labus N, Džukić G. Distribution and diversity of brown frogs (Rana spp., Anura, Amphibia) in Serbia. in Bulletin of the Natural History Museum. 2018;11:227-245.
doi:10.5937/bnhmb1306090V .

Urošević, Aleksandar, Tomović, Ljiljana, Krizmanić, Imre, Anđelković, Marko, Golubović, Ana, Maričić, Marko, Ajtić, Rastko, Ćorović, Jelena, Čubrić, Tijana, Tomašević-Kolarov, Nataša, Cvijanović, Milena, Vukov, Tanja, Jovanović, Bogdan, Vučić, Tijana, Ajduković, Maja, Tot, Ivan, Nadaždin, Bojana, Labus, Nenad, Džukić, Georg, "Distribution and diversity of brown frogs (Rana spp., Anura, Amphibia) in Serbia" in Bulletin of the Natural History Museum, 11 (2018):227-245,
https://doi.org/10.5937/bnhmb1306090V . .