TY  - JOUR
AU  - Ajduković, Maja
AU  - Ukropina, Mirela
AU  - Cvijanović, Milena
AU  - Vučić, Tijana
AU  - Ivanović, Ana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5579
AB  - Background: Amphibian skin has been studied for many decades, especially the metamorphic changes in
the skin of frogs. Less attention has been paid to salamander skin. Here, we describe changes in the skin
structure during postembryonic development in a salamandrid species, the Balkan crested newt Triturus
ivanbureschi.
Method: Using traditional histological techniques we examined the skin in the trunk region of three premetamorphic
larval stages (hatchling, mid larval and late larval) and two postmetamorphic stages (juvenile,
just after metamorphosis, and adult).
Results: In larval stages, skin consists only of the epidermis, which gradually develops from the single
epithelial cell layer in hatchlings, to a stratified epidermis with gland nests and characteristic Leydig cells at
the late larval stage. During metamorphosis, Leydig cells disappear, and the dermal layer develops. In
postmetamorphic stages, skin is differentiated on stratified epidermis and the dermis with well-developed
glands. Three types of glands were observed in the skin of the postmetamorphic stages: mucous, granular
and mixed. Gland composition appears to be stage- and sex-specific, with juveniles and adult female being
more similar to each other. In juveniles and adult female, there are a similar proportion of glands in both
dorsal and ventral skin, whereas in adult male granular glands dominated the dorsal skin, while mixed
glands dominated the ventral skin.
Conclusion: Our results provide a baseline for future comparative research of skin anatomy in salamanders.
PB  - Elsevier GmbH.
T2  - Annals of Anatomy - Anatomischer Anzeiger
T1  - Histological changes of the skin during postembryonic development of the crested newt Triturus ivanbureschi (Urodela, Salamandridae)
VL  - 249
DO  - 10.1016/j.aanat.2023.152097
SP  - 152097
ER  - 

@article{
author = "Ajduković, Maja and Ukropina, Mirela and Cvijanović, Milena and Vučić, Tijana and Ivanović, Ana",
year = "2023",
abstract = "Background: Amphibian skin has been studied for many decades, especially the metamorphic changes in
the skin of frogs. Less attention has been paid to salamander skin. Here, we describe changes in the skin
structure during postembryonic development in a salamandrid species, the Balkan crested newt Triturus
ivanbureschi.
Method: Using traditional histological techniques we examined the skin in the trunk region of three premetamorphic
larval stages (hatchling, mid larval and late larval) and two postmetamorphic stages (juvenile,
just after metamorphosis, and adult).
Results: In larval stages, skin consists only of the epidermis, which gradually develops from the single
epithelial cell layer in hatchlings, to a stratified epidermis with gland nests and characteristic Leydig cells at
the late larval stage. During metamorphosis, Leydig cells disappear, and the dermal layer develops. In
postmetamorphic stages, skin is differentiated on stratified epidermis and the dermis with well-developed
glands. Three types of glands were observed in the skin of the postmetamorphic stages: mucous, granular
and mixed. Gland composition appears to be stage- and sex-specific, with juveniles and adult female being
more similar to each other. In juveniles and adult female, there are a similar proportion of glands in both
dorsal and ventral skin, whereas in adult male granular glands dominated the dorsal skin, while mixed
glands dominated the ventral skin.
Conclusion: Our results provide a baseline for future comparative research of skin anatomy in salamanders.",
publisher = "Elsevier GmbH.",
journal = "Annals of Anatomy - Anatomischer Anzeiger",
title = "Histological changes of the skin during postembryonic development of the crested newt Triturus ivanbureschi (Urodela, Salamandridae)",
volume = "249",
doi = "10.1016/j.aanat.2023.152097",
pages = "152097"
}

Ajduković, M., Ukropina, M., Cvijanović, M., Vučić, T.,& Ivanović, A.. (2023). Histological changes of the skin during postembryonic development of the crested newt Triturus ivanbureschi (Urodela, Salamandridae). in Annals of Anatomy - Anatomischer Anzeiger
Elsevier GmbH.., 249, 152097.
https://doi.org/10.1016/j.aanat.2023.152097

Ajduković M, Ukropina M, Cvijanović M, Vučić T, Ivanović A. Histological changes of the skin during postembryonic development of the crested newt Triturus ivanbureschi (Urodela, Salamandridae). in Annals of Anatomy - Anatomischer Anzeiger. 2023;249:152097.
doi:10.1016/j.aanat.2023.152097 .

Ajduković, Maja, Ukropina, Mirela, Cvijanović, Milena, Vučić, Tijana, Ivanović, Ana, "Histological changes of the skin during postembryonic development of the crested newt Triturus ivanbureschi (Urodela, Salamandridae)" in Annals of Anatomy - Anatomischer Anzeiger, 249 (2023):152097,
https://doi.org/10.1016/j.aanat.2023.152097 . .

TY  - JOUR
AU  - Urošević, Aleksandar
AU  - Maričić, Marko
AU  - Šević, Mirko
AU  - Vučić, Tijana
AU  - Mirč, Marko
AU  - Tomović, Ljiljana
AU  - Anđelković, Marko
PY  - 2023
UR  - https://www.biotaxa.org/hn/article/view/80732
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5923
PB  - Societa Europea Herpetologica
T2  - Herpetology Notes
T1  - Note on the further spread of the Kotschy’s Gecko (Mediodactylus kotschyi) in Serbia with pholidosis description
VL  - 16
SP  - 533
EP  - 537
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5923
ER  - 

@article{
author = "Urošević, Aleksandar and Maričić, Marko and Šević, Mirko and Vučić, Tijana and Mirč, Marko and Tomović, Ljiljana and Anđelković, Marko",
year = "2023",
publisher = "Societa Europea Herpetologica",
journal = "Herpetology Notes",
title = "Note on the further spread of the Kotschy’s Gecko (Mediodactylus kotschyi) in Serbia with pholidosis description",
volume = "16",
pages = "533-537",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5923"
}

Urošević, A., Maričić, M., Šević, M., Vučić, T., Mirč, M., Tomović, L.,& Anđelković, M.. (2023). Note on the further spread of the Kotschy’s Gecko (Mediodactylus kotschyi) in Serbia with pholidosis description. in Herpetology Notes
Societa Europea Herpetologica., 16, 533-537.
https://hdl.handle.net/21.15107/rcub_ibiss_5923

Urošević A, Maričić M, Šević M, Vučić T, Mirč M, Tomović L, Anđelković M. Note on the further spread of the Kotschy’s Gecko (Mediodactylus kotschyi) in Serbia with pholidosis description. in Herpetology Notes. 2023;16:533-537.
https://hdl.handle.net/21.15107/rcub_ibiss_5923 .

Urošević, Aleksandar, Maričić, Marko, Šević, Mirko, Vučić, Tijana, Mirč, Marko, Tomović, Ljiljana, Anđelković, Marko, "Note on the further spread of the Kotschy’s Gecko (Mediodactylus kotschyi) in Serbia with pholidosis description" in Herpetology Notes, 16 (2023):533-537,
https://hdl.handle.net/21.15107/rcub_ibiss_5923 .

TY  - JOUR
AU  - Urošević, Aleksandar
AU  - Ajduković, Maja
AU  - Vučić, Tijana
AU  - Scholtes, Stefan
AU  - Arntzen, Jan, W
AU  - Ivanović, Ana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5817
AB  - Serially homologous structures may have complex patterns of regionalization and
morphological integration, influenced by developmental Hox gene expression and
functional constraints. The vertebral column, consisting of a number of repeated,
developmentally constrained, and highly integrated units—vertebrae—is such a
complex serially homologous structure. Functional diversification increases regionalization
and modularity of the vertebral column, particularly in mammals. For
salamanders, three concepts of regionalization of the vertebral column have been
proposed, recognizing one, two, or three presacral regions. Using three‐dimensional
geometric morphometrics on vertebra models acquired with microcomputerized
tomography scanning, we explored the covariation of vertebrae in four closely
related taxa of small‐bodied newts in the genus Lissotriton. The data were analyzed
by segmented linear regression to explore patterns of vertebral regionalization and
by a two‐block partial least squares method to test for morphological integration. All
taxa show a morphological shift posterior to the fifth trunk vertebra, which
corresponds to the two‐region concept. However, morphological integration is
found to be strongest in the mid‐trunk. Taken jointly, these results indicate a highly
integrated presacral vertebral column with a subtle two‐region differentiation. The
results are discussed in relation to specific functional requirements, developmental
and phylogenetic constraints, and specific requirements posed by a biphasic life
cycle and different locomotor modes (swimming vs. walking). Further research
should be conducted on different ontogenetic stages and closely related but
ecologically differentiated species.
PB  - John Wiley and Sons Inc
T2  - Journal of Experimental Zoology Part B - Molecular and Developmental Evolution
T1  - Regionalization and morphological integration in the vertebral column of Eurasian small‐bodied newts (Salamandridae: Lissotriton)
VL  - 2023
DO  - 10.1002/jez.b.23205
ER  - 

@article{
author = "Urošević, Aleksandar and Ajduković, Maja and Vučić, Tijana and Scholtes, Stefan and Arntzen, Jan, W and Ivanović, Ana",
year = "2023",
abstract = "Serially homologous structures may have complex patterns of regionalization and
morphological integration, influenced by developmental Hox gene expression and
functional constraints. The vertebral column, consisting of a number of repeated,
developmentally constrained, and highly integrated units—vertebrae—is such a
complex serially homologous structure. Functional diversification increases regionalization
and modularity of the vertebral column, particularly in mammals. For
salamanders, three concepts of regionalization of the vertebral column have been
proposed, recognizing one, two, or three presacral regions. Using three‐dimensional
geometric morphometrics on vertebra models acquired with microcomputerized
tomography scanning, we explored the covariation of vertebrae in four closely
related taxa of small‐bodied newts in the genus Lissotriton. The data were analyzed
by segmented linear regression to explore patterns of vertebral regionalization and
by a two‐block partial least squares method to test for morphological integration. All
taxa show a morphological shift posterior to the fifth trunk vertebra, which
corresponds to the two‐region concept. However, morphological integration is
found to be strongest in the mid‐trunk. Taken jointly, these results indicate a highly
integrated presacral vertebral column with a subtle two‐region differentiation. The
results are discussed in relation to specific functional requirements, developmental
and phylogenetic constraints, and specific requirements posed by a biphasic life
cycle and different locomotor modes (swimming vs. walking). Further research
should be conducted on different ontogenetic stages and closely related but
ecologically differentiated species.",
publisher = "John Wiley and Sons Inc",
journal = "Journal of Experimental Zoology Part B - Molecular and Developmental Evolution",
title = "Regionalization and morphological integration in the vertebral column of Eurasian small‐bodied newts (Salamandridae: Lissotriton)",
volume = "2023",
doi = "10.1002/jez.b.23205"
}

Urošević, A., Ajduković, M., Vučić, T., Scholtes, S., Arntzen, J. W.,& Ivanović, A.. (2023). Regionalization and morphological integration in the vertebral column of Eurasian small‐bodied newts (Salamandridae: Lissotriton). in Journal of Experimental Zoology Part B - Molecular and Developmental Evolution
John Wiley and Sons Inc., 2023.
https://doi.org/10.1002/jez.b.23205

Urošević A, Ajduković M, Vučić T, Scholtes S, Arntzen JW, Ivanović A. Regionalization and morphological integration in the vertebral column of Eurasian small‐bodied newts (Salamandridae: Lissotriton). in Journal of Experimental Zoology Part B - Molecular and Developmental Evolution. 2023;2023.
doi:10.1002/jez.b.23205 .

Urošević, Aleksandar, Ajduković, Maja, Vučić, Tijana, Scholtes, Stefan, Arntzen, Jan, W, Ivanović, Ana, "Regionalization and morphological integration in the vertebral column of Eurasian small‐bodied newts (Salamandridae: Lissotriton)" in Journal of Experimental Zoology Part B - Molecular and Developmental Evolution, 2023 (2023),
https://doi.org/10.1002/jez.b.23205 . .

TY  - JOUR
AU  - Anđelković, Marko
AU  - Mirč, Marko
AU  - Ajduković, Maja
AU  - Cvijanović, Milena
AU  - Vukov, Tanja
AU  - Vučić, Tijana
AU  - Kijanović, Ana
AU  - Urošević, Aleksandar
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5519
AB  - Alpine habitats are exposed to increasing anthropogenic pressure and climate change. The negative impacts can lead to chronic stress that can affect the survival and reproductive success of individuals and even lead to population extinction. In this study, we analyse different morphological and ecological traits and indices of abiotic and biotic stressors (such as head size and shape, fluctuating asymmetry, body condition index, tail autotomy, and population abundance) in alpine and subalpine populations of two lacertid species (Zootoca vivipara and Lacerta agilis) from Serbia and North Macedonia. These lizards live under different conditions: allotopy/syntopy, different anthropogenic pressure, and different levels of habitat protection. We found differences between syntopic and allotopic populations in pileus size, body condition index (in both species), pileus shape, fluctuating asymmetry (in L. agilis), and abundance (in Z. vivipara). Differences between populations under anthropogenic pressure and populations without it were observed in pileus shape, body condition index (in both species), pileus size, fluctuating asymmetry, tail autotomy and abundance (in L. agilis). On the basis of our results, it is necessary to include other stress indicators in addition to fluctuating asymmetry to quickly observe and quantify the negative effects of threat factors and apply protective measures.
PB  - Basel: MDPI
T2  - Symmetry
T1  - Is Fluctuating Asymmetry a Sufficient Indicator of Stress Level in Two Lizard Species (Zootoca vivipara and Lacerta agilis) from Alpine Habitats?
IS  - 3
VL  - 15
DO  - 10.3390/sym15030721
SP  - 721
ER  - 

@article{
author = "Anđelković, Marko and Mirč, Marko and Ajduković, Maja and Cvijanović, Milena and Vukov, Tanja and Vučić, Tijana and Kijanović, Ana and Urošević, Aleksandar",
year = "2023",
abstract = "Alpine habitats are exposed to increasing anthropogenic pressure and climate change. The negative impacts can lead to chronic stress that can affect the survival and reproductive success of individuals and even lead to population extinction. In this study, we analyse different morphological and ecological traits and indices of abiotic and biotic stressors (such as head size and shape, fluctuating asymmetry, body condition index, tail autotomy, and population abundance) in alpine and subalpine populations of two lacertid species (Zootoca vivipara and Lacerta agilis) from Serbia and North Macedonia. These lizards live under different conditions: allotopy/syntopy, different anthropogenic pressure, and different levels of habitat protection. We found differences between syntopic and allotopic populations in pileus size, body condition index (in both species), pileus shape, fluctuating asymmetry (in L. agilis), and abundance (in Z. vivipara). Differences between populations under anthropogenic pressure and populations without it were observed in pileus shape, body condition index (in both species), pileus size, fluctuating asymmetry, tail autotomy and abundance (in L. agilis). On the basis of our results, it is necessary to include other stress indicators in addition to fluctuating asymmetry to quickly observe and quantify the negative effects of threat factors and apply protective measures.",
publisher = "Basel: MDPI",
journal = "Symmetry",
title = "Is Fluctuating Asymmetry a Sufficient Indicator of Stress Level in Two Lizard Species (Zootoca vivipara and Lacerta agilis) from Alpine Habitats?",
number = "3",
volume = "15",
doi = "10.3390/sym15030721",
pages = "721"
}

Anđelković, M., Mirč, M., Ajduković, M., Cvijanović, M., Vukov, T., Vučić, T., Kijanović, A.,& Urošević, A.. (2023). Is Fluctuating Asymmetry a Sufficient Indicator of Stress Level in Two Lizard Species (Zootoca vivipara and Lacerta agilis) from Alpine Habitats?. in Symmetry
Basel: MDPI., 15(3), 721.
https://doi.org/10.3390/sym15030721

Anđelković M, Mirč M, Ajduković M, Cvijanović M, Vukov T, Vučić T, Kijanović A, Urošević A. Is Fluctuating Asymmetry a Sufficient Indicator of Stress Level in Two Lizard Species (Zootoca vivipara and Lacerta agilis) from Alpine Habitats?. in Symmetry. 2023;15(3):721.
doi:10.3390/sym15030721 .

Anđelković, Marko, Mirč, Marko, Ajduković, Maja, Cvijanović, Milena, Vukov, Tanja, Vučić, Tijana, Kijanović, Ana, Urošević, Aleksandar, "Is Fluctuating Asymmetry a Sufficient Indicator of Stress Level in Two Lizard Species (Zootoca vivipara and Lacerta agilis) from Alpine Habitats?" in Symmetry, 15, no. 3 (2023):721,
https://doi.org/10.3390/sym15030721 . .

TY  - JOUR
AU  - Ivanović, Ana
AU  - Cvijanović, Milena
AU  - Vučić, Tijana
AU  - Arntzen, Jan W.
PY  - 2023
UR  - https://link.springer.com/10.1007/s13127-022-00585-5
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5167
AB  - We examined the cranial morphology and cranial kinesis of the common toads Bufo bufo and B. spinosus with micro-computed tomography and geometric morphometrics and compared the results with published data for related species in a phylogenetic context. The species significantly diverge in skull shape. The skull of B. spinosus is shorter and higher, with a ventral arm of the squamosal bone and the jaw articulation point positioned perpendicular to the braincase, in comparison with a more lateral position in B. bufo . In either species, females have a shorter snout and a higher and wider skull at the jaw articulation point that is positioned more posteriorly, in comparison with conspecific males. High variation in the amount of bone ossification was recorded in both species, ranging from scarcely ossified and loosely connected bones to highly ossified and firmly connected bones. We also found that skull shape and inferred kinetic properties of the skull are highly variable across the Bufonini tribe. However, sample sizes are mostly small and intraspecific variation is high, which might compromise the analyses. Overall, the results suggest that developmental plasticity produces high variation in ossification and cranial kinesis, affecting individuals’ feeding performances. At the population level, this variation supports an efficient exploitation of the habitat and may promote morphological adaptation in a changing environment.
PB  - Heidelberg: Springer Heidelberg
T2  - Organisms Diversity & Evolution
T1  - Differentiation of skull morphology and cranial kinesis in common toads
VL  - 23
DO  - 10.1007/s13127-022-00585-5
SP  - 209
EP  - 219
ER  - 

@article{
author = "Ivanović, Ana and Cvijanović, Milena and Vučić, Tijana and Arntzen, Jan W.",
year = "2023",
abstract = "We examined the cranial morphology and cranial kinesis of the common toads Bufo bufo and B. spinosus with micro-computed tomography and geometric morphometrics and compared the results with published data for related species in a phylogenetic context. The species significantly diverge in skull shape. The skull of B. spinosus is shorter and higher, with a ventral arm of the squamosal bone and the jaw articulation point positioned perpendicular to the braincase, in comparison with a more lateral position in B. bufo . In either species, females have a shorter snout and a higher and wider skull at the jaw articulation point that is positioned more posteriorly, in comparison with conspecific males. High variation in the amount of bone ossification was recorded in both species, ranging from scarcely ossified and loosely connected bones to highly ossified and firmly connected bones. We also found that skull shape and inferred kinetic properties of the skull are highly variable across the Bufonini tribe. However, sample sizes are mostly small and intraspecific variation is high, which might compromise the analyses. Overall, the results suggest that developmental plasticity produces high variation in ossification and cranial kinesis, affecting individuals’ feeding performances. At the population level, this variation supports an efficient exploitation of the habitat and may promote morphological adaptation in a changing environment.",
publisher = "Heidelberg: Springer Heidelberg",
journal = "Organisms Diversity & Evolution",
title = "Differentiation of skull morphology and cranial kinesis in common toads",
volume = "23",
doi = "10.1007/s13127-022-00585-5",
pages = "209-219"
}

Ivanović, A., Cvijanović, M., Vučić, T.,& Arntzen, J. W.. (2023). Differentiation of skull morphology and cranial kinesis in common toads. in Organisms Diversity & Evolution
Heidelberg: Springer Heidelberg., 23, 209-219.
https://doi.org/10.1007/s13127-022-00585-5

Ivanović A, Cvijanović M, Vučić T, Arntzen JW. Differentiation of skull morphology and cranial kinesis in common toads. in Organisms Diversity & Evolution. 2023;23:209-219.
doi:10.1007/s13127-022-00585-5 .

Ivanović, Ana, Cvijanović, Milena, Vučić, Tijana, Arntzen, Jan W., "Differentiation of skull morphology and cranial kinesis in common toads" in Organisms Diversity & Evolution, 23 (2023):209-219,
https://doi.org/10.1007/s13127-022-00585-5 . .

TY  - GEN
AU  - Urošević, Aleksandar
AU  - Anđelković, Marko
AU  - Maričić, Marko
AU  - Vučić, Tijana
AU  - Šević, Mirko
AU  - Tokić, Goran
AU  - Tomović, Ljiljana
PY  - 2023
UR  - https://www.biotaxa.org/hn/article/view/77476
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5397
PB  - Bonn: Societas Europaea Herpetologica
T2  - Herpetology Notes
T1  - Further introductions and population establishment of Hemidactylus turcicus (Linnaeus, 1758) in Serbia (Squamata: Gekkonidae)
VL  - 16
SP  - 21
EP  - 24
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5397
ER  - 

@misc{
author = "Urošević, Aleksandar and Anđelković, Marko and Maričić, Marko and Vučić, Tijana and Šević, Mirko and Tokić, Goran and Tomović, Ljiljana",
year = "2023",
publisher = "Bonn: Societas Europaea Herpetologica",
journal = "Herpetology Notes",
title = "Further introductions and population establishment of Hemidactylus turcicus (Linnaeus, 1758) in Serbia (Squamata: Gekkonidae)",
volume = "16",
pages = "21-24",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5397"
}

Urošević, A., Anđelković, M., Maričić, M., Vučić, T., Šević, M., Tokić, G.,& Tomović, L.. (2023). Further introductions and population establishment of Hemidactylus turcicus (Linnaeus, 1758) in Serbia (Squamata: Gekkonidae). in Herpetology Notes
Bonn: Societas Europaea Herpetologica., 16, 21-24.
https://hdl.handle.net/21.15107/rcub_ibiss_5397

Urošević A, Anđelković M, Maričić M, Vučić T, Šević M, Tokić G, Tomović L. Further introductions and population establishment of Hemidactylus turcicus (Linnaeus, 1758) in Serbia (Squamata: Gekkonidae). in Herpetology Notes. 2023;16:21-24.
https://hdl.handle.net/21.15107/rcub_ibiss_5397 .

Urošević, Aleksandar, Anđelković, Marko, Maričić, Marko, Vučić, Tijana, Šević, Mirko, Tokić, Goran, Tomović, Ljiljana, "Further introductions and population establishment of Hemidactylus turcicus (Linnaeus, 1758) in Serbia (Squamata: Gekkonidae)" in Herpetology Notes, 16 (2023):21-24,
https://hdl.handle.net/21.15107/rcub_ibiss_5397 .

TY  - JOUR
AU  - Cvijanović, Milena
AU  - Ajduković, Maja
AU  - Arntzen, Jan Willem
AU  - Ivanović, Ana
AU  - Vučić, Tijana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5474
AB  - Micro-computed tomography is a powerful tool toward the detailed reconstruction of internal and external morphology, in particular for ossified and other dense tissues. Here, we document and compare the level of calcification in the skin of the head and the parotoids (the external skin glands) in males and females of common and spined toads, Bufo bufo and B. spinosus. In some anurans, including Bufo species, a specific acellular calcified tissue layer within the dermis has been documented (the Eberth-Katschenko, or EK-layer). By a combination of micro-computed tomography and classical histology, we detected additional calcium deposits located in the dermal layer stratum spongiosum, positioned above the EK-layer. We showed that the level of calcification and the presence of additional calcium deposits are size and sex related, increasing in the order B. bufo males, B. spinosus males, B. bufo females to B. spinosus females. The last of these groups is the least variable. Bufo spinosus females have dense calcium deposits in the parotoids and the dorsal and ventral skin. Three-dimensional volume renderings and cross-sectional slices obtained by micro-CT scanning indicate that this approach is a promising technique for further studies on bufonid skin anatomy and geographic variation in skin calcification.
PB  - John Wiley and Sons Inc
T2  - The Anatomical Record
T1  - Ladies in armor: A micro-computed tomographic study of skin calcification in European toads (genus Bufo)
DO  - 10.1002/ar.25170
ER  - 

@article{
author = "Cvijanović, Milena and Ajduković, Maja and Arntzen, Jan Willem and Ivanović, Ana and Vučić, Tijana",
year = "2023",
abstract = "Micro-computed tomography is a powerful tool toward the detailed reconstruction of internal and external morphology, in particular for ossified and other dense tissues. Here, we document and compare the level of calcification in the skin of the head and the parotoids (the external skin glands) in males and females of common and spined toads, Bufo bufo and B. spinosus. In some anurans, including Bufo species, a specific acellular calcified tissue layer within the dermis has been documented (the Eberth-Katschenko, or EK-layer). By a combination of micro-computed tomography and classical histology, we detected additional calcium deposits located in the dermal layer stratum spongiosum, positioned above the EK-layer. We showed that the level of calcification and the presence of additional calcium deposits are size and sex related, increasing in the order B. bufo males, B. spinosus males, B. bufo females to B. spinosus females. The last of these groups is the least variable. Bufo spinosus females have dense calcium deposits in the parotoids and the dorsal and ventral skin. Three-dimensional volume renderings and cross-sectional slices obtained by micro-CT scanning indicate that this approach is a promising technique for further studies on bufonid skin anatomy and geographic variation in skin calcification.",
publisher = "John Wiley and Sons Inc",
journal = "The Anatomical Record",
title = "Ladies in armor: A micro-computed tomographic study of skin calcification in European toads (genus Bufo)",
doi = "10.1002/ar.25170"
}

Cvijanović, M., Ajduković, M., Arntzen, J. W., Ivanović, A.,& Vučić, T.. (2023). Ladies in armor: A micro-computed tomographic study of skin calcification in European toads (genus Bufo). in The Anatomical Record
John Wiley and Sons Inc..
https://doi.org/10.1002/ar.25170

Cvijanović M, Ajduković M, Arntzen JW, Ivanović A, Vučić T. Ladies in armor: A micro-computed tomographic study of skin calcification in European toads (genus Bufo). in The Anatomical Record. 2023;.
doi:10.1002/ar.25170 .

Cvijanović, Milena, Ajduković, Maja, Arntzen, Jan Willem, Ivanović, Ana, Vučić, Tijana, "Ladies in armor: A micro-computed tomographic study of skin calcification in European toads (genus Bufo)" in The Anatomical Record (2023),
https://doi.org/10.1002/ar.25170 . .

TY  - JOUR
AU  - Petrović, Tamara
AU  - Vučić, Tijana
AU  - Burraco, Pablo
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Radovanović, Tijana
AU  - Šajkunić, Sanja
AU  - Ivanović, Ana
AU  - Prokić, Marko
PY  - 2023
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0306456523000153
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5463
AB  - Ectotherms are particularly sensitive to global warming due to their limited capacity to thermoregulate, which can impact their performance and fitness. From a physiological standpoint, higher temperatures often enhance biological processes that can induce the production of reactive oxygen species and result in a state of cellular oxidative stress. Temperature alters interspecific interactions, including species hybridization. Hybridization under different thermal conditions could amplify parental (genetic) incompatibilities, thus affecting a hybrid's development and distribution. Understanding the impact of global warming on the physiology of hybrids and particularly their oxidative status could help in predicting future scenarios in ecosystems and in hybrids. In the present study, we investigated the effect of water temperature on the development, growth and oxidative stress of two crested newt species and their reciprocal hybrids. Larvae of Triturus macedonicus and T. ivanbureschi, and their T. macedonicus-mothered and T. ivanbureschi-mothered hybrids were exposed for 30 days to temperatures of 19°C and 24°C. Under the higher temperature, the hybrids experienced increases in both growth and developmental rates, while parental species exhibited accelerated growth (T. macedonicus) or development (T. ivanbureschi). Warm conditions also had different effects on the oxidative status of hybrid and parental species. Parental species had enhanced antioxidant responses (catalase, glutathione peroxidase, glutathione S-transferase and SH groups), which allowed them to alleviate temperature-induced stress (revealed by the absence of oxidative damage). However, warming induced an antioxidant response in the hybrids, including oxidative damage in the form of lipid peroxidation. These findings point to a greater disruption of redox regulation and metabolic machinery in hybrid newts, which can be interpreted as the cost of hybridization that is likely linked to parental incompatibilities expressed under a higher temperature. Our study aims to improve mechanistic understanding of the resilience and distribution of hybrid species that cope with climate-driven changes.
PB  - Elsevier Ltd
T2  - Journal of Thermal Biology
T1  - Higher temperature induces oxidative stress in hybrids but not in parental species: A case study of crested newts
VL  - 112
DO  - 10.1016/j.jtherbio.2023.103474
SP  - 103474
ER  - 

@article{
author = "Petrović, Tamara and Vučić, Tijana and Burraco, Pablo and Gavrilović, Branka and Despotović, Svetlana and Gavrić, Jelena and Radovanović, Tijana and Šajkunić, Sanja and Ivanović, Ana and Prokić, Marko",
year = "2023",
abstract = "Ectotherms are particularly sensitive to global warming due to their limited capacity to thermoregulate, which can impact their performance and fitness. From a physiological standpoint, higher temperatures often enhance biological processes that can induce the production of reactive oxygen species and result in a state of cellular oxidative stress. Temperature alters interspecific interactions, including species hybridization. Hybridization under different thermal conditions could amplify parental (genetic) incompatibilities, thus affecting a hybrid's development and distribution. Understanding the impact of global warming on the physiology of hybrids and particularly their oxidative status could help in predicting future scenarios in ecosystems and in hybrids. In the present study, we investigated the effect of water temperature on the development, growth and oxidative stress of two crested newt species and their reciprocal hybrids. Larvae of Triturus macedonicus and T. ivanbureschi, and their T. macedonicus-mothered and T. ivanbureschi-mothered hybrids were exposed for 30 days to temperatures of 19°C and 24°C. Under the higher temperature, the hybrids experienced increases in both growth and developmental rates, while parental species exhibited accelerated growth (T. macedonicus) or development (T. ivanbureschi). Warm conditions also had different effects on the oxidative status of hybrid and parental species. Parental species had enhanced antioxidant responses (catalase, glutathione peroxidase, glutathione S-transferase and SH groups), which allowed them to alleviate temperature-induced stress (revealed by the absence of oxidative damage). However, warming induced an antioxidant response in the hybrids, including oxidative damage in the form of lipid peroxidation. These findings point to a greater disruption of redox regulation and metabolic machinery in hybrid newts, which can be interpreted as the cost of hybridization that is likely linked to parental incompatibilities expressed under a higher temperature. Our study aims to improve mechanistic understanding of the resilience and distribution of hybrid species that cope with climate-driven changes.",
publisher = "Elsevier Ltd",
journal = "Journal of Thermal Biology",
title = "Higher temperature induces oxidative stress in hybrids but not in parental species: A case study of crested newts",
volume = "112",
doi = "10.1016/j.jtherbio.2023.103474",
pages = "103474"
}

Petrović, T., Vučić, T., Burraco, P., Gavrilović, B., Despotović, S., Gavrić, J., Radovanović, T., Šajkunić, S., Ivanović, A.,& Prokić, M.. (2023). Higher temperature induces oxidative stress in hybrids but not in parental species: A case study of crested newts. in Journal of Thermal Biology
Elsevier Ltd., 112, 103474.
https://doi.org/10.1016/j.jtherbio.2023.103474

Petrović T, Vučić T, Burraco P, Gavrilović B, Despotović S, Gavrić J, Radovanović T, Šajkunić S, Ivanović A, Prokić M. Higher temperature induces oxidative stress in hybrids but not in parental species: A case study of crested newts. in Journal of Thermal Biology. 2023;112:103474.
doi:10.1016/j.jtherbio.2023.103474 .

Petrović, Tamara, Vučić, Tijana, Burraco, Pablo, Gavrilović, Branka, Despotović, Svetlana, Gavrić, Jelena, Radovanović, Tijana, Šajkunić, Sanja, Ivanović, Ana, Prokić, Marko, "Higher temperature induces oxidative stress in hybrids but not in parental species: A case study of crested newts" in Journal of Thermal Biology, 112 (2023):103474,
https://doi.org/10.1016/j.jtherbio.2023.103474 . .

TY  - JOUR
AU  - Ajduković, Maja
AU  - Vučić, Tijana
AU  - Cvijanović, Milena
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5032
AB  - Amphibians are useful bioindicators for monitoring aquatic health and the influence of xenobiotics such as endocrine disrupting chemicals. Because aquatic ecosystems experience the majority of global pollution, aquatic organisms are most exposed and vulnerable to endocrine disruptors. Furthermore, penetration of endocrine disruptors into aquatic organisms especially in amphibians is even easier because of more permeable skin, resulting in high bioavailability and bioaccumulation of chemicals. One of the most potent endocrine disruptors is thiourea, which chemically blocks the synthesis of thyroid hormones and prevents metamorphosis in amphibians. We investigated the influence of thiourea on histomorphology of the thyroid gland in Triturus newts at the metamorphic stage, when thyroid hormone concentrations should reach their maximum level. Chronic exposure to thiourea induced hypertrophy and hyperplasia of follicular cells as well as a significant reduction of interstitial tissue. The intensity of the thyroglobulin immunostaining signal significantly decreases upon chronic exposure to thiourea. Successful cross-reactivity of human primary antibody in immunochemical detection of thyroglobulin in Urodela confirms potential homology in thyroglobulin structure throughout the vertebrates.
PB  - Munich: Elsevier GmbH
T2  - Zoology
T1  - Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts
VL  - 155
DO  - 10.1016/j.zool.2022.126050
SP  - 126050
ER  - 

@article{
author = "Ajduković, Maja and Vučić, Tijana and Cvijanović, Milena and Filipović, Branko and Šošić-Jurjević, Branka ",
year = "2022",
abstract = "Amphibians are useful bioindicators for monitoring aquatic health and the influence of xenobiotics such as endocrine disrupting chemicals. Because aquatic ecosystems experience the majority of global pollution, aquatic organisms are most exposed and vulnerable to endocrine disruptors. Furthermore, penetration of endocrine disruptors into aquatic organisms especially in amphibians is even easier because of more permeable skin, resulting in high bioavailability and bioaccumulation of chemicals. One of the most potent endocrine disruptors is thiourea, which chemically blocks the synthesis of thyroid hormones and prevents metamorphosis in amphibians. We investigated the influence of thiourea on histomorphology of the thyroid gland in Triturus newts at the metamorphic stage, when thyroid hormone concentrations should reach their maximum level. Chronic exposure to thiourea induced hypertrophy and hyperplasia of follicular cells as well as a significant reduction of interstitial tissue. The intensity of the thyroglobulin immunostaining signal significantly decreases upon chronic exposure to thiourea. Successful cross-reactivity of human primary antibody in immunochemical detection of thyroglobulin in Urodela confirms potential homology in thyroglobulin structure throughout the vertebrates.",
publisher = "Munich: Elsevier GmbH",
journal = "Zoology",
title = "Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts",
volume = "155",
doi = "10.1016/j.zool.2022.126050",
pages = "126050"
}

Ajduković, M., Vučić, T., Cvijanović, M., Filipović, B.,& Šošić-Jurjević, B.. (2022). Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts. in Zoology
Munich: Elsevier GmbH., 155, 126050.
https://doi.org/10.1016/j.zool.2022.126050

Ajduković M, Vučić T, Cvijanović M, Filipović B, Šošić-Jurjević B. Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts. in Zoology. 2022;155:126050.
doi:10.1016/j.zool.2022.126050 .

Ajduković, Maja, Vučić, Tijana, Cvijanović, Milena, Filipović, Branko, Šošić-Jurjević, Branka , "Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts" in Zoology, 155 (2022):126050,
https://doi.org/10.1016/j.zool.2022.126050 . .

TY  - JOUR
AU  - Bugarčić, Marko
AU  - Ivanović, Ana
AU  - Cvijanović, Milena
AU  - Vučić, Tijana
PY  - 2022
UR  - https://brill.com/view/journals/amre/aop/article-10.1163-15685381-bja10082/article-10.1163-15685381-bja10082.xml
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4873
AB  - The Macedonian crested newt T. macedonicus forms a complex hybrid zone with the Balkan crested newt T. ivanbureschi in the central part of the Balkan Peninsula. In this study, we compared life history parameters (sex ratio, survival rate and age of sexual maturation) and growth parameters (body length and mass) of T. macedonicus and T. macedonicus × T. ivanbureschi hybrids over the first three postmetamorphic years. Survival rates were high and similar for both genotypes. Sexual dimorphism in body size (length and mass) was evident at the beginning of the third postmetamorphic year, after the first breeding year, for both T. macedonicus and hybrids. We did not find clear and consistent difference in any of the analysed traits suggesting that hybridization with T. ivabureschi does not affect postmetamorphic growth and survival of T. macedonicus .
PB  - Brill Academic Publishers
T2  - Amphibia-Reptilia
T1  - Consequences of hybridization on life history and growth in postmetamorphic Triturus macedonicus
IS  - 5
VL  - 10
DO  - 10.1163/15685381-bja10082
SP  - 1
EP  - 7
ER  - 

@article{
author = "Bugarčić, Marko and Ivanović, Ana and Cvijanović, Milena and Vučić, Tijana",
year = "2022",
abstract = "The Macedonian crested newt T. macedonicus forms a complex hybrid zone with the Balkan crested newt T. ivanbureschi in the central part of the Balkan Peninsula. In this study, we compared life history parameters (sex ratio, survival rate and age of sexual maturation) and growth parameters (body length and mass) of T. macedonicus and T. macedonicus × T. ivanbureschi hybrids over the first three postmetamorphic years. Survival rates were high and similar for both genotypes. Sexual dimorphism in body size (length and mass) was evident at the beginning of the third postmetamorphic year, after the first breeding year, for both T. macedonicus and hybrids. We did not find clear and consistent difference in any of the analysed traits suggesting that hybridization with T. ivabureschi does not affect postmetamorphic growth and survival of T. macedonicus .",
publisher = "Brill Academic Publishers",
journal = "Amphibia-Reptilia",
title = "Consequences of hybridization on life history and growth in postmetamorphic Triturus macedonicus",
number = "5",
volume = "10",
doi = "10.1163/15685381-bja10082",
pages = "1-7"
}

Bugarčić, M., Ivanović, A., Cvijanović, M.,& Vučić, T.. (2022). Consequences of hybridization on life history and growth in postmetamorphic Triturus macedonicus. in Amphibia-Reptilia
Brill Academic Publishers., 10(5), 1-7.
https://doi.org/10.1163/15685381-bja10082

Bugarčić M, Ivanović A, Cvijanović M, Vučić T. Consequences of hybridization on life history and growth in postmetamorphic Triturus macedonicus. in Amphibia-Reptilia. 2022;10(5):1-7.
doi:10.1163/15685381-bja10082 .

Bugarčić, Marko, Ivanović, Ana, Cvijanović, Milena, Vučić, Tijana, "Consequences of hybridization on life history and growth in postmetamorphic Triturus macedonicus" in Amphibia-Reptilia, 10, no. 5 (2022):1-7,
https://doi.org/10.1163/15685381-bja10082 . .

TY  - CONF
AU  - Ajduković, Maja
AU  - Vučić, Tijana
AU  - Cvijanović, Milena
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5227
AB  - Aquatic ecosystems receive the majority of global pollution. Therefore, aquatic
organisms are the most exposed and the most vulnerable as penetration of various
endocrine disruptors is easier, resulting in high bioavailability and bioaccumulation of
chemicals. One of the potent endocrine disruptors (anti-thyroid agents) is thiourea,
which chemically blocks the synthesis of thyroid hormones and prevents
metamorphosis in amphibians. The mechanism of action is very similar for all antithyroid agents via inhibition of thyroid peroxidase enzyme (TPO), thereby inhibiting
iodination of tyrosine residues in thyroglobulin, the oxidative coupling of iodinated
tyrosine and therefore the biosynthesis of the thyroid hormones. We investigated the
influence of two non-lethal concentrations of thiourea (0.05% and 0.1%) on
histomorphology of the thyroid gland in Triturus newts at the metamorphic stage,
when TH concentrations should reach maximum level. Exposure to thiourea induced
hypertrophy and hyperplasia of follicular cells as well as a significant reduction
(p<0.05) of interstitial tissue. Intensity of thyroglobulin immunostaining significantly
decreased (p<0.05) upon both thiourea treatments. Additionally, we tested
mammalian thyroglobulin antibodies for immunohistochemical assessment of the
thyroid follicular tissue in newts. Successful cross-reactivity of human primary
antibody in immunochemical detection of thyroglobulin in Triturus newts in this study
confirms potential homology in protein structure throughout the vertebrates.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
T1  - Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts
SP  - 41
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5227
ER  - 

@conference{
author = "Ajduković, Maja and Vučić, Tijana and Cvijanović, Milena and Filipović, Branko and Šošić-Jurjević, Branka ",
year = "2022",
abstract = "Aquatic ecosystems receive the majority of global pollution. Therefore, aquatic
organisms are the most exposed and the most vulnerable as penetration of various
endocrine disruptors is easier, resulting in high bioavailability and bioaccumulation of
chemicals. One of the potent endocrine disruptors (anti-thyroid agents) is thiourea,
which chemically blocks the synthesis of thyroid hormones and prevents
metamorphosis in amphibians. The mechanism of action is very similar for all antithyroid agents via inhibition of thyroid peroxidase enzyme (TPO), thereby inhibiting
iodination of tyrosine residues in thyroglobulin, the oxidative coupling of iodinated
tyrosine and therefore the biosynthesis of the thyroid hormones. We investigated the
influence of two non-lethal concentrations of thiourea (0.05% and 0.1%) on
histomorphology of the thyroid gland in Triturus newts at the metamorphic stage,
when TH concentrations should reach maximum level. Exposure to thiourea induced
hypertrophy and hyperplasia of follicular cells as well as a significant reduction
(p<0.05) of interstitial tissue. Intensity of thyroglobulin immunostaining significantly
decreased (p<0.05) upon both thiourea treatments. Additionally, we tested
mammalian thyroglobulin antibodies for immunohistochemical assessment of the
thyroid follicular tissue in newts. Successful cross-reactivity of human primary
antibody in immunochemical detection of thyroglobulin in Triturus newts in this study
confirms potential homology in protein structure throughout the vertebrates.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia",
title = "Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts",
pages = "41",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5227"
}

Ajduković, M., Vučić, T., Cvijanović, M., Filipović, B.,& Šošić-Jurjević, B.. (2022). Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 41.
https://hdl.handle.net/21.15107/rcub_ibiss_5227

Ajduković M, Vučić T, Cvijanović M, Filipović B, Šošić-Jurjević B. Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. 2022;:41.
https://hdl.handle.net/21.15107/rcub_ibiss_5227 .

Ajduković, Maja, Vučić, Tijana, Cvijanović, Milena, Filipović, Branko, Šošić-Jurjević, Branka , "Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts" in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia (2022):41,
https://hdl.handle.net/21.15107/rcub_ibiss_5227 .

TY  - JOUR
AU  - Vučić, Tijana
AU  - Ivanović, Ana
AU  - Ajduković, Maja
AU  - Bajler, Nikola
AU  - Cvijanović, Milena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4866
AB  - Two large-bodied newt species, Triturus ivanbureschi and T. macedonicus, hybridize in nature across the Balkan Peninsula. Consequences of hybridization upon secondary contact of two species include species displacement and asymmetrical introgression of T. ivanbureschi mtDNA. We set an experimental reciprocal cross of parental species and obtained two genotypes of F1 hybrids (with T. ivanbureschi or T. macedonicus mtDNA). When hybrids attained sexual maturity, they were engaged in mutual crossings and backcrossing with parental species. We followed reproductive traits over two successive years. Our main aim was to explore the reproductive success of F1 females carrying different parental mtDNA. Additionally, we tested for differences in reproductive success within female genotypes depending on the crossing with various male genotypes (hybrids or parental species). Both female genotypes had similar oviposition periods, number of laid eggs and hatched larvae but different body and egg sizes. Overall reproductive success (percentage of egg-laying females and viability of embryos) was similar for both genotypes. The type of crossing led to some differences in reproductive success within female genotypes. The obtained results suggest that processes that led to exclusion of T. macedonicus mtDNA in natural populations may be related to the survival at postembryonic stages of F2 generation or reproductive barriers that emerged in subsequent hybrid generations.
PB  - Basel: MDPI
T2  - Animals
T1  - The reproductive success of Triturus ivanbureschi x T. macedonicus F1 hzbrid females (Amphibia:Salamandridae)
IS  - 4
VL  - 12
DO  - 10.3390/ani12040443
SP  - 443
ER  - 

@article{
author = "Vučić, Tijana and Ivanović, Ana and Ajduković, Maja and Bajler, Nikola and Cvijanović, Milena",
year = "2022",
abstract = "Two large-bodied newt species, Triturus ivanbureschi and T. macedonicus, hybridize in nature across the Balkan Peninsula. Consequences of hybridization upon secondary contact of two species include species displacement and asymmetrical introgression of T. ivanbureschi mtDNA. We set an experimental reciprocal cross of parental species and obtained two genotypes of F1 hybrids (with T. ivanbureschi or T. macedonicus mtDNA). When hybrids attained sexual maturity, they were engaged in mutual crossings and backcrossing with parental species. We followed reproductive traits over two successive years. Our main aim was to explore the reproductive success of F1 females carrying different parental mtDNA. Additionally, we tested for differences in reproductive success within female genotypes depending on the crossing with various male genotypes (hybrids or parental species). Both female genotypes had similar oviposition periods, number of laid eggs and hatched larvae but different body and egg sizes. Overall reproductive success (percentage of egg-laying females and viability of embryos) was similar for both genotypes. The type of crossing led to some differences in reproductive success within female genotypes. The obtained results suggest that processes that led to exclusion of T. macedonicus mtDNA in natural populations may be related to the survival at postembryonic stages of F2 generation or reproductive barriers that emerged in subsequent hybrid generations.",
publisher = "Basel: MDPI",
journal = "Animals",
title = "The reproductive success of Triturus ivanbureschi x T. macedonicus F1 hzbrid females (Amphibia:Salamandridae)",
number = "4",
volume = "12",
doi = "10.3390/ani12040443",
pages = "443"
}

Vučić, T., Ivanović, A., Ajduković, M., Bajler, N.,& Cvijanović, M.. (2022). The reproductive success of Triturus ivanbureschi x T. macedonicus F1 hzbrid females (Amphibia:Salamandridae). in Animals
Basel: MDPI., 12(4), 443.
https://doi.org/10.3390/ani12040443

Vučić T, Ivanović A, Ajduković M, Bajler N, Cvijanović M. The reproductive success of Triturus ivanbureschi x T. macedonicus F1 hzbrid females (Amphibia:Salamandridae). in Animals. 2022;12(4):443.
doi:10.3390/ani12040443 .

Vučić, Tijana, Ivanović, Ana, Ajduković, Maja, Bajler, Nikola, Cvijanović, Milena, "The reproductive success of Triturus ivanbureschi x T. macedonicus F1 hzbrid females (Amphibia:Salamandridae)" in Animals, 12, no. 4 (2022):443,
https://doi.org/10.3390/ani12040443 . .

TY  - JOUR
AU  - Tomović, Ljiljana
AU  - Vučić, Tijana
AU  - Anđelković, Marko
AU  - Urošević, Aleksandar
AU  - Bjelica, Vukašin
AU  - Maričić, Marko
AU  - Lakušić, Margareta
AU  - Danon, Gorana
AU  - Ivanović, Ana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5548
AB  - We present the updated distribution of batracho- and herpetofauna in southern
and south-eastern Serbia, based on literature and new field records for 16
amphibian and 22 reptile species. In these two biogeographic regions, already
recognized as biodiversity hotspots, we discovered two new amphibian species
with limited distribution: Hyla orientalis and Pelobates balcanicus. Also, one
species (Triturus ivanbureshi) has restricted distribution, while one (Bombina
bombina) is rare. Four reptile species have limited distributions and six are rare in
these regions. The presence of hybrid zones and the influence of the Mediterranean
climate make southern and south-eastern Serbia areas of great importance and the
target for future ecological and conservation studies in Serbia.
AB  - У овом раду дато је ажурирано и систематизовано распрострањење
батрахо и херепетофауне јужне и југоисточне Србије, на основу
публикованих литературних, као и нових теренских података за 16
врста водоземаца и 22 врсте гмизаваца. У ова два биогеографска
региона, претходно препознатих као подручја високог специјског
диверзитета, забележене су две нове врсте водоземаца са ограниченим
распрострањењем: Hyla orientalis и Pelobates balcanicus. Такође, једна
врста репатих водоземца (Triturus ivanbureshi) има ограничено распрострањење, док је Bombina bombina веома ретка. Четири врсте
гмизаваца су са ограниченом дистрибуцијом, а још шест су веома
ретке у ова два биогеографска региона.
Од укупно 38 врста водоземаца и гмизаваца потврђених у регионима јужне и југоисточне Србије, шест је рањиво/готово угрожено према
критеријумима IUCN-а, осам су на анексу II Директиве о стаништима,
а две су на апендиксу II CITES-а. На националном нивоу, 10 врста се
сматра критично угроженим, угроженим или рањивим по критеријумима IUCN-а, три врсте су заштићене, а 29 строго заштићене законом.
Као резултат ових истраживања, две локације у јужној Србији:
Левосоје (Бујановац) и Славујевац (Прешево), предложене су за
заштиту у окивиру успостављања националних еколошких мрежа у
Србији.
Присуство хибридних зона и утицаја Медитеранске климе чине
регионе јужне и југоисточне Србије подручјима од великог значаја за
диверзитет наше земље, и фокусом за будуће еколошке и конзервационе студије.
PB  - Belgrade: Natural History Museum
T2  - Bulletin of the Natural History Museum
T1  - Contribution to knowledge of batracho- and herpetofauna of southern and south-eastern Serbia
T1  - Прилог познавању батрахо- и херпетофауне југоисточне и јужне Србије
VL  - 15
DO  - 10.5937/bnhmb2215171T
SP  - 171
EP  - 189
ER  - 

@article{
author = "Tomović, Ljiljana and Vučić, Tijana and Anđelković, Marko and Urošević, Aleksandar and Bjelica, Vukašin and Maričić, Marko and Lakušić, Margareta and Danon, Gorana and Ivanović, Ana",
year = "2022",
abstract = "We present the updated distribution of batracho- and herpetofauna in southern
and south-eastern Serbia, based on literature and new field records for 16
amphibian and 22 reptile species. In these two biogeographic regions, already
recognized as biodiversity hotspots, we discovered two new amphibian species
with limited distribution: Hyla orientalis and Pelobates balcanicus. Also, one
species (Triturus ivanbureshi) has restricted distribution, while one (Bombina
bombina) is rare. Four reptile species have limited distributions and six are rare in
these regions. The presence of hybrid zones and the influence of the Mediterranean
climate make southern and south-eastern Serbia areas of great importance and the
target for future ecological and conservation studies in Serbia., У овом раду дато је ажурирано и систематизовано распрострањење
батрахо и херепетофауне јужне и југоисточне Србије, на основу
публикованих литературних, као и нових теренских података за 16
врста водоземаца и 22 врсте гмизаваца. У ова два биогеографска
региона, претходно препознатих као подручја високог специјског
диверзитета, забележене су две нове врсте водоземаца са ограниченим
распрострањењем: Hyla orientalis и Pelobates balcanicus. Такође, једна
врста репатих водоземца (Triturus ivanbureshi) има ограничено распрострањење, док је Bombina bombina веома ретка. Четири врсте
гмизаваца су са ограниченом дистрибуцијом, а још шест су веома
ретке у ова два биогеографска региона.
Од укупно 38 врста водоземаца и гмизаваца потврђених у регионима јужне и југоисточне Србије, шест је рањиво/готово угрожено према
критеријумима IUCN-а, осам су на анексу II Директиве о стаништима,
а две су на апендиксу II CITES-а. На националном нивоу, 10 врста се
сматра критично угроженим, угроженим или рањивим по критеријумима IUCN-а, три врсте су заштићене, а 29 строго заштићене законом.
Као резултат ових истраживања, две локације у јужној Србији:
Левосоје (Бујановац) и Славујевац (Прешево), предложене су за
заштиту у окивиру успостављања националних еколошких мрежа у
Србији.
Присуство хибридних зона и утицаја Медитеранске климе чине
регионе јужне и југоисточне Србије подручјима од великог значаја за
диверзитет наше земље, и фокусом за будуће еколошке и конзервационе студије.",
publisher = "Belgrade: Natural History Museum",
journal = "Bulletin of the Natural History Museum",
title = "Contribution to knowledge of batracho- and herpetofauna of southern and south-eastern Serbia, Прилог познавању батрахо- и херпетофауне југоисточне и јужне Србије",
volume = "15",
doi = "10.5937/bnhmb2215171T",
pages = "171-189"
}

Tomović, L., Vučić, T., Anđelković, M., Urošević, A., Bjelica, V., Maričić, M., Lakušić, M., Danon, G.,& Ivanović, A.. (2022). Contribution to knowledge of batracho- and herpetofauna of southern and south-eastern Serbia. in Bulletin of the Natural History Museum
Belgrade: Natural History Museum., 15, 171-189.
https://doi.org/10.5937/bnhmb2215171T

Tomović L, Vučić T, Anđelković M, Urošević A, Bjelica V, Maričić M, Lakušić M, Danon G, Ivanović A. Contribution to knowledge of batracho- and herpetofauna of southern and south-eastern Serbia. in Bulletin of the Natural History Museum. 2022;15:171-189.
doi:10.5937/bnhmb2215171T .

Tomović, Ljiljana, Vučić, Tijana, Anđelković, Marko, Urošević, Aleksandar, Bjelica, Vukašin, Maričić, Marko, Lakušić, Margareta, Danon, Gorana, Ivanović, Ana, "Contribution to knowledge of batracho- and herpetofauna of southern and south-eastern Serbia" in Bulletin of the Natural History Museum, 15 (2022):171-189,
https://doi.org/10.5937/bnhmb2215171T . .

TY  - JOUR
AU  - Urošević, Aleksandar
AU  - Crnobrnja-Isailović, Jelka
AU  - Ljubisavljević, Katarina
AU  - Vukov, Tanja
AU  - Anđelković, Marko
AU  - Ivanović, Ana
AU  - Golubović, Ana
AU  - Vučić, Tijana
AU  - Tomović, Ljiljana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5549
AB  - In this paper, the updated checklist of Serbian amphibians and reptiles is
presented. The updated checklist of Serbian amphibians and reptiles was compiled
from the literature on distribution, taxonomy and phylogeny. The checklist
contains 48 autochthonous and two introduced species. Five species underwent
taxonomic revisions (Triturus ivanbureschi, T. macedonicus, Pelobates balcanicus,
Bufotes viridis and Mediodactylus kotschyi). Two species were added after recent
taxonomic splits of species’ complexes (Hyla orientalis and Anguis colchica).
Presence of new (Lacerta trilineata) and introduced species (Hemidactylus turcicus)
was confirmed during recent fieldwork. There are at least eight more species
that can potentially be present in Serbia. The national protection legislative should
be updated according to the current checklist.
AB  - У овом раду приказујемо ажурирану листу фауне водоземаца и
гмизаваца у Србији. Листа је састављена на основу литературе о
дистрибуцији, таксономији и филогенији водоземаца и гмизаваца који
припадају нашој фауни. Садржи 48 врста аутохтоних за Републику
Србију као и две интродуковане врсте за које је потврђено да се на
територији Србије размножавају и успостављају популације. Пет
врста на овој листи су недавно прошле кроз таксономску ревизију
(Triturus ivanbureschi, T. macedonicus, Pelobates balcanicus, Bufotes
viridis и Mediodactylus kotschyi), а две врсте су придодате након
скорашњег таксономског раздвајања комплекса врста (Hyla orientalis и
Anguis colchica). Најновија теренска истраживања су потврдила при-
суство једне нове (Lacerta trilineata) и једне интродуковане врсте
(Hemidactylus turcicus). Потенцијално је на територији Репубике Срби-
је присутно још најмање осам врста водоземаца и гмизаваца, пре свега
у пограничним областима. Од 48 врста аутохтоних за Републику
Србију, седам су рањиве/готово угрожене на међународном нивоу,
према критеријумима IUCN, три су у прилозима CITES-а, 39 се налазе
на анексима Директиве о стаништима (Habitat Directive), а све су на
прилозима Бернске конвенције. У току је процена конзервационог
статуса свих врста на националном нивоу, на основу чега ће бити
предложена нова легислатива.
Са 48 врста, Србија се може сматрати за богату биолошком
разноврсношћу водоземаца и гмизаваца па чак и центром диверзитета
појединих таксона – пре свега великих мрмољака и других комплекса
врста (Hyla, Pelophylax, Anguis). Даља истраживања батрахо- и херпе-
тофауне требало би концентрисати на контактне/хибридне зоне
комплекса врста и пограничне области наше земље.
PB  - Belgrade: Natural History Museum
T2  - Bulletin of the Natural History Museum
T1  - An updated checklist of the Serbian batracho- and herpetofauna
T1  - Ажурирана листа батрахо- и херпетофауне Републике Србије
VL  - 15
DO  - 10.5937/bnhmb2215149U
SP  - 149
EP  - 169
ER  - 

@article{
author = "Urošević, Aleksandar and Crnobrnja-Isailović, Jelka and Ljubisavljević, Katarina and Vukov, Tanja and Anđelković, Marko and Ivanović, Ana and Golubović, Ana and Vučić, Tijana and Tomović, Ljiljana",
year = "2022",
abstract = "In this paper, the updated checklist of Serbian amphibians and reptiles is
presented. The updated checklist of Serbian amphibians and reptiles was compiled
from the literature on distribution, taxonomy and phylogeny. The checklist
contains 48 autochthonous and two introduced species. Five species underwent
taxonomic revisions (Triturus ivanbureschi, T. macedonicus, Pelobates balcanicus,
Bufotes viridis and Mediodactylus kotschyi). Two species were added after recent
taxonomic splits of species’ complexes (Hyla orientalis and Anguis colchica).
Presence of new (Lacerta trilineata) and introduced species (Hemidactylus turcicus)
was confirmed during recent fieldwork. There are at least eight more species
that can potentially be present in Serbia. The national protection legislative should
be updated according to the current checklist., У овом раду приказујемо ажурирану листу фауне водоземаца и
гмизаваца у Србији. Листа је састављена на основу литературе о
дистрибуцији, таксономији и филогенији водоземаца и гмизаваца који
припадају нашој фауни. Садржи 48 врста аутохтоних за Републику
Србију као и две интродуковане врсте за које је потврђено да се на
територији Србије размножавају и успостављају популације. Пет
врста на овој листи су недавно прошле кроз таксономску ревизију
(Triturus ivanbureschi, T. macedonicus, Pelobates balcanicus, Bufotes
viridis и Mediodactylus kotschyi), а две врсте су придодате након
скорашњег таксономског раздвајања комплекса врста (Hyla orientalis и
Anguis colchica). Најновија теренска истраживања су потврдила при-
суство једне нове (Lacerta trilineata) и једне интродуковане врсте
(Hemidactylus turcicus). Потенцијално је на територији Репубике Срби-
је присутно још најмање осам врста водоземаца и гмизаваца, пре свега
у пограничним областима. Од 48 врста аутохтоних за Републику
Србију, седам су рањиве/готово угрожене на међународном нивоу,
према критеријумима IUCN, три су у прилозима CITES-а, 39 се налазе
на анексима Директиве о стаништима (Habitat Directive), а све су на
прилозима Бернске конвенције. У току је процена конзервационог
статуса свих врста на националном нивоу, на основу чега ће бити
предложена нова легислатива.
Са 48 врста, Србија се може сматрати за богату биолошком
разноврсношћу водоземаца и гмизаваца па чак и центром диверзитета
појединих таксона – пре свега великих мрмољака и других комплекса
врста (Hyla, Pelophylax, Anguis). Даља истраживања батрахо- и херпе-
тофауне требало би концентрисати на контактне/хибридне зоне
комплекса врста и пограничне области наше земље.",
publisher = "Belgrade: Natural History Museum",
journal = "Bulletin of the Natural History Museum",
title = "An updated checklist of the Serbian batracho- and herpetofauna, Ажурирана листа батрахо- и херпетофауне Републике Србије",
volume = "15",
doi = "10.5937/bnhmb2215149U",
pages = "149-169"
}

Urošević, A., Crnobrnja-Isailović, J., Ljubisavljević, K., Vukov, T., Anđelković, M., Ivanović, A., Golubović, A., Vučić, T.,& Tomović, L.. (2022). An updated checklist of the Serbian batracho- and herpetofauna. in Bulletin of the Natural History Museum
Belgrade: Natural History Museum., 15, 149-169.
https://doi.org/10.5937/bnhmb2215149U

Urošević A, Crnobrnja-Isailović J, Ljubisavljević K, Vukov T, Anđelković M, Ivanović A, Golubović A, Vučić T, Tomović L. An updated checklist of the Serbian batracho- and herpetofauna. in Bulletin of the Natural History Museum. 2022;15:149-169.
doi:10.5937/bnhmb2215149U .

Urošević, Aleksandar, Crnobrnja-Isailović, Jelka, Ljubisavljević, Katarina, Vukov, Tanja, Anđelković, Marko, Ivanović, Ana, Golubović, Ana, Vučić, Tijana, Tomović, Ljiljana, "An updated checklist of the Serbian batracho- and herpetofauna" in Bulletin of the Natural History Museum, 15 (2022):149-169,
https://doi.org/10.5937/bnhmb2215149U . .

TY  - JOUR
AU  - Urošević, Aleksandar
AU  - Anđelković, Marko
AU  - Crnobrnja-Isailović, Jelka
AU  - Krizmanić, Imre
AU  - Ajtić, Rastko
AU  - Simović, Aleksandar
AU  - Krstić, Milivoj
AU  - Maričić, Marko
AU  - Vučić, Tijana
AU  - Jović, Danko
AU  - Džukić, Georg
AU  - Tomović, Ljiljana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5550
AB  - We present the updated distribution data of the Hyla arborea species complex
in Serbia. Hyla arborea inhabits all biogeographic regions, while Hyla orientalis
inhabits only the easternmost parts of north-eastern, eastern and south-eastern
Serbia. Potential hybrid zone encompasses parts of central and southern Serbia
from the Đerdap gorge, over Niš to Bosilegrad. Since the taxonomic separation between the tree frog taxa was not yet recognized in national nomenclature, we
propose the official Serbian names. The species H. arborea will keep the standard
Serbian name “Gatalinka”, while for the species H. orientalis we propose the name
“Istočna gatalinka”.
AB  - У овом раду, приказујемо ажуриране податке о дистрибуцији
комплекса врста Hyla arborea у Србији. Подаци се састоје од налаза
објављених у литератури или на Интернету, заједно са претходно
необјављеним теренским подацима. Од ове две врсте, највећи део
територије Србије, укључујући све биогеографске регионе, насељава
H. arborea. Hyla orientalis, са друге стране, насељава само крајње
источне делове североисточне, источне и југоисточне Србије, са
потенцијалном хибридном зоном која такође обухвата делове централ-
не и јужне Србије. Дистрибуција H. arborea је густа у панонским
деловима Србије, конкретно у плавним зонама река Дунав, Сава и
Тиса, док је дистрибуција више раштркана у перипанонским и
планинско-котлинским деловима Србије. Hyla orientalis насељава
искључиво најисточније делове планинско-котлинског региона, где је
повезана са воденим системима који припадају Балканском планин-
ском венцу, док Карпатски и Родопски масиви представљају северо-
источну и југоисточну границу дистрибуције, респективно. За хибрид-
ну зону се претпоставља да обухвата оквирно 30 км широк коридор
који се протеже од Ђердапске клисуре, преко Ниша до Босилеграда,
али додатно узорковање ће морати да се изврши како би се ово
потврдило.
Пошто таксономско раздвајање између криптичних врста гаталин-
ки још увек није препознато у нашој националној номенклатури, овај
рад предлаже званичне српске називе за ове врсте. Номинална врста
H. arborea ће задржати стандардни српски назив „гаталинка“, док за
врсту H. orientalis предлажемо име „источна гаталинка“.
PB  - Belgrade: Natural History Museum
T2  - Bulletin of the Natural History Museum
T1  - Distribution of tree frogs (Hyla spp.) in Serbia – implications of the recent taxonomic revision
T1  - Дистрибуција гаталинки (Hyla spp .) у Србији – импликације скорашње таксономске ревизије
VL  - 15
DO  - 10.5937/bnhmb2215137U
SP  - 137
EP  - 148
ER  - 

@article{
author = "Urošević, Aleksandar and Anđelković, Marko and Crnobrnja-Isailović, Jelka and Krizmanić, Imre and Ajtić, Rastko and Simović, Aleksandar and Krstić, Milivoj and Maričić, Marko and Vučić, Tijana and Jović, Danko and Džukić, Georg and Tomović, Ljiljana",
year = "2022",
abstract = "We present the updated distribution data of the Hyla arborea species complex
in Serbia. Hyla arborea inhabits all biogeographic regions, while Hyla orientalis
inhabits only the easternmost parts of north-eastern, eastern and south-eastern
Serbia. Potential hybrid zone encompasses parts of central and southern Serbia
from the Đerdap gorge, over Niš to Bosilegrad. Since the taxonomic separation between the tree frog taxa was not yet recognized in national nomenclature, we
propose the official Serbian names. The species H. arborea will keep the standard
Serbian name “Gatalinka”, while for the species H. orientalis we propose the name
“Istočna gatalinka”., У овом раду, приказујемо ажуриране податке о дистрибуцији
комплекса врста Hyla arborea у Србији. Подаци се састоје од налаза
објављених у литератури или на Интернету, заједно са претходно
необјављеним теренским подацима. Од ове две врсте, највећи део
територије Србије, укључујући све биогеографске регионе, насељава
H. arborea. Hyla orientalis, са друге стране, насељава само крајње
источне делове североисточне, источне и југоисточне Србије, са
потенцијалном хибридном зоном која такође обухвата делове централ-
не и јужне Србије. Дистрибуција H. arborea је густа у панонским
деловима Србије, конкретно у плавним зонама река Дунав, Сава и
Тиса, док је дистрибуција више раштркана у перипанонским и
планинско-котлинским деловима Србије. Hyla orientalis насељава
искључиво најисточније делове планинско-котлинског региона, где је
повезана са воденим системима који припадају Балканском планин-
ском венцу, док Карпатски и Родопски масиви представљају северо-
источну и југоисточну границу дистрибуције, респективно. За хибрид-
ну зону се претпоставља да обухвата оквирно 30 км широк коридор
који се протеже од Ђердапске клисуре, преко Ниша до Босилеграда,
али додатно узорковање ће морати да се изврши како би се ово
потврдило.
Пошто таксономско раздвајање између криптичних врста гаталин-
ки још увек није препознато у нашој националној номенклатури, овај
рад предлаже званичне српске називе за ове врсте. Номинална врста
H. arborea ће задржати стандардни српски назив „гаталинка“, док за
врсту H. orientalis предлажемо име „источна гаталинка“.",
publisher = "Belgrade: Natural History Museum",
journal = "Bulletin of the Natural History Museum",
title = "Distribution of tree frogs (Hyla spp.) in Serbia – implications of the recent taxonomic revision, Дистрибуција гаталинки (Hyla spp .) у Србији – импликације скорашње таксономске ревизије",
volume = "15",
doi = "10.5937/bnhmb2215137U",
pages = "137-148"
}

Urošević, A., Anđelković, M., Crnobrnja-Isailović, J., Krizmanić, I., Ajtić, R., Simović, A., Krstić, M., Maričić, M., Vučić, T., Jović, D., Džukić, G.,& Tomović, L.. (2022). Distribution of tree frogs (Hyla spp.) in Serbia – implications of the recent taxonomic revision. in Bulletin of the Natural History Museum
Belgrade: Natural History Museum., 15, 137-148.
https://doi.org/10.5937/bnhmb2215137U

Urošević A, Anđelković M, Crnobrnja-Isailović J, Krizmanić I, Ajtić R, Simović A, Krstić M, Maričić M, Vučić T, Jović D, Džukić G, Tomović L. Distribution of tree frogs (Hyla spp.) in Serbia – implications of the recent taxonomic revision. in Bulletin of the Natural History Museum. 2022;15:137-148.
doi:10.5937/bnhmb2215137U .

Urošević, Aleksandar, Anđelković, Marko, Crnobrnja-Isailović, Jelka, Krizmanić, Imre, Ajtić, Rastko, Simović, Aleksandar, Krstić, Milivoj, Maričić, Marko, Vučić, Tijana, Jović, Danko, Džukić, Georg, Tomović, Ljiljana, "Distribution of tree frogs (Hyla spp.) in Serbia – implications of the recent taxonomic revision" in Bulletin of the Natural History Museum, 15 (2022):137-148,
https://doi.org/10.5937/bnhmb2215137U . .

TY  - CONF
AU  - Vučić, Tijana
AU  - Ivanović, Ana
AU  - Cvijanović, Milena
AU  - Wielstra, Ben
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5588
AB  - Large-bodied newts (Triturus sp.) represent a good model system for evo-devo studies. Females lay relatively large eggs, which are protected by transparent jelly layer, enabling easily observation of external anatomical features throughout embryonic development – from fertilized egg to fully formed larvae. MicroCT scanning aids another, more in depth insight into the development. Virtual cross sections enable visualization of internal anatomy, while volume renderings and reconstructions of 3D models enable exploration of development of internal organs and external morphology. Throughout Triturus embryonic development, homozygous embryos die due to a balanced lethal system known as chromosome 1 syndrome, which involves two non-recombining types of chromosome 1 (1A and 1B). It is hypothesized that the two potential homozygous genotypes (1A1A and 1B1B) arrest at different embryonic stages and express different phenotypes. Hence, knowledge of the embryo’s genotype is crucial for understanding normal embryonic development of heterozygous embryos and malformations which lead to lethality in homozygous embryos. In a pilot project, we tested and optimized a protocol for Triturus embryo scanning which allowed exploration of external and internal morphology while preserving the DNA. Our protocol provides an opportunity to further explore embryonic development in Triturus in diverse research lines: 1) normal embryonic development as a base line for further studies; 2) arrested development to uncover the basis of lethality; and 3) testing various evo-devo hypotheses.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
T1  - Insights from the inside: 3D embryonic development of Triturus newts
SP  - 40
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5588
ER  - 

@conference{
author = "Vučić, Tijana and Ivanović, Ana and Cvijanović, Milena and Wielstra, Ben",
year = "2022",
abstract = "Large-bodied newts (Triturus sp.) represent a good model system for evo-devo studies. Females lay relatively large eggs, which are protected by transparent jelly layer, enabling easily observation of external anatomical features throughout embryonic development – from fertilized egg to fully formed larvae. MicroCT scanning aids another, more in depth insight into the development. Virtual cross sections enable visualization of internal anatomy, while volume renderings and reconstructions of 3D models enable exploration of development of internal organs and external morphology. Throughout Triturus embryonic development, homozygous embryos die due to a balanced lethal system known as chromosome 1 syndrome, which involves two non-recombining types of chromosome 1 (1A and 1B). It is hypothesized that the two potential homozygous genotypes (1A1A and 1B1B) arrest at different embryonic stages and express different phenotypes. Hence, knowledge of the embryo’s genotype is crucial for understanding normal embryonic development of heterozygous embryos and malformations which lead to lethality in homozygous embryos. In a pilot project, we tested and optimized a protocol for Triturus embryo scanning which allowed exploration of external and internal morphology while preserving the DNA. Our protocol provides an opportunity to further explore embryonic development in Triturus in diverse research lines: 1) normal embryonic development as a base line for further studies; 2) arrested development to uncover the basis of lethality; and 3) testing various evo-devo hypotheses.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia",
title = "Insights from the inside: 3D embryonic development of Triturus newts",
pages = "40",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5588"
}

Vučić, T., Ivanović, A., Cvijanović, M.,& Wielstra, B.. (2022). Insights from the inside: 3D embryonic development of Triturus newts. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 40.
https://hdl.handle.net/21.15107/rcub_ibiss_5588

Vučić T, Ivanović A, Cvijanović M, Wielstra B. Insights from the inside: 3D embryonic development of Triturus newts. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. 2022;:40.
https://hdl.handle.net/21.15107/rcub_ibiss_5588 .

Vučić, Tijana, Ivanović, Ana, Cvijanović, Milena, Wielstra, Ben, "Insights from the inside: 3D embryonic development of Triturus newts" in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia (2022):40,
https://hdl.handle.net/21.15107/rcub_ibiss_5588 .

TY  - CONF
AU  - Tomović, Ljiljana
AU  - Vučić, Tijana
AU  - Anđelković, Marko
AU  - Bjelica, Vukašin
AU  - Maričić, Marko
AU  - Lakušić, Margareta
AU  - Danon, Gorana
AU  - Urošević, Aleksandar
AU  - Ivanović, Ana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5272
AB  - The establishment of ecological networks (including Natura 2000) is one of the priorities in the conservation of important species and habitats in the Republic of Serbia. During the last three years (2020-2022), we conducted field research on amphibian and reptile species listed in the Annexes II & IV of the Habitat Directive. The main goals were to obtain data about precise geographic distribution, estimate population statuses and conservation threats, as well as to propose conservation measures for the studied populations and their habitats. Field studies lasted more than 70 days and included over 60 locations across the country. Focal groups were species listed in Annex II: Triturus cristatus, T. dobrogicus, T. ivanbureschi, T. macedonicus, Testudo graeca, T. hermanni, Emys orbicularis, Elaphe quatuorlineata and Vipera ursinii. Results of these studies provided a more precise geographic distribution of crested newts and their hybrid zones in Serbia. For two species, we proposed the protection of two locations at the national level: one in eastern Serbia (for T. ivanbureschi) and one in southern Serbia (for T. macedonicus). The most threatened regions, where massive habitat losses and changes were detected, are the valleys of Velika and Zapadna Morava rivers, as well as wetlands along the Danube and Sava rivers. Populations of crested newts in Serbia are endangered by several threatening factors: habitat destructions/alterations, agriculture, water pollution and climatic changes. For reptiles, the most important locations for conservation of species listed in Annex II were detected in southeastern, southern and southwestern Serbia. For three reptile species with limited distribution and small populations, we proposed two locations in southern and southwestern Serbia for protection at the national level. The main threats for those species are habitat destruction/alternation and climatic changes. Results of these studies pointed out inconsistencies in conservation priorities between the national and European levels.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
T1  - Establishment of ecological networks in Serbia – field research of batracho- and herpetofauna
SP  - 71
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5272
ER  - 

@conference{
author = "Tomović, Ljiljana and Vučić, Tijana and Anđelković, Marko and Bjelica, Vukašin and Maričić, Marko and Lakušić, Margareta and Danon, Gorana and Urošević, Aleksandar and Ivanović, Ana",
year = "2022",
abstract = "The establishment of ecological networks (including Natura 2000) is one of the priorities in the conservation of important species and habitats in the Republic of Serbia. During the last three years (2020-2022), we conducted field research on amphibian and reptile species listed in the Annexes II & IV of the Habitat Directive. The main goals were to obtain data about precise geographic distribution, estimate population statuses and conservation threats, as well as to propose conservation measures for the studied populations and their habitats. Field studies lasted more than 70 days and included over 60 locations across the country. Focal groups were species listed in Annex II: Triturus cristatus, T. dobrogicus, T. ivanbureschi, T. macedonicus, Testudo graeca, T. hermanni, Emys orbicularis, Elaphe quatuorlineata and Vipera ursinii. Results of these studies provided a more precise geographic distribution of crested newts and their hybrid zones in Serbia. For two species, we proposed the protection of two locations at the national level: one in eastern Serbia (for T. ivanbureschi) and one in southern Serbia (for T. macedonicus). The most threatened regions, where massive habitat losses and changes were detected, are the valleys of Velika and Zapadna Morava rivers, as well as wetlands along the Danube and Sava rivers. Populations of crested newts in Serbia are endangered by several threatening factors: habitat destructions/alterations, agriculture, water pollution and climatic changes. For reptiles, the most important locations for conservation of species listed in Annex II were detected in southeastern, southern and southwestern Serbia. For three reptile species with limited distribution and small populations, we proposed two locations in southern and southwestern Serbia for protection at the national level. The main threats for those species are habitat destruction/alternation and climatic changes. Results of these studies pointed out inconsistencies in conservation priorities between the national and European levels.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia",
title = "Establishment of ecological networks in Serbia – field research of batracho- and herpetofauna",
pages = "71",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5272"
}

Tomović, L., Vučić, T., Anđelković, M., Bjelica, V., Maričić, M., Lakušić, M., Danon, G., Urošević, A.,& Ivanović, A.. (2022). Establishment of ecological networks in Serbia – field research of batracho- and herpetofauna. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 71.
https://hdl.handle.net/21.15107/rcub_ibiss_5272

Tomović L, Vučić T, Anđelković M, Bjelica V, Maričić M, Lakušić M, Danon G, Urošević A, Ivanović A. Establishment of ecological networks in Serbia – field research of batracho- and herpetofauna. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. 2022;:71.
https://hdl.handle.net/21.15107/rcub_ibiss_5272 .

Tomović, Ljiljana, Vučić, Tijana, Anđelković, Marko, Bjelica, Vukašin, Maričić, Marko, Lakušić, Margareta, Danon, Gorana, Urošević, Aleksandar, Ivanović, Ana, "Establishment of ecological networks in Serbia – field research of batracho- and herpetofauna" in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia (2022):71,
https://hdl.handle.net/21.15107/rcub_ibiss_5272 .

TY  - CONF
AU  - Urošević, Aleksandar
AU  - Ajduković, Maja
AU  - Vučić, Tijana
AU  - Scholtes, Stefan
AU  - Arntzen, Jan
AU  - Ivanović, Ana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5231
AB  - Serially homologous structures, such as the vertebral column, often undergo 
functional and evolutionary diversification and are a good model-system for studies of 
regionalization and morphological integration. We studied these topics in the vertebral 
column (atlas, trunk and sacral vertebrae) of the closely related taxa of small-bodied 
newts – Lissotriton schmidtleri, L. vulgaris ampelensis, L. v. meridionalis and L. v. 
vulgaris, using 3D geometric morphometrics on models that were acquired with micro CT scanning. Two different statistical approaches were employed to test for vertebral 
regionalization and overall morphological integration, namely segmented linear 
regression (SLR) and a partial least squares method (PLS) We observed a common 
pattern of regionalization, with a transition point after the 5th trunk vertebra. It 
corresponds with the antero-posterior transition common for tetrapods. 
Morphological integration, accessed via PLS analysis, is strongest at the 6th and 7th
trunk vertebrae, while the anterior and distal parts of the vertebral column are less 
integrated. The PLS analysis of the asymmetric component of shape variation revealed 
a weak integration, statistically significant only among subsequent trunk vertebrae. In 
summary, the vertebral column of the closely related Lissotriton newts is subtly 
regionalized, while being morphologically integrated overall. There is a complex 
relationship between regionalization and morphological integration of the vertebral 
column, most likely influenced by the newt’s bi-phasic life cycle that instigates different 
functional constraints in the aquatic and terrestrial life stages
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
T1  - Vertebral regionalization vs. morphological integration in Lissotriton newts
SP  - 38
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5231
ER  - 

@conference{
author = "Urošević, Aleksandar and Ajduković, Maja and Vučić, Tijana and Scholtes, Stefan and Arntzen, Jan and Ivanović, Ana",
year = "2022",
abstract = "Serially homologous structures, such as the vertebral column, often undergo 
functional and evolutionary diversification and are a good model-system for studies of 
regionalization and morphological integration. We studied these topics in the vertebral 
column (atlas, trunk and sacral vertebrae) of the closely related taxa of small-bodied 
newts – Lissotriton schmidtleri, L. vulgaris ampelensis, L. v. meridionalis and L. v. 
vulgaris, using 3D geometric morphometrics on models that were acquired with micro CT scanning. Two different statistical approaches were employed to test for vertebral 
regionalization and overall morphological integration, namely segmented linear 
regression (SLR) and a partial least squares method (PLS) We observed a common 
pattern of regionalization, with a transition point after the 5th trunk vertebra. It 
corresponds with the antero-posterior transition common for tetrapods. 
Morphological integration, accessed via PLS analysis, is strongest at the 6th and 7th
trunk vertebrae, while the anterior and distal parts of the vertebral column are less 
integrated. The PLS analysis of the asymmetric component of shape variation revealed 
a weak integration, statistically significant only among subsequent trunk vertebrae. In 
summary, the vertebral column of the closely related Lissotriton newts is subtly 
regionalized, while being morphologically integrated overall. There is a complex 
relationship between regionalization and morphological integration of the vertebral 
column, most likely influenced by the newt’s bi-phasic life cycle that instigates different 
functional constraints in the aquatic and terrestrial life stages",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia",
title = "Vertebral regionalization vs. morphological integration in Lissotriton newts",
pages = "38",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5231"
}

Urošević, A., Ajduković, M., Vučić, T., Scholtes, S., Arntzen, J.,& Ivanović, A.. (2022). Vertebral regionalization vs. morphological integration in Lissotriton newts. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 38.
https://hdl.handle.net/21.15107/rcub_ibiss_5231

Urošević A, Ajduković M, Vučić T, Scholtes S, Arntzen J, Ivanović A. Vertebral regionalization vs. morphological integration in Lissotriton newts. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. 2022;:38.
https://hdl.handle.net/21.15107/rcub_ibiss_5231 .

Urošević, Aleksandar, Ajduković, Maja, Vučić, Tijana, Scholtes, Stefan, Arntzen, Jan, Ivanović, Ana, "Vertebral regionalization vs. morphological integration in Lissotriton newts" in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia (2022):38,
https://hdl.handle.net/21.15107/rcub_ibiss_5231 .

TY  - CONF
AU  - Drobnjaković, Marija
AU  - Ivanović, Ana
AU  - Bugarčić, Marko
AU  - Cvijanović, Milena
AU  - Ajduković, Maja
AU  - Vučić, Tijana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5230
AB  - Staging tables based on the external morphological characteristics are important 
for the needs of comparative embryological studies as they represent a baseline. Large bodied newts (Triturus spp.) have a transparent mucoid capsule that enables insight 
into the external morphology of the embryo during development. Triturus newts are 
characterized by the presence of chromosome 1 syndrome where about 50% of the 
offspring stops developing and dies during the mid embryonic period on the tail bud 
stage. In this study, we focused on the normal development of Balkan crested newt 
(Triturus ivanbureschi). During the breeding season animals were kept in the yard of 
the institute, in plastic tubs (from 200 to 400 liters), covered with a mosquito net that 
allowed natural day-night regime. Plastic strips were provided as an imitation of 
underwater vegetation, on which the females laid their eggs. Eggs were collected every 
morning and moved to Petri dishes at the controlled experimental conditions. The 
embryos were kept in the laboratory at a temperature of 20°C. Photographs and time lapse recordings of developing embryos were taken every day at the same time. 
Compared to already published staging tables for newts and based on the appearance 
of certain morphological structures, such as gills, extremities, balancers, etc., we
distinguished 37 different stages of normal embryonic development of T. ivanbureshi. 
Still, differences in external morphology due to the balanced lethal chromosome 1 
syndrome need further research
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
T1  - Developmental stages of Triturus ivanbureschi
VL  - 260
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5230
ER  - 

@conference{
author = "Drobnjaković, Marija and Ivanović, Ana and Bugarčić, Marko and Cvijanović, Milena and Ajduković, Maja and Vučić, Tijana",
year = "2022",
abstract = "Staging tables based on the external morphological characteristics are important 
for the needs of comparative embryological studies as they represent a baseline. Large bodied newts (Triturus spp.) have a transparent mucoid capsule that enables insight 
into the external morphology of the embryo during development. Triturus newts are 
characterized by the presence of chromosome 1 syndrome where about 50% of the 
offspring stops developing and dies during the mid embryonic period on the tail bud 
stage. In this study, we focused on the normal development of Balkan crested newt 
(Triturus ivanbureschi). During the breeding season animals were kept in the yard of 
the institute, in plastic tubs (from 200 to 400 liters), covered with a mosquito net that 
allowed natural day-night regime. Plastic strips were provided as an imitation of 
underwater vegetation, on which the females laid their eggs. Eggs were collected every 
morning and moved to Petri dishes at the controlled experimental conditions. The 
embryos were kept in the laboratory at a temperature of 20°C. Photographs and time lapse recordings of developing embryos were taken every day at the same time. 
Compared to already published staging tables for newts and based on the appearance 
of certain morphological structures, such as gills, extremities, balancers, etc., we
distinguished 37 different stages of normal embryonic development of T. ivanbureshi. 
Still, differences in external morphology due to the balanced lethal chromosome 1 
syndrome need further research",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia",
title = "Developmental stages of Triturus ivanbureschi",
volume = "260",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5230"
}

Drobnjaković, M., Ivanović, A., Bugarčić, M., Cvijanović, M., Ajduković, M.,& Vučić, T.. (2022). Developmental stages of Triturus ivanbureschi. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 260.
https://hdl.handle.net/21.15107/rcub_ibiss_5230

Drobnjaković M, Ivanović A, Bugarčić M, Cvijanović M, Ajduković M, Vučić T. Developmental stages of Triturus ivanbureschi. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. 2022;260.
https://hdl.handle.net/21.15107/rcub_ibiss_5230 .

Drobnjaković, Marija, Ivanović, Ana, Bugarčić, Marko, Cvijanović, Milena, Ajduković, Maja, Vučić, Tijana, "Developmental stages of Triturus ivanbureschi" in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia, 260 (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_5230 .

TY  - CONF
AU  - Mirč, Marko
AU  - Anđelković, Marko
AU  - Urošević, Aleksandar
AU  - Cvijanović, Milena
AU  - Tomašević Kolarov, Nataša
AU  - Ajduković, Maja
AU  - Kijanović, Ana
AU  - Vučić, Tijana
AU  - Vukov, Tanja
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5229
AB  - National parks are, by definition, areas set aside for wildlife protection, but also 
human recreation. Sometimes, the development of tourism-related infrastructure and 
capacities or resource exploitation can impede the primary function of national parks 
– nature conservation. Baseline and trend monitoring are great tools to assess human 
pressure on nature. Amphibian and reptile species have small ranges and are 
vulnerable to changes in their environment which makes them great bioindicators. 
Here we present the results of five-year monitoring activities of amphibian and reptile 
populations at National Park „Kopaonik“, Serbia. Data on batracho- and herpetofauna 
of NP „Kopaonik“ were limited and outdated, so a detailed survey of species richness, 
habitat conditions, and population trends was greatly needed. Starting from 2018 to 
2022 we described the amphibian and reptile diversity of the national park and 
systematically monitored several habitats, dispersed throughout all three protection 
zones as well as outside protection zones, to assess the habitat quality and population 
trends of selected species. We had an opportunity to see the effect of the international 
travel ban (due to the Covid19) on batracho- and herpetofauna, when much more 
people spent their vacation at NP „Kopaonik“ than usually. Per our results so far, the 
richness of the amphibians and reptiles species of the NP „Kopanik“ is moderate (9 
amphibian and 12 reptile species). The population size of the selected lizard species is 
stable through the years regardless of the protection zone. Amphibian populations are 
also stable, as offsprings of the majority of the species were found every year. However 
human pressure on the amphibian and reptile species is high. Habitat degradation is 
present and some localities are at great risk to be lost
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
T1  - Five years of monitoring amphibian and reptile populations at National Park  „Kopaonik“, Serbia
SP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5229
ER  - 

@conference{
author = "Mirč, Marko and Anđelković, Marko and Urošević, Aleksandar and Cvijanović, Milena and Tomašević Kolarov, Nataša and Ajduković, Maja and Kijanović, Ana and Vučić, Tijana and Vukov, Tanja",
year = "2022",
abstract = "National parks are, by definition, areas set aside for wildlife protection, but also 
human recreation. Sometimes, the development of tourism-related infrastructure and 
capacities or resource exploitation can impede the primary function of national parks 
– nature conservation. Baseline and trend monitoring are great tools to assess human 
pressure on nature. Amphibian and reptile species have small ranges and are 
vulnerable to changes in their environment which makes them great bioindicators. 
Here we present the results of five-year monitoring activities of amphibian and reptile 
populations at National Park „Kopaonik“, Serbia. Data on batracho- and herpetofauna 
of NP „Kopaonik“ were limited and outdated, so a detailed survey of species richness, 
habitat conditions, and population trends was greatly needed. Starting from 2018 to 
2022 we described the amphibian and reptile diversity of the national park and 
systematically monitored several habitats, dispersed throughout all three protection 
zones as well as outside protection zones, to assess the habitat quality and population 
trends of selected species. We had an opportunity to see the effect of the international 
travel ban (due to the Covid19) on batracho- and herpetofauna, when much more 
people spent their vacation at NP „Kopaonik“ than usually. Per our results so far, the 
richness of the amphibians and reptiles species of the NP „Kopanik“ is moderate (9 
amphibian and 12 reptile species). The population size of the selected lizard species is 
stable through the years regardless of the protection zone. Amphibian populations are 
also stable, as offsprings of the majority of the species were found every year. However 
human pressure on the amphibian and reptile species is high. Habitat degradation is 
present and some localities are at great risk to be lost",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia",
title = "Five years of monitoring amphibian and reptile populations at National Park  „Kopaonik“, Serbia",
pages = "79",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5229"
}

Mirč, M., Anđelković, M., Urošević, A., Cvijanović, M., Tomašević Kolarov, N., Ajduković, M., Kijanović, A., Vučić, T.,& Vukov, T.. (2022). Five years of monitoring amphibian and reptile populations at National Park  „Kopaonik“, Serbia. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 79.
https://hdl.handle.net/21.15107/rcub_ibiss_5229

Mirč M, Anđelković M, Urošević A, Cvijanović M, Tomašević Kolarov N, Ajduković M, Kijanović A, Vučić T, Vukov T. Five years of monitoring amphibian and reptile populations at National Park  „Kopaonik“, Serbia. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. 2022;:79.
https://hdl.handle.net/21.15107/rcub_ibiss_5229 .

Mirč, Marko, Anđelković, Marko, Urošević, Aleksandar, Cvijanović, Milena, Tomašević Kolarov, Nataša, Ajduković, Maja, Kijanović, Ana, Vučić, Tijana, Vukov, Tanja, "Five years of monitoring amphibian and reptile populations at National Park  „Kopaonik“, Serbia" in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia (2022):79,
https://hdl.handle.net/21.15107/rcub_ibiss_5229 .

TY  - CONF
AU  - Bugarčić, Marko
AU  - Ivanović, Ana
AU  - Cvijanović, Milena
AU  - Ajduković, Maja
AU  - Wielstra, Ben
AU  - Vučić, Tijana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5228
AB  - In developmental biology a plethora of studies support the existence of a
conserved stage during the ontogeny of vertebrates – the pharyngula. The
developmental hourglass model predicts that the most conserved morphological
pattern occurs in the middle of embryonic development. We explored external
morphology of the craniofacial region of Triturus newts using 3D geometric
morphometrics. The craniofacial region was selected because it experiences
pleiotropic developmental constrains due to its major role in feeding, respiration and
the housing of substantial parts of nervous and sensory systems. We aimed to uncover
differences in variance between successive developmental stages, because it is
proposed that the more constrained stages should have the lowest variance. In
addition, we explored differences in the craniofacial shape between successive
developmental stages. Stages included start from the moment after the completion of
neurulation and span the mid-tailbud phase. The least amount of variance in shape was
recorded at stage 24 (according to D’Amen and colleagues). In general, this stage is
characterized by changes in overall head shape, the distinction of optical vesicles,
intensive somitogenesis and formation of the tailbud which overgrows the blastopore,
indicating that these processes could be highly constrained. Only significant
craniofacial shape change occurred between this and successive stage. The most
pronounced differences were due to growing of the head region and gill bud balancers.
Optic vesicles became concave due to inductive processes preceding eye formation.
These preliminary results indicate that stage 24 could be the most conserved one
during early ontogeny as it is preceded and followed by stages exhibiting more
variance in shape. Our results indicate that the craniofacial development represents a
suitable model system for testing the hourglass model of development. Larger sample
size, exploration of the entire embryonic development and ontogeny of additional newt
species is needed to confirm our preliminary findings.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
T1  - Craniofacial development of Triturus newts – a suitable model system for  testing the developmental hourglass model
SP  - 42
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5228
ER  - 

@conference{
author = "Bugarčić, Marko and Ivanović, Ana and Cvijanović, Milena and Ajduković, Maja and Wielstra, Ben and Vučić, Tijana",
year = "2022",
abstract = "In developmental biology a plethora of studies support the existence of a
conserved stage during the ontogeny of vertebrates – the pharyngula. The
developmental hourglass model predicts that the most conserved morphological
pattern occurs in the middle of embryonic development. We explored external
morphology of the craniofacial region of Triturus newts using 3D geometric
morphometrics. The craniofacial region was selected because it experiences
pleiotropic developmental constrains due to its major role in feeding, respiration and
the housing of substantial parts of nervous and sensory systems. We aimed to uncover
differences in variance between successive developmental stages, because it is
proposed that the more constrained stages should have the lowest variance. In
addition, we explored differences in the craniofacial shape between successive
developmental stages. Stages included start from the moment after the completion of
neurulation and span the mid-tailbud phase. The least amount of variance in shape was
recorded at stage 24 (according to D’Amen and colleagues). In general, this stage is
characterized by changes in overall head shape, the distinction of optical vesicles,
intensive somitogenesis and formation of the tailbud which overgrows the blastopore,
indicating that these processes could be highly constrained. Only significant
craniofacial shape change occurred between this and successive stage. The most
pronounced differences were due to growing of the head region and gill bud balancers.
Optic vesicles became concave due to inductive processes preceding eye formation.
These preliminary results indicate that stage 24 could be the most conserved one
during early ontogeny as it is preceded and followed by stages exhibiting more
variance in shape. Our results indicate that the craniofacial development represents a
suitable model system for testing the hourglass model of development. Larger sample
size, exploration of the entire embryonic development and ontogeny of additional newt
species is needed to confirm our preliminary findings.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia",
title = "Craniofacial development of Triturus newts – a suitable model system for  testing the developmental hourglass model",
pages = "42",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5228"
}

Bugarčić, M., Ivanović, A., Cvijanović, M., Ajduković, M., Wielstra, B.,& Vučić, T.. (2022). Craniofacial development of Triturus newts – a suitable model system for  testing the developmental hourglass model. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 42.
https://hdl.handle.net/21.15107/rcub_ibiss_5228

Bugarčić M, Ivanović A, Cvijanović M, Ajduković M, Wielstra B, Vučić T. Craniofacial development of Triturus newts – a suitable model system for  testing the developmental hourglass model. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. 2022;:42.
https://hdl.handle.net/21.15107/rcub_ibiss_5228 .

Bugarčić, Marko, Ivanović, Ana, Cvijanović, Milena, Ajduković, Maja, Wielstra, Ben, Vučić, Tijana, "Craniofacial development of Triturus newts – a suitable model system for  testing the developmental hourglass model" in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia (2022):42,
https://hdl.handle.net/21.15107/rcub_ibiss_5228 .

TY  - JOUR
AU  - Ajduković, Maja
AU  - Vučić, Tijana
AU  - Cvijanović, Milena
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4251
AB  - Background
In amphibians, thyroid hormone (TH) has a profound role in cranial development, especially in ossification of the late-appearing bones and remodeling of the skull. In the present study, we explored the influence of TH deficiency on bone ossification and resulting skull shape during the ontogeny of Triturus newt hybrid larvae obtained from interspecific crosses between T. ivanbureschi and T. macedonicus.

Methods
Larvae were treated with two concentrations of thiourea (an endocrine disruptor that chemically inhibits synthesis of TH) during the midlarval and late larval periods. Morphological differences of the cranium were assessed at the end of the midlarval period (ontogenetic stage 62) and the metamorphic stage after treatment during the late larval period.

Results
There was no difference in the ossification level and shape of the skull between the experimental groups (control and two treatment concentrations) at stage 62. During the late larval period and metamorphosis, TH deficit had a significant impact on the level of bone ossification and skull shape with no differences between the two treatment concentrations of thiourea. The most pronounced differences in bone development were: the palatopterygoid failed to disintegrate into the palatal and pterygoid portions, retardation was observed in development of the maxilla, nasal and prefrontal bones and larval organization of the vomer was retained in thiourea-treated larvae.

Conclusions
This implies that deficiency of TH caused retardation in development and arrested metamorphic cranium skeletal reorganization, which resulted in divergent cranial shape compared to the control group. Our results confirmed that skull remodeling and ossification of late-appearing bones is TH–dependent, as in other studied Urodela species. Also, our results indicate that TH plays an important role in the establishment of skull shape during the ontogeny of Triturus newts, especially during the late larval period and metamorphosis, when TH concentrations reach their maximum.
PB  - London: PEERJ INC
T2  - PeerJ
T1  - Effects of thiourea on the skull of Triturus newts during ontogeny
VL  - 9
DO  - 10.7717/peerj.11535
SP  - e11535
ER  - 

@article{
author = "Ajduković, Maja and Vučić, Tijana and Cvijanović, Milena",
year = "2021",
abstract = "Background
In amphibians, thyroid hormone (TH) has a profound role in cranial development, especially in ossification of the late-appearing bones and remodeling of the skull. In the present study, we explored the influence of TH deficiency on bone ossification and resulting skull shape during the ontogeny of Triturus newt hybrid larvae obtained from interspecific crosses between T. ivanbureschi and T. macedonicus.

Methods
Larvae were treated with two concentrations of thiourea (an endocrine disruptor that chemically inhibits synthesis of TH) during the midlarval and late larval periods. Morphological differences of the cranium were assessed at the end of the midlarval period (ontogenetic stage 62) and the metamorphic stage after treatment during the late larval period.

Results
There was no difference in the ossification level and shape of the skull between the experimental groups (control and two treatment concentrations) at stage 62. During the late larval period and metamorphosis, TH deficit had a significant impact on the level of bone ossification and skull shape with no differences between the two treatment concentrations of thiourea. The most pronounced differences in bone development were: the palatopterygoid failed to disintegrate into the palatal and pterygoid portions, retardation was observed in development of the maxilla, nasal and prefrontal bones and larval organization of the vomer was retained in thiourea-treated larvae.

Conclusions
This implies that deficiency of TH caused retardation in development and arrested metamorphic cranium skeletal reorganization, which resulted in divergent cranial shape compared to the control group. Our results confirmed that skull remodeling and ossification of late-appearing bones is TH–dependent, as in other studied Urodela species. Also, our results indicate that TH plays an important role in the establishment of skull shape during the ontogeny of Triturus newts, especially during the late larval period and metamorphosis, when TH concentrations reach their maximum.",
publisher = "London: PEERJ INC",
journal = "PeerJ",
title = "Effects of thiourea on the skull of Triturus newts during ontogeny",
volume = "9",
doi = "10.7717/peerj.11535",
pages = "e11535"
}

Ajduković, M., Vučić, T.,& Cvijanović, M.. (2021). Effects of thiourea on the skull of Triturus newts during ontogeny. in PeerJ
London: PEERJ INC., 9, e11535.
https://doi.org/10.7717/peerj.11535

Ajduković M, Vučić T, Cvijanović M. Effects of thiourea on the skull of Triturus newts during ontogeny. in PeerJ. 2021;9:e11535.
doi:10.7717/peerj.11535 .

Ajduković, Maja, Vučić, Tijana, Cvijanović, Milena, "Effects of thiourea on the skull of Triturus newts during ontogeny" in PeerJ, 9 (2021):e11535,
https://doi.org/10.7717/peerj.11535 . .

TY  - JOUR
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Petrović, Tamara
AU  - Ajduković, Maja
AU  - Vučić, Tijana
AU  - Cvijanović, Milena
AU  - Faggio, Caterina
AU  - Prokić, Marko
PY  - 2021
UR  - https://www.mdpi.com/1660-4601/18/18/9653
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8464833
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4483
AB  - Thiourea is an established disruptor of thyroid hormone synthesis and is frequently used as an inhibitor of metamorphosis. The changes caused by thiourea can affect processes associated with the oxidative status of individuals (metabolic rate, the HPI axis, antioxidant system). We investigated the parameters of oxidative stress in crested newt (Triturus spp.) larvae during normal development in late larval stage 62 and newly metamorphosed individuals, and during thiourea-stimulated metamorphosis arrest in individuals exposed to low (0.05%) and high (0.1%) concentrations of thiourea. Both groups of crested newts exposed to thiourea retained their larval characteristics until the end of the experiment. The low activities of antioxidant enzymes and the high lipid peroxidation level pointed to increased oxidative stress in larvae at the beginning of stage 62 as compared to fully metamorphosed individuals. The activities of catalase (CAT) and glutathione-S-transferase (GST) and the concentration of sulfhydryl (SH) groups were significantly lower in larvae reared in aqueous solutions containing thiourea than in newly metamorphosed individuals. The high thiourea concentration (0.1%) affected the antioxidative parameters to the extent that oxidative damage could not be avoided, contrary to a lower concentration. Our results provide a first insight into the physiological adaptations of crested newts during normal development and simulated metamorphosis arrest.
PB  - Basel: MDPI
T2  - International Journal of Environmental Research and Public Health
T1  - Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis.
IS  - 18
VL  - 18
DO  - 10.3390/ijerph18189653
SP  - 9653
ER  - 

@article{
author = "Gavrić, Jelena and Despotović, Svetlana and Gavrilović, Branka and Radovanović, Tijana and Petrović, Tamara and Ajduković, Maja and Vučić, Tijana and Cvijanović, Milena and Faggio, Caterina and Prokić, Marko",
year = "2021",
abstract = "Thiourea is an established disruptor of thyroid hormone synthesis and is frequently used as an inhibitor of metamorphosis. The changes caused by thiourea can affect processes associated with the oxidative status of individuals (metabolic rate, the HPI axis, antioxidant system). We investigated the parameters of oxidative stress in crested newt (Triturus spp.) larvae during normal development in late larval stage 62 and newly metamorphosed individuals, and during thiourea-stimulated metamorphosis arrest in individuals exposed to low (0.05%) and high (0.1%) concentrations of thiourea. Both groups of crested newts exposed to thiourea retained their larval characteristics until the end of the experiment. The low activities of antioxidant enzymes and the high lipid peroxidation level pointed to increased oxidative stress in larvae at the beginning of stage 62 as compared to fully metamorphosed individuals. The activities of catalase (CAT) and glutathione-S-transferase (GST) and the concentration of sulfhydryl (SH) groups were significantly lower in larvae reared in aqueous solutions containing thiourea than in newly metamorphosed individuals. The high thiourea concentration (0.1%) affected the antioxidative parameters to the extent that oxidative damage could not be avoided, contrary to a lower concentration. Our results provide a first insight into the physiological adaptations of crested newts during normal development and simulated metamorphosis arrest.",
publisher = "Basel: MDPI",
journal = "International Journal of Environmental Research and Public Health",
title = "Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis.",
number = "18",
volume = "18",
doi = "10.3390/ijerph18189653",
pages = "9653"
}

Gavrić, J., Despotović, S., Gavrilović, B., Radovanović, T., Petrović, T., Ajduković, M., Vučić, T., Cvijanović, M., Faggio, C.,& Prokić, M.. (2021). Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis.. in International Journal of Environmental Research and Public Health
Basel: MDPI., 18(18), 9653.
https://doi.org/10.3390/ijerph18189653

Gavrić J, Despotović S, Gavrilović B, Radovanović T, Petrović T, Ajduković M, Vučić T, Cvijanović M, Faggio C, Prokić M. Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis.. in International Journal of Environmental Research and Public Health. 2021;18(18):9653.
doi:10.3390/ijerph18189653 .

Gavrić, Jelena, Despotović, Svetlana, Gavrilović, Branka, Radovanović, Tijana, Petrović, Tamara, Ajduković, Maja, Vučić, Tijana, Cvijanović, Milena, Faggio, Caterina, Prokić, Marko, "Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis." in International Journal of Environmental Research and Public Health, 18, no. 18 (2021):9653,
https://doi.org/10.3390/ijerph18189653 . .

TY  - JOUR
AU  - Prokić, Marko
AU  - Petrović, Tamara
AU  - Despotović, Svetlana
AU  - Vučić, Tijana
AU  - Gavrić, Jelena
AU  - Radovanović, Tijana
AU  - Gavrilović, Branka
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S1095643320301720
UR  - https://radar.ibiss.bg.ac.rs/123456789/3902
UR  - https://radar.ibiss.bg.ac.rs/123456789/3903
AB  - In nature, animals often face periods without food caused by seasonal fluctuations and/or prey scarcity. An organism's physiological response to imposed energetic limitations is followed by changes in mitochondrial functioning (adjustment of energy metabolism) and a reduction of non-essential processes. However, this energy-saving strategy can have its costs. In this study, we examined oxidative stress as one of the possible physiological costs of short-term, two-week-long food deprivation on developing amphibian larvae of the crested newts Triturus macedonicus and Triturus ivanbureschi and their hybrids. We investigated whether this exogenous factor additionally affected the oxidative status (fitness-related trait) of hybrid individuals. The fasting treatment led to lower growth and a lower body mass and body condition index of individuals. The results revealed that the antioxidant system (AOS) of food-deprived larvae could not cope in a proper manner with reactive oxygen species production under limited energy availability, leading to higher lipid oxidative damage. The lowest AOS response was observed for H2O2 scavenging parameters (catalase, glutathione peroxidase, and total glutathione), which together with the elevated activity of superoxide dismutase suggested increased H2O2 concentrations. Comparison between parental species and their hybrids showed that hybrid individuals suffered greater oxidative damage (as demonstrated by higher concentrations of lipid peroxides), indicating that they were more susceptible to fasting-induced oxidative stress. Overall, this study illustrates that: (i) an oxidative event is one of the costs amphibian larvae face during short-term periods of fasting, (ii) hybrids are less capable of dealing with this stressful condition, which can lower their chances of survival in a changing environment.
PB  - Pergamon
T2  - Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
T1  - The effect of short-term fasting on the oxidative status of larvae of crested newt species and their hybrids
VL  - 251
DO  - 10.1016/j.cbpa.2020.110819
SP  - 110819
ER  - 

@article{
author = "Prokić, Marko and Petrović, Tamara and Despotović, Svetlana and Vučić, Tijana and Gavrić, Jelena and Radovanović, Tijana and Gavrilović, Branka",
year = "2021",
abstract = "In nature, animals often face periods without food caused by seasonal fluctuations and/or prey scarcity. An organism's physiological response to imposed energetic limitations is followed by changes in mitochondrial functioning (adjustment of energy metabolism) and a reduction of non-essential processes. However, this energy-saving strategy can have its costs. In this study, we examined oxidative stress as one of the possible physiological costs of short-term, two-week-long food deprivation on developing amphibian larvae of the crested newts Triturus macedonicus and Triturus ivanbureschi and their hybrids. We investigated whether this exogenous factor additionally affected the oxidative status (fitness-related trait) of hybrid individuals. The fasting treatment led to lower growth and a lower body mass and body condition index of individuals. The results revealed that the antioxidant system (AOS) of food-deprived larvae could not cope in a proper manner with reactive oxygen species production under limited energy availability, leading to higher lipid oxidative damage. The lowest AOS response was observed for H2O2 scavenging parameters (catalase, glutathione peroxidase, and total glutathione), which together with the elevated activity of superoxide dismutase suggested increased H2O2 concentrations. Comparison between parental species and their hybrids showed that hybrid individuals suffered greater oxidative damage (as demonstrated by higher concentrations of lipid peroxides), indicating that they were more susceptible to fasting-induced oxidative stress. Overall, this study illustrates that: (i) an oxidative event is one of the costs amphibian larvae face during short-term periods of fasting, (ii) hybrids are less capable of dealing with this stressful condition, which can lower their chances of survival in a changing environment.",
publisher = "Pergamon",
journal = "Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology",
title = "The effect of short-term fasting on the oxidative status of larvae of crested newt species and their hybrids",
volume = "251",
doi = "10.1016/j.cbpa.2020.110819",
pages = "110819"
}

Prokić, M., Petrović, T., Despotović, S., Vučić, T., Gavrić, J., Radovanović, T.,& Gavrilović, B.. (2021). The effect of short-term fasting on the oxidative status of larvae of crested newt species and their hybrids. in Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
Pergamon., 251, 110819.
https://doi.org/10.1016/j.cbpa.2020.110819

Prokić M, Petrović T, Despotović S, Vučić T, Gavrić J, Radovanović T, Gavrilović B. The effect of short-term fasting on the oxidative status of larvae of crested newt species and their hybrids. in Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. 2021;251:110819.
doi:10.1016/j.cbpa.2020.110819 .

Prokić, Marko, Petrović, Tamara, Despotović, Svetlana, Vučić, Tijana, Gavrić, Jelena, Radovanović, Tijana, Gavrilović, Branka, "The effect of short-term fasting on the oxidative status of larvae of crested newt species and their hybrids" in Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 251 (2021):110819,
https://doi.org/10.1016/j.cbpa.2020.110819 . .

TY  - JOUR
AU  - Prokić, Marko
AU  - Petrović, Tamara
AU  - Despotović, Svetlana
AU  - Vučić, Tijana
AU  - Gavrić, Jelena
AU  - Radovanović, Tijana
AU  - Gavrilović, Branka
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S1095643320301720
UR  - https://radar.ibiss.bg.ac.rs/123456789/3902
AB  - In nature, animals often face periods without food caused by seasonal fluctuations and/or prey scarcity. An organism's physiological response to imposed energetic limitations is followed by changes in mitochondrial functioning (adjustment of energy metabolism) and a reduction of non-essential processes. However, this energy-saving strategy can have its costs. In this study, we examined oxidative stress as one of the possible physiological costs of short-term, two-week-long food deprivation on developing amphibian larvae of the crested newts Triturus macedonicus and Triturus ivanbureschi and their hybrids. We investigated whether this exogenous factor additionally affected the oxidative status (fitness-related trait) of hybrid individuals. The fasting treatment led to lower growth and a lower body mass and body condition index of individuals. The results revealed that the antioxidant system (AOS) of food-deprived larvae could not cope in a proper manner with reactive oxygen species production under limited energy availability, leading to higher lipid oxidative damage. The lowest AOS response was observed for H2O2 scavenging parameters (catalase, glutathione peroxidase, and total glutathione), which together with the elevated activity of superoxide dismutase suggested increased H2O2 concentrations. Comparison between parental species and their hybrids showed that hybrid individuals suffered greater oxidative damage (as demonstrated by higher concentrations of lipid peroxides), indicating that they were more susceptible to fasting-induced oxidative stress. Overall, this study illustrates that: (i) an oxidative event is one of the costs amphibian larvae face during short-term periods of fasting, (ii) hybrids are less capable of dealing with this stressful condition, which can lower their chances of survival in a changing environment.
PB  - Pergamon
T2  - Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
T1  - The effect of short-term fasting on the oxidative status of larvae of crested newt species and their hybrids
VL  - 251
DO  - 10.1016/j.cbpa.2020.110819
SP  - 110819
ER  - 

@article{
author = "Prokić, Marko and Petrović, Tamara and Despotović, Svetlana and Vučić, Tijana and Gavrić, Jelena and Radovanović, Tijana and Gavrilović, Branka",
year = "2021",
abstract = "In nature, animals often face periods without food caused by seasonal fluctuations and/or prey scarcity. An organism's physiological response to imposed energetic limitations is followed by changes in mitochondrial functioning (adjustment of energy metabolism) and a reduction of non-essential processes. However, this energy-saving strategy can have its costs. In this study, we examined oxidative stress as one of the possible physiological costs of short-term, two-week-long food deprivation on developing amphibian larvae of the crested newts Triturus macedonicus and Triturus ivanbureschi and their hybrids. We investigated whether this exogenous factor additionally affected the oxidative status (fitness-related trait) of hybrid individuals. The fasting treatment led to lower growth and a lower body mass and body condition index of individuals. The results revealed that the antioxidant system (AOS) of food-deprived larvae could not cope in a proper manner with reactive oxygen species production under limited energy availability, leading to higher lipid oxidative damage. The lowest AOS response was observed for H2O2 scavenging parameters (catalase, glutathione peroxidase, and total glutathione), which together with the elevated activity of superoxide dismutase suggested increased H2O2 concentrations. Comparison between parental species and their hybrids showed that hybrid individuals suffered greater oxidative damage (as demonstrated by higher concentrations of lipid peroxides), indicating that they were more susceptible to fasting-induced oxidative stress. Overall, this study illustrates that: (i) an oxidative event is one of the costs amphibian larvae face during short-term periods of fasting, (ii) hybrids are less capable of dealing with this stressful condition, which can lower their chances of survival in a changing environment.",
publisher = "Pergamon",
journal = "Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology",
title = "The effect of short-term fasting on the oxidative status of larvae of crested newt species and their hybrids",
volume = "251",
doi = "10.1016/j.cbpa.2020.110819",
pages = "110819"
}

Prokić, M., Petrović, T., Despotović, S., Vučić, T., Gavrić, J., Radovanović, T.,& Gavrilović, B.. (2021). The effect of short-term fasting on the oxidative status of larvae of crested newt species and their hybrids. in Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
Pergamon., 251, 110819.
https://doi.org/10.1016/j.cbpa.2020.110819

Prokić M, Petrović T, Despotović S, Vučić T, Gavrić J, Radovanović T, Gavrilović B. The effect of short-term fasting on the oxidative status of larvae of crested newt species and their hybrids. in Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. 2021;251:110819.
doi:10.1016/j.cbpa.2020.110819 .

Prokić, Marko, Petrović, Tamara, Despotović, Svetlana, Vučić, Tijana, Gavrić, Jelena, Radovanović, Tijana, Gavrilović, Branka, "The effect of short-term fasting on the oxidative status of larvae of crested newt species and their hybrids" in Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 251 (2021):110819,
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