TY  - CONF
AU  - Mijuskovic, Ana
AU  - Tatalovic, Nikola
AU  - Oreščanin Dušić, Zorana
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasić, Mihajlo
AU  - Blagojević, Duško
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1966
C3  - Nitric Oxide-Biology and Chemistry
T1  - Hydrogen sulphide mediated uterine relaxation is not altered by elevated
 homocysteine
VL  - 47
DO  - 10.1016/j.niox.2015.02.080
ER  - 

@conference{
author = "Mijuskovic, Ana and Tatalovic, Nikola and Oreščanin Dušić, Zorana and Nikolić-Kokić, Aleksandra and Spasić, Mihajlo and Blagojević, Duško",
year = "2015",
journal = "Nitric Oxide-Biology and Chemistry",
title = "Hydrogen sulphide mediated uterine relaxation is not altered by elevated
 homocysteine",
volume = "47",
doi = "10.1016/j.niox.2015.02.080"
}

Mijuskovic, A., Tatalovic, N., Oreščanin Dušić, Z., Nikolić-Kokić, A., Spasić, M.,& Blagojević, D.. (2015). Hydrogen sulphide mediated uterine relaxation is not altered by elevated
 homocysteine. in Nitric Oxide-Biology and Chemistry, 47.
https://doi.org/10.1016/j.niox.2015.02.080

Mijuskovic A, Tatalovic N, Oreščanin Dušić Z, Nikolić-Kokić A, Spasić M, Blagojević D. Hydrogen sulphide mediated uterine relaxation is not altered by elevated
 homocysteine. in Nitric Oxide-Biology and Chemistry. 2015;47.
doi:10.1016/j.niox.2015.02.080 .

Mijuskovic, Ana, Tatalovic, Nikola, Oreščanin Dušić, Zorana, Nikolić-Kokić, Aleksandra, Spasić, Mihajlo, Blagojević, Duško, "Hydrogen sulphide mediated uterine relaxation is not altered by elevated
 homocysteine" in Nitric Oxide-Biology and Chemistry, 47 (2015),
https://doi.org/10.1016/j.niox.2015.02.080 . .

TY  - JOUR
AU  - Nikolić-Kokić, Aleksandra
AU  - Oreščanin Dušić, Zorana
AU  - Spasojevic, Ivan
AU  - Slavic, Marija
AU  - Mijuskovic, Ana
AU  - Paskulin, Roman
AU  - Miljevic, Cedo
AU  - Spasić, Mihajlo
AU  - Blagojević, Duško
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1970
AB  - Ethnopharmacological relevance: Ibogaine is a naturally occurring
   alkaloid with psychotropic and metabotropic effects, derived from the
   bark of the root of the West African Tabernanthe iboga plant. The tribes
   of Kongo basin have been using iboga as a stimulant, for medicinal
   purposes, and in rite of passage ceremonies, for centuries. Besides, it
   has been found that this drug has anti-addictive effects.
   Aim of the study: Previous studies have demonstrated that ibogaine
   changed the quantity of ATP and energy related enzymes as well as the
   activity of antioxidant enzymes in cells thus altering redox equilibrium
   in a time manner. In this work, the mechanism of its action was further
   studied by measuring the effects of ibogaine in human erythrocytes in
   vitro on ATP liberation, membrane fluidity and antioxidant enzymes
   activity.
   Materials and methods: Heparinized human blood samples were incubated
   with ibogaine (10 and 20 mu M) at 37 degrees C for 1 h. Blood plasma was
   separated by centrifugation and the levels of ATP and uric acid were
   measured 10 mm after the addition of ibogaine using standard kits. The
   activity of copper zinc superoxide dismutase (SOD1), catalase (CAT),
   glutathione peroxidase (GSH-Px) and glutathione reductase (GR) were
   measured in erythrocytes after incubation period. The stability of SOD1
   activity was further tested through in vitro incubation with H2O2 and
   scanning of its electrophoretic profiles. Membrane fluidity was
   determined using an electron paramagnetic resonance spin-labelling
   method.
   Results: Results showed that ibogaine treatment of erythrocytes in vitro
   increased ATP concentration in the blood plasma without changes in
   neither erythrocytes membrane fluidity nor uric acid concentration.
   lbogaine also increased SOD1 activity in erythrocytes at both doses
   applied here. Treatment with 20 mu M also elevated GR activity after in
   vitro incubation at 37 degrees C. Electrophoretic profiles revealed that
   incubation with ibogaine mitigates H2O2 mediated suppression of SOD1
   activity.
   Conclusion: Some of the effects of ibogaine seem to be mediated through
   its influence on energy metabolism, redox active processes and the
   effects of discrete fluctuations of individual reactive oxygen species
   on different levels of enzyme activities. Overall, ibogaine acts as a
   pro-antioxidant by increasing activity of antioxidative enzymes and as
   an adaptagene in oxidative distress. (C) 2015 Published by Elsevier
   Ireland Ltd.
T2  - Journal of Ethnopharmacology
T1  - Ex vivo effects of ibogaine on the activity of antioxidative enzymes in
 human erythrocytes
VL  - 164
DO  - 10.1016/j.jep.2015.01.037
SP  - 64
EP  - 70
ER  - 

@article{
author = "Nikolić-Kokić, Aleksandra and Oreščanin Dušić, Zorana and Spasojevic, Ivan and Slavic, Marija and Mijuskovic, Ana and Paskulin, Roman and Miljevic, Cedo and Spasić, Mihajlo and Blagojević, Duško",
year = "2015",
abstract = "Ethnopharmacological relevance: Ibogaine is a naturally occurring
   alkaloid with psychotropic and metabotropic effects, derived from the
   bark of the root of the West African Tabernanthe iboga plant. The tribes
   of Kongo basin have been using iboga as a stimulant, for medicinal
   purposes, and in rite of passage ceremonies, for centuries. Besides, it
   has been found that this drug has anti-addictive effects.
   Aim of the study: Previous studies have demonstrated that ibogaine
   changed the quantity of ATP and energy related enzymes as well as the
   activity of antioxidant enzymes in cells thus altering redox equilibrium
   in a time manner. In this work, the mechanism of its action was further
   studied by measuring the effects of ibogaine in human erythrocytes in
   vitro on ATP liberation, membrane fluidity and antioxidant enzymes
   activity.
   Materials and methods: Heparinized human blood samples were incubated
   with ibogaine (10 and 20 mu M) at 37 degrees C for 1 h. Blood plasma was
   separated by centrifugation and the levels of ATP and uric acid were
   measured 10 mm after the addition of ibogaine using standard kits. The
   activity of copper zinc superoxide dismutase (SOD1), catalase (CAT),
   glutathione peroxidase (GSH-Px) and glutathione reductase (GR) were
   measured in erythrocytes after incubation period. The stability of SOD1
   activity was further tested through in vitro incubation with H2O2 and
   scanning of its electrophoretic profiles. Membrane fluidity was
   determined using an electron paramagnetic resonance spin-labelling
   method.
   Results: Results showed that ibogaine treatment of erythrocytes in vitro
   increased ATP concentration in the blood plasma without changes in
   neither erythrocytes membrane fluidity nor uric acid concentration.
   lbogaine also increased SOD1 activity in erythrocytes at both doses
   applied here. Treatment with 20 mu M also elevated GR activity after in
   vitro incubation at 37 degrees C. Electrophoretic profiles revealed that
   incubation with ibogaine mitigates H2O2 mediated suppression of SOD1
   activity.
   Conclusion: Some of the effects of ibogaine seem to be mediated through
   its influence on energy metabolism, redox active processes and the
   effects of discrete fluctuations of individual reactive oxygen species
   on different levels of enzyme activities. Overall, ibogaine acts as a
   pro-antioxidant by increasing activity of antioxidative enzymes and as
   an adaptagene in oxidative distress. (C) 2015 Published by Elsevier
   Ireland Ltd.",
journal = "Journal of Ethnopharmacology",
title = "Ex vivo effects of ibogaine on the activity of antioxidative enzymes in
 human erythrocytes",
volume = "164",
doi = "10.1016/j.jep.2015.01.037",
pages = "64-70"
}

Nikolić-Kokić, A., Oreščanin Dušić, Z., Spasojevic, I., Slavic, M., Mijuskovic, A., Paskulin, R., Miljevic, C., Spasić, M.,& Blagojević, D.. (2015). Ex vivo effects of ibogaine on the activity of antioxidative enzymes in
 human erythrocytes. in Journal of Ethnopharmacology, 164, 64-70.
https://doi.org/10.1016/j.jep.2015.01.037

Nikolić-Kokić A, Oreščanin Dušić Z, Spasojevic I, Slavic M, Mijuskovic A, Paskulin R, Miljevic C, Spasić M, Blagojević D. Ex vivo effects of ibogaine on the activity of antioxidative enzymes in
 human erythrocytes. in Journal of Ethnopharmacology. 2015;164:64-70.
doi:10.1016/j.jep.2015.01.037 .

Nikolić-Kokić, Aleksandra, Oreščanin Dušić, Zorana, Spasojevic, Ivan, Slavic, Marija, Mijuskovic, Ana, Paskulin, Roman, Miljevic, Cedo, Spasić, Mihajlo, Blagojević, Duško, "Ex vivo effects of ibogaine on the activity of antioxidative enzymes in
 human erythrocytes" in Journal of Ethnopharmacology, 164 (2015):64-70,
https://doi.org/10.1016/j.jep.2015.01.037 . .

TY  - JOUR
AU  - Bolic, Bojana
AU  - Mijuskovic, Ana
AU  - Popovic-Bijelic, Ana
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasic, Snezana
AU  - Blagojević, Duško
AU  - Spasić, Mihajlo
AU  - Spasojevic, Ivan
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2329
AB  - Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling
   species, with superoxide dimutases (SOD) are poorly understood. We
   applied low-T EPR spectroscopy to examine the effects of HS-/H2S and
   superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and
   CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD
   and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while
   manganese appears to be released from MnSOD active center. Untreated and
   O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems,
   i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of
   (patho)physiological effects of HS-/H2S. (C) 2015 Elsevier Inc. All
   rights reserved.
T2  - Nitric Oxide-Biology and Chemistry
T1  - Reactions of superoxide dismutases with HS-/H2S and superoxide radical
 anion: An in vitro EPR study
VL  - 51
DO  - 10.1016/j.niox.2015.09.008
SP  - 19
EP  - 23
ER  - 

@article{
author = "Bolic, Bojana and Mijuskovic, Ana and Popovic-Bijelic, Ana and Nikolić-Kokić, Aleksandra and Spasic, Snezana and Blagojević, Duško and Spasić, Mihajlo and Spasojevic, Ivan",
year = "2015",
abstract = "Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling
   species, with superoxide dimutases (SOD) are poorly understood. We
   applied low-T EPR spectroscopy to examine the effects of HS-/H2S and
   superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and
   CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD
   and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while
   manganese appears to be released from MnSOD active center. Untreated and
   O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems,
   i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of
   (patho)physiological effects of HS-/H2S. (C) 2015 Elsevier Inc. All
   rights reserved.",
journal = "Nitric Oxide-Biology and Chemistry",
title = "Reactions of superoxide dismutases with HS-/H2S and superoxide radical
 anion: An in vitro EPR study",
volume = "51",
doi = "10.1016/j.niox.2015.09.008",
pages = "19-23"
}

Bolic, B., Mijuskovic, A., Popovic-Bijelic, A., Nikolić-Kokić, A., Spasic, S., Blagojević, D., Spasić, M.,& Spasojevic, I.. (2015). Reactions of superoxide dismutases with HS-/H2S and superoxide radical
 anion: An in vitro EPR study. in Nitric Oxide-Biology and Chemistry, 51, 19-23.
https://doi.org/10.1016/j.niox.2015.09.008

Bolic B, Mijuskovic A, Popovic-Bijelic A, Nikolić-Kokić A, Spasic S, Blagojević D, Spasić M, Spasojevic I. Reactions of superoxide dismutases with HS-/H2S and superoxide radical
 anion: An in vitro EPR study. in Nitric Oxide-Biology and Chemistry. 2015;51:19-23.
doi:10.1016/j.niox.2015.09.008 .

Bolic, Bojana, Mijuskovic, Ana, Popovic-Bijelic, Ana, Nikolić-Kokić, Aleksandra, Spasic, Snezana, Blagojević, Duško, Spasić, Mihajlo, Spasojevic, Ivan, "Reactions of superoxide dismutases with HS-/H2S and superoxide radical
 anion: An in vitro EPR study" in Nitric Oxide-Biology and Chemistry, 51 (2015):19-23,
https://doi.org/10.1016/j.niox.2015.09.008 . .

TY  - JOUR
AU  - Berenyiova, Andrea
AU  - Grman, Marian
AU  - Mijuskovic, Ana
AU  - Stasko, Andrej
AU  - Misak, Anton
AU  - Nagy, Peter
AU  - Ondriasova, Elena
AU  - Cacanyiova, Sona
AU  - Brezova, Vlasta
AU  - Feelisch, Martin
AU  - Ondrias, Karol
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1969
AB  - The chemical interaction of sodium sulfide (Na2S) with the NO-donor
   S-nitrosoglutathione (GSNO) has been described to generate new reaction
   products, including polysulfides and nitrosopersulfide (SSNO-) via
   intermediacy of thionitrous acid (HSNO). The aim of the present work was
   to investigate the vascular effects of the longer-lived products of the
   Sulfide/GSNO interaction. Here we show that the products of this
   reaction relax precontracted isolated rings of rat thoracic aorta and
   mesenteric artery (but to a lesser degree rat uterus) with a >2-fold
   potency compared with the starting material, GSNO (50 nM), whereas Na2S
   and polysulfides have little effect at 1-5 mu M. The onset of
   vasorelaxation of the reaction products was 7-10 times faster in aorta
   and mesenteric arteries compared with GSNO. Relaxation to GSNO (100-500
   nM) was blocked by an inhibitor of soluble guanylyl cyclase, ODQ (0.1
   and 10 mu M), and by the NO scavenger cPTIO (100 mu M), but less
   affected by prior acidification (pH 2-4), and unaffected by
   N-acetylcysteine (1 mM) or methemoglobin (20 mu M heme). By contrast,
   relaxation to the Sulfide/GSNO reaction products (100-500 nM based on
   the starting material) was inhibited to a lesser extent by ODQ only
   slightly decreased by cPTIO, more markedly inhibited by methemoglobin
   and N-acetylcysteine, and abolished by acidification before addition to
   the organ bath. The reaction mixture was found to generate NO as
   detected by EPR spectroscopy using
   N-(dithiocarboxy)-N-methyl-D-glucamine (MGD(2))-Fe2+ as spin trap. In
   conclusion, the Sufide/GSNO reaction products are faster and more
   pronounced vasorelaxants than GSNO itself. We conclude that in addition
   to NO formation from SSNO-, reaction products other than polysulfides
   may give rise to nitroxyl (HNO) and be involved in the pronounced
   relaxation induced by the Sulfide/GSNO cross-talk. (C) 2014 Elsevier
   Inc. All rights reserved.
T2  - Nitric Oxide-Biology and Chemistry
T1  - The reaction products of sulfide and S-nitrosoglutathione are potent
 vasorelaxants
IS  - SI
VL  - 46
DO  - 10.1016/j.niox.2014.12.008
SP  - 123
EP  - 130
ER  - 

@article{
author = "Berenyiova, Andrea and Grman, Marian and Mijuskovic, Ana and Stasko, Andrej and Misak, Anton and Nagy, Peter and Ondriasova, Elena and Cacanyiova, Sona and Brezova, Vlasta and Feelisch, Martin and Ondrias, Karol",
year = "2015",
abstract = "The chemical interaction of sodium sulfide (Na2S) with the NO-donor
   S-nitrosoglutathione (GSNO) has been described to generate new reaction
   products, including polysulfides and nitrosopersulfide (SSNO-) via
   intermediacy of thionitrous acid (HSNO). The aim of the present work was
   to investigate the vascular effects of the longer-lived products of the
   Sulfide/GSNO interaction. Here we show that the products of this
   reaction relax precontracted isolated rings of rat thoracic aorta and
   mesenteric artery (but to a lesser degree rat uterus) with a >2-fold
   potency compared with the starting material, GSNO (50 nM), whereas Na2S
   and polysulfides have little effect at 1-5 mu M. The onset of
   vasorelaxation of the reaction products was 7-10 times faster in aorta
   and mesenteric arteries compared with GSNO. Relaxation to GSNO (100-500
   nM) was blocked by an inhibitor of soluble guanylyl cyclase, ODQ (0.1
   and 10 mu M), and by the NO scavenger cPTIO (100 mu M), but less
   affected by prior acidification (pH 2-4), and unaffected by
   N-acetylcysteine (1 mM) or methemoglobin (20 mu M heme). By contrast,
   relaxation to the Sulfide/GSNO reaction products (100-500 nM based on
   the starting material) was inhibited to a lesser extent by ODQ only
   slightly decreased by cPTIO, more markedly inhibited by methemoglobin
   and N-acetylcysteine, and abolished by acidification before addition to
   the organ bath. The reaction mixture was found to generate NO as
   detected by EPR spectroscopy using
   N-(dithiocarboxy)-N-methyl-D-glucamine (MGD(2))-Fe2+ as spin trap. In
   conclusion, the Sufide/GSNO reaction products are faster and more
   pronounced vasorelaxants than GSNO itself. We conclude that in addition
   to NO formation from SSNO-, reaction products other than polysulfides
   may give rise to nitroxyl (HNO) and be involved in the pronounced
   relaxation induced by the Sulfide/GSNO cross-talk. (C) 2014 Elsevier
   Inc. All rights reserved.",
journal = "Nitric Oxide-Biology and Chemistry",
title = "The reaction products of sulfide and S-nitrosoglutathione are potent
 vasorelaxants",
number = "SI",
volume = "46",
doi = "10.1016/j.niox.2014.12.008",
pages = "123-130"
}

Berenyiova, A., Grman, M., Mijuskovic, A., Stasko, A., Misak, A., Nagy, P., Ondriasova, E., Cacanyiova, S., Brezova, V., Feelisch, M.,& Ondrias, K.. (2015). The reaction products of sulfide and S-nitrosoglutathione are potent
 vasorelaxants. in Nitric Oxide-Biology and Chemistry, 46(SI), 123-130.
https://doi.org/10.1016/j.niox.2014.12.008

Berenyiova A, Grman M, Mijuskovic A, Stasko A, Misak A, Nagy P, Ondriasova E, Cacanyiova S, Brezova V, Feelisch M, Ondrias K. The reaction products of sulfide and S-nitrosoglutathione are potent
 vasorelaxants. in Nitric Oxide-Biology and Chemistry. 2015;46(SI):123-130.
doi:10.1016/j.niox.2014.12.008 .

Berenyiova, Andrea, Grman, Marian, Mijuskovic, Ana, Stasko, Andrej, Misak, Anton, Nagy, Peter, Ondriasova, Elena, Cacanyiova, Sona, Brezova, Vlasta, Feelisch, Martin, Ondrias, Karol, "The reaction products of sulfide and S-nitrosoglutathione are potent
 vasorelaxants" in Nitric Oxide-Biology and Chemistry, 46, no. SI (2015):123-130,
https://doi.org/10.1016/j.niox.2014.12.008 . .

TY  - CONF
AU  - Mijuskovic, Ana
AU  - Oreščanin Dušić, Zorana
AU  - Nikolić-Kokić, Aleksandra
AU  - Slavic, Marija
AU  - Spasić, Mihajlo
AU  - Spasojevic, Ivan
AU  - Blagojević, Duško
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2211
C3  - Nitric Oxide-Biology and Chemistry
T1  - Sodium sulphide relaxation of rat uterus is related to calcium signaling
IS  - 1
VL  - 39
DO  - 10.1016/j.niox.2014.03.119
SP  - S36
EP  - S37
ER  - 

@conference{
author = "Mijuskovic, Ana and Oreščanin Dušić, Zorana and Nikolić-Kokić, Aleksandra and Slavic, Marija and Spasić, Mihajlo and Spasojevic, Ivan and Blagojević, Duško",
year = "2014",
journal = "Nitric Oxide-Biology and Chemistry",
title = "Sodium sulphide relaxation of rat uterus is related to calcium signaling",
number = "1",
volume = "39",
doi = "10.1016/j.niox.2014.03.119",
pages = "S36-S37"
}

Mijuskovic, A., Oreščanin Dušić, Z., Nikolić-Kokić, A., Slavic, M., Spasić, M., Spasojevic, I.,& Blagojević, D.. (2014). Sodium sulphide relaxation of rat uterus is related to calcium signaling. in Nitric Oxide-Biology and Chemistry, 39(1), S36-S37.
https://doi.org/10.1016/j.niox.2014.03.119

Mijuskovic A, Oreščanin Dušić Z, Nikolić-Kokić A, Slavic M, Spasić M, Spasojevic I, Blagojević D. Sodium sulphide relaxation of rat uterus is related to calcium signaling. in Nitric Oxide-Biology and Chemistry. 2014;39(1):S36-S37.
doi:10.1016/j.niox.2014.03.119 .

Mijuskovic, Ana, Oreščanin Dušić, Zorana, Nikolić-Kokić, Aleksandra, Slavic, Marija, Spasić, Mihajlo, Spasojevic, Ivan, Blagojević, Duško, "Sodium sulphide relaxation of rat uterus is related to calcium signaling" in Nitric Oxide-Biology and Chemistry, 39, no. 1 (2014):S36-S37,
https://doi.org/10.1016/j.niox.2014.03.119 . .

TY  - JOUR
AU  - Brkljačić, Jelena
AU  - Glban, Alhadi M.
AU  - Mijuskovic, Ana
AU  - Nikolić-Kokić, Aleksandra
AU  - Elaković, Ivana
AU  - Velickovic, Natasa
AU  - Matić, Gordana
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2192
AB  - Increased fructose consumption is correlated with the rising prevalence
   of obesity, metabolic syndrome, and type 2 diabetes. It is believed that
   reactive oxygen species contribute to the development and progression of
   metabolic disturbances, especially those associated with insulin
   resistance. Dietary fructose produces both pro-oxidative and
   antioxidative effects, depending upon the experimental conditions,
   dosage, duration of treatment, and pathophysiological milieu. The
   effects of fructose overconsumption on young populations, which have an
   increased risk of developing metabolic disorders in adulthood, have not
   been fully elucidated. We have previously shown that rats subjected to a
   long-term fructose-enriched diet immediately after weaning display
   impaired hepatic insulin sensitivity. In this study, we tested the
   hypothesis that long-term fructose consumption induces alterations in
   the redox setting of the liver. Starting from the 21st day afterbirth,
   male Wistar rats were maintained for 9 weeks on a standard diet
   (control) or a fructose-enriched diet that consisted of standard food
   and 10\% fructose solution instead of drinking water. The expression and
   activity of antioxidant enzymes as well as lipid peroxidation and
   protein damage markers were measured. The results showed that a
   fructose-enriched diet led to an increased expression of mitochondrial
   manganese superoxide dismutase but did not affect antioxidant enzymes
   activity, lipid peroxidation, thiol content, and the level of protein
   oxidation. Therefore, our results suggest that the decrease in hepatic
   insulin sensitivity that was previously observed in rats that were kept
   on the same diet regime might be attributed to molecular mechanisms
   other than redox disbalance. A possible fructose-related micronutrient
   deficiency should be examined. (C) 2014 Elsevier Inc. All rights
   reserved.
T2  - Nutrition Research
T1  - Long-term fructose-enriched diet introduced immediately after weaning
 does not induce oxidative stress in the rat liver
IS  - 7
VL  - 34
DO  - 10.1016/j.nutres.2014.06.006
SP  - 646
EP  - 652
ER  - 

@article{
author = "Brkljačić, Jelena and Glban, Alhadi M. and Mijuskovic, Ana and Nikolić-Kokić, Aleksandra and Elaković, Ivana and Velickovic, Natasa and Matić, Gordana",
year = "2014",
abstract = "Increased fructose consumption is correlated with the rising prevalence
   of obesity, metabolic syndrome, and type 2 diabetes. It is believed that
   reactive oxygen species contribute to the development and progression of
   metabolic disturbances, especially those associated with insulin
   resistance. Dietary fructose produces both pro-oxidative and
   antioxidative effects, depending upon the experimental conditions,
   dosage, duration of treatment, and pathophysiological milieu. The
   effects of fructose overconsumption on young populations, which have an
   increased risk of developing metabolic disorders in adulthood, have not
   been fully elucidated. We have previously shown that rats subjected to a
   long-term fructose-enriched diet immediately after weaning display
   impaired hepatic insulin sensitivity. In this study, we tested the
   hypothesis that long-term fructose consumption induces alterations in
   the redox setting of the liver. Starting from the 21st day afterbirth,
   male Wistar rats were maintained for 9 weeks on a standard diet
   (control) or a fructose-enriched diet that consisted of standard food
   and 10\% fructose solution instead of drinking water. The expression and
   activity of antioxidant enzymes as well as lipid peroxidation and
   protein damage markers were measured. The results showed that a
   fructose-enriched diet led to an increased expression of mitochondrial
   manganese superoxide dismutase but did not affect antioxidant enzymes
   activity, lipid peroxidation, thiol content, and the level of protein
   oxidation. Therefore, our results suggest that the decrease in hepatic
   insulin sensitivity that was previously observed in rats that were kept
   on the same diet regime might be attributed to molecular mechanisms
   other than redox disbalance. A possible fructose-related micronutrient
   deficiency should be examined. (C) 2014 Elsevier Inc. All rights
   reserved.",
journal = "Nutrition Research",
title = "Long-term fructose-enriched diet introduced immediately after weaning
 does not induce oxidative stress in the rat liver",
number = "7",
volume = "34",
doi = "10.1016/j.nutres.2014.06.006",
pages = "646-652"
}

Brkljačić, J., Glban, A. M., Mijuskovic, A., Nikolić-Kokić, A., Elaković, I., Velickovic, N.,& Matić, G.. (2014). Long-term fructose-enriched diet introduced immediately after weaning
 does not induce oxidative stress in the rat liver. in Nutrition Research, 34(7), 646-652.
https://doi.org/10.1016/j.nutres.2014.06.006

Brkljačić J, Glban AM, Mijuskovic A, Nikolić-Kokić A, Elaković I, Velickovic N, Matić G. Long-term fructose-enriched diet introduced immediately after weaning
 does not induce oxidative stress in the rat liver. in Nutrition Research. 2014;34(7):646-652.
doi:10.1016/j.nutres.2014.06.006 .

Brkljačić, Jelena, Glban, Alhadi M., Mijuskovic, Ana, Nikolić-Kokić, Aleksandra, Elaković, Ivana, Velickovic, Natasa, Matić, Gordana, "Long-term fructose-enriched diet introduced immediately after weaning
 does not induce oxidative stress in the rat liver" in Nutrition Research, 34, no. 7 (2014):646-652,
https://doi.org/10.1016/j.nutres.2014.06.006 . .

TY  - JOUR
AU  - Mijuskovic, Ana
AU  - Oreščanin Dušić, Zorana
AU  - Nikolić-Kokić, Aleksandra
AU  - Slavic, Marija
AU  - Spasić, Mihajlo
AU  - Spasojevic, Ivan
AU  - Blagojević, Duško
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2206
AB  - Background: Our aim was to investigate the effect of methanethiol
   (CH3SH) on contractility of rat uterus and activities of redox-active
   enzymes, and to compare them with the effect of sodium sulphide (Na2S),
   a hydrogen sulphide (H2S/HS-) donor.
   Methods: Uteri were isolated from virgin Wistar rats, divided into six
   groups, controls (untreated uteri allowed to contract spontaneously and
   in the presence of Ca2+(6 mM)), CH3SH treated (spontaneously active and
   Ca2+ induced) and Na2S treated (spontaneously active and Ca2+ induced).
   Underlying antioxidative enzyme activities (superoxide dismutase - SOD,
   glutathione peroxidase - GSHPx, glutathione reductase - GR) in CH3SH- or
   Na2S-treated uteri were compared to controls.
   Results: Our experiments showed that CH3SH and Na2S provoked reversible
   relaxation of both spontaneous and Ca2+ induced uterine contractions.
   The dose-response curves differed in shape, and CH3SH curve was shifted
   to higher concentration compared to H2S/HS-. The effects of Na2S fitted
   sigmoid curve, whereas those of CH3SH fitted linearly. CH3SH provoked
   increased SOD activity and decreased GR activity. However, Na2S
   (H2S/HS-) provoked an increase in SOD activity exclusively in Ca2+
   stimulated uteri, while the activity of GSHPx was increased in both
   types of active uteri.
   Conclusion: Our results imply that CH3SH may have a constructive role in
   the control of muscle function and metabolism. Observed differences
   between CH3SH and H2S/HS- could be attributed to a larger moiety that is
   present in CH3SH compared to H2S, but they are more likely to be a
   consequence of the specific actions of HS-, in relation to its negative
   charge. (C) 2014 Institute of Pharmacology, Polish Academy of Sciences.
   Published by Elsevier Urban \& Partner Sp. Z o.o.. All rights reserved.
T2  - Pharmacological Reports
T1  - Comparison of the effects of methanethiol and sodium sulphide on uterine
 contractile activity
IS  - 3
VL  - 66
DO  - 10.1016/j.pharep.2013.12.012
SP  - 373
EP  - 379
ER  - 

@article{
author = "Mijuskovic, Ana and Oreščanin Dušić, Zorana and Nikolić-Kokić, Aleksandra and Slavic, Marija and Spasić, Mihajlo and Spasojevic, Ivan and Blagojević, Duško",
year = "2014",
abstract = "Background: Our aim was to investigate the effect of methanethiol
   (CH3SH) on contractility of rat uterus and activities of redox-active
   enzymes, and to compare them with the effect of sodium sulphide (Na2S),
   a hydrogen sulphide (H2S/HS-) donor.
   Methods: Uteri were isolated from virgin Wistar rats, divided into six
   groups, controls (untreated uteri allowed to contract spontaneously and
   in the presence of Ca2+(6 mM)), CH3SH treated (spontaneously active and
   Ca2+ induced) and Na2S treated (spontaneously active and Ca2+ induced).
   Underlying antioxidative enzyme activities (superoxide dismutase - SOD,
   glutathione peroxidase - GSHPx, glutathione reductase - GR) in CH3SH- or
   Na2S-treated uteri were compared to controls.
   Results: Our experiments showed that CH3SH and Na2S provoked reversible
   relaxation of both spontaneous and Ca2+ induced uterine contractions.
   The dose-response curves differed in shape, and CH3SH curve was shifted
   to higher concentration compared to H2S/HS-. The effects of Na2S fitted
   sigmoid curve, whereas those of CH3SH fitted linearly. CH3SH provoked
   increased SOD activity and decreased GR activity. However, Na2S
   (H2S/HS-) provoked an increase in SOD activity exclusively in Ca2+
   stimulated uteri, while the activity of GSHPx was increased in both
   types of active uteri.
   Conclusion: Our results imply that CH3SH may have a constructive role in
   the control of muscle function and metabolism. Observed differences
   between CH3SH and H2S/HS- could be attributed to a larger moiety that is
   present in CH3SH compared to H2S, but they are more likely to be a
   consequence of the specific actions of HS-, in relation to its negative
   charge. (C) 2014 Institute of Pharmacology, Polish Academy of Sciences.
   Published by Elsevier Urban \& Partner Sp. Z o.o.. All rights reserved.",
journal = "Pharmacological Reports",
title = "Comparison of the effects of methanethiol and sodium sulphide on uterine
 contractile activity",
number = "3",
volume = "66",
doi = "10.1016/j.pharep.2013.12.012",
pages = "373-379"
}

Mijuskovic, A., Oreščanin Dušić, Z., Nikolić-Kokić, A., Slavic, M., Spasić, M., Spasojevic, I.,& Blagojević, D.. (2014). Comparison of the effects of methanethiol and sodium sulphide on uterine
 contractile activity. in Pharmacological Reports, 66(3), 373-379.
https://doi.org/10.1016/j.pharep.2013.12.012

Mijuskovic A, Oreščanin Dušić Z, Nikolić-Kokić A, Slavic M, Spasić M, Spasojevic I, Blagojević D. Comparison of the effects of methanethiol and sodium sulphide on uterine
 contractile activity. in Pharmacological Reports. 2014;66(3):373-379.
doi:10.1016/j.pharep.2013.12.012 .

Mijuskovic, Ana, Oreščanin Dušić, Zorana, Nikolić-Kokić, Aleksandra, Slavic, Marija, Spasić, Mihajlo, Spasojevic, Ivan, Blagojević, Duško, "Comparison of the effects of methanethiol and sodium sulphide on uterine
 contractile activity" in Pharmacological Reports, 66, no. 3 (2014):373-379,
https://doi.org/10.1016/j.pharep.2013.12.012 . .