TY  - JOUR
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka
AU  - Ajdžanović, Vladimir
AU  - Brkić, Dejan
AU  - Manojlović Stojanoski, Milica
AU  - Milošević, Verica
AU  - Sekulić, Milka
PY  - 2010
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6131
AB  - Thyroid C cells hormone, calcitonine, inhibits bone resorption. We have demonstrated that daidzein treatment of orchidectomized rats (model for osteoporosis) stimulated C cells and increased trabecular bone mass. These results suggest that, besides direct action, daidzein may also affect bone structure indirectly through enhancement of thyroid C cell activity. Thyroid C cells produce calcitonin (CT) which acts as an inhibitor of bone resorption. In this study, the influence of daidzein treatment on thyroid C cells, bone structure, and bone function in orchidectomized (Orx) middle-aged rats was investigated. Sixteen-month-old Wistar rats were divided into Orx and sham-operated (SO) groups. Half the Orx rats were given subcutaneous injections of daidzein (30 mg/kg b.w./day) for 3 weeks. CT-immunopositive thyroid C cells were morphometrically analyzed. The metaphyseal region of the proximal tibia was measured histomorphometrically, and cancellous bone area (B.Ar), trabecular thickness (Tb.Th), trabecular number (Tb.N), and trabecular separation (Tb.Sp) were calculated. Serum samples were analyzed for CT and osteocalcin (OC), calcium (Ca) and phosphorus concentrations, and urine samples for Ca levels. Treatment of Orx animals with daidzein significantly increased volume of C cells compared to the Orx rats. Daidzein also enhanced B.Ar, Tb.Th, and Tb.N and reduced Tb.Sp. The serum OC and urinary Ca concentrations decreased significantly in comparison with the Orx group. These findings indicate that daidzein treatment stimulates thyroid C cells, increase trabecular bone mass, and decrease bone turnover in Orx middle-aged rats, which is the model of male osteoporosis.
PB  - Springer Nature
T2  - Osteoporosis International
T1  - Daidzein administration positively affects thyroid C cells and bone structure in orchidectomized middle-aged rats
IS  - 9
VL  - 21
DO  - 10.1007/s00198-009-1092-x
SP  - 1609
EP  - 1616
ER  - 

@article{
author = "Filipović, Branko and Šošić-Jurjević, Branka and Ajdžanović, Vladimir and Brkić, Dejan and Manojlović Stojanoski, Milica and Milošević, Verica and Sekulić, Milka",
year = "2010",
abstract = "Thyroid C cells hormone, calcitonine, inhibits bone resorption. We have demonstrated that daidzein treatment of orchidectomized rats (model for osteoporosis) stimulated C cells and increased trabecular bone mass. These results suggest that, besides direct action, daidzein may also affect bone structure indirectly through enhancement of thyroid C cell activity. Thyroid C cells produce calcitonin (CT) which acts as an inhibitor of bone resorption. In this study, the influence of daidzein treatment on thyroid C cells, bone structure, and bone function in orchidectomized (Orx) middle-aged rats was investigated. Sixteen-month-old Wistar rats were divided into Orx and sham-operated (SO) groups. Half the Orx rats were given subcutaneous injections of daidzein (30 mg/kg b.w./day) for 3 weeks. CT-immunopositive thyroid C cells were morphometrically analyzed. The metaphyseal region of the proximal tibia was measured histomorphometrically, and cancellous bone area (B.Ar), trabecular thickness (Tb.Th), trabecular number (Tb.N), and trabecular separation (Tb.Sp) were calculated. Serum samples were analyzed for CT and osteocalcin (OC), calcium (Ca) and phosphorus concentrations, and urine samples for Ca levels. Treatment of Orx animals with daidzein significantly increased volume of C cells compared to the Orx rats. Daidzein also enhanced B.Ar, Tb.Th, and Tb.N and reduced Tb.Sp. The serum OC and urinary Ca concentrations decreased significantly in comparison with the Orx group. These findings indicate that daidzein treatment stimulates thyroid C cells, increase trabecular bone mass, and decrease bone turnover in Orx middle-aged rats, which is the model of male osteoporosis.",
publisher = "Springer Nature",
journal = "Osteoporosis International",
title = "Daidzein administration positively affects thyroid C cells and bone structure in orchidectomized middle-aged rats",
number = "9",
volume = "21",
doi = "10.1007/s00198-009-1092-x",
pages = "1609-1616"
}

Filipović, B., Šošić-Jurjević, B., Ajdžanović, V., Brkić, D., Manojlović Stojanoski, M., Milošević, V.,& Sekulić, M.. (2010). Daidzein administration positively affects thyroid C cells and bone structure in orchidectomized middle-aged rats. in Osteoporosis International
Springer Nature., 21(9), 1609-1616.
https://doi.org/10.1007/s00198-009-1092-x

Filipović B, Šošić-Jurjević B, Ajdžanović V, Brkić D, Manojlović Stojanoski M, Milošević V, Sekulić M. Daidzein administration positively affects thyroid C cells and bone structure in orchidectomized middle-aged rats. in Osteoporosis International. 2010;21(9):1609-1616.
doi:10.1007/s00198-009-1092-x .

Filipović, Branko, Šošić-Jurjević, Branka, Ajdžanović, Vladimir, Brkić, Dejan, Manojlović Stojanoski, Milica, Milošević, Verica, Sekulić, Milka, "Daidzein administration positively affects thyroid C cells and bone structure in orchidectomized middle-aged rats" in Osteoporosis International, 21, no. 9 (2010):1609-1616,
https://doi.org/10.1007/s00198-009-1092-x . .

TY  - JOUR
AU  - Šošić-Jurjević, Branka 
AU  - Filipović, Branko
AU  - Ajdžanović, Vladimir
AU  - Brkić, Dejan
AU  - Ristić, Nataša
AU  - Manojlović-Stojanoski, Milica
AU  - Nestorović, Nataša
AU  - Trifunović, Svetlana
AU  - Sekulić, Milka
PY  - 2007
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6099
AB  - Nutritional supplements containing soybean phytoestrogens, the isoflavones genistein (G) and daidzein (D), are increasingly used as alternative therapy for osteoporosis, cancer, and cardiovascular and other diseases with a frequency that increases with advancing age. In this study we examined the effects of subcutaneous administration of either G or D on serum lipid levels in orchidectomized (Orx) and intact (IA) middle-aged male rats, which are experimental models of andropause. Sixteen-month-old Wistar rats were treated with 10 mg/kg and 30 mg/kg of either G or D. The control groups received testosterone, estradiol, or vehicle for 3 weeks, after which the total serum cholesterol (TC), low-density lipoprotein (LDL-C), high-density lipoprotein (HDL-C), and total triglycerides (TT) were measured. Compared with the matching vehicle-treated controls, the higher doses of G and D and testosterone treatment significantly (P < 0.05) lowered the TC and lipoprotein cholesterol levels. The greatest effect was observed regarding LDL-C in both Orx and IA males after G and D treatments, in which LDL-C decreased by more than 30%. The lower isoflavone doses induced a significant cholesterol-lowering effect (P < 0.05) only in the Orx group. Like the estradiol treatment, the higher doses of G and D increased the TT levels in both rat models by more than 50% (P < 0.05). The lower doses of isoflavones increased TT only in the Orx group. In male middle-aged rats, injections of higher doses of G and D decreased the serum cholesterol levels, as did testosterone injection, and brought about an increase in serum triglycerides similar to that observed after estradiol treatment.
PB  - SAGE Publications
T2  - Experimental biology and medicine (Maywood, N.J.)
T1  - Subcutaneously administrated genistein and daidzein decrease serum cholesterol and increase triglyceride levels in male middle-aged rats
IS  - 9
VL  - 232
DO  - 10.3181/0703-BC-82
SP  - 1222
EP  - 1227
ER  - 

@article{
author = "Šošić-Jurjević, Branka  and Filipović, Branko and Ajdžanović, Vladimir and Brkić, Dejan and Ristić, Nataša and Manojlović-Stojanoski, Milica and Nestorović, Nataša and Trifunović, Svetlana and Sekulić, Milka",
year = "2007",
abstract = "Nutritional supplements containing soybean phytoestrogens, the isoflavones genistein (G) and daidzein (D), are increasingly used as alternative therapy for osteoporosis, cancer, and cardiovascular and other diseases with a frequency that increases with advancing age. In this study we examined the effects of subcutaneous administration of either G or D on serum lipid levels in orchidectomized (Orx) and intact (IA) middle-aged male rats, which are experimental models of andropause. Sixteen-month-old Wistar rats were treated with 10 mg/kg and 30 mg/kg of either G or D. The control groups received testosterone, estradiol, or vehicle for 3 weeks, after which the total serum cholesterol (TC), low-density lipoprotein (LDL-C), high-density lipoprotein (HDL-C), and total triglycerides (TT) were measured. Compared with the matching vehicle-treated controls, the higher doses of G and D and testosterone treatment significantly (P < 0.05) lowered the TC and lipoprotein cholesterol levels. The greatest effect was observed regarding LDL-C in both Orx and IA males after G and D treatments, in which LDL-C decreased by more than 30%. The lower isoflavone doses induced a significant cholesterol-lowering effect (P < 0.05) only in the Orx group. Like the estradiol treatment, the higher doses of G and D increased the TT levels in both rat models by more than 50% (P < 0.05). The lower doses of isoflavones increased TT only in the Orx group. In male middle-aged rats, injections of higher doses of G and D decreased the serum cholesterol levels, as did testosterone injection, and brought about an increase in serum triglycerides similar to that observed after estradiol treatment.",
publisher = "SAGE Publications",
journal = "Experimental biology and medicine (Maywood, N.J.)",
title = "Subcutaneously administrated genistein and daidzein decrease serum cholesterol and increase triglyceride levels in male middle-aged rats",
number = "9",
volume = "232",
doi = "10.3181/0703-BC-82",
pages = "1222-1227"
}

Šošić-Jurjević, B., Filipović, B., Ajdžanović, V., Brkić, D., Ristić, N., Manojlović-Stojanoski, M., Nestorović, N., Trifunović, S.,& Sekulić, M.. (2007). Subcutaneously administrated genistein and daidzein decrease serum cholesterol and increase triglyceride levels in male middle-aged rats. in Experimental biology and medicine (Maywood, N.J.)
SAGE Publications., 232(9), 1222-1227.
https://doi.org/10.3181/0703-BC-82

Šošić-Jurjević B, Filipović B, Ajdžanović V, Brkić D, Ristić N, Manojlović-Stojanoski M, Nestorović N, Trifunović S, Sekulić M. Subcutaneously administrated genistein and daidzein decrease serum cholesterol and increase triglyceride levels in male middle-aged rats. in Experimental biology and medicine (Maywood, N.J.). 2007;232(9):1222-1227.
doi:10.3181/0703-BC-82 .

Šošić-Jurjević, Branka , Filipović, Branko, Ajdžanović, Vladimir, Brkić, Dejan, Ristić, Nataša, Manojlović-Stojanoski, Milica, Nestorović, Nataša, Trifunović, Svetlana, Sekulić, Milka, "Subcutaneously administrated genistein and daidzein decrease serum cholesterol and increase triglyceride levels in male middle-aged rats" in Experimental biology and medicine (Maywood, N.J.), 232, no. 9 (2007):1222-1227,
https://doi.org/10.3181/0703-BC-82 . .

TY  - JOUR
AU  - Rančić, Ana
AU  - Soković, Marina
AU  - van Griensven, Leo JLD
AU  - Vukojević, Jelena B.
AU  - Brkić, Dejan
AU  - Ristić, Mihailo S.
PY  - 2003
PY  - 2003
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/406
AB  - In this article we investigated antibacterial and antifungal activities of limonene against 7 bacterial and 19 fungal species. Antibacterial activity and Candida albicans were investigated by on TLC plates, while antifungal activity was tested by micro- and macrodilution tests. Limonene showed antimicrobial activities against all microorganisms tested. The best results were obtained against Micrococcus luteus, Streptococcus epidermidis, S. aureus and Salmonella typhimurium, in concentration of 5 ml. Limonene exhibited antifungal activity in concentration of 8.0 - 13.0 ml/ml in macrodilution and 6.0-10.0 ml/ml in microdilution method.
AB  - U ovom radu je ispitivano antibakterijsko i antifungalno dejstvo limonena na 7 bakterijskih i 19 gljivičnih vrsta. Antibakterijska aktivnost i Candida albicans su testirani bioautografskim testom na TLC pločama, dok je antifungalna mikro- i makrodilucionom metodom. Limonen je pokazao antimikrobnu aktivnost na sve testirane organizme. Najbolje dejstvo je imao na Micrococcus luteus, Streptococcus epidermidis, S. aureus i Salmonella typhimurium, u koncentraciji od 5 ml. Takođe, limonen je delovao i antifungalno u koncentraciji od 8.0-13.0 ml/ml u macrodilucionoj i 6.0-10.0 ml/ml u microdilucionoj metodi.
T2  - Lekovite sirovine
T1  - Antimikrobno dejstvo limonena
T1  - Antimicrobial activity of limonene
IS  - 23
VL  - null
SP  - 83
EP  - 88
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_406
ER  - 

@article{
author = "Rančić, Ana and Soković, Marina and van Griensven, Leo JLD and Vukojević, Jelena B. and Brkić, Dejan and Ristić, Mihailo S.",
year = "2003, 2003",
abstract = "In this article we investigated antibacterial and antifungal activities of limonene against 7 bacterial and 19 fungal species. Antibacterial activity and Candida albicans were investigated by on TLC plates, while antifungal activity was tested by micro- and macrodilution tests. Limonene showed antimicrobial activities against all microorganisms tested. The best results were obtained against Micrococcus luteus, Streptococcus epidermidis, S. aureus and Salmonella typhimurium, in concentration of 5 ml. Limonene exhibited antifungal activity in concentration of 8.0 - 13.0 ml/ml in macrodilution and 6.0-10.0 ml/ml in microdilution method., U ovom radu je ispitivano antibakterijsko i antifungalno dejstvo limonena na 7 bakterijskih i 19 gljivičnih vrsta. Antibakterijska aktivnost i Candida albicans su testirani bioautografskim testom na TLC pločama, dok je antifungalna mikro- i makrodilucionom metodom. Limonen je pokazao antimikrobnu aktivnost na sve testirane organizme. Najbolje dejstvo je imao na Micrococcus luteus, Streptococcus epidermidis, S. aureus i Salmonella typhimurium, u koncentraciji od 5 ml. Takođe, limonen je delovao i antifungalno u koncentraciji od 8.0-13.0 ml/ml u macrodilucionoj i 6.0-10.0 ml/ml u microdilucionoj metodi.",
journal = "Lekovite sirovine",
title = "Antimikrobno dejstvo limonena, Antimicrobial activity of limonene",
number = "23",
volume = "null",
pages = "83-88",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_406"
}

Rančić, A., Soković, M., van Griensven, L. J., Vukojević, J. B., Brkić, D.,& Ristić, M. S.. (2003). Antimikrobno dejstvo limonena. in Lekovite sirovine, null(23), 83-88.
https://hdl.handle.net/21.15107/rcub_ibiss_406

Rančić A, Soković M, van Griensven LJ, Vukojević JB, Brkić D, Ristić MS. Antimikrobno dejstvo limonena. in Lekovite sirovine. 2003;null(23):83-88.
https://hdl.handle.net/21.15107/rcub_ibiss_406 .

Rančić, Ana, Soković, Marina, van Griensven, Leo JLD, Vukojević, Jelena B., Brkić, Dejan, Ristić, Mihailo S., "Antimikrobno dejstvo limonena" in Lekovite sirovine, null, no. 23 (2003):83-88,
https://hdl.handle.net/21.15107/rcub_ibiss_406 .