TY  - JOUR
AU  - Šošić-Jurjević, Branka
AU  - Borković-Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Vlahović, Dragana
AU  - Miler, Marko
AU  - Cesar, Thais
AU  - Ajdžanović, Vladimir
AU  - Milenković, Dragan
AU  - Stellaard, Frans
AU  - Trifunović, Svetlana
AU  - Filipović, Branko
AU  - Dieter, Lutjohann
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6702
AB  - This study aimed to assess the antioxidant capacity of lemon flavonoid extract Eriomin®
(LE) and its impact on cholesterol metabolism in the context of healthy aging. We orally treated 24-
month-old male Wistar rats with an LE (40 mg/kg) suspended in 0.3 mL of sunflower oil. At the
same time, control groups received an equal volume of sunflower oil (CON) or remained untreated
(ICON) daily for 4 weeks. We examined LE’s effects on superoxide dismutase and catalase- and
glutathione-related enzyme activities, the concentration of lipid peroxides and protein carbonyls,
total oxidant status (TOS) and antioxidant status (TAS), and oxidative stress index (OSI) in the liver,
jejunum, and ileum. We also measured total cholesterol, its biosynthetic precursors (lanosterol,
lathosterol, desmosterol), its degradation products (bile acid precursors) in the serum, liver, jejunum, and ileum, and serum phytosterols (intestinal absorption markers). LE reduced TOS, TAS, and
OSI (p < 0.05) compared with control values, indicating its consistent antioxidant action in all examined organs. LE lowered hepatic desmosterol (p < 0.05) while also reducing 7α- and 24-hydroxycholesterol levels in the liver and ileum (p < 0.01). Serum cholesterol, hepatic gene expression, and the
immunostaining intensity of CYP7A1 were unchanged. In conclusion, LE exerted non-enzymatic
antioxidant effects and reduced cholesterol degradation, reducing its biosynthesis products, thereby
maintaining serum cholesterol levels.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Lemon Flavonoid Extract Eriomin Improves Pro/Antioxidant Status and Interferes with Cholesterol Metabolism without Affecting Serum Cholesterol Levels in Aged Rats
IS  - 10
VL  - 25
DO  - 10.3390/ijms25105221
SP  - 5221
ER  - 

@article{
author = "Šošić-Jurjević, Branka and Borković-Mitić, Slavica and Pavlović, Slađan and Vlahović, Dragana and Miler, Marko and Cesar, Thais and Ajdžanović, Vladimir and Milenković, Dragan and Stellaard, Frans and Trifunović, Svetlana and Filipović, Branko and Dieter, Lutjohann",
year = "2024",
abstract = "This study aimed to assess the antioxidant capacity of lemon flavonoid extract Eriomin®
(LE) and its impact on cholesterol metabolism in the context of healthy aging. We orally treated 24-
month-old male Wistar rats with an LE (40 mg/kg) suspended in 0.3 mL of sunflower oil. At the
same time, control groups received an equal volume of sunflower oil (CON) or remained untreated
(ICON) daily for 4 weeks. We examined LE’s effects on superoxide dismutase and catalase- and
glutathione-related enzyme activities, the concentration of lipid peroxides and protein carbonyls,
total oxidant status (TOS) and antioxidant status (TAS), and oxidative stress index (OSI) in the liver,
jejunum, and ileum. We also measured total cholesterol, its biosynthetic precursors (lanosterol,
lathosterol, desmosterol), its degradation products (bile acid precursors) in the serum, liver, jejunum, and ileum, and serum phytosterols (intestinal absorption markers). LE reduced TOS, TAS, and
OSI (p < 0.05) compared with control values, indicating its consistent antioxidant action in all examined organs. LE lowered hepatic desmosterol (p < 0.05) while also reducing 7α- and 24-hydroxycholesterol levels in the liver and ileum (p < 0.01). Serum cholesterol, hepatic gene expression, and the
immunostaining intensity of CYP7A1 were unchanged. In conclusion, LE exerted non-enzymatic
antioxidant effects and reduced cholesterol degradation, reducing its biosynthesis products, thereby
maintaining serum cholesterol levels.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Lemon Flavonoid Extract Eriomin Improves Pro/Antioxidant Status and Interferes with Cholesterol Metabolism without Affecting Serum Cholesterol Levels in Aged Rats",
number = "10",
volume = "25",
doi = "10.3390/ijms25105221",
pages = "5221"
}

Šošić-Jurjević, B., Borković-Mitić, S., Pavlović, S., Vlahović, D., Miler, M., Cesar, T., Ajdžanović, V., Milenković, D., Stellaard, F., Trifunović, S., Filipović, B.,& Dieter, L.. (2024). Lemon Flavonoid Extract Eriomin Improves Pro/Antioxidant Status and Interferes with Cholesterol Metabolism without Affecting Serum Cholesterol Levels in Aged Rats. in International Journal of Molecular Sciences
Basel: MDPI., 25(10), 5221.
https://doi.org/10.3390/ijms25105221

Šošić-Jurjević B, Borković-Mitić S, Pavlović S, Vlahović D, Miler M, Cesar T, Ajdžanović V, Milenković D, Stellaard F, Trifunović S, Filipović B, Dieter L. Lemon Flavonoid Extract Eriomin Improves Pro/Antioxidant Status and Interferes with Cholesterol Metabolism without Affecting Serum Cholesterol Levels in Aged Rats. in International Journal of Molecular Sciences. 2024;25(10):5221.
doi:10.3390/ijms25105221 .

Šošić-Jurjević, Branka, Borković-Mitić, Slavica, Pavlović, Slađan, Vlahović, Dragana, Miler, Marko, Cesar, Thais, Ajdžanović, Vladimir, Milenković, Dragan, Stellaard, Frans, Trifunović, Svetlana, Filipović, Branko, Dieter, Lutjohann, "Lemon Flavonoid Extract Eriomin Improves Pro/Antioxidant Status and Interferes with Cholesterol Metabolism without Affecting Serum Cholesterol Levels in Aged Rats" in International Journal of Molecular Sciences, 25, no. 10 (2024):5221,
https://doi.org/10.3390/ijms25105221 . .