TY  - JOUR
AU  - Mileski, Ksenija S.
AU  - Ćirić, Ana
AU  - Trifunović, Snežana S.
AU  - Ristić, Mihailo S.
AU  - Soković, Marina
AU  - Matevski, Vlado S.
AU  - Tešević, Vele V.
AU  - Jadranin, Milka B.
AU  - Marin, Petar D.
AU  - Džamić, Ana M.
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6744
AB  - GC-FID and GC-MS were used to determine the chemical composition of the essential oil of the aerial
parts of Heracleum orphanidis Boiss. Also, the HPLC-DAD/ESI-ToF-MS profile of the methanol extracts of
aerial parts and roots was determined. The main components of the essential oil were n-octanol, octyl
hexanoate and n-octyl acetate, while coumarins were the most prevalent compounds in methanol
extracts. An evaluation of antioxidant activity showed that the methanol and aqueous extracts of the aerial
parts had the highest potential. In terms of antimicrobial activity determined by microdilution assays, the
essential oil and methanol extract of the roots showed the greatest effectiveness. The colonies of
Pseudomonas aeruginosa PAO1 treated with H. orphanidis samples produced less toxic pyocyanin,
showed lower twitching and flagella mobility and biofilm formatting was reduced. The analyses in this
study showed the considerable biological potential of H. orphanidis considering free radicals and various
pathogenic strains, including the wild type of P. aeruginosa.
PB  - Royal Society of Chemistry
T2  - Food and Function
T1  - Heracleum orphanidis: chemical characterisation, and comparative evaluation of antioxidant and antimicrobial activities with specific interest in the influence on Pseudomonas aeruginosa PAO1
VL  - 7
DO  - 10.1039/c6fo01018k
SP  - 4061
ER  - 

@article{
author = "Mileski, Ksenija S. and Ćirić, Ana and Trifunović, Snežana S. and Ristić, Mihailo S. and Soković, Marina and Matevski, Vlado S. and Tešević, Vele V. and Jadranin, Milka B. and Marin, Petar D. and Džamić, Ana M.",
year = "2016",
abstract = "GC-FID and GC-MS were used to determine the chemical composition of the essential oil of the aerial
parts of Heracleum orphanidis Boiss. Also, the HPLC-DAD/ESI-ToF-MS profile of the methanol extracts of
aerial parts and roots was determined. The main components of the essential oil were n-octanol, octyl
hexanoate and n-octyl acetate, while coumarins were the most prevalent compounds in methanol
extracts. An evaluation of antioxidant activity showed that the methanol and aqueous extracts of the aerial
parts had the highest potential. In terms of antimicrobial activity determined by microdilution assays, the
essential oil and methanol extract of the roots showed the greatest effectiveness. The colonies of
Pseudomonas aeruginosa PAO1 treated with H. orphanidis samples produced less toxic pyocyanin,
showed lower twitching and flagella mobility and biofilm formatting was reduced. The analyses in this
study showed the considerable biological potential of H. orphanidis considering free radicals and various
pathogenic strains, including the wild type of P. aeruginosa.",
publisher = "Royal Society of Chemistry",
journal = "Food and Function",
title = "Heracleum orphanidis: chemical characterisation, and comparative evaluation of antioxidant and antimicrobial activities with specific interest in the influence on Pseudomonas aeruginosa PAO1",
volume = "7",
doi = "10.1039/c6fo01018k",
pages = "4061"
}

Mileski, K. S., Ćirić, A., Trifunović, S. S., Ristić, M. S., Soković, M., Matevski, V. S., Tešević, V. V., Jadranin, M. B., Marin, P. D.,& Džamić, A. M.. (2016). Heracleum orphanidis: chemical characterisation, and comparative evaluation of antioxidant and antimicrobial activities with specific interest in the influence on Pseudomonas aeruginosa PAO1. in Food and Function
Royal Society of Chemistry., 7, 4061.
https://doi.org/10.1039/c6fo01018k

Mileski KS, Ćirić A, Trifunović SS, Ristić MS, Soković M, Matevski VS, Tešević VV, Jadranin MB, Marin PD, Džamić AM. Heracleum orphanidis: chemical characterisation, and comparative evaluation of antioxidant and antimicrobial activities with specific interest in the influence on Pseudomonas aeruginosa PAO1. in Food and Function. 2016;7:4061.
doi:10.1039/c6fo01018k .

Mileski, Ksenija S., Ćirić, Ana, Trifunović, Snežana S., Ristić, Mihailo S., Soković, Marina, Matevski, Vlado S., Tešević, Vele V., Jadranin, Milka B., Marin, Petar D., Džamić, Ana M., "Heracleum orphanidis: chemical characterisation, and comparative evaluation of antioxidant and antimicrobial activities with specific interest in the influence on Pseudomonas aeruginosa PAO1" in Food and Function, 7 (2016):4061,
https://doi.org/10.1039/c6fo01018k . .

TY  - JOUR
AU  - Džamić, Ana M.
AU  - Nikolic, B. J.
AU  - Giweli, A. A.
AU  - Mitic-Culafic, D. S.
AU  - Soković, Marina
AU  - Ristic, M. S.
AU  - Knežević-Vukčević, Jelena
AU  - Marin, P. D.
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1922
AB  - AimsIn the present work, the Libyan wild-growing Thymus capitatus
   essential oil (EO) was evaluated for its biological properties.
   Methods and ResultsCarvacrol (6819\%) and thymol (1229\%) were found to
   be the main compounds of the oil. Antioxidant properties, determined by
   2,2-diphenylpicrylhydrazyl (DPPH) assay, revealed that IC50 values were
   119, 403 and 105gml(-1) for oil, thymol and carvacrol respectively.
   Microdilution method showed strong antibacterial and especially
   antifungal potential. Tetrazolium (MTT) colorimetric assay indicated
   moderate cytotoxicity towards human cell lines MRC-5, HCT 116 and HT-29
   (IC50=30-150gml(-1)). In adhesion-inhibition assay oil and main
   compounds reduced adhesion of Escherichia coli and Listeria
   monocytogenes on colon cells HT-29 (51 and 39\% of inhibition against
   L.monocytogenes and E.coli respectively).
   ConclusionsEssential oil of Th.capitatus showed moderate cytotoxic
   activity, together with excellent antimicrobial effect, in particular
   against fungi, and significant potential to reduce pathogen colonization
   in colon.
   Significance and Impact of the StudyThis is the first report that EO of
   Th.capitatus could protect against colonization of pathogens to colon
   epithelium. Thymus capitatus from Libya should be recognized as possible
   new source of natural antioxidants, antimicrobials as well as possible
   source of new chemotherapeutics.
T2  - Journal of Applied Microbiology
T1  - Libyan Thymus capitatus essential oil: antioxidant, antimicrobial,
 cytotoxic and colon pathogen adhesion-inhibition properties
IS  - 2
VL  - 119
DO  - 10.1111/jam.12864
SP  - 389
EP  - 399
ER  - 

@article{
author = "Džamić, Ana M. and Nikolic, B. J. and Giweli, A. A. and Mitic-Culafic, D. S. and Soković, Marina and Ristic, M. S. and Knežević-Vukčević, Jelena and Marin, P. D.",
year = "2015",
abstract = "AimsIn the present work, the Libyan wild-growing Thymus capitatus
   essential oil (EO) was evaluated for its biological properties.
   Methods and ResultsCarvacrol (6819\%) and thymol (1229\%) were found to
   be the main compounds of the oil. Antioxidant properties, determined by
   2,2-diphenylpicrylhydrazyl (DPPH) assay, revealed that IC50 values were
   119, 403 and 105gml(-1) for oil, thymol and carvacrol respectively.
   Microdilution method showed strong antibacterial and especially
   antifungal potential. Tetrazolium (MTT) colorimetric assay indicated
   moderate cytotoxicity towards human cell lines MRC-5, HCT 116 and HT-29
   (IC50=30-150gml(-1)). In adhesion-inhibition assay oil and main
   compounds reduced adhesion of Escherichia coli and Listeria
   monocytogenes on colon cells HT-29 (51 and 39\% of inhibition against
   L.monocytogenes and E.coli respectively).
   ConclusionsEssential oil of Th.capitatus showed moderate cytotoxic
   activity, together with excellent antimicrobial effect, in particular
   against fungi, and significant potential to reduce pathogen colonization
   in colon.
   Significance and Impact of the StudyThis is the first report that EO of
   Th.capitatus could protect against colonization of pathogens to colon
   epithelium. Thymus capitatus from Libya should be recognized as possible
   new source of natural antioxidants, antimicrobials as well as possible
   source of new chemotherapeutics.",
journal = "Journal of Applied Microbiology",
title = "Libyan Thymus capitatus essential oil: antioxidant, antimicrobial,
 cytotoxic and colon pathogen adhesion-inhibition properties",
number = "2",
volume = "119",
doi = "10.1111/jam.12864",
pages = "389-399"
}

Džamić, A. M., Nikolic, B. J., Giweli, A. A., Mitic-Culafic, D. S., Soković, M., Ristic, M. S., Knežević-Vukčević, J.,& Marin, P. D.. (2015). Libyan Thymus capitatus essential oil: antioxidant, antimicrobial,
 cytotoxic and colon pathogen adhesion-inhibition properties. in Journal of Applied Microbiology, 119(2), 389-399.
https://doi.org/10.1111/jam.12864

Džamić AM, Nikolic BJ, Giweli AA, Mitic-Culafic DS, Soković M, Ristic MS, Knežević-Vukčević J, Marin PD. Libyan Thymus capitatus essential oil: antioxidant, antimicrobial,
 cytotoxic and colon pathogen adhesion-inhibition properties. in Journal of Applied Microbiology. 2015;119(2):389-399.
doi:10.1111/jam.12864 .

Džamić, Ana M., Nikolic, B. J., Giweli, A. A., Mitic-Culafic, D. S., Soković, Marina, Ristic, M. S., Knežević-Vukčević, Jelena, Marin, P. D., "Libyan Thymus capitatus essential oil: antioxidant, antimicrobial,
 cytotoxic and colon pathogen adhesion-inhibition properties" in Journal of Applied Microbiology, 119, no. 2 (2015):389-399,
https://doi.org/10.1111/jam.12864 . .

TY  - JOUR
AU  - Bukvicki, Danka
AU  - Gottardi, Davide
AU  - Vannini, Lucia
AU  - Džamić, Ana M.
AU  - Ćirić, Ana
AU  - Marin, Petar D.
AU  - Veljic, Milan
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1991
AB  - The chemical composition of the methanolic and ethyl acetate extracts of
   liverwort Lophozia ventricosa (Dicks.) Dumort. was determined by solid
   phase microextraction-gas chromatography mass spectrometry (SPME-GC/MS).
   The dominant compounds in L. ventricosa methanolic extract were o-cymene
   (38.87 \%), beta-barbatene (26.35 \%), and alpha-barbatene (8.30 \%),
   while the main components in ethyl acetate extract were beta-barbatene
   (45.39 \%), maalioxide (9.89 \%), and alpha-barbatene (8.21 \%). The
   antimicrobial activity of L. ventricosa extracts was evaluated against
   15 pathogenic microorganisms using microdilution method. Extracts were
   initially screened against following Gram (-) bacteria Enterobacter
   cloacae, Pseudomonas aeruginosa, Salmonella typhimurium, and Gram (+)
   bacteria: Bacillus cereus, Listeria monocytogenes, Micrococcus flavus,
   and Staphylococcus aureus. The minimum inhibitory concentration (MIC)
   varied from 2.50 to 5.00 mg/mL for bacterial strains, and from 1.25 to
   5.00 mg/mL for micromycetes. For the antifungal bioassays, eight fungi
   were used Aspergillus niger, A. fumigates, A. ochraceus, A. versicolor,
   Penicillium funiculosum, P. ochrochloron, Trichoderma viride, and
   Candida albicans. Sensitivity of fungi to the effect of the extracts was
   higher than that of bacteria tested.
T2  - Brazilian Journal of Botany
T1  - Chemical composition and antimicrobial assessment of liverwort Lophozia
 ventricosa extracts
IS  - 1
VL  - 38
DO  - 10.1007/s40415-014-0104-z
SP  - 25
EP  - 30
ER  - 

@article{
author = "Bukvicki, Danka and Gottardi, Davide and Vannini, Lucia and Džamić, Ana M. and Ćirić, Ana and Marin, Petar D. and Veljic, Milan",
year = "2015",
abstract = "The chemical composition of the methanolic and ethyl acetate extracts of
   liverwort Lophozia ventricosa (Dicks.) Dumort. was determined by solid
   phase microextraction-gas chromatography mass spectrometry (SPME-GC/MS).
   The dominant compounds in L. ventricosa methanolic extract were o-cymene
   (38.87 \%), beta-barbatene (26.35 \%), and alpha-barbatene (8.30 \%),
   while the main components in ethyl acetate extract were beta-barbatene
   (45.39 \%), maalioxide (9.89 \%), and alpha-barbatene (8.21 \%). The
   antimicrobial activity of L. ventricosa extracts was evaluated against
   15 pathogenic microorganisms using microdilution method. Extracts were
   initially screened against following Gram (-) bacteria Enterobacter
   cloacae, Pseudomonas aeruginosa, Salmonella typhimurium, and Gram (+)
   bacteria: Bacillus cereus, Listeria monocytogenes, Micrococcus flavus,
   and Staphylococcus aureus. The minimum inhibitory concentration (MIC)
   varied from 2.50 to 5.00 mg/mL for bacterial strains, and from 1.25 to
   5.00 mg/mL for micromycetes. For the antifungal bioassays, eight fungi
   were used Aspergillus niger, A. fumigates, A. ochraceus, A. versicolor,
   Penicillium funiculosum, P. ochrochloron, Trichoderma viride, and
   Candida albicans. Sensitivity of fungi to the effect of the extracts was
   higher than that of bacteria tested.",
journal = "Brazilian Journal of Botany",
title = "Chemical composition and antimicrobial assessment of liverwort Lophozia
 ventricosa extracts",
number = "1",
volume = "38",
doi = "10.1007/s40415-014-0104-z",
pages = "25-30"
}

Bukvicki, D., Gottardi, D., Vannini, L., Džamić, A. M., Ćirić, A., Marin, P. D.,& Veljic, M.. (2015). Chemical composition and antimicrobial assessment of liverwort Lophozia
 ventricosa extracts. in Brazilian Journal of Botany, 38(1), 25-30.
https://doi.org/10.1007/s40415-014-0104-z

Bukvicki D, Gottardi D, Vannini L, Džamić AM, Ćirić A, Marin PD, Veljic M. Chemical composition and antimicrobial assessment of liverwort Lophozia
 ventricosa extracts. in Brazilian Journal of Botany. 2015;38(1):25-30.
doi:10.1007/s40415-014-0104-z .

Bukvicki, Danka, Gottardi, Davide, Vannini, Lucia, Džamić, Ana M., Ćirić, Ana, Marin, Petar D., Veljic, Milan, "Chemical composition and antimicrobial assessment of liverwort Lophozia
 ventricosa extracts" in Brazilian Journal of Botany, 38, no. 1 (2015):25-30,
https://doi.org/10.1007/s40415-014-0104-z . .

TY  - JOUR
AU  - Mileski, Ksenija S.
AU  - Džamić, Ana M.
AU  - Ćirić, Ana
AU  - Ristic, Mihailo S.
AU  - Grujic, Slavica M.
AU  - Matevski, Vlado S.
AU  - Marin, Petar D.
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2083
AB  - Ferulago macedonica Micevski \& E. Mayer (Apiaceae), an endemic species
   from Macedonia, was evaluated for its essential oil composition and
   biological activity for the first time. The essential oil composition
   from inflorescence and aerial parts, analyzed by gas chromatography and
   gas chromatography-mass spectrometry (GC and GC-MS), indicated a-pinene
   (43.10\% and 22.78\%) and sabinene (26.73\% and 15.46\%) as the main
   components. The antioxidant activities of extracts was determinated
   spectrophotometrically using 2,2-diphenyl-1-picrylhydrazyl (DPPH). The
   strongest activity had methanol extracts of inflorescence and aerial
   parts (IC50= 490 mg/mL and IC50= 630 mg/mL of solution, respectively).
   According to 2,2'-azino-bis(3-ethylbenzothiazoline- 6-sulphonic acid)
   (ABTS) test, the most effective were ethanol and methanol extracts of F.
   macedonica inflorescence (1.69 and 1.63 mg Vit.C/g dw). Total phenolic
   and flavonoid contents evaluation indicated that their amounts were
   ranged between 34.12-59.68 mg gallic acid (GA)/g dw for phenols and
   9.08-23.73 mg quercetin hydrate (QE)/g dw for flavonoids. Antimicrobial
   activity of extracts and essential oils was investigated by micro-well
   dilution assay. Minimum inhibitory (MIC) and minimum
   bactericidal/fungicidal concentrations (MBC/MFC) were defined. The
   methanol extract of inflorescence has shown to be the strongest in both,
   antibacterial (MICs= 4-8 mg/mL; MBCs= 9-13 mg/mL) and antifungal
   activity (MICs= 2-12 mg/mL; MFCs= 6-18 mg/mL). Essential oil from
   inflorescence and aerial parts of F. macedonica manifested stronger
   inhibition on growth of pathogenic bacteria and fungy compared to tested
   extracts and positive standards.
T2  - Records of Natural Products
T1  - Composition, Antimicrobial and Antioxidant Properties of Endemic Species
 Ferulago macedonica Micevski \& E. Mayer
IS  - 2
VL  - 9
SP  - 208
EP  - 223
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_2083
ER  - 

@article{
author = "Mileski, Ksenija S. and Džamić, Ana M. and Ćirić, Ana and Ristic, Mihailo S. and Grujic, Slavica M. and Matevski, Vlado S. and Marin, Petar D.",
year = "2015",
abstract = "Ferulago macedonica Micevski \& E. Mayer (Apiaceae), an endemic species
   from Macedonia, was evaluated for its essential oil composition and
   biological activity for the first time. The essential oil composition
   from inflorescence and aerial parts, analyzed by gas chromatography and
   gas chromatography-mass spectrometry (GC and GC-MS), indicated a-pinene
   (43.10\% and 22.78\%) and sabinene (26.73\% and 15.46\%) as the main
   components. The antioxidant activities of extracts was determinated
   spectrophotometrically using 2,2-diphenyl-1-picrylhydrazyl (DPPH). The
   strongest activity had methanol extracts of inflorescence and aerial
   parts (IC50= 490 mg/mL and IC50= 630 mg/mL of solution, respectively).
   According to 2,2'-azino-bis(3-ethylbenzothiazoline- 6-sulphonic acid)
   (ABTS) test, the most effective were ethanol and methanol extracts of F.
   macedonica inflorescence (1.69 and 1.63 mg Vit.C/g dw). Total phenolic
   and flavonoid contents evaluation indicated that their amounts were
   ranged between 34.12-59.68 mg gallic acid (GA)/g dw for phenols and
   9.08-23.73 mg quercetin hydrate (QE)/g dw for flavonoids. Antimicrobial
   activity of extracts and essential oils was investigated by micro-well
   dilution assay. Minimum inhibitory (MIC) and minimum
   bactericidal/fungicidal concentrations (MBC/MFC) were defined. The
   methanol extract of inflorescence has shown to be the strongest in both,
   antibacterial (MICs= 4-8 mg/mL; MBCs= 9-13 mg/mL) and antifungal
   activity (MICs= 2-12 mg/mL; MFCs= 6-18 mg/mL). Essential oil from
   inflorescence and aerial parts of F. macedonica manifested stronger
   inhibition on growth of pathogenic bacteria and fungy compared to tested
   extracts and positive standards.",
journal = "Records of Natural Products",
title = "Composition, Antimicrobial and Antioxidant Properties of Endemic Species
 Ferulago macedonica Micevski \& E. Mayer",
number = "2",
volume = "9",
pages = "208-223",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_2083"
}

Mileski, K. S., Džamić, A. M., Ćirić, A., Ristic, M. S., Grujic, S. M., Matevski, V. S.,& Marin, P. D.. (2015). Composition, Antimicrobial and Antioxidant Properties of Endemic Species
 Ferulago macedonica Micevski \& E. Mayer. in Records of Natural Products, 9(2), 208-223.
https://hdl.handle.net/21.15107/rcub_ibiss_2083

Mileski KS, Džamić AM, Ćirić A, Ristic MS, Grujic SM, Matevski VS, Marin PD. Composition, Antimicrobial and Antioxidant Properties of Endemic Species
 Ferulago macedonica Micevski \& E. Mayer. in Records of Natural Products. 2015;9(2):208-223.
https://hdl.handle.net/21.15107/rcub_ibiss_2083 .

Mileski, Ksenija S., Džamić, Ana M., Ćirić, Ana, Ristic, Mihailo S., Grujic, Slavica M., Matevski, Vlado S., Marin, Petar D., "Composition, Antimicrobial and Antioxidant Properties of Endemic Species
 Ferulago macedonica Micevski \& E. Mayer" in Records of Natural Products, 9, no. 2 (2015):208-223,
https://hdl.handle.net/21.15107/rcub_ibiss_2083 .

TY  - JOUR
AU  - Mileski, Ksenija
AU  - Džamić, Ana M.
AU  - Ćirić, Ana
AU  - Grujić, Slavica
AU  - Ristić, M.
AU  - Matevski, V.
AU  - Marin, Petar D.
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2304
AB  - This study was undertaken to determine the antioxidant and antimicrobial
   effect of essential oil and extracts of Echinophora sibthorpiana Guss.
   (fam. Apiaceae) collected in Macedonia. The chemical composition of E.
   sibthorpiana essential oil was characterized by the presence of methyl
   eugenol (60.40\%), p-cymene (11.18\%) and a-phellandrene (10.23\%). The
   free radical scavenging activity of extracts and essential oil was
   evaluated by DPPH and ABTS assays. The aqueous extract of aerial parts
   exhibited the strongest scavenging activity (IC50=1.67 mg/ml); results
   of the ABTS test showed that the most effective was the ethanol extract
   of aerial parts (1.11 mg vit. C/g). The essential oil showed stronger
   antioxidant activity compared to hydroxyanisole, ascorbic acid and
   quercetin that were used in the DPPH and ABTS tests, respectively. The
   total phenolic and flavonoid concentrations in the extracts ranged
   between 38.65-60.72 mg GA/g, and 3.15-19.00 mg Qu/g, respectively. The
   antimicrobial properties of the extracts and essential oil were
   investigated using a micro-well dilution technique against human
   pathogenic strains. The results were comparable with the effects of the
   positive controls, streptomycin and fluconazole. These findings indicate
   that E. sibthorpiana extracts and oil can be used in preventive
   treatments and as an alternative for synthetic preservatives.
T2  - Archives of Biological Sciences
T1  - RADICAL SCAVENGING AND ANTIMICROBIAL ACTIVITY OF ESSENTIAL OIL AND
 EXTRACTS OF ECHINOPHORA SIBTHORPIANA GUSS. FROM MACEDONIA
IS  - 1
VL  - 66
DO  - 10.2298/ABS1401401M
SP  - 401
EP  - 413
ER  - 

@article{
author = "Mileski, Ksenija and Džamić, Ana M. and Ćirić, Ana and Grujić, Slavica and Ristić, M. and Matevski, V. and Marin, Petar D.",
year = "2014",
abstract = "This study was undertaken to determine the antioxidant and antimicrobial
   effect of essential oil and extracts of Echinophora sibthorpiana Guss.
   (fam. Apiaceae) collected in Macedonia. The chemical composition of E.
   sibthorpiana essential oil was characterized by the presence of methyl
   eugenol (60.40\%), p-cymene (11.18\%) and a-phellandrene (10.23\%). The
   free radical scavenging activity of extracts and essential oil was
   evaluated by DPPH and ABTS assays. The aqueous extract of aerial parts
   exhibited the strongest scavenging activity (IC50=1.67 mg/ml); results
   of the ABTS test showed that the most effective was the ethanol extract
   of aerial parts (1.11 mg vit. C/g). The essential oil showed stronger
   antioxidant activity compared to hydroxyanisole, ascorbic acid and
   quercetin that were used in the DPPH and ABTS tests, respectively. The
   total phenolic and flavonoid concentrations in the extracts ranged
   between 38.65-60.72 mg GA/g, and 3.15-19.00 mg Qu/g, respectively. The
   antimicrobial properties of the extracts and essential oil were
   investigated using a micro-well dilution technique against human
   pathogenic strains. The results were comparable with the effects of the
   positive controls, streptomycin and fluconazole. These findings indicate
   that E. sibthorpiana extracts and oil can be used in preventive
   treatments and as an alternative for synthetic preservatives.",
journal = "Archives of Biological Sciences",
title = "RADICAL SCAVENGING AND ANTIMICROBIAL ACTIVITY OF ESSENTIAL OIL AND
 EXTRACTS OF ECHINOPHORA SIBTHORPIANA GUSS. FROM MACEDONIA",
number = "1",
volume = "66",
doi = "10.2298/ABS1401401M",
pages = "401-413"
}

Mileski, K., Džamić, A. M., Ćirić, A., Grujić, S., Ristić, M., Matevski, V.,& Marin, P. D.. (2014). RADICAL SCAVENGING AND ANTIMICROBIAL ACTIVITY OF ESSENTIAL OIL AND
 EXTRACTS OF ECHINOPHORA SIBTHORPIANA GUSS. FROM MACEDONIA. in Archives of Biological Sciences, 66(1), 401-413.
https://doi.org/10.2298/ABS1401401M

Mileski K, Džamić AM, Ćirić A, Grujić S, Ristić M, Matevski V, Marin PD. RADICAL SCAVENGING AND ANTIMICROBIAL ACTIVITY OF ESSENTIAL OIL AND
 EXTRACTS OF ECHINOPHORA SIBTHORPIANA GUSS. FROM MACEDONIA. in Archives of Biological Sciences. 2014;66(1):401-413.
doi:10.2298/ABS1401401M .

Mileski, Ksenija, Džamić, Ana M., Ćirić, Ana, Grujić, Slavica, Ristić, M., Matevski, V., Marin, Petar D., "RADICAL SCAVENGING AND ANTIMICROBIAL ACTIVITY OF ESSENTIAL OIL AND
 EXTRACTS OF ECHINOPHORA SIBTHORPIANA GUSS. FROM MACEDONIA" in Archives of Biological Sciences, 66, no. 1 (2014):401-413,
https://doi.org/10.2298/ABS1401401M . .

TY  - JOUR
AU  - Sarac, Zorica
AU  - Matejic, Jelena S.
AU  - Stojanovic-Radic, Zorica Z.
AU  - Veselinovic, Jovana B.
AU  - Džamić, Ana M.
AU  - Bojović, Srđan
AU  - Marin, Petar D.
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2127
AB  - In the current work, in vitro antioxidant, antibacterial, and antifungal
   activites of the needle terpenes of three taxa of Pinus nigra from
   Serbia (ssp. nigra, ssp. pallasiana, and var. banatica) were analyzed.
   The black pine essential oils showed generally weak antioxidative
   properties tested by two methods (DPPH and ABTS scavenging assays),
   where the highest activity was identified in P. nigra var. banatica
   (IC50=25.08 mg/mL and VitC=0.67 mg (vitamin C)/g when tested with the
   DPPH and ABTS reagents, respectively). In the antimicrobial assays, one
   fungal (Aspergilus niger) and two bacterial strains (Staphylococcus
   aureus and Bacillus cereus) showed sensitivity against essential oils of
   all three P. nigra taxa. The tested oils have been shown to possess
   inhibitory action in the range from 20.00 to 0.62 mg/mL, where var.
   banatica exhibited the highest and ssp. nigra the lowest antimicrobial
   action. In order to determine potential compounds that are responsible
   for alternative mode of action, molecular docking simulations inside
   FtsZ (a prokaryotic homolog of tubulin) were performed. Tested compounds
   were the most abundant terpenoid (germacrene D-4-ol) and its
   structurally similar terpene (germacrene D), both present in all three
   essential oils. It was determined that the oxygenated form of the
   molecule creates stable bonds with investigated enzyme FtsZ, and that
   this compound, through this mechanism of action participates in the
   antimicrobial activity. (C) 2014 Elsevier Ltd. All rights reserved.
T2  - Computers in Biology and Medicine
T1  - Biological activity of Pinus nigra terpenes-Evaluation of FtsZ
 inhibition by selected compounds as contribution to their antimicrobial
 activity
VL  - 54
DO  - 10.1016/j.compbiomed.2014.08.022
SP  - 72
EP  - 78
ER  - 

@article{
author = "Sarac, Zorica and Matejic, Jelena S. and Stojanovic-Radic, Zorica Z. and Veselinovic, Jovana B. and Džamić, Ana M. and Bojović, Srđan and Marin, Petar D.",
year = "2014",
abstract = "In the current work, in vitro antioxidant, antibacterial, and antifungal
   activites of the needle terpenes of three taxa of Pinus nigra from
   Serbia (ssp. nigra, ssp. pallasiana, and var. banatica) were analyzed.
   The black pine essential oils showed generally weak antioxidative
   properties tested by two methods (DPPH and ABTS scavenging assays),
   where the highest activity was identified in P. nigra var. banatica
   (IC50=25.08 mg/mL and VitC=0.67 mg (vitamin C)/g when tested with the
   DPPH and ABTS reagents, respectively). In the antimicrobial assays, one
   fungal (Aspergilus niger) and two bacterial strains (Staphylococcus
   aureus and Bacillus cereus) showed sensitivity against essential oils of
   all three P. nigra taxa. The tested oils have been shown to possess
   inhibitory action in the range from 20.00 to 0.62 mg/mL, where var.
   banatica exhibited the highest and ssp. nigra the lowest antimicrobial
   action. In order to determine potential compounds that are responsible
   for alternative mode of action, molecular docking simulations inside
   FtsZ (a prokaryotic homolog of tubulin) were performed. Tested compounds
   were the most abundant terpenoid (germacrene D-4-ol) and its
   structurally similar terpene (germacrene D), both present in all three
   essential oils. It was determined that the oxygenated form of the
   molecule creates stable bonds with investigated enzyme FtsZ, and that
   this compound, through this mechanism of action participates in the
   antimicrobial activity. (C) 2014 Elsevier Ltd. All rights reserved.",
journal = "Computers in Biology and Medicine",
title = "Biological activity of Pinus nigra terpenes-Evaluation of FtsZ
 inhibition by selected compounds as contribution to their antimicrobial
 activity",
volume = "54",
doi = "10.1016/j.compbiomed.2014.08.022",
pages = "72-78"
}

Sarac, Z., Matejic, J. S., Stojanovic-Radic, Z. Z., Veselinovic, J. B., Džamić, A. M., Bojović, S.,& Marin, P. D.. (2014). Biological activity of Pinus nigra terpenes-Evaluation of FtsZ
 inhibition by selected compounds as contribution to their antimicrobial
 activity. in Computers in Biology and Medicine, 54, 72-78.
https://doi.org/10.1016/j.compbiomed.2014.08.022

Sarac Z, Matejic JS, Stojanovic-Radic ZZ, Veselinovic JB, Džamić AM, Bojović S, Marin PD. Biological activity of Pinus nigra terpenes-Evaluation of FtsZ
 inhibition by selected compounds as contribution to their antimicrobial
 activity. in Computers in Biology and Medicine. 2014;54:72-78.
doi:10.1016/j.compbiomed.2014.08.022 .

Sarac, Zorica, Matejic, Jelena S., Stojanovic-Radic, Zorica Z., Veselinovic, Jovana B., Džamić, Ana M., Bojović, Srđan, Marin, Petar D., "Biological activity of Pinus nigra terpenes-Evaluation of FtsZ
 inhibition by selected compounds as contribution to their antimicrobial
 activity" in Computers in Biology and Medicine, 54 (2014):72-78,
https://doi.org/10.1016/j.compbiomed.2014.08.022 . .

TY  - JOUR
AU  - Giweli, Abdulhamid A.
AU  - Džamić, Ana M.
AU  - Soković, Marina
AU  - Ristić, M.S.
AU  - Janaćković, P.
AU  - Marin, P.D.
PY  - 2013
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/330
AB  - The composition of essential oil isolated from Salvia fruticosa, wild growing in Libya, was analyzed by GC and GC-MS. Forty-five compounds could be identified. The essential oil contained 1,8-cineole (49.34%), camphor (7.53%), β-pinene (7.38%), myrcene (7.38%), α-pinene (5.15%), β-caryophyllene (4.13%) and α-terpineol (3.25). Antioxidant activity was analyzed using the DPP H free radical scavenging method and low antioxidant activity was found (IC50 = 15.53 mg/ml). The oil was also screened for its antimicrobial activity against eight bacteria (four Gram-negative and four Gram-positive) and eight fungi. The essential oil of S. fruticosa showed minimal inhibitory activity (MIC) at 0.125-1.5 mg/ml and bactericidal (MBC) at 0.5-2.0 mg/ml. In addition, it exhibited fungistatic (MIC) at 0.125-1.0 mg/ml and fungicidal effect (MFC) at 0.125-1.5 mg/ml.
T2  - Archives of Biological Sciences
T1  - The chemical composition, antimicrobial and antioxidant activities of the essential oil of Salvia fruticosa growing wild in Libya
IS  - 1
VL  - 65
SP  - 321
EP  - 329
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_330
ER  - 

@article{
author = "Giweli, Abdulhamid A. and Džamić, Ana M. and Soković, Marina and Ristić, M.S. and Janaćković, P. and Marin, P.D.",
year = "2013, 2013",
abstract = "The composition of essential oil isolated from Salvia fruticosa, wild growing in Libya, was analyzed by GC and GC-MS. Forty-five compounds could be identified. The essential oil contained 1,8-cineole (49.34%), camphor (7.53%), β-pinene (7.38%), myrcene (7.38%), α-pinene (5.15%), β-caryophyllene (4.13%) and α-terpineol (3.25). Antioxidant activity was analyzed using the DPP H free radical scavenging method and low antioxidant activity was found (IC50 = 15.53 mg/ml). The oil was also screened for its antimicrobial activity against eight bacteria (four Gram-negative and four Gram-positive) and eight fungi. The essential oil of S. fruticosa showed minimal inhibitory activity (MIC) at 0.125-1.5 mg/ml and bactericidal (MBC) at 0.5-2.0 mg/ml. In addition, it exhibited fungistatic (MIC) at 0.125-1.0 mg/ml and fungicidal effect (MFC) at 0.125-1.5 mg/ml.",
journal = "Archives of Biological Sciences",
title = "The chemical composition, antimicrobial and antioxidant activities of the essential oil of Salvia fruticosa growing wild in Libya",
number = "1",
volume = "65",
pages = "321-329",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_330"
}

Giweli, A. A., Džamić, A. M., Soković, M., Ristić, M.S., Janaćković, P.,& Marin, P.D.. (2013). The chemical composition, antimicrobial and antioxidant activities of the essential oil of Salvia fruticosa growing wild in Libya. in Archives of Biological Sciences, 65(1), 321-329.
https://hdl.handle.net/21.15107/rcub_ibiss_330

Giweli AA, Džamić AM, Soković M, Ristić M, Janaćković P, Marin P. The chemical composition, antimicrobial and antioxidant activities of the essential oil of Salvia fruticosa growing wild in Libya. in Archives of Biological Sciences. 2013;65(1):321-329.
https://hdl.handle.net/21.15107/rcub_ibiss_330 .

Giweli, Abdulhamid A., Džamić, Ana M., Soković, Marina, Ristić, M.S., Janaćković, P., Marin, P.D., "The chemical composition, antimicrobial and antioxidant activities of the essential oil of Salvia fruticosa growing wild in Libya" in Archives of Biological Sciences, 65, no. 1 (2013):321-329,
https://hdl.handle.net/21.15107/rcub_ibiss_330 .

TY  - JOUR
AU  - Džamić, Ana M.
AU  - Soković, Marina
AU  - Ristić, Mihailo S.
AU  - Novaković, Miroslav
AU  - Grujić-Jovanović, Slavica M.
AU  - Tešević, Vele
AU  - Marin, Petar D.
PY  - 2010
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/517
AB  - The present study describes the antifungal and antioxidant activity of Mentha longifolia (L.) Hudson essential oil. This plant is native to Europe, Central Asia and Australia. It is used as carminative, stomachic and stimulant and also in aromatherapy. The essential oil profile was determined by GC and GC-MS. The main compounds in the oil were trans-dihydrocarvone (23.64%), piperitone (17.33%) and cis-dihydrocarvone (15.68%). Minimum inhibitory concentrations (MIC) and minimum fungicidal concentrations (MFC) were recorded using the microdilution method. Commercial antimicotic bifonazol was used as a control. The concentration of 10 μl/ml showed fungicidal activity against Aspergillus and Fusarium species, Penicillium funiculosum and Trichoderma viride. Concentration of 5 μl/ml was efficient against Trichophyton menthagrophytes and yeast Candida albicans. The most sensitive micromycetes were Cladosporium fulvum, C. cladosporium cladosporioides and Penicillium ochrochloron where concentration of 2.5 μl/ml was lethal. The antioxidant activity of essential oil was evaluated by means of the 2,2-diphenyl-1-picrylhydrazil (DPPH) radical scavenging method. The essential oil of M. longifolia was able to reduce DPPH radicals into the DPPH-H form, and this activity was dose-dependent. The oil exhibited significant potential for antioxidant activity, and reduced DPPH to 50% (IC50=0,659 ml/ml of solution). .
AB  - U radu je ispitivano potencijalno delovanje etarskog ulja Mentha longifolia (L.) Huds. kao antifungalnog i antioksidativnog agensa. Vrsta M. longifolia je samonikla u Evropi, Centralnoj Aziji i Australiji. Koristi se kao karminativ, stomahik i stimulant u aromaterapiji. Kompozicija etarskog ulja je određivana korišćenjem gasne hromatografi je (GH) i gasne hromatografije sa masenom spektroskopijom (GH-MS). Dominantne komponente u ispitivanom etarskom ulju su: trans-dihidrokarvon (23.64%), piperiton (17.33%) i cis-dihidrokarvon (15.68%). Antifungalna aktivnost je ispitivana metodom mikrodilucije i određivane su minimalne inhibitorne i fungicidne koncentracije ulja i komercijalnog fungicida bifonazola. Etarsko ulje je pokazalo fungicidno dejstvo pri koncentraciji 10 μl/ml za gljive iz rodova Aspergillus i Fusarium kao i za vrste Penicillium funiculosum i Trichoderma viride. Najosetljivije su mikromicete Cladosporium fulvum, C. cladosporioides i Penicillium ochrochloron, za koje je letalna koncentracija 2.5 μl/ml. Antioksidativna aktivnost je rađena korišćenjem DPPH kao hvatača slobodnih radikala. Etarsko ulje M. longifolia je pokazalo sposobnost redukcije DPPH radikala u DPPH-H formu. Antifungalna aktivnost je predstavljena redukcijom DPPH na 50% (IC50=0,659 ml/ml rastvora).
T2  - Botanica Serbica
T1  - Antifungalna i antioksidativna aktivnost etarskog ulja Mentha longifolia (L.) Huds. (Lamiaceae)
T1  - Antifungal and antioxidant activity of Mentha longifolia (L.) Hudson (Lamiaceae) essential oil
IS  - 1
VL  - 34
SP  - 57
EP  - 61
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_517
ER  - 

@article{
author = "Džamić, Ana M. and Soković, Marina and Ristić, Mihailo S. and Novaković, Miroslav and Grujić-Jovanović, Slavica M. and Tešević, Vele and Marin, Petar D.",
year = "2010, 2010",
abstract = "The present study describes the antifungal and antioxidant activity of Mentha longifolia (L.) Hudson essential oil. This plant is native to Europe, Central Asia and Australia. It is used as carminative, stomachic and stimulant and also in aromatherapy. The essential oil profile was determined by GC and GC-MS. The main compounds in the oil were trans-dihydrocarvone (23.64%), piperitone (17.33%) and cis-dihydrocarvone (15.68%). Minimum inhibitory concentrations (MIC) and minimum fungicidal concentrations (MFC) were recorded using the microdilution method. Commercial antimicotic bifonazol was used as a control. The concentration of 10 μl/ml showed fungicidal activity against Aspergillus and Fusarium species, Penicillium funiculosum and Trichoderma viride. Concentration of 5 μl/ml was efficient against Trichophyton menthagrophytes and yeast Candida albicans. The most sensitive micromycetes were Cladosporium fulvum, C. cladosporium cladosporioides and Penicillium ochrochloron where concentration of 2.5 μl/ml was lethal. The antioxidant activity of essential oil was evaluated by means of the 2,2-diphenyl-1-picrylhydrazil (DPPH) radical scavenging method. The essential oil of M. longifolia was able to reduce DPPH radicals into the DPPH-H form, and this activity was dose-dependent. The oil exhibited significant potential for antioxidant activity, and reduced DPPH to 50% (IC50=0,659 ml/ml of solution). ., U radu je ispitivano potencijalno delovanje etarskog ulja Mentha longifolia (L.) Huds. kao antifungalnog i antioksidativnog agensa. Vrsta M. longifolia je samonikla u Evropi, Centralnoj Aziji i Australiji. Koristi se kao karminativ, stomahik i stimulant u aromaterapiji. Kompozicija etarskog ulja je određivana korišćenjem gasne hromatografi je (GH) i gasne hromatografije sa masenom spektroskopijom (GH-MS). Dominantne komponente u ispitivanom etarskom ulju su: trans-dihidrokarvon (23.64%), piperiton (17.33%) i cis-dihidrokarvon (15.68%). Antifungalna aktivnost je ispitivana metodom mikrodilucije i određivane su minimalne inhibitorne i fungicidne koncentracije ulja i komercijalnog fungicida bifonazola. Etarsko ulje je pokazalo fungicidno dejstvo pri koncentraciji 10 μl/ml za gljive iz rodova Aspergillus i Fusarium kao i za vrste Penicillium funiculosum i Trichoderma viride. Najosetljivije su mikromicete Cladosporium fulvum, C. cladosporioides i Penicillium ochrochloron, za koje je letalna koncentracija 2.5 μl/ml. Antioksidativna aktivnost je rađena korišćenjem DPPH kao hvatača slobodnih radikala. Etarsko ulje M. longifolia je pokazalo sposobnost redukcije DPPH radikala u DPPH-H formu. Antifungalna aktivnost je predstavljena redukcijom DPPH na 50% (IC50=0,659 ml/ml rastvora).",
journal = "Botanica Serbica",
title = "Antifungalna i antioksidativna aktivnost etarskog ulja Mentha longifolia (L.) Huds. (Lamiaceae), Antifungal and antioxidant activity of Mentha longifolia (L.) Hudson (Lamiaceae) essential oil",
number = "1",
volume = "34",
pages = "57-61",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_517"
}

Džamić, A. M., Soković, M., Ristić, M. S., Novaković, M., Grujić-Jovanović, S. M., Tešević, V.,& Marin, P. D.. (2010). Antifungalna i antioksidativna aktivnost etarskog ulja Mentha longifolia (L.) Huds. (Lamiaceae). in Botanica Serbica, 34(1), 57-61.
https://hdl.handle.net/21.15107/rcub_ibiss_517

Džamić AM, Soković M, Ristić MS, Novaković M, Grujić-Jovanović SM, Tešević V, Marin PD. Antifungalna i antioksidativna aktivnost etarskog ulja Mentha longifolia (L.) Huds. (Lamiaceae). in Botanica Serbica. 2010;34(1):57-61.
https://hdl.handle.net/21.15107/rcub_ibiss_517 .

Džamić, Ana M., Soković, Marina, Ristić, Mihailo S., Novaković, Miroslav, Grujić-Jovanović, Slavica M., Tešević, Vele, Marin, Petar D., "Antifungalna i antioksidativna aktivnost etarskog ulja Mentha longifolia (L.) Huds. (Lamiaceae)" in Botanica Serbica, 34, no. 1 (2010):57-61,
https://hdl.handle.net/21.15107/rcub_ibiss_517 .