TY  - JOUR
AU  - Marković, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Radulović, Olga
AU  - Paunović, Danijela
AU  - Antonić Reljin, Dragana
AU  - Jevremović, Slađana
AU  - Uzelac, Branka
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6522
AB  - The genus Fritillaria is characterized by the production of alkaloids, primarily of the isosteroidal type. The aim of this preliminary study was to perform a histolocalization screening aimed at revealing the presence of alkaloids in the bulbs of F. meleagris, cultured in vitro under different growth regimes and at different developmental stages, as a powerful tool to identify preferable in vitro plant material for alkaloid isolation. Histochemical localization of alkaloids was performed using Wagner’s and Dragendorff’s reagents in fresh sections of bulbs cultured at 24 °C or 7 °C for 4 weeks, as well as those cultured at 24 °C following the 4-week chilling treatment, which were sampled at the beginning of sprouting. A positive reaction was observed with both reagents and was particularly intense in cold-treated bulbs. Alkaloids were mainly distributed in the bulbs and partly in the sprouts of the in vitro grown F. meleagris. The most intense staining, indicative of high alkaloid content, was observed in the bulb scales of pre-chilled bulbs that sprouted at 24 °C, rendering them preferable as in vitro plant material for alkaloid isolation. The results suggest that alkaloid production in the bulbs of F. meleagris can be improved by manipulating growth in the microenvironment of in vitro cultures, in order to meet the increasing industrial demand for medicinally or commercially important metabolites used as traditional medicines and herbal remedies.
PB  - Basel : MDPI
T2  - Horticulturae
T1  - Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime
IS  - 1
VL  - 10
DO  - 10.3390/horticulturae10010017
SP  - 17
ER  - 

@article{
author = "Marković, Marija and Trifunović-Momčilov, Milana and Radulović, Olga and Paunović, Danijela and Antonić Reljin, Dragana and Jevremović, Slađana and Uzelac, Branka",
year = "2024",
abstract = "The genus Fritillaria is characterized by the production of alkaloids, primarily of the isosteroidal type. The aim of this preliminary study was to perform a histolocalization screening aimed at revealing the presence of alkaloids in the bulbs of F. meleagris, cultured in vitro under different growth regimes and at different developmental stages, as a powerful tool to identify preferable in vitro plant material for alkaloid isolation. Histochemical localization of alkaloids was performed using Wagner’s and Dragendorff’s reagents in fresh sections of bulbs cultured at 24 °C or 7 °C for 4 weeks, as well as those cultured at 24 °C following the 4-week chilling treatment, which were sampled at the beginning of sprouting. A positive reaction was observed with both reagents and was particularly intense in cold-treated bulbs. Alkaloids were mainly distributed in the bulbs and partly in the sprouts of the in vitro grown F. meleagris. The most intense staining, indicative of high alkaloid content, was observed in the bulb scales of pre-chilled bulbs that sprouted at 24 °C, rendering them preferable as in vitro plant material for alkaloid isolation. The results suggest that alkaloid production in the bulbs of F. meleagris can be improved by manipulating growth in the microenvironment of in vitro cultures, in order to meet the increasing industrial demand for medicinally or commercially important metabolites used as traditional medicines and herbal remedies.",
publisher = "Basel : MDPI",
journal = "Horticulturae",
title = "Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime",
number = "1",
volume = "10",
doi = "10.3390/horticulturae10010017",
pages = "17"
}

Marković, M., Trifunović-Momčilov, M., Radulović, O., Paunović, D., Antonić Reljin, D., Jevremović, S.,& Uzelac, B.. (2024). Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime. in Horticulturae
Basel : MDPI., 10(1), 17.
https://doi.org/10.3390/horticulturae10010017

Marković M, Trifunović-Momčilov M, Radulović O, Paunović D, Antonić Reljin D, Jevremović S, Uzelac B. Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime. in Horticulturae. 2024;10(1):17.
doi:10.3390/horticulturae10010017 .

Marković, Marija, Trifunović-Momčilov, Milana, Radulović, Olga, Paunović, Danijela, Antonić Reljin, Dragana, Jevremović, Slađana, Uzelac, Branka, "Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime" in Horticulturae, 10, no. 1 (2024):17,
https://doi.org/10.3390/horticulturae10010017 . .

TY  - JOUR
AU  - Popržen, Tatjana
AU  - Nikolić, Ivan
AU  - Krstić-Milošević, Dijana
AU  - Uzelac, Branka
AU  - Trifunović-Momčilov, Milana
AU  - Marković, Marija
AU  - Radulović, Olga
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6442
AB  - The rhizosphere represents a center of complex and dynamic interactions between plants and microbes, resulting in various positive effects on plant growth and development. However, less is known about the effects of indole-3-acetic acid (IAA) on aquatic plants. In this study, we report the characterization of four Pseudomonas strains isolated from the rhizosphere of the common duckweed (Lemna minor) with IAA-degradation and -utilization ability. Our results confirm previous reports on the negative effect of IAA on aquatic plants, contrary to the effect on terrestrial plants. P. putida A3-104/5 demonstrated particularly beneficial traits, as it exhibited not only IAA-degrading and -producing activity but also a positive effect on the doubling time of duckweeds in the presence of IAA, positive chemotaxis in the presence of IAA, increased tolerance to oxidative stress in the presence of IAA and increased biofilm formation related to IAA. Similarly, P. gessardii C31-106/3 significantly shortened the doubling time of duckweeds in the presence of IAA, while having a neutral effect in the absence of IAA. These traits are important in the context of plant–bacteria interactions and highlight the role of IAA as a common metabolite in these interactions, especially in aquatic environments where plants are facing unique challenges compared to their terrestrial counterparts. We conclude that IAA-degrading and -producing strains presented in this study might regulate IAA effects on aquatic plants and confer evolutionary benefits under adverse conditions (e.g., under oxidative stress, excess of IAA or nutrient scarcity).
PB  - Basel : MDPI
T2  - International Journal of Molecular Sciences
T1  - Characterization of the IAA-producing and -degrading Pseudomonas strains Regulating Growth of the Common Duckweed (Lemna minor L.)
IS  - 24
VL  - 24
DO  - 10.3390/ijms242417207
SP  - 17207
ER  - 

@article{
author = "Popržen, Tatjana and Nikolić, Ivan and Krstić-Milošević, Dijana and Uzelac, Branka and Trifunović-Momčilov, Milana and Marković, Marija and Radulović, Olga",
year = "2023",
abstract = "The rhizosphere represents a center of complex and dynamic interactions between plants and microbes, resulting in various positive effects on plant growth and development. However, less is known about the effects of indole-3-acetic acid (IAA) on aquatic plants. In this study, we report the characterization of four Pseudomonas strains isolated from the rhizosphere of the common duckweed (Lemna minor) with IAA-degradation and -utilization ability. Our results confirm previous reports on the negative effect of IAA on aquatic plants, contrary to the effect on terrestrial plants. P. putida A3-104/5 demonstrated particularly beneficial traits, as it exhibited not only IAA-degrading and -producing activity but also a positive effect on the doubling time of duckweeds in the presence of IAA, positive chemotaxis in the presence of IAA, increased tolerance to oxidative stress in the presence of IAA and increased biofilm formation related to IAA. Similarly, P. gessardii C31-106/3 significantly shortened the doubling time of duckweeds in the presence of IAA, while having a neutral effect in the absence of IAA. These traits are important in the context of plant–bacteria interactions and highlight the role of IAA as a common metabolite in these interactions, especially in aquatic environments where plants are facing unique challenges compared to their terrestrial counterparts. We conclude that IAA-degrading and -producing strains presented in this study might regulate IAA effects on aquatic plants and confer evolutionary benefits under adverse conditions (e.g., under oxidative stress, excess of IAA or nutrient scarcity).",
publisher = "Basel : MDPI",
journal = "International Journal of Molecular Sciences",
title = "Characterization of the IAA-producing and -degrading Pseudomonas strains Regulating Growth of the Common Duckweed (Lemna minor L.)",
number = "24",
volume = "24",
doi = "10.3390/ijms242417207",
pages = "17207"
}

Popržen, T., Nikolić, I., Krstić-Milošević, D., Uzelac, B., Trifunović-Momčilov, M., Marković, M.,& Radulović, O.. (2023). Characterization of the IAA-producing and -degrading Pseudomonas strains Regulating Growth of the Common Duckweed (Lemna minor L.). in International Journal of Molecular Sciences
Basel : MDPI., 24(24), 17207.
https://doi.org/10.3390/ijms242417207

Popržen T, Nikolić I, Krstić-Milošević D, Uzelac B, Trifunović-Momčilov M, Marković M, Radulović O. Characterization of the IAA-producing and -degrading Pseudomonas strains Regulating Growth of the Common Duckweed (Lemna minor L.). in International Journal of Molecular Sciences. 2023;24(24):17207.
doi:10.3390/ijms242417207 .

Popržen, Tatjana, Nikolić, Ivan, Krstić-Milošević, Dijana, Uzelac, Branka, Trifunović-Momčilov, Milana, Marković, Marija, Radulović, Olga, "Characterization of the IAA-producing and -degrading Pseudomonas strains Regulating Growth of the Common Duckweed (Lemna minor L.)" in International Journal of Molecular Sciences, 24, no. 24 (2023):17207,
https://doi.org/10.3390/ijms242417207 . .

TY  - JOUR
AU  - Đurić, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
AU  - Marković, Marija
AU  - Radulović, Olga
AU  - Subotić, Angelina
AU  - Uzelac, Branka
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6135
AB  - Impatiens walleriana is a decorative horticultural plant species. Commercial production requires that the plants be brought to market, often accompanied by reduced water content during transport. Drought significantly affects metabolic processes in plants. The effects of polyethylene glycol (PEG)-induced water deficit on shoots of I. walleriana were investigated using different mechanisms at the biochemical level. In addition, the potential water deficit-ameliorating effect of sodium nitroprusside (SNP) was tested. Shoots of I. walleriana were initially grown on MS media supplemented with SNP (50, 100 and 250 μM). After pre-treatments, shoots were further transferred to media supplemented with PEG8000 (3%) and/or SNP (50, 100 and 250 μM). Water deficit conditions increased proline, photosynthetic pigments, malondialdehyde (MDA), H2O2, total phenolic content and antioxidant activity. In addition, PEG-induced water deficit increased superoxide dismutase (SOD) and peroxidase (POX) activities but decreased catalase (CAT) activity. SNP did not significantly affect photosynthetic pigments and total phenolic content but increased proline accumulation, MDA and H2O2 content, especially when applied simultaneously with PEG. Moreover, none of the investigated SNP pretreatments significantly altered the activities of SOD, POX, and CAT in I. walleriana. The results indicate that exogenous application of SNP effectively alleviated water deficit stress in shoots of I. walleriana grown in vitro.
PB  - Basel: MDPI
T2  - Agriculture
T1  - Does Sodium Nitroprusside Alleviate Water Deficit Stress in Impatiens walleriana Shoots Grown In Vitro?
IS  - 10
VL  - 13
DO  - 10.3390/agriculture13101903
SP  - 1903
ER  - 

@article{
author = "Đurić, Marija and Trifunović-Momčilov, Milana and Milošević, Snežana and Marković, Marija and Radulović, Olga and Subotić, Angelina and Uzelac, Branka",
year = "2023",
abstract = "Impatiens walleriana is a decorative horticultural plant species. Commercial production requires that the plants be brought to market, often accompanied by reduced water content during transport. Drought significantly affects metabolic processes in plants. The effects of polyethylene glycol (PEG)-induced water deficit on shoots of I. walleriana were investigated using different mechanisms at the biochemical level. In addition, the potential water deficit-ameliorating effect of sodium nitroprusside (SNP) was tested. Shoots of I. walleriana were initially grown on MS media supplemented with SNP (50, 100 and 250 μM). After pre-treatments, shoots were further transferred to media supplemented with PEG8000 (3%) and/or SNP (50, 100 and 250 μM). Water deficit conditions increased proline, photosynthetic pigments, malondialdehyde (MDA), H2O2, total phenolic content and antioxidant activity. In addition, PEG-induced water deficit increased superoxide dismutase (SOD) and peroxidase (POX) activities but decreased catalase (CAT) activity. SNP did not significantly affect photosynthetic pigments and total phenolic content but increased proline accumulation, MDA and H2O2 content, especially when applied simultaneously with PEG. Moreover, none of the investigated SNP pretreatments significantly altered the activities of SOD, POX, and CAT in I. walleriana. The results indicate that exogenous application of SNP effectively alleviated water deficit stress in shoots of I. walleriana grown in vitro.",
publisher = "Basel: MDPI",
journal = "Agriculture",
title = "Does Sodium Nitroprusside Alleviate Water Deficit Stress in Impatiens walleriana Shoots Grown In Vitro?",
number = "10",
volume = "13",
doi = "10.3390/agriculture13101903",
pages = "1903"
}

Đurić, M., Trifunović-Momčilov, M., Milošević, S., Marković, M., Radulović, O., Subotić, A.,& Uzelac, B.. (2023). Does Sodium Nitroprusside Alleviate Water Deficit Stress in Impatiens walleriana Shoots Grown In Vitro?. in Agriculture
Basel: MDPI., 13(10), 1903.
https://doi.org/10.3390/agriculture13101903

Đurić M, Trifunović-Momčilov M, Milošević S, Marković M, Radulović O, Subotić A, Uzelac B. Does Sodium Nitroprusside Alleviate Water Deficit Stress in Impatiens walleriana Shoots Grown In Vitro?. in Agriculture. 2023;13(10):1903.
doi:10.3390/agriculture13101903 .

Đurić, Marija, Trifunović-Momčilov, Milana, Milošević, Snežana, Marković, Marija, Radulović, Olga, Subotić, Angelina, Uzelac, Branka, "Does Sodium Nitroprusside Alleviate Water Deficit Stress in Impatiens walleriana Shoots Grown In Vitro?" in Agriculture, 13, no. 10 (2023):1903,
https://doi.org/10.3390/agriculture13101903 . .

TY  - JOUR
AU  - Marković, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Radulović, Olga
AU  - Paunović, Danijela
AU  - Antonić Reljin, Dragana
AU  - Uzelac, Branka
AU  - Subotić, Angelina
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6089
AB  - Fritillaria meleagris is a horticulturally and medicinally valuable bulbous plant that requires a period of low temperatures for proper growth and flowering. Since conventional methods of propagation are ineffective and very slow, tissue culture techniques offer an integrated approach to mass production of this valuable geophyte. In this study, we investigated the effects of various auxin–cytokinin combinations on different morphogenetic pathways in bulb scale culture. Bulbs obtained in vitro were cut longitudinally, and bulb scales were cultured for four weeks at 7 °C on MS medium supplemented with 6-benzylaminopurine (BAP) in combination with 2,4-dichlorophenoxyacetic acid (2,4-D) or α-naphthaleneacetic acid (NAA) at different concentrations in order to investigate the influence of plant growth regulators (PGRs) on different morphogenetic responses. Regeneration percentage, number of shoots per explant, shoot length, number of bulbs and number of somatic embryos were monitored weekly. After chilling, bulb scales were transferred to 24 °C, and all parameters were recorded again. Low PGR concentrations were very effective for shoot multiplication, yielding up to 5.5 shoots per explant. 2,4-D (at 2 mg/L) in combination with low BAP (0.25 mg/L) produced the highest number of bulbs (11.00 ± 0.00), while PGR-free medium was extremely effective in somatic embryo formation (13.50 ± 2.90). Detached somatic embryos and bulblets continued to grow and develop on fresh PGR-free medium. We present data demonstrating that low auxin–cytokinin concentrations and PGR-free medium provide an effective method for a combined morphogenetic pathway in F. meleagris that is suitable for large-scale propagation.
PB  - Basel : MDPI
T2  - Horticulturae
T1  - The Effects of Different Auxin–Cytokinin Combinations on Morphogenesis of Fritillaria meleagris Using Bulb Scale Sections In Vitro
IS  - 8
VL  - 9
DO  - 10.3390/horticulturae9080910
SP  - 910
ER  - 

@article{
author = "Marković, Marija and Trifunović-Momčilov, Milana and Radulović, Olga and Paunović, Danijela and Antonić Reljin, Dragana and Uzelac, Branka and Subotić, Angelina",
year = "2023",
abstract = "Fritillaria meleagris is a horticulturally and medicinally valuable bulbous plant that requires a period of low temperatures for proper growth and flowering. Since conventional methods of propagation are ineffective and very slow, tissue culture techniques offer an integrated approach to mass production of this valuable geophyte. In this study, we investigated the effects of various auxin–cytokinin combinations on different morphogenetic pathways in bulb scale culture. Bulbs obtained in vitro were cut longitudinally, and bulb scales were cultured for four weeks at 7 °C on MS medium supplemented with 6-benzylaminopurine (BAP) in combination with 2,4-dichlorophenoxyacetic acid (2,4-D) or α-naphthaleneacetic acid (NAA) at different concentrations in order to investigate the influence of plant growth regulators (PGRs) on different morphogenetic responses. Regeneration percentage, number of shoots per explant, shoot length, number of bulbs and number of somatic embryos were monitored weekly. After chilling, bulb scales were transferred to 24 °C, and all parameters were recorded again. Low PGR concentrations were very effective for shoot multiplication, yielding up to 5.5 shoots per explant. 2,4-D (at 2 mg/L) in combination with low BAP (0.25 mg/L) produced the highest number of bulbs (11.00 ± 0.00), while PGR-free medium was extremely effective in somatic embryo formation (13.50 ± 2.90). Detached somatic embryos and bulblets continued to grow and develop on fresh PGR-free medium. We present data demonstrating that low auxin–cytokinin concentrations and PGR-free medium provide an effective method for a combined morphogenetic pathway in F. meleagris that is suitable for large-scale propagation.",
publisher = "Basel : MDPI",
journal = "Horticulturae",
title = "The Effects of Different Auxin–Cytokinin Combinations on Morphogenesis of Fritillaria meleagris Using Bulb Scale Sections In Vitro",
number = "8",
volume = "9",
doi = "10.3390/horticulturae9080910",
pages = "910"
}

Marković, M., Trifunović-Momčilov, M., Radulović, O., Paunović, D., Antonić Reljin, D., Uzelac, B.,& Subotić, A.. (2023). The Effects of Different Auxin–Cytokinin Combinations on Morphogenesis of Fritillaria meleagris Using Bulb Scale Sections In Vitro. in Horticulturae
Basel : MDPI., 9(8), 910.
https://doi.org/10.3390/horticulturae9080910

Marković M, Trifunović-Momčilov M, Radulović O, Paunović D, Antonić Reljin D, Uzelac B, Subotić A. The Effects of Different Auxin–Cytokinin Combinations on Morphogenesis of Fritillaria meleagris Using Bulb Scale Sections In Vitro. in Horticulturae. 2023;9(8):910.
doi:10.3390/horticulturae9080910 .

Marković, Marija, Trifunović-Momčilov, Milana, Radulović, Olga, Paunović, Danijela, Antonić Reljin, Dragana, Uzelac, Branka, Subotić, Angelina, "The Effects of Different Auxin–Cytokinin Combinations on Morphogenesis of Fritillaria meleagris Using Bulb Scale Sections In Vitro" in Horticulturae, 9, no. 8 (2023):910,
https://doi.org/10.3390/horticulturae9080910 . .

TY  - CONF
AU  - Radulović, Olga
AU  - Popržen, Tatjana
AU  - Marković, Marija
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6193
AB  - Cilj ovog rada je bila analiza rizosfere sočivice kao mesta aktivne
produkcije auksina bakterijskog porekla, primarno indol-3-acetatne kiseline (IAA). Produkcija IAA je testirana na 21 različitom bakterijskom soju. Četiri bakterijska soja: Pseudomonas oryzihabitans 15, Pseudomonas putida 23, Hafnia paralvei 43 i Pseudomonas yamanorum 47 odabrana su na osnovu povećane produkcije IAA u odnosu na ostale testirane sojeve. Ovi sojevi su pokazali neprekinutu produkciju IAA i pod nepovoljnim uslovima: u kiseloj i baznoj sredini, kao i u hipersalinoj sredini. Naši rezultati sugerišu da ovi sojevi mogu imati biotehnološku primenu u zaštiti biljaka i povećanju prinosa putem produkcije auksina kao centralnih regulatora rasta i razvića biljaka.
AB  - The aim of this study was to analyze the rhizosphere of duckweed as a site of
active production of auxins of bacterial origin, primarily indole-3-acetic acid (IAA). IAA production was tested on 21 different bacterial strains. Four bacterial strains: Pseudomonas oryzihabitans 15, Pseudomonas putida 23, Hafnia paralvei 43 and Pseudomonas yamanorum 47 were selected based on increased IAA production compared to other strains tested. These strains showed continuous production of IAA even under unfavorable conditions: in acidic and basic environments, as well as in hypersaline environments. Our results suggest that these strains may have biotechnological applications in plant protection and increase yields through auxin production as central regulators of plant growth and development.
PB  - Kragujevac: Agronomsku fakultet u Čačku, Univerzitet u Kragujevcu
C3  - Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia
T1  - Potencijal rizosfere sočivice (Lemna minor L.) za produkciju auksina bakterijskog porekla
T1  - Potential of the rhizosphere of Duckweed (Lemna minor L.) for the production of auxins of bacterial origin
DO  - 10.46793/SBT27.401R
SP  - 401
EP  - 406
ER  - 

@conference{
author = "Radulović, Olga and Popržen, Tatjana and Marković, Marija",
year = "2022",
abstract = "Cilj ovog rada je bila analiza rizosfere sočivice kao mesta aktivne
produkcije auksina bakterijskog porekla, primarno indol-3-acetatne kiseline (IAA). Produkcija IAA je testirana na 21 različitom bakterijskom soju. Četiri bakterijska soja: Pseudomonas oryzihabitans 15, Pseudomonas putida 23, Hafnia paralvei 43 i Pseudomonas yamanorum 47 odabrana su na osnovu povećane produkcije IAA u odnosu na ostale testirane sojeve. Ovi sojevi su pokazali neprekinutu produkciju IAA i pod nepovoljnim uslovima: u kiseloj i baznoj sredini, kao i u hipersalinoj sredini. Naši rezultati sugerišu da ovi sojevi mogu imati biotehnološku primenu u zaštiti biljaka i povećanju prinosa putem produkcije auksina kao centralnih regulatora rasta i razvića biljaka., The aim of this study was to analyze the rhizosphere of duckweed as a site of
active production of auxins of bacterial origin, primarily indole-3-acetic acid (IAA). IAA production was tested on 21 different bacterial strains. Four bacterial strains: Pseudomonas oryzihabitans 15, Pseudomonas putida 23, Hafnia paralvei 43 and Pseudomonas yamanorum 47 were selected based on increased IAA production compared to other strains tested. These strains showed continuous production of IAA even under unfavorable conditions: in acidic and basic environments, as well as in hypersaline environments. Our results suggest that these strains may have biotechnological applications in plant protection and increase yields through auxin production as central regulators of plant growth and development.",
publisher = "Kragujevac: Agronomsku fakultet u Čačku, Univerzitet u Kragujevcu",
journal = "Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia",
title = "Potencijal rizosfere sočivice (Lemna minor L.) za produkciju auksina bakterijskog porekla, Potential of the rhizosphere of Duckweed (Lemna minor L.) for the production of auxins of bacterial origin",
doi = "10.46793/SBT27.401R",
pages = "401-406"
}

Radulović, O., Popržen, T.,& Marković, M.. (2022). Potencijal rizosfere sočivice (Lemna minor L.) za produkciju auksina bakterijskog porekla. in Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia
Kragujevac: Agronomsku fakultet u Čačku, Univerzitet u Kragujevcu., 401-406.
https://doi.org/10.46793/SBT27.401R

Radulović O, Popržen T, Marković M. Potencijal rizosfere sočivice (Lemna minor L.) za produkciju auksina bakterijskog porekla. in Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia. 2022;:401-406.
doi:10.46793/SBT27.401R .

Radulović, Olga, Popržen, Tatjana, Marković, Marija, "Potencijal rizosfere sočivice (Lemna minor L.) za produkciju auksina bakterijskog porekla" in Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia (2022):401-406,
https://doi.org/10.46793/SBT27.401R . .

TY  - CONF
AU  - Radulović, Olga
AU  - Popržen, Tatjana
AU  - Marković, Marija
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6195
AB  - Modern agriculture relies heavily on the use of synthetic herbicides: however, although efficient, these chemicals are also harmful to the environment. Therefore, there is an ever-increasing need for alternative herbicides: ethanol and indole-3-acetic acid (IAA) are among the candidates. Ethanol can be cheaply produced from a vast variety of substrates in large-scale fermentation processes. Moreover, in the last 2 decades, its applicability as an alternative, biosustainable herbicide is also being recognized. Ethanol has a well-known phytotoxic effect, although its mechanisms of actions are still poorly understood. IAA, a plant phytohormone, exerts its negative effect when applied in higher doses. Each plant has different sensitivity to ethanol and IAA. This property can be used to suppress the growth of harmful species (e.g. weeds) while stimulating the growth of desired cultures. In this study, we tested the effects of IAA and ethanol on duckweeds (Lemna minor L.). We measured non-enzymatic parameters of oxidative stress and multiplication rates of duckweeds exposed to IAA and to ethanol, respectively. Our results showed that higher concen-trations of IAA (>1 mg l-1) suppress the growth of duckweeds while even small amounts of ethanol (0.5% and 1% v/v) were sufficient to reduce the growth and biomass production of duckweeds. In conclusion, ethanol and IAA can exert significant negative effects on plant growth, at least in part by increasing oxidative stress in plants, and can therefore be potentially used as low-cost, eco-friendly herbicides.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Toward alternative herbicides: ethanol and indole-3-acetic acid in the suppression of plant growth
SP  - 100
EP  - 100
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6195
ER  - 

@conference{
author = "Radulović, Olga and Popržen, Tatjana and Marković, Marija",
year = "2022",
abstract = "Modern agriculture relies heavily on the use of synthetic herbicides: however, although efficient, these chemicals are also harmful to the environment. Therefore, there is an ever-increasing need for alternative herbicides: ethanol and indole-3-acetic acid (IAA) are among the candidates. Ethanol can be cheaply produced from a vast variety of substrates in large-scale fermentation processes. Moreover, in the last 2 decades, its applicability as an alternative, biosustainable herbicide is also being recognized. Ethanol has a well-known phytotoxic effect, although its mechanisms of actions are still poorly understood. IAA, a plant phytohormone, exerts its negative effect when applied in higher doses. Each plant has different sensitivity to ethanol and IAA. This property can be used to suppress the growth of harmful species (e.g. weeds) while stimulating the growth of desired cultures. In this study, we tested the effects of IAA and ethanol on duckweeds (Lemna minor L.). We measured non-enzymatic parameters of oxidative stress and multiplication rates of duckweeds exposed to IAA and to ethanol, respectively. Our results showed that higher concen-trations of IAA (>1 mg l-1) suppress the growth of duckweeds while even small amounts of ethanol (0.5% and 1% v/v) were sufficient to reduce the growth and biomass production of duckweeds. In conclusion, ethanol and IAA can exert significant negative effects on plant growth, at least in part by increasing oxidative stress in plants, and can therefore be potentially used as low-cost, eco-friendly herbicides.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Toward alternative herbicides: ethanol and indole-3-acetic acid in the suppression of plant growth",
pages = "100-100",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6195"
}

Radulović, O., Popržen, T.,& Marković, M.. (2022). Toward alternative herbicides: ethanol and indole-3-acetic acid in the suppression of plant growth. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 100-100.
https://hdl.handle.net/21.15107/rcub_ibiss_6195

Radulović O, Popržen T, Marković M. Toward alternative herbicides: ethanol and indole-3-acetic acid in the suppression of plant growth. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:100-100.
https://hdl.handle.net/21.15107/rcub_ibiss_6195 .

Radulović, Olga, Popržen, Tatjana, Marković, Marija, "Toward alternative herbicides: ethanol and indole-3-acetic acid in the suppression of plant growth" in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):100-100,
https://hdl.handle.net/21.15107/rcub_ibiss_6195 .

TY  - CONF
AU  - Radulović, Olga
AU  - Gašić, Uroš
AU  - Marković, Marija
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5666
AB  - The common duckweed (Lemna minor L.) as a model organism is experiencing a form of a renaissance. In this study, our focus was on the interactions between duckweeds and a rhizosphere-associated bacterial strain, Klebsiella oxytoca (Access. No. MK212915). Five distinct phenolic compounds were identified by liquid chromatography–mass spectrometry: luteolin 6,8-di-C-hexoside, p-hydroxybenzoic acid, caffeic acid, apigenin 6-C-(2′′-pentosyl)hexoside and p-coumaric acid. All of the identified compounds reflect the colonization of the plant by K. oxytoca. This paper is another call for all plant physiologists to focus their research on L. minor and to analyze different aspects of complex plant/bacterium interactions.
PB  - Basel: MDPI
C3  - The 2nd International Electronic Conference on Plant Sciences—10th Anniversary of Journal Plants; 2021 Dec 1-15; Online
T1  - Phenolic Compounds as Biomarkers of Interactions between the Endophyte Klebsiella oxytoca and the Common Duckweed, Lemna minor L.
DO  - 10.3390/IECPS2021-11924
SP  - 52
ER  - 

@conference{
author = "Radulović, Olga and Gašić, Uroš and Marković, Marija",
year = "2021",
abstract = "The common duckweed (Lemna minor L.) as a model organism is experiencing a form of a renaissance. In this study, our focus was on the interactions between duckweeds and a rhizosphere-associated bacterial strain, Klebsiella oxytoca (Access. No. MK212915). Five distinct phenolic compounds were identified by liquid chromatography–mass spectrometry: luteolin 6,8-di-C-hexoside, p-hydroxybenzoic acid, caffeic acid, apigenin 6-C-(2′′-pentosyl)hexoside and p-coumaric acid. All of the identified compounds reflect the colonization of the plant by K. oxytoca. This paper is another call for all plant physiologists to focus their research on L. minor and to analyze different aspects of complex plant/bacterium interactions.",
publisher = "Basel: MDPI",
journal = "The 2nd International Electronic Conference on Plant Sciences—10th Anniversary of Journal Plants; 2021 Dec 1-15; Online",
title = "Phenolic Compounds as Biomarkers of Interactions between the Endophyte Klebsiella oxytoca and the Common Duckweed, Lemna minor L.",
doi = "10.3390/IECPS2021-11924",
pages = "52"
}

Radulović, O., Gašić, U.,& Marković, M.. (2021). Phenolic Compounds as Biomarkers of Interactions between the Endophyte Klebsiella oxytoca and the Common Duckweed, Lemna minor L.. in The 2nd International Electronic Conference on Plant Sciences—10th Anniversary of Journal Plants; 2021 Dec 1-15; Online
Basel: MDPI., 52.
https://doi.org/10.3390/IECPS2021-11924

Radulović O, Gašić U, Marković M. Phenolic Compounds as Biomarkers of Interactions between the Endophyte Klebsiella oxytoca and the Common Duckweed, Lemna minor L.. in The 2nd International Electronic Conference on Plant Sciences—10th Anniversary of Journal Plants; 2021 Dec 1-15; Online. 2021;:52.
doi:10.3390/IECPS2021-11924 .

Radulović, Olga, Gašić, Uroš, Marković, Marija, "Phenolic Compounds as Biomarkers of Interactions between the Endophyte Klebsiella oxytoca and the Common Duckweed, Lemna minor L." in The 2nd International Electronic Conference on Plant Sciences—10th Anniversary of Journal Plants; 2021 Dec 1-15; Online (2021):52,
https://doi.org/10.3390/IECPS2021-11924 . .

TY  - JOUR
AU  - Radulović, Olga
AU  - Stanković, Slaviša
AU  - Stanojević, Olja
AU  - Vujčić, Zoran
AU  - Dojnov, Biljana
AU  - Trifunović-Momčilov, Milana
AU  - Marković, Marija
PY  - 2021
UR  - https://www.mdpi.com/2076-3921/10/11/1719
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4653
AB  - Duckweed (L. minor) is a cosmopolitan aquatic plant of simplified morphology and rapid vegetative reproduction. In this study, an H. paralvei bacterial strain and its influence on the antioxidative response of the duckweeds to phenol, a recalcitrant environmental pollutant, were investigated. Sterile duckweed cultures were inoculated with H. paralvei in vitro and cultivated in the presence or absence of phenol (500 mg L−1), in order to investigate bacterial effects on plant oxidative stress during 5 days. Total soluble proteins, guaiacol peroxidase expression, concentration of hydrogen peroxide and malondialdehyde as well as the total ascorbic acid of the plants were monitored. Moreover, bacterial production of indole-3-acetic acid (IAA) was measured in order to investigate H. paralvei’s influence on plant growth. In general, the addition of phenol elevated all biochemical parameters in L. minor except AsA and total soluble proteins. Phenol as well as bacteria influenced the expression of guaiacol peroxidase. Different isoforms were associated with phenol compared to isoforms expressed in phenol-free medium. Considering that duckweeds showed increased antioxidative parameters in the presence of phenol, it can be assumed that the measured parameters might be involved in the plant’s defense system. H. paralvei is an IAA producer and its presence in the rhizosphere of duckweeds decreased the oxidative stress of the plants, which can be taken as evidence that this bacterial strain acts protectively on the plants during phenol exposure.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Antioxidative Responses of Duckweed (Lemna minor L.) to Phenol and Rhizosphere-Associated Bacterial Strain Hafnia paralvei C32-106/3
IS  - 11
VL  - 10
DO  - 10.3390/antiox10111719
SP  - 1719
ER  - 

@article{
author = "Radulović, Olga and Stanković, Slaviša and Stanojević, Olja and Vujčić, Zoran and Dojnov, Biljana and Trifunović-Momčilov, Milana and Marković, Marija",
year = "2021",
abstract = "Duckweed (L. minor) is a cosmopolitan aquatic plant of simplified morphology and rapid vegetative reproduction. In this study, an H. paralvei bacterial strain and its influence on the antioxidative response of the duckweeds to phenol, a recalcitrant environmental pollutant, were investigated. Sterile duckweed cultures were inoculated with H. paralvei in vitro and cultivated in the presence or absence of phenol (500 mg L−1), in order to investigate bacterial effects on plant oxidative stress during 5 days. Total soluble proteins, guaiacol peroxidase expression, concentration of hydrogen peroxide and malondialdehyde as well as the total ascorbic acid of the plants were monitored. Moreover, bacterial production of indole-3-acetic acid (IAA) was measured in order to investigate H. paralvei’s influence on plant growth. In general, the addition of phenol elevated all biochemical parameters in L. minor except AsA and total soluble proteins. Phenol as well as bacteria influenced the expression of guaiacol peroxidase. Different isoforms were associated with phenol compared to isoforms expressed in phenol-free medium. Considering that duckweeds showed increased antioxidative parameters in the presence of phenol, it can be assumed that the measured parameters might be involved in the plant’s defense system. H. paralvei is an IAA producer and its presence in the rhizosphere of duckweeds decreased the oxidative stress of the plants, which can be taken as evidence that this bacterial strain acts protectively on the plants during phenol exposure.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Antioxidative Responses of Duckweed (Lemna minor L.) to Phenol and Rhizosphere-Associated Bacterial Strain Hafnia paralvei C32-106/3",
number = "11",
volume = "10",
doi = "10.3390/antiox10111719",
pages = "1719"
}

Radulović, O., Stanković, S., Stanojević, O., Vujčić, Z., Dojnov, B., Trifunović-Momčilov, M.,& Marković, M.. (2021). Antioxidative Responses of Duckweed (Lemna minor L.) to Phenol and Rhizosphere-Associated Bacterial Strain Hafnia paralvei C32-106/3. in Antioxidants
Basel: MDPI., 10(11), 1719.
https://doi.org/10.3390/antiox10111719

Radulović O, Stanković S, Stanojević O, Vujčić Z, Dojnov B, Trifunović-Momčilov M, Marković M. Antioxidative Responses of Duckweed (Lemna minor L.) to Phenol and Rhizosphere-Associated Bacterial Strain Hafnia paralvei C32-106/3. in Antioxidants. 2021;10(11):1719.
doi:10.3390/antiox10111719 .

Radulović, Olga, Stanković, Slaviša, Stanojević, Olja, Vujčić, Zoran, Dojnov, Biljana, Trifunović-Momčilov, Milana, Marković, Marija, "Antioxidative Responses of Duckweed (Lemna minor L.) to Phenol and Rhizosphere-Associated Bacterial Strain Hafnia paralvei C32-106/3" in Antioxidants, 10, no. 11 (2021):1719,
https://doi.org/10.3390/antiox10111719 . .

TY  - JOUR
AU  - Marković, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Uzelac, Branka
AU  - Radulović, Olga
AU  - Milošević, Snežana
AU  - Jevremović, Slađana
AU  - Subotić, Angelina
PY  - 2020
UR  - https://www.mdpi.com/2223-7747/9/11/1573
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4010
AB  - The bulb is the main propagation organ of snake’s head fritillary (Fritillaria meleagris L.), a horticulturally attractive and rare geophyte plant species. In this study, we investigated the effect of soaking bulbs in GA3 solution (1, 2, and 3 mg L−1) combined with low-temperature treatment (7 °C) on breaking the dormancy of in vitro bulbs. Sugar status (total soluble sugars, glucose, and fructose content) was analyzed in different parts of the sprouted bulbs. The results showed that the soluble sugar concentration was highest in bulbs soaked in GA3. The main sugar in fritillary bulbs was glucose, while fructose content was much lower. Glucose concentration dramatically increased after bulb chilling (7 °C), and its accumulation was predominantly detected in the lower sprout portion during the first weeks of sprouting. Sugar concentration was significantly lower in nonchilled bulbs, which indicates the importance of low temperature in bulb development and sprouting.
PB  - MDPI AG
T2  - Plants
T1  - Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 2: Effect of GA3 Soaking and Chilling on Sugar Status in Sprouted Bulbs
IS  - 11
VL  - 9
DO  - 10.3390/plants9111573
SP  - 1573
ER  - 

@article{
author = "Marković, Marija and Trifunović-Momčilov, Milana and Uzelac, Branka and Radulović, Olga and Milošević, Snežana and Jevremović, Slađana and Subotić, Angelina",
year = "2020",
abstract = "The bulb is the main propagation organ of snake’s head fritillary (Fritillaria meleagris L.), a horticulturally attractive and rare geophyte plant species. In this study, we investigated the effect of soaking bulbs in GA3 solution (1, 2, and 3 mg L−1) combined with low-temperature treatment (7 °C) on breaking the dormancy of in vitro bulbs. Sugar status (total soluble sugars, glucose, and fructose content) was analyzed in different parts of the sprouted bulbs. The results showed that the soluble sugar concentration was highest in bulbs soaked in GA3. The main sugar in fritillary bulbs was glucose, while fructose content was much lower. Glucose concentration dramatically increased after bulb chilling (7 °C), and its accumulation was predominantly detected in the lower sprout portion during the first weeks of sprouting. Sugar concentration was significantly lower in nonchilled bulbs, which indicates the importance of low temperature in bulb development and sprouting.",
publisher = "MDPI AG",
journal = "Plants",
title = "Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 2: Effect of GA3 Soaking and Chilling on Sugar Status in Sprouted Bulbs",
number = "11",
volume = "9",
doi = "10.3390/plants9111573",
pages = "1573"
}

Marković, M., Trifunović-Momčilov, M., Uzelac, B., Radulović, O., Milošević, S., Jevremović, S.,& Subotić, A.. (2020). Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 2: Effect of GA3 Soaking and Chilling on Sugar Status in Sprouted Bulbs. in Plants
MDPI AG., 9(11), 1573.
https://doi.org/10.3390/plants9111573

Marković M, Trifunović-Momčilov M, Uzelac B, Radulović O, Milošević S, Jevremović S, Subotić A. Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 2: Effect of GA3 Soaking and Chilling on Sugar Status in Sprouted Bulbs. in Plants. 2020;9(11):1573.
doi:10.3390/plants9111573 .

Marković, Marija, Trifunović-Momčilov, Milana, Uzelac, Branka, Radulović, Olga, Milošević, Snežana, Jevremović, Slađana, Subotić, Angelina, "Breaking the Dormancy of Snake’s Head Fritillary (Fritillaria meleagris L.) In Vitro Bulbs—Part 2: Effect of GA3 Soaking and Chilling on Sugar Status in Sprouted Bulbs" in Plants, 9, no. 11 (2020):1573,
https://doi.org/10.3390/plants9111573 . .

TY  - THES
AU  - Radulović, Olga
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4050
AB  - U ovoj doktorskoj disertaciji ispitivano je uklanjanje fenola iz tečne hranljive podloge pomoću sočivice (Lemna minor L.) i bakterija njene rizosfere. Od ukupnog broja bakterijskih izolata rizosfere, izolovano je 6 sojeva kao najbolji kandidati za uklanjanje fenola: Lelliottia sp. 11, Klebsiella oxytoca 14, Serratia marcescens 27, Hafnia alvei 37, H. paralvei 43, i S. nematodiphila 51. Sočivice inokulisane bilo kojim od ovih sojeva efikasnije u uklanjanju fenol od površinski sterilisanih sočivica kao i od bilo kog od ovih sojeva u obliku slobodne suspenzije (bez sočivica). Soj H. paralvei 43, u kombinaciji sa sočivicom, bio je najefikasniji u uklanjanju fenola. Ovaj bakterijski soj utiče i na antioksidativni odgovor sočivice što se ogleda u povećanim enzimskim (GPX) i neenzimskim (ukupni solubilni proteini biljnog tkiva, MDA i H2O2) parametrima oksidativnog stresa.Zimogramskom detekcijom GPX utvrđeno je prisustvo pet različitih izoformi u tkivu sočivice gajenoj u prisustvu fenola, dok su četiri izoforme detektovane kada su na korenu bile prisutne bakterije bez fenola Fluorescentom mikroskopijom pokazano je da je K. oxytoca 14 endofit sa najizraženijim efektom na multiplikaciju sočivica sa ili bez fenola. Iz hranljive podloge bez fenola u kojoj su gajene sočivice i K. oxytoca 14 izolovano je 6 fenolnih jedinjenja: luteolin 6,8-di-C-heksozid, p-hidroksibenzoeva kiselina, kafeinska kiselina, apigenin 6-C-(2"-pentozil)heksozid i p-kumarinska kiselina. Rezultati ove doktorske disertacije pokazuju da su odabrani bakterijski sojevi u kombinaciji sa biljkama sočivice potencijalno rešenje za bioremedijaciju vode zagađene fenolom.
AB  - In this dissertation, the possibility of phenol removal by the common duckweed (Lemna minor L.) and by the bacteria of its rhizosphere, was investigated. Six bacterial strains, selected according to their ability of growth on the minimal nutrient medium with phenol, high phenol-resistance, sensitivity to typical antibiotics, biofilm formation, positive effect on the multiplication rates of the plants and bacteria-plant interactions on the root surface were: Lelliottia sp. 11, Klebsiella oxytoca 14, Serratia marcescens 27, Hafnia alvei 37, H. paralvei 43, and S. nematodiphila 51. Duckweed inoculated with any of the aforementioned strains were more efficient in phenol removal than the surface-sterilized duckweed or the bacterial suspensions. The most efficient bacterial strain/duckweed system is the one based on H. paralvei 43 and plants. This bacterial strain also modulates the antioxidant response of the plant, indicated by the non-enzymatic (total AsA, soluble proteins, MDA and H2O2) and the enzymatic parameters. The zymogram detection of GPX in the plants showed 5 phenol-induced GPX isoforms, whereas the bacteria alone induced 4 different GPX isoforms. Bacteria showed a tendency to lower the intensity of GPX expression in plants. K. oxytoca 14 is an endophyte which induced a rapid multiplication of the duckweed. Six phenolic compounds were identified in the nutrient medium used to co-culture K. oxytoca 14 and the duckweed: lutheolin 6,8-di-C-hexoside, p-hidroxybenzoic acid, caffeic acid, apigenin 6-C-(2"-pentosyl) hexoside and p-coumaric acid. The results of this dissertation show that the selected bacteria /duckweed systems present an efficient solution for the bioremediation of the phenol-polluted wastewaters.
PB  - Belgrade: University of Belgrade, Faculty of Biology
T2  - University of Belgrade, Faculty of Biology
T1  - Sistem za bioremedijaciju voda zagađenih fenolom upotrebom sočivice (Lemna minor L.) i bakterija njene rizosfere
T1  - System for bioremediation of phenol-polluted waters based on the application of the common duckweed (Lemna minor L.) and the bacteria of its rhizosphere
SP  - 1
EP  - 88
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4050
ER  - 

@phdthesis{
author = "Radulović, Olga",
year = "2020",
abstract = "U ovoj doktorskoj disertaciji ispitivano je uklanjanje fenola iz tečne hranljive podloge pomoću sočivice (Lemna minor L.) i bakterija njene rizosfere. Od ukupnog broja bakterijskih izolata rizosfere, izolovano je 6 sojeva kao najbolji kandidati za uklanjanje fenola: Lelliottia sp. 11, Klebsiella oxytoca 14, Serratia marcescens 27, Hafnia alvei 37, H. paralvei 43, i S. nematodiphila 51. Sočivice inokulisane bilo kojim od ovih sojeva efikasnije u uklanjanju fenol od površinski sterilisanih sočivica kao i od bilo kog od ovih sojeva u obliku slobodne suspenzije (bez sočivica). Soj H. paralvei 43, u kombinaciji sa sočivicom, bio je najefikasniji u uklanjanju fenola. Ovaj bakterijski soj utiče i na antioksidativni odgovor sočivice što se ogleda u povećanim enzimskim (GPX) i neenzimskim (ukupni solubilni proteini biljnog tkiva, MDA i H2O2) parametrima oksidativnog stresa.Zimogramskom detekcijom GPX utvrđeno je prisustvo pet različitih izoformi u tkivu sočivice gajenoj u prisustvu fenola, dok su četiri izoforme detektovane kada su na korenu bile prisutne bakterije bez fenola Fluorescentom mikroskopijom pokazano je da je K. oxytoca 14 endofit sa najizraženijim efektom na multiplikaciju sočivica sa ili bez fenola. Iz hranljive podloge bez fenola u kojoj su gajene sočivice i K. oxytoca 14 izolovano je 6 fenolnih jedinjenja: luteolin 6,8-di-C-heksozid, p-hidroksibenzoeva kiselina, kafeinska kiselina, apigenin 6-C-(2"-pentozil)heksozid i p-kumarinska kiselina. Rezultati ove doktorske disertacije pokazuju da su odabrani bakterijski sojevi u kombinaciji sa biljkama sočivice potencijalno rešenje za bioremedijaciju vode zagađene fenolom., In this dissertation, the possibility of phenol removal by the common duckweed (Lemna minor L.) and by the bacteria of its rhizosphere, was investigated. Six bacterial strains, selected according to their ability of growth on the minimal nutrient medium with phenol, high phenol-resistance, sensitivity to typical antibiotics, biofilm formation, positive effect on the multiplication rates of the plants and bacteria-plant interactions on the root surface were: Lelliottia sp. 11, Klebsiella oxytoca 14, Serratia marcescens 27, Hafnia alvei 37, H. paralvei 43, and S. nematodiphila 51. Duckweed inoculated with any of the aforementioned strains were more efficient in phenol removal than the surface-sterilized duckweed or the bacterial suspensions. The most efficient bacterial strain/duckweed system is the one based on H. paralvei 43 and plants. This bacterial strain also modulates the antioxidant response of the plant, indicated by the non-enzymatic (total AsA, soluble proteins, MDA and H2O2) and the enzymatic parameters. The zymogram detection of GPX in the plants showed 5 phenol-induced GPX isoforms, whereas the bacteria alone induced 4 different GPX isoforms. Bacteria showed a tendency to lower the intensity of GPX expression in plants. K. oxytoca 14 is an endophyte which induced a rapid multiplication of the duckweed. Six phenolic compounds were identified in the nutrient medium used to co-culture K. oxytoca 14 and the duckweed: lutheolin 6,8-di-C-hexoside, p-hidroxybenzoic acid, caffeic acid, apigenin 6-C-(2"-pentosyl) hexoside and p-coumaric acid. The results of this dissertation show that the selected bacteria /duckweed systems present an efficient solution for the bioremediation of the phenol-polluted wastewaters.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "University of Belgrade, Faculty of Biology",
title = "Sistem za bioremedijaciju voda zagađenih fenolom upotrebom sočivice (Lemna minor L.) i bakterija njene rizosfere, System for bioremediation of phenol-polluted waters based on the application of the common duckweed (Lemna minor L.) and the bacteria of its rhizosphere",
pages = "1-88",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4050"
}

Radulović, O.. (2020). Sistem za bioremedijaciju voda zagađenih fenolom upotrebom sočivice (Lemna minor L.) i bakterija njene rizosfere. in University of Belgrade, Faculty of Biology
Belgrade: University of Belgrade, Faculty of Biology., 1-88.
https://hdl.handle.net/21.15107/rcub_ibiss_4050

Radulović O. Sistem za bioremedijaciju voda zagađenih fenolom upotrebom sočivice (Lemna minor L.) i bakterija njene rizosfere. in University of Belgrade, Faculty of Biology. 2020;:1-88.
https://hdl.handle.net/21.15107/rcub_ibiss_4050 .

Radulović, Olga, "Sistem za bioremedijaciju voda zagađenih fenolom upotrebom sočivice (Lemna minor L.) i bakterija njene rizosfere" in University of Belgrade, Faculty of Biology (2020):1-88,
https://hdl.handle.net/21.15107/rcub_ibiss_4050 .

TY  - JOUR
AU  - Radulović, Olga
AU  - Stanković, Slaviša
AU  - Uzelac, Branka
AU  - Tadić, Vojin
AU  - Trifunović-Momčilov, Milana
AU  - Lozo, Jelena
AU  - Marković, Marija
PY  - 2020
UR  - https://www.mdpi.com/2223-7747/9/5/599
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3676
AB  - The main topic of this study is the bioremediation potential of the common duckweed, Lemna minor L., and selected rhizospheric bacterial strains in removing phenol from aqueous environments at extremely high initial phenol concentrations. To that end, fluorescence microscopy, MIC tests, biofilm formation, the phenol removal test (4-AAP method), the Salkowski essay, and studies of multiplication rates of sterile and inoculated duckweed in MS medium with phenol (200, 500, 750, and 1000 mg L−1) were conducted. Out of seven bacterial strains, six were identified as epiphytes or endophytes that efficiently removed phenol. The phenol removal experiment showed that the bacteria/duckweed system was more efficient during the first 24 h compared to the sterile duckweed control group. At the end of this experiment, almost 90% of the initial phenol concentration was removed by both groups, respectively. The bacteria stimulated the duckweed multiplication even at a high bacterial population density (>105 CFU mL−1) over a prolonged period of time (14 days). All bacterial strains were sensitive to all the applied antibiotics and formed biofilms in vitro. The dual bacteria/duckweed system, especially the one containing strain 43-Hafnia paralvei C32-106/3, Accession No. MF526939, had a number of characteristics that are advantageous in bioremediation, such as high phenol removal efficiency, biofilm formation, safety (antibiotic sensitivity), and stimulation of duckweed multiplication.
PB  - MDPI AG
T2  - Plants
T1  - Phenol Removal Capacity of the Common Duckweed (Lemna minor L.) and Six Phenol-Resistant Bacterial Strains From Its Rhizosphere: In Vitro Evaluation at High Phenol Concentrations
IS  - 5
VL  - 9
DO  - 10.3390/plants9050599
SP  - 599
ER  - 

@article{
author = "Radulović, Olga and Stanković, Slaviša and Uzelac, Branka and Tadić, Vojin and Trifunović-Momčilov, Milana and Lozo, Jelena and Marković, Marija",
year = "2020",
abstract = "The main topic of this study is the bioremediation potential of the common duckweed, Lemna minor L., and selected rhizospheric bacterial strains in removing phenol from aqueous environments at extremely high initial phenol concentrations. To that end, fluorescence microscopy, MIC tests, biofilm formation, the phenol removal test (4-AAP method), the Salkowski essay, and studies of multiplication rates of sterile and inoculated duckweed in MS medium with phenol (200, 500, 750, and 1000 mg L−1) were conducted. Out of seven bacterial strains, six were identified as epiphytes or endophytes that efficiently removed phenol. The phenol removal experiment showed that the bacteria/duckweed system was more efficient during the first 24 h compared to the sterile duckweed control group. At the end of this experiment, almost 90% of the initial phenol concentration was removed by both groups, respectively. The bacteria stimulated the duckweed multiplication even at a high bacterial population density (>105 CFU mL−1) over a prolonged period of time (14 days). All bacterial strains were sensitive to all the applied antibiotics and formed biofilms in vitro. The dual bacteria/duckweed system, especially the one containing strain 43-Hafnia paralvei C32-106/3, Accession No. MF526939, had a number of characteristics that are advantageous in bioremediation, such as high phenol removal efficiency, biofilm formation, safety (antibiotic sensitivity), and stimulation of duckweed multiplication.",
publisher = "MDPI AG",
journal = "Plants",
title = "Phenol Removal Capacity of the Common Duckweed (Lemna minor L.) and Six Phenol-Resistant Bacterial Strains From Its Rhizosphere: In Vitro Evaluation at High Phenol Concentrations",
number = "5",
volume = "9",
doi = "10.3390/plants9050599",
pages = "599"
}

Radulović, O., Stanković, S., Uzelac, B., Tadić, V., Trifunović-Momčilov, M., Lozo, J.,& Marković, M.. (2020). Phenol Removal Capacity of the Common Duckweed (Lemna minor L.) and Six Phenol-Resistant Bacterial Strains From Its Rhizosphere: In Vitro Evaluation at High Phenol Concentrations. in Plants
MDPI AG., 9(5), 599.
https://doi.org/10.3390/plants9050599

Radulović O, Stanković S, Uzelac B, Tadić V, Trifunović-Momčilov M, Lozo J, Marković M. Phenol Removal Capacity of the Common Duckweed (Lemna minor L.) and Six Phenol-Resistant Bacterial Strains From Its Rhizosphere: In Vitro Evaluation at High Phenol Concentrations. in Plants. 2020;9(5):599.
doi:10.3390/plants9050599 .

Radulović, Olga, Stanković, Slaviša, Uzelac, Branka, Tadić, Vojin, Trifunović-Momčilov, Milana, Lozo, Jelena, Marković, Marija, "Phenol Removal Capacity of the Common Duckweed (Lemna minor L.) and Six Phenol-Resistant Bacterial Strains From Its Rhizosphere: In Vitro Evaluation at High Phenol Concentrations" in Plants, 9, no. 5 (2020):599,
https://doi.org/10.3390/plants9050599 . .

TY  - JOUR
AU  - Radulović, Olga
AU  - Petrić, Marija
AU  - Raspor, Martin
AU  - Stanojević, Olja
AU  - Janakiev, Tamara
AU  - Tadić, Vojin
AU  - Stanković, Slaviša
PY  - 2019
UR  - http://www.pjoes.com/doi/10.15244/pjoes/81687
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3255
AB  - In this work, we demonstrate that the rhizosphere of common duckweed (Lemna minor) is inhabited with various phenol-resistant bacterial strains. Based on 16S rRNA sequencing, we have identified 60 rhizosphere-associated bacterial isolates belonging to 10 different bacterial genera (Pseudomonas, Hafnia, Serratia, Enterobacter, Micrococcus, Stenotrophomonas, Xanthomonas, Bacillus, Staphylococcus and Klebsiella). All isolates have been tested for phenol resistance and ability to utilize phenol as the sole carbon source. 70% of all isolates survived high doses of phenol (≥200 mg/L) and at least 27% can be potentially acclimatized by gradual increase of phenol concentration. Finally, based on high phenol resistance, ability to utilize phenol as the sole carbon source and documented low pathogenicity, we propose 5 strains as potentially excellent candidates for bioremediation. These 5 strains taxonomically correspond to Klebsiella sp., Serratia sp., and Hafnia sp., respectively. To the best of our knowledge, this is the first attempt to assess decontamination capacity of Serratia nematodiphila and Hafnia sp. in the context of bioremediation of phenol-contaminated aqueous media. Although additional analyses are needed, interaction between the common duckweed and the selected bacterial strains may be utilized in future bioremediation strategies.
T2  - Polish Journal of Environmental Studies
T1  - Culture-Dependent Analysis of 16S rRNA Sequences Associated with the Rhizosphere of Lemna minor and Assessment of Bacterial Phenol-Resistance: Plant/Bacteria System for Potential Bioremediation – Part II
IS  - 2
VL  - 28
DO  - 10.15244/pjoes/81687
SP  - 811
EP  - 822
ER  - 

@article{
author = "Radulović, Olga and Petrić, Marija and Raspor, Martin and Stanojević, Olja and Janakiev, Tamara and Tadić, Vojin and Stanković, Slaviša",
year = "2019",
abstract = "In this work, we demonstrate that the rhizosphere of common duckweed (Lemna minor) is inhabited with various phenol-resistant bacterial strains. Based on 16S rRNA sequencing, we have identified 60 rhizosphere-associated bacterial isolates belonging to 10 different bacterial genera (Pseudomonas, Hafnia, Serratia, Enterobacter, Micrococcus, Stenotrophomonas, Xanthomonas, Bacillus, Staphylococcus and Klebsiella). All isolates have been tested for phenol resistance and ability to utilize phenol as the sole carbon source. 70% of all isolates survived high doses of phenol (≥200 mg/L) and at least 27% can be potentially acclimatized by gradual increase of phenol concentration. Finally, based on high phenol resistance, ability to utilize phenol as the sole carbon source and documented low pathogenicity, we propose 5 strains as potentially excellent candidates for bioremediation. These 5 strains taxonomically correspond to Klebsiella sp., Serratia sp., and Hafnia sp., respectively. To the best of our knowledge, this is the first attempt to assess decontamination capacity of Serratia nematodiphila and Hafnia sp. in the context of bioremediation of phenol-contaminated aqueous media. Although additional analyses are needed, interaction between the common duckweed and the selected bacterial strains may be utilized in future bioremediation strategies.",
journal = "Polish Journal of Environmental Studies",
title = "Culture-Dependent Analysis of 16S rRNA Sequences Associated with the Rhizosphere of Lemna minor and Assessment of Bacterial Phenol-Resistance: Plant/Bacteria System for Potential Bioremediation – Part II",
number = "2",
volume = "28",
doi = "10.15244/pjoes/81687",
pages = "811-822"
}

Radulović, O., Petrić, M., Raspor, M., Stanojević, O., Janakiev, T., Tadić, V.,& Stanković, S.. (2019). Culture-Dependent Analysis of 16S rRNA Sequences Associated with the Rhizosphere of Lemna minor and Assessment of Bacterial Phenol-Resistance: Plant/Bacteria System for Potential Bioremediation – Part II. in Polish Journal of Environmental Studies, 28(2), 811-822.
https://doi.org/10.15244/pjoes/81687

Radulović O, Petrić M, Raspor M, Stanojević O, Janakiev T, Tadić V, Stanković S. Culture-Dependent Analysis of 16S rRNA Sequences Associated with the Rhizosphere of Lemna minor and Assessment of Bacterial Phenol-Resistance: Plant/Bacteria System for Potential Bioremediation – Part II. in Polish Journal of Environmental Studies. 2019;28(2):811-822.
doi:10.15244/pjoes/81687 .

Radulović, Olga, Petrić, Marija, Raspor, Martin, Stanojević, Olja, Janakiev, Tamara, Tadić, Vojin, Stanković, Slaviša, "Culture-Dependent Analysis of 16S rRNA Sequences Associated with the Rhizosphere of Lemna minor and Assessment of Bacterial Phenol-Resistance: Plant/Bacteria System for Potential Bioremediation – Part II" in Polish Journal of Environmental Studies, 28, no. 2 (2019):811-822,
https://doi.org/10.15244/pjoes/81687 . .

TY  - JOUR
AU  - Radulović, Olga
AU  - Petrić, Marija
AU  - Raspor, Martin
AU  - Tadić, Vojin
AU  - Jovanović, Petar
AU  - Zečević, Vladan
PY  - 2019
UR  - http://www.journalssystem.com/pjoes/Assessment-of-in-vitro-multiplication-of-Lemna-minor-in-the-presence-of-phenol-plant,84921,0,2.html
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3251
AB  - The aim of this work was to examine the multiplication of the common duckweed (Lemna minor), an aquatic plant species widespread in European stagnant waters, in two different media (Murashige – Skoog and Hoagland) with and without phenol supplementation. In order to quantify plant multiplication we have used relative growth rate and tolerance indices on both tested media and at five phenol concentrations (10, 15, 20, 30 and 100 mg/L). Furthermore, we examined the possibility of phenol removal from aqueous media containing different phenol concentrations, by using plant/bacteria system consisting of the duckweed and its naturally occurring microbial populations. After 7 days, number of newly formed fronds was approximately four times higher than at the beginning of the experiment on both tested media. The most important result in this study was removal of 70% of phenol from the highest initial concentration of 100 mg/L, in mixed cultures of duckweed and bacteria. By comparison, aseptic duckweed cultures removed approximately 50% of phenol at the same initial concentration. Our duckweed specimen showed a fast reproduction rate, high tolerance to phenol and a possible cooperation with rhizosphere-associated bacteria. All of these traits can be ultimately utilized for bioremediation purposes.
T2  - Polish Journal of Environmental Studies
T1  - Assessment of in vitro Multiplication of Lemna minor in the Presence of Phenol: Plant/Bacteria System for Potential Bioremediation – Part I
IS  - 2
VL  - 28
DO  - 10.15244/pjoes/84921
SP  - 803
EP  - 809
ER  - 

@article{
author = "Radulović, Olga and Petrić, Marija and Raspor, Martin and Tadić, Vojin and Jovanović, Petar and Zečević, Vladan",
year = "2019",
abstract = "The aim of this work was to examine the multiplication of the common duckweed (Lemna minor), an aquatic plant species widespread in European stagnant waters, in two different media (Murashige – Skoog and Hoagland) with and without phenol supplementation. In order to quantify plant multiplication we have used relative growth rate and tolerance indices on both tested media and at five phenol concentrations (10, 15, 20, 30 and 100 mg/L). Furthermore, we examined the possibility of phenol removal from aqueous media containing different phenol concentrations, by using plant/bacteria system consisting of the duckweed and its naturally occurring microbial populations. After 7 days, number of newly formed fronds was approximately four times higher than at the beginning of the experiment on both tested media. The most important result in this study was removal of 70% of phenol from the highest initial concentration of 100 mg/L, in mixed cultures of duckweed and bacteria. By comparison, aseptic duckweed cultures removed approximately 50% of phenol at the same initial concentration. Our duckweed specimen showed a fast reproduction rate, high tolerance to phenol and a possible cooperation with rhizosphere-associated bacteria. All of these traits can be ultimately utilized for bioremediation purposes.",
journal = "Polish Journal of Environmental Studies",
title = "Assessment of in vitro Multiplication of Lemna minor in the Presence of Phenol: Plant/Bacteria System for Potential Bioremediation – Part I",
number = "2",
volume = "28",
doi = "10.15244/pjoes/84921",
pages = "803-809"
}

Radulović, O., Petrić, M., Raspor, M., Tadić, V., Jovanović, P.,& Zečević, V.. (2019). Assessment of in vitro Multiplication of Lemna minor in the Presence of Phenol: Plant/Bacteria System for Potential Bioremediation – Part I. in Polish Journal of Environmental Studies, 28(2), 803-809.
https://doi.org/10.15244/pjoes/84921

Radulović O, Petrić M, Raspor M, Tadić V, Jovanović P, Zečević V. Assessment of in vitro Multiplication of Lemna minor in the Presence of Phenol: Plant/Bacteria System for Potential Bioremediation – Part I. in Polish Journal of Environmental Studies. 2019;28(2):803-809.
doi:10.15244/pjoes/84921 .

Radulović, Olga, Petrić, Marija, Raspor, Martin, Tadić, Vojin, Jovanović, Petar, Zečević, Vladan, "Assessment of in vitro Multiplication of Lemna minor in the Presence of Phenol: Plant/Bacteria System for Potential Bioremediation – Part I" in Polish Journal of Environmental Studies, 28, no. 2 (2019):803-809,
https://doi.org/10.15244/pjoes/84921 . .

TY  - JOUR
AU  - Milošević, Zorica C.
AU  - Nadrljanski, Mirjan M.
AU  - Milovanović, Zorka M.
AU  - Gušić, Nina Z.
AU  - Vučićević, Slavko S.
AU  - Radulović, Olga
PY  - 2017
UR  - http://www.degruyter.com/view/j/raon.2017.51.issue-2/raon-2017-0016/raon-2017-0016.xml
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2781
AB  - We aimed to analyse the morphokinetic features of breast fibrocystic changes (nonproliferative lesions, proliferative lesions without atypia and proliferative lesions with atypia) presenting as a non-mass enhancement (NME)in dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) examination. Forty-six patients with histologically proven fibrocystic changes (FCCs) were retrospectively reviewed, according to Breast Imaging Reporting and Data System (BI-RADS) lexicon. Prior to DCE-MRI examination, a unilateral breast lesion suspicious of malignancy was detected clinically, on mammography or breast ultrasonography. The predominant features of FCCs presenting as NME in DCE-MRI examination were: unilateral regional or diffuse distribution (in 35 patients or 76.1%), heterogeneous or clumped internal pattern of enhancement (in 36 patients or 78.3%), plateau time-intensity curve (in 25 patients or 54.3%), moderate or fast wash-in (in 31 patients or 67.4%).Nonproliferative lesions were found in 11 patients (24%), proliferative lesions without atypia in 29 patients (63%) and lesions with atypia in six patients (13%), without statistically significant difference of morphokinetic features, except of the association of clustered microcysts with proliferative dysplasia without atypia. FCCs presenting as NME in DCE-MRI examination have several morphokinetic features suspicious of malignancy, therefore requiring biopsy (BI-RADS 4). Nonproliferative lesions, proliferative lesions without atypia and proliferative lesions with atypia predominantly share the same predefined DCE-MRI morphokinetic features.
T2  - Radiology and Oncology
T1  - Breast dynamic contrast enhanced MRI: fibrocystic changes presenting as a non-mass enhancement mimicking malignancy
IS  - 2
VL  - 51
DO  - 10.1515/raon-2017-0016
SP  - 130
EP  - 136
ER  - 

@article{
author = "Milošević, Zorica C. and Nadrljanski, Mirjan M. and Milovanović, Zorka M. and Gušić, Nina Z. and Vučićević, Slavko S. and Radulović, Olga",
year = "2017",
abstract = "We aimed to analyse the morphokinetic features of breast fibrocystic changes (nonproliferative lesions, proliferative lesions without atypia and proliferative lesions with atypia) presenting as a non-mass enhancement (NME)in dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) examination. Forty-six patients with histologically proven fibrocystic changes (FCCs) were retrospectively reviewed, according to Breast Imaging Reporting and Data System (BI-RADS) lexicon. Prior to DCE-MRI examination, a unilateral breast lesion suspicious of malignancy was detected clinically, on mammography or breast ultrasonography. The predominant features of FCCs presenting as NME in DCE-MRI examination were: unilateral regional or diffuse distribution (in 35 patients or 76.1%), heterogeneous or clumped internal pattern of enhancement (in 36 patients or 78.3%), plateau time-intensity curve (in 25 patients or 54.3%), moderate or fast wash-in (in 31 patients or 67.4%).Nonproliferative lesions were found in 11 patients (24%), proliferative lesions without atypia in 29 patients (63%) and lesions with atypia in six patients (13%), without statistically significant difference of morphokinetic features, except of the association of clustered microcysts with proliferative dysplasia without atypia. FCCs presenting as NME in DCE-MRI examination have several morphokinetic features suspicious of malignancy, therefore requiring biopsy (BI-RADS 4). Nonproliferative lesions, proliferative lesions without atypia and proliferative lesions with atypia predominantly share the same predefined DCE-MRI morphokinetic features.",
journal = "Radiology and Oncology",
title = "Breast dynamic contrast enhanced MRI: fibrocystic changes presenting as a non-mass enhancement mimicking malignancy",
number = "2",
volume = "51",
doi = "10.1515/raon-2017-0016",
pages = "130-136"
}

Milošević, Z. C., Nadrljanski, M. M., Milovanović, Z. M., Gušić, N. Z., Vučićević, S. S.,& Radulović, O.. (2017). Breast dynamic contrast enhanced MRI: fibrocystic changes presenting as a non-mass enhancement mimicking malignancy. in Radiology and Oncology, 51(2), 130-136.
https://doi.org/10.1515/raon-2017-0016

Milošević ZC, Nadrljanski MM, Milovanović ZM, Gušić NZ, Vučićević SS, Radulović O. Breast dynamic contrast enhanced MRI: fibrocystic changes presenting as a non-mass enhancement mimicking malignancy. in Radiology and Oncology. 2017;51(2):130-136.
doi:10.1515/raon-2017-0016 .

Milošević, Zorica C., Nadrljanski, Mirjan M., Milovanović, Zorka M., Gušić, Nina Z., Vučićević, Slavko S., Radulović, Olga, "Breast dynamic contrast enhanced MRI: fibrocystic changes presenting as a non-mass enhancement mimicking malignancy" in Radiology and Oncology, 51, no. 2 (2017):130-136,
https://doi.org/10.1515/raon-2017-0016 . .