TY  - CONF
AU  - Novaković, Andrea
AU  - Radovanović, Ljiljana
AU  - Petrović, Jelena
AU  - Šaponjić, Jasna
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5841
AB  - We examined the effects of ketamine/diazepam and propofol anesthesia on
hippocampal and reticulo-thalamic (RT) GABAergic parvalbumin (PV)-expressing
interneurons in the rat.
A total of 20 adult male Wistar rats were divided into two experimental groups - half
were anesthetized with ketamine/diazepam (100 mg/kg, Zoletil® 50), and the other
half received propofol anesthesia (100 mg/kg; Propofol Lipuro 2% (20mg/ml). We
performed immunohistochemistry protocols for PV and postsynaptic density protein
95 (PSD-95) staining on free-floating 40-µm brain slices. We used PSD-95 as an
excitatory synaptic marker to test local excitation changes along with changes in PV
expression.
Our results show significant suppression of GABAergic PV-expressing interneurons
during ketamine/diazepam anesthesia compared with propofol anesthesia, in the
dentate gyrus and CA3 regions of the hippocampus (z ≥ -4.16, p ≤ 10-3), and in the
RT. Moreover, this suppression resulted in an increase in PSD-95 expression only in
the hippocampus of rats anesthetized with ketamine/diazepam. Topographically
distinct effects of propofol anesthesia were not detected.
The observed imbalance between excitation and inhibition at the level of the
hippocampus during ketamine/diazepam anesthesia could be a consequence of lower
interneuronal GABA activity. Conversely, the topographically uniform expression of
PSD-95 during propofol anesthesia together with higher expression of GABAergic
interneurons could possibly indicate a stronger effect of GABA-mediated inhibition in
the hippocampus compared with ketamine/diazepam anesthesia.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - Effects of different anesthetics on hippocampal and reticulothalamic GABAergic parvalbumin-expressing interneurons
IS  - 52
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5841
ER  - 

@conference{
author = "Novaković, Andrea and Radovanović, Ljiljana and Petrović, Jelena and Šaponjić, Jasna",
year = "2023",
abstract = "We examined the effects of ketamine/diazepam and propofol anesthesia on
hippocampal and reticulo-thalamic (RT) GABAergic parvalbumin (PV)-expressing
interneurons in the rat.
A total of 20 adult male Wistar rats were divided into two experimental groups - half
were anesthetized with ketamine/diazepam (100 mg/kg, Zoletil® 50), and the other
half received propofol anesthesia (100 mg/kg; Propofol Lipuro 2% (20mg/ml). We
performed immunohistochemistry protocols for PV and postsynaptic density protein
95 (PSD-95) staining on free-floating 40-µm brain slices. We used PSD-95 as an
excitatory synaptic marker to test local excitation changes along with changes in PV
expression.
Our results show significant suppression of GABAergic PV-expressing interneurons
during ketamine/diazepam anesthesia compared with propofol anesthesia, in the
dentate gyrus and CA3 regions of the hippocampus (z ≥ -4.16, p ≤ 10-3), and in the
RT. Moreover, this suppression resulted in an increase in PSD-95 expression only in
the hippocampus of rats anesthetized with ketamine/diazepam. Topographically
distinct effects of propofol anesthesia were not detected.
The observed imbalance between excitation and inhibition at the level of the
hippocampus during ketamine/diazepam anesthesia could be a consequence of lower
interneuronal GABA activity. Conversely, the topographically uniform expression of
PSD-95 during propofol anesthesia together with higher expression of GABAergic
interneurons could possibly indicate a stronger effect of GABA-mediated inhibition in
the hippocampus compared with ketamine/diazepam anesthesia.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "Effects of different anesthetics on hippocampal and reticulothalamic GABAergic parvalbumin-expressing interneurons",
number = "52",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5841"
}

Novaković, A., Radovanović, L., Petrović, J.,& Šaponjić, J.. (2023). Effects of different anesthetics on hippocampal and reticulothalamic GABAergic parvalbumin-expressing interneurons. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society.(52).
https://hdl.handle.net/21.15107/rcub_ibiss_5841

Novaković A, Radovanović L, Petrović J, Šaponjić J. Effects of different anesthetics on hippocampal and reticulothalamic GABAergic parvalbumin-expressing interneurons. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;(52).
https://hdl.handle.net/21.15107/rcub_ibiss_5841 .

Novaković, Andrea, Radovanović, Ljiljana, Petrović, Jelena, Šaponjić, Jasna, "Effects of different anesthetics on hippocampal and reticulothalamic GABAergic parvalbumin-expressing interneurons" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia, no. 52 (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_5841 .

TY  - CONF
AU  - Šaponjić, Jasna
AU  - Radovanović, Ljiljana
AU  - Novaković, Andrea
AU  - Petrović, Jelena
PY  - 2023
UR  - https://esleepeurope.eu/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6243
AB  - Background: The GABAergic mechanism is an important target for the action of anesthetics and the promotion of sleep. We investigated the changes in
hippocampal and reticulo-thalamic nucleus (RT) GABAergic parvalbumin (PV)-expressing interneurons as possible underlying mechanisms of the different
local cortical and hippocampal EEG microstructures during NREM sleep compared with anesthesia-induced unconsciousness by two anesthetics with
different main mechanisms of action.
Methods: Twenty adult male Wistar rats were implanted for sleep recordings. After 3 hours of sleep recording, half of the rats were anesthetized with
ketamine/diazepam (100 mg/kg, i.p.) and the other half with propofol (100 mg/kg, i.p.). We recorded EEGs of the motor cortex and hippocampus during the
one-hour stable surgical phase of both anesthetics. The EEG microstructures of the motor cortex and hippocampus in local NREM sleep were compared with
their EEG microstructures during 30 minutes of unconsciousness induced by a given anesthetic. At the end of each recording under stable anesthesia, rats
were sacrificed for further PV and postsynaptic density protein 95 (PSD-95) immunohistochemistry.
Results: All three states of unconsciousness differed in motor cortical and hippocampal EEG microstructures (χ2≥9.46;p≤0.01). During propofol-induced
unconsciousness, attenuated delta and augmented sigma/beta amplitudes (z≥-4.13;p≤0.01) were the globally expressed difference, whereas increased
gamma amplitude (z=2.35;p=0.02) was the only difference at the motor-cortical level compared to NREM sleep. During ketamine/diazepam-induced
unconsciousness, attenuated theta (z≥-5.53;p≤10-4) and increased beta/gamma amplitudes (z≥-4.82;p≤10-4) were the globally expressed difference from
NREM sleep. Both anesthesia-induced unconsciousness expressed globally as increased beta amplitude (z≥-4.13;p≤10-3) and increased motor-cortical
gamma amplitude (z≥-4.20;p≤0.02) compared to NREM sleep. In contrast to propofol anesthesia, there was significant suppression of PV expression in the
hippocampus (z≤-2.71;p≤ .01) and RT during ketamine/diazepam anesthesia in all rats, but only in the hippocampus was there an inhibitory/excitatory
imbalance (increased PSD-95 expression).
Conclusions: Although anesthesia and sleep share many neurobiological features, they are distinct states in terms of local EEG microstructure and
underlying GABAergic and molecular substrate in local neuronal networks.
PB  - European Sleep Research Society
C3  - eSLEEP EUROPE; 2023 Oct 4-6; Online
T1  - GABAergic parvalbumin-expressing interneurons underlie distinct local EEG microstructure during different states of unconsciousness in rats
SP  - 2
EP  - 2
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6243
ER  - 

@conference{
author = "Šaponjić, Jasna and Radovanović, Ljiljana and Novaković, Andrea and Petrović, Jelena",
year = "2023",
abstract = "Background: The GABAergic mechanism is an important target for the action of anesthetics and the promotion of sleep. We investigated the changes in
hippocampal and reticulo-thalamic nucleus (RT) GABAergic parvalbumin (PV)-expressing interneurons as possible underlying mechanisms of the different
local cortical and hippocampal EEG microstructures during NREM sleep compared with anesthesia-induced unconsciousness by two anesthetics with
different main mechanisms of action.
Methods: Twenty adult male Wistar rats were implanted for sleep recordings. After 3 hours of sleep recording, half of the rats were anesthetized with
ketamine/diazepam (100 mg/kg, i.p.) and the other half with propofol (100 mg/kg, i.p.). We recorded EEGs of the motor cortex and hippocampus during the
one-hour stable surgical phase of both anesthetics. The EEG microstructures of the motor cortex and hippocampus in local NREM sleep were compared with
their EEG microstructures during 30 minutes of unconsciousness induced by a given anesthetic. At the end of each recording under stable anesthesia, rats
were sacrificed for further PV and postsynaptic density protein 95 (PSD-95) immunohistochemistry.
Results: All three states of unconsciousness differed in motor cortical and hippocampal EEG microstructures (χ2≥9.46;p≤0.01). During propofol-induced
unconsciousness, attenuated delta and augmented sigma/beta amplitudes (z≥-4.13;p≤0.01) were the globally expressed difference, whereas increased
gamma amplitude (z=2.35;p=0.02) was the only difference at the motor-cortical level compared to NREM sleep. During ketamine/diazepam-induced
unconsciousness, attenuated theta (z≥-5.53;p≤10-4) and increased beta/gamma amplitudes (z≥-4.82;p≤10-4) were the globally expressed difference from
NREM sleep. Both anesthesia-induced unconsciousness expressed globally as increased beta amplitude (z≥-4.13;p≤10-3) and increased motor-cortical
gamma amplitude (z≥-4.20;p≤0.02) compared to NREM sleep. In contrast to propofol anesthesia, there was significant suppression of PV expression in the
hippocampus (z≤-2.71;p≤ .01) and RT during ketamine/diazepam anesthesia in all rats, but only in the hippocampus was there an inhibitory/excitatory
imbalance (increased PSD-95 expression).
Conclusions: Although anesthesia and sleep share many neurobiological features, they are distinct states in terms of local EEG microstructure and
underlying GABAergic and molecular substrate in local neuronal networks.",
publisher = "European Sleep Research Society",
journal = "eSLEEP EUROPE; 2023 Oct 4-6; Online",
title = "GABAergic parvalbumin-expressing interneurons underlie distinct local EEG microstructure during different states of unconsciousness in rats",
pages = "2-2",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6243"
}

Šaponjić, J., Radovanović, L., Novaković, A.,& Petrović, J.. (2023). GABAergic parvalbumin-expressing interneurons underlie distinct local EEG microstructure during different states of unconsciousness in rats. in eSLEEP EUROPE; 2023 Oct 4-6; Online
European Sleep Research Society., 2-2.
https://hdl.handle.net/21.15107/rcub_ibiss_6243

Šaponjić J, Radovanović L, Novaković A, Petrović J. GABAergic parvalbumin-expressing interneurons underlie distinct local EEG microstructure during different states of unconsciousness in rats. in eSLEEP EUROPE; 2023 Oct 4-6; Online. 2023;:2-2.
https://hdl.handle.net/21.15107/rcub_ibiss_6243 .

Šaponjić, Jasna, Radovanović, Ljiljana, Novaković, Andrea, Petrović, Jelena, "GABAergic parvalbumin-expressing interneurons underlie distinct local EEG microstructure during different states of unconsciousness in rats" in eSLEEP EUROPE; 2023 Oct 4-6; Online (2023):2-2,
https://hdl.handle.net/21.15107/rcub_ibiss_6243 .

TY  - CONF
AU  - Radovanović, Ljiljana
AU  - Šaponjić, Jasna
AU  - Petrović, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5828
AB  - We investigated the role of hippocampal GABAergic parvalbumin-expressing (PV)
interneurons in spatial and hippocampus-dependent memory abilities in rat models of
Parkinson's disease (PD).
Experiments were performed in adult male Wistar rats, including physiological
controls (n=14) and toxin lesion-induced PD models: PD cholinopathy (n=10),
hemiparkinsonism (n=7), and hemiparkinsonism with PD cholinopathy (n=6).
Behavioral assessments and PV immunohistochemistry were performed 14 and 42
days after lesions. Spatial habituation test and novel object recognition test were used
to assess spatial and hippocampus-dependent short- and long-term recognition
memory.
All experimental groups had no motor impairments during the follow-up period
(X2
≥2.01, p≥0.07). Although hippocampal PV expression remained unchanged over
time in PD cholinopathy (z≥-1.91, p≥0.06), we evidenced impairments in spatial,
short- and long-term recognition memory, but only at day 42 (X2
≥0.38, p=0.83;
t=0.13, p=0.91). In the hemiparkinsonian rats, unchanged hippocampal PV expression
(z≥-1.52, p≥0.14) was followed by impairment in spatial memory (X2
≥2.87, p≥0.22),
but both recognition memories were intact over time (t≥3.16, p≤0.03). In the
hemiparkinsonian rats with PD cholinopathy, long-lasting impairment of spatial
memory (X2
≥0.72, p≥0.22) was followed by delayed short- and long-term impairment
of recognition memory (t=-0.24, p=0.82) along with hippocampal PV suppression
(z=-3.17, p=10-3), which was functionally coupled to impairment of recognition
memory (r=0.52, p=0.04).
Our results suggest that dopaminergic denervation plays an important role in
impairing spatial memory in the prodromal stage of PD, whereas cholinergic
denervation and hippocampal PV suppression impair short- and long-term memory in
a delayed manner in PD cholinopathy and hemiparkinsonism with PD cholinopathy.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - GABAergic parvalbumin-expressing interneurons play a role in memory impairment in rat models of Parkinson's disease
IS  - 49
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5828
ER  - 

@conference{
author = "Radovanović, Ljiljana and Šaponjić, Jasna and Petrović, Jelena",
year = "2023",
abstract = "We investigated the role of hippocampal GABAergic parvalbumin-expressing (PV)
interneurons in spatial and hippocampus-dependent memory abilities in rat models of
Parkinson's disease (PD).
Experiments were performed in adult male Wistar rats, including physiological
controls (n=14) and toxin lesion-induced PD models: PD cholinopathy (n=10),
hemiparkinsonism (n=7), and hemiparkinsonism with PD cholinopathy (n=6).
Behavioral assessments and PV immunohistochemistry were performed 14 and 42
days after lesions. Spatial habituation test and novel object recognition test were used
to assess spatial and hippocampus-dependent short- and long-term recognition
memory.
All experimental groups had no motor impairments during the follow-up period
(X2
≥2.01, p≥0.07). Although hippocampal PV expression remained unchanged over
time in PD cholinopathy (z≥-1.91, p≥0.06), we evidenced impairments in spatial,
short- and long-term recognition memory, but only at day 42 (X2
≥0.38, p=0.83;
t=0.13, p=0.91). In the hemiparkinsonian rats, unchanged hippocampal PV expression
(z≥-1.52, p≥0.14) was followed by impairment in spatial memory (X2
≥2.87, p≥0.22),
but both recognition memories were intact over time (t≥3.16, p≤0.03). In the
hemiparkinsonian rats with PD cholinopathy, long-lasting impairment of spatial
memory (X2
≥0.72, p≥0.22) was followed by delayed short- and long-term impairment
of recognition memory (t=-0.24, p=0.82) along with hippocampal PV suppression
(z=-3.17, p=10-3), which was functionally coupled to impairment of recognition
memory (r=0.52, p=0.04).
Our results suggest that dopaminergic denervation plays an important role in
impairing spatial memory in the prodromal stage of PD, whereas cholinergic
denervation and hippocampal PV suppression impair short- and long-term memory in
a delayed manner in PD cholinopathy and hemiparkinsonism with PD cholinopathy.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "GABAergic parvalbumin-expressing interneurons play a role in memory impairment in rat models of Parkinson's disease",
number = "49",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5828"
}

Radovanović, L., Šaponjić, J.,& Petrović, J.. (2023). GABAergic parvalbumin-expressing interneurons play a role in memory impairment in rat models of Parkinson's disease. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society.(49).
https://hdl.handle.net/21.15107/rcub_ibiss_5828

Radovanović L, Šaponjić J, Petrović J. GABAergic parvalbumin-expressing interneurons play a role in memory impairment in rat models of Parkinson's disease. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;(49).
https://hdl.handle.net/21.15107/rcub_ibiss_5828 .

Radovanović, Ljiljana, Šaponjić, Jasna, Petrović, Jelena, "GABAergic parvalbumin-expressing interneurons play a role in memory impairment in rat models of Parkinson's disease" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia, no. 49 (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_5828 .

TY  - CONF
AU  - Novaković, Andrea
AU  - Radovanović, Ljiljana
AU  - Petrović, Jelena
AU  - Šaponjić, Jasna
PY  - 2023
UR  - https://fensrm2023algarve.pt/scientific-programme/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5810
AB  - We examined the effects of ketamine/diazepam and propofol anesthesia on hippocampal and reticulo-thalamic (RT) GABAergic parvalbumin (PV)-expressing interneurons in the rat.
A total of 20 adult male Wistar rats were divided into two experimental groups - half were anesthetized with ketamine/diazepam (100 mg/kg, Zoletil® 50), and the other half received propofol anesthesia (100 mg/kg; Propofol Lipuro 2% (20mg/ml). We performed immunohistochemistry protocols for PV and postsynaptic density protein 95 (PSD-95) staining on free-floating 40-µm brain slices. We used PSD-95 as an excitatory synaptic marker to test local excitation changes along with changes in PV expression.
Our results show significant suppression of GABAergic PV-expressing interneurons during ketamine/diazepam anesthesia compared with propofol anesthesia, in the dentate gyrus and CA3 regions of the hippocampus, and in the RT. Moreover, this suppression resulted in an increase in PSD-95 expression only in the hippocampus of rats anesthetized with ketamine/diazepam. Topographically distinct effects of propofol anesthesia were not detected.
The observed imbalance between excitation and inhibition at the level of the hippocampus during ketamine/diazepam anesthesia could be a consequence of lower interneuronal GABA activity. Conversely, the topographically uniform expression of PSD-95 during propofol anesthesia together with higher expression of GABAergic interneurons could possibly indicate a stronger effect of GABA-mediated inhibition in the hippocampus compared with ketamine/diazepam anesthesia.
PB  - Federation of European Neuroscience Societies
C3  - FENS Regional Meeting: FRM 2023; 2023 May 3-5; Algarve, Portugal
T1  - Topographical effects of different anesthetics on GABAergic parvalbumin interneurons in rat
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5810
ER  - 

@conference{
author = "Novaković, Andrea and Radovanović, Ljiljana and Petrović, Jelena and Šaponjić, Jasna",
year = "2023",
abstract = "We examined the effects of ketamine/diazepam and propofol anesthesia on hippocampal and reticulo-thalamic (RT) GABAergic parvalbumin (PV)-expressing interneurons in the rat.
A total of 20 adult male Wistar rats were divided into two experimental groups - half were anesthetized with ketamine/diazepam (100 mg/kg, Zoletil® 50), and the other half received propofol anesthesia (100 mg/kg; Propofol Lipuro 2% (20mg/ml). We performed immunohistochemistry protocols for PV and postsynaptic density protein 95 (PSD-95) staining on free-floating 40-µm brain slices. We used PSD-95 as an excitatory synaptic marker to test local excitation changes along with changes in PV expression.
Our results show significant suppression of GABAergic PV-expressing interneurons during ketamine/diazepam anesthesia compared with propofol anesthesia, in the dentate gyrus and CA3 regions of the hippocampus, and in the RT. Moreover, this suppression resulted in an increase in PSD-95 expression only in the hippocampus of rats anesthetized with ketamine/diazepam. Topographically distinct effects of propofol anesthesia were not detected.
The observed imbalance between excitation and inhibition at the level of the hippocampus during ketamine/diazepam anesthesia could be a consequence of lower interneuronal GABA activity. Conversely, the topographically uniform expression of PSD-95 during propofol anesthesia together with higher expression of GABAergic interneurons could possibly indicate a stronger effect of GABA-mediated inhibition in the hippocampus compared with ketamine/diazepam anesthesia.",
publisher = "Federation of European Neuroscience Societies",
journal = "FENS Regional Meeting: FRM 2023; 2023 May 3-5; Algarve, Portugal",
title = "Topographical effects of different anesthetics on GABAergic parvalbumin interneurons in rat",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5810"
}

Novaković, A., Radovanović, L., Petrović, J.,& Šaponjić, J.. (2023). Topographical effects of different anesthetics on GABAergic parvalbumin interneurons in rat. in FENS Regional Meeting: FRM 2023; 2023 May 3-5; Algarve, Portugal
Federation of European Neuroscience Societies..
https://hdl.handle.net/21.15107/rcub_ibiss_5810

Novaković A, Radovanović L, Petrović J, Šaponjić J. Topographical effects of different anesthetics on GABAergic parvalbumin interneurons in rat. in FENS Regional Meeting: FRM 2023; 2023 May 3-5; Algarve, Portugal. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_5810 .

Novaković, Andrea, Radovanović, Ljiljana, Petrović, Jelena, Šaponjić, Jasna, "Topographical effects of different anesthetics on GABAergic parvalbumin interneurons in rat" in FENS Regional Meeting: FRM 2023; 2023 May 3-5; Algarve, Portugal (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_5810 .

TY  - JOUR
AU  - Radovanović, Ljiljana
AU  - Novaković, Andrea
AU  - Petrović, Jelena
AU  - Šaponjić, Jasna
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5547
AB  - We traced the changes in GABAergic parvalbumin (PV)-expressing interneurons of the hippocampus and reticulo-thalamic nucleus (RT) as possible underlying mechanisms of the different local cortical and hippocampal electroencephalographic (EEG) microstructures during the non-rapid-eye movement (NREM) sleep compared with anesthesia-induced unconsciousness by two anesthetics with different main mechanisms of action (ketamine/diazepam versus propofol). After 3 h of recording their sleep, the rats were divided into two experimental groups: one half received ketamine/diazepam anesthesia and the other half received propofol anesthesia. We simultaneously recorded the EEG of the motor cortex and hippocampus during sleep and during 1 h of surgical anesthesia. We performed immunohistochemistry and analyzed the PV and postsynaptic density protein 95 (PSD-95) expression. PV suppression in the hippocampus and at RT underlies the global theta amplitude attenuation and hippocampal gamma augmentation that is a unique feature of ketamine-induced versus propofol-induced unconsciousness and NREM sleep. While PV suppression resulted in an increase in hippocampal PSD-95 expression, there was no imbalance between inhibition and excitation during ketamine/diazepam anesthesia compared with propofol anesthesia in RT. This increased excitation could be a consequence of a lower GABA interneuronal activity and an additional mechanism underlying the unique local EEG microstructure in the hippocampus during ketamine/diazepam anesthesia.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Different Alterations of Hippocampal and Reticulo-Thalamic GABAergic Parvalbumin-Expressing Interneurons Underlie Different States of Unconsciousness
IS  - 7
VL  - 24
DO  - 10.3390/ijms24076769
SP  - 6769
ER  - 

@article{
author = "Radovanović, Ljiljana and Novaković, Andrea and Petrović, Jelena and Šaponjić, Jasna",
year = "2023",
abstract = "We traced the changes in GABAergic parvalbumin (PV)-expressing interneurons of the hippocampus and reticulo-thalamic nucleus (RT) as possible underlying mechanisms of the different local cortical and hippocampal electroencephalographic (EEG) microstructures during the non-rapid-eye movement (NREM) sleep compared with anesthesia-induced unconsciousness by two anesthetics with different main mechanisms of action (ketamine/diazepam versus propofol). After 3 h of recording their sleep, the rats were divided into two experimental groups: one half received ketamine/diazepam anesthesia and the other half received propofol anesthesia. We simultaneously recorded the EEG of the motor cortex and hippocampus during sleep and during 1 h of surgical anesthesia. We performed immunohistochemistry and analyzed the PV and postsynaptic density protein 95 (PSD-95) expression. PV suppression in the hippocampus and at RT underlies the global theta amplitude attenuation and hippocampal gamma augmentation that is a unique feature of ketamine-induced versus propofol-induced unconsciousness and NREM sleep. While PV suppression resulted in an increase in hippocampal PSD-95 expression, there was no imbalance between inhibition and excitation during ketamine/diazepam anesthesia compared with propofol anesthesia in RT. This increased excitation could be a consequence of a lower GABA interneuronal activity and an additional mechanism underlying the unique local EEG microstructure in the hippocampus during ketamine/diazepam anesthesia.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Different Alterations of Hippocampal and Reticulo-Thalamic GABAergic Parvalbumin-Expressing Interneurons Underlie Different States of Unconsciousness",
number = "7",
volume = "24",
doi = "10.3390/ijms24076769",
pages = "6769"
}

Radovanović, L., Novaković, A., Petrović, J.,& Šaponjić, J.. (2023). Different Alterations of Hippocampal and Reticulo-Thalamic GABAergic Parvalbumin-Expressing Interneurons Underlie Different States of Unconsciousness. in International Journal of Molecular Sciences
Basel: MDPI., 24(7), 6769.
https://doi.org/10.3390/ijms24076769

Radovanović L, Novaković A, Petrović J, Šaponjić J. Different Alterations of Hippocampal and Reticulo-Thalamic GABAergic Parvalbumin-Expressing Interneurons Underlie Different States of Unconsciousness. in International Journal of Molecular Sciences. 2023;24(7):6769.
doi:10.3390/ijms24076769 .

Radovanović, Ljiljana, Novaković, Andrea, Petrović, Jelena, Šaponjić, Jasna, "Different Alterations of Hippocampal and Reticulo-Thalamic GABAergic Parvalbumin-Expressing Interneurons Underlie Different States of Unconsciousness" in International Journal of Molecular Sciences, 24, no. 7 (2023):6769,
https://doi.org/10.3390/ijms24076769 . .

TY  - CONF
AU  - Radovanović, Ljiljana
AU  - Šaponjić, Jasna
AU  - Petrović, Jelena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5556
AB  - We investigated the alterations of reticulo-thalamic (RT) GABAergic parvalbumin (PV+) interneurons and synaptic reorganization underlying the altered hippocampal high voltage sleep spindle (HVS) dynamics during REM sleep in the rat
models of Parkinson’s disease (PD). Adult male Wistar rats were implanted for 6h sleep recording during light phase in four
experimental groups: control (implanted controls), PD cholinopathy (bilateral lesion of the nucleus pedunculopontinus
tegmentalis−PPT), hemiparkinsonism (unilateral lesion of the nucleus substantiae nigrae pars compacta−SNpc) and
hemiparkinsonism with PD cholinopathy (unilateral SNpc/bilateral PPT lesion). Following 14 days of the surgical procedure
we differentiated the Wake/NREM/REM 10s epochs, and the HVSs detection and extraction was done automatically (4.1–10
Hz band pass filter, 1s minimum duration) and visually validated. Hippocampal HVS dynamics were analyzed during 1h of
NREM/REM sleep. Alterations of the PV+ interneurons and synaptic re-organization within the RT were determined by the
parvalbumin, MAP2 and PSD-95 immunostaining. REM sleep is a predisposing state for the HVSs induction in all
experimental models of PD neuropathology. Whereas the PD cholinopathy induced the prolongation and higher density of
hippocampal HVSs, the hemiparkinsonism with PD cholinopathy increased the hippocampal HVSs intrinsic frequency during
REM sleep. In contrast to the unaltered PV+ interneurons/partially enhanced MAP2/suppressed PSD-95 expression during
PD cholinopathy, we evidenced the PV+ interneurons reduction/enhanced MAP2/no change of PSD-95 expression in the RT
during hemiparkinsonism with PD cholinopathy. Distinct PV+ interneurons alteration and inhibition/excitation balance in the
RT could be the underlying mechanisms of HVS generation/alteration during REM sleep in the parkinsonian rats.
PB  - Federation of European Neuroscience Societies
C3  - E-book of Abstracts: FENS Forum 2022; 2022 Jul 9-13; Paris, France
T1  - Hippocampal sleep spindle dynamics during REM sleep and their distinct underlying parvalbumin and synaptic proteins expression in the reticulo-thalamic nucleus of the parkinsonian rats
SP  - 2231
EP  - 2232
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5556
ER  - 

@conference{
author = "Radovanović, Ljiljana and Šaponjić, Jasna and Petrović, Jelena",
year = "2022",
abstract = "We investigated the alterations of reticulo-thalamic (RT) GABAergic parvalbumin (PV+) interneurons and synaptic reorganization underlying the altered hippocampal high voltage sleep spindle (HVS) dynamics during REM sleep in the rat
models of Parkinson’s disease (PD). Adult male Wistar rats were implanted for 6h sleep recording during light phase in four
experimental groups: control (implanted controls), PD cholinopathy (bilateral lesion of the nucleus pedunculopontinus
tegmentalis−PPT), hemiparkinsonism (unilateral lesion of the nucleus substantiae nigrae pars compacta−SNpc) and
hemiparkinsonism with PD cholinopathy (unilateral SNpc/bilateral PPT lesion). Following 14 days of the surgical procedure
we differentiated the Wake/NREM/REM 10s epochs, and the HVSs detection and extraction was done automatically (4.1–10
Hz band pass filter, 1s minimum duration) and visually validated. Hippocampal HVS dynamics were analyzed during 1h of
NREM/REM sleep. Alterations of the PV+ interneurons and synaptic re-organization within the RT were determined by the
parvalbumin, MAP2 and PSD-95 immunostaining. REM sleep is a predisposing state for the HVSs induction in all
experimental models of PD neuropathology. Whereas the PD cholinopathy induced the prolongation and higher density of
hippocampal HVSs, the hemiparkinsonism with PD cholinopathy increased the hippocampal HVSs intrinsic frequency during
REM sleep. In contrast to the unaltered PV+ interneurons/partially enhanced MAP2/suppressed PSD-95 expression during
PD cholinopathy, we evidenced the PV+ interneurons reduction/enhanced MAP2/no change of PSD-95 expression in the RT
during hemiparkinsonism with PD cholinopathy. Distinct PV+ interneurons alteration and inhibition/excitation balance in the
RT could be the underlying mechanisms of HVS generation/alteration during REM sleep in the parkinsonian rats.",
publisher = "Federation of European Neuroscience Societies",
journal = "E-book of Abstracts: FENS Forum 2022; 2022 Jul 9-13; Paris, France",
title = "Hippocampal sleep spindle dynamics during REM sleep and their distinct underlying parvalbumin and synaptic proteins expression in the reticulo-thalamic nucleus of the parkinsonian rats",
pages = "2231-2232",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5556"
}

Radovanović, L., Šaponjić, J.,& Petrović, J.. (2022). Hippocampal sleep spindle dynamics during REM sleep and their distinct underlying parvalbumin and synaptic proteins expression in the reticulo-thalamic nucleus of the parkinsonian rats. in E-book of Abstracts: FENS Forum 2022; 2022 Jul 9-13; Paris, France
Federation of European Neuroscience Societies., 2231-2232.
https://hdl.handle.net/21.15107/rcub_ibiss_5556

Radovanović L, Šaponjić J, Petrović J. Hippocampal sleep spindle dynamics during REM sleep and their distinct underlying parvalbumin and synaptic proteins expression in the reticulo-thalamic nucleus of the parkinsonian rats. in E-book of Abstracts: FENS Forum 2022; 2022 Jul 9-13; Paris, France. 2022;:2231-2232.
https://hdl.handle.net/21.15107/rcub_ibiss_5556 .

Radovanović, Ljiljana, Šaponjić, Jasna, Petrović, Jelena, "Hippocampal sleep spindle dynamics during REM sleep and their distinct underlying parvalbumin and synaptic proteins expression in the reticulo-thalamic nucleus of the parkinsonian rats" in E-book of Abstracts: FENS Forum 2022; 2022 Jul 9-13; Paris, France (2022):2231-2232,
https://hdl.handle.net/21.15107/rcub_ibiss_5556 .

TY  - JOUR
AU  - Petrović, Jelena
AU  - Radovanović, Ljiljana
AU  - Šaponjić, Jasna
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0166432820306562
UR  - http://www.ncbi.nlm.nih.gov/pubmed/33038348
UR  - https://radar.ibiss.bg.ac.rs/123456789/3908
AB  - We investigated the prodromal alterations of local sleep, particularly the motor cortical and hippocampal sleep, along with spontaneous locomotor activity in the rat models of Parkinson's disease (PD). We performed our experiments in adult, male Wistar rats, chronically implanted for sleep recording and divided into four experimental groups: the control (implanted controls), the bilateral pedunculopontine tegmental nucleus (PPT) lesions (PD cholinopathy), the unilateral substantia nigra pars compacta (SNpc) lesions (hemiparkinsonism) and the unilateral SNpc/bilateral PPT lesions (hemiparkinsonism with PD cholinopathy). We followed their sleep, basal locomotor activity and spatial habituation for 14 days following the surgical procedures. Severe prodromal local sleep disturbances in the hemiparkinsonian rats were expressed as sleep fragmentation and distinct local NREM/REM EEG microstructure alterations in both the motor cortex and the hippocampus. Alongside the state-unrelated role of the dopaminergic control of theta oscillations and NREM/REM related sigma and beta oscillations, we demonstrated that the REM neurochemical regulatory substrate is particularly important in the dopaminergic control of beta oscillations. In addition, hippocampal prodromal sleep disorders in the hemiparkinsonian rats were expressed as NREM/REM fragmentation and the opposite impact of dopaminergic versus cholinergic control of the NREM delta and beta oscillation amplitudes in the hippocampus, likewise in the motor cortex versus the hippocampus. All these distinct prodromal local sleep disorders and the dopaminergic vs. cholinergic impact on NREM/REM EEG microstructure alterations are of fundamental importance for the further development and follow-up of PD-modifying therapies, and for the identification of patients who are at risk of developing PD.
PB  - Elsevier
T2  - Behavioural Brain Research
T1  - Prodromal local sleep disorders in a rat model of Parkinson's disease cholinopathy, hemiparkinsonism and hemiparkinsonism with cholinopathy.
VL  - 397
DO  - 10.1016/j.bbr.2020.112957
SP  - 112957
ER  - 

@article{
author = "Petrović, Jelena and Radovanović, Ljiljana and Šaponjić, Jasna",
year = "2021",
abstract = "We investigated the prodromal alterations of local sleep, particularly the motor cortical and hippocampal sleep, along with spontaneous locomotor activity in the rat models of Parkinson's disease (PD). We performed our experiments in adult, male Wistar rats, chronically implanted for sleep recording and divided into four experimental groups: the control (implanted controls), the bilateral pedunculopontine tegmental nucleus (PPT) lesions (PD cholinopathy), the unilateral substantia nigra pars compacta (SNpc) lesions (hemiparkinsonism) and the unilateral SNpc/bilateral PPT lesions (hemiparkinsonism with PD cholinopathy). We followed their sleep, basal locomotor activity and spatial habituation for 14 days following the surgical procedures. Severe prodromal local sleep disturbances in the hemiparkinsonian rats were expressed as sleep fragmentation and distinct local NREM/REM EEG microstructure alterations in both the motor cortex and the hippocampus. Alongside the state-unrelated role of the dopaminergic control of theta oscillations and NREM/REM related sigma and beta oscillations, we demonstrated that the REM neurochemical regulatory substrate is particularly important in the dopaminergic control of beta oscillations. In addition, hippocampal prodromal sleep disorders in the hemiparkinsonian rats were expressed as NREM/REM fragmentation and the opposite impact of dopaminergic versus cholinergic control of the NREM delta and beta oscillation amplitudes in the hippocampus, likewise in the motor cortex versus the hippocampus. All these distinct prodromal local sleep disorders and the dopaminergic vs. cholinergic impact on NREM/REM EEG microstructure alterations are of fundamental importance for the further development and follow-up of PD-modifying therapies, and for the identification of patients who are at risk of developing PD.",
publisher = "Elsevier",
journal = "Behavioural Brain Research",
title = "Prodromal local sleep disorders in a rat model of Parkinson's disease cholinopathy, hemiparkinsonism and hemiparkinsonism with cholinopathy.",
volume = "397",
doi = "10.1016/j.bbr.2020.112957",
pages = "112957"
}

Petrović, J., Radovanović, L.,& Šaponjić, J.. (2021). Prodromal local sleep disorders in a rat model of Parkinson's disease cholinopathy, hemiparkinsonism and hemiparkinsonism with cholinopathy.. in Behavioural Brain Research
Elsevier., 397, 112957.
https://doi.org/10.1016/j.bbr.2020.112957

Petrović J, Radovanović L, Šaponjić J. Prodromal local sleep disorders in a rat model of Parkinson's disease cholinopathy, hemiparkinsonism and hemiparkinsonism with cholinopathy.. in Behavioural Brain Research. 2021;397:112957.
doi:10.1016/j.bbr.2020.112957 .

Petrović, Jelena, Radovanović, Ljiljana, Šaponjić, Jasna, "Prodromal local sleep disorders in a rat model of Parkinson's disease cholinopathy, hemiparkinsonism and hemiparkinsonism with cholinopathy." in Behavioural Brain Research, 397 (2021):112957,
https://doi.org/10.1016/j.bbr.2020.112957 . .

TY  - JOUR
AU  - Petrović, Jelena
AU  - Radovanović, Ljiljana
AU  - Šaponjić, Jasna
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0166432820306562
UR  - http://www.ncbi.nlm.nih.gov/pubmed/33038348
UR  - https://radar.ibiss.bg.ac.rs/123456789/3908
UR  - https://radar.ibiss.bg.ac.rs/123456789/3909
AB  - We investigated the prodromal alterations of local sleep, particularly the motor cortical and hippocampal sleep, along with spontaneous locomotor activity in the rat models of Parkinson's disease (PD). We performed our experiments in adult, male Wistar rats, chronically implanted for sleep recording and divided into four experimental groups: the control (implanted controls), the bilateral pedunculopontine tegmental nucleus (PPT) lesions (PD cholinopathy), the unilateral substantia nigra pars compacta (SNpc) lesions (hemiparkinsonism) and the unilateral SNpc/bilateral PPT lesions (hemiparkinsonism with PD cholinopathy). We followed their sleep, basal locomotor activity and spatial habituation for 14 days following the surgical procedures. Severe prodromal local sleep disturbances in the hemiparkinsonian rats were expressed as sleep fragmentation and distinct local NREM/REM EEG microstructure alterations in both the motor cortex and the hippocampus. Alongside the state-unrelated role of the dopaminergic control of theta oscillations and NREM/REM related sigma and beta oscillations, we demonstrated that the REM neurochemical regulatory substrate is particularly important in the dopaminergic control of beta oscillations. In addition, hippocampal prodromal sleep disorders in the hemiparkinsonian rats were expressed as NREM/REM fragmentation and the opposite impact of dopaminergic versus cholinergic control of the NREM delta and beta oscillation amplitudes in the hippocampus, likewise in the motor cortex versus the hippocampus. All these distinct prodromal local sleep disorders and the dopaminergic vs. cholinergic impact on NREM/REM EEG microstructure alterations are of fundamental importance for the further development and follow-up of PD-modifying therapies, and for the identification of patients who are at risk of developing PD.
PB  - Elsevier
T2  - Behavioural Brain Research
T1  - Prodromal local sleep disorders in a rat model of Parkinson's disease cholinopathy, hemiparkinsonism and hemiparkinsonism with cholinopathy.
VL  - 397
DO  - 10.1016/j.bbr.2020.112957
SP  - 112957
ER  - 

@article{
author = "Petrović, Jelena and Radovanović, Ljiljana and Šaponjić, Jasna",
year = "2021",
abstract = "We investigated the prodromal alterations of local sleep, particularly the motor cortical and hippocampal sleep, along with spontaneous locomotor activity in the rat models of Parkinson's disease (PD). We performed our experiments in adult, male Wistar rats, chronically implanted for sleep recording and divided into four experimental groups: the control (implanted controls), the bilateral pedunculopontine tegmental nucleus (PPT) lesions (PD cholinopathy), the unilateral substantia nigra pars compacta (SNpc) lesions (hemiparkinsonism) and the unilateral SNpc/bilateral PPT lesions (hemiparkinsonism with PD cholinopathy). We followed their sleep, basal locomotor activity and spatial habituation for 14 days following the surgical procedures. Severe prodromal local sleep disturbances in the hemiparkinsonian rats were expressed as sleep fragmentation and distinct local NREM/REM EEG microstructure alterations in both the motor cortex and the hippocampus. Alongside the state-unrelated role of the dopaminergic control of theta oscillations and NREM/REM related sigma and beta oscillations, we demonstrated that the REM neurochemical regulatory substrate is particularly important in the dopaminergic control of beta oscillations. In addition, hippocampal prodromal sleep disorders in the hemiparkinsonian rats were expressed as NREM/REM fragmentation and the opposite impact of dopaminergic versus cholinergic control of the NREM delta and beta oscillation amplitudes in the hippocampus, likewise in the motor cortex versus the hippocampus. All these distinct prodromal local sleep disorders and the dopaminergic vs. cholinergic impact on NREM/REM EEG microstructure alterations are of fundamental importance for the further development and follow-up of PD-modifying therapies, and for the identification of patients who are at risk of developing PD.",
publisher = "Elsevier",
journal = "Behavioural Brain Research",
title = "Prodromal local sleep disorders in a rat model of Parkinson's disease cholinopathy, hemiparkinsonism and hemiparkinsonism with cholinopathy.",
volume = "397",
doi = "10.1016/j.bbr.2020.112957",
pages = "112957"
}

Petrović, J., Radovanović, L.,& Šaponjić, J.. (2021). Prodromal local sleep disorders in a rat model of Parkinson's disease cholinopathy, hemiparkinsonism and hemiparkinsonism with cholinopathy.. in Behavioural Brain Research
Elsevier., 397, 112957.
https://doi.org/10.1016/j.bbr.2020.112957

Petrović J, Radovanović L, Šaponjić J. Prodromal local sleep disorders in a rat model of Parkinson's disease cholinopathy, hemiparkinsonism and hemiparkinsonism with cholinopathy.. in Behavioural Brain Research. 2021;397:112957.
doi:10.1016/j.bbr.2020.112957 .

Petrović, Jelena, Radovanović, Ljiljana, Šaponjić, Jasna, "Prodromal local sleep disorders in a rat model of Parkinson's disease cholinopathy, hemiparkinsonism and hemiparkinsonism with cholinopathy." in Behavioural Brain Research, 397 (2021):112957,
https://doi.org/10.1016/j.bbr.2020.112957 . .

TY  - JOUR
AU  - Radovanović, Ljiljana
AU  - Petrović, Jelena
AU  - Šaponjić, Jasna
PY  - 2021
UR  - https://www.mdpi.com/1422-0067/22/16/8922
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4460
AB  - We investigated the alterations of hippocampal and reticulo-thalamic (RT) GABAergic parvalbumin (PV) interneurons and their synaptic re-organizations underlying the prodromal local sleep disorders in the distinct rat models of Parkinson’s disease (PD). We demonstrated for the first time that REM sleep is a predisposing state for the high-voltage sleep spindles (HVS) induction in all experimental models of PD, particularly during hippocampal REM sleep in the hemiparkinsonian models. There were the opposite underlying alterations of the hippocampal and RT GABAergic PV+ interneurons along with the distinct MAP2 and PSD-95 expressions. Whereas the PD cholinopathy enhanced the number of PV+ interneurons and suppressed the MAP2/PSD-95 expression, the hemiparkinsonism with PD cholinopathy reduced the number of PV+ interneurons and enhanced the MAP2/PSD-95 expression in the hippocampus. Whereas the PD cholinopathy did not alter PV+ interneurons but partially enhanced MAP2 and suppressed PSD-95 expression remotely in the RT, the hemiparkinsonism with PD cholinopathy reduced the PV+ interneurons, enhanced MAP2, and did not change PSD-95 expression remotely in the RT. Our study demonstrates for the first time an important regulatory role of the hippocampal and RT GABAergic PV+ interneurons and the synaptic protein dynamic alterations in the distinct rat models of PD neuropathology.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Hippocampal and Reticulo-Thalamic Parvalbumin Interneurons and Synaptic Re-Organization during Sleep Disorders in the Rat Models of Parkinson’s Disease Neuropathology
IS  - 16
VL  - 22
DO  - 10.3390/ijms22168922
SP  - 8922
ER  - 

@article{
author = "Radovanović, Ljiljana and Petrović, Jelena and Šaponjić, Jasna",
year = "2021",
abstract = "We investigated the alterations of hippocampal and reticulo-thalamic (RT) GABAergic parvalbumin (PV) interneurons and their synaptic re-organizations underlying the prodromal local sleep disorders in the distinct rat models of Parkinson’s disease (PD). We demonstrated for the first time that REM sleep is a predisposing state for the high-voltage sleep spindles (HVS) induction in all experimental models of PD, particularly during hippocampal REM sleep in the hemiparkinsonian models. There were the opposite underlying alterations of the hippocampal and RT GABAergic PV+ interneurons along with the distinct MAP2 and PSD-95 expressions. Whereas the PD cholinopathy enhanced the number of PV+ interneurons and suppressed the MAP2/PSD-95 expression, the hemiparkinsonism with PD cholinopathy reduced the number of PV+ interneurons and enhanced the MAP2/PSD-95 expression in the hippocampus. Whereas the PD cholinopathy did not alter PV+ interneurons but partially enhanced MAP2 and suppressed PSD-95 expression remotely in the RT, the hemiparkinsonism with PD cholinopathy reduced the PV+ interneurons, enhanced MAP2, and did not change PSD-95 expression remotely in the RT. Our study demonstrates for the first time an important regulatory role of the hippocampal and RT GABAergic PV+ interneurons and the synaptic protein dynamic alterations in the distinct rat models of PD neuropathology.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Hippocampal and Reticulo-Thalamic Parvalbumin Interneurons and Synaptic Re-Organization during Sleep Disorders in the Rat Models of Parkinson’s Disease Neuropathology",
number = "16",
volume = "22",
doi = "10.3390/ijms22168922",
pages = "8922"
}

Radovanović, L., Petrović, J.,& Šaponjić, J.. (2021). Hippocampal and Reticulo-Thalamic Parvalbumin Interneurons and Synaptic Re-Organization during Sleep Disorders in the Rat Models of Parkinson’s Disease Neuropathology. in International Journal of Molecular Sciences
Basel: MDPI., 22(16), 8922.
https://doi.org/10.3390/ijms22168922

Radovanović L, Petrović J, Šaponjić J. Hippocampal and Reticulo-Thalamic Parvalbumin Interneurons and Synaptic Re-Organization during Sleep Disorders in the Rat Models of Parkinson’s Disease Neuropathology. in International Journal of Molecular Sciences. 2021;22(16):8922.
doi:10.3390/ijms22168922 .

Radovanović, Ljiljana, Petrović, Jelena, Šaponjić, Jasna, "Hippocampal and Reticulo-Thalamic Parvalbumin Interneurons and Synaptic Re-Organization during Sleep Disorders in the Rat Models of Parkinson’s Disease Neuropathology" in International Journal of Molecular Sciences, 22, no. 16 (2021):8922,
https://doi.org/10.3390/ijms22168922 . .

TY  - JOUR
AU  - Petrović, Jelena
AU  - Radovanović, Ljiljana
AU  - Šaponjić, Jasna
PY  - 2020
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1111/jsr.13090
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32472657
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3696
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3707
AB  - We investigated the homogeneity/heterogeneity of spontaneous sleep, simultaneously recorded in the motor cortex and the hippocampus of control rats, and particularly analysed simultaneous and non-simultaneous motor cortical and hippocampal non-rapid eye movement (NREM)/rapid eye movement (REM) sleep. We demonstrate that the sleep architectures of the motor cortex and hippocampus are different in control rats. There was an increase of NREM duration and a decrease of REM duration in the hippocampus versus the motor cortex. In terms of duration, NREM state is the most heterogeneous in the hippocampus, whereas the REM state is the most heterogeneous in the motor cortex. Whereas the hippocampal NREM duration was increased due to the prolongation of NREM episodes, the hippocampal REM duration decreased due to the decreased number of REM episodes. The heterogeneity of sleep in the motor cortex and hippocampus in control rats was particularly expressed through the inverse alteration of sigma amplitude during NREM sleep and beta/gamma amplitudes during REM sleep in the hippocampus, along with the delta, sigma, beta and gamma amplitudes only during non-simultaneous NREM/REM sleep in the hippocampus. We demonstrated the brain structure-related and NREM/REM state-related heterogeneity of the motor cortical and hippocampal local sleep in control rats. The distinctly altered local NREM/REM states, alongside their episode dynamics and electroencephalographic (EEG) microstructures, suggest the importance of both the local neuronal network substrate and the NREM/REM neurochemical substrate in the control mechanisms of sleep.
PB  - Blackwell Publishing Ltd
T2  - Journal of Sleep Research
T1  - Diversity of simultaneous sleep in the motor cortex and hippocampus in rats.
DO  - 10.1111/jsr.13090
SP  - e13090
ER  - 

@article{
author = "Petrović, Jelena and Radovanović, Ljiljana and Šaponjić, Jasna",
year = "2020",
abstract = "We investigated the homogeneity/heterogeneity of spontaneous sleep, simultaneously recorded in the motor cortex and the hippocampus of control rats, and particularly analysed simultaneous and non-simultaneous motor cortical and hippocampal non-rapid eye movement (NREM)/rapid eye movement (REM) sleep. We demonstrate that the sleep architectures of the motor cortex and hippocampus are different in control rats. There was an increase of NREM duration and a decrease of REM duration in the hippocampus versus the motor cortex. In terms of duration, NREM state is the most heterogeneous in the hippocampus, whereas the REM state is the most heterogeneous in the motor cortex. Whereas the hippocampal NREM duration was increased due to the prolongation of NREM episodes, the hippocampal REM duration decreased due to the decreased number of REM episodes. The heterogeneity of sleep in the motor cortex and hippocampus in control rats was particularly expressed through the inverse alteration of sigma amplitude during NREM sleep and beta/gamma amplitudes during REM sleep in the hippocampus, along with the delta, sigma, beta and gamma amplitudes only during non-simultaneous NREM/REM sleep in the hippocampus. We demonstrated the brain structure-related and NREM/REM state-related heterogeneity of the motor cortical and hippocampal local sleep in control rats. The distinctly altered local NREM/REM states, alongside their episode dynamics and electroencephalographic (EEG) microstructures, suggest the importance of both the local neuronal network substrate and the NREM/REM neurochemical substrate in the control mechanisms of sleep.",
publisher = "Blackwell Publishing Ltd",
journal = "Journal of Sleep Research",
title = "Diversity of simultaneous sleep in the motor cortex and hippocampus in rats.",
doi = "10.1111/jsr.13090",
pages = "e13090"
}

Petrović, J., Radovanović, L.,& Šaponjić, J.. (2020). Diversity of simultaneous sleep in the motor cortex and hippocampus in rats.. in Journal of Sleep Research
Blackwell Publishing Ltd., e13090.
https://doi.org/10.1111/jsr.13090

Petrović J, Radovanović L, Šaponjić J. Diversity of simultaneous sleep in the motor cortex and hippocampus in rats.. in Journal of Sleep Research. 2020;:e13090.
doi:10.1111/jsr.13090 .

Petrović, Jelena, Radovanović, Ljiljana, Šaponjić, Jasna, "Diversity of simultaneous sleep in the motor cortex and hippocampus in rats." in Journal of Sleep Research (2020):e13090,
https://doi.org/10.1111/jsr.13090 . .

TY  - JOUR
AU  - Petrović, Jelena
AU  - Radovanović, Ljiljana
AU  - Šaponjić, Jasna
PY  - 2020
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1111/jsr.13090
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32472657
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3696
UR  - https://radar.ibiss.bg.ac.rs/handle/handle/123456789/3707
AB  - We investigated the homogeneity/heterogeneity of spontaneous sleep, simultaneously recorded in the motor cortex and the hippocampus of control rats, and particularly analysed simultaneous and non-simultaneous motor cortical and hippocampal non-rapid eye movement (NREM)/rapid eye movement (REM) sleep. We demonstrate that the sleep architectures of the motor cortex and hippocampus are different in control rats. There was an increase of NREM duration and a decrease of REM duration in the hippocampus versus the motor cortex. In terms of duration, NREM state is the most heterogeneous in the hippocampus, whereas the REM state is the most heterogeneous in the motor cortex. Whereas the hippocampal NREM duration was increased due to the prolongation of NREM episodes, the hippocampal REM duration decreased due to the decreased number of REM episodes. The heterogeneity of sleep in the motor cortex and hippocampus in control rats was particularly expressed through the inverse alteration of sigma amplitude during NREM sleep and beta/gamma amplitudes during REM sleep in the hippocampus, along with the delta, sigma, beta and gamma amplitudes only during non-simultaneous NREM/REM sleep in the hippocampus. We demonstrated the brain structure-related and NREM/REM state-related heterogeneity of the motor cortical and hippocampal local sleep in control rats. The distinctly altered local NREM/REM states, alongside their episode dynamics and electroencephalographic (EEG) microstructures, suggest the importance of both the local neuronal network substrate and the NREM/REM neurochemical substrate in the control mechanisms of sleep.
PB  - Blackwell Publishing Ltd
T2  - Journal of Sleep Research
T1  - Diversity of simultaneous sleep in the motor cortex and hippocampus in rats.
DO  - 10.1111/jsr.13090
SP  - e13090
ER  - 

@article{
author = "Petrović, Jelena and Radovanović, Ljiljana and Šaponjić, Jasna",
year = "2020",
abstract = "We investigated the homogeneity/heterogeneity of spontaneous sleep, simultaneously recorded in the motor cortex and the hippocampus of control rats, and particularly analysed simultaneous and non-simultaneous motor cortical and hippocampal non-rapid eye movement (NREM)/rapid eye movement (REM) sleep. We demonstrate that the sleep architectures of the motor cortex and hippocampus are different in control rats. There was an increase of NREM duration and a decrease of REM duration in the hippocampus versus the motor cortex. In terms of duration, NREM state is the most heterogeneous in the hippocampus, whereas the REM state is the most heterogeneous in the motor cortex. Whereas the hippocampal NREM duration was increased due to the prolongation of NREM episodes, the hippocampal REM duration decreased due to the decreased number of REM episodes. The heterogeneity of sleep in the motor cortex and hippocampus in control rats was particularly expressed through the inverse alteration of sigma amplitude during NREM sleep and beta/gamma amplitudes during REM sleep in the hippocampus, along with the delta, sigma, beta and gamma amplitudes only during non-simultaneous NREM/REM sleep in the hippocampus. We demonstrated the brain structure-related and NREM/REM state-related heterogeneity of the motor cortical and hippocampal local sleep in control rats. The distinctly altered local NREM/REM states, alongside their episode dynamics and electroencephalographic (EEG) microstructures, suggest the importance of both the local neuronal network substrate and the NREM/REM neurochemical substrate in the control mechanisms of sleep.",
publisher = "Blackwell Publishing Ltd",
journal = "Journal of Sleep Research",
title = "Diversity of simultaneous sleep in the motor cortex and hippocampus in rats.",
doi = "10.1111/jsr.13090",
pages = "e13090"
}

Petrović, J., Radovanović, L.,& Šaponjić, J.. (2020). Diversity of simultaneous sleep in the motor cortex and hippocampus in rats.. in Journal of Sleep Research
Blackwell Publishing Ltd., e13090.
https://doi.org/10.1111/jsr.13090

Petrović J, Radovanović L, Šaponjić J. Diversity of simultaneous sleep in the motor cortex and hippocampus in rats.. in Journal of Sleep Research. 2020;:e13090.
doi:10.1111/jsr.13090 .

Petrović, Jelena, Radovanović, Ljiljana, Šaponjić, Jasna, "Diversity of simultaneous sleep in the motor cortex and hippocampus in rats." in Journal of Sleep Research (2020):e13090,
https://doi.org/10.1111/jsr.13090 . .