TY  - JOUR
AU  - Miljković, Đorđe
AU  - Blaževski, Jana
AU  - Petković, Filip
AU  - Nikolovski, Neda
AU  - Momčilović, Miljana
AU  - Stanisavljević, Suzana
AU  - Jevtić, Bojan
AU  - Stojkovic, Marija Mostarica
AU  - Spasojevic, Ivan
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1987
AB  - Dimethyl fumarate (DMF), a new drug for multiple sclerosis (MS)
   treatment, acts against neuroinflammation via mechanisms that are
   triggered by adduct formation with thiol redox switches. Ethyl pyruvate
   (EP), an off-the-shelf agent, appears to be a redox analog of DMF, but
   its immunomodulatory properties have not been put into the context of MS
   therapy. In this article, we examined and compared the effects of EP and
   DMF on MS-relevant activity/functions of T cells, macrophages,
   microglia, and astrocytes. EP efficiently suppressed the release of MS
   signature cytokines, IFN-gamma and IL-17, from human PBMCs. Furthermore,
   the production of these cytokines was notably decreased in
   encephalitogenic T cells after in vivo application of EP to rats.
   Production of two other proinflammatory cytokines, IL-6 and TNF, and NO
   was suppressed by EP in macrophages and microglia. Reactive oxygen
   species production in macrophages, microglia activation, and the
   development of Ag-presenting phenotype in microglia and macrophages were
   constrained by EP. The release of IL-6 was reduced in astrocytes.
   Finally, EP inhibited the activation of transcription factor NF-kappa B
   in microglia and astrocytes. Most of these effects were also found for
   DMF, implying that EP and DMF share common targets and mechanisms of
   action. Importantly, EP had in vivo impact on experimental autoimmune
   encephalomyelitis, an animal model of MS. Treatment with EP resulted in
   delay and shortening of the first relapse, and lower clinical scores,
   whereas the second attack was annihilated. Further studies on the
   possibility to use EP as an MS therapeutic are warranted.
T2  - Journal of Immunology
T1  - A Comparative Analysis of Multiple Sclerosis-Relevant Anti-Inflammatory
 Properties of Ethyl Pyruvate and Dimethyl Fumarate
IS  - 6
VL  - 194
DO  - 10.4049/jimmunol.1402302
SP  - 2493
EP  - 2503
ER  - 

@article{
author = "Miljković, Đorđe and Blaževski, Jana and Petković, Filip and Nikolovski, Neda and Momčilović, Miljana and Stanisavljević, Suzana and Jevtić, Bojan and Stojkovic, Marija Mostarica and Spasojevic, Ivan",
year = "2015",
abstract = "Dimethyl fumarate (DMF), a new drug for multiple sclerosis (MS)
   treatment, acts against neuroinflammation via mechanisms that are
   triggered by adduct formation with thiol redox switches. Ethyl pyruvate
   (EP), an off-the-shelf agent, appears to be a redox analog of DMF, but
   its immunomodulatory properties have not been put into the context of MS
   therapy. In this article, we examined and compared the effects of EP and
   DMF on MS-relevant activity/functions of T cells, macrophages,
   microglia, and astrocytes. EP efficiently suppressed the release of MS
   signature cytokines, IFN-gamma and IL-17, from human PBMCs. Furthermore,
   the production of these cytokines was notably decreased in
   encephalitogenic T cells after in vivo application of EP to rats.
   Production of two other proinflammatory cytokines, IL-6 and TNF, and NO
   was suppressed by EP in macrophages and microglia. Reactive oxygen
   species production in macrophages, microglia activation, and the
   development of Ag-presenting phenotype in microglia and macrophages were
   constrained by EP. The release of IL-6 was reduced in astrocytes.
   Finally, EP inhibited the activation of transcription factor NF-kappa B
   in microglia and astrocytes. Most of these effects were also found for
   DMF, implying that EP and DMF share common targets and mechanisms of
   action. Importantly, EP had in vivo impact on experimental autoimmune
   encephalomyelitis, an animal model of MS. Treatment with EP resulted in
   delay and shortening of the first relapse, and lower clinical scores,
   whereas the second attack was annihilated. Further studies on the
   possibility to use EP as an MS therapeutic are warranted.",
journal = "Journal of Immunology",
title = "A Comparative Analysis of Multiple Sclerosis-Relevant Anti-Inflammatory
 Properties of Ethyl Pyruvate and Dimethyl Fumarate",
number = "6",
volume = "194",
doi = "10.4049/jimmunol.1402302",
pages = "2493-2503"
}

Miljković, Đ., Blaževski, J., Petković, F., Nikolovski, N., Momčilović, M., Stanisavljević, S., Jevtić, B., Stojkovic, M. M.,& Spasojevic, I.. (2015). A Comparative Analysis of Multiple Sclerosis-Relevant Anti-Inflammatory
 Properties of Ethyl Pyruvate and Dimethyl Fumarate. in Journal of Immunology, 194(6), 2493-2503.
https://doi.org/10.4049/jimmunol.1402302

Miljković Đ, Blaževski J, Petković F, Nikolovski N, Momčilović M, Stanisavljević S, Jevtić B, Stojkovic MM, Spasojevic I. A Comparative Analysis of Multiple Sclerosis-Relevant Anti-Inflammatory
 Properties of Ethyl Pyruvate and Dimethyl Fumarate. in Journal of Immunology. 2015;194(6):2493-2503.
doi:10.4049/jimmunol.1402302 .

Miljković, Đorđe, Blaževski, Jana, Petković, Filip, Nikolovski, Neda, Momčilović, Miljana, Stanisavljević, Suzana, Jevtić, Bojan, Stojkovic, Marija Mostarica, Spasojevic, Ivan, "A Comparative Analysis of Multiple Sclerosis-Relevant Anti-Inflammatory
 Properties of Ethyl Pyruvate and Dimethyl Fumarate" in Journal of Immunology, 194, no. 6 (2015):2493-2503,
https://doi.org/10.4049/jimmunol.1402302 . .

TY  - JOUR
AU  - Nikolić-Kokić, Aleksandra
AU  - Oreščanin Dušić, Zorana
AU  - Spasojevic, Ivan
AU  - Slavic, Marija
AU  - Mijuskovic, Ana
AU  - Paskulin, Roman
AU  - Miljevic, Cedo
AU  - Spasić, Mihajlo
AU  - Blagojević, Duško
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1970
AB  - Ethnopharmacological relevance: Ibogaine is a naturally occurring
   alkaloid with psychotropic and metabotropic effects, derived from the
   bark of the root of the West African Tabernanthe iboga plant. The tribes
   of Kongo basin have been using iboga as a stimulant, for medicinal
   purposes, and in rite of passage ceremonies, for centuries. Besides, it
   has been found that this drug has anti-addictive effects.
   Aim of the study: Previous studies have demonstrated that ibogaine
   changed the quantity of ATP and energy related enzymes as well as the
   activity of antioxidant enzymes in cells thus altering redox equilibrium
   in a time manner. In this work, the mechanism of its action was further
   studied by measuring the effects of ibogaine in human erythrocytes in
   vitro on ATP liberation, membrane fluidity and antioxidant enzymes
   activity.
   Materials and methods: Heparinized human blood samples were incubated
   with ibogaine (10 and 20 mu M) at 37 degrees C for 1 h. Blood plasma was
   separated by centrifugation and the levels of ATP and uric acid were
   measured 10 mm after the addition of ibogaine using standard kits. The
   activity of copper zinc superoxide dismutase (SOD1), catalase (CAT),
   glutathione peroxidase (GSH-Px) and glutathione reductase (GR) were
   measured in erythrocytes after incubation period. The stability of SOD1
   activity was further tested through in vitro incubation with H2O2 and
   scanning of its electrophoretic profiles. Membrane fluidity was
   determined using an electron paramagnetic resonance spin-labelling
   method.
   Results: Results showed that ibogaine treatment of erythrocytes in vitro
   increased ATP concentration in the blood plasma without changes in
   neither erythrocytes membrane fluidity nor uric acid concentration.
   lbogaine also increased SOD1 activity in erythrocytes at both doses
   applied here. Treatment with 20 mu M also elevated GR activity after in
   vitro incubation at 37 degrees C. Electrophoretic profiles revealed that
   incubation with ibogaine mitigates H2O2 mediated suppression of SOD1
   activity.
   Conclusion: Some of the effects of ibogaine seem to be mediated through
   its influence on energy metabolism, redox active processes and the
   effects of discrete fluctuations of individual reactive oxygen species
   on different levels of enzyme activities. Overall, ibogaine acts as a
   pro-antioxidant by increasing activity of antioxidative enzymes and as
   an adaptagene in oxidative distress. (C) 2015 Published by Elsevier
   Ireland Ltd.
T2  - Journal of Ethnopharmacology
T1  - Ex vivo effects of ibogaine on the activity of antioxidative enzymes in
 human erythrocytes
VL  - 164
DO  - 10.1016/j.jep.2015.01.037
SP  - 64
EP  - 70
ER  - 

@article{
author = "Nikolić-Kokić, Aleksandra and Oreščanin Dušić, Zorana and Spasojevic, Ivan and Slavic, Marija and Mijuskovic, Ana and Paskulin, Roman and Miljevic, Cedo and Spasić, Mihajlo and Blagojević, Duško",
year = "2015",
abstract = "Ethnopharmacological relevance: Ibogaine is a naturally occurring
   alkaloid with psychotropic and metabotropic effects, derived from the
   bark of the root of the West African Tabernanthe iboga plant. The tribes
   of Kongo basin have been using iboga as a stimulant, for medicinal
   purposes, and in rite of passage ceremonies, for centuries. Besides, it
   has been found that this drug has anti-addictive effects.
   Aim of the study: Previous studies have demonstrated that ibogaine
   changed the quantity of ATP and energy related enzymes as well as the
   activity of antioxidant enzymes in cells thus altering redox equilibrium
   in a time manner. In this work, the mechanism of its action was further
   studied by measuring the effects of ibogaine in human erythrocytes in
   vitro on ATP liberation, membrane fluidity and antioxidant enzymes
   activity.
   Materials and methods: Heparinized human blood samples were incubated
   with ibogaine (10 and 20 mu M) at 37 degrees C for 1 h. Blood plasma was
   separated by centrifugation and the levels of ATP and uric acid were
   measured 10 mm after the addition of ibogaine using standard kits. The
   activity of copper zinc superoxide dismutase (SOD1), catalase (CAT),
   glutathione peroxidase (GSH-Px) and glutathione reductase (GR) were
   measured in erythrocytes after incubation period. The stability of SOD1
   activity was further tested through in vitro incubation with H2O2 and
   scanning of its electrophoretic profiles. Membrane fluidity was
   determined using an electron paramagnetic resonance spin-labelling
   method.
   Results: Results showed that ibogaine treatment of erythrocytes in vitro
   increased ATP concentration in the blood plasma without changes in
   neither erythrocytes membrane fluidity nor uric acid concentration.
   lbogaine also increased SOD1 activity in erythrocytes at both doses
   applied here. Treatment with 20 mu M also elevated GR activity after in
   vitro incubation at 37 degrees C. Electrophoretic profiles revealed that
   incubation with ibogaine mitigates H2O2 mediated suppression of SOD1
   activity.
   Conclusion: Some of the effects of ibogaine seem to be mediated through
   its influence on energy metabolism, redox active processes and the
   effects of discrete fluctuations of individual reactive oxygen species
   on different levels of enzyme activities. Overall, ibogaine acts as a
   pro-antioxidant by increasing activity of antioxidative enzymes and as
   an adaptagene in oxidative distress. (C) 2015 Published by Elsevier
   Ireland Ltd.",
journal = "Journal of Ethnopharmacology",
title = "Ex vivo effects of ibogaine on the activity of antioxidative enzymes in
 human erythrocytes",
volume = "164",
doi = "10.1016/j.jep.2015.01.037",
pages = "64-70"
}

Nikolić-Kokić, A., Oreščanin Dušić, Z., Spasojevic, I., Slavic, M., Mijuskovic, A., Paskulin, R., Miljevic, C., Spasić, M.,& Blagojević, D.. (2015). Ex vivo effects of ibogaine on the activity of antioxidative enzymes in
 human erythrocytes. in Journal of Ethnopharmacology, 164, 64-70.
https://doi.org/10.1016/j.jep.2015.01.037

Nikolić-Kokić A, Oreščanin Dušić Z, Spasojevic I, Slavic M, Mijuskovic A, Paskulin R, Miljevic C, Spasić M, Blagojević D. Ex vivo effects of ibogaine on the activity of antioxidative enzymes in
 human erythrocytes. in Journal of Ethnopharmacology. 2015;164:64-70.
doi:10.1016/j.jep.2015.01.037 .

Nikolić-Kokić, Aleksandra, Oreščanin Dušić, Zorana, Spasojevic, Ivan, Slavic, Marija, Mijuskovic, Ana, Paskulin, Roman, Miljevic, Cedo, Spasić, Mihajlo, Blagojević, Duško, "Ex vivo effects of ibogaine on the activity of antioxidative enzymes in
 human erythrocytes" in Journal of Ethnopharmacology, 164 (2015):64-70,
https://doi.org/10.1016/j.jep.2015.01.037 . .

TY  - JOUR
AU  - Ristic, Aleksandar J.
AU  - Savić, Danijela
AU  - Sokic, Dragoslav
AU  - Pristov, Jelena Bogdanovic
AU  - Brkljačić, Jelena
AU  - Bascarevic, Vladimir
AU  - Raicevic, Savo
AU  - Savic, Slobodan
AU  - Spasojevic, Ivan
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1960
AB  - ObjectiveTo examine antioxidative system in hippocampi of patients with
   mesial temporal lobe epilepsy associated with hippocampal sclerosis
   (mTLE-HS).
   MethodsActivity and levels of antioxidative enzymescatalase (CAT),
   glutathione peroxidase (GPx), glutathione reductase (GR), manganese
   superoxide dismutase (MnSOD), and copper-zinc superoxide dismutase
   (CuZnSOD)were assessed in hippocampi of nine pharmacoresistant mTLE-HS
   patients (mean age 37.7{[}standard deviation] 6.6years) who underwent
   amygdalohippocampectomy, and in 10 hippocampi obtained via autopsy from
   five neurologically intact controls (mean age 34.49.0years). Subfield
   and cellular (neuron/astrocyte) distribution of CAT, GPx, and MnSOD was
   analyzed in detail using immunohistochemical staining.
   ResultsSclerotic hippocampi showed drastically increased activity of
   hydrogen peroxide-removing enzymes, CAT (p<0.001), GPx (p<0.001), and GR
   (p<0.001), and significantly higher protein levels of CAT (p=0.006), GPx
   (p=0.040), GR (p=0.024), and MnSOD (p=0.004), compared to controls. CAT
   immunofluorescence was located mainly in neurons in both controls and
   HS. Control hippocampi showed GPx staining in blood vessels and CA
   neurons. In HS, GPx-rich loci, representing bundles of astrocytes,
   emerged in different hippocampal regions, whereas the number of
   GPx-positive vessels was drastically decreased. Neurons with abnormal
   morphology and strong MnSOD immunofluorescence were present in all
   neuronal layers in HS. Small autofluorescent deposits, most likely
   lipofuscin, were observed, along with astrogliosis, in CA1 in HS.
   SignificanceAntioxidative system is upregulated in HS. This documents,
   for the first time, that epileptogenic hippocampi are exposed to
   oxidative stress. Our findings provide a basis for understanding the
   potential involvement of redox alterations in the pathology of epilepsy,
   and may open new pharmacologic perspectives for mTLE-HS treatment.
T2  - Epilepsia
T1  - Hippocampal antioxidative system in mesial temporal lobe epilepsy
IS  - 5
VL  - 56
DO  - 10.1111/epi.12981
SP  - 789
EP  - 799
ER  - 

@article{
author = "Ristic, Aleksandar J. and Savić, Danijela and Sokic, Dragoslav and Pristov, Jelena Bogdanovic and Brkljačić, Jelena and Bascarevic, Vladimir and Raicevic, Savo and Savic, Slobodan and Spasojevic, Ivan",
year = "2015",
abstract = "ObjectiveTo examine antioxidative system in hippocampi of patients with
   mesial temporal lobe epilepsy associated with hippocampal sclerosis
   (mTLE-HS).
   MethodsActivity and levels of antioxidative enzymescatalase (CAT),
   glutathione peroxidase (GPx), glutathione reductase (GR), manganese
   superoxide dismutase (MnSOD), and copper-zinc superoxide dismutase
   (CuZnSOD)were assessed in hippocampi of nine pharmacoresistant mTLE-HS
   patients (mean age 37.7{[}standard deviation] 6.6years) who underwent
   amygdalohippocampectomy, and in 10 hippocampi obtained via autopsy from
   five neurologically intact controls (mean age 34.49.0years). Subfield
   and cellular (neuron/astrocyte) distribution of CAT, GPx, and MnSOD was
   analyzed in detail using immunohistochemical staining.
   ResultsSclerotic hippocampi showed drastically increased activity of
   hydrogen peroxide-removing enzymes, CAT (p<0.001), GPx (p<0.001), and GR
   (p<0.001), and significantly higher protein levels of CAT (p=0.006), GPx
   (p=0.040), GR (p=0.024), and MnSOD (p=0.004), compared to controls. CAT
   immunofluorescence was located mainly in neurons in both controls and
   HS. Control hippocampi showed GPx staining in blood vessels and CA
   neurons. In HS, GPx-rich loci, representing bundles of astrocytes,
   emerged in different hippocampal regions, whereas the number of
   GPx-positive vessels was drastically decreased. Neurons with abnormal
   morphology and strong MnSOD immunofluorescence were present in all
   neuronal layers in HS. Small autofluorescent deposits, most likely
   lipofuscin, were observed, along with astrogliosis, in CA1 in HS.
   SignificanceAntioxidative system is upregulated in HS. This documents,
   for the first time, that epileptogenic hippocampi are exposed to
   oxidative stress. Our findings provide a basis for understanding the
   potential involvement of redox alterations in the pathology of epilepsy,
   and may open new pharmacologic perspectives for mTLE-HS treatment.",
journal = "Epilepsia",
title = "Hippocampal antioxidative system in mesial temporal lobe epilepsy",
number = "5",
volume = "56",
doi = "10.1111/epi.12981",
pages = "789-799"
}

Ristic, A. J., Savić, D., Sokic, D., Pristov, J. B., Brkljačić, J., Bascarevic, V., Raicevic, S., Savic, S.,& Spasojevic, I.. (2015). Hippocampal antioxidative system in mesial temporal lobe epilepsy. in Epilepsia, 56(5), 789-799.
https://doi.org/10.1111/epi.12981

Ristic AJ, Savić D, Sokic D, Pristov JB, Brkljačić J, Bascarevic V, Raicevic S, Savic S, Spasojevic I. Hippocampal antioxidative system in mesial temporal lobe epilepsy. in Epilepsia. 2015;56(5):789-799.
doi:10.1111/epi.12981 .

Ristic, Aleksandar J., Savić, Danijela, Sokic, Dragoslav, Pristov, Jelena Bogdanovic, Brkljačić, Jelena, Bascarevic, Vladimir, Raicevic, Savo, Savic, Slobodan, Spasojevic, Ivan, "Hippocampal antioxidative system in mesial temporal lobe epilepsy" in Epilepsia, 56, no. 5 (2015):789-799,
https://doi.org/10.1111/epi.12981 . .

TY  - JOUR
AU  - Milic, Sonja
AU  - Pristov, Jelena Bogdanovic
AU  - Mutavdzic, Dragosav
AU  - Savic, Aleksandar
AU  - Spasić, Mihajlo
AU  - Spasojevic, Ivan
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1971
AB  - Herein we compared antioxidative activities (AA) of 25 free L-amino
   acids (FAA) against Fenton system-mediated hydroxyl radical (HO center
   dot) production in aqueous solution, and examined the relation between
   AA and a set of physicochemical properties. The tank order according -to
   AA was: Tip > notleucine > Phe, Lett > Ile > His
   >3,4-dihydrOxyphenylalanine, Arg > Val > Lys, Tyr, Pro > hydtoxyproline
   > alpha-aminobutyric acid > Gln, Thr, Ser > Glu, Ala, Gly, Asn, Asp.
   Sulfur-containing FAA generated different secondary reactive products,
   which were discriminated by the means of electron paramagnetic resonance
   spin-trapping spectroscopy. AA showed a general positive correlation
   with hydrophobicity. However, when taken separately, uncharged FAA
   exhibited strong positive correlation of AA with hydrophobicity whereas
   charged FAA showed negative or no significant correlation depending on
   the scale applied. A general strong negative correlation was found
   between AA and polarity. Steric parameters and hydration numbers
   correlated positively with AA of rtoripolar side-chain FAA. In addition)
   a decrease of temperature which promotes hydrophobic hydration resulted
   in increased AA. This implies that HO-provoked oxidation of FAA is
   strongly affected by hydrophobic hydration. Our findings are important
   for the understanding of oxidation processes in natural and waste
   waters.
T2  - Environmental Science & Technology
T1  - The Relationship of Physicochemical Properties to the Antioxidative
 Activity of Free Amino Acids in Fenton System
IS  - 7
VL  - 49
DO  - 10.1021/es5053396
SP  - 4245
EP  - 4254
ER  - 

@article{
author = "Milic, Sonja and Pristov, Jelena Bogdanovic and Mutavdzic, Dragosav and Savic, Aleksandar and Spasić, Mihajlo and Spasojevic, Ivan",
year = "2015",
abstract = "Herein we compared antioxidative activities (AA) of 25 free L-amino
   acids (FAA) against Fenton system-mediated hydroxyl radical (HO center
   dot) production in aqueous solution, and examined the relation between
   AA and a set of physicochemical properties. The tank order according -to
   AA was: Tip > notleucine > Phe, Lett > Ile > His
   >3,4-dihydrOxyphenylalanine, Arg > Val > Lys, Tyr, Pro > hydtoxyproline
   > alpha-aminobutyric acid > Gln, Thr, Ser > Glu, Ala, Gly, Asn, Asp.
   Sulfur-containing FAA generated different secondary reactive products,
   which were discriminated by the means of electron paramagnetic resonance
   spin-trapping spectroscopy. AA showed a general positive correlation
   with hydrophobicity. However, when taken separately, uncharged FAA
   exhibited strong positive correlation of AA with hydrophobicity whereas
   charged FAA showed negative or no significant correlation depending on
   the scale applied. A general strong negative correlation was found
   between AA and polarity. Steric parameters and hydration numbers
   correlated positively with AA of rtoripolar side-chain FAA. In addition)
   a decrease of temperature which promotes hydrophobic hydration resulted
   in increased AA. This implies that HO-provoked oxidation of FAA is
   strongly affected by hydrophobic hydration. Our findings are important
   for the understanding of oxidation processes in natural and waste
   waters.",
journal = "Environmental Science & Technology",
title = "The Relationship of Physicochemical Properties to the Antioxidative
 Activity of Free Amino Acids in Fenton System",
number = "7",
volume = "49",
doi = "10.1021/es5053396",
pages = "4245-4254"
}

Milic, S., Pristov, J. B., Mutavdzic, D., Savic, A., Spasić, M.,& Spasojevic, I.. (2015). The Relationship of Physicochemical Properties to the Antioxidative
 Activity of Free Amino Acids in Fenton System. in Environmental Science & Technology, 49(7), 4245-4254.
https://doi.org/10.1021/es5053396

Milic S, Pristov JB, Mutavdzic D, Savic A, Spasić M, Spasojevic I. The Relationship of Physicochemical Properties to the Antioxidative
 Activity of Free Amino Acids in Fenton System. in Environmental Science & Technology. 2015;49(7):4245-4254.
doi:10.1021/es5053396 .

Milic, Sonja, Pristov, Jelena Bogdanovic, Mutavdzic, Dragosav, Savic, Aleksandar, Spasić, Mihajlo, Spasojevic, Ivan, "The Relationship of Physicochemical Properties to the Antioxidative
 Activity of Free Amino Acids in Fenton System" in Environmental Science & Technology, 49, no. 7 (2015):4245-4254,
https://doi.org/10.1021/es5053396 . .

TY  - JOUR
AU  - Bolic, Bojana
AU  - Mijuskovic, Ana
AU  - Popovic-Bijelic, Ana
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasic, Snezana
AU  - Blagojević, Duško
AU  - Spasić, Mihajlo
AU  - Spasojevic, Ivan
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2329
AB  - Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling
   species, with superoxide dimutases (SOD) are poorly understood. We
   applied low-T EPR spectroscopy to examine the effects of HS-/H2S and
   superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and
   CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD
   and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while
   manganese appears to be released from MnSOD active center. Untreated and
   O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems,
   i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of
   (patho)physiological effects of HS-/H2S. (C) 2015 Elsevier Inc. All
   rights reserved.
T2  - Nitric Oxide-Biology and Chemistry
T1  - Reactions of superoxide dismutases with HS-/H2S and superoxide radical
 anion: An in vitro EPR study
VL  - 51
DO  - 10.1016/j.niox.2015.09.008
SP  - 19
EP  - 23
ER  - 

@article{
author = "Bolic, Bojana and Mijuskovic, Ana and Popovic-Bijelic, Ana and Nikolić-Kokić, Aleksandra and Spasic, Snezana and Blagojević, Duško and Spasić, Mihajlo and Spasojevic, Ivan",
year = "2015",
abstract = "Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling
   species, with superoxide dimutases (SOD) are poorly understood. We
   applied low-T EPR spectroscopy to examine the effects of HS-/H2S and
   superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and
   CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD
   and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while
   manganese appears to be released from MnSOD active center. Untreated and
   O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems,
   i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of
   (patho)physiological effects of HS-/H2S. (C) 2015 Elsevier Inc. All
   rights reserved.",
journal = "Nitric Oxide-Biology and Chemistry",
title = "Reactions of superoxide dismutases with HS-/H2S and superoxide radical
 anion: An in vitro EPR study",
volume = "51",
doi = "10.1016/j.niox.2015.09.008",
pages = "19-23"
}

Bolic, B., Mijuskovic, A., Popovic-Bijelic, A., Nikolić-Kokić, A., Spasic, S., Blagojević, D., Spasić, M.,& Spasojevic, I.. (2015). Reactions of superoxide dismutases with HS-/H2S and superoxide radical
 anion: An in vitro EPR study. in Nitric Oxide-Biology and Chemistry, 51, 19-23.
https://doi.org/10.1016/j.niox.2015.09.008

Bolic B, Mijuskovic A, Popovic-Bijelic A, Nikolić-Kokić A, Spasic S, Blagojević D, Spasić M, Spasojevic I. Reactions of superoxide dismutases with HS-/H2S and superoxide radical
 anion: An in vitro EPR study. in Nitric Oxide-Biology and Chemistry. 2015;51:19-23.
doi:10.1016/j.niox.2015.09.008 .

Bolic, Bojana, Mijuskovic, Ana, Popovic-Bijelic, Ana, Nikolić-Kokić, Aleksandra, Spasic, Snezana, Blagojević, Duško, Spasić, Mihajlo, Spasojevic, Ivan, "Reactions of superoxide dismutases with HS-/H2S and superoxide radical
 anion: An in vitro EPR study" in Nitric Oxide-Biology and Chemistry, 51 (2015):19-23,
https://doi.org/10.1016/j.niox.2015.09.008 . .

TY  - CONF
AU  - Mijuskovic, Ana
AU  - Oreščanin Dušić, Zorana
AU  - Nikolić-Kokić, Aleksandra
AU  - Slavic, Marija
AU  - Spasić, Mihajlo
AU  - Spasojevic, Ivan
AU  - Blagojević, Duško
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2211
C3  - Nitric Oxide-Biology and Chemistry
T1  - Sodium sulphide relaxation of rat uterus is related to calcium signaling
IS  - 1
VL  - 39
DO  - 10.1016/j.niox.2014.03.119
SP  - S36
EP  - S37
ER  - 

@conference{
author = "Mijuskovic, Ana and Oreščanin Dušić, Zorana and Nikolić-Kokić, Aleksandra and Slavic, Marija and Spasić, Mihajlo and Spasojevic, Ivan and Blagojević, Duško",
year = "2014",
journal = "Nitric Oxide-Biology and Chemistry",
title = "Sodium sulphide relaxation of rat uterus is related to calcium signaling",
number = "1",
volume = "39",
doi = "10.1016/j.niox.2014.03.119",
pages = "S36-S37"
}

Mijuskovic, A., Oreščanin Dušić, Z., Nikolić-Kokić, A., Slavic, M., Spasić, M., Spasojevic, I.,& Blagojević, D.. (2014). Sodium sulphide relaxation of rat uterus is related to calcium signaling. in Nitric Oxide-Biology and Chemistry, 39(1), S36-S37.
https://doi.org/10.1016/j.niox.2014.03.119

Mijuskovic A, Oreščanin Dušić Z, Nikolić-Kokić A, Slavic M, Spasić M, Spasojevic I, Blagojević D. Sodium sulphide relaxation of rat uterus is related to calcium signaling. in Nitric Oxide-Biology and Chemistry. 2014;39(1):S36-S37.
doi:10.1016/j.niox.2014.03.119 .

Mijuskovic, Ana, Oreščanin Dušić, Zorana, Nikolić-Kokić, Aleksandra, Slavic, Marija, Spasić, Mihajlo, Spasojevic, Ivan, Blagojević, Duško, "Sodium sulphide relaxation of rat uterus is related to calcium signaling" in Nitric Oxide-Biology and Chemistry, 39, no. 1 (2014):S36-S37,
https://doi.org/10.1016/j.niox.2014.03.119 . .

TY  - CONF
AU  - Miljković, Đorđe
AU  - Petković, Filip
AU  - Blaževski, Jana
AU  - Momčilović, Miljana
AU  - Nikolovski, Neda
AU  - Stojkovic, Marija Mostarica
AU  - Spasojevic, Ivan
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2138
C3  - Journal of Neuroimmunology
T1  - Effect of ethyl pyruvate on central nervous system inflammation
IS  - 1-2, SI
VL  - 275
DO  - 10.1016/j.jneuroim.2014.08.600
SP  - 223
EP  - 224
ER  - 

@conference{
author = "Miljković, Đorđe and Petković, Filip and Blaževski, Jana and Momčilović, Miljana and Nikolovski, Neda and Stojkovic, Marija Mostarica and Spasojevic, Ivan",
year = "2014",
journal = "Journal of Neuroimmunology",
title = "Effect of ethyl pyruvate on central nervous system inflammation",
number = "1-2, SI",
volume = "275",
doi = "10.1016/j.jneuroim.2014.08.600",
pages = "223-224"
}

Miljković, Đ., Petković, F., Blaževski, J., Momčilović, M., Nikolovski, N., Stojkovic, M. M.,& Spasojevic, I.. (2014). Effect of ethyl pyruvate on central nervous system inflammation. in Journal of Neuroimmunology, 275(1-2, SI), 223-224.
https://doi.org/10.1016/j.jneuroim.2014.08.600

Miljković Đ, Petković F, Blaževski J, Momčilović M, Nikolovski N, Stojkovic MM, Spasojevic I. Effect of ethyl pyruvate on central nervous system inflammation. in Journal of Neuroimmunology. 2014;275(1-2, SI):223-224.
doi:10.1016/j.jneuroim.2014.08.600 .

Miljković, Đorđe, Petković, Filip, Blaževski, Jana, Momčilović, Miljana, Nikolovski, Neda, Stojkovic, Marija Mostarica, Spasojevic, Ivan, "Effect of ethyl pyruvate on central nervous system inflammation" in Journal of Neuroimmunology, 275, no. 1-2, SI (2014):223-224,
https://doi.org/10.1016/j.jneuroim.2014.08.600 . .

TY  - JOUR
AU  - Ristic, Aleksandar J.
AU  - Sokic, Dragoslav
AU  - Bascarevic, Vladimir
AU  - Spasic, Snezana
AU  - Vojvodic, Nikola
AU  - Savic, Slobodan
AU  - Raicevic, Savo
AU  - Kovacevic, Masa
AU  - Savić, Danijela
AU  - Spasojevic, Ivan
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2214
AB  - An altered metal and electrolyte profile has been implicated in the
   pathologic mechanisms of chronic epilepsy; however, no study has
   comprehensively measured hippocampal concentrations of these elements in
   patients with mesial temporal lobe epilepsy and hippocampal sclerosis
   (mTLE-HS). We therefore analyzed hippocampi of 24 patients with
   drug-resistant mTLE-HS (mean age 35.69.4years) who underwent anterior
   temporal lobe resection and amygdalohippocampectomy and 17 hippocampi
   obtained by autopsy from 13 controls (mean age 40.5 +/- 12.9years),
   using inductively coupled plasma optical emission spectrometry
   (ICP-OES). Epileptic hippocampi showed significantly lower
   concentrations (g/g of tissue) of copper (HS: 2.34 +/- 0.12; control
   {[}C]: 3.57 +/- 0.33; p<0.001), manganese (HS: 0.205 +/- 0.030; C: 0.409
   +/- 0.064; p=0.004), and potassium (HS: 2,001 +/- 59; C: 2,322 +/- 61;
   p<0.001), and increased sodium levels (HS: 1,131 +/- 22; C: 1,040 +/-
   25; p=0.010). Zinc, iron, calcium, and magnesium levels did not differ
   in HS and controls. In summary, copper and manganese levels are
   deficient, whereas iron level is unchanged in hippocampi from patients
   with mTLE-HS. Our results provide a basis for understanding the
   potential involvement of different metals and electrolytes in the
   pathology of HS. A PowerPoint slide summarizing this article is
   available for download in the Supporting Information section .
T2  - Epilepsia
T1  - Metals and electrolytes in sclerotic hippocampi in patients with
 drug-resistant mesial temporal lobe epilepsy
IS  - 5
VL  - 55
DO  - 10.1111/epi.12593
SP  - E34
EP  - E37
ER  - 

@article{
author = "Ristic, Aleksandar J. and Sokic, Dragoslav and Bascarevic, Vladimir and Spasic, Snezana and Vojvodic, Nikola and Savic, Slobodan and Raicevic, Savo and Kovacevic, Masa and Savić, Danijela and Spasojevic, Ivan",
year = "2014",
abstract = "An altered metal and electrolyte profile has been implicated in the
   pathologic mechanisms of chronic epilepsy; however, no study has
   comprehensively measured hippocampal concentrations of these elements in
   patients with mesial temporal lobe epilepsy and hippocampal sclerosis
   (mTLE-HS). We therefore analyzed hippocampi of 24 patients with
   drug-resistant mTLE-HS (mean age 35.69.4years) who underwent anterior
   temporal lobe resection and amygdalohippocampectomy and 17 hippocampi
   obtained by autopsy from 13 controls (mean age 40.5 +/- 12.9years),
   using inductively coupled plasma optical emission spectrometry
   (ICP-OES). Epileptic hippocampi showed significantly lower
   concentrations (g/g of tissue) of copper (HS: 2.34 +/- 0.12; control
   {[}C]: 3.57 +/- 0.33; p<0.001), manganese (HS: 0.205 +/- 0.030; C: 0.409
   +/- 0.064; p=0.004), and potassium (HS: 2,001 +/- 59; C: 2,322 +/- 61;
   p<0.001), and increased sodium levels (HS: 1,131 +/- 22; C: 1,040 +/-
   25; p=0.010). Zinc, iron, calcium, and magnesium levels did not differ
   in HS and controls. In summary, copper and manganese levels are
   deficient, whereas iron level is unchanged in hippocampi from patients
   with mTLE-HS. Our results provide a basis for understanding the
   potential involvement of different metals and electrolytes in the
   pathology of HS. A PowerPoint slide summarizing this article is
   available for download in the Supporting Information section .",
journal = "Epilepsia",
title = "Metals and electrolytes in sclerotic hippocampi in patients with
 drug-resistant mesial temporal lobe epilepsy",
number = "5",
volume = "55",
doi = "10.1111/epi.12593",
pages = "E34-E37"
}

Ristic, A. J., Sokic, D., Bascarevic, V., Spasic, S., Vojvodic, N., Savic, S., Raicevic, S., Kovacevic, M., Savić, D.,& Spasojevic, I.. (2014). Metals and electrolytes in sclerotic hippocampi in patients with
 drug-resistant mesial temporal lobe epilepsy. in Epilepsia, 55(5), E34-E37.
https://doi.org/10.1111/epi.12593

Ristic AJ, Sokic D, Bascarevic V, Spasic S, Vojvodic N, Savic S, Raicevic S, Kovacevic M, Savić D, Spasojevic I. Metals and electrolytes in sclerotic hippocampi in patients with
 drug-resistant mesial temporal lobe epilepsy. in Epilepsia. 2014;55(5):E34-E37.
doi:10.1111/epi.12593 .

Ristic, Aleksandar J., Sokic, Dragoslav, Bascarevic, Vladimir, Spasic, Snezana, Vojvodic, Nikola, Savic, Slobodan, Raicevic, Savo, Kovacevic, Masa, Savić, Danijela, Spasojevic, Ivan, "Metals and electrolytes in sclerotic hippocampi in patients with
 drug-resistant mesial temporal lobe epilepsy" in Epilepsia, 55, no. 5 (2014):E34-E37,
https://doi.org/10.1111/epi.12593 . .

TY  - JOUR
AU  - Mojić, Marija
AU  - Bogdanović Pristov, Jelena
AU  - Maksimović-Ivanić, Danijela
AU  - Jones, David R.
AU  - Stanic, Marina
AU  - Mijatović, Sanja
AU  - Spasojevic, Ivan
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2177
AB  - In vitro studies have shown that hydrogen peroxide (H2O2) produced by
   high-concentration ascorbate and cell culture medium iron efficiently
   kills cancer cells. This provided the rationale for clinical trials of
   high-dose intravenous ascorbate-based treatment for cancer. A drawback
   in all the in vitro studies was their failure to take into account the
   in vivo concentration of iron to supplement cell culture media which are
   characterized by low iron content. Here we showed, using two prostate
   cancer cell lines (LNCaP and PC-3) and primary astrocytes, that the
   anticancer/cytotoxic effects of ascorbate are completely abolished by
   iron at physiological concentrations in cell culture medium and human
   plasma. A detailed examination of mechanisms showed that iron at
   physiological concentrations promotes both production and decomposition
   of H2O2. The latter is mediated by Fenton reaction and prevents H2O2
   accumulation. The hydroxyl radical, which is produced in the Fenton
   reaction, is buffered by extracellular proteins, and could not affect
   intracellular targets like H2O2. These findings show that anticancer
   effects of ascorbate have been significantly overestimated in previous
   in vitro studies, and that common cell culture media might be unsuitable
   for redox research.
T2  - Scientific Reports
T1  - Extracellular iron diminishes anticancer effects of vitamin C: An in
 vitro study
IS  - 5955
VL  - 4
DO  - 10.1038/srep05955
ER  - 

@article{
author = "Mojić, Marija and Bogdanović Pristov, Jelena and Maksimović-Ivanić, Danijela and Jones, David R. and Stanic, Marina and Mijatović, Sanja and Spasojevic, Ivan",
year = "2014",
abstract = "In vitro studies have shown that hydrogen peroxide (H2O2) produced by
   high-concentration ascorbate and cell culture medium iron efficiently
   kills cancer cells. This provided the rationale for clinical trials of
   high-dose intravenous ascorbate-based treatment for cancer. A drawback
   in all the in vitro studies was their failure to take into account the
   in vivo concentration of iron to supplement cell culture media which are
   characterized by low iron content. Here we showed, using two prostate
   cancer cell lines (LNCaP and PC-3) and primary astrocytes, that the
   anticancer/cytotoxic effects of ascorbate are completely abolished by
   iron at physiological concentrations in cell culture medium and human
   plasma. A detailed examination of mechanisms showed that iron at
   physiological concentrations promotes both production and decomposition
   of H2O2. The latter is mediated by Fenton reaction and prevents H2O2
   accumulation. The hydroxyl radical, which is produced in the Fenton
   reaction, is buffered by extracellular proteins, and could not affect
   intracellular targets like H2O2. These findings show that anticancer
   effects of ascorbate have been significantly overestimated in previous
   in vitro studies, and that common cell culture media might be unsuitable
   for redox research.",
journal = "Scientific Reports",
title = "Extracellular iron diminishes anticancer effects of vitamin C: An in
 vitro study",
number = "5955",
volume = "4",
doi = "10.1038/srep05955"
}

Mojić, M., Bogdanović Pristov, J., Maksimović-Ivanić, D., Jones, D. R., Stanic, M., Mijatović, S.,& Spasojevic, I.. (2014). Extracellular iron diminishes anticancer effects of vitamin C: An in
 vitro study. in Scientific Reports, 4(5955).
https://doi.org/10.1038/srep05955

Mojić M, Bogdanović Pristov J, Maksimović-Ivanić D, Jones DR, Stanic M, Mijatović S, Spasojevic I. Extracellular iron diminishes anticancer effects of vitamin C: An in
 vitro study. in Scientific Reports. 2014;4(5955).
doi:10.1038/srep05955 .

Mojić, Marija, Bogdanović Pristov, Jelena, Maksimović-Ivanić, Danijela, Jones, David R., Stanic, Marina, Mijatović, Sanja, Spasojevic, Ivan, "Extracellular iron diminishes anticancer effects of vitamin C: An in
 vitro study" in Scientific Reports, 4, no. 5955 (2014),
https://doi.org/10.1038/srep05955 . .

TY  - JOUR
AU  - Mijuskovic, Ana
AU  - Oreščanin Dušić, Zorana
AU  - Nikolić-Kokić, Aleksandra
AU  - Slavic, Marija
AU  - Spasić, Mihajlo
AU  - Spasojevic, Ivan
AU  - Blagojević, Duško
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2206
AB  - Background: Our aim was to investigate the effect of methanethiol
   (CH3SH) on contractility of rat uterus and activities of redox-active
   enzymes, and to compare them with the effect of sodium sulphide (Na2S),
   a hydrogen sulphide (H2S/HS-) donor.
   Methods: Uteri were isolated from virgin Wistar rats, divided into six
   groups, controls (untreated uteri allowed to contract spontaneously and
   in the presence of Ca2+(6 mM)), CH3SH treated (spontaneously active and
   Ca2+ induced) and Na2S treated (spontaneously active and Ca2+ induced).
   Underlying antioxidative enzyme activities (superoxide dismutase - SOD,
   glutathione peroxidase - GSHPx, glutathione reductase - GR) in CH3SH- or
   Na2S-treated uteri were compared to controls.
   Results: Our experiments showed that CH3SH and Na2S provoked reversible
   relaxation of both spontaneous and Ca2+ induced uterine contractions.
   The dose-response curves differed in shape, and CH3SH curve was shifted
   to higher concentration compared to H2S/HS-. The effects of Na2S fitted
   sigmoid curve, whereas those of CH3SH fitted linearly. CH3SH provoked
   increased SOD activity and decreased GR activity. However, Na2S
   (H2S/HS-) provoked an increase in SOD activity exclusively in Ca2+
   stimulated uteri, while the activity of GSHPx was increased in both
   types of active uteri.
   Conclusion: Our results imply that CH3SH may have a constructive role in
   the control of muscle function and metabolism. Observed differences
   between CH3SH and H2S/HS- could be attributed to a larger moiety that is
   present in CH3SH compared to H2S, but they are more likely to be a
   consequence of the specific actions of HS-, in relation to its negative
   charge. (C) 2014 Institute of Pharmacology, Polish Academy of Sciences.
   Published by Elsevier Urban \& Partner Sp. Z o.o.. All rights reserved.
T2  - Pharmacological Reports
T1  - Comparison of the effects of methanethiol and sodium sulphide on uterine
 contractile activity
IS  - 3
VL  - 66
DO  - 10.1016/j.pharep.2013.12.012
SP  - 373
EP  - 379
ER  - 

@article{
author = "Mijuskovic, Ana and Oreščanin Dušić, Zorana and Nikolić-Kokić, Aleksandra and Slavic, Marija and Spasić, Mihajlo and Spasojevic, Ivan and Blagojević, Duško",
year = "2014",
abstract = "Background: Our aim was to investigate the effect of methanethiol
   (CH3SH) on contractility of rat uterus and activities of redox-active
   enzymes, and to compare them with the effect of sodium sulphide (Na2S),
   a hydrogen sulphide (H2S/HS-) donor.
   Methods: Uteri were isolated from virgin Wistar rats, divided into six
   groups, controls (untreated uteri allowed to contract spontaneously and
   in the presence of Ca2+(6 mM)), CH3SH treated (spontaneously active and
   Ca2+ induced) and Na2S treated (spontaneously active and Ca2+ induced).
   Underlying antioxidative enzyme activities (superoxide dismutase - SOD,
   glutathione peroxidase - GSHPx, glutathione reductase - GR) in CH3SH- or
   Na2S-treated uteri were compared to controls.
   Results: Our experiments showed that CH3SH and Na2S provoked reversible
   relaxation of both spontaneous and Ca2+ induced uterine contractions.
   The dose-response curves differed in shape, and CH3SH curve was shifted
   to higher concentration compared to H2S/HS-. The effects of Na2S fitted
   sigmoid curve, whereas those of CH3SH fitted linearly. CH3SH provoked
   increased SOD activity and decreased GR activity. However, Na2S
   (H2S/HS-) provoked an increase in SOD activity exclusively in Ca2+
   stimulated uteri, while the activity of GSHPx was increased in both
   types of active uteri.
   Conclusion: Our results imply that CH3SH may have a constructive role in
   the control of muscle function and metabolism. Observed differences
   between CH3SH and H2S/HS- could be attributed to a larger moiety that is
   present in CH3SH compared to H2S, but they are more likely to be a
   consequence of the specific actions of HS-, in relation to its negative
   charge. (C) 2014 Institute of Pharmacology, Polish Academy of Sciences.
   Published by Elsevier Urban \& Partner Sp. Z o.o.. All rights reserved.",
journal = "Pharmacological Reports",
title = "Comparison of the effects of methanethiol and sodium sulphide on uterine
 contractile activity",
number = "3",
volume = "66",
doi = "10.1016/j.pharep.2013.12.012",
pages = "373-379"
}

Mijuskovic, A., Oreščanin Dušić, Z., Nikolić-Kokić, A., Slavic, M., Spasić, M., Spasojevic, I.,& Blagojević, D.. (2014). Comparison of the effects of methanethiol and sodium sulphide on uterine
 contractile activity. in Pharmacological Reports, 66(3), 373-379.
https://doi.org/10.1016/j.pharep.2013.12.012

Mijuskovic A, Oreščanin Dušić Z, Nikolić-Kokić A, Slavic M, Spasić M, Spasojevic I, Blagojević D. Comparison of the effects of methanethiol and sodium sulphide on uterine
 contractile activity. in Pharmacological Reports. 2014;66(3):373-379.
doi:10.1016/j.pharep.2013.12.012 .

Mijuskovic, Ana, Oreščanin Dušić, Zorana, Nikolić-Kokić, Aleksandra, Slavic, Marija, Spasić, Mihajlo, Spasojevic, Ivan, Blagojević, Duško, "Comparison of the effects of methanethiol and sodium sulphide on uterine
 contractile activity" in Pharmacological Reports, 66, no. 3 (2014):373-379,
https://doi.org/10.1016/j.pharep.2013.12.012 . .