TY  - JOUR
AU  - Kloska, Anna
AU  - Giełczyk, Agata
AU  - Grzybowski, Tomasz
AU  - Płoski, Rafał
AU  - Kloska, Sylwester
AU  - Marciniak, Tomasz
AU  - Pałczynski, Krzysztof
AU  - Rogalla-Ładniak, Urszula
AU  - Malyarchuk, Boris
AU  - Derenko, Miroslava
AU  - Kovačević-Grujičić, Nataša
AU  - Stevanović, Milena
AU  - Drakulić, Danijela
AU  - Davidović, Slobodan
AU  - Spólnicka, Magdalena
AU  - Zubanska, Magdalena
AU  - Wozniak, Marcin
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6321
AB  - Abstract

Data obtained with the use of massive parallel sequencing (MPS) can be valuable in population genetics studies. In particular, such data harbor the potential for distinguishing samples from different populations, especially from those coming from adjacent populations of common origin. Machine learning (ML) techniques seem to be especially well suited for analyzing large datasets obtained using MPS. The Slavic populations constitute about a third of the population of Europe and inhabit a large area of the continent, while being relatively closely related in population genetics terms. In this proof-of-concept study, various ML techniques were used to classify DNA samples from Slavic and non-Slavic individuals. The primary objective of this study was to empirically evaluate the feasibility of discerning the genetic provenance of individuals of Slavic descent who exhibit genetic similarity, with the overarching goal of categorizing DNA specimens derived from diverse Slavic population representatives. Raw sequencing data were pre-processed, to obtain a 1200 character-long binary vector. A total of three classifiers were used—Random Forest, Support Vector Machine (SVM), and XGBoost. The most-promising results were obtained using SVM with a linear kernel, with 99.9% accuracy and F1-scores of 0.9846–1.000 for all classes.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - A Machine-Learning-Based Approach to Prediction of Biogeographic Ancestry within Europe
IS  - 20
VL  - 24
DO  - 10.3390/ijms242015095
SP  - 15095
ER  - 

@article{
author = "Kloska, Anna and Giełczyk, Agata and Grzybowski, Tomasz and Płoski, Rafał and Kloska, Sylwester and Marciniak, Tomasz and Pałczynski, Krzysztof and Rogalla-Ładniak, Urszula and Malyarchuk, Boris and Derenko, Miroslava and Kovačević-Grujičić, Nataša and Stevanović, Milena and Drakulić, Danijela and Davidović, Slobodan and Spólnicka, Magdalena and Zubanska, Magdalena and Wozniak, Marcin",
year = "2023",
abstract = "Abstract

Data obtained with the use of massive parallel sequencing (MPS) can be valuable in population genetics studies. In particular, such data harbor the potential for distinguishing samples from different populations, especially from those coming from adjacent populations of common origin. Machine learning (ML) techniques seem to be especially well suited for analyzing large datasets obtained using MPS. The Slavic populations constitute about a third of the population of Europe and inhabit a large area of the continent, while being relatively closely related in population genetics terms. In this proof-of-concept study, various ML techniques were used to classify DNA samples from Slavic and non-Slavic individuals. The primary objective of this study was to empirically evaluate the feasibility of discerning the genetic provenance of individuals of Slavic descent who exhibit genetic similarity, with the overarching goal of categorizing DNA specimens derived from diverse Slavic population representatives. Raw sequencing data were pre-processed, to obtain a 1200 character-long binary vector. A total of three classifiers were used—Random Forest, Support Vector Machine (SVM), and XGBoost. The most-promising results were obtained using SVM with a linear kernel, with 99.9% accuracy and F1-scores of 0.9846–1.000 for all classes.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "A Machine-Learning-Based Approach to Prediction of Biogeographic Ancestry within Europe",
number = "20",
volume = "24",
doi = "10.3390/ijms242015095",
pages = "15095"
}

Kloska, A., Giełczyk, A., Grzybowski, T., Płoski, R., Kloska, S., Marciniak, T., Pałczynski, K., Rogalla-Ładniak, U., Malyarchuk, B., Derenko, M., Kovačević-Grujičić, N., Stevanović, M., Drakulić, D., Davidović, S., Spólnicka, M., Zubanska, M.,& Wozniak, M.. (2023). A Machine-Learning-Based Approach to Prediction of Biogeographic Ancestry within Europe. in International Journal of Molecular Sciences
Basel: MDPI., 24(20), 15095.
https://doi.org/10.3390/ijms242015095

Kloska A, Giełczyk A, Grzybowski T, Płoski R, Kloska S, Marciniak T, Pałczynski K, Rogalla-Ładniak U, Malyarchuk B, Derenko M, Kovačević-Grujičić N, Stevanović M, Drakulić D, Davidović S, Spólnicka M, Zubanska M, Wozniak M. A Machine-Learning-Based Approach to Prediction of Biogeographic Ancestry within Europe. in International Journal of Molecular Sciences. 2023;24(20):15095.
doi:10.3390/ijms242015095 .

Kloska, Anna, Giełczyk, Agata, Grzybowski, Tomasz, Płoski, Rafał, Kloska, Sylwester, Marciniak, Tomasz, Pałczynski, Krzysztof, Rogalla-Ładniak, Urszula, Malyarchuk, Boris, Derenko, Miroslava, Kovačević-Grujičić, Nataša, Stevanović, Milena, Drakulić, Danijela, Davidović, Slobodan, Spólnicka, Magdalena, Zubanska, Magdalena, Wozniak, Marcin, "A Machine-Learning-Based Approach to Prediction of Biogeographic Ancestry within Europe" in International Journal of Molecular Sciences, 24, no. 20 (2023):15095,
https://doi.org/10.3390/ijms242015095 . .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Drakulić, Danijela
AU  - Dias, Maria Inês
AU  - Zengin, Gokhan
AU  - Barros, Lillian
AU  - Ivanov, Marija
AU  - Gašić, Uroš
AU  - Rajčević, Nemanja
AU  - Stevanović, Milena
AU  - Ferreira, Isabel C.F.R.
AU  - Soković, Marina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4871
AB  - Despite the significant advances in drug development we are witnessing the inability of health systems to combat both neurodegenerative diseases and cancers, especially glioblastoma. Hence, natural products are comprehen-sively studied in order to provide novel therapeutic options. This study aimed to explore anti-neurodegenerative and anti-glioblastoma potential of extract of Phlomis fruticosa L. using in vitro model systems. It was found that the methanol extract of P. fruticosa was able to efficiently reduce activities of enzymes linked to neurodegenera-tive disease including acetylcholinesterase, butyrylcholinesterase and tyrosinase. Furthermore, P. fruticosa extract has shown excellent antioxidant potential, as evidenced by six different methods. Analysis of cytotoxic effect of P. fruticosa extract on A172 glioblastoma cell line revealed that the concentration of the extract necessary for 50 % inhibition of A172 growth (IC50) was 710 μg/mL. The extract did not induce changes in proliferation and morphology of A172 glioblastoma cells. On the other side, production of ROS was increased in A172 cells treated with the extract. Observed cytotoxic effect of P. fruticosa extract might be based on increase in ROS generation upon treatment. Quantitative chemical analysis revealed the presence of twelve different polyphenols with the cis 3-O-caffeoylquinic acid being the most abundant. This study provided scientific evidence for further exploration of P. fruticosa as a promising natural anti-neurodegenerative therapeutic option.
PB  - Dortmund: EXCLI Journal
T2  - EXCLI Journal
T1  - Phlomis Fruticosa L. Exerts in Vitro Antineurodegenerative and Antioxidant Activities and Induces Prooxidant Effect in Glioblastoma Cell Line
VL  - 21
DO  - 10.17179/excli2021-4487
SP  - 387
EP  - 399
ER  - 

@article{
author = "Stojković, Dejan and Drakulić, Danijela and Dias, Maria Inês and Zengin, Gokhan and Barros, Lillian and Ivanov, Marija and Gašić, Uroš and Rajčević, Nemanja and Stevanović, Milena and Ferreira, Isabel C.F.R. and Soković, Marina",
year = "2022",
abstract = "Despite the significant advances in drug development we are witnessing the inability of health systems to combat both neurodegenerative diseases and cancers, especially glioblastoma. Hence, natural products are comprehen-sively studied in order to provide novel therapeutic options. This study aimed to explore anti-neurodegenerative and anti-glioblastoma potential of extract of Phlomis fruticosa L. using in vitro model systems. It was found that the methanol extract of P. fruticosa was able to efficiently reduce activities of enzymes linked to neurodegenera-tive disease including acetylcholinesterase, butyrylcholinesterase and tyrosinase. Furthermore, P. fruticosa extract has shown excellent antioxidant potential, as evidenced by six different methods. Analysis of cytotoxic effect of P. fruticosa extract on A172 glioblastoma cell line revealed that the concentration of the extract necessary for 50 % inhibition of A172 growth (IC50) was 710 μg/mL. The extract did not induce changes in proliferation and morphology of A172 glioblastoma cells. On the other side, production of ROS was increased in A172 cells treated with the extract. Observed cytotoxic effect of P. fruticosa extract might be based on increase in ROS generation upon treatment. Quantitative chemical analysis revealed the presence of twelve different polyphenols with the cis 3-O-caffeoylquinic acid being the most abundant. This study provided scientific evidence for further exploration of P. fruticosa as a promising natural anti-neurodegenerative therapeutic option.",
publisher = "Dortmund: EXCLI Journal",
journal = "EXCLI Journal",
title = "Phlomis Fruticosa L. Exerts in Vitro Antineurodegenerative and Antioxidant Activities and Induces Prooxidant Effect in Glioblastoma Cell Line",
volume = "21",
doi = "10.17179/excli2021-4487",
pages = "387-399"
}

Stojković, D., Drakulić, D., Dias, M. I., Zengin, G., Barros, L., Ivanov, M., Gašić, U., Rajčević, N., Stevanović, M., Ferreira, I. C.F.R.,& Soković, M.. (2022). Phlomis Fruticosa L. Exerts in Vitro Antineurodegenerative and Antioxidant Activities and Induces Prooxidant Effect in Glioblastoma Cell Line. in EXCLI Journal
Dortmund: EXCLI Journal., 21, 387-399.
https://doi.org/10.17179/excli2021-4487

Stojković D, Drakulić D, Dias MI, Zengin G, Barros L, Ivanov M, Gašić U, Rajčević N, Stevanović M, Ferreira IC, Soković M. Phlomis Fruticosa L. Exerts in Vitro Antineurodegenerative and Antioxidant Activities and Induces Prooxidant Effect in Glioblastoma Cell Line. in EXCLI Journal. 2022;21:387-399.
doi:10.17179/excli2021-4487 .

Stojković, Dejan, Drakulić, Danijela, Dias, Maria Inês, Zengin, Gokhan, Barros, Lillian, Ivanov, Marija, Gašić, Uroš, Rajčević, Nemanja, Stevanović, Milena, Ferreira, Isabel C.F.R., Soković, Marina, "Phlomis Fruticosa L. Exerts in Vitro Antineurodegenerative and Antioxidant Activities and Induces Prooxidant Effect in Glioblastoma Cell Line" in EXCLI Journal, 21 (2022):387-399,
https://doi.org/10.17179/excli2021-4487 . .

TY  - JOUR
AU  - Holub, Karolina
AU  - Malyarchuk, Boris
AU  - Derenko, Miroslava
AU  - Kovačević-Grujičić, Nataša
AU  - Stevanović, Milena
AU  - Drakulić, Danijela
AU  - Davidović, Slobodan
AU  - Grzybowski, Tomasz
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5555
AB  - Genetic markers for the prediction of biogeographical ancestry have proved to be effective tools for law enforcement agencies for many years now. In this study, we attempted to assess the potential of insertion-deletion markers (InDel) and microsatellites (STRs) as subsidiary polymorphisms for inference of Slavic population ancestry. For that purpose, we genotyped Slavic-speaking populations samples from Belarus, the Czech Republic, Poland, Serbia, Ukraine and Russia in 46 InDels and 15 STRs by PCR and capillary electrophoresis and analyzed for between-population differentiation with the use of distance-based methods (FST, principal component analysis and multidimensional scaling).
Additionally, we studied a sample from a Polish individual of well-documented genealogy whose biogeographic ancestry had previously been inferred by commercial genomic services using autosomal single nucleotide polymorphisms (SNPs), mitochondrial DNA and Y-SNP markers. For comparative purposes, we used genotype data collected in the “forInDel” browser and allele frequencies from previously published papers. The results obtained for InDels and STRs show that the Slavic populations constitute a genetically homogeneous group, with the exception of the Czechs differing clearly from the other tested populations. The analysis of the known Polish sample in the Snipper application proves the usefulness of the InDel markers on the continental level only. Conversely, microsatellites not only improve prediction, but are also informative if considered as an independent set of ancestry markers.
PB  - Polish Society of Forensic Medicine and Criminology
T2  - Archives of Forensic Medicine and Criminology
T1  - Verification of insertion-deletion markers (InDels) and microsatellites (STRs) as subsidiary tools for inferring Slavic population ancestry
IS  - 3
VL  - 72
DO  - 10.4467/16891716AMSIK.22.015.17393
SP  - 120
EP  - 137
ER  - 

@article{
author = "Holub, Karolina and Malyarchuk, Boris and Derenko, Miroslava and Kovačević-Grujičić, Nataša and Stevanović, Milena and Drakulić, Danijela and Davidović, Slobodan and Grzybowski, Tomasz",
year = "2022",
abstract = "Genetic markers for the prediction of biogeographical ancestry have proved to be effective tools for law enforcement agencies for many years now. In this study, we attempted to assess the potential of insertion-deletion markers (InDel) and microsatellites (STRs) as subsidiary polymorphisms for inference of Slavic population ancestry. For that purpose, we genotyped Slavic-speaking populations samples from Belarus, the Czech Republic, Poland, Serbia, Ukraine and Russia in 46 InDels and 15 STRs by PCR and capillary electrophoresis and analyzed for between-population differentiation with the use of distance-based methods (FST, principal component analysis and multidimensional scaling).
Additionally, we studied a sample from a Polish individual of well-documented genealogy whose biogeographic ancestry had previously been inferred by commercial genomic services using autosomal single nucleotide polymorphisms (SNPs), mitochondrial DNA and Y-SNP markers. For comparative purposes, we used genotype data collected in the “forInDel” browser and allele frequencies from previously published papers. The results obtained for InDels and STRs show that the Slavic populations constitute a genetically homogeneous group, with the exception of the Czechs differing clearly from the other tested populations. The analysis of the known Polish sample in the Snipper application proves the usefulness of the InDel markers on the continental level only. Conversely, microsatellites not only improve prediction, but are also informative if considered as an independent set of ancestry markers.",
publisher = "Polish Society of Forensic Medicine and Criminology",
journal = "Archives of Forensic Medicine and Criminology",
title = "Verification of insertion-deletion markers (InDels) and microsatellites (STRs) as subsidiary tools for inferring Slavic population ancestry",
number = "3",
volume = "72",
doi = "10.4467/16891716AMSIK.22.015.17393",
pages = "120-137"
}

Holub, K., Malyarchuk, B., Derenko, M., Kovačević-Grujičić, N., Stevanović, M., Drakulić, D., Davidović, S.,& Grzybowski, T.. (2022). Verification of insertion-deletion markers (InDels) and microsatellites (STRs) as subsidiary tools for inferring Slavic population ancestry. in Archives of Forensic Medicine and Criminology
Polish Society of Forensic Medicine and Criminology., 72(3), 120-137.
https://doi.org/10.4467/16891716AMSIK.22.015.17393

Holub K, Malyarchuk B, Derenko M, Kovačević-Grujičić N, Stevanović M, Drakulić D, Davidović S, Grzybowski T. Verification of insertion-deletion markers (InDels) and microsatellites (STRs) as subsidiary tools for inferring Slavic population ancestry. in Archives of Forensic Medicine and Criminology. 2022;72(3):120-137.
doi:10.4467/16891716AMSIK.22.015.17393 .

Holub, Karolina, Malyarchuk, Boris, Derenko, Miroslava, Kovačević-Grujičić, Nataša, Stevanović, Milena, Drakulić, Danijela, Davidović, Slobodan, Grzybowski, Tomasz, "Verification of insertion-deletion markers (InDels) and microsatellites (STRs) as subsidiary tools for inferring Slavic population ancestry" in Archives of Forensic Medicine and Criminology, 72, no. 3 (2022):120-137,
https://doi.org/10.4467/16891716AMSIK.22.015.17393 . .

TY  - JOUR
AU  - Ivanov, Marija
AU  - Božunović, Jelena
AU  - Gašić, Uroš
AU  - Drakulić, Danijela
AU  - Stevanović, Milena
AU  - Rajčević, Nemanja
AU  - Stojković, Dejan
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0926669021010256
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4696
AB  - Salvia nemorosa L. is a rich source of bioactive constituents. We have used different solvents to obtain 14 different extracts in order to evaluate solvents impact on S. nemorosa inflorescences bioactivities. Firstly, the extraction yield, chemical composition and cytotoxic properties towards HaCaT cell line were elaborated. Afterwards, we have studied the extracts bioactivity in terms of wound healing, antioxidant and antidiabetic potentials as well as antimicrobial and antibiofilm capacities; followed by the determination of the antimicrobial mechanism. The extracts yield ranged from 3.03% to 15.75% and they were dominated by epigallocatechin gallate and rosmarinic acid. The majority of extracts did not possess cytotoxic properties towards human keratinocytes. Butanol was the most appropriate solvent for the improvement of wound healing capacity. Combined extraction with ethanol and water resulted in the highest antioxidant potential of the extracts that could be attributed to the high abundance of detected polyphenols. Extraction with butanol led to the increased inhibition towards enzymes linked to type 2-diabetes and increase in antimicrobial capacities. The extracts have shown antibiofilm potential towards Staphylococcus lugdunensis biofilms with more than 90% inhibition for the most active ones. The selected extract EB7 reduced bacterial ability to adhere to human keratinocytes and provided immunomodulatory action in HaCaT cells exposed to S. lugdunensis. The antibacterial mechanism of action of the same extract was associated with moderate perturbation in the cell membrane integrity and with the reduction in bacterial mitochondrial activity. S. nemorosa inflorescences were abundant sources of bioactive polyphenols and with different extraction solvents their bioactivities were further improved. This study reported for the first time the effects of extraction solvents on various bioactivities of S. nemorosa inflorescences, supporting S. nemorosa role as a natural bioactive therapeutic agent with possible antioxidant, antidiabetic and antimicrobial applications.
PB  - Amsterdam: Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Bioactivities of Salvia nemorosa L. inflorescences are influenced by the extraction solvents
VL  - 175
DO  - 10.1016/j.indcrop.2021.114260
SP  - 114260
ER  - 

@article{
author = "Ivanov, Marija and Božunović, Jelena and Gašić, Uroš and Drakulić, Danijela and Stevanović, Milena and Rajčević, Nemanja and Stojković, Dejan",
year = "2022",
abstract = "Salvia nemorosa L. is a rich source of bioactive constituents. We have used different solvents to obtain 14 different extracts in order to evaluate solvents impact on S. nemorosa inflorescences bioactivities. Firstly, the extraction yield, chemical composition and cytotoxic properties towards HaCaT cell line were elaborated. Afterwards, we have studied the extracts bioactivity in terms of wound healing, antioxidant and antidiabetic potentials as well as antimicrobial and antibiofilm capacities; followed by the determination of the antimicrobial mechanism. The extracts yield ranged from 3.03% to 15.75% and they were dominated by epigallocatechin gallate and rosmarinic acid. The majority of extracts did not possess cytotoxic properties towards human keratinocytes. Butanol was the most appropriate solvent for the improvement of wound healing capacity. Combined extraction with ethanol and water resulted in the highest antioxidant potential of the extracts that could be attributed to the high abundance of detected polyphenols. Extraction with butanol led to the increased inhibition towards enzymes linked to type 2-diabetes and increase in antimicrobial capacities. The extracts have shown antibiofilm potential towards Staphylococcus lugdunensis biofilms with more than 90% inhibition for the most active ones. The selected extract EB7 reduced bacterial ability to adhere to human keratinocytes and provided immunomodulatory action in HaCaT cells exposed to S. lugdunensis. The antibacterial mechanism of action of the same extract was associated with moderate perturbation in the cell membrane integrity and with the reduction in bacterial mitochondrial activity. S. nemorosa inflorescences were abundant sources of bioactive polyphenols and with different extraction solvents their bioactivities were further improved. This study reported for the first time the effects of extraction solvents on various bioactivities of S. nemorosa inflorescences, supporting S. nemorosa role as a natural bioactive therapeutic agent with possible antioxidant, antidiabetic and antimicrobial applications.",
publisher = "Amsterdam: Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Bioactivities of Salvia nemorosa L. inflorescences are influenced by the extraction solvents",
volume = "175",
doi = "10.1016/j.indcrop.2021.114260",
pages = "114260"
}

Ivanov, M., Božunović, J., Gašić, U., Drakulić, D., Stevanović, M., Rajčević, N.,& Stojković, D.. (2022). Bioactivities of Salvia nemorosa L. inflorescences are influenced by the extraction solvents. in Industrial Crops and Products
Amsterdam: Elsevier B.V.., 175, 114260.
https://doi.org/10.1016/j.indcrop.2021.114260

Ivanov M, Božunović J, Gašić U, Drakulić D, Stevanović M, Rajčević N, Stojković D. Bioactivities of Salvia nemorosa L. inflorescences are influenced by the extraction solvents. in Industrial Crops and Products. 2022;175:114260.
doi:10.1016/j.indcrop.2021.114260 .

Ivanov, Marija, Božunović, Jelena, Gašić, Uroš, Drakulić, Danijela, Stevanović, Milena, Rajčević, Nemanja, Stojković, Dejan, "Bioactivities of Salvia nemorosa L. inflorescences are influenced by the extraction solvents" in Industrial Crops and Products, 175 (2022):114260,
https://doi.org/10.1016/j.indcrop.2021.114260 . .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Gašić, Uroš
AU  - Drakulić, Danijela
AU  - Zengin, Gokhan
AU  - Stevanović, Milena
AU  - Rajčević, Nemanja
AU  - Soković, Marina
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4229
AB  - Structural diversity of biologically active compounds identified in plants after many years of storage is rarely reported in literature. Herein, we studied chemical profile and biological activities of Phlomis fruticosa L. after plant material storage for 20 years. Chemical analyzes were performed by UHPLC-LTQ-Orbitrap/MS, and revealed presence of 44 compounds: including 13 phenolic acids, 9 phenylethanoids, 20 flavonoids and 2 phenolic related compounds (a phenolic acid derivative and an aliphatic alcohol). The extract showed antimicrobial activity, being the most potent against Aspergillus fumigatus with minimum inhibitory concentration of 0.31 mg/mL. Also, the extract was able to inhibit biofilm formed by Candida species and to inhibit biofilm formation by Staphylococcus aureus. Obtained results revealed that the extract has potential to interfere with the cell membrane permeability of Candida albicans and to suppress production of virulence factor staphyloxanthin in S. aureus. Furthermore, the extract inhibited the activity of α-amylase which is one of the therapeutic targets for diabetes type II. Also, the antiproliferative effect of the extract was demonstrated on human cancer cell lines, while the extract did not exhibit any cytotoxic effect on primary human cells. Based on the obtained results, P. fruticosa could be an interesting source of biologically active compounds even after long term storage.
PB  - Elsevier B.V.
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Chemical profiling, antimicrobial, anti-enzymatic, and cytotoxic properties of Phlomis fruticosa L.
VL  - 195
DO  - 10.1016/j.jpba.2020.113884
SP  - 113884
ER  - 

@article{
author = "Stojković, Dejan and Gašić, Uroš and Drakulić, Danijela and Zengin, Gokhan and Stevanović, Milena and Rajčević, Nemanja and Soković, Marina",
year = "2021",
abstract = "Structural diversity of biologically active compounds identified in plants after many years of storage is rarely reported in literature. Herein, we studied chemical profile and biological activities of Phlomis fruticosa L. after plant material storage for 20 years. Chemical analyzes were performed by UHPLC-LTQ-Orbitrap/MS, and revealed presence of 44 compounds: including 13 phenolic acids, 9 phenylethanoids, 20 flavonoids and 2 phenolic related compounds (a phenolic acid derivative and an aliphatic alcohol). The extract showed antimicrobial activity, being the most potent against Aspergillus fumigatus with minimum inhibitory concentration of 0.31 mg/mL. Also, the extract was able to inhibit biofilm formed by Candida species and to inhibit biofilm formation by Staphylococcus aureus. Obtained results revealed that the extract has potential to interfere with the cell membrane permeability of Candida albicans and to suppress production of virulence factor staphyloxanthin in S. aureus. Furthermore, the extract inhibited the activity of α-amylase which is one of the therapeutic targets for diabetes type II. Also, the antiproliferative effect of the extract was demonstrated on human cancer cell lines, while the extract did not exhibit any cytotoxic effect on primary human cells. Based on the obtained results, P. fruticosa could be an interesting source of biologically active compounds even after long term storage.",
publisher = "Elsevier B.V.",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Chemical profiling, antimicrobial, anti-enzymatic, and cytotoxic properties of Phlomis fruticosa L.",
volume = "195",
doi = "10.1016/j.jpba.2020.113884",
pages = "113884"
}

Stojković, D., Gašić, U., Drakulić, D., Zengin, G., Stevanović, M., Rajčević, N.,& Soković, M.. (2021). Chemical profiling, antimicrobial, anti-enzymatic, and cytotoxic properties of Phlomis fruticosa L.. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier B.V.., 195, 113884.
https://doi.org/10.1016/j.jpba.2020.113884

Stojković D, Gašić U, Drakulić D, Zengin G, Stevanović M, Rajčević N, Soković M. Chemical profiling, antimicrobial, anti-enzymatic, and cytotoxic properties of Phlomis fruticosa L.. in Journal of Pharmaceutical and Biomedical Analysis. 2021;195:113884.
doi:10.1016/j.jpba.2020.113884 .

Stojković, Dejan, Gašić, Uroš, Drakulić, Danijela, Zengin, Gokhan, Stevanović, Milena, Rajčević, Nemanja, Soković, Marina, "Chemical profiling, antimicrobial, anti-enzymatic, and cytotoxic properties of Phlomis fruticosa L." in Journal of Pharmaceutical and Biomedical Analysis, 195 (2021):113884,
https://doi.org/10.1016/j.jpba.2020.113884 . .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Drakulić, Danijela
AU  - Schwirtlich, Marija
AU  - Rajčević, Nemanja
AU  - Stevanović, Milena
AU  - Soković, Marina
AU  - Gašić, Uroš
PY  - 2021
UR  - https://www.mdpi.com/1424-8247/14/1/55
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4209
AB  - Anthriscus cerefolium (L.) Hoffm. is a plant traditionally used around the globe since antiquity. Although widely used in many traditional medicines in different cultures, from the scientific point of view it is poorly investigated. Glioblastoma, a tumor type with poor prognosis, is the most common and lethal brain tumor in adults. Current therapeutic strategies for glioblastoma include surgery, radiation and chemotherapy. On the other hand, it has been revealed that patients with cancers are highly susceptible to microbial infections due to the invasive nature of cancer treatment approaches. This study was designed to investigate the chemical profile of herba Anthriscii cerefoli methanolic extract by applying UHPLC-LTQ OrbiTrap MS4 analysis and to analyze its anti-glioblastoma and antimicrobial activities. This study revealed that methanolic extract of herba Anthrisc cerefolii contained phenolic acids and flavonoids, with 32 compounds being identified. Anti-glioblastoma activity was investigated in vitro using A172 glioblastoma cell line. The cytotoxic effects of the extract on A172 cells were compared to the same effect on primary human gingival fibroblast (HGF-1) cells. Decreased rate of proliferation and changes in cell morphology were detected upon treatment of A172 cells with the extract. The antimicrobial activity of extract was tested against Staphylococcus aureus and Candida species. The extract was active against the tested bacterium and yeasts, inhibiting free floating cells and microbial biofilms. This study is the first one to provide a detailed description of the chemical profile of A. cerefolium extract dealing with scientific insights into its anti-glioblastoma and antimicrobial activities.
PB  - MDPI AG
T2  - Pharmaceuticals
T1  - Extract of Herba Anthrisci cerefolii: Chemical Profiling and Insights into Its Anti-Glioblastoma and Antimicrobial Mechanism of Actions
IS  - 1
VL  - 14
DO  - 10.3390/ph14010055
SP  - 55
ER  - 

@article{
author = "Stojković, Dejan and Drakulić, Danijela and Schwirtlich, Marija and Rajčević, Nemanja and Stevanović, Milena and Soković, Marina and Gašić, Uroš",
year = "2021",
abstract = "Anthriscus cerefolium (L.) Hoffm. is a plant traditionally used around the globe since antiquity. Although widely used in many traditional medicines in different cultures, from the scientific point of view it is poorly investigated. Glioblastoma, a tumor type with poor prognosis, is the most common and lethal brain tumor in adults. Current therapeutic strategies for glioblastoma include surgery, radiation and chemotherapy. On the other hand, it has been revealed that patients with cancers are highly susceptible to microbial infections due to the invasive nature of cancer treatment approaches. This study was designed to investigate the chemical profile of herba Anthriscii cerefoli methanolic extract by applying UHPLC-LTQ OrbiTrap MS4 analysis and to analyze its anti-glioblastoma and antimicrobial activities. This study revealed that methanolic extract of herba Anthrisc cerefolii contained phenolic acids and flavonoids, with 32 compounds being identified. Anti-glioblastoma activity was investigated in vitro using A172 glioblastoma cell line. The cytotoxic effects of the extract on A172 cells were compared to the same effect on primary human gingival fibroblast (HGF-1) cells. Decreased rate of proliferation and changes in cell morphology were detected upon treatment of A172 cells with the extract. The antimicrobial activity of extract was tested against Staphylococcus aureus and Candida species. The extract was active against the tested bacterium and yeasts, inhibiting free floating cells and microbial biofilms. This study is the first one to provide a detailed description of the chemical profile of A. cerefolium extract dealing with scientific insights into its anti-glioblastoma and antimicrobial activities.",
publisher = "MDPI AG",
journal = "Pharmaceuticals",
title = "Extract of Herba Anthrisci cerefolii: Chemical Profiling and Insights into Its Anti-Glioblastoma and Antimicrobial Mechanism of Actions",
number = "1",
volume = "14",
doi = "10.3390/ph14010055",
pages = "55"
}

Stojković, D., Drakulić, D., Schwirtlich, M., Rajčević, N., Stevanović, M., Soković, M.,& Gašić, U.. (2021). Extract of Herba Anthrisci cerefolii: Chemical Profiling and Insights into Its Anti-Glioblastoma and Antimicrobial Mechanism of Actions. in Pharmaceuticals
MDPI AG., 14(1), 55.
https://doi.org/10.3390/ph14010055

Stojković D, Drakulić D, Schwirtlich M, Rajčević N, Stevanović M, Soković M, Gašić U. Extract of Herba Anthrisci cerefolii: Chemical Profiling and Insights into Its Anti-Glioblastoma and Antimicrobial Mechanism of Actions. in Pharmaceuticals. 2021;14(1):55.
doi:10.3390/ph14010055 .

Stojković, Dejan, Drakulić, Danijela, Schwirtlich, Marija, Rajčević, Nemanja, Stevanović, Milena, Soković, Marina, Gašić, Uroš, "Extract of Herba Anthrisci cerefolii: Chemical Profiling and Insights into Its Anti-Glioblastoma and Antimicrobial Mechanism of Actions" in Pharmaceuticals, 14, no. 1 (2021):55,
https://doi.org/10.3390/ph14010055 . .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Dias, Maria Inês
AU  - Drakulić, Danijela
AU  - Barros, Lillian
AU  - Stevanović, Milena
AU  - C F R Ferreira, Isabel
AU  - Soković, Marina
PY  - 2020
UR  - https://www.mdpi.com/1424-8247/13/4/78
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32344670
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC7243113
UR  - https://radar.ibiss.bg.ac.rs/123456789/3854
AB  - Ononis spinosa L. is a plant traditionally used as folk remedy. There are numerous studies regarding chemical constituents and health beneficial properties of Ononidis Radix. The following study was designed to investigate chemical composition and antifungal potential of the methanolic extract obtained from the O. spinosa L. herb. Chemical analyses regarding phenolic compounds of O. spinosa were performed by liquid chromatography with mass spectrometry (LC-DAD-ESI/MSn). Antifungal activity, antibiofilm properties and antifungal mode of action of the extract were evaluated, as well as cytotoxicity. Chemical analyses revealed the presence of flavonoids, isoflavonoids and phenolic acids in O. spinosa, with kaempherol-O-hexoside-pentoside being the most abundant compound (5.1 mg/g extract). Methanolic extract was active against all of the tested microfungi with Penicillium aurantiogriseum being the most sensitive to the extract inhibitory effect at 0.02 mg/mL; and effectively inhibited biofilms formed by Candida strains. Minimum fungicidal concentrations of extract rose in the presence of ergosterol and leakage of cellular components was detected. The extract showed no cytotoxicity to human gingival fibroblast (HGF-1) cells. This study significantly contributes to overall knowledge about medicinal potential of O. spinosa herbal extract and enlightens previously unrevealed properties. O. spinosa aerial parts seem to be an interesting candidate for the development of antifungal preparations, non-toxic to human cells.
PB  - MDPI AG
T2  - Pharmaceuticals (Basel, Switzerland)
T1  - Methanolic Extract of the Herb Ononis spinosa L. Is an Antifungal Agent with no Cytotoxicity to Primary Human Cells.
IS  - 4
VL  - 13
DO  - 10.3390/ph13040078
SP  - 78
ER  - 

@article{
author = "Stojković, Dejan and Dias, Maria Inês and Drakulić, Danijela and Barros, Lillian and Stevanović, Milena and C F R Ferreira, Isabel and Soković, Marina",
year = "2020",
abstract = "Ononis spinosa L. is a plant traditionally used as folk remedy. There are numerous studies regarding chemical constituents and health beneficial properties of Ononidis Radix. The following study was designed to investigate chemical composition and antifungal potential of the methanolic extract obtained from the O. spinosa L. herb. Chemical analyses regarding phenolic compounds of O. spinosa were performed by liquid chromatography with mass spectrometry (LC-DAD-ESI/MSn). Antifungal activity, antibiofilm properties and antifungal mode of action of the extract were evaluated, as well as cytotoxicity. Chemical analyses revealed the presence of flavonoids, isoflavonoids and phenolic acids in O. spinosa, with kaempherol-O-hexoside-pentoside being the most abundant compound (5.1 mg/g extract). Methanolic extract was active against all of the tested microfungi with Penicillium aurantiogriseum being the most sensitive to the extract inhibitory effect at 0.02 mg/mL; and effectively inhibited biofilms formed by Candida strains. Minimum fungicidal concentrations of extract rose in the presence of ergosterol and leakage of cellular components was detected. The extract showed no cytotoxicity to human gingival fibroblast (HGF-1) cells. This study significantly contributes to overall knowledge about medicinal potential of O. spinosa herbal extract and enlightens previously unrevealed properties. O. spinosa aerial parts seem to be an interesting candidate for the development of antifungal preparations, non-toxic to human cells.",
publisher = "MDPI AG",
journal = "Pharmaceuticals (Basel, Switzerland)",
title = "Methanolic Extract of the Herb Ononis spinosa L. Is an Antifungal Agent with no Cytotoxicity to Primary Human Cells.",
number = "4",
volume = "13",
doi = "10.3390/ph13040078",
pages = "78"
}

Stojković, D., Dias, M. I., Drakulić, D., Barros, L., Stevanović, M., C F R Ferreira, I.,& Soković, M.. (2020). Methanolic Extract of the Herb Ononis spinosa L. Is an Antifungal Agent with no Cytotoxicity to Primary Human Cells.. in Pharmaceuticals (Basel, Switzerland)
MDPI AG., 13(4), 78.
https://doi.org/10.3390/ph13040078

Stojković D, Dias MI, Drakulić D, Barros L, Stevanović M, C F R Ferreira I, Soković M. Methanolic Extract of the Herb Ononis spinosa L. Is an Antifungal Agent with no Cytotoxicity to Primary Human Cells.. in Pharmaceuticals (Basel, Switzerland). 2020;13(4):78.
doi:10.3390/ph13040078 .

Stojković, Dejan, Dias, Maria Inês, Drakulić, Danijela, Barros, Lillian, Stevanović, Milena, C F R Ferreira, Isabel, Soković, Marina, "Methanolic Extract of the Herb Ononis spinosa L. Is an Antifungal Agent with no Cytotoxicity to Primary Human Cells." in Pharmaceuticals (Basel, Switzerland), 13, no. 4 (2020):78,
https://doi.org/10.3390/ph13040078 . .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Drakulić, Danijela
AU  - Gašić, Uroš
AU  - Zengin, Gokhan
AU  - Stevanović, Milena
AU  - Rajčević, Nemanja
AU  - Soković, Marina
PY  - 2020
UR  - https://pubs.rsc.org/en/content/articlehtml/2020/fo/d0fo01595d
UR  - https://pubs.rsc.org/en/content/articlelanding/2020/fo/d0fo01595d
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32749435
UR  - https://radar.ibiss.bg.ac.rs/123456789/3853
AB  - This study explored the chemical profile of the aerial parts of Ononis spinosa and further investigated its biological activities. Chemical profiling of the extract revealed the presence of 63 different compounds: phenolic acids, flavonoid glycosides and aglycones, isoflavonoid glycosides and aglycones, and other related compounds. Our results revealed that the extract was active against 8 strains of free floating bacteria. It showed anti-biofilm potential against Staphylococcus aureus and was able to supress the production of staphyloxanthin in S. aureus at sub-minimal inhibitory concentrations. Its antioxidant activity was evaluated by using several assays (phosphomolybdenum, DPPH, ABTS, CUPRAC, FRAP, and metal chelating assay), which showed that the extract exhibited a dose dependent activity. Inhibition of AChE, BChE, amylase, glucosidase and tyrosinase was achieved by the extract, demonstrating its anti-enzymatic activity. The antiproliferative potential of the extract towards human cancer cell lines (HepG2, MCF-7, SiHa and A172) was determined by using the crystal violet assay. Ki67, a marker of proliferation was downregulated in the A172 glioblastoma cell line.
PB  - Royal Society of Chemistry (RSC)
T2  - Food & Function
T1  - Ononis spinosa L., an edible and medicinal plant: UHPLC-LTQ-Orbitrap/MS chemical profiling and biological activities of the herbal extract
IS  - 8
VL  - 11
DO  - 10.1039/d0fo01595d
SP  - 7138
EP  - 7151
ER  - 

@article{
author = "Stojković, Dejan and Drakulić, Danijela and Gašić, Uroš and Zengin, Gokhan and Stevanović, Milena and Rajčević, Nemanja and Soković, Marina",
year = "2020",
abstract = "This study explored the chemical profile of the aerial parts of Ononis spinosa and further investigated its biological activities. Chemical profiling of the extract revealed the presence of 63 different compounds: phenolic acids, flavonoid glycosides and aglycones, isoflavonoid glycosides and aglycones, and other related compounds. Our results revealed that the extract was active against 8 strains of free floating bacteria. It showed anti-biofilm potential against Staphylococcus aureus and was able to supress the production of staphyloxanthin in S. aureus at sub-minimal inhibitory concentrations. Its antioxidant activity was evaluated by using several assays (phosphomolybdenum, DPPH, ABTS, CUPRAC, FRAP, and metal chelating assay), which showed that the extract exhibited a dose dependent activity. Inhibition of AChE, BChE, amylase, glucosidase and tyrosinase was achieved by the extract, demonstrating its anti-enzymatic activity. The antiproliferative potential of the extract towards human cancer cell lines (HepG2, MCF-7, SiHa and A172) was determined by using the crystal violet assay. Ki67, a marker of proliferation was downregulated in the A172 glioblastoma cell line.",
publisher = "Royal Society of Chemistry (RSC)",
journal = "Food & Function",
title = "Ononis spinosa L., an edible and medicinal plant: UHPLC-LTQ-Orbitrap/MS chemical profiling and biological activities of the herbal extract",
number = "8",
volume = "11",
doi = "10.1039/d0fo01595d",
pages = "7138-7151"
}

Stojković, D., Drakulić, D., Gašić, U., Zengin, G., Stevanović, M., Rajčević, N.,& Soković, M.. (2020). Ononis spinosa L., an edible and medicinal plant: UHPLC-LTQ-Orbitrap/MS chemical profiling and biological activities of the herbal extract. in Food & Function
Royal Society of Chemistry (RSC)., 11(8), 7138-7151.
https://doi.org/10.1039/d0fo01595d

Stojković D, Drakulić D, Gašić U, Zengin G, Stevanović M, Rajčević N, Soković M. Ononis spinosa L., an edible and medicinal plant: UHPLC-LTQ-Orbitrap/MS chemical profiling and biological activities of the herbal extract. in Food & Function. 2020;11(8):7138-7151.
doi:10.1039/d0fo01595d .

Stojković, Dejan, Drakulić, Danijela, Gašić, Uroš, Zengin, Gokhan, Stevanović, Milena, Rajčević, Nemanja, Soković, Marina, "Ononis spinosa L., an edible and medicinal plant: UHPLC-LTQ-Orbitrap/MS chemical profiling and biological activities of the herbal extract" in Food & Function, 11, no. 8 (2020):7138-7151,
https://doi.org/10.1039/d0fo01595d . .

TY  - CONF
AU  - Drakulić, Danijela
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Stevanović, Milena
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4370
AB  - Cancer represents a heterogeneous group of diseases characterized by uncontrolled 
proliferation of cells, tissue invasion and metastasis. Despite current therapeutic 
strategies which include a combination of surgery, radiation and chemotherapy, cancer 
is second leading cause of death worldwide next to cardiovascular diseases. Therefore, 
the identification of new drugs with high sensitivity to cancer cells is warranted. The 
anticancer properties of plants and their compounds have been shown since ancient 
times and nowadays, there is a growing interest for identification of new plant-derived 
anticancer agents. 
This study was designed to analyze antiproliferative potential of three different plant 
species, namely Ononis spinosa L. (Fabaceae), Anthriscus cerefolium (L.) Hoffm. (Apiaceae) 
and Phlomis fruticosa L. (Lamiaceae). The aerial parts of plants were collected, dried 
and the plant material was subjected to successive extraction with methanol and 
then evaporated to dryness on a rotary vacuum evaporator. The obtained methanolic 
extracts were used for further analysis. Cytotoxic effect of methanolic extracts on HaCaT 
(spontaneously immortalized human keratinocyte cell line), MCF-7 (breast cancer cell 
line), SiHa (cervical cancer cell line), A172 (glioblastoma cell line) and HepG2 (human 
hepatocellular carcinoma cell line) cells was determined by crystal violet colorimetric 
assay. Antiproliferative activity of methanolic extracts on glioblastoma cells was 
evaluated by Ki67 immunostaining.
The results showed that plant extracts reduced viability of all tested cell lines. The 
most active extract was the one from O. spinosa, indicating by the lowest IC50 value. 
Ki67 immunostaining indicates antiproliferative activity of methanolic extracts on 
glioblastoma cells.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade
C3  - The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia
T1  - Comparative study of antiproliferative potential of three  different plant species
SP  - 38
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4370
ER  - 

@conference{
author = "Drakulić, Danijela and Stojković, Dejan and Soković, Marina and Stevanović, Milena",
year = "2019",
abstract = "Cancer represents a heterogeneous group of diseases characterized by uncontrolled 
proliferation of cells, tissue invasion and metastasis. Despite current therapeutic 
strategies which include a combination of surgery, radiation and chemotherapy, cancer 
is second leading cause of death worldwide next to cardiovascular diseases. Therefore, 
the identification of new drugs with high sensitivity to cancer cells is warranted. The 
anticancer properties of plants and their compounds have been shown since ancient 
times and nowadays, there is a growing interest for identification of new plant-derived 
anticancer agents. 
This study was designed to analyze antiproliferative potential of three different plant 
species, namely Ononis spinosa L. (Fabaceae), Anthriscus cerefolium (L.) Hoffm. (Apiaceae) 
and Phlomis fruticosa L. (Lamiaceae). The aerial parts of plants were collected, dried 
and the plant material was subjected to successive extraction with methanol and 
then evaporated to dryness on a rotary vacuum evaporator. The obtained methanolic 
extracts were used for further analysis. Cytotoxic effect of methanolic extracts on HaCaT 
(spontaneously immortalized human keratinocyte cell line), MCF-7 (breast cancer cell 
line), SiHa (cervical cancer cell line), A172 (glioblastoma cell line) and HepG2 (human 
hepatocellular carcinoma cell line) cells was determined by crystal violet colorimetric 
assay. Antiproliferative activity of methanolic extracts on glioblastoma cells was 
evaluated by Ki67 immunostaining.
The results showed that plant extracts reduced viability of all tested cell lines. The 
most active extract was the one from O. spinosa, indicating by the lowest IC50 value. 
Ki67 immunostaining indicates antiproliferative activity of methanolic extracts on 
glioblastoma cells.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade",
journal = "The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia",
title = "Comparative study of antiproliferative potential of three  different plant species",
pages = "38",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4370"
}

Drakulić, D., Stojković, D., Soković, M.,& Stevanović, M.. (2019). Comparative study of antiproliferative potential of three  different plant species. in The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade., 38.
https://hdl.handle.net/21.15107/rcub_ibiss_4370

Drakulić D, Stojković D, Soković M, Stevanović M. Comparative study of antiproliferative potential of three  different plant species. in The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia. 2019;:38.
https://hdl.handle.net/21.15107/rcub_ibiss_4370 .

Drakulić, Danijela, Stojković, Dejan, Soković, Marina, Stevanović, Milena, "Comparative study of antiproliferative potential of three  different plant species" in The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia (2019):38,
https://hdl.handle.net/21.15107/rcub_ibiss_4370 .

TY  - JOUR
AU  - Drakulić, Danijela D
AU  - Jovicić, Dubravka
AU  - Milacić, Snezana B
AU  - Vukov, Tanja
AU  - Rakić, Boban M
AU  - Stevanović, Milena J
AU  - Rakić, Rada S
AU  - Bukvić, Nenad M
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1381
AB  - We have analyzed the frequency of premature centromeric division (PCD) in medical personnel professionally exposed to low doses of radiation. They had chromosome aberrations (CAs) involving dicentric chromosomes, ring chromosomes, acentric fragments, chromosome breaks, and chromatid breaks. The study included 30 exposed subjects and 23 controls who were each analyzed by a conventional cytogenetics procedure and subsequently by fluorescent in situ hybridization ( FISH). The latter was applied particularly in order to verify PCD in a specific chromosome ( chromosome 18) in both metaphases and interphase nuclei. The results revealed a significant difference (p < 0.001) in frequencies between the two groups ( exposed and controls) for all the observed variables ( CAs), metaphases with PCD (MPCD), total number of chromosomes with PCD (TPCD), number of PCD metaphases in acrocentric chromosomes (MAPCD), and the total number of acrocentric chromosomes with PCD (TAPCD). The doses of ionizing radiation absorbed by the subjects' bodies were measured with thermoluminescent dosimeters once a month during the duration of occupational exposure. They were expressed in mSv, as mean annual effective doses for the period of exposure. The Spearman rank test showed a high positive correlation between total life effective dose and frequency of CAs and PCD. Based on the results obtained in this study, we suggest that PCD, as a phenomenon manifesting chromosomal instability (CIN), should be considered as a suitable cytogenetic biomarker for individuals occupationally exposed to ionizing radiation. Health Phys. 98(5):717-726; 2010
T2  - Health Physics
T1  - Detection of Premature Segregation of Centromeres in Persons Exposed To Ionizing Radiation
IS  - 5
VL  - 98
EP  - 726
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1381
ER  - 

@article{
author = "Drakulić, Danijela D and Jovicić, Dubravka and Milacić, Snezana B and Vukov, Tanja and Rakić, Boban M and Stevanović, Milena J and Rakić, Rada S and Bukvić, Nenad M",
year = "2010",
abstract = "We have analyzed the frequency of premature centromeric division (PCD) in medical personnel professionally exposed to low doses of radiation. They had chromosome aberrations (CAs) involving dicentric chromosomes, ring chromosomes, acentric fragments, chromosome breaks, and chromatid breaks. The study included 30 exposed subjects and 23 controls who were each analyzed by a conventional cytogenetics procedure and subsequently by fluorescent in situ hybridization ( FISH). The latter was applied particularly in order to verify PCD in a specific chromosome ( chromosome 18) in both metaphases and interphase nuclei. The results revealed a significant difference (p < 0.001) in frequencies between the two groups ( exposed and controls) for all the observed variables ( CAs), metaphases with PCD (MPCD), total number of chromosomes with PCD (TPCD), number of PCD metaphases in acrocentric chromosomes (MAPCD), and the total number of acrocentric chromosomes with PCD (TAPCD). The doses of ionizing radiation absorbed by the subjects' bodies were measured with thermoluminescent dosimeters once a month during the duration of occupational exposure. They were expressed in mSv, as mean annual effective doses for the period of exposure. The Spearman rank test showed a high positive correlation between total life effective dose and frequency of CAs and PCD. Based on the results obtained in this study, we suggest that PCD, as a phenomenon manifesting chromosomal instability (CIN), should be considered as a suitable cytogenetic biomarker for individuals occupationally exposed to ionizing radiation. Health Phys. 98(5):717-726; 2010",
journal = "Health Physics",
title = "Detection of Premature Segregation of Centromeres in Persons Exposed To Ionizing Radiation",
number = "5",
volume = "98",
pages = "726",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1381"
}

Drakulić, D. D., Jovicić, D., Milacić, S. B., Vukov, T., Rakić, B. M., Stevanović, M. J., Rakić, R. S.,& Bukvić, N. M.. (2010). Detection of Premature Segregation of Centromeres in Persons Exposed To Ionizing Radiation. in Health Physics, 98(5).
https://hdl.handle.net/21.15107/rcub_ibiss_1381

Drakulić DD, Jovicić D, Milacić SB, Vukov T, Rakić BM, Stevanović MJ, Rakić RS, Bukvić NM. Detection of Premature Segregation of Centromeres in Persons Exposed To Ionizing Radiation. in Health Physics. 2010;98(5):null-726.
https://hdl.handle.net/21.15107/rcub_ibiss_1381 .

Drakulić, Danijela D, Jovicić, Dubravka, Milacić, Snezana B, Vukov, Tanja, Rakić, Boban M, Stevanović, Milena J, Rakić, Rada S, Bukvić, Nenad M, "Detection of Premature Segregation of Centromeres in Persons Exposed To Ionizing Radiation" in Health Physics, 98, no. 5 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1381 .

TY  - JOUR
AU  - Miljković, Đorđe
AU  - Samardzić, Tatjana
AU  - Drakulić, Danijela
AU  - Stošić-Grujičić, Stanislava
AU  - Trajković, Vladimir
PY  - 2002
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6000
AB  - Mycophenolic acid (MPA) and A77 1726, the active components of the immunosuppressants mycophenolate mophetil and leflunomide, respectively, in a dose-dependent manner inhibited interferon (IFN)-gamma/LPS-induced interleukin (IL)-6 release in confluent cultures of mouse L929 fibrosarcoma cells. In addition, both drugs markedly reduced the production of the free radical gas nitric oxide (NO), without affecting the viability of L929 cells. The inhibitors of NO synthase, aminoguanidine and L-NMMA, but not L-NMMA inactive counterpart D-NMMA, mimicked the effects of A77 1726 and MPA on IL-6 generation in L929 fibroblasts. Furthermore, NO-releasing substance SNP completely reverted IL-6 accumulation in L929 cultures treated with A77 1726, while only partial recovery of IL-6 production was observed in the presence of MPA. MPA, but not A77 1726, significantly suppressed NO-independent IL-6 release triggered by cAMP-elevating agent rolipram. Thus, while A77 1726 effect on IL-6 production was mediated through concomitant reduction of NO synthesis, MPA action was mainly independent of the interference with NO generation. Finally, both agents inhibited IFN-gamma/LPS-triggered IL-6 production in mouse primary fibroblasts, but not in mouse peritoneal macrophages, indicating cell-specificity of this novel anti-inflammatory action of A77 1726 and MPA.
PB  - Elsevier
T2  - Cytokine
T1  - Immunosuppressants leflunomide and mycophenolic acid inhibit fibroblast IL-6 production by distinct mechanisms
IS  - 4
VL  - 19
DO  - 10.1006/cyto.2002.0885
SP  - 181
EP  - 186
ER  - 

@article{
author = "Miljković, Đorđe and Samardzić, Tatjana and Drakulić, Danijela and Stošić-Grujičić, Stanislava and Trajković, Vladimir",
year = "2002",
abstract = "Mycophenolic acid (MPA) and A77 1726, the active components of the immunosuppressants mycophenolate mophetil and leflunomide, respectively, in a dose-dependent manner inhibited interferon (IFN)-gamma/LPS-induced interleukin (IL)-6 release in confluent cultures of mouse L929 fibrosarcoma cells. In addition, both drugs markedly reduced the production of the free radical gas nitric oxide (NO), without affecting the viability of L929 cells. The inhibitors of NO synthase, aminoguanidine and L-NMMA, but not L-NMMA inactive counterpart D-NMMA, mimicked the effects of A77 1726 and MPA on IL-6 generation in L929 fibroblasts. Furthermore, NO-releasing substance SNP completely reverted IL-6 accumulation in L929 cultures treated with A77 1726, while only partial recovery of IL-6 production was observed in the presence of MPA. MPA, but not A77 1726, significantly suppressed NO-independent IL-6 release triggered by cAMP-elevating agent rolipram. Thus, while A77 1726 effect on IL-6 production was mediated through concomitant reduction of NO synthesis, MPA action was mainly independent of the interference with NO generation. Finally, both agents inhibited IFN-gamma/LPS-triggered IL-6 production in mouse primary fibroblasts, but not in mouse peritoneal macrophages, indicating cell-specificity of this novel anti-inflammatory action of A77 1726 and MPA.",
publisher = "Elsevier",
journal = "Cytokine",
title = "Immunosuppressants leflunomide and mycophenolic acid inhibit fibroblast IL-6 production by distinct mechanisms",
number = "4",
volume = "19",
doi = "10.1006/cyto.2002.0885",
pages = "181-186"
}

Miljković, Đ., Samardzić, T., Drakulić, D., Stošić-Grujičić, S.,& Trajković, V.. (2002). Immunosuppressants leflunomide and mycophenolic acid inhibit fibroblast IL-6 production by distinct mechanisms. in Cytokine
Elsevier., 19(4), 181-186.
https://doi.org/10.1006/cyto.2002.0885

Miljković Đ, Samardzić T, Drakulić D, Stošić-Grujičić S, Trajković V. Immunosuppressants leflunomide and mycophenolic acid inhibit fibroblast IL-6 production by distinct mechanisms. in Cytokine. 2002;19(4):181-186.
doi:10.1006/cyto.2002.0885 .

Miljković, Đorđe, Samardzić, Tatjana, Drakulić, Danijela, Stošić-Grujičić, Stanislava, Trajković, Vladimir, "Immunosuppressants leflunomide and mycophenolic acid inhibit fibroblast IL-6 production by distinct mechanisms" in Cytokine, 19, no. 4 (2002):181-186,
https://doi.org/10.1006/cyto.2002.0885 . .