TY  - CONF
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Đorđević, Jelena
AU  - Sunjog, Karolina
AU  - Nikolić, Ivan
AU  - Marić, Ana
AU  - Ilić, Marija
AU  - Simonović, Predrag
AU  - Alygizakis, Nikiforos
AU  - Ng, Kelsey
AU  - Oswald, Peter
AU  - Slobodnik, Jaroslav
AU  - Žegura, Bojana
AU  - Vuković-Gačić, Branka
AU  - Paunović, Momir
AU  - Kolarević, Stoimir
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6592
AB  - Assessment of impact of pollution in the environmental studies requires multi-endpoints approach to properly
link cause and effects of pollution with focus on chemical pollution. In this sense systematic weight-of-evidence
approach (WoE) with multiple lines of evidence (LoEs) is preferable to apply. The WoE approach highlights the
importance to identify strengths and weaknesses of used LoEs. Therefore, in this study we have tested efficacy
of genotoxicological endpoints as one of the LoEs in the in situ assessment of pollution effects in the freshwater
ecosystems using Alburnus alburnus (bleak) as a bioindicator species. Additional LoEs that were used in the
study are: component-based methods for the assessment of SumTU in water based on monitoring data of the
Serbian Environmental Protection Agency (SEPA), effect based methods employing in vitro genotoxicological
analyses of Joint Danube Survey 4 (JDS4) water extracts and field derived species inventories for the
assessment and indication of ecological status/potential based on SEPA and JDS4 data. The study was
conducted within the JDS4 campaign at nine sampling sites at the Tisa, Sava, Velika Morava and Danube rivers
in the Republic of Serbia. In the case of three sampling sites, the results were uniform, meaning that all four
LoEs pointed to pollution pressure. The differences in the LoEs outcomes for other sites indicated the
importance of multiple LoEs approach for proper identification of ecological impact. In the case of current
study we have identified comet and micronucleus assay to be appropriate for the genotoxicological assessment
in the in situ studies due to high sensitivity in discrimination of sites in relation to pollution intensity, while
RAPD analysis to be more suitable for controlled ex situ investigations.
PB  - Austrian Committee - International Association for Danube Research
C3  - Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
T1  - In situ genotoxicity assessment in the weight-of-evidence approach – The Joint Danube Survey 4 case study
SP  - 18
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6592
ER  - 

@conference{
author = "Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Đorđević, Jelena and Sunjog, Karolina and Nikolić, Ivan and Marić, Ana and Ilić, Marija and Simonović, Predrag and Alygizakis, Nikiforos and Ng, Kelsey and Oswald, Peter and Slobodnik, Jaroslav and Žegura, Bojana and Vuković-Gačić, Branka and Paunović, Momir and Kolarević, Stoimir",
year = "2023",
abstract = "Assessment of impact of pollution in the environmental studies requires multi-endpoints approach to properly
link cause and effects of pollution with focus on chemical pollution. In this sense systematic weight-of-evidence
approach (WoE) with multiple lines of evidence (LoEs) is preferable to apply. The WoE approach highlights the
importance to identify strengths and weaknesses of used LoEs. Therefore, in this study we have tested efficacy
of genotoxicological endpoints as one of the LoEs in the in situ assessment of pollution effects in the freshwater
ecosystems using Alburnus alburnus (bleak) as a bioindicator species. Additional LoEs that were used in the
study are: component-based methods for the assessment of SumTU in water based on monitoring data of the
Serbian Environmental Protection Agency (SEPA), effect based methods employing in vitro genotoxicological
analyses of Joint Danube Survey 4 (JDS4) water extracts and field derived species inventories for the
assessment and indication of ecological status/potential based on SEPA and JDS4 data. The study was
conducted within the JDS4 campaign at nine sampling sites at the Tisa, Sava, Velika Morava and Danube rivers
in the Republic of Serbia. In the case of three sampling sites, the results were uniform, meaning that all four
LoEs pointed to pollution pressure. The differences in the LoEs outcomes for other sites indicated the
importance of multiple LoEs approach for proper identification of ecological impact. In the case of current
study we have identified comet and micronucleus assay to be appropriate for the genotoxicological assessment
in the in situ studies due to high sensitivity in discrimination of sites in relation to pollution intensity, while
RAPD analysis to be more suitable for controlled ex situ investigations.",
publisher = "Austrian Committee - International Association for Danube Research",
journal = "Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria",
title = "In situ genotoxicity assessment in the weight-of-evidence approach – The Joint Danube Survey 4 case study",
pages = "18",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6592"
}

Kračun-Kolarević, M., Jovanović Marić, J., Đorđević, J., Sunjog, K., Nikolić, I., Marić, A., Ilić, M., Simonović, P., Alygizakis, N., Ng, K., Oswald, P., Slobodnik, J., Žegura, B., Vuković-Gačić, B., Paunović, M.,& Kolarević, S.. (2023). In situ genotoxicity assessment in the weight-of-evidence approach – The Joint Danube Survey 4 case study. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
Austrian Committee - International Association for Danube Research., 18.
https://hdl.handle.net/21.15107/rcub_ibiss_6592

Kračun-Kolarević M, Jovanović Marić J, Đorđević J, Sunjog K, Nikolić I, Marić A, Ilić M, Simonović P, Alygizakis N, Ng K, Oswald P, Slobodnik J, Žegura B, Vuković-Gačić B, Paunović M, Kolarević S. In situ genotoxicity assessment in the weight-of-evidence approach – The Joint Danube Survey 4 case study. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria. 2023;:18.
https://hdl.handle.net/21.15107/rcub_ibiss_6592 .

Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Đorđević, Jelena, Sunjog, Karolina, Nikolić, Ivan, Marić, Ana, Ilić, Marija, Simonović, Predrag, Alygizakis, Nikiforos, Ng, Kelsey, Oswald, Peter, Slobodnik, Jaroslav, Žegura, Bojana, Vuković-Gačić, Branka, Paunović, Momir, Kolarević, Stoimir, "In situ genotoxicity assessment in the weight-of-evidence approach – The Joint Danube Survey 4 case study" in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria (2023):18,
https://hdl.handle.net/21.15107/rcub_ibiss_6592 .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Kračun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Đorđević, Jelena
AU  - Sunjog, Karolina
AU  - Kostić-Vuković, Jovana
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5724
AB  - The middle section of the Danube River basin in the previous three Joint
Danube Surveys (JDS) was found to be under intensive pollution, mainly
affected by wastewaters. As a consequence of the lack of wastewater
treatment plants, complex xenobiotics mixtures may get into surface water
and decrease water quality and organisms’ health. Some xenobiotics may
induce different types of DNA damage. Hence, DNA damage could be a
useful biomarker in the detection of “early warning signals” of organisms’
exposure to genotoxic compounds, while the usage of bioassays batteries
can provide better insight into a genotoxic potential. This study was
conducted in 2019, during the JDS4 expedition, to assess the genotoxic
potential of nine sites on the section of the Danube River which goes
through Serbia and its major tributaries: the Sava, Tisza and Velika Morava.
Blood and muscle of Alburnus alburnus (bleak) were used for genotoxicity
assessment by comet, micronucleus and RAPD assays. In comet assay, the
highest level of DNA damage was recorded at the Velika Morava mouth (JDS
39) and on the Danube River, downstream Radujevac (JDS 41). The highest
frequency of micronucleus was recorded at the Sava mouth (JDS 36) and on
the Danube River, downstream Pančevo (JDS 37). The lowest level of DNA
damage in both tests was recorded on the Sava, site Jamena (JDS 35). The
RAPD analysis distinct three major groups: Tisza mouth (JDS 33) and sites
on the Sava (JDS 35, JDS 36), then two sites on the Danube River (JDS 37,
JDS 41) and one site on the Danube River, Ram (JDS 40) with the Velika
Morava mouth (JDS 39). Analyzed bioassays showed different sensitivity
where the comet assay had the highest potential in discrimination of sites
based on genotoxicity. Bleak was proved to be a reliable bioindicator in eco/
genotoxicological studies.
PB  - European Environmental Mutagenesis & Genomics Society
C3  - Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands
T1  - Evaluation of genotoxic potential of the middle section of the Danube River and its major tributaries.
SP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5724
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Kračun-Kolarević, Margareta and Kolarević, Stoimir and Đorđević, Jelena and Sunjog, Karolina and Kostić-Vuković, Jovana and Paunović, Momir and Vuković-Gačić, Branka",
year = "2022",
abstract = "The middle section of the Danube River basin in the previous three Joint
Danube Surveys (JDS) was found to be under intensive pollution, mainly
affected by wastewaters. As a consequence of the lack of wastewater
treatment plants, complex xenobiotics mixtures may get into surface water
and decrease water quality and organisms’ health. Some xenobiotics may
induce different types of DNA damage. Hence, DNA damage could be a
useful biomarker in the detection of “early warning signals” of organisms’
exposure to genotoxic compounds, while the usage of bioassays batteries
can provide better insight into a genotoxic potential. This study was
conducted in 2019, during the JDS4 expedition, to assess the genotoxic
potential of nine sites on the section of the Danube River which goes
through Serbia and its major tributaries: the Sava, Tisza and Velika Morava.
Blood and muscle of Alburnus alburnus (bleak) were used for genotoxicity
assessment by comet, micronucleus and RAPD assays. In comet assay, the
highest level of DNA damage was recorded at the Velika Morava mouth (JDS
39) and on the Danube River, downstream Radujevac (JDS 41). The highest
frequency of micronucleus was recorded at the Sava mouth (JDS 36) and on
the Danube River, downstream Pančevo (JDS 37). The lowest level of DNA
damage in both tests was recorded on the Sava, site Jamena (JDS 35). The
RAPD analysis distinct three major groups: Tisza mouth (JDS 33) and sites
on the Sava (JDS 35, JDS 36), then two sites on the Danube River (JDS 37,
JDS 41) and one site on the Danube River, Ram (JDS 40) with the Velika
Morava mouth (JDS 39). Analyzed bioassays showed different sensitivity
where the comet assay had the highest potential in discrimination of sites
based on genotoxicity. Bleak was proved to be a reliable bioindicator in eco/
genotoxicological studies.",
publisher = "European Environmental Mutagenesis & Genomics Society",
journal = "Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands",
title = "Evaluation of genotoxic potential of the middle section of the Danube River and its major tributaries.",
pages = "69",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5724"
}

Jovanović Marić, J., Kračun-Kolarević, M., Kolarević, S., Đorđević, J., Sunjog, K., Kostić-Vuković, J., Paunović, M.,& Vuković-Gačić, B.. (2022). Evaluation of genotoxic potential of the middle section of the Danube River and its major tributaries.. in Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands
European Environmental Mutagenesis & Genomics Society., 69.
https://hdl.handle.net/21.15107/rcub_ibiss_5724

Jovanović Marić J, Kračun-Kolarević M, Kolarević S, Đorđević J, Sunjog K, Kostić-Vuković J, Paunović M, Vuković-Gačić B. Evaluation of genotoxic potential of the middle section of the Danube River and its major tributaries.. in Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands. 2022;:69.
https://hdl.handle.net/21.15107/rcub_ibiss_5724 .

Jovanović Marić, Jovana, Kračun-Kolarević, Margareta, Kolarević, Stoimir, Đorđević, Jelena, Sunjog, Karolina, Kostić-Vuković, Jovana, Paunović, Momir, Vuković-Gačić, Branka, "Evaluation of genotoxic potential of the middle section of the Danube River and its major tributaries." in Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands (2022):69,
https://hdl.handle.net/21.15107/rcub_ibiss_5724 .

TY  - CONF
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Đorđević, Jelena
AU  - Paunović, Momir
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Gačić, Zoran
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5722
AB  - Within the past decade, our research group placed great effort in
exploration of the impact of treated/untreated wastewaters on aquatic
ecosystems as less than 13 % of collected municipal wastewaters are
processed before their release to receiving waters in Serbia. Having in mind
that more than 90% of the rivers within the territory of Serbia belong to the
Danube River Basin, our major focus is naturally on the large transnational
waters such as the Danube and Sava rivers. Depended on the concept of the
study and hydro-morphological characteristics of the sites, various
approaches have been employed for in situ assessment of eco/geno-toxicity
which use different organisms in passive and active biomonitoring. Aquatic
organisms of different trophic levels have diverse life strategies, metabolism
pathways, and consequently, they have a different response to pollutant
pressure. In eco/geno-toxicological studies we have successfully employed
several species of freshwater mussels (Unio tumidus, Unio pictorum and
Sinanodonta woodiana) as well as few economically important fish species
(common carp (Cyprinus carpio), freshwater bream (Abramis brama), bleak
(Alburnus alburnus, chub (Squalius cephalus)).Comet assay is our primary
choice for the assessment of DNA damage considering its sensitivity and
cost effectiveness. Selection of certain types of tissues for the assessment
(such as blood or haemolymph), which do not require sacrificing of the
animals or additional manipulation in preparation of cell suspension coupled
with mini-gel format of the slides enables high-throughput screening of the
genotoxic potential in situ. Additionally, cryopreservation of the blood
samples directly onsite expands the research area as it overcomes issues
related to safe transportation of the samples to the laboratory. Data
obtained so far indicated that the genotoxic response in the studied
indicator organisms significantly differ which is understandable considering
the difference in uptake, accumulation and physiological responses.
PB  - European Environmental Mutagenesis & Genomics Society
C3  - Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands
T1  - Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research.
SP  - 42
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5722
ER  - 

@conference{
author = "Kolarević, Stoimir and Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Đorđević, Jelena and Paunović, Momir and Kostić-Vuković, Jovana and Sunjog, Karolina and Gačić, Zoran and Vuković-Gačić, Branka",
year = "2022",
abstract = "Within the past decade, our research group placed great effort in
exploration of the impact of treated/untreated wastewaters on aquatic
ecosystems as less than 13 % of collected municipal wastewaters are
processed before their release to receiving waters in Serbia. Having in mind
that more than 90% of the rivers within the territory of Serbia belong to the
Danube River Basin, our major focus is naturally on the large transnational
waters such as the Danube and Sava rivers. Depended on the concept of the
study and hydro-morphological characteristics of the sites, various
approaches have been employed for in situ assessment of eco/geno-toxicity
which use different organisms in passive and active biomonitoring. Aquatic
organisms of different trophic levels have diverse life strategies, metabolism
pathways, and consequently, they have a different response to pollutant
pressure. In eco/geno-toxicological studies we have successfully employed
several species of freshwater mussels (Unio tumidus, Unio pictorum and
Sinanodonta woodiana) as well as few economically important fish species
(common carp (Cyprinus carpio), freshwater bream (Abramis brama), bleak
(Alburnus alburnus, chub (Squalius cephalus)).Comet assay is our primary
choice for the assessment of DNA damage considering its sensitivity and
cost effectiveness. Selection of certain types of tissues for the assessment
(such as blood or haemolymph), which do not require sacrificing of the
animals or additional manipulation in preparation of cell suspension coupled
with mini-gel format of the slides enables high-throughput screening of the
genotoxic potential in situ. Additionally, cryopreservation of the blood
samples directly onsite expands the research area as it overcomes issues
related to safe transportation of the samples to the laboratory. Data
obtained so far indicated that the genotoxic response in the studied
indicator organisms significantly differ which is understandable considering
the difference in uptake, accumulation and physiological responses.",
publisher = "European Environmental Mutagenesis & Genomics Society",
journal = "Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands",
title = "Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research.",
pages = "42",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5722"
}

Kolarević, S., Kračun-Kolarević, M., Jovanović Marić, J., Đorđević, J., Paunović, M., Kostić-Vuković, J., Sunjog, K., Gačić, Z.,& Vuković-Gačić, B.. (2022). Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research.. in Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands
European Environmental Mutagenesis & Genomics Society., 42.
https://hdl.handle.net/21.15107/rcub_ibiss_5722

Kolarević S, Kračun-Kolarević M, Jovanović Marić J, Đorđević J, Paunović M, Kostić-Vuković J, Sunjog K, Gačić Z, Vuković-Gačić B. Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research.. in Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands. 2022;:42.
https://hdl.handle.net/21.15107/rcub_ibiss_5722 .

Kolarević, Stoimir, Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Đorđević, Jelena, Paunović, Momir, Kostić-Vuković, Jovana, Sunjog, Karolina, Gačić, Zoran, Vuković-Gačić, Branka, "Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research." in Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands (2022):42,
https://hdl.handle.net/21.15107/rcub_ibiss_5722 .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Kračun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Đorđević, Jelena
AU  - Sunjog, Karolina
AU  - Kostić-Vuković, Jovana
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4937
AB  - Velika, Zapadna, Južna Morava, kao i Sava, Kolubara i Barička reka su pod značajnim uticajem komunalnih i industrijskih otpadnih voda. Ciljevi studije su procena genotoksičnog potencijala analiziranih lokaliteta i ispitivanje osetljivosti primenjenih biotestova i pristupa istraživanja. Studija slučaja Velika Morava obuhvatila je veliko ispitivano područje na osam lokaliteta na Velikoj, Zapadnoj i Južnoj Moravi, a u studiji Sava ispitivano je tri prostorno bliska lokaliteta koji su pod različitim pritiscima zagađenja. U in silico pristupu na osnovu literaturnih podataka analiziran je toksični/genotoksični potencijal jedinjenja detektovanih u vodi. Ex situ/in vitro pristup obuhvatio je ispitivanje genotoksičnog potencijala pomoću SOS/umuC testa, kao i primenom alkalnog komet testa na HepG2 ćelijskoj liniji. U in situ/in vivo istraživanju analizirano je DNK oštećenje ćelija krvi vrste Alburnus alburnus (uklija) pomoću alkalnog, Fpg-modifikovanog komet testa i mikronukleus testa. Rezultati su pokazali različitu osetljivost zavisno od upotrebljenih testova i pristupa, ali i različit odgovor kod uklija u zavisnosti od tipa zagađenja na lokalitetima. In silico i ex situ/in vitro pristupi su pokazali niži stepen osetljivosti u odnosu na in situ/in vivo testove, a alkalni komet test je pokazao najveći potencijal u diskriminaciji lokaliteta. Utvrđeno je da uklija može biti pouzdan bioindikator u ekogenotoksikološkim istraživanjima velikih ravničarskih reka.
PB  - Novi Sad : Prirodno-matematički fakultet
C3  - 9. memorijalni naučni skup iz zaštite životne sredine "Docent dr Milena Dalmacija": 2022 Mar 31 - Apr 1; Novi Sad, Serbia
T1  - Ispitivanje osetljivosti testova i pristupa u ekogenotoksikološkim istraživanjima na velikim ravničarskim rekama – studije slučaja Velika Morava i Sava
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4937
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Kračun-Kolarević, Margareta and Kolarević, Stoimir and Đorđević, Jelena and Sunjog, Karolina and Kostić-Vuković, Jovana and Paunović, Momir and Vuković-Gačić, Branka",
year = "2022",
abstract = "Velika, Zapadna, Južna Morava, kao i Sava, Kolubara i Barička reka su pod značajnim uticajem komunalnih i industrijskih otpadnih voda. Ciljevi studije su procena genotoksičnog potencijala analiziranih lokaliteta i ispitivanje osetljivosti primenjenih biotestova i pristupa istraživanja. Studija slučaja Velika Morava obuhvatila je veliko ispitivano područje na osam lokaliteta na Velikoj, Zapadnoj i Južnoj Moravi, a u studiji Sava ispitivano je tri prostorno bliska lokaliteta koji su pod različitim pritiscima zagađenja. U in silico pristupu na osnovu literaturnih podataka analiziran je toksični/genotoksični potencijal jedinjenja detektovanih u vodi. Ex situ/in vitro pristup obuhvatio je ispitivanje genotoksičnog potencijala pomoću SOS/umuC testa, kao i primenom alkalnog komet testa na HepG2 ćelijskoj liniji. U in situ/in vivo istraživanju analizirano je DNK oštećenje ćelija krvi vrste Alburnus alburnus (uklija) pomoću alkalnog, Fpg-modifikovanog komet testa i mikronukleus testa. Rezultati su pokazali različitu osetljivost zavisno od upotrebljenih testova i pristupa, ali i različit odgovor kod uklija u zavisnosti od tipa zagađenja na lokalitetima. In silico i ex situ/in vitro pristupi su pokazali niži stepen osetljivosti u odnosu na in situ/in vivo testove, a alkalni komet test je pokazao najveći potencijal u diskriminaciji lokaliteta. Utvrđeno je da uklija može biti pouzdan bioindikator u ekogenotoksikološkim istraživanjima velikih ravničarskih reka.",
publisher = "Novi Sad : Prirodno-matematički fakultet",
journal = "9. memorijalni naučni skup iz zaštite životne sredine "Docent dr Milena Dalmacija": 2022 Mar 31 - Apr 1; Novi Sad, Serbia",
title = "Ispitivanje osetljivosti testova i pristupa u ekogenotoksikološkim istraživanjima na velikim ravničarskim rekama – studije slučaja Velika Morava i Sava",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4937"
}

Jovanović Marić, J., Kračun-Kolarević, M., Kolarević, S., Đorđević, J., Sunjog, K., Kostić-Vuković, J., Paunović, M.,& Vuković-Gačić, B.. (2022). Ispitivanje osetljivosti testova i pristupa u ekogenotoksikološkim istraživanjima na velikim ravničarskim rekama – studije slučaja Velika Morava i Sava. in 9. memorijalni naučni skup iz zaštite životne sredine "Docent dr Milena Dalmacija": 2022 Mar 31 - Apr 1; Novi Sad, Serbia
Novi Sad : Prirodno-matematički fakultet..
https://hdl.handle.net/21.15107/rcub_ibiss_4937

Jovanović Marić J, Kračun-Kolarević M, Kolarević S, Đorđević J, Sunjog K, Kostić-Vuković J, Paunović M, Vuković-Gačić B. Ispitivanje osetljivosti testova i pristupa u ekogenotoksikološkim istraživanjima na velikim ravničarskim rekama – studije slučaja Velika Morava i Sava. in 9. memorijalni naučni skup iz zaštite životne sredine "Docent dr Milena Dalmacija": 2022 Mar 31 - Apr 1; Novi Sad, Serbia. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_4937 .

Jovanović Marić, Jovana, Kračun-Kolarević, Margareta, Kolarević, Stoimir, Đorđević, Jelena, Sunjog, Karolina, Kostić-Vuković, Jovana, Paunović, Momir, Vuković-Gačić, Branka, "Ispitivanje osetljivosti testova i pristupa u ekogenotoksikološkim istraživanjima na velikim ravničarskim rekama – studije slučaja Velika Morava i Sava" in 9. memorijalni naučni skup iz zaštite životne sredine "Docent dr Milena Dalmacija": 2022 Mar 31 - Apr 1; Novi Sad, Serbia (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_4937 .

TY  - CONF
AU  - Đorđević, Jelena
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Kolarević, Stoimir
AU  - Višnjić-Jeftić, Željka
AU  - Subotić, Srđan
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4729
AB  - Water quality in the Danube River Basin is largely influenced by the inputs of pollutants - particularly
excessive nutrients, organic material, and hazardous substances, which affects the whole ecosystem
at various levels. The major goal of this study was to evaluate the level of genotoxic potential at the
site at Danube River, Novi Banovci, which is under significant anthropogenic pressure of untreated
municipal and industrial waste waters.
DNA damage in situ was assessed in 5 specimens of white bream (Blicca bjoerkna) erythrocytes, liver
and gills cells with comet assay and in erythrocytes with micronucleus test. Additionally, genotoxicity
of native water samples collected from the site and upstream and downstream of the site was
evaluated by the comet assay on HepG2 cells. Microscopic images of comets were scored using Comet
IV Computer Software (Perceptive Instruments, UK) and tail intensity was chosen as parameters to
assess the DNA damage.
Comet assay on white bream showed that gills were the most affected tissue, while liver showed the
smallest values. Micronuclei frequency was low in general, with mean of 1 micronuclei in 5000 scored
erythrocytes, with no significant correlation to the values of comet assay in erythrocytes. Comet assay
on HepG2 cells showed significant difference between values of negative control samples and samples
of water from effluent site and downstream of wastewater discharge. There was no significant
difference between negative control and upstream samples of water.
Our results showed that great impact of municipal and industrial wastewaters on the river ecosystem.
The application of in vitro and in situ tests gives a better insight into genotoxic effects. More
importantly, because Serbia still doesn’t have waste water treatme nt plants, this kind of research
points out the imperative for implementation of this facilities.
PB  - Neuburg:  Aueninstitut Neuburg
C3  - 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin
T1  - Genotoxicity assessment of Danube River: in situ and in vitro methods
SP  - 18
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4729
ER  - 

@conference{
author = "Đorđević, Jelena and Kostić-Vuković, Jovana and Sunjog, Karolina and Kolarević, Stoimir and Višnjić-Jeftić, Željka and Subotić, Srđan and Gačić, Zoran and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2021",
abstract = "Water quality in the Danube River Basin is largely influenced by the inputs of pollutants - particularly
excessive nutrients, organic material, and hazardous substances, which affects the whole ecosystem
at various levels. The major goal of this study was to evaluate the level of genotoxic potential at the
site at Danube River, Novi Banovci, which is under significant anthropogenic pressure of untreated
municipal and industrial waste waters.
DNA damage in situ was assessed in 5 specimens of white bream (Blicca bjoerkna) erythrocytes, liver
and gills cells with comet assay and in erythrocytes with micronucleus test. Additionally, genotoxicity
of native water samples collected from the site and upstream and downstream of the site was
evaluated by the comet assay on HepG2 cells. Microscopic images of comets were scored using Comet
IV Computer Software (Perceptive Instruments, UK) and tail intensity was chosen as parameters to
assess the DNA damage.
Comet assay on white bream showed that gills were the most affected tissue, while liver showed the
smallest values. Micronuclei frequency was low in general, with mean of 1 micronuclei in 5000 scored
erythrocytes, with no significant correlation to the values of comet assay in erythrocytes. Comet assay
on HepG2 cells showed significant difference between values of negative control samples and samples
of water from effluent site and downstream of wastewater discharge. There was no significant
difference between negative control and upstream samples of water.
Our results showed that great impact of municipal and industrial wastewaters on the river ecosystem.
The application of in vitro and in situ tests gives a better insight into genotoxic effects. More
importantly, because Serbia still doesn’t have waste water treatme nt plants, this kind of research
points out the imperative for implementation of this facilities.",
publisher = "Neuburg:  Aueninstitut Neuburg",
journal = "43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin",
title = "Genotoxicity assessment of Danube River: in situ and in vitro methods",
pages = "18",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4729"
}

Đorđević, J., Kostić-Vuković, J., Sunjog, K., Kolarević, S., Višnjić-Jeftić, Ž., Subotić, S., Gačić, Z., Lenhardt, M.,& Vuković-Gačić, B.. (2021). Genotoxicity assessment of Danube River: in situ and in vitro methods. in 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin
Neuburg:  Aueninstitut Neuburg., 18.
https://hdl.handle.net/21.15107/rcub_ibiss_4729

Đorđević J, Kostić-Vuković J, Sunjog K, Kolarević S, Višnjić-Jeftić Ž, Subotić S, Gačić Z, Lenhardt M, Vuković-Gačić B. Genotoxicity assessment of Danube River: in situ and in vitro methods. in 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin. 2021;:18.
https://hdl.handle.net/21.15107/rcub_ibiss_4729 .

Đorđević, Jelena, Kostić-Vuković, Jovana, Sunjog, Karolina, Kolarević, Stoimir, Višnjić-Jeftić, Željka, Subotić, Srđan, Gačić, Zoran, Lenhardt, Mirjana, Vuković-Gačić, Branka, "Genotoxicity assessment of Danube River: in situ and in vitro methods" in 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin (2021):18,
https://hdl.handle.net/21.15107/rcub_ibiss_4729 .

TY  - JOUR
AU  - Sunjog, Karolina
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Višnjić-Jeftić, Željka
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2019
UR  - http://link.springer.com/10.1007/s11356-019-04274-3
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3267
AB  - Due to the increasing industrialization, metals are discharged into all spheres of the environment, in particular, in river courses, which leads to the need for constant monitoring. Metals do not degrade into harmless end products; they are very persistent and have high potential for accumulation in biota. Metals in the fish body are accumulated in different amounts in the tissue specific matter. In relation to the biotic conditions and fish metabolism, the load of food, or the stage of the reproductive cycle, the seasonal variation of metal levels can be expected. Because of that, the objective of our present study was to analyze 15 metals and metalloids in liver, gills, muscle, and gonads of European chub (Squalius cephalus) throughout the 4 seasons, autumn, winter, spring, and summer. The specimens were collected from two rivers, Pestan and Beljanica at the Kolubara basin, and their concentrations were determined with inductively coupled plasma optical emission spectrometry (ICP-OES). Specimens from both rivers have shown similarities in metal accumulation like the highest accumulation of majority of elements in gills, lowest accumulation of majority of elements in muscle (except for Hg), and higher accumulation of some elements in summer (Cu, Fe, Zn). In addition, Cu and Fe showed affinity for liver, while Ba, Cr, Sr, and Zn were specific for gills. Also, Al, B, Fe, Ni, and Pb did not show significant differences in concentrations among different seasons in all investigated tissues.
T2  - Environmental Science and Pollution Research
T1  - Seasonal variation in metal concentration in various tissues of the European chub (Squalius cephalus L.).
DO  - 10.1007/s11356-019-04274-3
ER  - 

@article{
author = "Sunjog, Karolina and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Višnjić-Jeftić, Željka and Gačić, Zoran and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2019",
abstract = "Due to the increasing industrialization, metals are discharged into all spheres of the environment, in particular, in river courses, which leads to the need for constant monitoring. Metals do not degrade into harmless end products; they are very persistent and have high potential for accumulation in biota. Metals in the fish body are accumulated in different amounts in the tissue specific matter. In relation to the biotic conditions and fish metabolism, the load of food, or the stage of the reproductive cycle, the seasonal variation of metal levels can be expected. Because of that, the objective of our present study was to analyze 15 metals and metalloids in liver, gills, muscle, and gonads of European chub (Squalius cephalus) throughout the 4 seasons, autumn, winter, spring, and summer. The specimens were collected from two rivers, Pestan and Beljanica at the Kolubara basin, and their concentrations were determined with inductively coupled plasma optical emission spectrometry (ICP-OES). Specimens from both rivers have shown similarities in metal accumulation like the highest accumulation of majority of elements in gills, lowest accumulation of majority of elements in muscle (except for Hg), and higher accumulation of some elements in summer (Cu, Fe, Zn). In addition, Cu and Fe showed affinity for liver, while Ba, Cr, Sr, and Zn were specific for gills. Also, Al, B, Fe, Ni, and Pb did not show significant differences in concentrations among different seasons in all investigated tissues.",
journal = "Environmental Science and Pollution Research",
title = "Seasonal variation in metal concentration in various tissues of the European chub (Squalius cephalus L.).",
doi = "10.1007/s11356-019-04274-3"
}

Sunjog, K., Kolarević, S., Kračun-Kolarević, M., Višnjić-Jeftić, Ž., Gačić, Z., Lenhardt, M.,& Vuković-Gačić, B.. (2019). Seasonal variation in metal concentration in various tissues of the European chub (Squalius cephalus L.).. in Environmental Science and Pollution Research.
https://doi.org/10.1007/s11356-019-04274-3

Sunjog K, Kolarević S, Kračun-Kolarević M, Višnjić-Jeftić Ž, Gačić Z, Lenhardt M, Vuković-Gačić B. Seasonal variation in metal concentration in various tissues of the European chub (Squalius cephalus L.).. in Environmental Science and Pollution Research. 2019;.
doi:10.1007/s11356-019-04274-3 .

Sunjog, Karolina, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Višnjić-Jeftić, Željka, Gačić, Zoran, Lenhardt, Mirjana, Vuković-Gačić, Branka, "Seasonal variation in metal concentration in various tissues of the European chub (Squalius cephalus L.)." in Environmental Science and Pollution Research (2019),
https://doi.org/10.1007/s11356-019-04274-3 . .

TY  - JOUR
AU  - Jovanović, Jovana
AU  - Kolarević, Stoimir
AU  - Milošković, Aleksandra
AU  - Radojković, Nataša
AU  - Simić, Vladica
AU  - Dojčinović, Biljana
AU  - Kračun-Kolarević, Margareta
AU  - Paunović, Momir
AU  - Kostić, Jovana
AU  - Sunjog, Karolina
AU  - Gačić, Zoran
AU  - Vuković-Gačić, Branka
AU  - Timilijić, Jovana
AU  - Djordjević, Jelena
AU  - Žegura, Bojana
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S004896971732805X
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2887
AB  - The Velika Morava River is the greatest national Serbian river and the significant tributary of the Danube River. The major problems in the Velika Morava River Basin (VMRB) represent untreated industrial and municipal wastewaters. In this study, the level of genotoxic potential at the sites along the VMRB was evaluated by parallel in vitro and in situ approach. Within in vitro testing, genotoxicity of native water samples collected from the sites in VMRB was evaluated by SOS/umuC test on Salmonella typhimurium TA1535/pSK1002 and by the comet assay on HepG2 cells. DNA damage in situ was assessed in bleak (Alburnus alburnus) erythrocytes by the comet (alkaline and Fpg-modified comet) and micronucleus assays. Additionally, the concentration of heavy metals in fish tissue was measured and this data, compiled with the data of the physico-chemical parameters measured in water, was used as a measure of the pollution pressure at the sites. Results showed that applied in vitro tests with native water samples are less sensitive in comparison with in situ tests and should be taken with precaution when making predictions on the status of the ecosystem. Within applied battery of in situ assays differential sensitivity of assays was observed where alkaline comet assay showed the highest potential in differentiation of the sites based on genotoxic potential. Integrated biomarker response showed that usage of the battery of bioassays provides better insight in a genotoxic effects in animals, and consequently, that the holistic approach is more suitable for this type of study.
T2  - Science of The Total Environment
T1  - Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ
VL  - 621
DO  - 10.1016/j.scitotenv.2017.10.099
SP  - 1289
EP  - 1299
ER  - 

@article{
author = "Jovanović, Jovana and Kolarević, Stoimir and Milošković, Aleksandra and Radojković, Nataša and Simić, Vladica and Dojčinović, Biljana and Kračun-Kolarević, Margareta and Paunović, Momir and Kostić, Jovana and Sunjog, Karolina and Gačić, Zoran and Vuković-Gačić, Branka and Timilijić, Jovana and Djordjević, Jelena and Žegura, Bojana",
year = "2018",
abstract = "The Velika Morava River is the greatest national Serbian river and the significant tributary of the Danube River. The major problems in the Velika Morava River Basin (VMRB) represent untreated industrial and municipal wastewaters. In this study, the level of genotoxic potential at the sites along the VMRB was evaluated by parallel in vitro and in situ approach. Within in vitro testing, genotoxicity of native water samples collected from the sites in VMRB was evaluated by SOS/umuC test on Salmonella typhimurium TA1535/pSK1002 and by the comet assay on HepG2 cells. DNA damage in situ was assessed in bleak (Alburnus alburnus) erythrocytes by the comet (alkaline and Fpg-modified comet) and micronucleus assays. Additionally, the concentration of heavy metals in fish tissue was measured and this data, compiled with the data of the physico-chemical parameters measured in water, was used as a measure of the pollution pressure at the sites. Results showed that applied in vitro tests with native water samples are less sensitive in comparison with in situ tests and should be taken with precaution when making predictions on the status of the ecosystem. Within applied battery of in situ assays differential sensitivity of assays was observed where alkaline comet assay showed the highest potential in differentiation of the sites based on genotoxic potential. Integrated biomarker response showed that usage of the battery of bioassays provides better insight in a genotoxic effects in animals, and consequently, that the holistic approach is more suitable for this type of study.",
journal = "Science of The Total Environment",
title = "Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ",
volume = "621",
doi = "10.1016/j.scitotenv.2017.10.099",
pages = "1289-1299"
}

Jovanović, J., Kolarević, S., Milošković, A., Radojković, N., Simić, V., Dojčinović, B., Kračun-Kolarević, M., Paunović, M., Kostić, J., Sunjog, K., Gačić, Z., Vuković-Gačić, B., Timilijić, J., Djordjević, J.,& Žegura, B.. (2018). Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ. in Science of The Total Environment, 621, 1289-1299.
https://doi.org/10.1016/j.scitotenv.2017.10.099

Jovanović J, Kolarević S, Milošković A, Radojković N, Simić V, Dojčinović B, Kračun-Kolarević M, Paunović M, Kostić J, Sunjog K, Gačić Z, Vuković-Gačić B, Timilijić J, Djordjević J, Žegura B. Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ. in Science of The Total Environment. 2018;621:1289-1299.
doi:10.1016/j.scitotenv.2017.10.099 .

Jovanović, Jovana, Kolarević, Stoimir, Milošković, Aleksandra, Radojković, Nataša, Simić, Vladica, Dojčinović, Biljana, Kračun-Kolarević, Margareta, Paunović, Momir, Kostić, Jovana, Sunjog, Karolina, Gačić, Zoran, Vuković-Gačić, Branka, Timilijić, Jovana, Djordjević, Jelena, Žegura, Bojana, "Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ" in Science of The Total Environment, 621 (2018):1289-1299,
https://doi.org/10.1016/j.scitotenv.2017.10.099 . .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Kračun-Kolarević, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Jovanović, Jovana
AU  - Simonović, Predrag
AU  - Simić, Vladica
AU  - Piria, Marina
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2018
UR  - http://link.springer.com/10.1007/s41742-018-0127-6
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3117
AB  - The major objective of this study was to investigate the impact of wastewaters on the level of genotoxicity and the possible reflection of genotoxicity on the genetic variability in chub (Squalius cephalus) collected from the sites under different pollution pressures along the Sava River. To emphasize the impact of wastewaters, this study was carried out in a season with low water discharge. The level of genotoxic potential at the sites was studied by alkaline and Fpg-modified comet assay in fish blood, while RAPD technique was employed on the fish muscle tissue. The variations in the genotoxic potential were observed along the Sava River based on the level of DNA damage in blood of the specimens collected at the selected sites. Clustering of the impacted sites was also observed based on RAPD fingerprints. Comparison of the data obtained in RAPD and the data from the comet assay indicated the possible reflection of genotoxicity on the genetic variability in chub collected from the sites with different pollution pressure. This is a preliminary study which reports the differentiation of the population of chub along the Sava River based on RAPD analysis.
T2  - International Journal of Environmental Research
T1  - The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study
DO  - 10.1007/s41742-018-0127-6
ER  - 

@article{
author = "Kolarević, Stoimir and Sunjog, Karolina and Kračun-Kolarević, Margareta and Kostić-Vuković, Jovana and Jovanović, Jovana and Simonović, Predrag and Simić, Vladica and Piria, Marina and Gačić, Zoran and Lenhardt, Mirjana and Paunović, Momir and Vuković-Gačić, Branka",
year = "2018",
abstract = "The major objective of this study was to investigate the impact of wastewaters on the level of genotoxicity and the possible reflection of genotoxicity on the genetic variability in chub (Squalius cephalus) collected from the sites under different pollution pressures along the Sava River. To emphasize the impact of wastewaters, this study was carried out in a season with low water discharge. The level of genotoxic potential at the sites was studied by alkaline and Fpg-modified comet assay in fish blood, while RAPD technique was employed on the fish muscle tissue. The variations in the genotoxic potential were observed along the Sava River based on the level of DNA damage in blood of the specimens collected at the selected sites. Clustering of the impacted sites was also observed based on RAPD fingerprints. Comparison of the data obtained in RAPD and the data from the comet assay indicated the possible reflection of genotoxicity on the genetic variability in chub collected from the sites with different pollution pressure. This is a preliminary study which reports the differentiation of the population of chub along the Sava River based on RAPD analysis.",
journal = "International Journal of Environmental Research",
title = "The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study",
doi = "10.1007/s41742-018-0127-6"
}

Kolarević, S., Sunjog, K., Kračun-Kolarević, M., Kostić-Vuković, J., Jovanović, J., Simonović, P., Simić, V., Piria, M., Gačić, Z., Lenhardt, M., Paunović, M.,& Vuković-Gačić, B.. (2018). The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study. in International Journal of Environmental Research.
https://doi.org/10.1007/s41742-018-0127-6

Kolarević S, Sunjog K, Kračun-Kolarević M, Kostić-Vuković J, Jovanović J, Simonović P, Simić V, Piria M, Gačić Z, Lenhardt M, Paunović M, Vuković-Gačić B. The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study. in International Journal of Environmental Research. 2018;.
doi:10.1007/s41742-018-0127-6 .

Kolarević, Stoimir, Sunjog, Karolina, Kračun-Kolarević, Margareta, Kostić-Vuković, Jovana, Jovanović, Jovana, Simonović, Predrag, Simić, Vladica, Piria, Marina, Gačić, Zoran, Lenhardt, Mirjana, Paunović, Momir, Vuković-Gačić, Branka, "The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study" in International Journal of Environmental Research (2018),
https://doi.org/10.1007/s41742-018-0127-6 . .

TY  - JOUR
AU  - Tokodi, Nada
AU  - Drobac, Damjana
AU  - Meriluoto, Jussi
AU  - Lujić, Jelena
AU  - Marinović, Zoran
AU  - Važić, Tamara
AU  - Nybom, Sonja
AU  - Simeunović, Jelica
AU  - Dulić, Tamara
AU  - Lazić, Gospava
AU  - Petrović, Tamaš
AU  - Vuković-Gačić, Branka
AU  - Sunjog, Karolina
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Subakov-Simić, Gordana
AU  - Miljanović, Branko
AU  - Codd, Geoffrey A.
AU  - Svirčev, Zorica
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0048969718313512
UR  - http://www.ncbi.nlm.nih.gov/pubmed/29710560
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3046
AB  - Cyanobacteria are present in many aquatic ecosystems in Serbia. Lake Ludoš, a wetland area of international significance and an important habitat for waterbirds, has become the subject of intense research interest because of practically continuous blooming of cyanobacteria. Analyses of water samples indicated a deterioration of ecological condition and water quality, and the presence of toxin-producing cyanobacteria (the most abundant Limnothrix redekei, Pseudanabaena limnetica, Planktothrix agardhii and Microcystis spp.). Furthermore, microcystins were detected in plants and animals from the lake: in macrophyte rhizomes (Phragmites communis, Typha latifolia and Nymphaea elegans), and in the muscle, intestines, kidneys, gonads and gills of fish (Carassius gibelio). Moreover, histopathological deleterious effects (liver, kidney, gills and intestines) and DNA damage (liver and gills) were observed in fish. A potential treatment for the reduction of cyanobacterial populations employing hydrogen peroxide was tested during this study. The treatment was not effective in laboratory tests although further in-lake trials are needed to make final conclusions about the applicability of the method. Based on our observations of the cyanobacterial populations and cyanotoxins in the water, as well as other aquatic organisms and, a survey of historical data on Lake Ludoš, it can be concluded that the lake is continuously in a poor ecological state. Conservation of the lake in order to protect the waterbirds (without urgent control of eutrophication) actually endangers them and the rest of the biota in this wetland habitat, and possibly other ecosystems. Thus, urgent measures for restoration are required, so that the preservation of this Ramsar site would be meaningful.
PB  - Elsevier B.V.
T2  - Science of the Total Environment
T1  - Cyanobacterial effects in Lake Ludoš, Serbia - Is preservation of a degraded aquatic ecosystem justified?
VL  - 635
DO  - 10.1016/j.scitotenv.2018.04.177
SP  - 1047
EP  - 1062
ER  - 

@article{
author = "Tokodi, Nada and Drobac, Damjana and Meriluoto, Jussi and Lujić, Jelena and Marinović, Zoran and Važić, Tamara and Nybom, Sonja and Simeunović, Jelica and Dulić, Tamara and Lazić, Gospava and Petrović, Tamaš and Vuković-Gačić, Branka and Sunjog, Karolina and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Subakov-Simić, Gordana and Miljanović, Branko and Codd, Geoffrey A. and Svirčev, Zorica",
year = "2018",
abstract = "Cyanobacteria are present in many aquatic ecosystems in Serbia. Lake Ludoš, a wetland area of international significance and an important habitat for waterbirds, has become the subject of intense research interest because of practically continuous blooming of cyanobacteria. Analyses of water samples indicated a deterioration of ecological condition and water quality, and the presence of toxin-producing cyanobacteria (the most abundant Limnothrix redekei, Pseudanabaena limnetica, Planktothrix agardhii and Microcystis spp.). Furthermore, microcystins were detected in plants and animals from the lake: in macrophyte rhizomes (Phragmites communis, Typha latifolia and Nymphaea elegans), and in the muscle, intestines, kidneys, gonads and gills of fish (Carassius gibelio). Moreover, histopathological deleterious effects (liver, kidney, gills and intestines) and DNA damage (liver and gills) were observed in fish. A potential treatment for the reduction of cyanobacterial populations employing hydrogen peroxide was tested during this study. The treatment was not effective in laboratory tests although further in-lake trials are needed to make final conclusions about the applicability of the method. Based on our observations of the cyanobacterial populations and cyanotoxins in the water, as well as other aquatic organisms and, a survey of historical data on Lake Ludoš, it can be concluded that the lake is continuously in a poor ecological state. Conservation of the lake in order to protect the waterbirds (without urgent control of eutrophication) actually endangers them and the rest of the biota in this wetland habitat, and possibly other ecosystems. Thus, urgent measures for restoration are required, so that the preservation of this Ramsar site would be meaningful.",
publisher = "Elsevier B.V.",
journal = "Science of the Total Environment",
title = "Cyanobacterial effects in Lake Ludoš, Serbia - Is preservation of a degraded aquatic ecosystem justified?",
volume = "635",
doi = "10.1016/j.scitotenv.2018.04.177",
pages = "1047-1062"
}

Tokodi, N., Drobac, D., Meriluoto, J., Lujić, J., Marinović, Z., Važić, T., Nybom, S., Simeunović, J., Dulić, T., Lazić, G., Petrović, T., Vuković-Gačić, B., Sunjog, K., Kolarević, S., Kračun-Kolarević, M., Subakov-Simić, G., Miljanović, B., Codd, G. A.,& Svirčev, Z.. (2018). Cyanobacterial effects in Lake Ludoš, Serbia - Is preservation of a degraded aquatic ecosystem justified?. in Science of the Total Environment
Elsevier B.V.., 635, 1047-1062.
https://doi.org/10.1016/j.scitotenv.2018.04.177

Tokodi N, Drobac D, Meriluoto J, Lujić J, Marinović Z, Važić T, Nybom S, Simeunović J, Dulić T, Lazić G, Petrović T, Vuković-Gačić B, Sunjog K, Kolarević S, Kračun-Kolarević M, Subakov-Simić G, Miljanović B, Codd GA, Svirčev Z. Cyanobacterial effects in Lake Ludoš, Serbia - Is preservation of a degraded aquatic ecosystem justified?. in Science of the Total Environment. 2018;635:1047-1062.
doi:10.1016/j.scitotenv.2018.04.177 .

Tokodi, Nada, Drobac, Damjana, Meriluoto, Jussi, Lujić, Jelena, Marinović, Zoran, Važić, Tamara, Nybom, Sonja, Simeunović, Jelica, Dulić, Tamara, Lazić, Gospava, Petrović, Tamaš, Vuković-Gačić, Branka, Sunjog, Karolina, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Subakov-Simić, Gordana, Miljanović, Branko, Codd, Geoffrey A., Svirčev, Zorica, "Cyanobacterial effects in Lake Ludoš, Serbia - Is preservation of a degraded aquatic ecosystem justified?" in Science of the Total Environment, 635 (2018):1047-1062,
https://doi.org/10.1016/j.scitotenv.2018.04.177 . .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Milovanović, Dragana
AU  - Kračun-Kolarević, Margareta
AU  - Kostić, Jovana
AU  - Sunjog, Karolina
AU  - Martinović, Rajko
AU  - Đorđević, Jelena
AU  - Novaković, Irena
AU  - Sladić, Dušan
AU  - Vuković-Gačić, Branka
PY  - 2018
UR  - https://www.tandfonline.com/doi/full/10.1080/01480545.2017.1413108
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2966
AB  - In this study, mutagenic and genotoxic potential of anti-tumor compounds avarol, avarone, and its derivatives 3'-methoxyavarone, 4'-(methylamino)avarone and 3'-(methylamino)avarone was evaluated and compared to cytostatics commonly used in chemotherapy (5-fluorouracil, etoposid, and cisplatin). Mutagenic potential of selected hydroquinone and quinones was assessed in prokaryotic model by the SOS/umuC assay in Salmonella typhimurium TA1535/pSK1002. Genotoxic potential was also assessed in eukaryotic models using comet assay in human fetal lung cell line (MRC-5), human adenocarcinoma epithelial cell line (A549), and in human peripheral blood cells (HPBC). The results indicated that avarol and avarone do not exert mutagenic/genotoxic potential. Among the studied avarone derivatives, mutagenic potential was detected by SOS/umuC test for 3'-(methylamino)avarone, but only after metabolic activation. The results of comet assay indicated that 3'-methoxyavarone and 3'-(methylamino)avarone have a significant impact on the level of DNA damage in the MRC-5 cell line. Genotoxic potential was not observed in A549 cells or HPBC probably due to a different uptake rate for the compounds and lower in metabolism rate within these cells.
T2  - Drug and Chemical Toxicology
T1  - Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models.
IS  - 2
VL  - 42
DO  - 10.1080/01480545.2017.1413108
SP  - 130
EP  - 139
ER  - 

@article{
author = "Kolarević, Stoimir and Milovanović, Dragana and Kračun-Kolarević, Margareta and Kostić, Jovana and Sunjog, Karolina and Martinović, Rajko and Đorđević, Jelena and Novaković, Irena and Sladić, Dušan and Vuković-Gačić, Branka",
year = "2018",
abstract = "In this study, mutagenic and genotoxic potential of anti-tumor compounds avarol, avarone, and its derivatives 3'-methoxyavarone, 4'-(methylamino)avarone and 3'-(methylamino)avarone was evaluated and compared to cytostatics commonly used in chemotherapy (5-fluorouracil, etoposid, and cisplatin). Mutagenic potential of selected hydroquinone and quinones was assessed in prokaryotic model by the SOS/umuC assay in Salmonella typhimurium TA1535/pSK1002. Genotoxic potential was also assessed in eukaryotic models using comet assay in human fetal lung cell line (MRC-5), human adenocarcinoma epithelial cell line (A549), and in human peripheral blood cells (HPBC). The results indicated that avarol and avarone do not exert mutagenic/genotoxic potential. Among the studied avarone derivatives, mutagenic potential was detected by SOS/umuC test for 3'-(methylamino)avarone, but only after metabolic activation. The results of comet assay indicated that 3'-methoxyavarone and 3'-(methylamino)avarone have a significant impact on the level of DNA damage in the MRC-5 cell line. Genotoxic potential was not observed in A549 cells or HPBC probably due to a different uptake rate for the compounds and lower in metabolism rate within these cells.",
journal = "Drug and Chemical Toxicology",
title = "Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models.",
number = "2",
volume = "42",
doi = "10.1080/01480545.2017.1413108",
pages = "130-139"
}

Kolarević, S., Milovanović, D., Kračun-Kolarević, M., Kostić, J., Sunjog, K., Martinović, R., Đorđević, J., Novaković, I., Sladić, D.,& Vuković-Gačić, B.. (2018). Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models.. in Drug and Chemical Toxicology, 42(2), 130-139.
https://doi.org/10.1080/01480545.2017.1413108

Kolarević S, Milovanović D, Kračun-Kolarević M, Kostić J, Sunjog K, Martinović R, Đorđević J, Novaković I, Sladić D, Vuković-Gačić B. Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models.. in Drug and Chemical Toxicology. 2018;42(2):130-139.
doi:10.1080/01480545.2017.1413108 .

Kolarević, Stoimir, Milovanović, Dragana, Kračun-Kolarević, Margareta, Kostić, Jovana, Sunjog, Karolina, Martinović, Rajko, Đorđević, Jelena, Novaković, Irena, Sladić, Dušan, Vuković-Gačić, Branka, "Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models." in Drug and Chemical Toxicology, 42, no. 2 (2018):130-139,
https://doi.org/10.1080/01480545.2017.1413108 . .

TY  - CHAP
AU  - Vuković-Gačić, Branka
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Tomović, Jelena
AU  - Kračun-Kolarević, Margareta
AU  - Knežević-Vukčević, Jelena
AU  - Paunović, Momir
AU  - Gačić, Zoran
PY  - 2015
UR  - http://link.springer.com/10.1007/978-3-662-44034-6
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2579
AB  - Genotoxicity monitoring of the lower stretch of the Sava River was performed by the combined approach of in situ assessment of genotoxicity and active biomonitoring of two species of mussels from the Unionidae family, Unio pictorum and Unio tumidus. Genotoxic response was studied using comet assay on hemocytes. For active biomonitoring, the mussels were acclimated to controlled laboratory conditions for 10 days and then exposed at two sites in the Sava River in the area of the city of Belgrade. Hemolymph of exposed specimens of each species was taken after 7, 14, and 30 days of exposure. For in situ assessment, the mussels were collected from five sites in the lower flow of the Sava River. The mussels were sampled immediately after the acclimation served as controls in both types of monitoring procedures. The results of our studies indicated the presence of genotoxic pollution at all studied sites at the Sava River. The level of DNA damage varied at different sites depending on the source and level of pollution. The response to genotoxic pollution was evident at the site in the urban area of Belgrade city, as well as at the sites far from the large urban settlements, suggesting that the lower flow of the Sava River is under pollution pressure.
PB  - Springer-Verlag, Berlin, Heidelberg
T2  - The Sava River
T1  - Genotoxicological Studies of Lower Stretch of the Sava River
VL  - 31
DO  - 10.1007/978-3-662-44034-6
SP  - 437
EP  - 452
ER  - 

@inbook{
editor = "Milačič, Radmila, Ščančar, Janez, Paunović, Momir",
author = "Vuković-Gačić, Branka and Kolarević, Stoimir and Sunjog, Karolina and Tomović, Jelena and Kračun-Kolarević, Margareta and Knežević-Vukčević, Jelena and Paunović, Momir and Gačić, Zoran",
year = "2015",
abstract = "Genotoxicity monitoring of the lower stretch of the Sava River was performed by the combined approach of in situ assessment of genotoxicity and active biomonitoring of two species of mussels from the Unionidae family, Unio pictorum and Unio tumidus. Genotoxic response was studied using comet assay on hemocytes. For active biomonitoring, the mussels were acclimated to controlled laboratory conditions for 10 days and then exposed at two sites in the Sava River in the area of the city of Belgrade. Hemolymph of exposed specimens of each species was taken after 7, 14, and 30 days of exposure. For in situ assessment, the mussels were collected from five sites in the lower flow of the Sava River. The mussels were sampled immediately after the acclimation served as controls in both types of monitoring procedures. The results of our studies indicated the presence of genotoxic pollution at all studied sites at the Sava River. The level of DNA damage varied at different sites depending on the source and level of pollution. The response to genotoxic pollution was evident at the site in the urban area of Belgrade city, as well as at the sites far from the large urban settlements, suggesting that the lower flow of the Sava River is under pollution pressure.",
publisher = "Springer-Verlag, Berlin, Heidelberg",
journal = "The Sava River",
booktitle = "Genotoxicological Studies of Lower Stretch of the Sava River",
volume = "31",
doi = "10.1007/978-3-662-44034-6",
pages = "437-452"
}

Milačič, R., Ščančar, J., Paunović, M., Vuković-Gačić, B., Kolarević, S., Sunjog, K., Tomović, J., Kračun-Kolarević, M., Knežević-Vukčević, J., Paunović, M.,& Gačić, Z.. (2015). Genotoxicological Studies of Lower Stretch of the Sava River. in The Sava River
Springer-Verlag, Berlin, Heidelberg., 31, 437-452.
https://doi.org/10.1007/978-3-662-44034-6

Milačič R, Ščančar J, Paunović M, Vuković-Gačić B, Kolarević S, Sunjog K, Tomović J, Kračun-Kolarević M, Knežević-Vukčević J, Paunović M, Gačić Z. Genotoxicological Studies of Lower Stretch of the Sava River. in The Sava River. 2015;31:437-452.
doi:10.1007/978-3-662-44034-6 .

Milačič, Radmila, Ščančar, Janez, Paunović, Momir, Vuković-Gačić, Branka, Kolarević, Stoimir, Sunjog, Karolina, Tomović, Jelena, Kračun-Kolarević, Margareta, Knežević-Vukčević, Jelena, Paunović, Momir, Gačić, Zoran, "Genotoxicological Studies of Lower Stretch of the Sava River" in The Sava River, 31 (2015):437-452,
https://doi.org/10.1007/978-3-662-44034-6 . .

TY  - JOUR
AU  - Vukovic-Gacic, Branka
AU  - Kolarevic, Stoimir
AU  - Sunjog, Karolina
AU  - Tomović, Jelena
AU  - Knežević-Vukčević, Jelena
AU  - Paunović, Momir
AU  - Gacic, Zoran
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2174
AB  - Genotoxic response of freshwater mussels U. tumidus and U. pictorum to
   environmental stress was studied using comet assay on hemocytes and gill
   cells. The mussels were acclimated to controlled laboratory conditions
   for 10 days, and then exposed at 4 sites in the Sava and Danube rivers
   in the area of the city of Belgrade. Samples of each species were taken
   after 7, 14, and 30 days of exposure. The mussels sampled immediately
   after acclimation served as controls. Genotoxic response in both species
   was induced earlier at sites receiving untreated wastewaters from the
   city's main collectors (7 days), than at the site receiving only
   domestic wastewaters from small settlements located upstream from the
   city (30 days). There was a correlation between the comet tail intensity
   values in tissues of exposed mussels and the concentrations of zinc,
   copper, iron, and arsenic at the exposure sites. The genotoxic responses
   in both tissues of U. pictorum and in hemocytes of U. tumidus were
   similar, while U. tumidus gill cells failed to exhibit significant
   genotoxic response at two sites. These findings, together with higher
   mortality of U. tumidus at the most polluted sites, promote U. pictorum
   as a model for genotoxicity monitoring in freshwater environments.
T2  - Hydrobiologia
T1  - Comparative study of the genotoxic response of freshwater mussels Unio
 tumidus and Unio pictorum to environmental stress
IS  - 1
VL  - 735
DO  - 10.1007/s10750-013-1513-x
SP  - 221
EP  - 231
ER  - 

@article{
author = "Vukovic-Gacic, Branka and Kolarevic, Stoimir and Sunjog, Karolina and Tomović, Jelena and Knežević-Vukčević, Jelena and Paunović, Momir and Gacic, Zoran",
year = "2014",
abstract = "Genotoxic response of freshwater mussels U. tumidus and U. pictorum to
   environmental stress was studied using comet assay on hemocytes and gill
   cells. The mussels were acclimated to controlled laboratory conditions
   for 10 days, and then exposed at 4 sites in the Sava and Danube rivers
   in the area of the city of Belgrade. Samples of each species were taken
   after 7, 14, and 30 days of exposure. The mussels sampled immediately
   after acclimation served as controls. Genotoxic response in both species
   was induced earlier at sites receiving untreated wastewaters from the
   city's main collectors (7 days), than at the site receiving only
   domestic wastewaters from small settlements located upstream from the
   city (30 days). There was a correlation between the comet tail intensity
   values in tissues of exposed mussels and the concentrations of zinc,
   copper, iron, and arsenic at the exposure sites. The genotoxic responses
   in both tissues of U. pictorum and in hemocytes of U. tumidus were
   similar, while U. tumidus gill cells failed to exhibit significant
   genotoxic response at two sites. These findings, together with higher
   mortality of U. tumidus at the most polluted sites, promote U. pictorum
   as a model for genotoxicity monitoring in freshwater environments.",
journal = "Hydrobiologia",
title = "Comparative study of the genotoxic response of freshwater mussels Unio
 tumidus and Unio pictorum to environmental stress",
number = "1",
volume = "735",
doi = "10.1007/s10750-013-1513-x",
pages = "221-231"
}

Vukovic-Gacic, B., Kolarevic, S., Sunjog, K., Tomović, J., Knežević-Vukčević, J., Paunović, M.,& Gacic, Z.. (2014). Comparative study of the genotoxic response of freshwater mussels Unio
 tumidus and Unio pictorum to environmental stress. in Hydrobiologia, 735(1), 221-231.
https://doi.org/10.1007/s10750-013-1513-x

Vukovic-Gacic B, Kolarevic S, Sunjog K, Tomović J, Knežević-Vukčević J, Paunović M, Gacic Z. Comparative study of the genotoxic response of freshwater mussels Unio
 tumidus and Unio pictorum to environmental stress. in Hydrobiologia. 2014;735(1):221-231.
doi:10.1007/s10750-013-1513-x .

Vukovic-Gacic, Branka, Kolarevic, Stoimir, Sunjog, Karolina, Tomović, Jelena, Knežević-Vukčević, Jelena, Paunović, Momir, Gacic, Zoran, "Comparative study of the genotoxic response of freshwater mussels Unio
 tumidus and Unio pictorum to environmental stress" in Hydrobiologia, 735, no. 1 (2014):221-231,
https://doi.org/10.1007/s10750-013-1513-x . .

TY  - JOUR
AU  - Heberger, Karoly
AU  - Kolarevic, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Sunjog, Karolina
AU  - Gacic, Zoran
AU  - Kljajic, Zoran
AU  - Mitric, Milena
AU  - Vukovic-Gacic, Branka
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2165
AB  - Specimens of the mussel Mytilus galloprovincialis were collected from
   five sites in the Boka Kotorska Bay (Adriatic Sea, Montenegro) during
   the period summer 2011-autumn 2012. Three types of tissue, haemolymph,
   digestive gland were used for assessment of DNA damage. Images of
   randomly selected cells were analyzed with a fluorescence microscope and
   image analysis by the Comet Assay IV Image-analysis system. Three
   parameters, viz, tail length, tail intensity and Olive tail moment were
   analyzed on 4200 nuclei per cell type. We observed variations in the
   level of DNA damage in mussels collected at different sites, as well as
   seasonal variations in response. Sum of ranking differences (SRD) was
   implemented to compare use of different types of cell and different
   measure of comet tail per nucleus. Numerical scales were transferred
   into ranks, range scaling between 0 and 1; standardization and
   normalization were carried out.
   SRD selected the best (and worst) combinations: tail moment is the best
   for all data treatment and for all organs; second best is tail length,
   and intensity ranks third (except for digestive gland). The differences
   were significant at the 5\% level. Whereas gills and haemolymph cells do
   not differ significantly, cells of the digestive gland are much more
   suitable to estimate genotoxicity. Variance analysis decomposed the
   effect of different factors on the SRD values. This unique combination
   has provided not only the relative importance of factors, but also an
   overall evaluation: the best evaluation method, the best data
   pre-treatment, etc., were chosen even for partially contradictory data.
   The rank transformation is superior to any other way of scaling, which
   is proven by ordering the SRD values by SRD again, and by cross
   validation. (C) 2014 Elsevier B.V. All rights reserved.
T2  - Mutation Research-Genetic Toxicology and Environmental Mutagenesis
T1  - Evaluation of single-cell gel electrophoresis data: Combination of
 variance analysis with sum of ranking differences
VL  - 771
DO  - 10.1016/j.mrgentox.2014.04.028
SP  - 15
EP  - 22
ER  - 

@article{
author = "Heberger, Karoly and Kolarevic, Stoimir and Kračun-Kolarević, Margareta and Sunjog, Karolina and Gacic, Zoran and Kljajic, Zoran and Mitric, Milena and Vukovic-Gacic, Branka",
year = "2014",
abstract = "Specimens of the mussel Mytilus galloprovincialis were collected from
   five sites in the Boka Kotorska Bay (Adriatic Sea, Montenegro) during
   the period summer 2011-autumn 2012. Three types of tissue, haemolymph,
   digestive gland were used for assessment of DNA damage. Images of
   randomly selected cells were analyzed with a fluorescence microscope and
   image analysis by the Comet Assay IV Image-analysis system. Three
   parameters, viz, tail length, tail intensity and Olive tail moment were
   analyzed on 4200 nuclei per cell type. We observed variations in the
   level of DNA damage in mussels collected at different sites, as well as
   seasonal variations in response. Sum of ranking differences (SRD) was
   implemented to compare use of different types of cell and different
   measure of comet tail per nucleus. Numerical scales were transferred
   into ranks, range scaling between 0 and 1; standardization and
   normalization were carried out.
   SRD selected the best (and worst) combinations: tail moment is the best
   for all data treatment and for all organs; second best is tail length,
   and intensity ranks third (except for digestive gland). The differences
   were significant at the 5\% level. Whereas gills and haemolymph cells do
   not differ significantly, cells of the digestive gland are much more
   suitable to estimate genotoxicity. Variance analysis decomposed the
   effect of different factors on the SRD values. This unique combination
   has provided not only the relative importance of factors, but also an
   overall evaluation: the best evaluation method, the best data
   pre-treatment, etc., were chosen even for partially contradictory data.
   The rank transformation is superior to any other way of scaling, which
   is proven by ordering the SRD values by SRD again, and by cross
   validation. (C) 2014 Elsevier B.V. All rights reserved.",
journal = "Mutation Research-Genetic Toxicology and Environmental Mutagenesis",
title = "Evaluation of single-cell gel electrophoresis data: Combination of
 variance analysis with sum of ranking differences",
volume = "771",
doi = "10.1016/j.mrgentox.2014.04.028",
pages = "15-22"
}

Heberger, K., Kolarevic, S., Kračun-Kolarević, M., Sunjog, K., Gacic, Z., Kljajic, Z., Mitric, M.,& Vukovic-Gacic, B.. (2014). Evaluation of single-cell gel electrophoresis data: Combination of
 variance analysis with sum of ranking differences. in Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 771, 15-22.
https://doi.org/10.1016/j.mrgentox.2014.04.028

Heberger K, Kolarevic S, Kračun-Kolarević M, Sunjog K, Gacic Z, Kljajic Z, Mitric M, Vukovic-Gacic B. Evaluation of single-cell gel electrophoresis data: Combination of
 variance analysis with sum of ranking differences. in Mutation Research-Genetic Toxicology and Environmental Mutagenesis. 2014;771:15-22.
doi:10.1016/j.mrgentox.2014.04.028 .

Heberger, Karoly, Kolarevic, Stoimir, Kračun-Kolarević, Margareta, Sunjog, Karolina, Gacic, Zoran, Kljajic, Zoran, Mitric, Milena, Vukovic-Gacic, Branka, "Evaluation of single-cell gel electrophoresis data: Combination of
 variance analysis with sum of ranking differences" in Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 771 (2014):15-22,
https://doi.org/10.1016/j.mrgentox.2014.04.028 . .