TY  - JOUR
AU  - Miljković, Đorđe
AU  - Spasojević, Ivan B
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/937
AB  - The pathophysiology of multiple sclerosis (MS) involves several components: redox, inflammatory/autoimmune, vascular, and neurodegenerative. All of them are supported by the intertwined lines of evidence, and none of them should be written off. However, the exact mechanisms of MS initiation, its development, and progression are still elusive, despite the impressive pace by which the data on MS are accumulating. In this review, we will try to integrate the current facts and concepts, focusing on the role of redox changes and various reactive species in MS. Knowing the schedule of initial changes in pathogenic factors and the key turning points, as well as understanding the redox processes involved in MS pathogenesis is the way to enable MS prevention, early treatment, and the development of therapies that target specific pathophysiological components of the heterogeneous mechanisms of MS, which could alleviate the symptoms and hopefully stop MS. Pertinent to this, we will outline (i) redox processes involved in MS initiation; (ii) the role of reactive species in inflammation; (iii) prooxidative changes responsible for neurodegeneration; and (iv) the potential of antioxidative therapy. Antioxid. Redox Signal. 19, 2286-2334.
PB  - New Rochelle: Mary Ann Liebert, Inc.
T2  - Antioxidants & Redox Signaling
T1  - Multiple Sclerosis: Molecular Mechanisms and Therapeutic Opportunities
IS  - 18
VL  - 19
DO  - 10.1089/ars.2012.5068
SP  - 2286
EP  - 2334
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_937
ER  - 

@article{
author = "Miljković, Đorđe and Spasojević, Ivan B",
year = "2013",
abstract = "The pathophysiology of multiple sclerosis (MS) involves several components: redox, inflammatory/autoimmune, vascular, and neurodegenerative. All of them are supported by the intertwined lines of evidence, and none of them should be written off. However, the exact mechanisms of MS initiation, its development, and progression are still elusive, despite the impressive pace by which the data on MS are accumulating. In this review, we will try to integrate the current facts and concepts, focusing on the role of redox changes and various reactive species in MS. Knowing the schedule of initial changes in pathogenic factors and the key turning points, as well as understanding the redox processes involved in MS pathogenesis is the way to enable MS prevention, early treatment, and the development of therapies that target specific pathophysiological components of the heterogeneous mechanisms of MS, which could alleviate the symptoms and hopefully stop MS. Pertinent to this, we will outline (i) redox processes involved in MS initiation; (ii) the role of reactive species in inflammation; (iii) prooxidative changes responsible for neurodegeneration; and (iv) the potential of antioxidative therapy. Antioxid. Redox Signal. 19, 2286-2334.",
publisher = "New Rochelle: Mary Ann Liebert, Inc.",
journal = "Antioxidants & Redox Signaling",
title = "Multiple Sclerosis: Molecular Mechanisms and Therapeutic Opportunities",
number = "18",
volume = "19",
doi = "10.1089/ars.2012.5068",
pages = "2286-2334",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_937"
}

Miljković, Đ.,& Spasojević, I. B.. (2013). Multiple Sclerosis: Molecular Mechanisms and Therapeutic Opportunities. in Antioxidants & Redox Signaling
New Rochelle: Mary Ann Liebert, Inc.., 19(18), 2286-2334.
https://doi.org/10.1089/ars.2012.5068
https://hdl.handle.net/21.15107/rcub_ibiss_937

Miljković Đ, Spasojević IB. Multiple Sclerosis: Molecular Mechanisms and Therapeutic Opportunities. in Antioxidants & Redox Signaling. 2013;19(18):2286-2334.
doi:10.1089/ars.2012.5068
https://hdl.handle.net/21.15107/rcub_ibiss_937 .

Miljković, Đorđe, Spasojević, Ivan B, "Multiple Sclerosis: Molecular Mechanisms and Therapeutic Opportunities" in Antioxidants & Redox Signaling, 19, no. 18 (2013):2286-2334,
https://doi.org/10.1089/ars.2012.5068 .,
https://hdl.handle.net/21.15107/rcub_ibiss_937 .

TY  - JOUR
AU  - Bajić, Aleksandar
AU  - Spasić, Mihajlo
AU  - Anđus, Pavle R
AU  - Savić, Danijela
AU  - Parabučki, Ana B.
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasojević, Ivan B
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/959
AB  - The effects of H2O2 are widely studied in cell cultures and other in vitro systems. However, such investigations are performed with the assumption that H2O2 concentration is constant, which may not properly reflect in vivo settings, particularly in redox-turbulent microenvironments such as mitochondria. Here we introduced and tested a novel concept of fluctuating oxidative stress. We treated C6 astroglial cells and primary astrocytes with H2O2, using three regimes of exposure - continuous, as well as fluctuating at low or high rate, and evaluated mitochondrial membrane potential and other parameters of mitochondrial activity - respiration, reducing capacity, and superoxide production, as well as intracellular ATP, intracellular calcium, and NF-kappa B activation. When compared to continuous exposure, fluctuating H2O2 induced a pronounced hyperpolarization in mitochondria, whereas the activity of electron transport chain appears not to be significantly affected. H2O2 provoked a decrease of ATP level and an increase of intracellular calcium concentration, independently of the regime of treatment. However, fluctuating H2O2 induced a specific pattern of large-amplitude fluctuations of calcium concentration. An impact on NF kappa B activation was observed for high rate fluctuations, whereas continuous and low rate fluctuating oxidative stress did not provoke significant effects. Presented results outline the (patho)physiological relevance of redox fluctuations.
T2  - Plos One
T1  - Fluctuating vs. Continuous Exposure to H2O2: The Effects on Mitochondrial Membrane Potential, Intracellular Calcium, and NF-kappa B in Astroglia
IS  - 10
VL  - 8
SP  - 111
EP  - na
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_959
ER  - 

@article{
author = "Bajić, Aleksandar and Spasić, Mihajlo and Anđus, Pavle R and Savić, Danijela and Parabučki, Ana B. and Nikolić-Kokić, Aleksandra and Spasojević, Ivan B",
year = "2013",
abstract = "The effects of H2O2 are widely studied in cell cultures and other in vitro systems. However, such investigations are performed with the assumption that H2O2 concentration is constant, which may not properly reflect in vivo settings, particularly in redox-turbulent microenvironments such as mitochondria. Here we introduced and tested a novel concept of fluctuating oxidative stress. We treated C6 astroglial cells and primary astrocytes with H2O2, using three regimes of exposure - continuous, as well as fluctuating at low or high rate, and evaluated mitochondrial membrane potential and other parameters of mitochondrial activity - respiration, reducing capacity, and superoxide production, as well as intracellular ATP, intracellular calcium, and NF-kappa B activation. When compared to continuous exposure, fluctuating H2O2 induced a pronounced hyperpolarization in mitochondria, whereas the activity of electron transport chain appears not to be significantly affected. H2O2 provoked a decrease of ATP level and an increase of intracellular calcium concentration, independently of the regime of treatment. However, fluctuating H2O2 induced a specific pattern of large-amplitude fluctuations of calcium concentration. An impact on NF kappa B activation was observed for high rate fluctuations, whereas continuous and low rate fluctuating oxidative stress did not provoke significant effects. Presented results outline the (patho)physiological relevance of redox fluctuations.",
journal = "Plos One",
title = "Fluctuating vs. Continuous Exposure to H2O2: The Effects on Mitochondrial Membrane Potential, Intracellular Calcium, and NF-kappa B in Astroglia",
number = "10",
volume = "8",
pages = "111-na",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_959"
}

Bajić, A., Spasić, M., Anđus, P. R., Savić, D., Parabučki, A. B., Nikolić-Kokić, A.,& Spasojević, I. B.. (2013). Fluctuating vs. Continuous Exposure to H2O2: The Effects on Mitochondrial Membrane Potential, Intracellular Calcium, and NF-kappa B in Astroglia. in Plos One, 8(10), 111-na.
https://hdl.handle.net/21.15107/rcub_ibiss_959

Bajić A, Spasić M, Anđus PR, Savić D, Parabučki AB, Nikolić-Kokić A, Spasojević IB. Fluctuating vs. Continuous Exposure to H2O2: The Effects on Mitochondrial Membrane Potential, Intracellular Calcium, and NF-kappa B in Astroglia. in Plos One. 2013;8(10):111-na.
https://hdl.handle.net/21.15107/rcub_ibiss_959 .

Bajić, Aleksandar, Spasić, Mihajlo, Anđus, Pavle R, Savić, Danijela, Parabučki, Ana B., Nikolić-Kokić, Aleksandra, Spasojević, Ivan B, "Fluctuating vs. Continuous Exposure to H2O2: The Effects on Mitochondrial Membrane Potential, Intracellular Calcium, and NF-kappa B in Astroglia" in Plos One, 8, no. 10 (2013):111-na,
https://hdl.handle.net/21.15107/rcub_ibiss_959 .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Mojić, Marija
AU  - Maksimović-Ivanić, Danijela
AU  - Bulatović, Mirna Z.
AU  - Mijatović, Sanja
AU  - Milošević, Verica
AU  - Spasojević, Ivan B
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1024
AB  - Soy isoflavones represent hopeful unconventional remedies in the therapy of prostate cancer. The aim of our study was to determine the effects of genistein and daidzein on the parameters that reflect metastatic potential, membrane fluidity, invasiveness and dynamic phenotype in Matrigel of LNCaP and PC-3 prostate cancer cells. Cell viability tests, using a wide range of concentrations of soy isoflavones (6-75 mu g/ml for 72 h), were conducted to determine their IC50 concentrations. Electron paramagnetic resonance investigations of prostate cancer cell membrane fluidity were performed at IC50 concentrations of genistein and daidzein (12.5 and 25 mu g/ml, respectively, for 10 min). Genistein provoked significant increases in the membrane order parameter (which is reciprocally proportional to membrane fluidity) of 0.722 +/- A 0.006 (LNCaP), 0.753 +/- A 0.010 (LNCaP + genistein), 0.723 +/- A 0.007 (PC-3) and 0.741 +/- A 0.004 (PC-3 + genistein); however, no such effects were observed for daidzein. While both genistein and daidzein reduced the proliferation of prostate cancer cells at their respective IC50 concentrations, during the 72 h of incubation only genistein provoked effects on the dynamic phenotype and decreased invasiveness. The effect was more evident in PC-3 cells compared to LNCaP cells. Our results imply that (1) invasive activity is at least partially dependent on membrane fluidity, (2) genistein may exert its antimetastatic effects by changing the mechanical properties of prostate cancer cells and (3) daidzein should be applied at higher concentrations than genistein in order to achieve pharmacological effects.
T2  - Journal of Membrane Biology
T1  - Membrane Fluidity, Invasiveness and Dynamic Phenotype of Metastatic Prostate Cancer Cells after Treatment with Soy Isoflavones
IS  - 4
VL  - 246
SP  - 145
EP  - 314
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1024
ER  - 

@article{
author = "Ajdžanović, Vladimir and Mojić, Marija and Maksimović-Ivanić, Danijela and Bulatović, Mirna Z. and Mijatović, Sanja and Milošević, Verica and Spasojević, Ivan B",
year = "2013",
abstract = "Soy isoflavones represent hopeful unconventional remedies in the therapy of prostate cancer. The aim of our study was to determine the effects of genistein and daidzein on the parameters that reflect metastatic potential, membrane fluidity, invasiveness and dynamic phenotype in Matrigel of LNCaP and PC-3 prostate cancer cells. Cell viability tests, using a wide range of concentrations of soy isoflavones (6-75 mu g/ml for 72 h), were conducted to determine their IC50 concentrations. Electron paramagnetic resonance investigations of prostate cancer cell membrane fluidity were performed at IC50 concentrations of genistein and daidzein (12.5 and 25 mu g/ml, respectively, for 10 min). Genistein provoked significant increases in the membrane order parameter (which is reciprocally proportional to membrane fluidity) of 0.722 +/- A 0.006 (LNCaP), 0.753 +/- A 0.010 (LNCaP + genistein), 0.723 +/- A 0.007 (PC-3) and 0.741 +/- A 0.004 (PC-3 + genistein); however, no such effects were observed for daidzein. While both genistein and daidzein reduced the proliferation of prostate cancer cells at their respective IC50 concentrations, during the 72 h of incubation only genistein provoked effects on the dynamic phenotype and decreased invasiveness. The effect was more evident in PC-3 cells compared to LNCaP cells. Our results imply that (1) invasive activity is at least partially dependent on membrane fluidity, (2) genistein may exert its antimetastatic effects by changing the mechanical properties of prostate cancer cells and (3) daidzein should be applied at higher concentrations than genistein in order to achieve pharmacological effects.",
journal = "Journal of Membrane Biology",
title = "Membrane Fluidity, Invasiveness and Dynamic Phenotype of Metastatic Prostate Cancer Cells after Treatment with Soy Isoflavones",
number = "4",
volume = "246",
pages = "145-314",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1024"
}

Ajdžanović, V., Mojić, M., Maksimović-Ivanić, D., Bulatović, M. Z., Mijatović, S., Milošević, V.,& Spasojević, I. B.. (2013). Membrane Fluidity, Invasiveness and Dynamic Phenotype of Metastatic Prostate Cancer Cells after Treatment with Soy Isoflavones. in Journal of Membrane Biology, 246(4), 145-314.
https://hdl.handle.net/21.15107/rcub_ibiss_1024

Ajdžanović V, Mojić M, Maksimović-Ivanić D, Bulatović MZ, Mijatović S, Milošević V, Spasojević IB. Membrane Fluidity, Invasiveness and Dynamic Phenotype of Metastatic Prostate Cancer Cells after Treatment with Soy Isoflavones. in Journal of Membrane Biology. 2013;246(4):145-314.
https://hdl.handle.net/21.15107/rcub_ibiss_1024 .

Ajdžanović, Vladimir, Mojić, Marija, Maksimović-Ivanić, Danijela, Bulatović, Mirna Z., Mijatović, Sanja, Milošević, Verica, Spasojević, Ivan B, "Membrane Fluidity, Invasiveness and Dynamic Phenotype of Metastatic Prostate Cancer Cells after Treatment with Soy Isoflavones" in Journal of Membrane Biology, 246, no. 4 (2013):145-314,
https://hdl.handle.net/21.15107/rcub_ibiss_1024 .

TY  - JOUR
AU  - Sonmez, Melda
AU  - Ince, Huseyin Yavuz
AU  - Yalcin, Ozlem
AU  - Ajdžanović, Vladimir
AU  - Spasojević, Ivan B
AU  - Meiselman, Herbert J
AU  - Baskurt, Oguz K
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/964
AB  - The role of membrane fluidity in determining red blood cell (RBC) deformability has been suggested by a number of studies. The present investigation evaluated alterations of RBC membrane fluidity, deformability and stability in the presence of four linear alcohols (methanol, ethanol, propanol and butanol) using ektacytometry and electron paramagnetic resonance (EPR) spectroscopy. All alcohols had a biphasic effect on deformability such that it increased then decreased with increasing concentration; the critical concentration for reversal was an inverse function of molecular size. EPR results showed biphasic changes of near-surface fluidity (i.e., increase then decrease) and a decreased fluidity of the lipid core; rank order of effectiveness was butanol > propanol > ethanol > methanol, with a significant correlation between near-surface fluidity and deformability (r = 0.697; p<0.01). The presence of alcohol enhanced the impairment of RBC deformability caused by subjecting cells to 100 Pa shear stress for 300 s, with significant differences from control being observed at higher concentrations of all four alcohols. The level of hemolysis was dependent on molecular size and concentration, whereas echinocytic shape transformation (i.e., biconcave disc to crenated morphology) was observed only for ethanol and propanol. These results are in accordance with available data obtained on model membranes. They document the presence of mechanical links between RBC deformability and near-surface membrane fluidity, chain length-dependence of the ability of alcohols to alter RBC mechanical behavior, and the biphasic response of RBC deformability and near-surface membrane fluidity to increasing alcohol concentrations.
T2  - Plos One
T1  - The Effect of Alcohols on Red Blood Cell Mechanical Properties and Membrane Fluidity Depends on Their Molecular Size
IS  - 9
VL  - 8
SP  - 106
EP  - na
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_964
ER  - 

@article{
author = "Sonmez, Melda and Ince, Huseyin Yavuz and Yalcin, Ozlem and Ajdžanović, Vladimir and Spasojević, Ivan B and Meiselman, Herbert J and Baskurt, Oguz K",
year = "2013",
abstract = "The role of membrane fluidity in determining red blood cell (RBC) deformability has been suggested by a number of studies. The present investigation evaluated alterations of RBC membrane fluidity, deformability and stability in the presence of four linear alcohols (methanol, ethanol, propanol and butanol) using ektacytometry and electron paramagnetic resonance (EPR) spectroscopy. All alcohols had a biphasic effect on deformability such that it increased then decreased with increasing concentration; the critical concentration for reversal was an inverse function of molecular size. EPR results showed biphasic changes of near-surface fluidity (i.e., increase then decrease) and a decreased fluidity of the lipid core; rank order of effectiveness was butanol > propanol > ethanol > methanol, with a significant correlation between near-surface fluidity and deformability (r = 0.697; p<0.01). The presence of alcohol enhanced the impairment of RBC deformability caused by subjecting cells to 100 Pa shear stress for 300 s, with significant differences from control being observed at higher concentrations of all four alcohols. The level of hemolysis was dependent on molecular size and concentration, whereas echinocytic shape transformation (i.e., biconcave disc to crenated morphology) was observed only for ethanol and propanol. These results are in accordance with available data obtained on model membranes. They document the presence of mechanical links between RBC deformability and near-surface membrane fluidity, chain length-dependence of the ability of alcohols to alter RBC mechanical behavior, and the biphasic response of RBC deformability and near-surface membrane fluidity to increasing alcohol concentrations.",
journal = "Plos One",
title = "The Effect of Alcohols on Red Blood Cell Mechanical Properties and Membrane Fluidity Depends on Their Molecular Size",
number = "9",
volume = "8",
pages = "106-na",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_964"
}

Sonmez, M., Ince, H. Y., Yalcin, O., Ajdžanović, V., Spasojević, I. B., Meiselman, H. J.,& Baskurt, O. K.. (2013). The Effect of Alcohols on Red Blood Cell Mechanical Properties and Membrane Fluidity Depends on Their Molecular Size. in Plos One, 8(9), 106-na.
https://hdl.handle.net/21.15107/rcub_ibiss_964

Sonmez M, Ince HY, Yalcin O, Ajdžanović V, Spasojević IB, Meiselman HJ, Baskurt OK. The Effect of Alcohols on Red Blood Cell Mechanical Properties and Membrane Fluidity Depends on Their Molecular Size. in Plos One. 2013;8(9):106-na.
https://hdl.handle.net/21.15107/rcub_ibiss_964 .

Sonmez, Melda, Ince, Huseyin Yavuz, Yalcin, Ozlem, Ajdžanović, Vladimir, Spasojević, Ivan B, Meiselman, Herbert J, Baskurt, Oguz K, "The Effect of Alcohols on Red Blood Cell Mechanical Properties and Membrane Fluidity Depends on Their Molecular Size" in Plos One, 8, no. 9 (2013):106-na,
https://hdl.handle.net/21.15107/rcub_ibiss_964 .

TY  - JOUR
AU  - Ignjatović, Aleksandar
AU  - Stević, Zorica D
AU  - Lavrnić, Dragana S
AU  - Nikolić-Kokić, Aleksandra
AU  - Blagojević, Duško
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan B
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1178
AB  - ALS is characterized by oxidative damage in the brain and cerebrospinal fluid, which is exerted by pro-oxidative activity of iron. Such activity of iron can be drastically increased in the presence of inappropriate iron ligands that catalyze redox cycling of iron, thereby promoting hydroxyl radical generation. The aim of our study was to determine the relative level of inappropriate iron ligands in the cerebrospinal fluid of ALS patients. To determine the levels of inappropriate iron ligands and redox activity of iron in cerebrospinal fluid (10 samples from ALS patients and 10 controls), we applied electron paramagnetic resonance spectroscopy. We have shown that cerebrospinal fluid of ALS patients comprises twofold increased level of inappropriate iron ligands, proportionally increasing iron redox activity and hydroxyl radical production compared to controls. In conclusion, our results strongly support the pro-oxidative/detrimental role of inappropriately chelated iron in ALS pathophysiology. The identification of biomolecules that form such iron complexes and their therapeutic targeting may represent the future of ALS treatment.
T2  - Amyotrophic Lateral Sclerosis
T1  - Inappropriately chelated iron in the cerebrospinal fluid of amyotrophic lateral sclerosis patients
IS  - 4
VL  - 13
EP  - 362
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1178
ER  - 

@article{
author = "Ignjatović, Aleksandar and Stević, Zorica D and Lavrnić, Dragana S and Nikolić-Kokić, Aleksandra and Blagojević, Duško and Spasić, Mihajlo and Spasojević, Ivan B",
year = "2012",
abstract = "ALS is characterized by oxidative damage in the brain and cerebrospinal fluid, which is exerted by pro-oxidative activity of iron. Such activity of iron can be drastically increased in the presence of inappropriate iron ligands that catalyze redox cycling of iron, thereby promoting hydroxyl radical generation. The aim of our study was to determine the relative level of inappropriate iron ligands in the cerebrospinal fluid of ALS patients. To determine the levels of inappropriate iron ligands and redox activity of iron in cerebrospinal fluid (10 samples from ALS patients and 10 controls), we applied electron paramagnetic resonance spectroscopy. We have shown that cerebrospinal fluid of ALS patients comprises twofold increased level of inappropriate iron ligands, proportionally increasing iron redox activity and hydroxyl radical production compared to controls. In conclusion, our results strongly support the pro-oxidative/detrimental role of inappropriately chelated iron in ALS pathophysiology. The identification of biomolecules that form such iron complexes and their therapeutic targeting may represent the future of ALS treatment.",
journal = "Amyotrophic Lateral Sclerosis",
title = "Inappropriately chelated iron in the cerebrospinal fluid of amyotrophic lateral sclerosis patients",
number = "4",
volume = "13",
pages = "362",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1178"
}

Ignjatović, A., Stević, Z. D., Lavrnić, D. S., Nikolić-Kokić, A., Blagojević, D., Spasić, M.,& Spasojević, I. B.. (2012). Inappropriately chelated iron in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. in Amyotrophic Lateral Sclerosis, 13(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1178

Ignjatović A, Stević ZD, Lavrnić DS, Nikolić-Kokić A, Blagojević D, Spasić M, Spasojević IB. Inappropriately chelated iron in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. in Amyotrophic Lateral Sclerosis. 2012;13(4):null-362.
https://hdl.handle.net/21.15107/rcub_ibiss_1178 .

Ignjatović, Aleksandar, Stević, Zorica D, Lavrnić, Dragana S, Nikolić-Kokić, Aleksandra, Blagojević, Duško, Spasić, Mihajlo, Spasojević, Ivan B, "Inappropriately chelated iron in the cerebrospinal fluid of amyotrophic lateral sclerosis patients" in Amyotrophic Lateral Sclerosis, 13, no. 4 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1178 .

TY  - JOUR
AU  - Milošević, Verica
AU  - Ajdžanović, Vladimir
AU  - Spasojević, Ivan B
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1079
AB  - Advanced age is often accompanied by glucocorticoid excess which contributes to the pathogenesis of the metabolic syndrome associated with some hemodynamic disorders. Impaired central regulation of stress hormones secretion and increased glucocorticoids/adrenal androgens ratio trigger hyperglycemia, elevated blood lipids and visceral fat accumulation, associated with hypertension and increased blood viscosity, all of which represent cardiovascular morbidity factors in this age. Finding the adequate therapeutic solutions is set as an imperative in the treatment of listed symptoms. Biologically active soy isoflavones, exhibiting estrogen- and membrane-receptor agonistic/antagonistic activity, and antioxidative and tyrosine kinase/steroidogenic enzyme inhibiting effects, appear as alternative therapeutics for various ageing-related diseases. It has been shown that soy isoflavones reduce some of the listed risk factors, while affecting the hemodynamic group of cardiovascular parameters directly, as well as indirectly via endocrine perturbations. Soy isoflavones may reverse the glucocorticoids/adrenal androgens ratio, lower serum cholesterol, slow the development of atherosclerotic plaque formation, inhibit platelet aggregation, increase cardiac contractility, but they may have diverse effects on blood viscosity and may increase triglyceride levels. Herein, we present the projection of soy isoflavones-based therapy of glucocorticoid excess and disturbed hemodynamics in advanced age, concluding that although promising, it requires the impartial approach and certain precautions.
T2  - General Physiology and Biophysics
T1  - Glucocorticoid excess and disturbed hemodynamics in advanced age: the extent to which soy isoflavones may be beneficial
IS  - 4
VL  - 31
SP  - 3
EP  - 374
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1079
ER  - 

@article{
author = "Milošević, Verica and Ajdžanović, Vladimir and Spasojević, Ivan B",
year = "2012",
abstract = "Advanced age is often accompanied by glucocorticoid excess which contributes to the pathogenesis of the metabolic syndrome associated with some hemodynamic disorders. Impaired central regulation of stress hormones secretion and increased glucocorticoids/adrenal androgens ratio trigger hyperglycemia, elevated blood lipids and visceral fat accumulation, associated with hypertension and increased blood viscosity, all of which represent cardiovascular morbidity factors in this age. Finding the adequate therapeutic solutions is set as an imperative in the treatment of listed symptoms. Biologically active soy isoflavones, exhibiting estrogen- and membrane-receptor agonistic/antagonistic activity, and antioxidative and tyrosine kinase/steroidogenic enzyme inhibiting effects, appear as alternative therapeutics for various ageing-related diseases. It has been shown that soy isoflavones reduce some of the listed risk factors, while affecting the hemodynamic group of cardiovascular parameters directly, as well as indirectly via endocrine perturbations. Soy isoflavones may reverse the glucocorticoids/adrenal androgens ratio, lower serum cholesterol, slow the development of atherosclerotic plaque formation, inhibit platelet aggregation, increase cardiac contractility, but they may have diverse effects on blood viscosity and may increase triglyceride levels. Herein, we present the projection of soy isoflavones-based therapy of glucocorticoid excess and disturbed hemodynamics in advanced age, concluding that although promising, it requires the impartial approach and certain precautions.",
journal = "General Physiology and Biophysics",
title = "Glucocorticoid excess and disturbed hemodynamics in advanced age: the extent to which soy isoflavones may be beneficial",
number = "4",
volume = "31",
pages = "3-374",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1079"
}

Milošević, V., Ajdžanović, V.,& Spasojević, I. B.. (2012). Glucocorticoid excess and disturbed hemodynamics in advanced age: the extent to which soy isoflavones may be beneficial. in General Physiology and Biophysics, 31(4), 3-374.
https://hdl.handle.net/21.15107/rcub_ibiss_1079

Milošević V, Ajdžanović V, Spasojević IB. Glucocorticoid excess and disturbed hemodynamics in advanced age: the extent to which soy isoflavones may be beneficial. in General Physiology and Biophysics. 2012;31(4):3-374.
https://hdl.handle.net/21.15107/rcub_ibiss_1079 .

Milošević, Verica, Ajdžanović, Vladimir, Spasojević, Ivan B, "Glucocorticoid excess and disturbed hemodynamics in advanced age: the extent to which soy isoflavones may be beneficial" in General Physiology and Biophysics, 31, no. 4 (2012):3-374,
https://hdl.handle.net/21.15107/rcub_ibiss_1079 .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Spasojević, Ivan B
AU  - Šošić-Jurjević, Branka 
AU  - Filipović, Branko
AU  - Trifunović, Svetlana
AU  - Sekulić, Milka I.
AU  - Milošević, Verica
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1306
AB  - A decrease of erythrocyte membrane fluidity can contribute to the pathophysiology of hypertension. Soy products, which are used as alternative therapeutics in some cardiovascular conditions, contain various isoflavones (genistein, daidzein, and their glucosides, genistin and daidzin), which can incorporate cellular membrane and change its fluidity. The aim of this study was to examine the effects of soy extract (which generally corresponds to the soy products of isoflavone composition) on erythrocyte membrane fluidity at graded depths. We used electron paramagnetic resonance spectroscopy and fatty acid spin probes (5-DS and 12-DS), the spectra of which are dependent on membrane fluidity. After being treated with soy extract, erythrocytes showed a significant (P = 0.016) decrease of membrane fluidity near the hydrophilic surface, while there were no significant changes of fluidity in deeper hydrophobic membrane regions. These results suggest that soy products containing high levels of genistein and isoflavone glucosides may not be suitable for use in hypertension because they decrease erythrocyte membrane fluidity.
PB  - Springer Nature
T2  - Journal of Membrane Biology
T1  - The Negative Effect of Soy Extract on Erythrocyte Membrane Fluidity: An Electron Paramagnetic Resonance Study
IS  - 3
VL  - 239
DO  - 10.1007/s00232-010-9332-8
SP  - 131
EP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1306
ER  - 

@article{
author = "Ajdžanović, Vladimir and Spasojević, Ivan B and Šošić-Jurjević, Branka  and Filipović, Branko and Trifunović, Svetlana and Sekulić, Milka I. and Milošević, Verica",
year = "2011",
abstract = "A decrease of erythrocyte membrane fluidity can contribute to the pathophysiology of hypertension. Soy products, which are used as alternative therapeutics in some cardiovascular conditions, contain various isoflavones (genistein, daidzein, and their glucosides, genistin and daidzin), which can incorporate cellular membrane and change its fluidity. The aim of this study was to examine the effects of soy extract (which generally corresponds to the soy products of isoflavone composition) on erythrocyte membrane fluidity at graded depths. We used electron paramagnetic resonance spectroscopy and fatty acid spin probes (5-DS and 12-DS), the spectra of which are dependent on membrane fluidity. After being treated with soy extract, erythrocytes showed a significant (P = 0.016) decrease of membrane fluidity near the hydrophilic surface, while there were no significant changes of fluidity in deeper hydrophobic membrane regions. These results suggest that soy products containing high levels of genistein and isoflavone glucosides may not be suitable for use in hypertension because they decrease erythrocyte membrane fluidity.",
publisher = "Springer Nature",
journal = "Journal of Membrane Biology",
title = "The Negative Effect of Soy Extract on Erythrocyte Membrane Fluidity: An Electron Paramagnetic Resonance Study",
number = "3",
volume = "239",
doi = "10.1007/s00232-010-9332-8",
pages = "131-135",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1306"
}

Ajdžanović, V., Spasojević, I. B., Šošić-Jurjević, B., Filipović, B., Trifunović, S., Sekulić, M. I.,& Milošević, V.. (2011). The Negative Effect of Soy Extract on Erythrocyte Membrane Fluidity: An Electron Paramagnetic Resonance Study. in Journal of Membrane Biology
Springer Nature., 239(3), 131-135.
https://doi.org/10.1007/s00232-010-9332-8
https://hdl.handle.net/21.15107/rcub_ibiss_1306

Ajdžanović V, Spasojević IB, Šošić-Jurjević B, Filipović B, Trifunović S, Sekulić MI, Milošević V. The Negative Effect of Soy Extract on Erythrocyte Membrane Fluidity: An Electron Paramagnetic Resonance Study. in Journal of Membrane Biology. 2011;239(3):131-135.
doi:10.1007/s00232-010-9332-8
https://hdl.handle.net/21.15107/rcub_ibiss_1306 .

Ajdžanović, Vladimir, Spasojević, Ivan B, Šošić-Jurjević, Branka , Filipović, Branko, Trifunović, Svetlana, Sekulić, Milka I., Milošević, Verica, "The Negative Effect of Soy Extract on Erythrocyte Membrane Fluidity: An Electron Paramagnetic Resonance Study" in Journal of Membrane Biology, 239, no. 3 (2011):131-135,
https://doi.org/10.1007/s00232-010-9332-8 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1306 .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Spasojević, Ivan B
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Sekulić, Milka I.
AU  - Milošević, Verica
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1389
AB  - The maintenance of erythrocyte membrane fluidity at the physiological level is an important factor affecting the ability of erythrocytes to pass through blood vessels of small luminal diameter. Genistein and daidzein, which are used as alternative therapeutics in cardiovascular conditions, can be incorporated into the cell membrane and change its fluidity. The aim of this study was to examine the effects of genistein and daidzein on erythrocyte membrane fluidity at graded depths. We used electron paramagnetic resonance (EPR) spectroscopy and fatty acid spin probes (5-DS and 12-DS) where EPR spectra were dependent on fluidity. The results showed that genistein significantly (p < 0.05) decreased erythrocyte membrane fluidity near the hydrophilic surface, while daidzein significantly (p < 0.05) increased the same parameter in deeper regions of the membrane. These data suggest that the deep fluidizing effects of daidzein on erythrocyte membranes make it a better therapeutic choice than genistein in some cardiovascular conditions.
PB  - Canadian Science Publishing; National Research Council of Canada
T2  - Canadian Journal of Physiology and Pharmacology
T1  - Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study
IS  - 4
VL  - 88
DO  - 10.1139/Y10-020
SP  - 497
EP  - 500
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1389
ER  - 

@article{
author = "Ajdžanović, Vladimir and Spasojević, Ivan B and Filipović, Branko and Šošić-Jurjević, Branka  and Sekulić, Milka I. and Milošević, Verica",
year = "2010",
abstract = "The maintenance of erythrocyte membrane fluidity at the physiological level is an important factor affecting the ability of erythrocytes to pass through blood vessels of small luminal diameter. Genistein and daidzein, which are used as alternative therapeutics in cardiovascular conditions, can be incorporated into the cell membrane and change its fluidity. The aim of this study was to examine the effects of genistein and daidzein on erythrocyte membrane fluidity at graded depths. We used electron paramagnetic resonance (EPR) spectroscopy and fatty acid spin probes (5-DS and 12-DS) where EPR spectra were dependent on fluidity. The results showed that genistein significantly (p < 0.05) decreased erythrocyte membrane fluidity near the hydrophilic surface, while daidzein significantly (p < 0.05) increased the same parameter in deeper regions of the membrane. These data suggest that the deep fluidizing effects of daidzein on erythrocyte membranes make it a better therapeutic choice than genistein in some cardiovascular conditions.",
publisher = "Canadian Science Publishing; National Research Council of Canada",
journal = "Canadian Journal of Physiology and Pharmacology",
title = "Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study",
number = "4",
volume = "88",
doi = "10.1139/Y10-020",
pages = "497-500",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1389"
}

Ajdžanović, V., Spasojević, I. B., Filipović, B., Šošić-Jurjević, B., Sekulić, M. I.,& Milošević, V.. (2010). Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study. in Canadian Journal of Physiology and Pharmacology
Canadian Science Publishing; National Research Council of Canada., 88(4), 497-500.
https://doi.org/10.1139/Y10-020
https://hdl.handle.net/21.15107/rcub_ibiss_1389

Ajdžanović V, Spasojević IB, Filipović B, Šošić-Jurjević B, Sekulić MI, Milošević V. Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study. in Canadian Journal of Physiology and Pharmacology. 2010;88(4):497-500.
doi:10.1139/Y10-020
https://hdl.handle.net/21.15107/rcub_ibiss_1389 .

Ajdžanović, Vladimir, Spasojević, Ivan B, Filipović, Branko, Šošić-Jurjević, Branka , Sekulić, Milka I., Milošević, Verica, "Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study" in Canadian Journal of Physiology and Pharmacology, 88, no. 4 (2010):497-500,
https://doi.org/10.1139/Y10-020 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1389 .

TY  - JOUR
AU  - Spasojević, Ivan B
AU  - Morina, Arian
AU  - Mojović, Milos D
AU  - Stević, Zorica D
AU  - Spasić, Snezana D
AU  - Jones, David R
AU  - Spasić, Mihajlo
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1405
AB  - A breakdown in homeostasis of redox-active metals represents an important factor for neurodegeneration. We have used EPR spectroscopy and BMPO spin-trap to investigate the catalytic properties and ligand modulation of redox activity of copper and iron in human cerebrospinal fluid (CSF). In contrast to iron, copper supplementation provoked a statistically significant increase in hydroxyl free radical generation in CSF treated with H(2)O(2). However, in a binary copper/iron containing Fenton system, iron catalytically activated copper. The chelator EDTA, which represents a model of physiological metal ligands, completely prevented copper's redox activity in CSF, while iron chelation led to a significant increase in hydroxyl radical generation, indicating that copper and iron do not only have diverse catalytic properties in the CSF but also that their redox activities are differently modulated by ligands. The application of DDC reduced hydroxyl radical generation in the CSF containing catalytically active metals (free Cu(2+) or Fe(3+)-EDTA complex). We conclude that chelators, such as DDC, are capable of preventing the pro-oxidative activity of both metals and may be suitable for reducing hydroxyl radical formation in certain pathophysiological settings.
T2  - Redox Report
T1  - Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid
IS  - 1
VL  - 15
EP  - 35
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1405
ER  - 

@article{
author = "Spasojević, Ivan B and Morina, Arian and Mojović, Milos D and Stević, Zorica D and Spasić, Snezana D and Jones, David R and Spasić, Mihajlo",
year = "2010",
abstract = "A breakdown in homeostasis of redox-active metals represents an important factor for neurodegeneration. We have used EPR spectroscopy and BMPO spin-trap to investigate the catalytic properties and ligand modulation of redox activity of copper and iron in human cerebrospinal fluid (CSF). In contrast to iron, copper supplementation provoked a statistically significant increase in hydroxyl free radical generation in CSF treated with H(2)O(2). However, in a binary copper/iron containing Fenton system, iron catalytically activated copper. The chelator EDTA, which represents a model of physiological metal ligands, completely prevented copper's redox activity in CSF, while iron chelation led to a significant increase in hydroxyl radical generation, indicating that copper and iron do not only have diverse catalytic properties in the CSF but also that their redox activities are differently modulated by ligands. The application of DDC reduced hydroxyl radical generation in the CSF containing catalytically active metals (free Cu(2+) or Fe(3+)-EDTA complex). We conclude that chelators, such as DDC, are capable of preventing the pro-oxidative activity of both metals and may be suitable for reducing hydroxyl radical formation in certain pathophysiological settings.",
journal = "Redox Report",
title = "Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid",
number = "1",
volume = "15",
pages = "35",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1405"
}

Spasojević, I. B., Morina, A., Mojović, M. D., Stević, Z. D., Spasić, S. D., Jones, D. R.,& Spasić, M.. (2010). Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid. in Redox Report, 15(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1405

Spasojević IB, Morina A, Mojović MD, Stević ZD, Spasić SD, Jones DR, Spasić M. Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid. in Redox Report. 2010;15(1):null-35.
https://hdl.handle.net/21.15107/rcub_ibiss_1405 .

Spasojević, Ivan B, Morina, Arian, Mojović, Milos D, Stević, Zorica D, Spasić, Snezana D, Jones, David R, Spasić, Mihajlo, "Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid" in Redox Report, 15, no. 1 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1405 .

TY  - JOUR
AU  - Spasojević, Ivan B
AU  - Stević, Zorica D
AU  - Nikolić-Kokić, Aleksandra
AU  - Jones, David R
AU  - Blagojević, Duško
AU  - Spasić, Mihajlo
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1392
AB  - Ferrous iron, released from iron deposits in the motor cortex and other brain regions of amyotrophic lateral sclerosis (ALS) patients, participates in the Fenton reaction in cerebrospinal fluid (CSF) alongside H(2)O(2), which is continuously released by neuronal cells. In vivo, the production of notoriously reactive hydroxyl radicals via this reaction could lead to the progression of the disease. Herein, we have examined the effect of ascorbate and uric acid on the production of hydroxyl radicals in CSF from both sporadic ALS patients and control subjects. Electron paramagnetic resonance spectroscopy identified ascorbyl radicals in CSF from ALS patients whereas it was undetectable in control CSF. The addition of H(2)O(2) to the CSF from ALS patients provoked further formation of ascorbyl radicals and the formation of hydroxyl radicals ex vivo. The hydroxyl addition of uric acid to CSF from ALS patients diminished the production of hydroxyl radicals. In conclusion, there are clear differences between the roles of the two examined radical scavengers in the CSF of ALS patients indicating that the use of ascorbate could have unfavourable effects in ALS patients.
T2  - Redox Report
T1  - Different roles of radical scavengers - ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients
IS  - 2
VL  - 15
EP  - 86
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1392
ER  - 

@article{
author = "Spasojević, Ivan B and Stević, Zorica D and Nikolić-Kokić, Aleksandra and Jones, David R and Blagojević, Duško and Spasić, Mihajlo",
year = "2010",
abstract = "Ferrous iron, released from iron deposits in the motor cortex and other brain regions of amyotrophic lateral sclerosis (ALS) patients, participates in the Fenton reaction in cerebrospinal fluid (CSF) alongside H(2)O(2), which is continuously released by neuronal cells. In vivo, the production of notoriously reactive hydroxyl radicals via this reaction could lead to the progression of the disease. Herein, we have examined the effect of ascorbate and uric acid on the production of hydroxyl radicals in CSF from both sporadic ALS patients and control subjects. Electron paramagnetic resonance spectroscopy identified ascorbyl radicals in CSF from ALS patients whereas it was undetectable in control CSF. The addition of H(2)O(2) to the CSF from ALS patients provoked further formation of ascorbyl radicals and the formation of hydroxyl radicals ex vivo. The hydroxyl addition of uric acid to CSF from ALS patients diminished the production of hydroxyl radicals. In conclusion, there are clear differences between the roles of the two examined radical scavengers in the CSF of ALS patients indicating that the use of ascorbate could have unfavourable effects in ALS patients.",
journal = "Redox Report",
title = "Different roles of radical scavengers - ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients",
number = "2",
volume = "15",
pages = "86",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1392"
}

Spasojević, I. B., Stević, Z. D., Nikolić-Kokić, A., Jones, D. R., Blagojević, D.,& Spasić, M.. (2010). Different roles of radical scavengers - ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. in Redox Report, 15(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1392

Spasojević IB, Stević ZD, Nikolić-Kokić A, Jones DR, Blagojević D, Spasić M. Different roles of radical scavengers - ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. in Redox Report. 2010;15(2):null-86.
https://hdl.handle.net/21.15107/rcub_ibiss_1392 .

Spasojević, Ivan B, Stević, Zorica D, Nikolić-Kokić, Aleksandra, Jones, David R, Blagojević, Duško, Spasić, Mihajlo, "Different roles of radical scavengers - ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients" in Redox Report, 15, no. 2 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1392 .

TY  - JOUR
AU  - Kojić, Danijela K
AU  - Spasojević, Ivan B
AU  - Mojović, Milos D
AU  - Blagojević, Duško
AU  - Worland, Roger M
AU  - Grubor-Lajšić, Gordana
AU  - Spasić, Mihajlo
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1471
AB  - The aim of this study was to investigate the relationship between antioxidant enzymes and reactive oxygen species production in diapausing larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Pyralidae) kept at 5 degrees C, -3 degrees C and -16 degrees C for two weeks. The amount of hydrogen peroxide (H(2)O(2)), activity of antioxidant enzymes, copper zinc superoxide dismutase (CuZnSOD), manganese superoxide dismutases (MnSOD) and catalase (CAT) in whole body homogenates, as well as the electron paramagnetic resonance (EPR) spectroscopy of this insect's whole body were analysed. A higher level of melanin radical and lower CuZnSOD and CAT activities were found in larvae kept at -3 degrees C than at 5 degrees C and -16 degrees C. At the same temperature (-3 degrees C) an elevated H(2)O(2) concentration was recorded. A possible regulatory role of H(2)O(2) at -3 degrees C, which is the temperature that triggers freezing tolerance, is suggested.
T2  - European Journal of Entomology
T1  - Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae)
IS  - 3
VL  - 106
EP  - 454
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1471
ER  - 

@article{
author = "Kojić, Danijela K and Spasojević, Ivan B and Mojović, Milos D and Blagojević, Duško and Worland, Roger M and Grubor-Lajšić, Gordana and Spasić, Mihajlo",
year = "2009",
abstract = "The aim of this study was to investigate the relationship between antioxidant enzymes and reactive oxygen species production in diapausing larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Pyralidae) kept at 5 degrees C, -3 degrees C and -16 degrees C for two weeks. The amount of hydrogen peroxide (H(2)O(2)), activity of antioxidant enzymes, copper zinc superoxide dismutase (CuZnSOD), manganese superoxide dismutases (MnSOD) and catalase (CAT) in whole body homogenates, as well as the electron paramagnetic resonance (EPR) spectroscopy of this insect's whole body were analysed. A higher level of melanin radical and lower CuZnSOD and CAT activities were found in larvae kept at -3 degrees C than at 5 degrees C and -16 degrees C. At the same temperature (-3 degrees C) an elevated H(2)O(2) concentration was recorded. A possible regulatory role of H(2)O(2) at -3 degrees C, which is the temperature that triggers freezing tolerance, is suggested.",
journal = "European Journal of Entomology",
title = "Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae)",
number = "3",
volume = "106",
pages = "454",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1471"
}

Kojić, D. K., Spasojević, I. B., Mojović, M. D., Blagojević, D., Worland, R. M., Grubor-Lajšić, G.,& Spasić, M.. (2009). Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae). in European Journal of Entomology, 106(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1471

Kojić DK, Spasojević IB, Mojović MD, Blagojević D, Worland RM, Grubor-Lajšić G, Spasić M. Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae). in European Journal of Entomology. 2009;106(3):null-454.
https://hdl.handle.net/21.15107/rcub_ibiss_1471 .

Kojić, Danijela K, Spasojević, Ivan B, Mojović, Milos D, Blagojević, Duško, Worland, Roger M, Grubor-Lajšić, Gordana, Spasić, Mihajlo, "Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae)" in European Journal of Entomology, 106, no. 3 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1471 .

TY  - JOUR
AU  - Milovanović, Slobodan R
AU  - Oreščanin Dušić, Zorana
AU  - Blagojević, Duško
AU  - Nikolić-Kokić, Aleksandra
AU  - Radojičić, Ratko M.
AU  - Spasojević, Ivan B
AU  - Spasić, Mihajlo
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1457
AB  - Protamine sulphate causes potassium ion channel-mediated relaxation of spontaneous and calcium ion-induced contractions of the isolated rat uterus. Diethyldithiocarbamate (DDC) potentiated the effect of protamine sulphate. A mechanism for DDC's action was postulated on the basis of its interactions with divalent iron ions and Cui Zn-SOD. DDC chelates divalent iron ions creating DDC-iron (Fe-DDC) complexes. Fe-DDC forms stable NO-Fe-DDC(2) complexes by NO scavenging and de-nitrosylation processes, which in combination with DDC (5 mM) provoke inhibition of Cui Zn-SOD resulting in specific oxidative conditions culminating in potassium ion channel opening, membrane hyperpolarisation, inhibition of calcium ion influx and subsequent muscle relaxation. As Fe-DDC and NO-Fe-DDC(2) complexes exclude divalent iron ions from participating in the hydroxy radical generating Fenton reaction, DDC can also prevent iron-related pathophysiological manifestations. Such permissive roles of DDC open the possibility for application of its pharmacological form (disulfiram) to a wider spectrum of pathophysiological conditions related to smooth muscles.
T2  - Redox Report
T1  - Diethyldithiocarbamate potentiates the effects of protamine sulphate in the isolated rat uterus
IS  - 2
VL  - 14
EP  - 54
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1457
ER  - 

@article{
author = "Milovanović, Slobodan R and Oreščanin Dušić, Zorana and Blagojević, Duško and Nikolić-Kokić, Aleksandra and Radojičić, Ratko M. and Spasojević, Ivan B and Spasić, Mihajlo",
year = "2009",
abstract = "Protamine sulphate causes potassium ion channel-mediated relaxation of spontaneous and calcium ion-induced contractions of the isolated rat uterus. Diethyldithiocarbamate (DDC) potentiated the effect of protamine sulphate. A mechanism for DDC's action was postulated on the basis of its interactions with divalent iron ions and Cui Zn-SOD. DDC chelates divalent iron ions creating DDC-iron (Fe-DDC) complexes. Fe-DDC forms stable NO-Fe-DDC(2) complexes by NO scavenging and de-nitrosylation processes, which in combination with DDC (5 mM) provoke inhibition of Cui Zn-SOD resulting in specific oxidative conditions culminating in potassium ion channel opening, membrane hyperpolarisation, inhibition of calcium ion influx and subsequent muscle relaxation. As Fe-DDC and NO-Fe-DDC(2) complexes exclude divalent iron ions from participating in the hydroxy radical generating Fenton reaction, DDC can also prevent iron-related pathophysiological manifestations. Such permissive roles of DDC open the possibility for application of its pharmacological form (disulfiram) to a wider spectrum of pathophysiological conditions related to smooth muscles.",
journal = "Redox Report",
title = "Diethyldithiocarbamate potentiates the effects of protamine sulphate in the isolated rat uterus",
number = "2",
volume = "14",
pages = "54",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1457"
}

Milovanović, S. R., Oreščanin Dušić, Z., Blagojević, D., Nikolić-Kokić, A., Radojičić, R. M., Spasojević, I. B.,& Spasić, M.. (2009). Diethyldithiocarbamate potentiates the effects of protamine sulphate in the isolated rat uterus. in Redox Report, 14(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1457

Milovanović SR, Oreščanin Dušić Z, Blagojević D, Nikolić-Kokić A, Radojičić RM, Spasojević IB, Spasić M. Diethyldithiocarbamate potentiates the effects of protamine sulphate in the isolated rat uterus. in Redox Report. 2009;14(2):null-54.
https://hdl.handle.net/21.15107/rcub_ibiss_1457 .

Milovanović, Slobodan R, Oreščanin Dušić, Zorana, Blagojević, Duško, Nikolić-Kokić, Aleksandra, Radojičić, Ratko M., Spasojević, Ivan B, Spasić, Mihajlo, "Diethyldithiocarbamate potentiates the effects of protamine sulphate in the isolated rat uterus" in Redox Report, 14, no. 2 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1457 .

TY  - CONF
AU  - Mojović, Milos D
AU  - Spasojević, Ivan B
AU  - Spasić, Mihajlo
AU  - Bacić, Goran G
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1665
C3  - Free Radical Research
T1  - Fenton reaction produces hydrogen radical (center dot H) in chemical and biological systems
IS  - null
VL  - 40
EP  - S72
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1665
ER  - 

@conference{
author = "Mojović, Milos D and Spasojević, Ivan B and Spasić, Mihajlo and Bacić, Goran G",
year = "2006",
journal = "Free Radical Research",
title = "Fenton reaction produces hydrogen radical (center dot H) in chemical and biological systems",
number = "null",
volume = "40",
pages = "S72",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1665"
}

Mojović, M. D., Spasojević, I. B., Spasić, M.,& Bacić, G. G.. (2006). Fenton reaction produces hydrogen radical (center dot H) in chemical and biological systems. in Free Radical Research, 40(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1665

Mojović MD, Spasojević IB, Spasić M, Bacić GG. Fenton reaction produces hydrogen radical (center dot H) in chemical and biological systems. in Free Radical Research. 2006;40(null):null-S72.
https://hdl.handle.net/21.15107/rcub_ibiss_1665 .

Mojović, Milos D, Spasojević, Ivan B, Spasić, Mihajlo, Bacić, Goran G, "Fenton reaction produces hydrogen radical (center dot H) in chemical and biological systems" in Free Radical Research, 40, no. null (2006),
https://hdl.handle.net/21.15107/rcub_ibiss_1665 .

TY  - CONF
AU  - Spasojević, Ivan B
AU  - Mojović, Milos D
AU  - Stević, Zorica D
AU  - Batas, Valentina
AU  - Bacić, Goran G
AU  - Spasić, Mihajlo
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1666
C3  - Free Radical Research
T1  - Capacity of cerebrospinal fluid to transform hydrogen peroxide-relation to neurodegenerative changes in ALS
IS  - null
VL  - 40
EP  - S90
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1666
ER  - 

@conference{
author = "Spasojević, Ivan B and Mojović, Milos D and Stević, Zorica D and Batas, Valentina and Bacić, Goran G and Spasić, Mihajlo",
year = "2006",
journal = "Free Radical Research",
title = "Capacity of cerebrospinal fluid to transform hydrogen peroxide-relation to neurodegenerative changes in ALS",
number = "null",
volume = "40",
pages = "S90",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1666"
}

Spasojević, I. B., Mojović, M. D., Stević, Z. D., Batas, V., Bacić, G. G.,& Spasić, M.. (2006). Capacity of cerebrospinal fluid to transform hydrogen peroxide-relation to neurodegenerative changes in ALS. in Free Radical Research, 40(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1666

Spasojević IB, Mojović MD, Stević ZD, Batas V, Bacić GG, Spasić M. Capacity of cerebrospinal fluid to transform hydrogen peroxide-relation to neurodegenerative changes in ALS. in Free Radical Research. 2006;40(null):null-S90.
https://hdl.handle.net/21.15107/rcub_ibiss_1666 .

Spasojević, Ivan B, Mojović, Milos D, Stević, Zorica D, Batas, Valentina, Bacić, Goran G, Spasić, Mihajlo, "Capacity of cerebrospinal fluid to transform hydrogen peroxide-relation to neurodegenerative changes in ALS" in Free Radical Research, 40, no. null (2006),
https://hdl.handle.net/21.15107/rcub_ibiss_1666 .