TY  - CONF
AU  - Smailagić, Dijana
AU  - Dragišić Maksimović, Jelena
AU  - Marin, Marija
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Stupar, Sofija
AU  - Stanišić, Mariana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6710
AB  - Phloretin is a phenolic compound from the apple that belongs to the group of dihydrochalcones. The genus Malus is a uniquely rich source of dihydrochalcones, accounting for about 97% of the total phenolic compounds in apple leaves and 10-20% of the dry weight of the leaf tissue. Due to its typical dihydrochalcone structure, phloretin is a highly reactive molecule that interacts efficiently with many biological macromolecules. Its reactivity leads to remarkable antioxidant and medicinal properties that have been extensively studied in mammals and humans. However, knowledge about the effects of phloretin on plant organisms is rather scarce. The phytotoxicity of phloretin in plants was first documented by our research group when phloretin was recognised as a potent allelochemical of the apple tree with deleterious effects in plant species outside the genus Malus. Research on the model plant Arabidopsis [Arabidopsis thaliana (L.) Heynh] named the disruption of auxin homeostasis in the roots as the main mechanism of the phytotoxic effect of phloretin. The present study complements previous research with new insights into the ultrastructural changes in leaf mesophyll cells that underlie the growth arrest and decay of the aerial parts of Arabidopsis seedlings exposed to phloretin. The effects of phloretin are closely linked to the ultrastructural damage in the leaf mesophyll cells and range from mild effects with short-term treatment to severe effects with long-term treatment. Transmission electron microscopy revealed changes in the morphology of the cell wall, changes in thylakoid organisation, swelling and displacement of chloroplasts after short-term treatment. A significant decrease in the number of starch granules and chlorophyll content in the leaves reflected impaired chloroplast function leading to depletion of starch reserves, starvation of cells and recruitment of micro- and macroautophagic processes for self-maintenance leading to programmed cell death after long-term treatment with phloretin. These results confirm that the sites of action of phloretin in plant and animal cells do not coincide, confirming the safety of its potential application as a new bioherbicidal agent for humans and animals.
PB  - Turkey. Gebze,Turkey: Technical University
C3  - Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey
T1  - How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?
SP  - ID-739
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6710
ER  - 

@conference{
author = "Smailagić, Dijana and Dragišić Maksimović, Jelena and Marin, Marija and Banjac, Nevena and Ninković, Slavica and Stupar, Sofija and Stanišić, Mariana",
year = "2024",
abstract = "Phloretin is a phenolic compound from the apple that belongs to the group of dihydrochalcones. The genus Malus is a uniquely rich source of dihydrochalcones, accounting for about 97% of the total phenolic compounds in apple leaves and 10-20% of the dry weight of the leaf tissue. Due to its typical dihydrochalcone structure, phloretin is a highly reactive molecule that interacts efficiently with many biological macromolecules. Its reactivity leads to remarkable antioxidant and medicinal properties that have been extensively studied in mammals and humans. However, knowledge about the effects of phloretin on plant organisms is rather scarce. The phytotoxicity of phloretin in plants was first documented by our research group when phloretin was recognised as a potent allelochemical of the apple tree with deleterious effects in plant species outside the genus Malus. Research on the model plant Arabidopsis [Arabidopsis thaliana (L.) Heynh] named the disruption of auxin homeostasis in the roots as the main mechanism of the phytotoxic effect of phloretin. The present study complements previous research with new insights into the ultrastructural changes in leaf mesophyll cells that underlie the growth arrest and decay of the aerial parts of Arabidopsis seedlings exposed to phloretin. The effects of phloretin are closely linked to the ultrastructural damage in the leaf mesophyll cells and range from mild effects with short-term treatment to severe effects with long-term treatment. Transmission electron microscopy revealed changes in the morphology of the cell wall, changes in thylakoid organisation, swelling and displacement of chloroplasts after short-term treatment. A significant decrease in the number of starch granules and chlorophyll content in the leaves reflected impaired chloroplast function leading to depletion of starch reserves, starvation of cells and recruitment of micro- and macroautophagic processes for self-maintenance leading to programmed cell death after long-term treatment with phloretin. These results confirm that the sites of action of phloretin in plant and animal cells do not coincide, confirming the safety of its potential application as a new bioherbicidal agent for humans and animals.",
publisher = "Turkey. Gebze,Turkey: Technical University",
journal = "Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey",
title = "How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?",
pages = "ID-739",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6710"
}

Smailagić, D., Dragišić Maksimović, J., Marin, M., Banjac, N., Ninković, S., Stupar, S.,& Stanišić, M.. (2024). How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?. in Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey
Turkey. Gebze,Turkey: Technical University., ID-739.
https://hdl.handle.net/21.15107/rcub_ibiss_6710

Smailagić D, Dragišić Maksimović J, Marin M, Banjac N, Ninković S, Stupar S, Stanišić M. How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?. in Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey. 2024;:ID-739.
https://hdl.handle.net/21.15107/rcub_ibiss_6710 .

Smailagić, Dijana, Dragišić Maksimović, Jelena, Marin, Marija, Banjac, Nevena, Ninković, Slavica, Stupar, Sofija, Stanišić, Mariana, "How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?" in Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey (2024):ID-739,
https://hdl.handle.net/21.15107/rcub_ibiss_6710 .

TY  - JOUR
AU  - Pokimica, Nina
AU  - Ćosić, Tatjana
AU  - Uzelac, Branka
AU  - Ninković, Slavica
AU  - Raspor, Martin
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6631
AB  - Numerous biotechnological applications require a fast and efficient clonal propagation of whole plants under controlled laboratory conditions. For most plant species, the de novo regeneration of shoots from the cuttings of various plant organs can be obtained on nutrient media supplemented with plant hormones, auxin and cytokinin. While auxin is needed during the early stages of the process that include the establishment of pluripotent primordia and the subsequent acquisition of organogenic competence, cytokinin-supplemented media are required to induce these primordia to differentiate into developing shoots. The perception of cytokinin through the receptor ARABIDOPSIS HISTIDINE KINASE4 (AHK4) is crucial for the activation of the two main regulators of the establishment and maintenance of shoot apical meristems (SAMs): SHOOTMERISTEMLESS (STM) and the WUSCHEL-CLAVATA3 (WUS-CLV3) regulatory circuit. In this review, we summarize the current knowledge of the roles of the cytokinin signaling cascade in the perception and transduction of signals that are crucial for the de novo establishment of SAMs and lead to the desired biotechnological output—adventitious shoot multiplication. We highlight the functional differences between individual members of the multigene families involved in cytokinin signal transduction, and demonstrate how complex genetic regulation can be achieved through functional specialization of individual gene family members.
PB  - Basel: MDPI
T2  - Biomolecules
T1  - Dissecting the Roles of the Cytokinin Signaling Network: The Case of De Novo Shoot Apical Meristem Formation
IS  - 3
VL  - 14
DO  - 10.3390/biom14030381
SP  - 381
ER  - 

@article{
author = "Pokimica, Nina and Ćosić, Tatjana and Uzelac, Branka and Ninković, Slavica and Raspor, Martin",
year = "2024",
abstract = "Numerous biotechnological applications require a fast and efficient clonal propagation of whole plants under controlled laboratory conditions. For most plant species, the de novo regeneration of shoots from the cuttings of various plant organs can be obtained on nutrient media supplemented with plant hormones, auxin and cytokinin. While auxin is needed during the early stages of the process that include the establishment of pluripotent primordia and the subsequent acquisition of organogenic competence, cytokinin-supplemented media are required to induce these primordia to differentiate into developing shoots. The perception of cytokinin through the receptor ARABIDOPSIS HISTIDINE KINASE4 (AHK4) is crucial for the activation of the two main regulators of the establishment and maintenance of shoot apical meristems (SAMs): SHOOTMERISTEMLESS (STM) and the WUSCHEL-CLAVATA3 (WUS-CLV3) regulatory circuit. In this review, we summarize the current knowledge of the roles of the cytokinin signaling cascade in the perception and transduction of signals that are crucial for the de novo establishment of SAMs and lead to the desired biotechnological output—adventitious shoot multiplication. We highlight the functional differences between individual members of the multigene families involved in cytokinin signal transduction, and demonstrate how complex genetic regulation can be achieved through functional specialization of individual gene family members.",
publisher = "Basel: MDPI",
journal = "Biomolecules",
title = "Dissecting the Roles of the Cytokinin Signaling Network: The Case of De Novo Shoot Apical Meristem Formation",
number = "3",
volume = "14",
doi = "10.3390/biom14030381",
pages = "381"
}

Pokimica, N., Ćosić, T., Uzelac, B., Ninković, S.,& Raspor, M.. (2024). Dissecting the Roles of the Cytokinin Signaling Network: The Case of De Novo Shoot Apical Meristem Formation. in Biomolecules
Basel: MDPI., 14(3), 381.
https://doi.org/10.3390/biom14030381

Pokimica N, Ćosić T, Uzelac B, Ninković S, Raspor M. Dissecting the Roles of the Cytokinin Signaling Network: The Case of De Novo Shoot Apical Meristem Formation. in Biomolecules. 2024;14(3):381.
doi:10.3390/biom14030381 .

Pokimica, Nina, Ćosić, Tatjana, Uzelac, Branka, Ninković, Slavica, Raspor, Martin, "Dissecting the Roles of the Cytokinin Signaling Network: The Case of De Novo Shoot Apical Meristem Formation" in Biomolecules, 14, no. 3 (2024):381,
https://doi.org/10.3390/biom14030381 . .

TY  - CONF
AU  - Ćosić, Tatjana
AU  - Anđela, Tomić
AU  - Uzelac, Branka
AU  - Raspor, Martin
AU  - Pokimica, Nina
AU  - Devrnja, Nina
AU  - Ninković, Slavica
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6111
AB  - Deposition of carbohydrates in the lower part of the stem represents a unique feature of kohlrabi (Brassica
oleracea var. gongylodes), a member of Brassicaceae family grown for its high nutritional value. Over the
past decade our team intensely investigated in vitro development and regeneration of this significant crop,
determining, among other specific characteristics, an intricate relation between sucrose, serving as a carbon
source for in vitro growth, and cytokinins (CKs), utilized for the induction of indirect de novo shoot
organogenesis (DNSO). Even though the formation of kohlrabi stem tuber in vitro has not yet been
successfully attained, development of an efficient in vitro system for kohlrabi growth and DNSO opened the
possibility to study sugar metabolism in young kohlrabi plants, including the effects of elevated sucrose
application (6% and 9%) and CKs (trans-zeatin, transZ; thidiazuron, TDZ). Expression analysis of ten
selected genes involved in sugar partitioning in plants, indicated that both sucrose and CKs had similar
influence in the sense of downregulating these genes compared to control grown on plant growth
regulator-free medium with 3% sucrose. TDZ application had the highest effect on relative expression of all
three sucrose synthase genes (SUS1/3/6), particularly in three week-old plantlets (S1), the developmental
stage characterized by callus formation in the CK-treated kohlrabi. This trend was also observed for the
CWINV1, coding for β-fructofuranosidase and UGP1, coding for UTP--glucose-1-phosphate uridylyltransferase
1. On the other hand, high concentration of sucrose induced stronger downregulation of CINV1 (alkaline/
neutral invertase1), HXK1 (hexokinase1), GPI (glucose-6-phosphate isomerase) and SPS1 (sucrose-phosphate
synthase 1). TransZ led to small changes in gene expression, and even led to upregulation of SUS1 and
CINV1. Older plantlets (S2, four week-old, forming de novo shoots on CK treatment) were generally less
affected by applied treatments. In addition, histological sections of treated kohlrabi hypocotyls revealed
distinct changes at morpho-anatomical level. Our results, for the first time, depict effects of sucrose and CK
on activity of soluble sugars-related gene in young kohlrabi, suggesting an altered metabolism and transport
of soluble sugars, providing a starting point to the elucidation of stem tuber development and sugar
partitioning in kohlrabi.
PB  - Daejeon: International Association For Plant Biotechnology
C3  - Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea
T1  - Sucrose and cytokinins alter sugar metabolism in young in vitro grown kohlrabi
SP  - 345
EP  - 345
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6111
ER  - 

@conference{
author = "Ćosić, Tatjana and Anđela, Tomić and Uzelac, Branka and Raspor, Martin and Pokimica, Nina and Devrnja, Nina and Ninković, Slavica",
year = "2023",
abstract = "Deposition of carbohydrates in the lower part of the stem represents a unique feature of kohlrabi (Brassica
oleracea var. gongylodes), a member of Brassicaceae family grown for its high nutritional value. Over the
past decade our team intensely investigated in vitro development and regeneration of this significant crop,
determining, among other specific characteristics, an intricate relation between sucrose, serving as a carbon
source for in vitro growth, and cytokinins (CKs), utilized for the induction of indirect de novo shoot
organogenesis (DNSO). Even though the formation of kohlrabi stem tuber in vitro has not yet been
successfully attained, development of an efficient in vitro system for kohlrabi growth and DNSO opened the
possibility to study sugar metabolism in young kohlrabi plants, including the effects of elevated sucrose
application (6% and 9%) and CKs (trans-zeatin, transZ; thidiazuron, TDZ). Expression analysis of ten
selected genes involved in sugar partitioning in plants, indicated that both sucrose and CKs had similar
influence in the sense of downregulating these genes compared to control grown on plant growth
regulator-free medium with 3% sucrose. TDZ application had the highest effect on relative expression of all
three sucrose synthase genes (SUS1/3/6), particularly in three week-old plantlets (S1), the developmental
stage characterized by callus formation in the CK-treated kohlrabi. This trend was also observed for the
CWINV1, coding for β-fructofuranosidase and UGP1, coding for UTP--glucose-1-phosphate uridylyltransferase
1. On the other hand, high concentration of sucrose induced stronger downregulation of CINV1 (alkaline/
neutral invertase1), HXK1 (hexokinase1), GPI (glucose-6-phosphate isomerase) and SPS1 (sucrose-phosphate
synthase 1). TransZ led to small changes in gene expression, and even led to upregulation of SUS1 and
CINV1. Older plantlets (S2, four week-old, forming de novo shoots on CK treatment) were generally less
affected by applied treatments. In addition, histological sections of treated kohlrabi hypocotyls revealed
distinct changes at morpho-anatomical level. Our results, for the first time, depict effects of sucrose and CK
on activity of soluble sugars-related gene in young kohlrabi, suggesting an altered metabolism and transport
of soluble sugars, providing a starting point to the elucidation of stem tuber development and sugar
partitioning in kohlrabi.",
publisher = "Daejeon: International Association For Plant Biotechnology",
journal = "Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea",
title = "Sucrose and cytokinins alter sugar metabolism in young in vitro grown kohlrabi",
pages = "345-345",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6111"
}

Ćosić, T., Anđela, T., Uzelac, B., Raspor, M., Pokimica, N., Devrnja, N.,& Ninković, S.. (2023). Sucrose and cytokinins alter sugar metabolism in young in vitro grown kohlrabi. in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea
Daejeon: International Association For Plant Biotechnology., 345-345.
https://hdl.handle.net/21.15107/rcub_ibiss_6111

Ćosić T, Anđela T, Uzelac B, Raspor M, Pokimica N, Devrnja N, Ninković S. Sucrose and cytokinins alter sugar metabolism in young in vitro grown kohlrabi. in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea. 2023;:345-345.
https://hdl.handle.net/21.15107/rcub_ibiss_6111 .

Ćosić, Tatjana, Anđela, Tomić, Uzelac, Branka, Raspor, Martin, Pokimica, Nina, Devrnja, Nina, Ninković, Slavica, "Sucrose and cytokinins alter sugar metabolism in young in vitro grown kohlrabi" in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea (2023):345-345,
https://hdl.handle.net/21.15107/rcub_ibiss_6111 .

TY  - CONF
AU  - Ninković, Slavica
AU  - Banjac, Nevena
AU  - Stanišić, Mariana
AU  - Smailagić, Dijana
AU  - Ćosić, Tatjana
AU  - Živanović, Branka
AU  - Dobrev, Petre I.
AU  - Motyka, Vaclav
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6112
AB  - Root-exuded compounds play a key role in the regulation of plant allelopathic interactions. In contrast to
numerous studies showing that many secondary metabolites have an allelopathic function, evidence of
exudation of phytohormones into the rhizosphere and their involvement in allelopathic interactions is scarce.
We postulated that phytohormones secreted by roots into the environment may act as allelochemicals that
affect the growth performance of neighboring plants. Hairy root culture media of two distinct species, the fruit
tree species Malus domestica cv. Golden delicious[1] and the herbaceous weed species Chenopodium murale
[2,3] were collected and analyzed by ultra-high-performance liquid chromatography coupled with tandem mass
spectrometry (UHPLC-MS/MS). We found that most of plant growth substances exuded by the hairy roots of
C. murale and M. domestica were assorted from fraction A (96.8% and even 98.9%, respectively) including
OxIAA, PAA, SA, BzA, and ABA, with SA and BzA being the most abundant, whereas those from fraction B,
including cytokinins and the ethylene precursor ACC, accounted for only 1% of the regulatory substances
detected in both species. Exogenous application of SA at concentrations released by the hairy roots of the
respective plant species and accumulated in the culture media for four weeks significantly affected the hairy
root growth of the other species and also the shoot and root growth of the Arabidopsis seedlings. The
disruptive effect of SA in the exuded concentration on the membrane potential of Arabidopsis root cells was
determined. The data obtained would be useful for designing further studies aimed at elucidating the
contribution and role of exuded phytohormones to overall allelopathic potential of these two plant species.
OxIAA = 2-oxindole-3-acetic acid, PAA = phenylacetic acid, SA = salicylic acid, BzA = benzoic acid, ABA =
abscisic acid, ACC = 1-aminocyclopropane-1-carboxylic acid
PB  - Daejeon: International Association For Plant Biotechnology
C3  - Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea
T1  - Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling
SP  - 484
EP  - 484
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6112
ER  - 

@conference{
author = "Ninković, Slavica and Banjac, Nevena and Stanišić, Mariana and Smailagić, Dijana and Ćosić, Tatjana and Živanović, Branka and Dobrev, Petre I. and Motyka, Vaclav",
year = "2023",
abstract = "Root-exuded compounds play a key role in the regulation of plant allelopathic interactions. In contrast to
numerous studies showing that many secondary metabolites have an allelopathic function, evidence of
exudation of phytohormones into the rhizosphere and their involvement in allelopathic interactions is scarce.
We postulated that phytohormones secreted by roots into the environment may act as allelochemicals that
affect the growth performance of neighboring plants. Hairy root culture media of two distinct species, the fruit
tree species Malus domestica cv. Golden delicious[1] and the herbaceous weed species Chenopodium murale
[2,3] were collected and analyzed by ultra-high-performance liquid chromatography coupled with tandem mass
spectrometry (UHPLC-MS/MS). We found that most of plant growth substances exuded by the hairy roots of
C. murale and M. domestica were assorted from fraction A (96.8% and even 98.9%, respectively) including
OxIAA, PAA, SA, BzA, and ABA, with SA and BzA being the most abundant, whereas those from fraction B,
including cytokinins and the ethylene precursor ACC, accounted for only 1% of the regulatory substances
detected in both species. Exogenous application of SA at concentrations released by the hairy roots of the
respective plant species and accumulated in the culture media for four weeks significantly affected the hairy
root growth of the other species and also the shoot and root growth of the Arabidopsis seedlings. The
disruptive effect of SA in the exuded concentration on the membrane potential of Arabidopsis root cells was
determined. The data obtained would be useful for designing further studies aimed at elucidating the
contribution and role of exuded phytohormones to overall allelopathic potential of these two plant species.
OxIAA = 2-oxindole-3-acetic acid, PAA = phenylacetic acid, SA = salicylic acid, BzA = benzoic acid, ABA =
abscisic acid, ACC = 1-aminocyclopropane-1-carboxylic acid",
publisher = "Daejeon: International Association For Plant Biotechnology",
journal = "Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea",
title = "Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling",
pages = "484-484",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6112"
}

Ninković, S., Banjac, N., Stanišić, M., Smailagić, D., Ćosić, T., Živanović, B., Dobrev, P. I.,& Motyka, V.. (2023). Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling. in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea
Daejeon: International Association For Plant Biotechnology., 484-484.
https://hdl.handle.net/21.15107/rcub_ibiss_6112

Ninković S, Banjac N, Stanišić M, Smailagić D, Ćosić T, Živanović B, Dobrev PI, Motyka V. Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling. in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea. 2023;:484-484.
https://hdl.handle.net/21.15107/rcub_ibiss_6112 .

Ninković, Slavica, Banjac, Nevena, Stanišić, Mariana, Smailagić, Dijana, Ćosić, Tatjana, Živanović, Branka, Dobrev, Petre I., Motyka, Vaclav, "Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling" in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea (2023):484-484,
https://hdl.handle.net/21.15107/rcub_ibiss_6112 .

TY  - JOUR
AU  - Ćosić, Tatjana
AU  - Raspor, Martin
AU  - Motyka, Vaclav
AU  - Cingel, Aleksandar
AU  - Ninković, Slavica
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5856
AB  - Kohlrabi (Brassica oleracea var. gongylodes) is a vegetable cultivated for its edible stem tuber. Although valued for its nutritional properties and tolerance to abiotic stress, kohlrabi is one of the least studied brassicas. In this review, we summarize the results of our decade-long research on in vitro morphogenesis of kohlrabi, starting from 2013. Protocols for efficient in vitro regeneration with minimal requirements for external application of plant growth regulators (PGRs) have been developed, both by somatic embryogenesis (SE) and by de novo shoot organogenesis (DNSO). Efficient regeneration by direct SE was achieved using immature zygotic embryos as explants incubated on PGR-free media, and the production process was maintained in culture thanks to highly efficient secondary SE. Conversely, efficient regeneration by indirect DNSO was achieved using entire seedlings as explants and adding only cytokinin (CK) without the need for exogenous auxin. Comprehensive phytohormone analyses revealed that different exogenously applied CKs differentially affected the composition of endogenous phytohormones and induced changes in the expression of cell cycle-related genes and other genes involved in the organogenic response. The addition of high sucrose concentrations to the nutrient media failed to induce the formation of stem tubers in in vitro culture, but revealed a complex interaction with exogenously applied CKs, interfering with both the endogenous phytohormonome and the expression of organogenesis-related genes. Our work has provided substantial biotechnological advances in the field of in vitro regeneration of kohlrabi, as well as in understanding the underlying phytohormonal regulation. The review aims to introduce kohlrabi to the scientific community as a model system for both basic and applied research, while we continue seeking answers to the outstanding questions and trying to pave the way for the development of more resistant varieties.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - In Vitro Growth and Regeneration of Brassica oleracea var. gongylodes: A Decade of Research
IS  - 6
VL  - 9
DO  - 10.3390/horticulturae9060674
SP  - 674
ER  - 

@article{
author = "Ćosić, Tatjana and Raspor, Martin and Motyka, Vaclav and Cingel, Aleksandar and Ninković, Slavica",
year = "2023",
abstract = "Kohlrabi (Brassica oleracea var. gongylodes) is a vegetable cultivated for its edible stem tuber. Although valued for its nutritional properties and tolerance to abiotic stress, kohlrabi is one of the least studied brassicas. In this review, we summarize the results of our decade-long research on in vitro morphogenesis of kohlrabi, starting from 2013. Protocols for efficient in vitro regeneration with minimal requirements for external application of plant growth regulators (PGRs) have been developed, both by somatic embryogenesis (SE) and by de novo shoot organogenesis (DNSO). Efficient regeneration by direct SE was achieved using immature zygotic embryos as explants incubated on PGR-free media, and the production process was maintained in culture thanks to highly efficient secondary SE. Conversely, efficient regeneration by indirect DNSO was achieved using entire seedlings as explants and adding only cytokinin (CK) without the need for exogenous auxin. Comprehensive phytohormone analyses revealed that different exogenously applied CKs differentially affected the composition of endogenous phytohormones and induced changes in the expression of cell cycle-related genes and other genes involved in the organogenic response. The addition of high sucrose concentrations to the nutrient media failed to induce the formation of stem tubers in in vitro culture, but revealed a complex interaction with exogenously applied CKs, interfering with both the endogenous phytohormonome and the expression of organogenesis-related genes. Our work has provided substantial biotechnological advances in the field of in vitro regeneration of kohlrabi, as well as in understanding the underlying phytohormonal regulation. The review aims to introduce kohlrabi to the scientific community as a model system for both basic and applied research, while we continue seeking answers to the outstanding questions and trying to pave the way for the development of more resistant varieties.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "In Vitro Growth and Regeneration of Brassica oleracea var. gongylodes: A Decade of Research",
number = "6",
volume = "9",
doi = "10.3390/horticulturae9060674",
pages = "674"
}

Ćosić, T., Raspor, M., Motyka, V., Cingel, A.,& Ninković, S.. (2023). In Vitro Growth and Regeneration of Brassica oleracea var. gongylodes: A Decade of Research. in Horticulturae
Basel: MDPI., 9(6), 674.
https://doi.org/10.3390/horticulturae9060674

Ćosić T, Raspor M, Motyka V, Cingel A, Ninković S. In Vitro Growth and Regeneration of Brassica oleracea var. gongylodes: A Decade of Research. in Horticulturae. 2023;9(6):674.
doi:10.3390/horticulturae9060674 .

Ćosić, Tatjana, Raspor, Martin, Motyka, Vaclav, Cingel, Aleksandar, Ninković, Slavica, "In Vitro Growth and Regeneration of Brassica oleracea var. gongylodes: A Decade of Research" in Horticulturae, 9, no. 6 (2023):674,
https://doi.org/10.3390/horticulturae9060674 . .

TY  - CONF
AU  - Smailagić, Dijana
AU  - Dragišić Maksimović, Jelena
AU  - Stupar, Sofija
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Stanišić, Mariana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5067
AB  - Apple species are exclusively rich source of dihydrochalcones phloretin and phloridzin, phenolic compounds with elusive role in planta but suggested auto-allelochemical features related to 
‘apple replant disease’ (ARD). Our recent study emphasized phytotoxic action of phloretin towards 
other plant species such as model plant Arabidopsis as a consequence of disturbed auxin homeostasis in roots and altered chloroplast structure and function. Since elevated production and 
accumulation of reactive oxygen species (ROS) in plant cells is the one of the most common effect 
of allelochemicals, this study aimed to analyze the antioxidant status of Arabidopsis seedlings 
treated with phloretin. Total phenolic content (TPC) and total antioxidant capacity (TAC), as well 
as antioxidant enzymes activities were determined spectrophotometrically in treated and control 
seedlings at 5th and 10th day after germination (DAG). TAC increased c.a. 280% and 200% relative to 
control at 5th and 10th DAG respectively, presumably due to highly elevated TPC in treated seedlings (c.a. 150%). At the same time, the activity of antioxidant enzymes significantly decreased. The 
activity of catalase (CAT) was reduced by c.a. 40% and guaiacol peroxidase (POD) activities by 93% 
and 47% at 5th and 10th DAG, respectively. The activity of superoxide dismutase (SOD) was reduced 
by 55% and 18% at 5th and 10th DAG, respectively. The burst of antioxidant capacity of phloretin 
treated Arabidopsis seedlings resulted in significantly decreased antioxidant enzymes activities 
presumably due to strong antioxidant properties of absorbed phloretin. However, phloretin inhibition of antioxidant enzymatic activity due to its direct interaction with enzymes is object of 
further investigation.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Phloretin, dihydrochalcone from apple tree, bursts antioxidant capacity of tested Arabidopsis seedlings
SP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5067
ER  - 

@conference{
author = "Smailagić, Dijana and Dragišić Maksimović, Jelena and Stupar, Sofija and Banjac, Nevena and Ninković, Slavica and Stanišić, Mariana",
year = "2022",
abstract = "Apple species are exclusively rich source of dihydrochalcones phloretin and phloridzin, phenolic compounds with elusive role in planta but suggested auto-allelochemical features related to 
‘apple replant disease’ (ARD). Our recent study emphasized phytotoxic action of phloretin towards 
other plant species such as model plant Arabidopsis as a consequence of disturbed auxin homeostasis in roots and altered chloroplast structure and function. Since elevated production and 
accumulation of reactive oxygen species (ROS) in plant cells is the one of the most common effect 
of allelochemicals, this study aimed to analyze the antioxidant status of Arabidopsis seedlings 
treated with phloretin. Total phenolic content (TPC) and total antioxidant capacity (TAC), as well 
as antioxidant enzymes activities were determined spectrophotometrically in treated and control 
seedlings at 5th and 10th day after germination (DAG). TAC increased c.a. 280% and 200% relative to 
control at 5th and 10th DAG respectively, presumably due to highly elevated TPC in treated seedlings (c.a. 150%). At the same time, the activity of antioxidant enzymes significantly decreased. The 
activity of catalase (CAT) was reduced by c.a. 40% and guaiacol peroxidase (POD) activities by 93% 
and 47% at 5th and 10th DAG, respectively. The activity of superoxide dismutase (SOD) was reduced 
by 55% and 18% at 5th and 10th DAG, respectively. The burst of antioxidant capacity of phloretin 
treated Arabidopsis seedlings resulted in significantly decreased antioxidant enzymes activities 
presumably due to strong antioxidant properties of absorbed phloretin. However, phloretin inhibition of antioxidant enzymatic activity due to its direct interaction with enzymes is object of 
further investigation.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Phloretin, dihydrochalcone from apple tree, bursts antioxidant capacity of tested Arabidopsis seedlings",
pages = "73",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5067"
}

Smailagić, D., Dragišić Maksimović, J., Stupar, S., Banjac, N., Ninković, S.,& Stanišić, M.. (2022). Phloretin, dihydrochalcone from apple tree, bursts antioxidant capacity of tested Arabidopsis seedlings. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 73.
https://hdl.handle.net/21.15107/rcub_ibiss_5067

Smailagić D, Dragišić Maksimović J, Stupar S, Banjac N, Ninković S, Stanišić M. Phloretin, dihydrochalcone from apple tree, bursts antioxidant capacity of tested Arabidopsis seedlings. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:73.
https://hdl.handle.net/21.15107/rcub_ibiss_5067 .

Smailagić, Dijana, Dragišić Maksimović, Jelena, Stupar, Sofija, Banjac, Nevena, Ninković, Slavica, Stanišić, Mariana, "Phloretin, dihydrochalcone from apple tree, bursts antioxidant capacity of tested Arabidopsis seedlings" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):73,
https://hdl.handle.net/21.15107/rcub_ibiss_5067 .

TY  - CONF
AU  - Bondokić, Jasna
AU  - Trajković, Milena
AU  - Dragišić Maksimović, Jelena
AU  - Smailagić, Dijana
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Stanišić, Mariana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5064
AB  - Phloretin is phenolic compound specifically produced in apple tree species and secreted into the soil as a response to biotic and abiotic factors. Since our previous results indicated roots as the primary site of phloretin phytotoxic action, this study focused on assessment of phloretin effects on the root growth of weed (Chenopodium murale and Lotus corniculatus),  crop (Solanum tuberosum) and woody species (Castanea sativa and Malus × domestica Borkh.). Significant decrease in root biomass production in liquid growth medium enriched with 500 µM phloretin was observed both after 2 and 4 weeks of treatment in all tested species. The percentages of roots growth inhibition were as follows: Chenopodium murale (95% and 89,1%), Lotus corniculatus (69,4% and 81,8%), Solanum tuberosum (88,4% and 84,7%), Castanea sativa (85,6% and 100%) and Malus × domestica Borkh. (65,3% and 87,4%, respectively). Total antioxidant capacity (TAC), total phenolic content (TPC), as well as antioxidant enzymes activities were determined spectrophotometrically in treated and control Chenopodium murale roots after 4 weeks of phloretin treatment. TAC increased 44,1% presumably due to elevated TPC (25,8%) in treated roots. However, there were no significant differences in catalase (CAT), guaiacol peroxidase (POD) and superoxide dismutase (SOD) activities in control and treated roots after 4 weeks of treatment. Increased antioxidant activity in treated roots is presumably the consequence of known phloretin antioxidant potential.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - The effect of phloretin on root growth and antioxidant activity in selected plant species in vitro
SP  - 81
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5064
ER  - 

@conference{
author = "Bondokić, Jasna and Trajković, Milena and Dragišić Maksimović, Jelena and Smailagić, Dijana and Banjac, Nevena and Ninković, Slavica and Stanišić, Mariana",
year = "2022",
abstract = "Phloretin is phenolic compound specifically produced in apple tree species and secreted into the soil as a response to biotic and abiotic factors. Since our previous results indicated roots as the primary site of phloretin phytotoxic action, this study focused on assessment of phloretin effects on the root growth of weed (Chenopodium murale and Lotus corniculatus),  crop (Solanum tuberosum) and woody species (Castanea sativa and Malus × domestica Borkh.). Significant decrease in root biomass production in liquid growth medium enriched with 500 µM phloretin was observed both after 2 and 4 weeks of treatment in all tested species. The percentages of roots growth inhibition were as follows: Chenopodium murale (95% and 89,1%), Lotus corniculatus (69,4% and 81,8%), Solanum tuberosum (88,4% and 84,7%), Castanea sativa (85,6% and 100%) and Malus × domestica Borkh. (65,3% and 87,4%, respectively). Total antioxidant capacity (TAC), total phenolic content (TPC), as well as antioxidant enzymes activities were determined spectrophotometrically in treated and control Chenopodium murale roots after 4 weeks of phloretin treatment. TAC increased 44,1% presumably due to elevated TPC (25,8%) in treated roots. However, there were no significant differences in catalase (CAT), guaiacol peroxidase (POD) and superoxide dismutase (SOD) activities in control and treated roots after 4 weeks of treatment. Increased antioxidant activity in treated roots is presumably the consequence of known phloretin antioxidant potential.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "The effect of phloretin on root growth and antioxidant activity in selected plant species in vitro",
pages = "81",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5064"
}

Bondokić, J., Trajković, M., Dragišić Maksimović, J., Smailagić, D., Banjac, N., Ninković, S.,& Stanišić, M.. (2022). The effect of phloretin on root growth and antioxidant activity in selected plant species in vitro. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 81.
https://hdl.handle.net/21.15107/rcub_ibiss_5064

Bondokić J, Trajković M, Dragišić Maksimović J, Smailagić D, Banjac N, Ninković S, Stanišić M. The effect of phloretin on root growth and antioxidant activity in selected plant species in vitro. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:81.
https://hdl.handle.net/21.15107/rcub_ibiss_5064 .

Bondokić, Jasna, Trajković, Milena, Dragišić Maksimović, Jelena, Smailagić, Dijana, Banjac, Nevena, Ninković, Slavica, Stanišić, Mariana, "The effect of phloretin on root growth and antioxidant activity in selected plant species in vitro" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):81,
https://hdl.handle.net/21.15107/rcub_ibiss_5064 .

TY  - CONF
AU  - Smailagić, Dijana
AU  - Marin, Marija
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Stanišić, Mariana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5066
AB  - Phloretin is a phenolic compound unique for apple (Malus × domestica Borkh.) species. Our 
research group recently reported phytotoxic potential of phloretin towards other plant species 
such as model plant Arabidopsis. Since sugars in in vitro growth media could modulate plant 
metabolism, we analyzed phloretin effects on Arabidopsis seedlings grown on the medium with out sucrose and myoinositol (WSM) but enriched with different concentrations of phloretin (0 
to 1500 µM). Phloretin treatment on WSM induced seedlings growth retardation at much lower 
concentrations compared to sucrose and myoinositol enriched medium. Inhibition of primary 
root and leaf growth, and lateral root emergence increased with both phloretin concentration 
and treatment duration (maximal 85.2%, 30.4% and 46.5% at 1500 µM phloretin at 15 days after 
germination - DAG, respectively). Prolonged cultivation (4 weeks) at phloretin concentrations over 
500 µM induced complete growth stagnation and necrosis of the leaves. Transmission electron 
microscopy reviled relocation of chloroplasts within mesophyll cells, shape change into spherical 
form, as well as ultrastructural changes in thylakoid organization in leaves of 10 DAG-old seedlings 
treated with 250 or 500 µM phloretin. Significantly decreased number of starch grains in chloro plasts and chlorophyll a and b content in leaves reflected impaired function of chloroplasts due 
to ultrastructural changes. Phloretin also induced cell wall undulation and folds that resulted in 
irregularly shaped mesophyll cells. The presented study revealed that beside roots that had been 
previously emphasized as primary targets of phloretin action, leaves were also significantly affect ed by phloretin treatment, especially in the aspect of photosynthetic apparatus
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Morpho-physiological and ultrastructural changes of Arabidopsis seedlings treated with phloretin
SP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5066
ER  - 

@conference{
author = "Smailagić, Dijana and Marin, Marija and Banjac, Nevena and Ninković, Slavica and Stanišić, Mariana",
year = "2022",
abstract = "Phloretin is a phenolic compound unique for apple (Malus × domestica Borkh.) species. Our 
research group recently reported phytotoxic potential of phloretin towards other plant species 
such as model plant Arabidopsis. Since sugars in in vitro growth media could modulate plant 
metabolism, we analyzed phloretin effects on Arabidopsis seedlings grown on the medium with out sucrose and myoinositol (WSM) but enriched with different concentrations of phloretin (0 
to 1500 µM). Phloretin treatment on WSM induced seedlings growth retardation at much lower 
concentrations compared to sucrose and myoinositol enriched medium. Inhibition of primary 
root and leaf growth, and lateral root emergence increased with both phloretin concentration 
and treatment duration (maximal 85.2%, 30.4% and 46.5% at 1500 µM phloretin at 15 days after 
germination - DAG, respectively). Prolonged cultivation (4 weeks) at phloretin concentrations over 
500 µM induced complete growth stagnation and necrosis of the leaves. Transmission electron 
microscopy reviled relocation of chloroplasts within mesophyll cells, shape change into spherical 
form, as well as ultrastructural changes in thylakoid organization in leaves of 10 DAG-old seedlings 
treated with 250 or 500 µM phloretin. Significantly decreased number of starch grains in chloro plasts and chlorophyll a and b content in leaves reflected impaired function of chloroplasts due 
to ultrastructural changes. Phloretin also induced cell wall undulation and folds that resulted in 
irregularly shaped mesophyll cells. The presented study revealed that beside roots that had been 
previously emphasized as primary targets of phloretin action, leaves were also significantly affect ed by phloretin treatment, especially in the aspect of photosynthetic apparatus",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Morpho-physiological and ultrastructural changes of Arabidopsis seedlings treated with phloretin",
pages = "72",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5066"
}

Smailagić, D., Marin, M., Banjac, N., Ninković, S.,& Stanišić, M.. (2022). Morpho-physiological and ultrastructural changes of Arabidopsis seedlings treated with phloretin. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 72.
https://hdl.handle.net/21.15107/rcub_ibiss_5066

Smailagić D, Marin M, Banjac N, Ninković S, Stanišić M. Morpho-physiological and ultrastructural changes of Arabidopsis seedlings treated with phloretin. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:72.
https://hdl.handle.net/21.15107/rcub_ibiss_5066 .

Smailagić, Dijana, Marin, Marija, Banjac, Nevena, Ninković, Slavica, Stanišić, Mariana, "Morpho-physiological and ultrastructural changes of Arabidopsis seedlings treated with phloretin" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):72,
https://hdl.handle.net/21.15107/rcub_ibiss_5066 .

TY  - CONF
AU  - Stanišić, Mariana
AU  - Smailagić, Dijana
AU  - Živanović, Branka
AU  - Maksimović, Vuk
AU  - Ninković, Slavica
AU  - Banjac, Nevena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5063
AB  - Phloretin, phenolic compound found exclusively in Apple trees, possesses bioherbicidal potential towards other plant species. Our recent studies emphasized phloretin phytotoxic action towards model plant Arabidopsis [Arabidopsis thaliana (L.) Heynh.] as a consequence of disturbed auxin homeostasis in roots and disrupted normal leaf cell function due to chloroplast ultrastructural changes and decreased chlorophyll a and b levels. Given the known phloretin reactivity that results from its flexible dihydrochalcones structure, this study aimed to elucidate phloretin direct effects on the root plasma membrane, as well as phloretin post-treatment metabolism in Arabidopsis plants. Electrophysiological measurements were performed to assess real-time response of root plasma membrane to exogenously applied phloretin. Exposing Arabidopsis roots to a 250 µM phloretin resulted in rapid and significant depolarization of the root membrane potential with amplitude of about 47mV that confirmed high sensitivity of Arabidopsis to phloretin treatment. Reverse-phase liquid chromatography with mass spectrometry (LC-MS) was used to evaluate post-treatment metabolism of phloretin in Arabidopsis seedlings grown on 500 µM phloretin enriched medium for 10 days. Analyses revealed that phloretin underwent intensive glycosylation within plant tissues. Dominant peaks from treated plants showed a palette of phloretin derivatives mainly mono- and di-glycosides. Their quantity was significantly higher than in control plants, suggesting high uptake and metabolic interconversion of phloretin as an attempt of phloretin detoxification and/or preparation for vacuolar sequestration.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - What happens with phloretin in plants? – Phloretin real-time effects and post-treatment metabolism in treated Arabidopsis seedlings
SP  - 44
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5063
ER  - 

@conference{
author = "Stanišić, Mariana and Smailagić, Dijana and Živanović, Branka and Maksimović, Vuk and Ninković, Slavica and Banjac, Nevena",
year = "2022",
abstract = "Phloretin, phenolic compound found exclusively in Apple trees, possesses bioherbicidal potential towards other plant species. Our recent studies emphasized phloretin phytotoxic action towards model plant Arabidopsis [Arabidopsis thaliana (L.) Heynh.] as a consequence of disturbed auxin homeostasis in roots and disrupted normal leaf cell function due to chloroplast ultrastructural changes and decreased chlorophyll a and b levels. Given the known phloretin reactivity that results from its flexible dihydrochalcones structure, this study aimed to elucidate phloretin direct effects on the root plasma membrane, as well as phloretin post-treatment metabolism in Arabidopsis plants. Electrophysiological measurements were performed to assess real-time response of root plasma membrane to exogenously applied phloretin. Exposing Arabidopsis roots to a 250 µM phloretin resulted in rapid and significant depolarization of the root membrane potential with amplitude of about 47mV that confirmed high sensitivity of Arabidopsis to phloretin treatment. Reverse-phase liquid chromatography with mass spectrometry (LC-MS) was used to evaluate post-treatment metabolism of phloretin in Arabidopsis seedlings grown on 500 µM phloretin enriched medium for 10 days. Analyses revealed that phloretin underwent intensive glycosylation within plant tissues. Dominant peaks from treated plants showed a palette of phloretin derivatives mainly mono- and di-glycosides. Their quantity was significantly higher than in control plants, suggesting high uptake and metabolic interconversion of phloretin as an attempt of phloretin detoxification and/or preparation for vacuolar sequestration.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "What happens with phloretin in plants? – Phloretin real-time effects and post-treatment metabolism in treated Arabidopsis seedlings",
pages = "44",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5063"
}

Stanišić, M., Smailagić, D., Živanović, B., Maksimović, V., Ninković, S.,& Banjac, N.. (2022). What happens with phloretin in plants? – Phloretin real-time effects and post-treatment metabolism in treated Arabidopsis seedlings. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 44.
https://hdl.handle.net/21.15107/rcub_ibiss_5063

Stanišić M, Smailagić D, Živanović B, Maksimović V, Ninković S, Banjac N. What happens with phloretin in plants? – Phloretin real-time effects and post-treatment metabolism in treated Arabidopsis seedlings. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:44.
https://hdl.handle.net/21.15107/rcub_ibiss_5063 .

Stanišić, Mariana, Smailagić, Dijana, Živanović, Branka, Maksimović, Vuk, Ninković, Slavica, Banjac, Nevena, "What happens with phloretin in plants? – Phloretin real-time effects and post-treatment metabolism in treated Arabidopsis seedlings" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):44,
https://hdl.handle.net/21.15107/rcub_ibiss_5063 .

TY  - CONF
AU  - Ćosić, Tatjana
AU  - Motyka, Václav
AU  - Raspor, Martin
AU  - Sajid, Sumbal
AU  - Savić, Jelena
AU  - Devrnja, Nina
AU  - Dobrev, Petre I.
AU  - Ninković, Slavica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5364
AB  - This study fathoms a comprehensive hormone profiling of four growth stages of kohlrabi
(Brassica oleracea var. gongylodes) seedlings in vitro (T1-T4), including the effects of cytokinins, CKs
(trans-zeatin, transZ; thidiazuron, TDZ), and high sucrose concentration (6% and 9%). Depending
on the type of investigated phytohormone and its metabolite, individual changes were observed
on a time scale, showing specific hormone profiles. A significant increase in the content of endogenous
zeatin nucleobases and gibberellin GA20 was detected at T2 stage (plantlets with two
emerged leaves). However, levels of jasmonic acid, JA-isoleucine, indole-3-acetic acid (IAA) and
indole-3-acetamide significantly increased as development progressed (T3), whereas content of
most of analysed IAA metabolites decreased. Supplementing growth media with CKs induced de
novo shoot formation during seedling development, and both CK and sucrose treatments caused
significant changes in levels of most of the phytohormone groups at each developmental stage
compared to control. Principal component analysis indicated that sucrose treatments, particularly
9%, had a stronger effect on the content of endogenous hormones than the CK treatments. The
difference between two CK treatments proved to be remarkable at T1, T2, and T3 stages, while
at the last stage difference was much smaller. The joint application of CKs and high sucrose concentration
showed diverse relations depending on the metabolite, growth stage and amount of
sucrose used. The most striking increase was induced in T4 for zeatin nucleobases when transZ
and 9% sucrose were applied. Our results indicate specific patterns of metabolic balance during
kohlrabi in vitro development and regeneration, revealing a complex interplay of different phytohormonal
groups with CKs and sucrose.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Phytohormone homeostasis in in vitro grown kohlrabi: the effect of cytokinins and sucrose
SP  - 24
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5364
ER  - 

@conference{
author = "Ćosić, Tatjana and Motyka, Václav and Raspor, Martin and Sajid, Sumbal and Savić, Jelena and Devrnja, Nina and Dobrev, Petre I. and Ninković, Slavica",
year = "2022",
abstract = "This study fathoms a comprehensive hormone profiling of four growth stages of kohlrabi
(Brassica oleracea var. gongylodes) seedlings in vitro (T1-T4), including the effects of cytokinins, CKs
(trans-zeatin, transZ; thidiazuron, TDZ), and high sucrose concentration (6% and 9%). Depending
on the type of investigated phytohormone and its metabolite, individual changes were observed
on a time scale, showing specific hormone profiles. A significant increase in the content of endogenous
zeatin nucleobases and gibberellin GA20 was detected at T2 stage (plantlets with two
emerged leaves). However, levels of jasmonic acid, JA-isoleucine, indole-3-acetic acid (IAA) and
indole-3-acetamide significantly increased as development progressed (T3), whereas content of
most of analysed IAA metabolites decreased. Supplementing growth media with CKs induced de
novo shoot formation during seedling development, and both CK and sucrose treatments caused
significant changes in levels of most of the phytohormone groups at each developmental stage
compared to control. Principal component analysis indicated that sucrose treatments, particularly
9%, had a stronger effect on the content of endogenous hormones than the CK treatments. The
difference between two CK treatments proved to be remarkable at T1, T2, and T3 stages, while
at the last stage difference was much smaller. The joint application of CKs and high sucrose concentration
showed diverse relations depending on the metabolite, growth stage and amount of
sucrose used. The most striking increase was induced in T4 for zeatin nucleobases when transZ
and 9% sucrose were applied. Our results indicate specific patterns of metabolic balance during
kohlrabi in vitro development and regeneration, revealing a complex interplay of different phytohormonal
groups with CKs and sucrose.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Phytohormone homeostasis in in vitro grown kohlrabi: the effect of cytokinins and sucrose",
pages = "24",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5364"
}

Ćosić, T., Motyka, V., Raspor, M., Sajid, S., Savić, J., Devrnja, N., Dobrev, P. I.,& Ninković, S.. (2022). Phytohormone homeostasis in in vitro grown kohlrabi: the effect of cytokinins and sucrose. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 24.
https://hdl.handle.net/21.15107/rcub_ibiss_5364

Ćosić T, Motyka V, Raspor M, Sajid S, Savić J, Devrnja N, Dobrev PI, Ninković S. Phytohormone homeostasis in in vitro grown kohlrabi: the effect of cytokinins and sucrose. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:24.
https://hdl.handle.net/21.15107/rcub_ibiss_5364 .

Ćosić, Tatjana, Motyka, Václav, Raspor, Martin, Sajid, Sumbal, Savić, Jelena, Devrnja, Nina, Dobrev, Petre I., Ninković, Slavica, "Phytohormone homeostasis in in vitro grown kohlrabi: the effect of cytokinins and sucrose" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):24,
https://hdl.handle.net/21.15107/rcub_ibiss_5364 .

TY  - CHAP
AU  - Stanišić, Mariana
AU  - Ninković, Slavica
AU  - Banjac, Nevena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6567
AB  - Phloridzin and phloretin are dihydrochalcones highly specific to domestic apple (Malus × domestica
Borkh.) and other species from genus Malus. Auto-allelopathic potential of these phenolics was recognized in‘Apple Replant Disease’(ARD) characterized by stunted growth, reduced root system and low yields
in apple trees replanted on lands that previously supported apple orchards. Physiological basis of phloridzin and phloretin autotoxicity are ultrastructural and molecular alterations that include increased catalase, peroxidases and superoxide dismutase activities, raised malondialdehyde and proline contents, and
high expression of genes involved in cell defence system. The latest research on model plant Arabidopsis
thaliana indicates phytotoxicity of these dihydrochalcones towards other plant species. Phloretin inhibits
A. thaliana seedlings growth and development inducing agravitropic phenotype and morphological and
ultrastructural malformations in treated seedlings. Altered expression of CDKA1;1, CDKB2;1, CYCA3;1 and
CYCB2;4 cell cycle genes and genes involved in auxin homeostasis (PINs, AUX1, LAX3, ABCBs, TAA1 and YUCs)
and gibberellin biosynthesis (GA20ox2 and GA3ox1) are in the physiological bases of phloretin phytotoxicity. This mechanism makes phloretin a prospective candidate for an eco-friendly bioherbicide and paves
the way for further research of phloretin role in ARD
AB  - Sekundarni metaboliti jabuke (Malus × domestica Borkh.) florizin i floretin su dihidrohalkoni visoko
specifični za vrste roda Malus. Autoalelopatski potencijal ovih fenolnih jedinjenja se ogleda u suzbijanju
rasta klijanca i sadnica zasađenih na mestu prvobitnih voćnjaka jabuke. U osnovi njihove fitotoksičnosti su
ultrastrukturne i molekularne promene koje se ogledaju u povećanju sadržaja malondialdehida, prolina i
aktivnosti antioksidativnih enzima: superoksid-dismutaze, peroksidaza i katalaza, kao i nivoa ekspresije
proteina uključenih u odbrambene mehanizme ćelije. Najnovija istraživanja ukazuju na fitotoksičnost ovih
dihidrohalkona i prema drugim biljnim vrstama, kao npr. korovskoj model biljci Arabidopsis thaliana. Tretman floretinom izaziva značajnu inhibiciju rastenja i razvića klijanaca A. thaliana, poremećaj gravitropskog
odgovora i pojavu morfoloških i ultrastrukturnih malformacija u čijoj osnovi leži promena ekspresije
CDKA1;1, CDKB2;1, CYCA3;1 i CYCB2;4 gena ćelijskog ciklusa, kao i gena uključenih u održanje homeostaze
auksina (PINs, AUX1, LAX3, ABCBs, TAA1 i YUCs) i biosinteze giberelina (GA20ox2 i GA3ox1). Ovo otvara
mogućnost za dalja istraživanja njihovog alelopatskog delovanja ali i potencijalnu primenu kao ekološki
bezbednih bioherbicida
PB  - Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade
T2  - Trends in Molecular Biology
T1  - Dihidrohalkoni jabuke florizin i floretin kao nove alelopatske supstance
T1  - Apple dihydrochalcones phloridzin and phloretin as novel allelochemicals
IS  - 2
SP  - 223
EP  - 232
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6567
ER  - 

@inbook{
author = "Stanišić, Mariana and Ninković, Slavica and Banjac, Nevena",
year = "2022",
abstract = "Phloridzin and phloretin are dihydrochalcones highly specific to domestic apple (Malus × domestica
Borkh.) and other species from genus Malus. Auto-allelopathic potential of these phenolics was recognized in‘Apple Replant Disease’(ARD) characterized by stunted growth, reduced root system and low yields
in apple trees replanted on lands that previously supported apple orchards. Physiological basis of phloridzin and phloretin autotoxicity are ultrastructural and molecular alterations that include increased catalase, peroxidases and superoxide dismutase activities, raised malondialdehyde and proline contents, and
high expression of genes involved in cell defence system. The latest research on model plant Arabidopsis
thaliana indicates phytotoxicity of these dihydrochalcones towards other plant species. Phloretin inhibits
A. thaliana seedlings growth and development inducing agravitropic phenotype and morphological and
ultrastructural malformations in treated seedlings. Altered expression of CDKA1;1, CDKB2;1, CYCA3;1 and
CYCB2;4 cell cycle genes and genes involved in auxin homeostasis (PINs, AUX1, LAX3, ABCBs, TAA1 and YUCs)
and gibberellin biosynthesis (GA20ox2 and GA3ox1) are in the physiological bases of phloretin phytotoxicity. This mechanism makes phloretin a prospective candidate for an eco-friendly bioherbicide and paves
the way for further research of phloretin role in ARD, Sekundarni metaboliti jabuke (Malus × domestica Borkh.) florizin i floretin su dihidrohalkoni visoko
specifični za vrste roda Malus. Autoalelopatski potencijal ovih fenolnih jedinjenja se ogleda u suzbijanju
rasta klijanca i sadnica zasađenih na mestu prvobitnih voćnjaka jabuke. U osnovi njihove fitotoksičnosti su
ultrastrukturne i molekularne promene koje se ogledaju u povećanju sadržaja malondialdehida, prolina i
aktivnosti antioksidativnih enzima: superoksid-dismutaze, peroksidaza i katalaza, kao i nivoa ekspresije
proteina uključenih u odbrambene mehanizme ćelije. Najnovija istraživanja ukazuju na fitotoksičnost ovih
dihidrohalkona i prema drugim biljnim vrstama, kao npr. korovskoj model biljci Arabidopsis thaliana. Tretman floretinom izaziva značajnu inhibiciju rastenja i razvića klijanaca A. thaliana, poremećaj gravitropskog
odgovora i pojavu morfoloških i ultrastrukturnih malformacija u čijoj osnovi leži promena ekspresije
CDKA1;1, CDKB2;1, CYCA3;1 i CYCB2;4 gena ćelijskog ciklusa, kao i gena uključenih u održanje homeostaze
auksina (PINs, AUX1, LAX3, ABCBs, TAA1 i YUCs) i biosinteze giberelina (GA20ox2 i GA3ox1). Ovo otvara
mogućnost za dalja istraživanja njihovog alelopatskog delovanja ali i potencijalnu primenu kao ekološki
bezbednih bioherbicida",
publisher = "Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade",
journal = "Trends in Molecular Biology",
booktitle = "Dihidrohalkoni jabuke florizin i floretin kao nove alelopatske supstance, Apple dihydrochalcones phloridzin and phloretin as novel allelochemicals",
number = "2",
pages = "223-232",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6567"
}

Stanišić, M., Ninković, S.,& Banjac, N.. (2022). Dihidrohalkoni jabuke florizin i floretin kao nove alelopatske supstance. in Trends in Molecular Biology
Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade.(2), 223-232.
https://hdl.handle.net/21.15107/rcub_ibiss_6567

Stanišić M, Ninković S, Banjac N. Dihidrohalkoni jabuke florizin i floretin kao nove alelopatske supstance. in Trends in Molecular Biology. 2022;(2):223-232.
https://hdl.handle.net/21.15107/rcub_ibiss_6567 .

Stanišić, Mariana, Ninković, Slavica, Banjac, Nevena, "Dihidrohalkoni jabuke florizin i floretin kao nove alelopatske supstance" in Trends in Molecular Biology, no. 2 (2022):223-232,
https://hdl.handle.net/21.15107/rcub_ibiss_6567 .

TY  - JOUR
AU  - Ćosić, Tatjana
AU  - Motyka, Vaclav
AU  - Raspor, Martin
AU  - Sajid, Sumbal
AU  - Devrnja, Nina
AU  - Dobrev, Petre I.
AU  - Ninković, Slavica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5117
AB  - The establishment of an efficient protocol for in vitro growth and regeneration of kohlrabi (Brassica oleracea var. gongylodes) allowed us to closely examine the phytohormone profiles of kohlrabi seedlings at four growth stages (T1–T4), additionally including the effects of cytokinins (CKs)—trans-zeatin (transZ) and thidiazuron (TDZ)—and high sucrose concentrations (6% and 9%). Resulting phytohormone profiles showed complex time-course patterns. At the T2 stage of control kohlrabi plantlets (with two emerged true leaves), levels of endogenous CK free bases and gibberellin GA20 increased, while increases in jasmonic acid (JA), JA-isoleucine (JA-Ile), indole-3-acetic acid (IAA) and indole-3-acetamide (IAM) peaked later, at T3. At the same time, the content of most of the analyzed IAA metabolites decreased. Supplementing growth media with CK induced de novo formation of shoots, while both CK and sucrose treatments caused important changes in most of the phytohormone groups at each developmental stage, compared to control. Principal component analysis (PCA) showed that sucrose treatment, especially at 9%, had a stronger effect on the content of endogenous hormones than CK treatments. Correlation analysis showed that the dynamic balance between the levels of certain bioactive phytohormone forms and some of their metabolites could be lost or reversed at particular growth stages and under certain CK or sucrose treatments, with correlation values changing between strongly positive and strongly negative. Our results indicate that the kohlrabi phytohormonome is a highly dynamic system that changes greatly along the developmental time scale and also during de novo shoot formation, depending on exogenous factors such as the presence of growth regulators and different sucrose concentrations in the growth media, and that it interacts intensively with these factors to facilitate certain responses.
PB  - Basel: MDPI
T2  - Life
T1  - Comprehensive Phytohormone Profiling of Kohlrabi during In Vitro Growth and Regeneration: The Interplay with Cytokinin and Sucrose
IS  - 10
VL  - 12
DO  - 10.3390/life12101585
SP  - 1585
ER  - 

@article{
author = "Ćosić, Tatjana and Motyka, Vaclav and Raspor, Martin and Sajid, Sumbal and Devrnja, Nina and Dobrev, Petre I. and Ninković, Slavica",
year = "2022",
abstract = "The establishment of an efficient protocol for in vitro growth and regeneration of kohlrabi (Brassica oleracea var. gongylodes) allowed us to closely examine the phytohormone profiles of kohlrabi seedlings at four growth stages (T1–T4), additionally including the effects of cytokinins (CKs)—trans-zeatin (transZ) and thidiazuron (TDZ)—and high sucrose concentrations (6% and 9%). Resulting phytohormone profiles showed complex time-course patterns. At the T2 stage of control kohlrabi plantlets (with two emerged true leaves), levels of endogenous CK free bases and gibberellin GA20 increased, while increases in jasmonic acid (JA), JA-isoleucine (JA-Ile), indole-3-acetic acid (IAA) and indole-3-acetamide (IAM) peaked later, at T3. At the same time, the content of most of the analyzed IAA metabolites decreased. Supplementing growth media with CK induced de novo formation of shoots, while both CK and sucrose treatments caused important changes in most of the phytohormone groups at each developmental stage, compared to control. Principal component analysis (PCA) showed that sucrose treatment, especially at 9%, had a stronger effect on the content of endogenous hormones than CK treatments. Correlation analysis showed that the dynamic balance between the levels of certain bioactive phytohormone forms and some of their metabolites could be lost or reversed at particular growth stages and under certain CK or sucrose treatments, with correlation values changing between strongly positive and strongly negative. Our results indicate that the kohlrabi phytohormonome is a highly dynamic system that changes greatly along the developmental time scale and also during de novo shoot formation, depending on exogenous factors such as the presence of growth regulators and different sucrose concentrations in the growth media, and that it interacts intensively with these factors to facilitate certain responses.",
publisher = "Basel: MDPI",
journal = "Life",
title = "Comprehensive Phytohormone Profiling of Kohlrabi during In Vitro Growth and Regeneration: The Interplay with Cytokinin and Sucrose",
number = "10",
volume = "12",
doi = "10.3390/life12101585",
pages = "1585"
}

Ćosić, T., Motyka, V., Raspor, M., Sajid, S., Devrnja, N., Dobrev, P. I.,& Ninković, S.. (2022). Comprehensive Phytohormone Profiling of Kohlrabi during In Vitro Growth and Regeneration: The Interplay with Cytokinin and Sucrose. in Life
Basel: MDPI., 12(10), 1585.
https://doi.org/10.3390/life12101585

Ćosić T, Motyka V, Raspor M, Sajid S, Devrnja N, Dobrev PI, Ninković S. Comprehensive Phytohormone Profiling of Kohlrabi during In Vitro Growth and Regeneration: The Interplay with Cytokinin and Sucrose. in Life. 2022;12(10):1585.
doi:10.3390/life12101585 .

Ćosić, Tatjana, Motyka, Vaclav, Raspor, Martin, Sajid, Sumbal, Devrnja, Nina, Dobrev, Petre I., Ninković, Slavica, "Comprehensive Phytohormone Profiling of Kohlrabi during In Vitro Growth and Regeneration: The Interplay with Cytokinin and Sucrose" in Life, 12, no. 10 (2022):1585,
https://doi.org/10.3390/life12101585 . .

TY  - JOUR
AU  - Smailagić, Dijana
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Savić, Jelena
AU  - Ćosić, Tatjana
AU  - Pěnčík, Aleš
AU  - Ćalić, Dušica
AU  - Bogdanović, Milica
AU  - Trajković, Milena
AU  - Stanišić, Mariana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5065
AB  - Apple species are the unique naturally rich source of dihydrochalcones, phenolic compounds with an elusive role in planta, but suggested auto-allelochemical features related to “apple replant disease” (ARD). Our aim was to elucidate the physiological basis of the phytotoxic action of dihydrochalcone phloretin in the model plant Arabidopsis and to promote phloretin as a new prospective eco-friendly phytotoxic compound. Phloretin treatment induced a significant dose-dependent growth retardation and severe morphological abnormalities and agravitropic behavior in Arabidopsis seedlings. Histological examination revealed a reduced starch content in the columella cells and a serious disturbance in root architecture, which resulted in the reduction in length of meristematic and elongation zones. Significantly disturbed auxin metabolome profile in roots with a particularly increased content of IAA accumulated in the lateral parts of the root apex, accompanied by changes in the expression of auxin biosynthetic and transport genes, especially PIN1, PIN3, PIN7, and ABCB1, indicates the role of auxin in physiological basis of phloretin-induced growth retardation. The results reveal a disturbance of auxin homeostasis as the main mechanism of phytotoxic action of phloretin. This mechanism makes phloretin a prospective candidate for an eco-friendly bioherbicide and paves the way for further research of phloretin role in ARD.
PB  - Lausanne: Frontiers Media SA
T2  - Frontiers in Plant Science
T1  - New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action
VL  - 13
DO  - 0.3389/fpls.2022.875528
SP  - 875528
ER  - 

@article{
author = "Smailagić, Dijana and Banjac, Nevena and Ninković, Slavica and Savić, Jelena and Ćosić, Tatjana and Pěnčík, Aleš and Ćalić, Dušica and Bogdanović, Milica and Trajković, Milena and Stanišić, Mariana",
year = "2022",
abstract = "Apple species are the unique naturally rich source of dihydrochalcones, phenolic compounds with an elusive role in planta, but suggested auto-allelochemical features related to “apple replant disease” (ARD). Our aim was to elucidate the physiological basis of the phytotoxic action of dihydrochalcone phloretin in the model plant Arabidopsis and to promote phloretin as a new prospective eco-friendly phytotoxic compound. Phloretin treatment induced a significant dose-dependent growth retardation and severe morphological abnormalities and agravitropic behavior in Arabidopsis seedlings. Histological examination revealed a reduced starch content in the columella cells and a serious disturbance in root architecture, which resulted in the reduction in length of meristematic and elongation zones. Significantly disturbed auxin metabolome profile in roots with a particularly increased content of IAA accumulated in the lateral parts of the root apex, accompanied by changes in the expression of auxin biosynthetic and transport genes, especially PIN1, PIN3, PIN7, and ABCB1, indicates the role of auxin in physiological basis of phloretin-induced growth retardation. The results reveal a disturbance of auxin homeostasis as the main mechanism of phytotoxic action of phloretin. This mechanism makes phloretin a prospective candidate for an eco-friendly bioherbicide and paves the way for further research of phloretin role in ARD.",
publisher = "Lausanne: Frontiers Media SA",
journal = "Frontiers in Plant Science",
title = "New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action",
volume = "13",
doi = "0.3389/fpls.2022.875528",
pages = "875528"
}

Smailagić, D., Banjac, N., Ninković, S., Savić, J., Ćosić, T., Pěnčík, A., Ćalić, D., Bogdanović, M., Trajković, M.,& Stanišić, M.. (2022). New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action. in Frontiers in Plant Science
Lausanne: Frontiers Media SA., 13, 875528.
https://doi.org/0.3389/fpls.2022.875528

Smailagić D, Banjac N, Ninković S, Savić J, Ćosić T, Pěnčík A, Ćalić D, Bogdanović M, Trajković M, Stanišić M. New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action. in Frontiers in Plant Science. 2022;13:875528.
doi:0.3389/fpls.2022.875528 .

Smailagić, Dijana, Banjac, Nevena, Ninković, Slavica, Savić, Jelena, Ćosić, Tatjana, Pěnčík, Aleš, Ćalić, Dušica, Bogdanović, Milica, Trajković, Milena, Stanišić, Mariana, "New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action" in Frontiers in Plant Science, 13 (2022):875528,
https://doi.org/0.3389/fpls.2022.875528 . .

TY  - BOOK
AU  - Nešković, Mirjana
AU  - Ninković, Slavica
AU  - Miljuš Đukić, Jovanka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5028
AB  - U ovoj publikaciji je iznet presek trenutnog znanja o genetički modifikovanim biljkama. Govoreći o biologiji genetičkih modifikacija uz povezivanje sa procesom evolucije živog sveta, autori daju potencijal za jasnije sagledavanje pitanja genetički modifikovanih biljaka i formiranje kritičkog mišljenja o toj temi, kako kod stručne zajednice tako i kod zainteresovane javnosti. Jasna i koncizna prezentacija doprinosi da knjiga bude podjednako interesantna i razumljiva svima.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
T1  - Genetički modifikovane biljke : biološke osnove – biotehnološke perspektive
SP  - 1
EP  - 406
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5028
ER  - 

@book{
author = "Nešković, Mirjana and Ninković, Slavica and Miljuš Đukić, Jovanka",
year = "2022",
abstract = "U ovoj publikaciji je iznet presek trenutnog znanja o genetički modifikovanim biljkama. Govoreći o biologiji genetičkih modifikacija uz povezivanje sa procesom evolucije živog sveta, autori daju potencijal za jasnije sagledavanje pitanja genetički modifikovanih biljaka i formiranje kritičkog mišljenja o toj temi, kako kod stručne zajednice tako i kod zainteresovane javnosti. Jasna i koncizna prezentacija doprinosi da knjiga bude podjednako interesantna i razumljiva svima.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
title = "Genetički modifikovane biljke : biološke osnove – biotehnološke perspektive",
pages = "1-406",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5028"
}

Nešković, M., Ninković, S.,& Miljuš Đukić, J.. (2022). Genetički modifikovane biljke : biološke osnove – biotehnološke perspektive. 
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 1-406.
https://hdl.handle.net/21.15107/rcub_ibiss_5028

Nešković M, Ninković S, Miljuš Đukić J. Genetički modifikovane biljke : biološke osnove – biotehnološke perspektive. 2022;:1-406.
https://hdl.handle.net/21.15107/rcub_ibiss_5028 .

Nešković, Mirjana, Ninković, Slavica, Miljuš Đukić, Jovanka, "Genetički modifikovane biljke : biološke osnove – biotehnološke perspektive" (2022):1-406,
https://hdl.handle.net/21.15107/rcub_ibiss_5028 .

TY  - JOUR
AU  - Stupar, Sofija
AU  - Dragićević, Milan
AU  - Tešević, Vele
AU  - Stanković-Jeremić, Jovana
AU  - Maksimović, Vuk
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Cingel, Aleksandar
AU  - Vinterhalter, Branka
AU  - Ninković, Slavica
AU  - Savić, Jelena
PY  - 2021
UR  - internal-pdf://Stupar et al. - 2021 - Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf St.pdf
UR  - https://www.mdpi.com/2223-7747/10/1/172
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4147
AB  - Flower strips of French Marigold are commonly used pest repellents in potato fields. However, the effect of French Marigold volatiles on potato metabolism, physiology and induced defense is unknown. Thus, a microarray transcriptome analysis was performed to study the effects of French Marigold essential oil (EO) on laboratory-grown potato. After 8 h of exposure to EO, with gas chromatography/mass spectrometry (GC/MS)-detected terpinolene and limonene as dominant compounds, 2796 transcripts were differentially expressed with fold change >2 compared to expression in controls. A slightly higher number of transcripts had suppressed expression (1493 down- vs. 1303 up-regulated). Since transcripts, annotated to different photosynthesis-related processes, were mostly down-regulated, we selected a set of 10 genes involved in the leaf starch metabolism pathway, and validated microarray patterns using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Except for decreased synthesis and induced decomposition of starch granule in leaves, 8 h long EO exposure slightly elevated the accumulation of sucrose compared to glucose and fructose in subjected potato plants. An in vitro feeding bioassay with Colorado potato beetle showed that EO-induced alternations on transcriptional level and in the sugars’ metabolism caused the enhancement of feeding behavior and overall development of the tested larvae. Results of comprehensive analysis of transcriptional responses in potato exposed to French Marigold EO provide a basis for further elucidation of molecular mechanisms underlying eco-physiological interactions in companion planting cropping systems.
PB  - MDPI AG
T2  - Plants
T1  - Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle
IS  - 1
VL  - 10
DO  - 10.3390/plants10010172
SP  - 172
ER  - 

@article{
author = "Stupar, Sofija and Dragićević, Milan and Tešević, Vele and Stanković-Jeremić, Jovana and Maksimović, Vuk and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Cingel, Aleksandar and Vinterhalter, Branka and Ninković, Slavica and Savić, Jelena",
year = "2021",
abstract = "Flower strips of French Marigold are commonly used pest repellents in potato fields. However, the effect of French Marigold volatiles on potato metabolism, physiology and induced defense is unknown. Thus, a microarray transcriptome analysis was performed to study the effects of French Marigold essential oil (EO) on laboratory-grown potato. After 8 h of exposure to EO, with gas chromatography/mass spectrometry (GC/MS)-detected terpinolene and limonene as dominant compounds, 2796 transcripts were differentially expressed with fold change >2 compared to expression in controls. A slightly higher number of transcripts had suppressed expression (1493 down- vs. 1303 up-regulated). Since transcripts, annotated to different photosynthesis-related processes, were mostly down-regulated, we selected a set of 10 genes involved in the leaf starch metabolism pathway, and validated microarray patterns using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Except for decreased synthesis and induced decomposition of starch granule in leaves, 8 h long EO exposure slightly elevated the accumulation of sucrose compared to glucose and fructose in subjected potato plants. An in vitro feeding bioassay with Colorado potato beetle showed that EO-induced alternations on transcriptional level and in the sugars’ metabolism caused the enhancement of feeding behavior and overall development of the tested larvae. Results of comprehensive analysis of transcriptional responses in potato exposed to French Marigold EO provide a basis for further elucidation of molecular mechanisms underlying eco-physiological interactions in companion planting cropping systems.",
publisher = "MDPI AG",
journal = "Plants",
title = "Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle",
number = "1",
volume = "10",
doi = "10.3390/plants10010172",
pages = "172"
}

Stupar, S., Dragićević, M., Tešević, V., Stanković-Jeremić, J., Maksimović, V., Ćosić, T., Devrnja, N., Tubić, L., Cingel, A., Vinterhalter, B., Ninković, S.,& Savić, J.. (2021). Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle. in Plants
MDPI AG., 10(1), 172.
https://doi.org/10.3390/plants10010172

Stupar S, Dragićević M, Tešević V, Stanković-Jeremić J, Maksimović V, Ćosić T, Devrnja N, Tubić L, Cingel A, Vinterhalter B, Ninković S, Savić J. Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle. in Plants. 2021;10(1):172.
doi:10.3390/plants10010172 .

Stupar, Sofija, Dragićević, Milan, Tešević, Vele, Stanković-Jeremić, Jovana, Maksimović, Vuk, Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Cingel, Aleksandar, Vinterhalter, Branka, Ninković, Slavica, Savić, Jelena, "Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle" in Plants, 10, no. 1 (2021):172,
https://doi.org/10.3390/plants10010172 . .

TY  - JOUR
AU  - Ćosić, Tatjana
AU  - Motyka, Václav
AU  - Savić, Jelena
AU  - Raspor, Martin
AU  - Marković, Marija
AU  - Dobrev, Petre I.
AU  - Ninković, Slavica
PY  - 2021
UR  - https://www.nature.com/articles/s41598-021-85932-w
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4194
AB  - Cross-talk between phytohormones and sugars is intensely involved in plant metabolism, growth and regeneration. We documented alterations in cytokinin (CK) homeostasis in four developmental stages during de novo shoot organogenesis (DNSO) of kohlrabi ( Brassica oleracea var. gongylodes cv. Vienna Purple) seedlings induced by exogenous CKs, trans -zeatin ( trans Z) and thidiazuron (TDZ), added together with elevated sucrose concentration (6% and 9%). Significant impact of CK and sucrose treatment and their interaction was recorded in all investigated stages, including plantlet development before calli formation (T1 and T2), calli formation (T3) and shoot regeneration (T4). Results showed remarkable increase in total CK levels for trans Z treatment, particularly with 9% sucrose. This trend was observed for all physiological and structural groups of CKs. Application of TDZ contributed to little or no increase in CK levels regardless of sucrose concentration. Analysis of expression profiles of organogenesis-related genes involved in auxin transport, CK response, shoot apical meristem formation and cell division revealed that higher sugar concentration significantly downregulated the analysed genes, particularly in T3. This continued on TDZ, but trans Z induced an opposite effect with 9% sucrose in T4, increasing gene activity. Our results demonstrated that phytohormone metabolism might be triggered by sucrose signalling in kohlrabi DNSO.
PB  - Springer Science and Business Media LLC
T2  - Scientific Reports
T1  - Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi
IS  - 1
VL  - 11
DO  - 10.1038/s41598-021-85932-w
SP  - 6494
ER  - 

@article{
author = "Ćosić, Tatjana and Motyka, Václav and Savić, Jelena and Raspor, Martin and Marković, Marija and Dobrev, Petre I. and Ninković, Slavica",
year = "2021",
abstract = "Cross-talk between phytohormones and sugars is intensely involved in plant metabolism, growth and regeneration. We documented alterations in cytokinin (CK) homeostasis in four developmental stages during de novo shoot organogenesis (DNSO) of kohlrabi ( Brassica oleracea var. gongylodes cv. Vienna Purple) seedlings induced by exogenous CKs, trans -zeatin ( trans Z) and thidiazuron (TDZ), added together with elevated sucrose concentration (6% and 9%). Significant impact of CK and sucrose treatment and their interaction was recorded in all investigated stages, including plantlet development before calli formation (T1 and T2), calli formation (T3) and shoot regeneration (T4). Results showed remarkable increase in total CK levels for trans Z treatment, particularly with 9% sucrose. This trend was observed for all physiological and structural groups of CKs. Application of TDZ contributed to little or no increase in CK levels regardless of sucrose concentration. Analysis of expression profiles of organogenesis-related genes involved in auxin transport, CK response, shoot apical meristem formation and cell division revealed that higher sugar concentration significantly downregulated the analysed genes, particularly in T3. This continued on TDZ, but trans Z induced an opposite effect with 9% sucrose in T4, increasing gene activity. Our results demonstrated that phytohormone metabolism might be triggered by sucrose signalling in kohlrabi DNSO.",
publisher = "Springer Science and Business Media LLC",
journal = "Scientific Reports",
title = "Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi",
number = "1",
volume = "11",
doi = "10.1038/s41598-021-85932-w",
pages = "6494"
}

Ćosić, T., Motyka, V., Savić, J., Raspor, M., Marković, M., Dobrev, P. I.,& Ninković, S.. (2021). Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi. in Scientific Reports
Springer Science and Business Media LLC., 11(1), 6494.
https://doi.org/10.1038/s41598-021-85932-w

Ćosić T, Motyka V, Savić J, Raspor M, Marković M, Dobrev PI, Ninković S. Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi. in Scientific Reports. 2021;11(1):6494.
doi:10.1038/s41598-021-85932-w .

Ćosić, Tatjana, Motyka, Václav, Savić, Jelena, Raspor, Martin, Marković, Marija, Dobrev, Petre I., Ninković, Slavica, "Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi" in Scientific Reports, 11, no. 1 (2021):6494,
https://doi.org/10.1038/s41598-021-85932-w . .

TY  - JOUR
AU  - Raspor, Martin
AU  - Motyka, Václav
AU  - Kaleri, Abdul Rasheed
AU  - Ninković, Slavica
AU  - Tubić, Ljiljana
AU  - Cingel, Aleksandar
AU  - Ćosić, Tatjana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4303
AB  - De novo shoot organogenesis (DNSO) is a procedure commonly used for the in vitro
regeneration of shoots from a variety of plant tissues. Shoot regeneration occurs on nutrient media
supplemented with the plant hormones cytokinin (CK) and auxin, which play essential roles in this
process, and genes involved in their signaling cascades act as master regulators of the different phases
of shoot regeneration. In the last 20 years, the genetic regulation of DNSO has been characterized in
detail. However, as of today, the CK and auxin signaling events associated with shoot regeneration
are often interpreted as a consequence of these hormones simply being present in the regeneration
media, whereas the roles for their prior uptake and transport into the cultivated plant tissues are
generally overlooked. Additionally, sucrose, commonly added to the regeneration media as a carbon
source, plays a signaling role and has been recently shown to interact with CK and auxin and to
affect the efficiency of shoot regeneration. In this review, we provide an integrative interpretation
of the roles for CK and auxin in the process of DNSO, adding emphasis on their uptake from the
regeneration media and their interaction with sucrose present in the media to their complex signaling
outputs that mediate shoot regeneration.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs
IS  - 16
VL  - 22
DO  - 10.3390/ijms22168554
SP  - 8554
ER  - 

@article{
author = "Raspor, Martin and Motyka, Václav and Kaleri, Abdul Rasheed and Ninković, Slavica and Tubić, Ljiljana and Cingel, Aleksandar and Ćosić, Tatjana",
year = "2021",
abstract = "De novo shoot organogenesis (DNSO) is a procedure commonly used for the in vitro
regeneration of shoots from a variety of plant tissues. Shoot regeneration occurs on nutrient media
supplemented with the plant hormones cytokinin (CK) and auxin, which play essential roles in this
process, and genes involved in their signaling cascades act as master regulators of the different phases
of shoot regeneration. In the last 20 years, the genetic regulation of DNSO has been characterized in
detail. However, as of today, the CK and auxin signaling events associated with shoot regeneration
are often interpreted as a consequence of these hormones simply being present in the regeneration
media, whereas the roles for their prior uptake and transport into the cultivated plant tissues are
generally overlooked. Additionally, sucrose, commonly added to the regeneration media as a carbon
source, plays a signaling role and has been recently shown to interact with CK and auxin and to
affect the efficiency of shoot regeneration. In this review, we provide an integrative interpretation
of the roles for CK and auxin in the process of DNSO, adding emphasis on their uptake from the
regeneration media and their interaction with sucrose present in the media to their complex signaling
outputs that mediate shoot regeneration.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs",
number = "16",
volume = "22",
doi = "10.3390/ijms22168554",
pages = "8554"
}

Raspor, M., Motyka, V., Kaleri, A. R., Ninković, S., Tubić, L., Cingel, A.,& Ćosić, T.. (2021). Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs. in International Journal of Molecular Sciences
Basel: MDPI., 22(16), 8554.
https://doi.org/10.3390/ijms22168554

Raspor M, Motyka V, Kaleri AR, Ninković S, Tubić L, Cingel A, Ćosić T. Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs. in International Journal of Molecular Sciences. 2021;22(16):8554.
doi:10.3390/ijms22168554 .

Raspor, Martin, Motyka, Václav, Kaleri, Abdul Rasheed, Ninković, Slavica, Tubić, Ljiljana, Cingel, Aleksandar, Ćosić, Tatjana, "Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs" in International Journal of Molecular Sciences, 22, no. 16 (2021):8554,
https://doi.org/10.3390/ijms22168554 . .

TY  - JOUR
AU  - Raspor, Martin
AU  - Motyka, Václav
AU  - Ninković, Slavica
AU  - Malbeck, Jiří
AU  - Dobrev, Petre I.
AU  - Zdravković-Korać, Snežana
AU  - Simonović, Ana
AU  - Ćosić, Tatjana
AU  - Cingel, Aleksandar
AU  - Savić, Jelena
AU  - Zahajská, Lenka
AU  - Tadić, Vojin
AU  - Dragićević, Ivana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3605
AB  - Over the last years, cytokinin deficiency has been studied in a variety of plant species, using transgenic expression of cytokinin oxidase/dehydrogenase genes (CKX). In tobacco and Arabidopsis thaliana, overexpression of A. thaliana CKX genes caused changes in plant morphology known as the "cytokinin deficiency syndrome", including stunted shoot growth, decreased apical dominance, and enhanced root growth. For a valid comparison with the previously obtained in vitro grown AtCKX2-transgenic potato lines, we report here on the construction of transgenic lines of the cultivar Désirée constitutively expressing the gene AtCKX1 under control of the CaMV 35S promoter. Three lines expressing the transgene in both shoot and root tissues were used for analysis. Analyses of CKX activity of tissue extracts, cytokinin levels, morphology, and tuberization parameters revealed similarities as well as differences from the AtCKX2-transgenic plants, including lower levels of CKX activity, and more pronounced morphological effects of cytokinin deficiency. One of the three AtCKX1-overexpressing potato lines was able to start forming tubers before 30 days of growth in long day conditions (16 h/8 h photoperiod) as has been previously observed in some AtCKX2 overexpressors. The extension of the experimental period to 120 days of growth in vitro showed that the other two AtCKX1-overexpressing lines showed impaired tuberization in terms of the number of tubers per plant, and tuber size. We conclude that cytokinin deficiency has distinct effects on tuber induction and tuber initiation/growth, possibly mediated by distinct signaling mechanisms.
T2  - Journal of Plant Growth Regulation
T1  - Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization
VL  - 40
DO  - 10.1007/s00344-020-10080-w
SP  - 37
EP  - 47
ER  - 

@article{
author = "Raspor, Martin and Motyka, Václav and Ninković, Slavica and Malbeck, Jiří and Dobrev, Petre I. and Zdravković-Korać, Snežana and Simonović, Ana and Ćosić, Tatjana and Cingel, Aleksandar and Savić, Jelena and Zahajská, Lenka and Tadić, Vojin and Dragićević, Ivana",
year = "2021",
abstract = "Over the last years, cytokinin deficiency has been studied in a variety of plant species, using transgenic expression of cytokinin oxidase/dehydrogenase genes (CKX). In tobacco and Arabidopsis thaliana, overexpression of A. thaliana CKX genes caused changes in plant morphology known as the "cytokinin deficiency syndrome", including stunted shoot growth, decreased apical dominance, and enhanced root growth. For a valid comparison with the previously obtained in vitro grown AtCKX2-transgenic potato lines, we report here on the construction of transgenic lines of the cultivar Désirée constitutively expressing the gene AtCKX1 under control of the CaMV 35S promoter. Three lines expressing the transgene in both shoot and root tissues were used for analysis. Analyses of CKX activity of tissue extracts, cytokinin levels, morphology, and tuberization parameters revealed similarities as well as differences from the AtCKX2-transgenic plants, including lower levels of CKX activity, and more pronounced morphological effects of cytokinin deficiency. One of the three AtCKX1-overexpressing potato lines was able to start forming tubers before 30 days of growth in long day conditions (16 h/8 h photoperiod) as has been previously observed in some AtCKX2 overexpressors. The extension of the experimental period to 120 days of growth in vitro showed that the other two AtCKX1-overexpressing lines showed impaired tuberization in terms of the number of tubers per plant, and tuber size. We conclude that cytokinin deficiency has distinct effects on tuber induction and tuber initiation/growth, possibly mediated by distinct signaling mechanisms.",
journal = "Journal of Plant Growth Regulation",
title = "Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization",
volume = "40",
doi = "10.1007/s00344-020-10080-w",
pages = "37-47"
}

Raspor, M., Motyka, V., Ninković, S., Malbeck, J., Dobrev, P. I., Zdravković-Korać, S., Simonović, A., Ćosić, T., Cingel, A., Savić, J., Zahajská, L., Tadić, V.,& Dragićević, I.. (2021). Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization. in Journal of Plant Growth Regulation, 40, 37-47.
https://doi.org/10.1007/s00344-020-10080-w

Raspor M, Motyka V, Ninković S, Malbeck J, Dobrev PI, Zdravković-Korać S, Simonović A, Ćosić T, Cingel A, Savić J, Zahajská L, Tadić V, Dragićević I. Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization. in Journal of Plant Growth Regulation. 2021;40:37-47.
doi:10.1007/s00344-020-10080-w .

Raspor, Martin, Motyka, Václav, Ninković, Slavica, Malbeck, Jiří, Dobrev, Petre I., Zdravković-Korać, Snežana, Simonović, Ana, Ćosić, Tatjana, Cingel, Aleksandar, Savić, Jelena, Zahajská, Lenka, Tadić, Vojin, Dragićević, Ivana, "Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization" in Journal of Plant Growth Regulation, 40 (2021):37-47,
https://doi.org/10.1007/s00344-020-10080-w . .

TY  - JOUR
AU  - Ćosić, Tatjana
AU  - Savić, Jelena
AU  - Raspor, Martin
AU  - Cingel, Aleksandar
AU  - Ghalawnji, Nabil
AU  - Vinterhalter, Branka
AU  - Ninković, Slavica
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3816
AB  - Kohlrabi (Brassica oleracea var. gongylodes), with its edible stem tuber formed at the base of
the plant stem, presents a valuable source of nutrients. The potential effects of plant growth regulators
(PGRs), as well as various concentrations of different sugars on the in vitro development of kohlrabi were
studied. Ten-day-old kohlrabi seedlings were cultivated in vitro for 5 weeks at 18±2°C on half-strength
MS media containing different concentrations of carbon source such as sucrose, fructose, glucose, xylose
and mannitol, combined with or without specific plant growth regulators (N6-benzyladenine (BA),
gibberellic acid (GA3), 2,3,5-triiodobenzoic acid (TIBA)). Results showed no tuber formation in all
treatments, but growth and development of treated kohlrabi seedlings was significantly affected in a
distinctive manner, with a variety of morphological traits being altered in comparison to matching
controls.
PB  - Serbian Biological Socitey
T2  - Archives of Biological Sciences
T1  - Effects of different types of sugars and plant growth regulators on kohlrabi seedling growth and development in vitro
IS  - 3
VL  - 72
DO  - 10.2298/ABS200622029C
SP  - 349
EP  - 357
ER  - 

@article{
author = "Ćosić, Tatjana and Savić, Jelena and Raspor, Martin and Cingel, Aleksandar and Ghalawnji, Nabil and Vinterhalter, Branka and Ninković, Slavica",
year = "2020",
abstract = "Kohlrabi (Brassica oleracea var. gongylodes), with its edible stem tuber formed at the base of
the plant stem, presents a valuable source of nutrients. The potential effects of plant growth regulators
(PGRs), as well as various concentrations of different sugars on the in vitro development of kohlrabi were
studied. Ten-day-old kohlrabi seedlings were cultivated in vitro for 5 weeks at 18±2°C on half-strength
MS media containing different concentrations of carbon source such as sucrose, fructose, glucose, xylose
and mannitol, combined with or without specific plant growth regulators (N6-benzyladenine (BA),
gibberellic acid (GA3), 2,3,5-triiodobenzoic acid (TIBA)). Results showed no tuber formation in all
treatments, but growth and development of treated kohlrabi seedlings was significantly affected in a
distinctive manner, with a variety of morphological traits being altered in comparison to matching
controls.",
publisher = "Serbian Biological Socitey",
journal = "Archives of Biological Sciences",
title = "Effects of different types of sugars and plant growth regulators on kohlrabi seedling growth and development in vitro",
number = "3",
volume = "72",
doi = "10.2298/ABS200622029C",
pages = "349-357"
}

Ćosić, T., Savić, J., Raspor, M., Cingel, A., Ghalawnji, N., Vinterhalter, B.,& Ninković, S.. (2020). Effects of different types of sugars and plant growth regulators on kohlrabi seedling growth and development in vitro. in Archives of Biological Sciences
Serbian Biological Socitey., 72(3), 349-357.
https://doi.org/10.2298/ABS200622029C

Ćosić T, Savić J, Raspor M, Cingel A, Ghalawnji N, Vinterhalter B, Ninković S. Effects of different types of sugars and plant growth regulators on kohlrabi seedling growth and development in vitro. in Archives of Biological Sciences. 2020;72(3):349-357.
doi:10.2298/ABS200622029C .

Ćosić, Tatjana, Savić, Jelena, Raspor, Martin, Cingel, Aleksandar, Ghalawnji, Nabil, Vinterhalter, Branka, Ninković, Slavica, "Effects of different types of sugars and plant growth regulators on kohlrabi seedling growth and development in vitro" in Archives of Biological Sciences, 72, no. 3 (2020):349-357,
https://doi.org/10.2298/ABS200622029C . .

TY  - JOUR
AU  - Raspor, Martin
AU  - Motyka, Václav
AU  - Ninković, Slavica
AU  - Dobrev, Petre I.
AU  - Malbeck, Jiří
AU  - Ćosić, Tatjana
AU  - Cingel, Aleksandar
AU  - Savić, Jelena
AU  - Tadić, Vojin
AU  - Dragićević, Ivana
PY  - 2020
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC7044434
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3623
AB  - A number of scientific reports published to date contain data on endogenous levels of various phytohormones in potato (Solanum tuberosum L.) but a complete cytokinin profile of potato tissues, that would include data on all particular molecular forms of cytokinin, has still been missing. In this work, endogenous levels of all analytically detectable isoprenoid cytokinins, as well as the auxin indole-3-acetic acid (IAA), and abscisic acid (ABA) have been determined in shoots and roots of 30 day old in vitro grown potato (cv. Désirée). The results presented here are generally similar to other data reported for in vitro grown potato plants, whereas greenhouse-grown plants typically contain lower levels of ABA, possibly indicating that in vitro grown potato is exposed to chronic stress. Cytokinin N-glucosides, particularly N7-glucosides, are the dominant cytokinin forms in both shoots and roots of potato, whereas nucleobases, as the bioactive forms of cytokinins, comprise a low proportion of cytokinin levels in tissues of potato. Differences in phytohormone composition between shoots and roots of potato suggest specific patterns of transport and/or differences in tissue-specific metabolism of plant hormones. These results represent a contribution to understanding the hormonomics of potato, a crop species of extraordinary economic importance.
T2  - Scientific Reports
T1  - Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.
IS  - 1
VL  - 10
DO  - 10.1038/s41598-020-60412-9
SP  - 3437
ER  - 

@article{
author = "Raspor, Martin and Motyka, Václav and Ninković, Slavica and Dobrev, Petre I. and Malbeck, Jiří and Ćosić, Tatjana and Cingel, Aleksandar and Savić, Jelena and Tadić, Vojin and Dragićević, Ivana",
year = "2020",
abstract = "A number of scientific reports published to date contain data on endogenous levels of various phytohormones in potato (Solanum tuberosum L.) but a complete cytokinin profile of potato tissues, that would include data on all particular molecular forms of cytokinin, has still been missing. In this work, endogenous levels of all analytically detectable isoprenoid cytokinins, as well as the auxin indole-3-acetic acid (IAA), and abscisic acid (ABA) have been determined in shoots and roots of 30 day old in vitro grown potato (cv. Désirée). The results presented here are generally similar to other data reported for in vitro grown potato plants, whereas greenhouse-grown plants typically contain lower levels of ABA, possibly indicating that in vitro grown potato is exposed to chronic stress. Cytokinin N-glucosides, particularly N7-glucosides, are the dominant cytokinin forms in both shoots and roots of potato, whereas nucleobases, as the bioactive forms of cytokinins, comprise a low proportion of cytokinin levels in tissues of potato. Differences in phytohormone composition between shoots and roots of potato suggest specific patterns of transport and/or differences in tissue-specific metabolism of plant hormones. These results represent a contribution to understanding the hormonomics of potato, a crop species of extraordinary economic importance.",
journal = "Scientific Reports",
title = "Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.",
number = "1",
volume = "10",
doi = "10.1038/s41598-020-60412-9",
pages = "3437"
}

Raspor, M., Motyka, V., Ninković, S., Dobrev, P. I., Malbeck, J., Ćosić, T., Cingel, A., Savić, J., Tadić, V.,& Dragićević, I.. (2020). Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.. in Scientific Reports, 10(1), 3437.
https://doi.org/10.1038/s41598-020-60412-9

Raspor M, Motyka V, Ninković S, Dobrev PI, Malbeck J, Ćosić T, Cingel A, Savić J, Tadić V, Dragićević I. Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.. in Scientific Reports. 2020;10(1):3437.
doi:10.1038/s41598-020-60412-9 .

Raspor, Martin, Motyka, Václav, Ninković, Slavica, Dobrev, Petre I., Malbeck, Jiří, Ćosić, Tatjana, Cingel, Aleksandar, Savić, Jelena, Tadić, Vojin, Dragićević, Ivana, "Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics." in Scientific Reports, 10, no. 1 (2020):3437,
https://doi.org/10.1038/s41598-020-60412-9 . .

TY  - JOUR
AU  - Ćosić, Tatjana
AU  - Raspor, Martin
AU  - Savić, Jelena
AU  - Cingel, Aleksandar
AU  - Matekalo, Dragana
AU  - Zdravković-Korać, Snežana
AU  - Ninković, Slavica
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0176161718304140?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3213
AB  - Kohlrabi (Brassica oleracea var. gongylodes) is an important vegetable crop that is able to undergo shoot regeneration in culture from intact seedlings in a single-step regeneration process, using cytokinin as the only plant growth regulator. In this work, we present the expression profiles of seven organogenesis-related genes over the time course of shoot regeneration from intact seedlings of kohlrabi cv. Vienna Purple on shoot regeneration media containing trans-zeatin, cis-zeatin, benzyl adenine or thidiazuron. Two auxin transporter genes - PIN3 and PIN4, a cytokinin response regulator - ARR5, two shoot apical meristem-related transcription factors - CUC1 and RGD3, and two cell cycle-related genes - CDKB2;1 and CYCB2;4 - displayed bimodal expression patterns on most cytokinin-containing media when their expression levels were normalized against control plants grown on hormone-free media. The first expression peak corresponded to direct upregulation by cytokinin from the growth media, and the second one reflected transcriptional events related to callus formation and/or acquisition of organogenic competence, corresponding to the shoot regeneration phases that have already been characterized in Arabidopsis thaliana. We demonstrate that the genes involved in the two-step shoot regeneration of Arabidopsis display their expected expression profiles during the single-step shoot regeneration of its close phylogenetic relative kohlrabi confirming the universality of their roles in the distinct phases of the regeneration process in Brassicaceae. The results presented here represent a first step towards genetic characterization of the morphogenetic processes in this important crop species.
T2  - Journal of Plant Physiology
T1  - Expression profiles of organogenesis-related genes over the time course of one-step de novo shoot organogenesis from intact seedlings of kohlrabi.
VL  - 232
DO  - 10.1016/j.jplph.2018.11.004
SP  - 257
EP  - 269
ER  - 

@article{
author = "Ćosić, Tatjana and Raspor, Martin and Savić, Jelena and Cingel, Aleksandar and Matekalo, Dragana and Zdravković-Korać, Snežana and Ninković, Slavica",
year = "2019",
abstract = "Kohlrabi (Brassica oleracea var. gongylodes) is an important vegetable crop that is able to undergo shoot regeneration in culture from intact seedlings in a single-step regeneration process, using cytokinin as the only plant growth regulator. In this work, we present the expression profiles of seven organogenesis-related genes over the time course of shoot regeneration from intact seedlings of kohlrabi cv. Vienna Purple on shoot regeneration media containing trans-zeatin, cis-zeatin, benzyl adenine or thidiazuron. Two auxin transporter genes - PIN3 and PIN4, a cytokinin response regulator - ARR5, two shoot apical meristem-related transcription factors - CUC1 and RGD3, and two cell cycle-related genes - CDKB2;1 and CYCB2;4 - displayed bimodal expression patterns on most cytokinin-containing media when their expression levels were normalized against control plants grown on hormone-free media. The first expression peak corresponded to direct upregulation by cytokinin from the growth media, and the second one reflected transcriptional events related to callus formation and/or acquisition of organogenic competence, corresponding to the shoot regeneration phases that have already been characterized in Arabidopsis thaliana. We demonstrate that the genes involved in the two-step shoot regeneration of Arabidopsis display their expected expression profiles during the single-step shoot regeneration of its close phylogenetic relative kohlrabi confirming the universality of their roles in the distinct phases of the regeneration process in Brassicaceae. The results presented here represent a first step towards genetic characterization of the morphogenetic processes in this important crop species.",
journal = "Journal of Plant Physiology",
title = "Expression profiles of organogenesis-related genes over the time course of one-step de novo shoot organogenesis from intact seedlings of kohlrabi.",
volume = "232",
doi = "10.1016/j.jplph.2018.11.004",
pages = "257-269"
}

Ćosić, T., Raspor, M., Savić, J., Cingel, A., Matekalo, D., Zdravković-Korać, S.,& Ninković, S.. (2019). Expression profiles of organogenesis-related genes over the time course of one-step de novo shoot organogenesis from intact seedlings of kohlrabi.. in Journal of Plant Physiology, 232, 257-269.
https://doi.org/10.1016/j.jplph.2018.11.004

Ćosić T, Raspor M, Savić J, Cingel A, Matekalo D, Zdravković-Korać S, Ninković S. Expression profiles of organogenesis-related genes over the time course of one-step de novo shoot organogenesis from intact seedlings of kohlrabi.. in Journal of Plant Physiology. 2019;232:257-269.
doi:10.1016/j.jplph.2018.11.004 .

Ćosić, Tatjana, Raspor, Martin, Savić, Jelena, Cingel, Aleksandar, Matekalo, Dragana, Zdravković-Korać, Snežana, Ninković, Slavica, "Expression profiles of organogenesis-related genes over the time course of one-step de novo shoot organogenesis from intact seedlings of kohlrabi." in Journal of Plant Physiology, 232 (2019):257-269,
https://doi.org/10.1016/j.jplph.2018.11.004 . .

TY  - CONF
AU  - Ćosić, Tatjana
AU  - Motyka, Vaclav
AU  - Savić, Jelena
AU  - Cingel, Aleksandar
AU  - Raspor, Martin
AU  - Devrnja, Nina
AU  - Dobrev, Petre I.
AU  - Ninković, Slavica
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6129
AB  - Cross–talk between phytohormones and sugars was earlier shown to be involved in higher plants regeneration. While our previous work revealed that phytohormones metabolism may be triggered by sucrose signalling in de novo shoot organogenesis (DNSO) of kohlrabi (Brassica oleracea var. gongylodes), the effect of sucrose on callus formation and shoot regeneration remains unknown in this important crop. Present study was intended to estimate if successful DNSO, occurring from intact kohlrabi seedlings due to influence of exogenous cytokinins (CKs) - trans zeatin (transZ) and thidiazuron (TDZ) and high sucrose concentration (6 and 9%), was accompanied by distinctive alterations in expression levels of organogenesis-related genes. The relative activity of genes involved in auxin transport, CK response, de novo shoot apical meristem formation and cell division - PIN3, ARR5, RGD3, CDKB2;1 and CYC2;4, was determined by quantitative PCR analysis showing variation in expression levels during callus formation (CF) and de novo shoots regeneration (SR). Generally, expression was downregulated with TDZ being more effective, especially when higher amount of sugar was applied. Exception was ARR5, mainly upregulated with transZ that had greater impact than in other analysed genes. We also examined correlations between total CK content and distinct gene expression depending on the treatment. Our results demonstrated predominantly negative correlations, apart from all TDZ treatments in CF and TDZ and transZ with 9% sucrose in SR, indicating complex regulatory network underlying kohlrabi DNSO.
PB  - Olomouc: Europian Federation of Biotechnology
C3  - Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic
T1  - Interaction of exogenous cytokinins and sucrose affects expression of organogenesis-related genes in kohlrabi in vitro regeneration
SP  - 131
EP  - 131
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6129
ER  - 

@conference{
author = "Ćosić, Tatjana and Motyka, Vaclav and Savić, Jelena and Cingel, Aleksandar and Raspor, Martin and Devrnja, Nina and Dobrev, Petre I. and Ninković, Slavica",
year = "2019",
abstract = "Cross–talk between phytohormones and sugars was earlier shown to be involved in higher plants regeneration. While our previous work revealed that phytohormones metabolism may be triggered by sucrose signalling in de novo shoot organogenesis (DNSO) of kohlrabi (Brassica oleracea var. gongylodes), the effect of sucrose on callus formation and shoot regeneration remains unknown in this important crop. Present study was intended to estimate if successful DNSO, occurring from intact kohlrabi seedlings due to influence of exogenous cytokinins (CKs) - trans zeatin (transZ) and thidiazuron (TDZ) and high sucrose concentration (6 and 9%), was accompanied by distinctive alterations in expression levels of organogenesis-related genes. The relative activity of genes involved in auxin transport, CK response, de novo shoot apical meristem formation and cell division - PIN3, ARR5, RGD3, CDKB2;1 and CYC2;4, was determined by quantitative PCR analysis showing variation in expression levels during callus formation (CF) and de novo shoots regeneration (SR). Generally, expression was downregulated with TDZ being more effective, especially when higher amount of sugar was applied. Exception was ARR5, mainly upregulated with transZ that had greater impact than in other analysed genes. We also examined correlations between total CK content and distinct gene expression depending on the treatment. Our results demonstrated predominantly negative correlations, apart from all TDZ treatments in CF and TDZ and transZ with 9% sucrose in SR, indicating complex regulatory network underlying kohlrabi DNSO.",
publisher = "Olomouc: Europian Federation of Biotechnology",
journal = "Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic",
title = "Interaction of exogenous cytokinins and sucrose affects expression of organogenesis-related genes in kohlrabi in vitro regeneration",
pages = "131-131",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6129"
}

Ćosić, T., Motyka, V., Savić, J., Cingel, A., Raspor, M., Devrnja, N., Dobrev, P. I.,& Ninković, S.. (2019). Interaction of exogenous cytokinins and sucrose affects expression of organogenesis-related genes in kohlrabi in vitro regeneration. in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic
Olomouc: Europian Federation of Biotechnology., 131-131.
https://hdl.handle.net/21.15107/rcub_ibiss_6129

Ćosić T, Motyka V, Savić J, Cingel A, Raspor M, Devrnja N, Dobrev PI, Ninković S. Interaction of exogenous cytokinins and sucrose affects expression of organogenesis-related genes in kohlrabi in vitro regeneration. in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic. 2019;:131-131.
https://hdl.handle.net/21.15107/rcub_ibiss_6129 .

Ćosić, Tatjana, Motyka, Vaclav, Savić, Jelena, Cingel, Aleksandar, Raspor, Martin, Devrnja, Nina, Dobrev, Petre I., Ninković, Slavica, "Interaction of exogenous cytokinins and sucrose affects expression of organogenesis-related genes in kohlrabi in vitro regeneration" in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic (2019):131-131,
https://hdl.handle.net/21.15107/rcub_ibiss_6129 .

TY  - CONF
AU  - Stupar, Sofija
AU  - Avramović, Miloš
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Cingel, Aleksandar
AU  - Ninković, Slavica
AU  - Vinterhalter, Branka
AU  - Savić, Jelena
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5593
AB  - Potato fields pest protection is still predominantly based on synthetic pesticides, expensive in terms of monetary investment and proved to be harmful to human health and the environment. Alternatively, flower strips of French marigold (Tagetes patula L.) appearing as potent pest repellents in potato fields, offer well-adopted practice in organic farming. Up to now, the effect of French marigoldon potato metabolism, physiology, and yield has not been studied in detail. Thus, a microarray transcriptome analysis was performed to study the effect of French marigold essential oil (EO) on laboratory-grown potato, with the focus on starch metabolism pathway. After 8h of exposure to EO, 3840 potato probes were differentially expressed (fold change ≥2) compared to control plants, of which 1744 were upregulated and 2096 downregulated. We selected a set of putative genes involved in the starch metabolism pathway in plastids, and validated microarray patterns using RT-qPCR method. The expression of selected genes was analyzed in plants exposed to EO for 4, 8 and 12h . While the expression of genes involved in starch biosynthesis was at similar levels as in non-treated plants, the most of the genes responsible for gradual degradation of starch displayed significantly elevated expression with the highest levels detected after 8 and 12h of exposure. Our results provide a comprehensive sequence resource for studying the potato-EO interactions, revealing induced alternations in potato starch metabolism and suggesting modified regulatory mechanism in potato affected by EO.
PB  - European Federation of Biotechnology
C3  - Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic
T1  - Transcriptional profiling of starch metabolism in potato plants exposed to French marigold essential oil
SP  - 129
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5593
ER  - 

@conference{
author = "Stupar, Sofija and Avramović, Miloš and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Cingel, Aleksandar and Ninković, Slavica and Vinterhalter, Branka and Savić, Jelena",
year = "2019",
abstract = "Potato fields pest protection is still predominantly based on synthetic pesticides, expensive in terms of monetary investment and proved to be harmful to human health and the environment. Alternatively, flower strips of French marigold (Tagetes patula L.) appearing as potent pest repellents in potato fields, offer well-adopted practice in organic farming. Up to now, the effect of French marigoldon potato metabolism, physiology, and yield has not been studied in detail. Thus, a microarray transcriptome analysis was performed to study the effect of French marigold essential oil (EO) on laboratory-grown potato, with the focus on starch metabolism pathway. After 8h of exposure to EO, 3840 potato probes were differentially expressed (fold change ≥2) compared to control plants, of which 1744 were upregulated and 2096 downregulated. We selected a set of putative genes involved in the starch metabolism pathway in plastids, and validated microarray patterns using RT-qPCR method. The expression of selected genes was analyzed in plants exposed to EO for 4, 8 and 12h . While the expression of genes involved in starch biosynthesis was at similar levels as in non-treated plants, the most of the genes responsible for gradual degradation of starch displayed significantly elevated expression with the highest levels detected after 8 and 12h of exposure. Our results provide a comprehensive sequence resource for studying the potato-EO interactions, revealing induced alternations in potato starch metabolism and suggesting modified regulatory mechanism in potato affected by EO.",
publisher = "European Federation of Biotechnology",
journal = "Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic",
title = "Transcriptional profiling of starch metabolism in potato plants exposed to French marigold essential oil",
pages = "129",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5593"
}

Stupar, S., Avramović, M., Ćosić, T., Devrnja, N., Tubić, L., Cingel, A., Ninković, S., Vinterhalter, B.,& Savić, J.. (2019). Transcriptional profiling of starch metabolism in potato plants exposed to French marigold essential oil. in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic
European Federation of Biotechnology., 129.
https://hdl.handle.net/21.15107/rcub_ibiss_5593

Stupar S, Avramović M, Ćosić T, Devrnja N, Tubić L, Cingel A, Ninković S, Vinterhalter B, Savić J. Transcriptional profiling of starch metabolism in potato plants exposed to French marigold essential oil. in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic. 2019;:129.
https://hdl.handle.net/21.15107/rcub_ibiss_5593 .

Stupar, Sofija, Avramović, Miloš, Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Cingel, Aleksandar, Ninković, Slavica, Vinterhalter, Branka, Savić, Jelena, "Transcriptional profiling of starch metabolism in potato plants exposed to French marigold essential oil" in Book of abstracts: Plant Biotechnology: Green for Good V; 2019 Jun 10-13; Olomouc, Czech Republic (2019):129,
https://hdl.handle.net/21.15107/rcub_ibiss_5593 .

TY  - JOUR
AU  - Stanišić, Mariana
AU  - Ćosić, Tatjana
AU  - Savić, Jelena
AU  - Krstić Milošević, Dijana
AU  - Mišić, Danijela
AU  - Smigocki, Ann
AU  - Ninković, Slavica
AU  - Banjac, Nevena
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3791
AB  - Allelopathic plants exploit their chemical ‘weapons’ to prevail over the competition, suppress neighboring plants and consequently
use the available resources more efficiently. However, the investigation of plant allelopathic interactions in rhizosphere is difficult
to perform because of its high complexity due to interactions of biotic and abiotic factors. Thus, autonomous, aseptic root cultures
of apple (Malus × domestica Borkh.) could facilitate allelopathic studies. We report on the successful genetic transformation of
apple cultivars Melrose, Golden Delicious, Cadel and Gloster using ˇ Agrobacterium rhizogenes (Riker et al. 1930) Conn 1942 strain
15834 and for the first time the establishment of apple autonomous and permanent in vitro hairy root cultures that could be used
as a new tool for apple allelopathic assays. Molecular characterization of transgenic hairy root lines was conducted to elucidate
the possible relationship between expression of T-DNA genes and root growth characteristics that include branching. Similar content of phenolic acids (chlorogenic, caffeic, syringic, p-coumaric and ferulic), glycosilated flavonoids (rutin, quercitrin, isoquercitrin,
kaempferol-3-glucoside) and flavonoid aglycones (quercetin and naringenin), and dihydrochalcone phloridzin, was detected in
untransformed and transgenic apple root tissue by ultra high-performance liquid chromatography with mass spectrometry
(UHPLC/(+/–)HESI–MS/MS) analyses, confirming that genetic transformation did not disturb secondary metabolite production in
apple. Chlorogenic and caffeic acids and dihydrochalcones phloridzin and phloretin were detected as putative allelochemicals
exuded into the growth medium in which transgenic roots were maintained for 4 weeks. Apple hairy root exudates significantly
affected shoot and root development and growth of test plant Arabidopsis thaliana (L.) Heynh. seedlings after 5 or 10 days of
treatment. Additionally, core cell-cycle genes CDKA1;1, CDKB2;1, CYCA3;1 and CYCB2;4 were down regulated in Arabidopsis
shoots suggesting, in part, their role in inhibition of shoot growth. The present work highlighted an autonomous and permanent
in vitro hairy root culture system as a valuable tool for studying allelopathic potential of apple, offering new perspective for allelopathy background elucidation in this important fruit species.
PB  - Oxford:Oxford University Press
T2  - Tree Physiology
T1  - Hairy root culture as a valuable tool for allelopathic studies in apple
IS  - 5
VL  - 39
DO  - doi:10.1093/treephys/tpz006
SP  - 888
EP  - 905
ER  - 

@article{
author = "Stanišić, Mariana and Ćosić, Tatjana and Savić, Jelena and Krstić Milošević, Dijana and Mišić, Danijela and Smigocki, Ann and Ninković, Slavica and Banjac, Nevena",
year = "2019",
abstract = "Allelopathic plants exploit their chemical ‘weapons’ to prevail over the competition, suppress neighboring plants and consequently
use the available resources more efficiently. However, the investigation of plant allelopathic interactions in rhizosphere is difficult
to perform because of its high complexity due to interactions of biotic and abiotic factors. Thus, autonomous, aseptic root cultures
of apple (Malus × domestica Borkh.) could facilitate allelopathic studies. We report on the successful genetic transformation of
apple cultivars Melrose, Golden Delicious, Cadel and Gloster using ˇ Agrobacterium rhizogenes (Riker et al. 1930) Conn 1942 strain
15834 and for the first time the establishment of apple autonomous and permanent in vitro hairy root cultures that could be used
as a new tool for apple allelopathic assays. Molecular characterization of transgenic hairy root lines was conducted to elucidate
the possible relationship between expression of T-DNA genes and root growth characteristics that include branching. Similar content of phenolic acids (chlorogenic, caffeic, syringic, p-coumaric and ferulic), glycosilated flavonoids (rutin, quercitrin, isoquercitrin,
kaempferol-3-glucoside) and flavonoid aglycones (quercetin and naringenin), and dihydrochalcone phloridzin, was detected in
untransformed and transgenic apple root tissue by ultra high-performance liquid chromatography with mass spectrometry
(UHPLC/(+/–)HESI–MS/MS) analyses, confirming that genetic transformation did not disturb secondary metabolite production in
apple. Chlorogenic and caffeic acids and dihydrochalcones phloridzin and phloretin were detected as putative allelochemicals
exuded into the growth medium in which transgenic roots were maintained for 4 weeks. Apple hairy root exudates significantly
affected shoot and root development and growth of test plant Arabidopsis thaliana (L.) Heynh. seedlings after 5 or 10 days of
treatment. Additionally, core cell-cycle genes CDKA1;1, CDKB2;1, CYCA3;1 and CYCB2;4 were down regulated in Arabidopsis
shoots suggesting, in part, their role in inhibition of shoot growth. The present work highlighted an autonomous and permanent
in vitro hairy root culture system as a valuable tool for studying allelopathic potential of apple, offering new perspective for allelopathy background elucidation in this important fruit species.",
publisher = "Oxford:Oxford University Press",
journal = "Tree Physiology",
title = "Hairy root culture as a valuable tool for allelopathic studies in apple",
number = "5",
volume = "39",
doi = "doi:10.1093/treephys/tpz006",
pages = "888-905"
}

Stanišić, M., Ćosić, T., Savić, J., Krstić Milošević, D., Mišić, D., Smigocki, A., Ninković, S.,& Banjac, N.. (2019). Hairy root culture as a valuable tool for allelopathic studies in apple. in Tree Physiology
Oxford:Oxford University Press., 39(5), 888-905.
https://doi.org/doi:10.1093/treephys/tpz006

Stanišić M, Ćosić T, Savić J, Krstić Milošević D, Mišić D, Smigocki A, Ninković S, Banjac N. Hairy root culture as a valuable tool for allelopathic studies in apple. in Tree Physiology. 2019;39(5):888-905.
doi:doi:10.1093/treephys/tpz006 .

Stanišić, Mariana, Ćosić, Tatjana, Savić, Jelena, Krstić Milošević, Dijana, Mišić, Danijela, Smigocki, Ann, Ninković, Slavica, Banjac, Nevena, "Hairy root culture as a valuable tool for allelopathic studies in apple" in Tree Physiology, 39, no. 5 (2019):888-905,
https://doi.org/doi:10.1093/treephys/tpz006 . .

TY  - JOUR
AU  - Stanišić, Mariana
AU  - Ćosić, Tatjana
AU  - Savić, Jelena
AU  - Krstić Milošević, Dijana
AU  - Mišić, Danijela
AU  - Smigocki, Ann
AU  - Ninković, Slavica
AU  - Banjac, Nevena
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3791
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3792
AB  - Allelopathic plants exploit their chemical ‘weapons’ to prevail over the competition, suppress neighboring plants and consequentlyuse the available resources more efficiently. However, the investigation of plant allelopathic interactions in rhizosphere is difficultto perform because of its high complexity due to interactions of biotic and abiotic factors. Thus, autonomous, aseptic root culturesof apple (Malus × domestica Borkh.) could facilitate allelopathic studies. We report on the successful genetic transformation ofapple cultivars Melrose, Golden Delicious, Cadel and Gloster using ˇ Agrobacterium rhizogenes (Riker et al. 1930) Conn 1942 strain15834 and for the first time the establishment of apple autonomous and permanent in vitro hairy root cultures that could be usedas a new tool for apple allelopathic assays. Molecular characterization of transgenic hairy root lines was conducted to elucidatethe possible relationship between expression of T-DNA genes and root growth characteristics that include branching. Similar content of phenolic acids (chlorogenic, caffeic, syringic, p-coumaric and ferulic), glycosilated flavonoids (rutin, quercitrin, isoquercitrin,kaempferol-3-glucoside) and flavonoid aglycones (quercetin and naringenin), and dihydrochalcone phloridzin, was detected inuntransformed and transgenic apple root tissue by ultra high-performance liquid chromatography with mass spectrometry(UHPLC/(+/–)HESI–MS/MS) analyses, confirming that genetic transformation did not disturb secondary metabolite production inapple. Chlorogenic and caffeic acids and dihydrochalcones phloridzin and phloretin were detected as putative allelochemicalsexuded into the growth medium in which transgenic roots were maintained for 4 weeks. Apple hairy root exudates significantlyaffected shoot and root development and growth of test plant Arabidopsis thaliana (L.) Heynh. seedlings after 5 or 10 days oftreatment. Additionally, core cell-cycle genes CDKA1;1, CDKB2;1, CYCA3;1 and CYCB2;4 were down regulated in Arabidopsisshoots suggesting, in part, their role in inhibition of shoot growth. The present work highlighted an autonomous and permanentin vitro hairy root culture system as a valuable tool for studying allelopathic potential of apple, offering new perspective for allelopathy background elucidation in this important fruit species.
PB  - Oxford: Oxford University Press
T2  - Tree Physiology
T1  - Hairy root culture as a valuable tool for allelopathic studies in apple
IS  - 5
VL  - 39
DO  - doi:10.1093/treephys/tpz006
SP  - 888
EP  - 905
ER  - 

@article{
author = "Stanišić, Mariana and Ćosić, Tatjana and Savić, Jelena and Krstić Milošević, Dijana and Mišić, Danijela and Smigocki, Ann and Ninković, Slavica and Banjac, Nevena",
year = "2019",
abstract = "Allelopathic plants exploit their chemical ‘weapons’ to prevail over the competition, suppress neighboring plants and consequentlyuse the available resources more efficiently. However, the investigation of plant allelopathic interactions in rhizosphere is difficultto perform because of its high complexity due to interactions of biotic and abiotic factors. Thus, autonomous, aseptic root culturesof apple (Malus × domestica Borkh.) could facilitate allelopathic studies. We report on the successful genetic transformation ofapple cultivars Melrose, Golden Delicious, Cadel and Gloster using ˇ Agrobacterium rhizogenes (Riker et al. 1930) Conn 1942 strain15834 and for the first time the establishment of apple autonomous and permanent in vitro hairy root cultures that could be usedas a new tool for apple allelopathic assays. Molecular characterization of transgenic hairy root lines was conducted to elucidatethe possible relationship between expression of T-DNA genes and root growth characteristics that include branching. Similar content of phenolic acids (chlorogenic, caffeic, syringic, p-coumaric and ferulic), glycosilated flavonoids (rutin, quercitrin, isoquercitrin,kaempferol-3-glucoside) and flavonoid aglycones (quercetin and naringenin), and dihydrochalcone phloridzin, was detected inuntransformed and transgenic apple root tissue by ultra high-performance liquid chromatography with mass spectrometry(UHPLC/(+/–)HESI–MS/MS) analyses, confirming that genetic transformation did not disturb secondary metabolite production inapple. Chlorogenic and caffeic acids and dihydrochalcones phloridzin and phloretin were detected as putative allelochemicalsexuded into the growth medium in which transgenic roots were maintained for 4 weeks. Apple hairy root exudates significantlyaffected shoot and root development and growth of test plant Arabidopsis thaliana (L.) Heynh. seedlings after 5 or 10 days oftreatment. Additionally, core cell-cycle genes CDKA1;1, CDKB2;1, CYCA3;1 and CYCB2;4 were down regulated in Arabidopsisshoots suggesting, in part, their role in inhibition of shoot growth. The present work highlighted an autonomous and permanentin vitro hairy root culture system as a valuable tool for studying allelopathic potential of apple, offering new perspective for allelopathy background elucidation in this important fruit species.",
publisher = "Oxford: Oxford University Press",
journal = "Tree Physiology",
title = "Hairy root culture as a valuable tool for allelopathic studies in apple",
number = "5",
volume = "39",
doi = "doi:10.1093/treephys/tpz006",
pages = "888-905"
}

Stanišić, M., Ćosić, T., Savić, J., Krstić Milošević, D., Mišić, D., Smigocki, A., Ninković, S.,& Banjac, N.. (2019). Hairy root culture as a valuable tool for allelopathic studies in apple. in Tree Physiology
Oxford: Oxford University Press., 39(5), 888-905.
https://doi.org/doi:10.1093/treephys/tpz006

Stanišić M, Ćosić T, Savić J, Krstić Milošević D, Mišić D, Smigocki A, Ninković S, Banjac N. Hairy root culture as a valuable tool for allelopathic studies in apple. in Tree Physiology. 2019;39(5):888-905.
doi:doi:10.1093/treephys/tpz006 .

Stanišić, Mariana, Ćosić, Tatjana, Savić, Jelena, Krstić Milošević, Dijana, Mišić, Danijela, Smigocki, Ann, Ninković, Slavica, Banjac, Nevena, "Hairy root culture as a valuable tool for allelopathic studies in apple" in Tree Physiology, 39, no. 5 (2019):888-905,
https://doi.org/doi:10.1093/treephys/tpz006 . .