TY  - JOUR
AU  - Stojičić, Dragana
AU  - Budimir, Snežana
AU  - Čokeša, Vlado
AU  - Uzelac, Branka
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6521
AB  - Pinus peuce (Macedonian pine) is considered a valuable ornamental tree that is frequently planted in parks and gardens, especially in Western Europe. This endemic pine is one of the most valuable conifer species in its native range, which currently consists of only two disjunct populations restricted to small mountainous areas of the Balkans and is listed as a near-threatened species. The reproduction of Macedonian pine by seed is limited, so in vitro propagation methods have emerged as a promising tool for large-scale propagation. The objective of this study was to develop an improved system for the micropropagation of P. peuce from juvenile plant material using a short-term liquid cytokinin pulse. For that, explants derived from 4-week-old seedlings were pulse-treated with different concentrations of N6-benzyladenine (BA) for 1 or 2 h to stimulate the induction of axillary buds. The highest axillary shoot formation was achieved with 222 µM BA pulse treatment, with an average number of ~six shoots per explant. Elongated shoots (≥10 mm) were detached from the explants and pulse-treated with 0.27 or 1.08 mM α-naphthaleneacetic acid (NAA) or 0.25 or 0.98 mM indole-3-butyric acid (IBA) for 1 or 2 h. IBA was more effective than NAA and led to a maximum rooting percentage (up to 40%) and the highest number of acclimatized plants (15–20%). Rooted plants were successfully transferred to ex vitro conditions.
PB  - Basel : MDPI
T2  - Horticulturae
T1  - Optimization of In Vitro Regeneration of Pinus peuce (Gris.)
IS  - 1
VL  - 10
DO  - 10.3390/horticulturae10010097
SP  - 97
ER  - 

@article{
author = "Stojičić, Dragana and Budimir, Snežana and Čokeša, Vlado and Uzelac, Branka",
year = "2024",
abstract = "Pinus peuce (Macedonian pine) is considered a valuable ornamental tree that is frequently planted in parks and gardens, especially in Western Europe. This endemic pine is one of the most valuable conifer species in its native range, which currently consists of only two disjunct populations restricted to small mountainous areas of the Balkans and is listed as a near-threatened species. The reproduction of Macedonian pine by seed is limited, so in vitro propagation methods have emerged as a promising tool for large-scale propagation. The objective of this study was to develop an improved system for the micropropagation of P. peuce from juvenile plant material using a short-term liquid cytokinin pulse. For that, explants derived from 4-week-old seedlings were pulse-treated with different concentrations of N6-benzyladenine (BA) for 1 or 2 h to stimulate the induction of axillary buds. The highest axillary shoot formation was achieved with 222 µM BA pulse treatment, with an average number of ~six shoots per explant. Elongated shoots (≥10 mm) were detached from the explants and pulse-treated with 0.27 or 1.08 mM α-naphthaleneacetic acid (NAA) or 0.25 or 0.98 mM indole-3-butyric acid (IBA) for 1 or 2 h. IBA was more effective than NAA and led to a maximum rooting percentage (up to 40%) and the highest number of acclimatized plants (15–20%). Rooted plants were successfully transferred to ex vitro conditions.",
publisher = "Basel : MDPI",
journal = "Horticulturae",
title = "Optimization of In Vitro Regeneration of Pinus peuce (Gris.)",
number = "1",
volume = "10",
doi = "10.3390/horticulturae10010097",
pages = "97"
}

Stojičić, D., Budimir, S., Čokeša, V.,& Uzelac, B.. (2024). Optimization of In Vitro Regeneration of Pinus peuce (Gris.). in Horticulturae
Basel : MDPI., 10(1), 97.
https://doi.org/10.3390/horticulturae10010097

Stojičić D, Budimir S, Čokeša V, Uzelac B. Optimization of In Vitro Regeneration of Pinus peuce (Gris.). in Horticulturae. 2024;10(1):97.
doi:10.3390/horticulturae10010097 .

Stojičić, Dragana, Budimir, Snežana, Čokeša, Vlado, Uzelac, Branka, "Optimization of In Vitro Regeneration of Pinus peuce (Gris.)" in Horticulturae, 10, no. 1 (2024):97,
https://doi.org/10.3390/horticulturae10010097 . .

TY  - JOUR
AU  - Stojičić, Dragana
AU  - Tošić, Svetlana
AU  - Stojanović, Gordana
AU  - Zlatković, Bojan
AU  - Jovanović, Snežana
AU  - Budimir, Snežana
AU  - Uzelac, Branka
PY  - 2022
UR  - https://www.mdpi.com/2223-7747/11/2/198
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8778064
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4768
AB  - Clinopodium pulegium (Rochel) Bräuchler (Lamiaceae) is an endangered species endemic to the Southern Carpathians. It is characterized by the production of high amounts of essential oils, which emit volatile organic compounds (VOCs) that have an essential role in biotic and abiotic stress responses and in plant-plant and plant-insect interactions. The present study was initiated to phytochemically examine the influence of different carbon sources in the nutrition medium on VOC emissions of micropropagated C. pulegium plants, using gas chromatography-mass spectrometry analysis of headspace VOCs. The volatile profiles were subjected to multivariate analysis with respect to the presence, concentration and type of carbon source in the nutrient medium. In addition, the effect of different carbohydrates on the density and size of the leaf glandular trichomes, the main structures involved in the emission of VOCs, was determined. A total of 19 VOCs, primarily belonging to mono- and sesquiterpenes previously described in plants, were tentatively identified. Six VOCs were produced at levels higher than 2% of the total VOC emission, dominated by pulegone, ß-pinene and menthone. Inclusion of the carbohydrates in the culture media affected the production of the main leaf trichome-associated volatile allelochemicals although the qualitative composition of the volatiles changed only slightly. Multivariate analysis showed that the concentration, rather than the carbohydrate type, influenced the VOC profile.
PB  - Basel: MDPI
T2  - Plants (Basel, Switzerland)
T1  - Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Bräuchler: Effect of Carbon Source.
IS  - 2
VL  - 11
DO  - 10.3390/plants11020198
SP  - 198
ER  - 

@article{
author = "Stojičić, Dragana and Tošić, Svetlana and Stojanović, Gordana and Zlatković, Bojan and Jovanović, Snežana and Budimir, Snežana and Uzelac, Branka",
year = "2022",
abstract = "Clinopodium pulegium (Rochel) Bräuchler (Lamiaceae) is an endangered species endemic to the Southern Carpathians. It is characterized by the production of high amounts of essential oils, which emit volatile organic compounds (VOCs) that have an essential role in biotic and abiotic stress responses and in plant-plant and plant-insect interactions. The present study was initiated to phytochemically examine the influence of different carbon sources in the nutrition medium on VOC emissions of micropropagated C. pulegium plants, using gas chromatography-mass spectrometry analysis of headspace VOCs. The volatile profiles were subjected to multivariate analysis with respect to the presence, concentration and type of carbon source in the nutrient medium. In addition, the effect of different carbohydrates on the density and size of the leaf glandular trichomes, the main structures involved in the emission of VOCs, was determined. A total of 19 VOCs, primarily belonging to mono- and sesquiterpenes previously described in plants, were tentatively identified. Six VOCs were produced at levels higher than 2% of the total VOC emission, dominated by pulegone, ß-pinene and menthone. Inclusion of the carbohydrates in the culture media affected the production of the main leaf trichome-associated volatile allelochemicals although the qualitative composition of the volatiles changed only slightly. Multivariate analysis showed that the concentration, rather than the carbohydrate type, influenced the VOC profile.",
publisher = "Basel: MDPI",
journal = "Plants (Basel, Switzerland)",
title = "Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Bräuchler: Effect of Carbon Source.",
number = "2",
volume = "11",
doi = "10.3390/plants11020198",
pages = "198"
}

Stojičić, D., Tošić, S., Stojanović, G., Zlatković, B., Jovanović, S., Budimir, S.,& Uzelac, B.. (2022). Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Bräuchler: Effect of Carbon Source.. in Plants (Basel, Switzerland)
Basel: MDPI., 11(2), 198.
https://doi.org/10.3390/plants11020198

Stojičić D, Tošić S, Stojanović G, Zlatković B, Jovanović S, Budimir S, Uzelac B. Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Bräuchler: Effect of Carbon Source.. in Plants (Basel, Switzerland). 2022;11(2):198.
doi:10.3390/plants11020198 .

Stojičić, Dragana, Tošić, Svetlana, Stojanović, Gordana, Zlatković, Bojan, Jovanović, Snežana, Budimir, Snežana, Uzelac, Branka, "Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Bräuchler: Effect of Carbon Source." in Plants (Basel, Switzerland), 11, no. 2 (2022):198,
https://doi.org/10.3390/plants11020198 . .

TY  - JOUR
AU  - Uzelac, Branka
AU  - Budimir, Snežana
AU  - Stojičić, Dragana
PY  - 2022
UR  - https://journal.pmf.ni.ac.rs/bionys/index.php/bionys/article/view/477
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5661
AB  - Many of the species belonging to the Lamiaceae family are considered aromatic
plants due to the presence of glandular trichomes, which have a distinct ability
to synthesize, secrete or store large amounts of specialized metabolites that
play a crucial role in mediating the plant–environment interactions. These
compounds often have marked bioactive properties, rendering a commercial
value to the plants that produce them. A number of biological effects have
been associated with the main monoterpenoids detected in investigated
Micromeria spp. and Clinopodium spp. essential oils. One alternative for the
production of these bioactive metabolites is in vitro plant tissue culture. The
present study was initiated to investigate the effects of in vitro culture on
the secretion of leaf glandular trichomes, the main structures involved in the
essential oil production. The glandular indumentum was studied by means of
light microscopy and scanning electron microscopy in an attempt to correlate
the phytochemical traits with the glandular trichome morphotypes of selected
Lamiaceae species.
PB  - Niš: University of Niš
T2  - Biologica Nyssana
T1  - Structure and chemistry of glandular trichomes of selected Micromeria and Clinopodium species (Lamiaceae): in vitro culture approach
IS  - 2
VL  - 13
DO  - 10.5281/zenodo.7476250
SP  - 191
EP  - 203
ER  - 

@article{
author = "Uzelac, Branka and Budimir, Snežana and Stojičić, Dragana",
year = "2022",
abstract = "Many of the species belonging to the Lamiaceae family are considered aromatic
plants due to the presence of glandular trichomes, which have a distinct ability
to synthesize, secrete or store large amounts of specialized metabolites that
play a crucial role in mediating the plant–environment interactions. These
compounds often have marked bioactive properties, rendering a commercial
value to the plants that produce them. A number of biological effects have
been associated with the main monoterpenoids detected in investigated
Micromeria spp. and Clinopodium spp. essential oils. One alternative for the
production of these bioactive metabolites is in vitro plant tissue culture. The
present study was initiated to investigate the effects of in vitro culture on
the secretion of leaf glandular trichomes, the main structures involved in the
essential oil production. The glandular indumentum was studied by means of
light microscopy and scanning electron microscopy in an attempt to correlate
the phytochemical traits with the glandular trichome morphotypes of selected
Lamiaceae species.",
publisher = "Niš: University of Niš",
journal = "Biologica Nyssana",
title = "Structure and chemistry of glandular trichomes of selected Micromeria and Clinopodium species (Lamiaceae): in vitro culture approach",
number = "2",
volume = "13",
doi = "10.5281/zenodo.7476250",
pages = "191-203"
}

Uzelac, B., Budimir, S.,& Stojičić, D.. (2022). Structure and chemistry of glandular trichomes of selected Micromeria and Clinopodium species (Lamiaceae): in vitro culture approach. in Biologica Nyssana
Niš: University of Niš., 13(2), 191-203.
https://doi.org/10.5281/zenodo.7476250

Uzelac B, Budimir S, Stojičić D. Structure and chemistry of glandular trichomes of selected Micromeria and Clinopodium species (Lamiaceae): in vitro culture approach. in Biologica Nyssana. 2022;13(2):191-203.
doi:10.5281/zenodo.7476250 .

Uzelac, Branka, Budimir, Snežana, Stojičić, Dragana, "Structure and chemistry of glandular trichomes of selected Micromeria and Clinopodium species (Lamiaceae): in vitro culture approach" in Biologica Nyssana, 13, no. 2 (2022):191-203,
https://doi.org/10.5281/zenodo.7476250 . .

TY  - CONF
AU  - Uzelac, Branka
AU  - Stojičić, Dragana
AU  - Budimir, Snežana
AU  - Tošić, Svetlana
AU  - Zlatković, Bojan
AU  - Blagojević, Saša
AU  - Manić, Branislav
AU  - Janjanin, Mirjana
AU  - Slavkovska, Violeta
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5664
AB  - Secondary metabolism in plant plays a major role in the survival of the plant in its ecosystem, mediating the interaction of the plant with its environment. Plant bioactive compounds are biosynthesized as a defensive strategy of plants in response to natural perturbations. A number of biological effects have been associated with the main monoterpenoids detected in investigated Micromeria spp. and Clinopodium spp. essential oils. One alternative for the production of these prospective biocontrol products is in vitro plant tissue culture. Our data suggest that the metabolic potential of in vitro shoot cultures of selected species can be manipulated by varying in vitro culture conditions.
PB  - Kragujevac: Agronomsku fakultet u Čačku, Univerzitet u Kragujevcu
C3  - Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia
T1  - Essential oils as potential biocontrol products against plant pathogens and weeds: in vitro culture approach
DO  - 10.46793/SBT27.345U
SP  - 345
EP  - 350
ER  - 

@conference{
author = "Uzelac, Branka and Stojičić, Dragana and Budimir, Snežana and Tošić, Svetlana and Zlatković, Bojan and Blagojević, Saša and Manić, Branislav and Janjanin, Mirjana and Slavkovska, Violeta",
year = "2022",
abstract = "Secondary metabolism in plant plays a major role in the survival of the plant in its ecosystem, mediating the interaction of the plant with its environment. Plant bioactive compounds are biosynthesized as a defensive strategy of plants in response to natural perturbations. A number of biological effects have been associated with the main monoterpenoids detected in investigated Micromeria spp. and Clinopodium spp. essential oils. One alternative for the production of these prospective biocontrol products is in vitro plant tissue culture. Our data suggest that the metabolic potential of in vitro shoot cultures of selected species can be manipulated by varying in vitro culture conditions.",
publisher = "Kragujevac: Agronomsku fakultet u Čačku, Univerzitet u Kragujevcu",
journal = "Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia",
title = "Essential oils as potential biocontrol products against plant pathogens and weeds: in vitro culture approach",
doi = "10.46793/SBT27.345U",
pages = "345-350"
}

Uzelac, B., Stojičić, D., Budimir, S., Tošić, S., Zlatković, B., Blagojević, S., Manić, B., Janjanin, M.,& Slavkovska, V.. (2022). Essential oils as potential biocontrol products against plant pathogens and weeds: in vitro culture approach. in Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia
Kragujevac: Agronomsku fakultet u Čačku, Univerzitet u Kragujevcu., 345-350.
https://doi.org/10.46793/SBT27.345U

Uzelac B, Stojičić D, Budimir S, Tošić S, Zlatković B, Blagojević S, Manić B, Janjanin M, Slavkovska V. Essential oils as potential biocontrol products against plant pathogens and weeds: in vitro culture approach. in Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia. 2022;:345-350.
doi:10.46793/SBT27.345U .

Uzelac, Branka, Stojičić, Dragana, Budimir, Snežana, Tošić, Svetlana, Zlatković, Bojan, Blagojević, Saša, Manić, Branislav, Janjanin, Mirjana, Slavkovska, Violeta, "Essential oils as potential biocontrol products against plant pathogens and weeds: in vitro culture approach" in Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia (2022):345-350,
https://doi.org/10.46793/SBT27.345U . .

TY  - CONF
AU  - Stojičić, Dragana
AU  - Tošić, Svetlana
AU  - Zlatković, Bojan
AU  - Blagojević, Saša
AU  - Manić, Branislav
AU  - Budimir, Snežana
AU  - Uzelac, Branka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5662
AB  - Clinopodium thymifolium (Scop.) Kuntze (syn. Micromeria thymifolia (Scop.) Fritsch) is a Balkan endemic species, traditionally used as a condiment and medicinal plant in the Mediterranean area. Clinopodium species were shown to produce considerable quantities (> 0.5%) of essential oils. The essential oil of wild-growing C. thymifolium possesses high antimicrobial activity. One alternative for the production of commercially important plant-derived metabolites, especially of rare plant genotypes, is in vitro plant tissue culture, given that the metabolic potential of in vitro shoot cultures can be manipulated by varying in vitro culture conditions. In view of the potential pharmacological and commercial value of C. thymifolium, the present study was initiated to propagate this aromatic plant species with the aim to increase the accumulation of biomass and the production of secondary metabolites. Shoots of wild-growing plants, dissected into one-node stem segments bearing two axillary buds, were used to establish in vitro cultures. Shoot multiplication was carried out on basal nutritional medium and on medium supplemented with various plant growth regulators at different concentrations. Nodal segments developed axillary shoots, and were successfully propagated on basal medium, whereas tested plant growth regulators differently affected their multiplication and biomass production.
PB  - Niš: Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
C3  - Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia
T1  - Micropropagation of Clinopodium thymifolium (Scop.) Kuntze (Lamiaceae)
SP  - 113
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5662
ER  - 

@conference{
editor = "Ranđelović, Vladimir, Stojanović-Radić, Zorica, Nikolić, Danijela, Jenačković Gocić, Dragana",
author = "Stojičić, Dragana and Tošić, Svetlana and Zlatković, Bojan and Blagojević, Saša and Manić, Branislav and Budimir, Snežana and Uzelac, Branka",
year = "2022",
abstract = "Clinopodium thymifolium (Scop.) Kuntze (syn. Micromeria thymifolia (Scop.) Fritsch) is a Balkan endemic species, traditionally used as a condiment and medicinal plant in the Mediterranean area. Clinopodium species were shown to produce considerable quantities (> 0.5%) of essential oils. The essential oil of wild-growing C. thymifolium possesses high antimicrobial activity. One alternative for the production of commercially important plant-derived metabolites, especially of rare plant genotypes, is in vitro plant tissue culture, given that the metabolic potential of in vitro shoot cultures can be manipulated by varying in vitro culture conditions. In view of the potential pharmacological and commercial value of C. thymifolium, the present study was initiated to propagate this aromatic plant species with the aim to increase the accumulation of biomass and the production of secondary metabolites. Shoots of wild-growing plants, dissected into one-node stem segments bearing two axillary buds, were used to establish in vitro cultures. Shoot multiplication was carried out on basal nutritional medium and on medium supplemented with various plant growth regulators at different concentrations. Nodal segments developed axillary shoots, and were successfully propagated on basal medium, whereas tested plant growth regulators differently affected their multiplication and biomass production.",
publisher = "Niš: Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš",
journal = "Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia",
title = "Micropropagation of Clinopodium thymifolium (Scop.) Kuntze (Lamiaceae)",
pages = "113",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5662"
}

Ranđelović, V., Stojanović-Radić, Z., Nikolić, D., Jenačković Gocić, D., Stojičić, D., Tošić, S., Zlatković, B., Blagojević, S., Manić, B., Budimir, S.,& Uzelac, B.. (2022). Micropropagation of Clinopodium thymifolium (Scop.) Kuntze (Lamiaceae). in Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia
Niš: Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 113.
https://hdl.handle.net/21.15107/rcub_ibiss_5662

Ranđelović V, Stojanović-Radić Z, Nikolić D, Jenačković Gocić D, Stojičić D, Tošić S, Zlatković B, Blagojević S, Manić B, Budimir S, Uzelac B. Micropropagation of Clinopodium thymifolium (Scop.) Kuntze (Lamiaceae). in Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia. 2022;:113.
https://hdl.handle.net/21.15107/rcub_ibiss_5662 .

Ranđelović, Vladimir, Stojanović-Radić, Zorica, Nikolić, Danijela, Jenačković Gocić, Dragana, Stojičić, Dragana, Tošić, Svetlana, Zlatković, Bojan, Blagojević, Saša, Manić, Branislav, Budimir, Snežana, Uzelac, Branka, "Micropropagation of Clinopodium thymifolium (Scop.) Kuntze (Lamiaceae)" in Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia (2022):113,
https://hdl.handle.net/21.15107/rcub_ibiss_5662 .

TY  - CONF
AU  - Uzelac, Branka
AU  - Budimir, Snežana
AU  - Stojičić, Dragana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5663
AB  - Many of the species belonging to the Lamiaceae family are considered aromatic plants due to the presence of glandular trichomes, which have a distinct ability to synthesize, secrete or store large amounts of specialized metabolites that play a crucial role in mediating the plant–environment interactions. Secondary metabolites are biosynthesized as a defensive strategy of plants in response to natural perturbations, thus enabling them to adapt to the environmental stresses typical of each individual ecological niche. These compounds often have marked bioactive properties, rendering a commercial value to the plants that produce them. A number of biological effects have been associated with the main monoterpenoids detected in investigated Micromeria spp. and Clinopodium spp. essential oils. One alternative for the production of these bioactive metabolites is in vitro plant tissue culture. The present study was initiated to investigate the effects of in vitro culture on the secretion of leaf glandular trichomes, the main structures involved in the essential oil production. The glandular indumentum was studied by means of light microscopy and scanning electron microscopy in an attempt to correlate the phytochemical traits with the glandular trichome morphotypes of selected Lamiaceae species.
PB  - Niš: Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
C3  - Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia
T1  - Insight into the structure and chemistry of glandular trichomes of selected Micromeria and closely related Clinopodium species (Lamiaceae): the in vitro culture approach
SP  - 120
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5663
ER  - 

@conference{
author = "Uzelac, Branka and Budimir, Snežana and Stojičić, Dragana",
year = "2022",
abstract = "Many of the species belonging to the Lamiaceae family are considered aromatic plants due to the presence of glandular trichomes, which have a distinct ability to synthesize, secrete or store large amounts of specialized metabolites that play a crucial role in mediating the plant–environment interactions. Secondary metabolites are biosynthesized as a defensive strategy of plants in response to natural perturbations, thus enabling them to adapt to the environmental stresses typical of each individual ecological niche. These compounds often have marked bioactive properties, rendering a commercial value to the plants that produce them. A number of biological effects have been associated with the main monoterpenoids detected in investigated Micromeria spp. and Clinopodium spp. essential oils. One alternative for the production of these bioactive metabolites is in vitro plant tissue culture. The present study was initiated to investigate the effects of in vitro culture on the secretion of leaf glandular trichomes, the main structures involved in the essential oil production. The glandular indumentum was studied by means of light microscopy and scanning electron microscopy in an attempt to correlate the phytochemical traits with the glandular trichome morphotypes of selected Lamiaceae species.",
publisher = "Niš: Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš",
journal = "Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia",
title = "Insight into the structure and chemistry of glandular trichomes of selected Micromeria and closely related Clinopodium species (Lamiaceae): the in vitro culture approach",
pages = "120",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5663"
}

Uzelac, B., Budimir, S.,& Stojičić, D.. (2022). Insight into the structure and chemistry of glandular trichomes of selected Micromeria and closely related Clinopodium species (Lamiaceae): the in vitro culture approach. in Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia
Niš: Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 120.
https://hdl.handle.net/21.15107/rcub_ibiss_5663

Uzelac B, Budimir S, Stojičić D. Insight into the structure and chemistry of glandular trichomes of selected Micromeria and closely related Clinopodium species (Lamiaceae): the in vitro culture approach. in Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia. 2022;:120.
https://hdl.handle.net/21.15107/rcub_ibiss_5663 .

Uzelac, Branka, Budimir, Snežana, Stojičić, Dragana, "Insight into the structure and chemistry of glandular trichomes of selected Micromeria and closely related Clinopodium species (Lamiaceae): the in vitro culture approach" in Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia (2022):120,
https://hdl.handle.net/21.15107/rcub_ibiss_5663 .

TY  - CHAP
AU  - Uzelac, Branka
AU  - Stojičić, Dragana
AU  - Budimir, Snežana
PY  - 2020
UR  - https://link.springer.com/referenceworkentry/10.1007/978-3-030-11253-0_1-1
UR  - http://link.springer.com/10.1007/978-3-030-11253-0_1-1
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3729
AB  - Glandular trichomes found on the surface of many higher plants contain specialized cells that produce and secrete copious amounts of particular secretory products. Leaf glandular trichomes of the non-model plant species Nicotiana tabacum represent a biologically active and stress-responsive tissue that contributes to plant defense response against biotic and abiotic stress and also influences leaf aroma and smoke flavor. Two morphologically different types of tobacco capitate trichomes, long- and short-stalked, with distinct functions, display ultrastructural features that are common to terpene-secreting glands, but only the secretory cells of the tall glandular trichomes are considered to be the site of biosynthesis of certain exudate compounds, including diterpenes and sucrose esters. Ultrastructural and histochemical characterization of tall glandular trichomes is described in an attempt to understand the contribution of these glands to the total secretion produced. Possible roles of distinct cellular compartments involved in the secretory process and secondary metabolite secretion under in vitro conditions are discussed.
PB  - Cham: Springer
T2  - Plant Cell and Tissue Differentiation and Secondary Metabolites
T1  - Glandular Trichomes on the Leaves of Nicotiana tabacum: Morphology, Developmental Ultrastructure, and Secondary Metabolites
DO  - 10.1007/978-3-030-11253-0_1-1
ER  - 

@inbook{
author = "Uzelac, Branka and Stojičić, Dragana and Budimir, Snežana",
year = "2020",
abstract = "Glandular trichomes found on the surface of many higher plants contain specialized cells that produce and secrete copious amounts of particular secretory products. Leaf glandular trichomes of the non-model plant species Nicotiana tabacum represent a biologically active and stress-responsive tissue that contributes to plant defense response against biotic and abiotic stress and also influences leaf aroma and smoke flavor. Two morphologically different types of tobacco capitate trichomes, long- and short-stalked, with distinct functions, display ultrastructural features that are common to terpene-secreting glands, but only the secretory cells of the tall glandular trichomes are considered to be the site of biosynthesis of certain exudate compounds, including diterpenes and sucrose esters. Ultrastructural and histochemical characterization of tall glandular trichomes is described in an attempt to understand the contribution of these glands to the total secretion produced. Possible roles of distinct cellular compartments involved in the secretory process and secondary metabolite secretion under in vitro conditions are discussed.",
publisher = "Cham: Springer",
journal = "Plant Cell and Tissue Differentiation and Secondary Metabolites",
booktitle = "Glandular Trichomes on the Leaves of Nicotiana tabacum: Morphology, Developmental Ultrastructure, and Secondary Metabolites",
doi = "10.1007/978-3-030-11253-0_1-1"
}

Uzelac, B., Stojičić, D.,& Budimir, S.. (2020). Glandular Trichomes on the Leaves of Nicotiana tabacum: Morphology, Developmental Ultrastructure, and Secondary Metabolites. in Plant Cell and Tissue Differentiation and Secondary Metabolites
Cham: Springer..
https://doi.org/10.1007/978-3-030-11253-0_1-1

Uzelac B, Stojičić D, Budimir S. Glandular Trichomes on the Leaves of Nicotiana tabacum: Morphology, Developmental Ultrastructure, and Secondary Metabolites. in Plant Cell and Tissue Differentiation and Secondary Metabolites. 2020;.
doi:10.1007/978-3-030-11253-0_1-1 .

Uzelac, Branka, Stojičić, Dragana, Budimir, Snežana, "Glandular Trichomes on the Leaves of Nicotiana tabacum: Morphology, Developmental Ultrastructure, and Secondary Metabolites" in Plant Cell and Tissue Differentiation and Secondary Metabolites (2020),
https://doi.org/10.1007/978-3-030-11253-0_1-1 . .

TY  - CONF
AU  - Janjanin, Mirjana
AU  - Tošić, Svetlana
AU  - Stojičić, Dragana
AU  - Zlatković, Bojan
AU  - Budimir, Snežana
AU  - Uzelac, Branka
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6719
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Proceedings: 14th Multinational Congress on Microscopy; 2019 Sep 15-20; Belgrade, Serbia
T1  - Micromorphological traits of Micromeria graeca (L.) Benth. ex Rchb. (Lamiaceae) leaf glandular trichomes of in vitro propagated plants
DO  - 978-86-80335-11-7
SP  - 154
EP  - 156
ER  - 

@conference{
author = "Janjanin, Mirjana and Tošić, Svetlana and Stojičić, Dragana and Zlatković, Bojan and Budimir, Snežana and Uzelac, Branka",
year = "2019",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Proceedings: 14th Multinational Congress on Microscopy; 2019 Sep 15-20; Belgrade, Serbia",
title = "Micromorphological traits of Micromeria graeca (L.) Benth. ex Rchb. (Lamiaceae) leaf glandular trichomes of in vitro propagated plants",
doi = "978-86-80335-11-7",
pages = "154-156"
}

Janjanin, M., Tošić, S., Stojičić, D., Zlatković, B., Budimir, S.,& Uzelac, B.. (2019). Micromorphological traits of Micromeria graeca (L.) Benth. ex Rchb. (Lamiaceae) leaf glandular trichomes of in vitro propagated plants. in Proceedings: 14th Multinational Congress on Microscopy; 2019 Sep 15-20; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 154-156.
https://doi.org/978-86-80335-11-7

Janjanin M, Tošić S, Stojičić D, Zlatković B, Budimir S, Uzelac B. Micromorphological traits of Micromeria graeca (L.) Benth. ex Rchb. (Lamiaceae) leaf glandular trichomes of in vitro propagated plants. in Proceedings: 14th Multinational Congress on Microscopy; 2019 Sep 15-20; Belgrade, Serbia. 2019;:154-156.
doi:978-86-80335-11-7 .

Janjanin, Mirjana, Tošić, Svetlana, Stojičić, Dragana, Zlatković, Bojan, Budimir, Snežana, Uzelac, Branka, "Micromorphological traits of Micromeria graeca (L.) Benth. ex Rchb. (Lamiaceae) leaf glandular trichomes of in vitro propagated plants" in Proceedings: 14th Multinational Congress on Microscopy; 2019 Sep 15-20; Belgrade, Serbia (2019):154-156,
https://doi.org/978-86-80335-11-7 . .

TY  - JOUR
AU  - Tošić, Svetlana
AU  - Stojičić, Dragana
AU  - Slavkovska, Violeta
AU  - Mihailov-Krstev, Tatjana
AU  - Zlatković, Bojan
AU  - Budimir, Snežana
AU  - Uzelac, Branka
PY  - 2019
UR  - http://link.springer.com/10.1007/s00425-018-03071-5
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3256
AB  - MAIN CONCLUSION In vitro culture conditions and kinetin induced quantitative modifications in the production of the major volatile constituents in Micromeria croatica plantlets. Antimicrobial activity of methanolic extracts obtained from micropropagated and wild-growing plants was evaluated. Micromeria spp. are aromatic plants, many of which were shown to exhibit various biological effects. The present study aimed to determine the content and the composition of the essential oil of in vitro-cultured Micromeria croatica (Pers.) Schott and to evaluate the in vitro antimicrobial activity of its methanolic extract, in order to compare its phytochemical profile and biological activity with wild-growing plants. Shoots regenerated on MS medium without plant growth regulators (PGRs) or supplemented with kinetin were used for phytochemical analysis. Essential oils from both native plant material and in vitro-cultivated M. croatica plants, with a total of 44 identified constituents, were dominated by oxygenated monoterpenes. Borneol was the main component detected in wild-growing plants (25.28%) and micropropagated plants grown on PGR-free medium (20.30%). Kinetin treatment led to increased oil yield and favored the production of oxygenated monoterpenes, dominated by geranial (33.53%) and cis-p-mentha-1(7),8-dien-ol (23.69%). The percentage of total sesquiterpenoids in micropropagated plant material was considerably lower than in wild-growing plants. In vitro culture conditions and PGRs affected the production of essential oils, inducing quantitative modifications in the production of the major volatile constituents in M. croatica plantlets. The antimicrobial activity of M. croatica methanolic extracts was investigated using the broth microdilution method. Extracts obtained from in vitro cultures generally exhibited greater antibacterial potential, compared to wild-growing plants. Among six bacterial strains tested, Bacillus cereus and Staphylococcus aureus were the most sensitive microorganisms. The present study provided evidence that in vitro culture conditions might favorably affect the antimicrobial activity of M. croatica methanolic extracts.
PB  - Springer Berlin Heidelberg
T2  - Planta
T1  - Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae).
DO  - 10.1007/s00425-018-03071-5
ER  - 

@article{
author = "Tošić, Svetlana and Stojičić, Dragana and Slavkovska, Violeta and Mihailov-Krstev, Tatjana and Zlatković, Bojan and Budimir, Snežana and Uzelac, Branka",
year = "2019",
abstract = "MAIN CONCLUSION In vitro culture conditions and kinetin induced quantitative modifications in the production of the major volatile constituents in Micromeria croatica plantlets. Antimicrobial activity of methanolic extracts obtained from micropropagated and wild-growing plants was evaluated. Micromeria spp. are aromatic plants, many of which were shown to exhibit various biological effects. The present study aimed to determine the content and the composition of the essential oil of in vitro-cultured Micromeria croatica (Pers.) Schott and to evaluate the in vitro antimicrobial activity of its methanolic extract, in order to compare its phytochemical profile and biological activity with wild-growing plants. Shoots regenerated on MS medium without plant growth regulators (PGRs) or supplemented with kinetin were used for phytochemical analysis. Essential oils from both native plant material and in vitro-cultivated M. croatica plants, with a total of 44 identified constituents, were dominated by oxygenated monoterpenes. Borneol was the main component detected in wild-growing plants (25.28%) and micropropagated plants grown on PGR-free medium (20.30%). Kinetin treatment led to increased oil yield and favored the production of oxygenated monoterpenes, dominated by geranial (33.53%) and cis-p-mentha-1(7),8-dien-ol (23.69%). The percentage of total sesquiterpenoids in micropropagated plant material was considerably lower than in wild-growing plants. In vitro culture conditions and PGRs affected the production of essential oils, inducing quantitative modifications in the production of the major volatile constituents in M. croatica plantlets. The antimicrobial activity of M. croatica methanolic extracts was investigated using the broth microdilution method. Extracts obtained from in vitro cultures generally exhibited greater antibacterial potential, compared to wild-growing plants. Among six bacterial strains tested, Bacillus cereus and Staphylococcus aureus were the most sensitive microorganisms. The present study provided evidence that in vitro culture conditions might favorably affect the antimicrobial activity of M. croatica methanolic extracts.",
publisher = "Springer Berlin Heidelberg",
journal = "Planta",
title = "Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae).",
doi = "10.1007/s00425-018-03071-5"
}

Tošić, S., Stojičić, D., Slavkovska, V., Mihailov-Krstev, T., Zlatković, B., Budimir, S.,& Uzelac, B.. (2019). Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae).. in Planta
Springer Berlin Heidelberg..
https://doi.org/10.1007/s00425-018-03071-5

Tošić S, Stojičić D, Slavkovska V, Mihailov-Krstev T, Zlatković B, Budimir S, Uzelac B. Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae).. in Planta. 2019;.
doi:10.1007/s00425-018-03071-5 .

Tošić, Svetlana, Stojičić, Dragana, Slavkovska, Violeta, Mihailov-Krstev, Tatjana, Zlatković, Bojan, Budimir, Snežana, Uzelac, Branka, "Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae)." in Planta (2019),
https://doi.org/10.1007/s00425-018-03071-5 . .

TY  - CHAP
AU  - Stojičić, Dragana
AU  - Uzelac, Branka
AU  - Budimir, Snežana
PY  - 2018
UR  - http://link.springer.com/10.1007/978-3-319-89483-6_4
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3270
AB  - Bosnian pine (Pinus heldreichii Christ.) is a Tertiary relict subendemic to the mountains of Balkan Peninsula and a small area in southern Italy.
PB  - Springer
T2  - Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants
T1  - Bosnian Pine Pinus heldreichii Christ.
DO  - 10.1007/978-3-319-89483-6_4
SP  - 49
EP  - 62
ER  - 

@inbook{
author = "Stojičić, Dragana and Uzelac, Branka and Budimir, Snežana",
year = "2018",
abstract = "Bosnian pine (Pinus heldreichii Christ.) is a Tertiary relict subendemic to the mountains of Balkan Peninsula and a small area in southern Italy.",
publisher = "Springer",
journal = "Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants",
booktitle = "Bosnian Pine Pinus heldreichii Christ.",
doi = "10.1007/978-3-319-89483-6_4",
pages = "49-62"
}

Stojičić, D., Uzelac, B.,& Budimir, S.. (2018). Bosnian Pine Pinus heldreichii Christ.. in Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants
Springer., 49-62.
https://doi.org/10.1007/978-3-319-89483-6_4

Stojičić D, Uzelac B, Budimir S. Bosnian Pine Pinus heldreichii Christ.. in Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants. 2018;:49-62.
doi:10.1007/978-3-319-89483-6_4 .

Stojičić, Dragana, Uzelac, Branka, Budimir, Snežana, "Bosnian Pine Pinus heldreichii Christ." in Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants (2018):49-62,
https://doi.org/10.1007/978-3-319-89483-6_4 . .

TY  - CONF
AU  - Tošić, Svetlana
AU  - Stojičić, Dragana
AU  - Slavkovska, Violeta
AU  - Zlatković, Bojan
AU  - Budimir, Snežana
AU  - Uzelac, Branka
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6735
AB  - Morphology and structure of Micromeria croatica glandular trichomes in relation to the secretory process were characterized using scanning electron microscopy (SEM) and light microscopy, and their secretion was histochemically analyzed. SEM and histochemical analysis revealed that leaf indumentum of in vitro grown M. croatica consisted of non-glandular and glandular trichomes (Figure 1a). The main types of glandular trichomes observed in M. croatica leaves were peltate and capitate (Figure 1b-d). Peltate trichomes consisted of a broad basal cell embedded in the epidermis, a single-celled stalk, and a large round multicellular head (Figure 1e, h). Two types of capitate trichomes, differing in size and structure, could be distinguished. Type I capitate trichomes were positioned at an angle to the leaf surface and composed of one basal epidermal cell, short cutinized stalk and unicellular ellipsoidal head (Figure 1f). Type II capitate trichomes comprised of one basal cell, unicellular stalk and one apical secretory cell (Figure 1g). Apical cell of mature type II capitate trichomes had well developed round subcuticular space (Figure 1d). Plant extracts secreted from glandular trichomes are a valuable source of biologically active compounds. Phytochemical analysis identified up to 37 compounds in the essential oils (EOs) of wild-growing and micropropagated plants, and revealed differences in both content and the composition of the EOs obtained from different samples. EOs from both native plant material and in vitro cultivated plants showed very high percentages of total monoterpenoids, dominated by oxygenated monoterpenes. 
Borneol was the main essential oil component detected in wild-growing plants (25.28%) and micropropagated plants grown on plant growth regulator-free medium (20.30%). Micropropagated plants cultivated in the presence of 0.3 µM kinetin had the highest yield of the EO, with geranial (33.53%) and cis-p-mentha-1(7),8-dien-ol (23.69%) being the main EO components. The percentage of total sesquiterpenoids in micropropagated plant material was considerably lower than in wild-growing plants. Sesquiterpene hydrocarbons accounted for the majority of detected sesquiterpenoids and were dominated by alpha-cadinene and beta-vetivenene. Some of the volatiles were found only in plant material obtained by micropropagation but not in wild-growing plants. Differences in both content and the composition of the essential oils obtained from different samples indicated that in vitro culture conditions and plant growth regulators significantly influence the essential oils properties.
PB  - Zagreb: Ruđer Bošković Institute and Croatian Microscopy Society
C3  - Book of Abstract: 13th Multinational Congress on Microscopy; 2017 Sep 24-29; Rovinj, Croatia
T1  - Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria croatica (Pers.) Schott (Lamiaceae)
SP  - 302
EP  - 303
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6735
ER  - 

@conference{
author = "Tošić, Svetlana and Stojičić, Dragana and Slavkovska, Violeta and Zlatković, Bojan and Budimir, Snežana and Uzelac, Branka",
year = "2017",
abstract = "Morphology and structure of Micromeria croatica glandular trichomes in relation to the secretory process were characterized using scanning electron microscopy (SEM) and light microscopy, and their secretion was histochemically analyzed. SEM and histochemical analysis revealed that leaf indumentum of in vitro grown M. croatica consisted of non-glandular and glandular trichomes (Figure 1a). The main types of glandular trichomes observed in M. croatica leaves were peltate and capitate (Figure 1b-d). Peltate trichomes consisted of a broad basal cell embedded in the epidermis, a single-celled stalk, and a large round multicellular head (Figure 1e, h). Two types of capitate trichomes, differing in size and structure, could be distinguished. Type I capitate trichomes were positioned at an angle to the leaf surface and composed of one basal epidermal cell, short cutinized stalk and unicellular ellipsoidal head (Figure 1f). Type II capitate trichomes comprised of one basal cell, unicellular stalk and one apical secretory cell (Figure 1g). Apical cell of mature type II capitate trichomes had well developed round subcuticular space (Figure 1d). Plant extracts secreted from glandular trichomes are a valuable source of biologically active compounds. Phytochemical analysis identified up to 37 compounds in the essential oils (EOs) of wild-growing and micropropagated plants, and revealed differences in both content and the composition of the EOs obtained from different samples. EOs from both native plant material and in vitro cultivated plants showed very high percentages of total monoterpenoids, dominated by oxygenated monoterpenes. 
Borneol was the main essential oil component detected in wild-growing plants (25.28%) and micropropagated plants grown on plant growth regulator-free medium (20.30%). Micropropagated plants cultivated in the presence of 0.3 µM kinetin had the highest yield of the EO, with geranial (33.53%) and cis-p-mentha-1(7),8-dien-ol (23.69%) being the main EO components. The percentage of total sesquiterpenoids in micropropagated plant material was considerably lower than in wild-growing plants. Sesquiterpene hydrocarbons accounted for the majority of detected sesquiterpenoids and were dominated by alpha-cadinene and beta-vetivenene. Some of the volatiles were found only in plant material obtained by micropropagation but not in wild-growing plants. Differences in both content and the composition of the essential oils obtained from different samples indicated that in vitro culture conditions and plant growth regulators significantly influence the essential oils properties.",
publisher = "Zagreb: Ruđer Bošković Institute and Croatian Microscopy Society",
journal = "Book of Abstract: 13th Multinational Congress on Microscopy; 2017 Sep 24-29; Rovinj, Croatia",
title = "Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria croatica (Pers.) Schott (Lamiaceae)",
pages = "302-303",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6735"
}

Tošić, S., Stojičić, D., Slavkovska, V., Zlatković, B., Budimir, S.,& Uzelac, B.. (2017). Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria croatica (Pers.) Schott (Lamiaceae). in Book of Abstract: 13th Multinational Congress on Microscopy; 2017 Sep 24-29; Rovinj, Croatia
Zagreb: Ruđer Bošković Institute and Croatian Microscopy Society., 302-303.
https://hdl.handle.net/21.15107/rcub_ibiss_6735

Tošić S, Stojičić D, Slavkovska V, Zlatković B, Budimir S, Uzelac B. Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria croatica (Pers.) Schott (Lamiaceae). in Book of Abstract: 13th Multinational Congress on Microscopy; 2017 Sep 24-29; Rovinj, Croatia. 2017;:302-303.
https://hdl.handle.net/21.15107/rcub_ibiss_6735 .

Tošić, Svetlana, Stojičić, Dragana, Slavkovska, Violeta, Zlatković, Bojan, Budimir, Snežana, Uzelac, Branka, "Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria croatica (Pers.) Schott (Lamiaceae)" in Book of Abstract: 13th Multinational Congress on Microscopy; 2017 Sep 24-29; Rovinj, Croatia (2017):302-303,
https://hdl.handle.net/21.15107/rcub_ibiss_6735 .

TY  - JOUR
AU  - Uzelac, Branka
AU  - Janošević, Dušica
AU  - Stojičić, Dragana
AU  - Budimir, Snežana
PY  - 2017
UR  - http://doi.wiley.com/10.1002/jemt.22864
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2681
AB  - The anatomy and ultrastructure of the short glandular trichomes occurring on young expanding leaves of Nicotiana tabacum were investigated using light and transmission electron microscopy. The objective of the present research was to characterize the cellular changes that occur during morphogenesis of short glandular trichomes, from initiation to senescence. Ultrastructural analysis of their secretory cells revealed characteristics common to gland cells: numerous mitochondria in highly organized cytoplasm, large nuclei, and an elaborate network of endoplasmic reticulum. Initial changes in nuclear and plastidial organization were observed at a more advanced secretory stage, marking the onset of senescence. During trichome senescence, gradual reduction of the cytoplasm density occurred along with structural changes of the plastids and the tonoplast. As a result of inward blebbing of the cytoplasm into the vacuole, membrane bound vesicular structures appeared in the vacuolar space. At the late secretory stage, marked by an increase in vacuolation and extraplasmic space, degenerative changes included further fragmentation of the cytoplasm and deterioration of the tonoplast. Multimembrane myelin bodies observed in the vacuolar space were indicative of membrane digestion although plasma membrane did not appear massively degraded.
T2  - Microscopy Research and Technique
T1  - Morphogenesis and developmental ultrastructure of Nicotiana tabacum short glandular trichomes
DO  - 10.1002/jemt.22864
ER  - 

@article{
author = "Uzelac, Branka and Janošević, Dušica and Stojičić, Dragana and Budimir, Snežana",
year = "2017",
abstract = "The anatomy and ultrastructure of the short glandular trichomes occurring on young expanding leaves of Nicotiana tabacum were investigated using light and transmission electron microscopy. The objective of the present research was to characterize the cellular changes that occur during morphogenesis of short glandular trichomes, from initiation to senescence. Ultrastructural analysis of their secretory cells revealed characteristics common to gland cells: numerous mitochondria in highly organized cytoplasm, large nuclei, and an elaborate network of endoplasmic reticulum. Initial changes in nuclear and plastidial organization were observed at a more advanced secretory stage, marking the onset of senescence. During trichome senescence, gradual reduction of the cytoplasm density occurred along with structural changes of the plastids and the tonoplast. As a result of inward blebbing of the cytoplasm into the vacuole, membrane bound vesicular structures appeared in the vacuolar space. At the late secretory stage, marked by an increase in vacuolation and extraplasmic space, degenerative changes included further fragmentation of the cytoplasm and deterioration of the tonoplast. Multimembrane myelin bodies observed in the vacuolar space were indicative of membrane digestion although plasma membrane did not appear massively degraded.",
journal = "Microscopy Research and Technique",
title = "Morphogenesis and developmental ultrastructure of Nicotiana tabacum short glandular trichomes",
doi = "10.1002/jemt.22864"
}

Uzelac, B., Janošević, D., Stojičić, D.,& Budimir, S.. (2017). Morphogenesis and developmental ultrastructure of Nicotiana tabacum short glandular trichomes. in Microscopy Research and Technique.
https://doi.org/10.1002/jemt.22864

Uzelac B, Janošević D, Stojičić D, Budimir S. Morphogenesis and developmental ultrastructure of Nicotiana tabacum short glandular trichomes. in Microscopy Research and Technique. 2017;.
doi:10.1002/jemt.22864 .

Uzelac, Branka, Janošević, Dušica, Stojičić, Dragana, Budimir, Snežana, "Morphogenesis and developmental ultrastructure of Nicotiana tabacum short glandular trichomes" in Microscopy Research and Technique (2017),
https://doi.org/10.1002/jemt.22864 . .

TY  - JOUR
AU  - Stojičić, Dragana
AU  - Tošić, Svetlana
AU  - Slavkovska, Violeta
AU  - Zlatković, Bojan
AU  - Budimir, Snežana
AU  - Janošević, Dušica
AU  - Uzelac, Branka
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6200
AB  - The content and chemical composition of Micromeria
pulegium (Rochel) Benth. essential oils were studied in
native plant material at vegetative stage and in micropropagated
plants, obtained from nodal segments cultured
on solid MS medium supplemented with N6–benzyladenine
(BA) or kinetin at different concentrations, alone or in
combination with indole-3-acetic acid. Shoot proliferation
was achieved in all treatments, but the highest biomass
production was obtained after treatment with 10 μM BA.
Phytochemical analysis identified up to 21 compounds in
the essential oils of wild-growing and in vitro cultivated
plants, both showing very high percentages of total
monoterpenoids dominated by oxygenated monoterpenes
of the menthane type. Pulegone and menthone were the
main essential oil components detected in both wildgrowing
plants (60.07 and 26.85 %, respectively) and
micropropagated plants grown on either plant growth regulator-
free medium (44.57 and 29.14 %, respectively) or
BA-supplemented medium (50.77 and 14.45 %, respectively).
The percentage of total sesquiterpenoids increased
in vitro, particularly owing to sesquiterpene hydrocarbons
that were not found in wild-growing plants. Differences in
both content and the composition of the essential oils
obtained from different samples indicated that in vitro
culture conditions and plant growth regulators significantly
influence the essential oils properties. In addition, the
morphology and structure of M. pulegium glandular trichomes
in relation to the secretory process were characterized
for the first time using SEM and light microscopy,
and their secretion was histochemically analyzed.
PB  - Berlin Heidelberg : Springer-Verlag
T2  - Planta
T1  - Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae)
IS  - 2
VL  - 244
DO  - 10.1007/s00425-016-2513-7
SP  - 393
EP  - 404
ER  - 

@article{
author = "Stojičić, Dragana and Tošić, Svetlana and Slavkovska, Violeta and Zlatković, Bojan and Budimir, Snežana and Janošević, Dušica and Uzelac, Branka",
year = "2016",
abstract = "The content and chemical composition of Micromeria
pulegium (Rochel) Benth. essential oils were studied in
native plant material at vegetative stage and in micropropagated
plants, obtained from nodal segments cultured
on solid MS medium supplemented with N6–benzyladenine
(BA) or kinetin at different concentrations, alone or in
combination with indole-3-acetic acid. Shoot proliferation
was achieved in all treatments, but the highest biomass
production was obtained after treatment with 10 μM BA.
Phytochemical analysis identified up to 21 compounds in
the essential oils of wild-growing and in vitro cultivated
plants, both showing very high percentages of total
monoterpenoids dominated by oxygenated monoterpenes
of the menthane type. Pulegone and menthone were the
main essential oil components detected in both wildgrowing
plants (60.07 and 26.85 %, respectively) and
micropropagated plants grown on either plant growth regulator-
free medium (44.57 and 29.14 %, respectively) or
BA-supplemented medium (50.77 and 14.45 %, respectively).
The percentage of total sesquiterpenoids increased
in vitro, particularly owing to sesquiterpene hydrocarbons
that were not found in wild-growing plants. Differences in
both content and the composition of the essential oils
obtained from different samples indicated that in vitro
culture conditions and plant growth regulators significantly
influence the essential oils properties. In addition, the
morphology and structure of M. pulegium glandular trichomes
in relation to the secretory process were characterized
for the first time using SEM and light microscopy,
and their secretion was histochemically analyzed.",
publisher = "Berlin Heidelberg : Springer-Verlag",
journal = "Planta",
title = "Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae)",
number = "2",
volume = "244",
doi = "10.1007/s00425-016-2513-7",
pages = "393-404"
}

Stojičić, D., Tošić, S., Slavkovska, V., Zlatković, B., Budimir, S., Janošević, D.,& Uzelac, B.. (2016). Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae). in Planta
Berlin Heidelberg : Springer-Verlag., 244(2), 393-404.
https://doi.org/10.1007/s00425-016-2513-7

Stojičić D, Tošić S, Slavkovska V, Zlatković B, Budimir S, Janošević D, Uzelac B. Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae). in Planta. 2016;244(2):393-404.
doi:10.1007/s00425-016-2513-7 .

Stojičić, Dragana, Tošić, Svetlana, Slavkovska, Violeta, Zlatković, Bojan, Budimir, Snežana, Janošević, Dušica, Uzelac, Branka, "Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae)" in Planta, 244, no. 2 (2016):393-404,
https://doi.org/10.1007/s00425-016-2513-7 . .

TY  - JOUR
AU  - Uzelac, Branka
AU  - Janošević, Dušica
AU  - Stojičić, Dragana
AU  - Budimir, Snežana
PY  - 2015
UR  - https://botanicaserbica.bio.bg.ac.rs/arhiva/pdf/2015_39_2_632_full.pdf
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4063
AB  - The tall glandular trichomes occurring on young expanding leaves of Nicotiana tabacum grown in
vitro were investigated using light and electron microscopy. Previous studies reported that the tall
glandular trichomes of tobacco produce large quantities of various compounds including diterpenes.
To explore the cellular structures required and putatively involved in diterpene biosynthesis and
secretion, the present study focused on the development of the tall glandular trichomes, and on
histochemical and ultrastructural analysis of their secretory cells under in vitro conditions. Electron
microscopy confirmed earlier light microscopy observations of the glandular nature of the tall leaf
trichomes. Their head cells, which secrete resinous material, exhibited characteristics common
to gland cells: a dense cytoplasm, numerous mitochondria and little vacuolation. They contained
structurally well-developed chloroplasts and an elaborate network of endoplasmic reticulum, as
well as electron dense inclusions in chloroplasts and in small vacuoles. The dominance of smooth
endoplasmic reticulum suggests its involvement in diterpene biosynthesis. Histochemical tests were
positive for both lipophilic and hydrophilic secretions, which included terpenoids (essential oils
and resins) and acidic/neutral lipids, as well as phenolic compounds (tannins), alkaloids, mucilage/
pectin and polysaccharides.
PB  - Belgrade: Institute of Botany and Botanical Garden "Jevremovac"
T2  - Botanica Serbica
T1  - In vitro morphogenesis and secretion of secondary metabolites of Nicotiana tabacum tall glandular trichomes
IS  - 2
VL  - 39
SP  - 103
EP  - 110
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4063
ER  - 

@article{
author = "Uzelac, Branka and Janošević, Dušica and Stojičić, Dragana and Budimir, Snežana",
year = "2015",
abstract = "The tall glandular trichomes occurring on young expanding leaves of Nicotiana tabacum grown in
vitro were investigated using light and electron microscopy. Previous studies reported that the tall
glandular trichomes of tobacco produce large quantities of various compounds including diterpenes.
To explore the cellular structures required and putatively involved in diterpene biosynthesis and
secretion, the present study focused on the development of the tall glandular trichomes, and on
histochemical and ultrastructural analysis of their secretory cells under in vitro conditions. Electron
microscopy confirmed earlier light microscopy observations of the glandular nature of the tall leaf
trichomes. Their head cells, which secrete resinous material, exhibited characteristics common
to gland cells: a dense cytoplasm, numerous mitochondria and little vacuolation. They contained
structurally well-developed chloroplasts and an elaborate network of endoplasmic reticulum, as
well as electron dense inclusions in chloroplasts and in small vacuoles. The dominance of smooth
endoplasmic reticulum suggests its involvement in diterpene biosynthesis. Histochemical tests were
positive for both lipophilic and hydrophilic secretions, which included terpenoids (essential oils
and resins) and acidic/neutral lipids, as well as phenolic compounds (tannins), alkaloids, mucilage/
pectin and polysaccharides.",
publisher = "Belgrade: Institute of Botany and Botanical Garden "Jevremovac"",
journal = "Botanica Serbica",
title = "In vitro morphogenesis and secretion of secondary metabolites of Nicotiana tabacum tall glandular trichomes",
number = "2",
volume = "39",
pages = "103-110",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4063"
}

Uzelac, B., Janošević, D., Stojičić, D.,& Budimir, S.. (2015). In vitro morphogenesis and secretion of secondary metabolites of Nicotiana tabacum tall glandular trichomes. in Botanica Serbica
Belgrade: Institute of Botany and Botanical Garden "Jevremovac"., 39(2), 103-110.
https://hdl.handle.net/21.15107/rcub_ibiss_4063

Uzelac B, Janošević D, Stojičić D, Budimir S. In vitro morphogenesis and secretion of secondary metabolites of Nicotiana tabacum tall glandular trichomes. in Botanica Serbica. 2015;39(2):103-110.
https://hdl.handle.net/21.15107/rcub_ibiss_4063 .

Uzelac, Branka, Janošević, Dušica, Stojičić, Dragana, Budimir, Snežana, "In vitro morphogenesis and secretion of secondary metabolites of Nicotiana tabacum tall glandular trichomes" in Botanica Serbica, 39, no. 2 (2015):103-110,
https://hdl.handle.net/21.15107/rcub_ibiss_4063 .

TY  - JOUR
AU  - Uzelac, Branka
AU  - Janošević, Dušica
AU  - Stojičić, Dragana
AU  - Budimir, Snežana
PY  - 2012
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/308
AB  - Cytokinins are involved in plant cell proliferation leading to plant growth and morphogenesis. The size, activity and maintenance of the shoot apical meristem (SAM) are defined by a balanced rate of mitotic cell divisions and functional cell differentiation that are controlled by cytokinins. In order to investigate the effect of exogenous cytokinin on SAM, morpho-anatomical changes in the shoot apices of tobacco treated with benzyladenine (BA) were compared to those of untreated control plants.
T2  - Archives of Biological Sciences
T1  - Effect of cytokinins on shoot apical meristem in Nicotiana tabacum
IS  - 2
VL  - 64
SP  - 511
EP  - 516
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_308
ER  - 

@article{
author = "Uzelac, Branka and Janošević, Dušica and Stojičić, Dragana and Budimir, Snežana",
year = "2012, 2012",
abstract = "Cytokinins are involved in plant cell proliferation leading to plant growth and morphogenesis. The size, activity and maintenance of the shoot apical meristem (SAM) are defined by a balanced rate of mitotic cell divisions and functional cell differentiation that are controlled by cytokinins. In order to investigate the effect of exogenous cytokinin on SAM, morpho-anatomical changes in the shoot apices of tobacco treated with benzyladenine (BA) were compared to those of untreated control plants.",
journal = "Archives of Biological Sciences",
title = "Effect of cytokinins on shoot apical meristem in Nicotiana tabacum",
number = "2",
volume = "64",
pages = "511-516",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_308"
}

Uzelac, B., Janošević, D., Stojičić, D.,& Budimir, S.. (2012). Effect of cytokinins on shoot apical meristem in Nicotiana tabacum. in Archives of Biological Sciences, 64(2), 511-516.
https://hdl.handle.net/21.15107/rcub_ibiss_308

Uzelac B, Janošević D, Stojičić D, Budimir S. Effect of cytokinins on shoot apical meristem in Nicotiana tabacum. in Archives of Biological Sciences. 2012;64(2):511-516.
https://hdl.handle.net/21.15107/rcub_ibiss_308 .

Uzelac, Branka, Janošević, Dušica, Stojičić, Dragana, Budimir, Snežana, "Effect of cytokinins on shoot apical meristem in Nicotiana tabacum" in Archives of Biological Sciences, 64, no. 2 (2012):511-516,
https://hdl.handle.net/21.15107/rcub_ibiss_308 .

TY  - JOUR
AU  - Stojičić, Dragana
AU  - Janošević, Dušica
AU  - Uzelac, Branka
AU  - Čokeša, V.
AU  - Budimir, Snežana
PY  - 2012
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/307
AB  - This study reports a protocol for the germination and early seedling growth of Pinus peuce Gris. using zygotic embryo culture. In order to overcome seed dormancy and optimize organogenesis, the effect of nutritional, plant growth regulatory and physical factors on in vitro germination and growth of isolated mature zygotic embryos of P. peuce were investigated.
T2  - Archives of Biological Sciences
T1  - In vitro zygotic embryo culture of Pinus peuce Gris.: Optimization of culture conditions affecting germination and early seedling growth
IS  - 2
VL  - 64
SP  - 503
EP  - 509
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_307
ER  - 

@article{
author = "Stojičić, Dragana and Janošević, Dušica and Uzelac, Branka and Čokeša, V. and Budimir, Snežana",
year = "2012, 2012",
abstract = "This study reports a protocol for the germination and early seedling growth of Pinus peuce Gris. using zygotic embryo culture. In order to overcome seed dormancy and optimize organogenesis, the effect of nutritional, plant growth regulatory and physical factors on in vitro germination and growth of isolated mature zygotic embryos of P. peuce were investigated.",
journal = "Archives of Biological Sciences",
title = "In vitro zygotic embryo culture of Pinus peuce Gris.: Optimization of culture conditions affecting germination and early seedling growth",
number = "2",
volume = "64",
pages = "503-509",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_307"
}

Stojičić, D., Janošević, D., Uzelac, B., Čokeša, V.,& Budimir, S.. (2012). In vitro zygotic embryo culture of Pinus peuce Gris.: Optimization of culture conditions affecting germination and early seedling growth. in Archives of Biological Sciences, 64(2), 503-509.
https://hdl.handle.net/21.15107/rcub_ibiss_307

Stojičić D, Janošević D, Uzelac B, Čokeša V, Budimir S. In vitro zygotic embryo culture of Pinus peuce Gris.: Optimization of culture conditions affecting germination and early seedling growth. in Archives of Biological Sciences. 2012;64(2):503-509.
https://hdl.handle.net/21.15107/rcub_ibiss_307 .

Stojičić, Dragana, Janošević, Dušica, Uzelac, Branka, Čokeša, V., Budimir, Snežana, "In vitro zygotic embryo culture of Pinus peuce Gris.: Optimization of culture conditions affecting germination and early seedling growth" in Archives of Biological Sciences, 64, no. 2 (2012):503-509,
https://hdl.handle.net/21.15107/rcub_ibiss_307 .

TY  - JOUR
AU  - Janošević, Dušica
AU  - Stojičić, Dragana
AU  - Budimir, Snežana
PY  - 2008
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/520
AB  - The potential of somatic embryogenesis induction was examined by in vitro growing of isolated ovules of Pinus heldreichii (Christ.) on nutrient media. The induction of embryogenic tissue was stimulated by Gresshoff and Doy (GD) medium with nitrogen compounds reduced to one half and with 0.5 mg/l BA and 2.0 mg/l 2.4-D. Still, the most important factor in this process was the development stage of embryos in the ovule. An induction frequency of 10% was obtained on the ovules in which embryos were in the pre-cotyledonary stage. When the dominant embryo in the ovule was in the cotyledonary stage, the induction frequency was lower or absent. Proliferation of embryogenic tissue was achieved on the media with reduced concentration of growth regulators, and the initial stages of embryo maturation were obtained on the media with ABA and 5% sucrose.
AB  - Izolovane ovule Pinus heldreichii (Christ.) gajene su in vitro na hranljivim podlogama radi ispitivanja mogućnosti indukcije somatske embriogeneze. Hranljiva podloga Gresshoff and Doy (GD) sa azotnim jedinjenjima, smanjenim na polovinu i BA 0.5 mg/L i 2.4-D 2.0 mg/L, stimulisala je indukciju embriogenog tkiva. Ipak, najvažniji faktor u ovom procesu bio je stadijum razvića embriona u ovuli. Frekvencija indukcije od 10% dobijena je na ovulama u kojima su embrioni bili u prekotiledonarnom stadijumu razvića. Kada je dominantni embrion u ovuli bio u kotiledonarnom stadijumu razvića, frekvencija indukcije bila je manja ili je nije ni bilo. Proliferacija embriogenog tkiva dobijena je na podlogama sa smanjenom koncentracijom regulatora rastenja, a početni stadijumi sazrevanja embriona na podlogama sa ABA i 5% saharoze.
T2  - Šumarstvo
T1  - Uticaj stadijuma razvića megagametofita Pinus heldreichii na indukciju embriogenog tkiva
T1  - Effect of development stage of Pinus heldreichii megagametophytes on the induction of embryogenic tissue
IS  - 1-2
VL  - 60
SP  - 93
EP  - 101
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_520
ER  - 

@article{
author = "Janošević, Dušica and Stojičić, Dragana and Budimir, Snežana",
year = "2008, 2008",
abstract = "The potential of somatic embryogenesis induction was examined by in vitro growing of isolated ovules of Pinus heldreichii (Christ.) on nutrient media. The induction of embryogenic tissue was stimulated by Gresshoff and Doy (GD) medium with nitrogen compounds reduced to one half and with 0.5 mg/l BA and 2.0 mg/l 2.4-D. Still, the most important factor in this process was the development stage of embryos in the ovule. An induction frequency of 10% was obtained on the ovules in which embryos were in the pre-cotyledonary stage. When the dominant embryo in the ovule was in the cotyledonary stage, the induction frequency was lower or absent. Proliferation of embryogenic tissue was achieved on the media with reduced concentration of growth regulators, and the initial stages of embryo maturation were obtained on the media with ABA and 5% sucrose., Izolovane ovule Pinus heldreichii (Christ.) gajene su in vitro na hranljivim podlogama radi ispitivanja mogućnosti indukcije somatske embriogeneze. Hranljiva podloga Gresshoff and Doy (GD) sa azotnim jedinjenjima, smanjenim na polovinu i BA 0.5 mg/L i 2.4-D 2.0 mg/L, stimulisala je indukciju embriogenog tkiva. Ipak, najvažniji faktor u ovom procesu bio je stadijum razvića embriona u ovuli. Frekvencija indukcije od 10% dobijena je na ovulama u kojima su embrioni bili u prekotiledonarnom stadijumu razvića. Kada je dominantni embrion u ovuli bio u kotiledonarnom stadijumu razvića, frekvencija indukcije bila je manja ili je nije ni bilo. Proliferacija embriogenog tkiva dobijena je na podlogama sa smanjenom koncentracijom regulatora rastenja, a početni stadijumi sazrevanja embriona na podlogama sa ABA i 5% saharoze.",
journal = "Šumarstvo",
title = "Uticaj stadijuma razvića megagametofita Pinus heldreichii na indukciju embriogenog tkiva, Effect of development stage of Pinus heldreichii megagametophytes on the induction of embryogenic tissue",
number = "1-2",
volume = "60",
pages = "93-101",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_520"
}

Janošević, D., Stojičić, D.,& Budimir, S.. (2008). Uticaj stadijuma razvića megagametofita Pinus heldreichii na indukciju embriogenog tkiva. in Šumarstvo, 60(1-2), 93-101.
https://hdl.handle.net/21.15107/rcub_ibiss_520

Janošević D, Stojičić D, Budimir S. Uticaj stadijuma razvića megagametofita Pinus heldreichii na indukciju embriogenog tkiva. in Šumarstvo. 2008;60(1-2):93-101.
https://hdl.handle.net/21.15107/rcub_ibiss_520 .

Janošević, Dušica, Stojičić, Dragana, Budimir, Snežana, "Uticaj stadijuma razvića megagametofita Pinus heldreichii na indukciju embriogenog tkiva" in Šumarstvo, 60, no. 1-2 (2008):93-101,
https://hdl.handle.net/21.15107/rcub_ibiss_520 .

TY  - JOUR
AU  - Stojičić, Dragana
AU  - Janošević, Dušica
AU  - Uzelac, Branka
AU  - Budimir, Snežana
PY  - 2008
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/214
AB  - The effects of nutritional, hormonal, and physical factors on the germination and growth of isolated mature zygotic embryos of Pinus heldreichii were studied under in vitro conditions. The optimum medium for embryo germination and adequate conversion into seedlings was basal Gresshoff and Doy (1GD) medium. All tested carbohydrates at the applied concentrations stimulated embryo growth, but only 3% sucrose increased embryo germination compared to the control, while maltose had an inhibitory effect. Among the applied plant growth regulators, only gibberelic acid (GA3) had a few benefits on the development of isolated embryos into plantlets, while the others had a negative effect. Embryos cultured on an agar-solidified medium grew better and the germination percentage was higher than in a liquid medium. Light treatment did not affect embryo germination, but generally stimulated seedling growth.
AB  - U kulturi in vitro ispitivan je efekat nutritivnih, hormonalnih i fizičkih faktora na klijanje i rastenje izolovanih zigotskih embriona munike (Pinus heldrechii). Većina izolovanih embriona munike klijala je već tokom prve nedelje nakon prenošenja na hranljivu podlogu. Mada embrioni mogu da klijaju i na podlozi bez soli, vitamina i ugljenih hidrata, najveći procenat klijanja i najbolje morfološke karakteristike klijanaca postignute su na bazalnoj Gresshoff & Doy podlozi (1GD) sa 3% saharozom. Od ispitivanih regulatora rastenja samo je giberelna kiselina (GA3) pozitivno delovala na razviće embriona, dok su auksini i citokinini inhibirali njihovo dalje razviće. U odnosu na embrione koji su rasli u tečnoj podlozi, embrioni sa čvrste agarne podloge, klijali su u većem procentu i dalje se pravilno razvijali. Mada generalno stimulišu rastenje klijanaca, svetlosni tretmani nisu imali efekat na klijanje embriona. Dobijeni rezultati pokazuju da se in vitro sistem može koristiti za brzu i sinhronu produkciju zdravih klijanaca elitnih genotipova koji se odlikuju niskom produkcijom semena.
T2  - Archives of Biological Sciences
T1  - Klijanje i rastenje izolovanih embriona Pinus heldreichii u kulturi in vitro
T1  - Factors influencing germination and growth of isolated embryos of Pinus heldreichii
IS  - 4
VL  - 60
SP  - 673
EP  - 679
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_214
ER  - 

@article{
author = "Stojičić, Dragana and Janošević, Dušica and Uzelac, Branka and Budimir, Snežana",
year = "2008, 2008",
abstract = "The effects of nutritional, hormonal, and physical factors on the germination and growth of isolated mature zygotic embryos of Pinus heldreichii were studied under in vitro conditions. The optimum medium for embryo germination and adequate conversion into seedlings was basal Gresshoff and Doy (1GD) medium. All tested carbohydrates at the applied concentrations stimulated embryo growth, but only 3% sucrose increased embryo germination compared to the control, while maltose had an inhibitory effect. Among the applied plant growth regulators, only gibberelic acid (GA3) had a few benefits on the development of isolated embryos into plantlets, while the others had a negative effect. Embryos cultured on an agar-solidified medium grew better and the germination percentage was higher than in a liquid medium. Light treatment did not affect embryo germination, but generally stimulated seedling growth., U kulturi in vitro ispitivan je efekat nutritivnih, hormonalnih i fizičkih faktora na klijanje i rastenje izolovanih zigotskih embriona munike (Pinus heldrechii). Većina izolovanih embriona munike klijala je već tokom prve nedelje nakon prenošenja na hranljivu podlogu. Mada embrioni mogu da klijaju i na podlozi bez soli, vitamina i ugljenih hidrata, najveći procenat klijanja i najbolje morfološke karakteristike klijanaca postignute su na bazalnoj Gresshoff & Doy podlozi (1GD) sa 3% saharozom. Od ispitivanih regulatora rastenja samo je giberelna kiselina (GA3) pozitivno delovala na razviće embriona, dok su auksini i citokinini inhibirali njihovo dalje razviće. U odnosu na embrione koji su rasli u tečnoj podlozi, embrioni sa čvrste agarne podloge, klijali su u većem procentu i dalje se pravilno razvijali. Mada generalno stimulišu rastenje klijanaca, svetlosni tretmani nisu imali efekat na klijanje embriona. Dobijeni rezultati pokazuju da se in vitro sistem može koristiti za brzu i sinhronu produkciju zdravih klijanaca elitnih genotipova koji se odlikuju niskom produkcijom semena.",
journal = "Archives of Biological Sciences",
title = "Klijanje i rastenje izolovanih embriona Pinus heldreichii u kulturi in vitro, Factors influencing germination and growth of isolated embryos of Pinus heldreichii",
number = "4",
volume = "60",
pages = "673-679",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_214"
}

Stojičić, D., Janošević, D., Uzelac, B.,& Budimir, S.. (2008). Klijanje i rastenje izolovanih embriona Pinus heldreichii u kulturi in vitro. in Archives of Biological Sciences, 60(4), 673-679.
https://hdl.handle.net/21.15107/rcub_ibiss_214

Stojičić D, Janošević D, Uzelac B, Budimir S. Klijanje i rastenje izolovanih embriona Pinus heldreichii u kulturi in vitro. in Archives of Biological Sciences. 2008;60(4):673-679.
https://hdl.handle.net/21.15107/rcub_ibiss_214 .

Stojičić, Dragana, Janošević, Dušica, Uzelac, Branka, Budimir, Snežana, "Klijanje i rastenje izolovanih embriona Pinus heldreichii u kulturi in vitro" in Archives of Biological Sciences, 60, no. 4 (2008):673-679,
https://hdl.handle.net/21.15107/rcub_ibiss_214 .

TY  - JOUR
AU  - Stojičić, Dragana
AU  - Uzelac, Branka
AU  - Janošević, Dušica
AU  - Ćulafić, Ljubinka
AU  - Budimir, Snežana
PY  - 2007
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/159
AB  - The potential for somatic embryogenesis in zygotic embryo and megagametophyte cultures of Pinus heldreichii was examined. Somatic embryogenesis was initiated from megagametophytes containing immature zygotic embryos at early stages of development. An induction frequency of up to 6.7% was obtained on Gresshoff and Doy medium in the presence of 2 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 mg/l benzyladenine (BA). Formation and further proliferation of embryogenic tissue were achieved upon transfer of explants to a medium with reduced levels of growth regulators. Somatic embryos are being cultured for further development. .
AB  - U kulturi izolovanih zigotskih embriona i ovula munike (Pinus heldreichii) ispitivan je efekat hranljive podloge i regulatora rastenja na indukciju somatske embriogeneze. Somatska embriogeneza je indukovana u kulturi ovula koje su sadržavale embrione na ranom stupnju razvića. Najviša frekvencija indukcije od 6.7% postignuta je kada su ovule 5 dana gajene na Gresshoff i Doy (GD) hranljivoj podlozi u prisustvu 2,4-D 2 mg/l i BA 0.5 mg/l, a zatim prenete na GD podlogu u kojoj je koncentracija regulatora rastenja bila 5 puta niža. U cilju dugotrajnog održavanja u kulturi, embriogeno tkivo je izolovano i gajeno na GD podlozi sa 2,4-D 0.2 mg/l i BA 0.05 mg/l. Mikroskopskom analizom je utvrđeno da se tkivo sastoji od brojnih embriona na ranim stupnjevima razvića. Za klonalnu propagaciju ove značajne endemoreliktne četinarske vrste u kulturi in vitro, neophodna su dalja istraživanja sa ciljem povećanja frekvencije indukcije somatske embriogeneze, kao i frekvencije sazrevanja embriona. .
T2  - Archives of Biological Sciences
T1  - Indukcija somatske embriogeneze kod Pinus heldreichii u kulturi
T1  - Induction of somatic embryogenesis in Pinus heldreichii culture
IS  - 3
VL  - 59
SP  - 199
EP  - 202
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_159
ER  - 

@article{
author = "Stojičić, Dragana and Uzelac, Branka and Janošević, Dušica and Ćulafić, Ljubinka and Budimir, Snežana",
year = "2007, 2007",
abstract = "The potential for somatic embryogenesis in zygotic embryo and megagametophyte cultures of Pinus heldreichii was examined. Somatic embryogenesis was initiated from megagametophytes containing immature zygotic embryos at early stages of development. An induction frequency of up to 6.7% was obtained on Gresshoff and Doy medium in the presence of 2 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 mg/l benzyladenine (BA). Formation and further proliferation of embryogenic tissue were achieved upon transfer of explants to a medium with reduced levels of growth regulators. Somatic embryos are being cultured for further development. ., U kulturi izolovanih zigotskih embriona i ovula munike (Pinus heldreichii) ispitivan je efekat hranljive podloge i regulatora rastenja na indukciju somatske embriogeneze. Somatska embriogeneza je indukovana u kulturi ovula koje su sadržavale embrione na ranom stupnju razvića. Najviša frekvencija indukcije od 6.7% postignuta je kada su ovule 5 dana gajene na Gresshoff i Doy (GD) hranljivoj podlozi u prisustvu 2,4-D 2 mg/l i BA 0.5 mg/l, a zatim prenete na GD podlogu u kojoj je koncentracija regulatora rastenja bila 5 puta niža. U cilju dugotrajnog održavanja u kulturi, embriogeno tkivo je izolovano i gajeno na GD podlozi sa 2,4-D 0.2 mg/l i BA 0.05 mg/l. Mikroskopskom analizom je utvrđeno da se tkivo sastoji od brojnih embriona na ranim stupnjevima razvića. Za klonalnu propagaciju ove značajne endemoreliktne četinarske vrste u kulturi in vitro, neophodna su dalja istraživanja sa ciljem povećanja frekvencije indukcije somatske embriogeneze, kao i frekvencije sazrevanja embriona. .",
journal = "Archives of Biological Sciences",
title = "Indukcija somatske embriogeneze kod Pinus heldreichii u kulturi, Induction of somatic embryogenesis in Pinus heldreichii culture",
number = "3",
volume = "59",
pages = "199-202",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_159"
}

Stojičić, D., Uzelac, B., Janošević, D., Ćulafić, L.,& Budimir, S.. (2007). Indukcija somatske embriogeneze kod Pinus heldreichii u kulturi. in Archives of Biological Sciences, 59(3), 199-202.
https://hdl.handle.net/21.15107/rcub_ibiss_159

Stojičić D, Uzelac B, Janošević D, Ćulafić L, Budimir S. Indukcija somatske embriogeneze kod Pinus heldreichii u kulturi. in Archives of Biological Sciences. 2007;59(3):199-202.
https://hdl.handle.net/21.15107/rcub_ibiss_159 .

Stojičić, Dragana, Uzelac, Branka, Janošević, Dušica, Ćulafić, Ljubinka, Budimir, Snežana, "Indukcija somatske embriogeneze kod Pinus heldreichii u kulturi" in Archives of Biological Sciences, 59, no. 3 (2007):199-202,
https://hdl.handle.net/21.15107/rcub_ibiss_159 .