TY  - JOUR
AU  - Erić, Katarina
AU  - Savić Veselinović, Marija
AU  - Patenković, Aleksandra
AU  - Davidović, Slobodan
AU  - Erić, Pavle
AU  - Stamenković-Radak, Marina
AU  - Tanasković, Marija
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5865
AB  - Drosophila subobscura is considered a good model species for investigation of a population’s ability to adapt and cope with climate changes. Decade long research has shown that inversion frequencies change in response to environmental factors indicating their role in adaptation to novel environments. The mechanisms behind organisms’ responses to temperature are complex, involving changes in physiology, behavior, gene expression and regulation. On the other hand, a population’s ability to respond to suboptimal conditions depends on standing genetic variation and population history. In order to elucidate the role of local adaptation in population response to the changing temperature, we investigated the response to temperature in D. subobscura individuals originating from two different altitudes by combining traditional cytogenetic techniques with assessing the levels of Hsp70 protein expression. Inversion polymorphism was assessed in the flies sampled from natural populations and in flies reared in laboratory conditions at three different temperatures after five and sixteen generations and Hsp70 protein expression profile in 12th generation flies at the basal level and after heat shock induction. Our results indicate that local adaptation and population history influence population response to the changing temperature.
PB  - Basel: MDPI
T2  - Life
T1  - Population History Shapes Responses to Different Temperature Regimes in Drosophila subobscura
IS  - 6
VL  - 13
DO  - 10.3390/life13061333
SP  - 1333
ER  - 

@article{
author = "Erić, Katarina and Savić Veselinović, Marija and Patenković, Aleksandra and Davidović, Slobodan and Erić, Pavle and Stamenković-Radak, Marina and Tanasković, Marija",
year = "2023",
abstract = "Drosophila subobscura is considered a good model species for investigation of a population’s ability to adapt and cope with climate changes. Decade long research has shown that inversion frequencies change in response to environmental factors indicating their role in adaptation to novel environments. The mechanisms behind organisms’ responses to temperature are complex, involving changes in physiology, behavior, gene expression and regulation. On the other hand, a population’s ability to respond to suboptimal conditions depends on standing genetic variation and population history. In order to elucidate the role of local adaptation in population response to the changing temperature, we investigated the response to temperature in D. subobscura individuals originating from two different altitudes by combining traditional cytogenetic techniques with assessing the levels of Hsp70 protein expression. Inversion polymorphism was assessed in the flies sampled from natural populations and in flies reared in laboratory conditions at three different temperatures after five and sixteen generations and Hsp70 protein expression profile in 12th generation flies at the basal level and after heat shock induction. Our results indicate that local adaptation and population history influence population response to the changing temperature.",
publisher = "Basel: MDPI",
journal = "Life",
title = "Population History Shapes Responses to Different Temperature Regimes in Drosophila subobscura",
number = "6",
volume = "13",
doi = "10.3390/life13061333",
pages = "1333"
}

Erić, K., Savić Veselinović, M., Patenković, A., Davidović, S., Erić, P., Stamenković-Radak, M.,& Tanasković, M.. (2023). Population History Shapes Responses to Different Temperature Regimes in Drosophila subobscura. in Life
Basel: MDPI., 13(6), 1333.
https://doi.org/10.3390/life13061333

Erić K, Savić Veselinović M, Patenković A, Davidović S, Erić P, Stamenković-Radak M, Tanasković M. Population History Shapes Responses to Different Temperature Regimes in Drosophila subobscura. in Life. 2023;13(6):1333.
doi:10.3390/life13061333 .

Erić, Katarina, Savić Veselinović, Marija, Patenković, Aleksandra, Davidović, Slobodan, Erić, Pavle, Stamenković-Radak, Marina, Tanasković, Marija, "Population History Shapes Responses to Different Temperature Regimes in Drosophila subobscura" in Life, 13, no. 6 (2023):1333,
https://doi.org/10.3390/life13061333 . .

TY  - JOUR
AU  - Davidović, Slobodan
AU  - Marinković, Saša
AU  - Kukobat, Mila
AU  - Mihajlović, Milica
AU  - Tanasić, Vanja
AU  - Hribšek, Irena
AU  - Tanasković, Marija
AU  - Stamenković-Radak, Marina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4751
AB  - Once a widespread and common species across the region of southeast Europe, the Griffon vulture is now confined to small and isolated populations across the Balkan Peninsula. The population from Serbia with 290 couples represents its biggest and most viable population that can serve as an important reservoir of genetic diversity from which the birds can be used for the region’s reintroduction or recolonization programs. To estimate the level of genetic diversity, the mitochondrial Cytb gene from 58 unrelated birds sampled during the marking in the nests was sequenced and compared to the homologous Griffon vulture sequences available in publicly accessible online databases. Phylogeographic analysis based on Cytb sequences showed that the most frequent haplotype is found in all Griffon vulture populations and that each population possesses private haplotypes. Our data suggest that the Griffon vulture population from Serbia should be used as a source population for restocking and reintroduction programs in the region. The observed genetic differentiation between the populations from the Iberian and Balkan Peninsulas suggest that the introduction of foreign birds from remote populations should be avoided and that birds from indigenous or neighboring populations, if available, should be used instead.
PB  - Basel: MDPI
T2  - Life
T1  - Genetic Diversity Analysis of Mitochondrial Cytb Gene, Phylogeny and Phylogeography of Protected Griffon Vulture (Gyps fulvus) from Serbia
IS  - 2
VL  - 12
DO  - 10.3390/life12020164
SP  - 164
ER  - 

@article{
author = "Davidović, Slobodan and Marinković, Saša and Kukobat, Mila and Mihajlović, Milica and Tanasić, Vanja and Hribšek, Irena and Tanasković, Marija and Stamenković-Radak, Marina",
year = "2022",
abstract = "Once a widespread and common species across the region of southeast Europe, the Griffon vulture is now confined to small and isolated populations across the Balkan Peninsula. The population from Serbia with 290 couples represents its biggest and most viable population that can serve as an important reservoir of genetic diversity from which the birds can be used for the region’s reintroduction or recolonization programs. To estimate the level of genetic diversity, the mitochondrial Cytb gene from 58 unrelated birds sampled during the marking in the nests was sequenced and compared to the homologous Griffon vulture sequences available in publicly accessible online databases. Phylogeographic analysis based on Cytb sequences showed that the most frequent haplotype is found in all Griffon vulture populations and that each population possesses private haplotypes. Our data suggest that the Griffon vulture population from Serbia should be used as a source population for restocking and reintroduction programs in the region. The observed genetic differentiation between the populations from the Iberian and Balkan Peninsulas suggest that the introduction of foreign birds from remote populations should be avoided and that birds from indigenous or neighboring populations, if available, should be used instead.",
publisher = "Basel: MDPI",
journal = "Life",
title = "Genetic Diversity Analysis of Mitochondrial Cytb Gene, Phylogeny and Phylogeography of Protected Griffon Vulture (Gyps fulvus) from Serbia",
number = "2",
volume = "12",
doi = "10.3390/life12020164",
pages = "164"
}

Davidović, S., Marinković, S., Kukobat, M., Mihajlović, M., Tanasić, V., Hribšek, I., Tanasković, M.,& Stamenković-Radak, M.. (2022). Genetic Diversity Analysis of Mitochondrial Cytb Gene, Phylogeny and Phylogeography of Protected Griffon Vulture (Gyps fulvus) from Serbia. in Life
Basel: MDPI., 12(2), 164.
https://doi.org/10.3390/life12020164

Davidović S, Marinković S, Kukobat M, Mihajlović M, Tanasić V, Hribšek I, Tanasković M, Stamenković-Radak M. Genetic Diversity Analysis of Mitochondrial Cytb Gene, Phylogeny and Phylogeography of Protected Griffon Vulture (Gyps fulvus) from Serbia. in Life. 2022;12(2):164.
doi:10.3390/life12020164 .

Davidović, Slobodan, Marinković, Saša, Kukobat, Mila, Mihajlović, Milica, Tanasić, Vanja, Hribšek, Irena, Tanasković, Marija, Stamenković-Radak, Marina, "Genetic Diversity Analysis of Mitochondrial Cytb Gene, Phylogeny and Phylogeography of Protected Griffon Vulture (Gyps fulvus) from Serbia" in Life, 12, no. 2 (2022):164,
https://doi.org/10.3390/life12020164 . .

TY  - JOUR
AU  - Davidović, Slobodan
AU  - Marinković, Saša
AU  - Hribšek, Irena
AU  - Patenković, Aleksandra
AU  - Stamenković-Radak, Marina
AU  - Tanasković, Marija
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5304
AB  - Background: Once a widespread species across the region of Southeast Europe, the
Griffon vulture is now confined to small and isolated populations across the Balkan
Peninsula. The population from Serbia represents its biggest and most viable
population that can serve as an important reservoir of genetic diversity from which
the birds can be used for the region’s reintroduction programmes. The available
genetic data for this valuable population are scarce and as a protected species that
belongs to the highly endangered vulture group, it needs to be well described so that it
can be properly managed and used as a restocking population. Considering the
serious recent bottleneck event that the Griffon vulture population from Serbia
experienced we estimated the overall relatedness among the birds from this
population. Sex ratio, another important parameter that shows the vitality and
strength of the population was evaluated as well.
Methods: During the annual monitoring that was performed in the period from
2013–2021, we collected blood samples from individual birds that were marked in the
nests. In total, 169 samples were collected and each was used for molecular sexing
while 58 presumably unrelated birds from different nests were used for inbreeding
and relatedness analyses. The relatedness was estimated using both biparentally (10
microsatellite loci) and uniparentally (Cytb and D-loop I of mitochondrial DNA)
inherited markers.
Results: The level of inbreeding was relatively high and on average it was 8.3% while
the mean number of relatives for each bird was close to three. The sex ratio was close
to 1:1 and for the analysed period of 9 years, it didn’t demonstrate a statistically
significant deviation from the expected ratio of 1:1, suggesting that this is a stable and
healthy population. Our data suggest that, even though a relatively high level of
inbreeding can be detected among the individual birds, the Griffon vulture
population from Serbia can be used as a source population for restocking and
reintroduction programmes in the region. These data combined with previously
observed genetic differentiation between the populations from the Iberian and
Balkan Peninsulas suggest that the introduction of foreign birds should be avoided
and that local birds should be used instead.
PB  - PeerJ
T2  - PeerJ
T1  - Sex ratio and relatedness in the Griffon vulture (Gyps fulvus) population of Serbia
VL  - 10
DO  - 10.7717/peerj.14477
SP  - e14477
ER  - 

@article{
author = "Davidović, Slobodan and Marinković, Saša and Hribšek, Irena and Patenković, Aleksandra and Stamenković-Radak, Marina and Tanasković, Marija",
year = "2022",
abstract = "Background: Once a widespread species across the region of Southeast Europe, the
Griffon vulture is now confined to small and isolated populations across the Balkan
Peninsula. The population from Serbia represents its biggest and most viable
population that can serve as an important reservoir of genetic diversity from which
the birds can be used for the region’s reintroduction programmes. The available
genetic data for this valuable population are scarce and as a protected species that
belongs to the highly endangered vulture group, it needs to be well described so that it
can be properly managed and used as a restocking population. Considering the
serious recent bottleneck event that the Griffon vulture population from Serbia
experienced we estimated the overall relatedness among the birds from this
population. Sex ratio, another important parameter that shows the vitality and
strength of the population was evaluated as well.
Methods: During the annual monitoring that was performed in the period from
2013–2021, we collected blood samples from individual birds that were marked in the
nests. In total, 169 samples were collected and each was used for molecular sexing
while 58 presumably unrelated birds from different nests were used for inbreeding
and relatedness analyses. The relatedness was estimated using both biparentally (10
microsatellite loci) and uniparentally (Cytb and D-loop I of mitochondrial DNA)
inherited markers.
Results: The level of inbreeding was relatively high and on average it was 8.3% while
the mean number of relatives for each bird was close to three. The sex ratio was close
to 1:1 and for the analysed period of 9 years, it didn’t demonstrate a statistically
significant deviation from the expected ratio of 1:1, suggesting that this is a stable and
healthy population. Our data suggest that, even though a relatively high level of
inbreeding can be detected among the individual birds, the Griffon vulture
population from Serbia can be used as a source population for restocking and
reintroduction programmes in the region. These data combined with previously
observed genetic differentiation between the populations from the Iberian and
Balkan Peninsulas suggest that the introduction of foreign birds should be avoided
and that local birds should be used instead.",
publisher = "PeerJ",
journal = "PeerJ",
title = "Sex ratio and relatedness in the Griffon vulture (Gyps fulvus) population of Serbia",
volume = "10",
doi = "10.7717/peerj.14477",
pages = "e14477"
}

Davidović, S., Marinković, S., Hribšek, I., Patenković, A., Stamenković-Radak, M.,& Tanasković, M.. (2022). Sex ratio and relatedness in the Griffon vulture (Gyps fulvus) population of Serbia. in PeerJ
PeerJ., 10, e14477.
https://doi.org/10.7717/peerj.14477

Davidović S, Marinković S, Hribšek I, Patenković A, Stamenković-Radak M, Tanasković M. Sex ratio and relatedness in the Griffon vulture (Gyps fulvus) population of Serbia. in PeerJ. 2022;10:e14477.
doi:10.7717/peerj.14477 .

Davidović, Slobodan, Marinković, Saša, Hribšek, Irena, Patenković, Aleksandra, Stamenković-Radak, Marina, Tanasković, Marija, "Sex ratio and relatedness in the Griffon vulture (Gyps fulvus) population of Serbia" in PeerJ, 10 (2022):e14477,
https://doi.org/10.7717/peerj.14477 . .

TY  - CONF
AU  - Tanasković, Marija
AU  - Marinković, Saša
AU  - Hribšek, Irena
AU  - Patenković, Aleksandra
AU  - Anđus, Stefan
AU  - Stamenković-Radak, Marina
AU  - Davidović, Slobodan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5040
AB  - Beloglavi sup, Gyps fulvus, nekada široko rasprostranjena vrsta širom Jugoistočne Evrope danas je ograničen na male i izolovane populacije od kojih je na Balkanu najbrojnija na teritoriji Srbije. Genetička varijabilnost populacije beloglavog supa u Srbiji procenjena je na osnovu 58 ptica, na nivou mitohondrijske DNK analizom sekvence CytB i na nivou jedarne DNK analizom 10 mikrosatelitskih lokusa, dok je molekularnim markerima određen pol izleglih jedinki u periodu od 2013-2022 godine. Bez obzira na skorašnji drastičan pad brojnosti, nivo genetičke varijabilnosti procenjen na osnovu izabranih markera sličan je ostalim analiziranim populacijama u svetu. Analiza mikrosatelitskih lokusa je pokazala da populacija beloglavog supa iz Srbije poseduje najveći broj privatnih haplotipova, ali i nešto veći nivo inbridinga (8,3%). Detektovana su tri CytB haplotipa (jedan koji je prisutan u svim populacijama i dva karakteristična samo za populaciju iz Srbije). Na osnovu svih analiziranih markera, možemo reći da populacija supova u Srbiji poseduje genetičke osobenosti koje je jasno odvajaju od svih ostalih do sada analiziranih populacija kao i da je pokazan jednak odnos polova dodatni indikator stabilnosti i dobrog „zdravlja“ ove populacije. Populaciono genetičke analize pokazuju da je populacija beloglavog supa sa teritorije Srbije optimalni izbor za buduće programe reintrodukcije u regionu, uz napomenu da bi trebalo izbegavati reintrodukciju jedinki poreklom iz drugih geografskih područja.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Genetička varijabilnost populacije beloglavog supa na teritoriji Srbije – perspektiva za buduće programe reintrodukcije u regionu
SP  - 89
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5040
ER  - 

@conference{
author = "Tanasković, Marija and Marinković, Saša and Hribšek, Irena and Patenković, Aleksandra and Anđus, Stefan and Stamenković-Radak, Marina and Davidović, Slobodan",
year = "2022",
abstract = "Beloglavi sup, Gyps fulvus, nekada široko rasprostranjena vrsta širom Jugoistočne Evrope danas je ograničen na male i izolovane populacije od kojih je na Balkanu najbrojnija na teritoriji Srbije. Genetička varijabilnost populacije beloglavog supa u Srbiji procenjena je na osnovu 58 ptica, na nivou mitohondrijske DNK analizom sekvence CytB i na nivou jedarne DNK analizom 10 mikrosatelitskih lokusa, dok je molekularnim markerima određen pol izleglih jedinki u periodu od 2013-2022 godine. Bez obzira na skorašnji drastičan pad brojnosti, nivo genetičke varijabilnosti procenjen na osnovu izabranih markera sličan je ostalim analiziranim populacijama u svetu. Analiza mikrosatelitskih lokusa je pokazala da populacija beloglavog supa iz Srbije poseduje najveći broj privatnih haplotipova, ali i nešto veći nivo inbridinga (8,3%). Detektovana su tri CytB haplotipa (jedan koji je prisutan u svim populacijama i dva karakteristična samo za populaciju iz Srbije). Na osnovu svih analiziranih markera, možemo reći da populacija supova u Srbiji poseduje genetičke osobenosti koje je jasno odvajaju od svih ostalih do sada analiziranih populacija kao i da je pokazan jednak odnos polova dodatni indikator stabilnosti i dobrog „zdravlja“ ove populacije. Populaciono genetičke analize pokazuju da je populacija beloglavog supa sa teritorije Srbije optimalni izbor za buduće programe reintrodukcije u regionu, uz napomenu da bi trebalo izbegavati reintrodukciju jedinki poreklom iz drugih geografskih područja.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Genetička varijabilnost populacije beloglavog supa na teritoriji Srbije – perspektiva za buduće programe reintrodukcije u regionu",
pages = "89",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5040"
}

Tanasković, M., Marinković, S., Hribšek, I., Patenković, A., Anđus, S., Stamenković-Radak, M.,& Davidović, S.. (2022). Genetička varijabilnost populacije beloglavog supa na teritoriji Srbije – perspektiva za buduće programe reintrodukcije u regionu. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 89.
https://hdl.handle.net/21.15107/rcub_ibiss_5040

Tanasković M, Marinković S, Hribšek I, Patenković A, Anđus S, Stamenković-Radak M, Davidović S. Genetička varijabilnost populacije beloglavog supa na teritoriji Srbije – perspektiva za buduće programe reintrodukcije u regionu. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:89.
https://hdl.handle.net/21.15107/rcub_ibiss_5040 .

Tanasković, Marija, Marinković, Saša, Hribšek, Irena, Patenković, Aleksandra, Anđus, Stefan, Stamenković-Radak, Marina, Davidović, Slobodan, "Genetička varijabilnost populacije beloglavog supa na teritoriji Srbije – perspektiva za buduće programe reintrodukcije u regionu" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):89,
https://hdl.handle.net/21.15107/rcub_ibiss_5040 .

TY  - JOUR
AU  - Erić, Pavle
AU  - Patenković, Aleksandra
AU  - Erić, Katarina
AU  - Tanasković, Marija
AU  - Davidović, Slobodan
AU  - Rakić, Mina
AU  - Savić Veselinović, Marija
AU  - Stamenković-Radak, Marina
AU  - Jelić, Mihailo
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4892
AB  - The adaptive significance of sympatric mitochondrial (mtDNA) variation and the role of
selective mechanisms that maintain it are debated to this day. Isofemale lines of Drosophila obscura
collected from four populations were backcrossed within populations to construct experimental
lines, with all combinations of mtDNA Cyt b haplotypes and nuclear genetic backgrounds (nuDNA).
Individuals of both sexes from these lines were then subjected to four fitness assays (desiccation
resistance, developmental time, egg-to-adult viability and sex ratio) on two experimental temperatures to examine the role of temperature fluctuations and sex-specific selection, as well as the part
that interactions between the two genomes play in shaping mtDNA variation. The results varied
across populations and fitness components. In the majority of comparisons, they show that sympatric
mitochondrial variants affect fitness. However, their effect should be examined in light of interactions
with nuDNA, as mito-nuclear genotype was even more influential on fitness across all components.
We found both sex-specific and temperature-specific differences in mitochondrial and mito-nuclear
genotype ranks in all fitness components. The effect of temperature-specific selection was found to
be more prominent, especially in desiccation resistance. From the results of different components
tested, we can also infer that temperature-specific mito-nuclear interactions rather than sex-specific
selection on mito-nuclear genotypes have a more substantial role in preserving mtDNA variation in
this model species.
PB  - Basel: MDPI
T2  - Insects
T1  - Temperature-Specific and Sex-Specific Fitness Effects of Sympatric Mitochondrial and Mito-Nuclear Variation in Drosophila obscura
IS  - 2
VL  - 13
DO  - 10.3390/insects13020139
SP  - 139
ER  - 

@article{
author = "Erić, Pavle and Patenković, Aleksandra and Erić, Katarina and Tanasković, Marija and Davidović, Slobodan and Rakić, Mina and Savić Veselinović, Marija and Stamenković-Radak, Marina and Jelić, Mihailo",
year = "2022",
abstract = "The adaptive significance of sympatric mitochondrial (mtDNA) variation and the role of
selective mechanisms that maintain it are debated to this day. Isofemale lines of Drosophila obscura
collected from four populations were backcrossed within populations to construct experimental
lines, with all combinations of mtDNA Cyt b haplotypes and nuclear genetic backgrounds (nuDNA).
Individuals of both sexes from these lines were then subjected to four fitness assays (desiccation
resistance, developmental time, egg-to-adult viability and sex ratio) on two experimental temperatures to examine the role of temperature fluctuations and sex-specific selection, as well as the part
that interactions between the two genomes play in shaping mtDNA variation. The results varied
across populations and fitness components. In the majority of comparisons, they show that sympatric
mitochondrial variants affect fitness. However, their effect should be examined in light of interactions
with nuDNA, as mito-nuclear genotype was even more influential on fitness across all components.
We found both sex-specific and temperature-specific differences in mitochondrial and mito-nuclear
genotype ranks in all fitness components. The effect of temperature-specific selection was found to
be more prominent, especially in desiccation resistance. From the results of different components
tested, we can also infer that temperature-specific mito-nuclear interactions rather than sex-specific
selection on mito-nuclear genotypes have a more substantial role in preserving mtDNA variation in
this model species.",
publisher = "Basel: MDPI",
journal = "Insects",
title = "Temperature-Specific and Sex-Specific Fitness Effects of Sympatric Mitochondrial and Mito-Nuclear Variation in Drosophila obscura",
number = "2",
volume = "13",
doi = "10.3390/insects13020139",
pages = "139"
}

Erić, P., Patenković, A., Erić, K., Tanasković, M., Davidović, S., Rakić, M., Savić Veselinović, M., Stamenković-Radak, M.,& Jelić, M.. (2022). Temperature-Specific and Sex-Specific Fitness Effects of Sympatric Mitochondrial and Mito-Nuclear Variation in Drosophila obscura. in Insects
Basel: MDPI., 13(2), 139.
https://doi.org/10.3390/insects13020139

Erić P, Patenković A, Erić K, Tanasković M, Davidović S, Rakić M, Savić Veselinović M, Stamenković-Radak M, Jelić M. Temperature-Specific and Sex-Specific Fitness Effects of Sympatric Mitochondrial and Mito-Nuclear Variation in Drosophila obscura. in Insects. 2022;13(2):139.
doi:10.3390/insects13020139 .

Erić, Pavle, Patenković, Aleksandra, Erić, Katarina, Tanasković, Marija, Davidović, Slobodan, Rakić, Mina, Savić Veselinović, Marija, Stamenković-Radak, Marina, Jelić, Mihailo, "Temperature-Specific and Sex-Specific Fitness Effects of Sympatric Mitochondrial and Mito-Nuclear Variation in Drosophila obscura" in Insects, 13, no. 2 (2022):139,
https://doi.org/10.3390/insects13020139 . .

TY  - JOUR
AU  - Erić, Pavle
AU  - Stamenković-Radak, Marina
AU  - Dragićević, Milan
AU  - Kankare, Maaria
AU  - Wallace, Megan A.
AU  - Savić Veselinović, Marija
AU  - Jelić, Mihailo
PY  - 2022
UR  - http://www.eje.cz/doi/10.14411/eje.2022.011.html
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4872
AB  - Drosophila obscura is a common fruit fly that inhabits the temperate forests of Europe. While it is abundant in the north compared to other Drosophila, its density decreases southwards, where it is gradually replaced by other Drosophila species. This study describes variation in the mitochondrial Cyt b gene of D. obscura from several European populations. We observed a large number of haplotypes, together with the structuring of genetic variation. Genetic variation is higher in the west where O1 and related divergent haplotypes dominate. In the east, the O2 haplotype is most frequent, together with haplotypes that recently arose from it. In the central part of the species range, both O1 and O2 are equally present, along with many others. These data reveal signs of population expansions that probably happened earlier in the west, and more recently in the east. Though our conclusions are based on only one genetic marker, limiting the power of the analysis, the results imply either postglacial expansion from two unique sources or, more likely, eastwards stepping-stone expansion. This study adds important information on genetic variation and phylogeography to the obscure biology of D. obscura, a species that has the potential to become an interesting model in evolutionary biology and conservation genetics.
PB  - České Budějovice: Czech Academy of Sciences
T2  - European Journal of Entomology
T1  - Mitochondrial DNA variation of Drosophila obscura (Diptera: Drosophilidae) across Europe
VL  - 119
DO  - 10.14411/eje.2022.011
SP  - 99
EP  - 110
ER  - 

@article{
author = "Erić, Pavle and Stamenković-Radak, Marina and Dragićević, Milan and Kankare, Maaria and Wallace, Megan A. and Savić Veselinović, Marija and Jelić, Mihailo",
year = "2022",
abstract = "Drosophila obscura is a common fruit fly that inhabits the temperate forests of Europe. While it is abundant in the north compared to other Drosophila, its density decreases southwards, where it is gradually replaced by other Drosophila species. This study describes variation in the mitochondrial Cyt b gene of D. obscura from several European populations. We observed a large number of haplotypes, together with the structuring of genetic variation. Genetic variation is higher in the west where O1 and related divergent haplotypes dominate. In the east, the O2 haplotype is most frequent, together with haplotypes that recently arose from it. In the central part of the species range, both O1 and O2 are equally present, along with many others. These data reveal signs of population expansions that probably happened earlier in the west, and more recently in the east. Though our conclusions are based on only one genetic marker, limiting the power of the analysis, the results imply either postglacial expansion from two unique sources or, more likely, eastwards stepping-stone expansion. This study adds important information on genetic variation and phylogeography to the obscure biology of D. obscura, a species that has the potential to become an interesting model in evolutionary biology and conservation genetics.",
publisher = "České Budějovice: Czech Academy of Sciences",
journal = "European Journal of Entomology",
title = "Mitochondrial DNA variation of Drosophila obscura (Diptera: Drosophilidae) across Europe",
volume = "119",
doi = "10.14411/eje.2022.011",
pages = "99-110"
}

Erić, P., Stamenković-Radak, M., Dragićević, M., Kankare, M., Wallace, M. A., Savić Veselinović, M.,& Jelić, M.. (2022). Mitochondrial DNA variation of Drosophila obscura (Diptera: Drosophilidae) across Europe. in European Journal of Entomology
České Budějovice: Czech Academy of Sciences., 119, 99-110.
https://doi.org/10.14411/eje.2022.011

Erić P, Stamenković-Radak M, Dragićević M, Kankare M, Wallace MA, Savić Veselinović M, Jelić M. Mitochondrial DNA variation of Drosophila obscura (Diptera: Drosophilidae) across Europe. in European Journal of Entomology. 2022;119:99-110.
doi:10.14411/eje.2022.011 .

Erić, Pavle, Stamenković-Radak, Marina, Dragićević, Milan, Kankare, Maaria, Wallace, Megan A., Savić Veselinović, Marija, Jelić, Mihailo, "Mitochondrial DNA variation of Drosophila obscura (Diptera: Drosophilidae) across Europe" in European Journal of Entomology, 119 (2022):99-110,
https://doi.org/10.14411/eje.2022.011 . .

TY  - JOUR
AU  - Erić, Katarina
AU  - Patenković, Aleksandra
AU  - Erić, Pavle
AU  - Davidović, Slobodan
AU  - Savić Veselinović, Marija
AU  - Stamenković-Radak, Marina
AU  - Tanasković, Marija
PY  - 2022
UR  - https://www.mdpi.com/2075-4450/13/2/138
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4800
AB  - Global warming and climate change are affecting many insect species in numerous ways. These species can develop diverse mechanisms as a response to variable environmental conditions. The rise in mean and extreme temperatures due to global warming and the importance of the population’s ability to adapt to temperature stress will further increase. In this study, we investigated thermal stress response, which is considered to be one of the crucial elements of population fitness and survival in fast-changing environments. The dynamics and variation of thermal stress resistance traits in D. subobscura flies originating from two natural populations sampled from different altitudes were analysed. Three different temperature regimes (25 °C, 19 °C, and 16 °C) were used for the F1 progeny from both localities to establish six experimental groups and investigate stress resistance traits: desiccation resistance, heat knock-down resistance, starvation resistance, and chill-coma recovery time. We detected that laboratory thermal conditions and population origin may have an effect on the analysed traits, and that sex also significantly influences stress resistance. Individuals from the lower altitude reared at higher temperatures show inferior resistance to thermal shock.
PB  - Basel: MDPI
T2  - Insects
T1  - Stress Resistance Traits under Different Thermal Conditions in Drosophila subobscura from Two Altitudes
IS  - 2
VL  - 13
DO  - 10.3390/insects13020138
SP  - 138
ER  - 

@article{
author = "Erić, Katarina and Patenković, Aleksandra and Erić, Pavle and Davidović, Slobodan and Savić Veselinović, Marija and Stamenković-Radak, Marina and Tanasković, Marija",
year = "2022",
abstract = "Global warming and climate change are affecting many insect species in numerous ways. These species can develop diverse mechanisms as a response to variable environmental conditions. The rise in mean and extreme temperatures due to global warming and the importance of the population’s ability to adapt to temperature stress will further increase. In this study, we investigated thermal stress response, which is considered to be one of the crucial elements of population fitness and survival in fast-changing environments. The dynamics and variation of thermal stress resistance traits in D. subobscura flies originating from two natural populations sampled from different altitudes were analysed. Three different temperature regimes (25 °C, 19 °C, and 16 °C) were used for the F1 progeny from both localities to establish six experimental groups and investigate stress resistance traits: desiccation resistance, heat knock-down resistance, starvation resistance, and chill-coma recovery time. We detected that laboratory thermal conditions and population origin may have an effect on the analysed traits, and that sex also significantly influences stress resistance. Individuals from the lower altitude reared at higher temperatures show inferior resistance to thermal shock.",
publisher = "Basel: MDPI",
journal = "Insects",
title = "Stress Resistance Traits under Different Thermal Conditions in Drosophila subobscura from Two Altitudes",
number = "2",
volume = "13",
doi = "10.3390/insects13020138",
pages = "138"
}

Erić, K., Patenković, A., Erić, P., Davidović, S., Savić Veselinović, M., Stamenković-Radak, M.,& Tanasković, M.. (2022). Stress Resistance Traits under Different Thermal Conditions in Drosophila subobscura from Two Altitudes. in Insects
Basel: MDPI., 13(2), 138.
https://doi.org/10.3390/insects13020138

Erić K, Patenković A, Erić P, Davidović S, Savić Veselinović M, Stamenković-Radak M, Tanasković M. Stress Resistance Traits under Different Thermal Conditions in Drosophila subobscura from Two Altitudes. in Insects. 2022;13(2):138.
doi:10.3390/insects13020138 .

Erić, Katarina, Patenković, Aleksandra, Erić, Pavle, Davidović, Slobodan, Savić Veselinović, Marija, Stamenković-Radak, Marina, Tanasković, Marija, "Stress Resistance Traits under Different Thermal Conditions in Drosophila subobscura from Two Altitudes" in Insects, 13, no. 2 (2022):138,
https://doi.org/10.3390/insects13020138 . .

TY  - CONF
AU  - Rakić, Mina
AU  - Patenković, Aleksandra
AU  - Stamenković-Radak, Marina
AU  - Tanasković, Marija
AU  - Jelić, Mihailo
AU  - Lapčević, Simona
AU  - Savić Veselinović, Marija
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5496
AB  - Lead is one of the most abundant pollutants extensively used all through human history
and, with industrialization and exploitation of its ores, its accumulation in the environment
has increased over time. As a non-essential heavy metal with high persistence and
toxicity, lead has severely negative effects on all organisms, but the adaptation of
populations and species in contaminated environments can be through different
responses. Metallothioneins (Mtn) are involved in heavy metal detoxification as metalbinding
proteins present in all living species, with six isoforms found in
the Drosophila genus. It is presumed that some of them have higher specificity for certain
metal ions, such as Pb2+, but the question arises if there is a specific response related to
species, sex and developmental stage. To further corroborate the metallothioneins with
an affinity for lead ions, the expression of six Mtn genes (MtnA-F) was quantified by RTqPCR
in larvae and adult female and male flies of D. melanogaster and D.
subobscura reared on the standard medium and medium enriched with lead-acetate for
thirteen generations and lead bioaccumulation was measured in adult flies. Our
experimental design allows for the detection of variation in expression of Mtn genes in
response to lead exposure between species, sexes and developmental stages. Results
also show a correlation between Mtn gene expression and measured lead
bioaccumulation in adult male and female flies of both species.
PB  - European Society for Evolutionary Biology
C3  - Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Prague, Czech Republic
T1  - Lead induces sex and development related differential Mtn gene expression in two Drosophila species
SP  - 471
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5496
ER  - 

@conference{
author = "Rakić, Mina and Patenković, Aleksandra and Stamenković-Radak, Marina and Tanasković, Marija and Jelić, Mihailo and Lapčević, Simona and Savić Veselinović, Marija",
year = "2022",
abstract = "Lead is one of the most abundant pollutants extensively used all through human history
and, with industrialization and exploitation of its ores, its accumulation in the environment
has increased over time. As a non-essential heavy metal with high persistence and
toxicity, lead has severely negative effects on all organisms, but the adaptation of
populations and species in contaminated environments can be through different
responses. Metallothioneins (Mtn) are involved in heavy metal detoxification as metalbinding
proteins present in all living species, with six isoforms found in
the Drosophila genus. It is presumed that some of them have higher specificity for certain
metal ions, such as Pb2+, but the question arises if there is a specific response related to
species, sex and developmental stage. To further corroborate the metallothioneins with
an affinity for lead ions, the expression of six Mtn genes (MtnA-F) was quantified by RTqPCR
in larvae and adult female and male flies of D. melanogaster and D.
subobscura reared on the standard medium and medium enriched with lead-acetate for
thirteen generations and lead bioaccumulation was measured in adult flies. Our
experimental design allows for the detection of variation in expression of Mtn genes in
response to lead exposure between species, sexes and developmental stages. Results
also show a correlation between Mtn gene expression and measured lead
bioaccumulation in adult male and female flies of both species.",
publisher = "European Society for Evolutionary Biology",
journal = "Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Prague, Czech Republic",
title = "Lead induces sex and development related differential Mtn gene expression in two Drosophila species",
pages = "471",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5496"
}

Rakić, M., Patenković, A., Stamenković-Radak, M., Tanasković, M., Jelić, M., Lapčević, S.,& Savić Veselinović, M.. (2022). Lead induces sex and development related differential Mtn gene expression in two Drosophila species. in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Prague, Czech Republic
European Society for Evolutionary Biology., 471.
https://hdl.handle.net/21.15107/rcub_ibiss_5496

Rakić M, Patenković A, Stamenković-Radak M, Tanasković M, Jelić M, Lapčević S, Savić Veselinović M. Lead induces sex and development related differential Mtn gene expression in two Drosophila species. in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Prague, Czech Republic. 2022;:471.
https://hdl.handle.net/21.15107/rcub_ibiss_5496 .

Rakić, Mina, Patenković, Aleksandra, Stamenković-Radak, Marina, Tanasković, Marija, Jelić, Mihailo, Lapčević, Simona, Savić Veselinović, Marija, "Lead induces sex and development related differential Mtn gene expression in two Drosophila species" in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Prague, Czech Republic (2022):471,
https://hdl.handle.net/21.15107/rcub_ibiss_5496 .

TY  - CONF
AU  - Savić Veselinović, Marija
AU  - Rakić, Mina
AU  - Patenković, Aleksandra
AU  - Tanasković, Marija
AU  - Jelić, Mihailo
AU  - Stamenković-Radak, Marina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5495
AB  - Повећање концентрација тешких метала у животној средини код различитих
организама може довести до поремећаја равнотеже концентрација есенцијалних
метала, узрокујући њихову токсичност као и неесенцијални метали. Иако су многи
од механизама који су у основи одговора на овај тип стреса прилично проучени,
присутна је варијабилност међу таксономским групама. Једна од првих линија
одбране већине организама јесте повећана експресија гена за металотионеине чији
су протеински продукти кратки, богати цистеинима, којима могу везивати јоне
метала и на тај начин вршити детоксикацију. Међу инсектима постоје врсте које
имају само један ген за металотионеин, али и оне које имају већи број гена који су
настали дупликацијама током еволуције. Функционалност и специфичност у
везивању одређених метала металотионеинима још увек није потпуно истражена, с
обзиром да се новим бионформатичким анализама и даље откривају нови гени код
неких од њих. Код рода Drosophila је скоро откривен шести ген за металотионеине
(MtnF), који би на основу секвенце требало да кодира протеин богатији цистеином
у поређењу са преосталих пет (Mtn A, B, C, D, E). Функција ових гена је у нашем
истраживању испитивана код две врсте овог рода, при изложености третманима
различитим металима и њиховим концентрацијама, различитог трајања и у
различитим стадијумима развића. Резултати анализе експресије гена MtnF не
указују на претпостављену специфичност везивања одређених метала.
AB  - Povećanje koncentracija teških metala u životnoj sredini kod različitih organizama može dovesti do poremećaja ravnoteže koncentracija esencijalnih metala, uzrokujući njihovu toksičnost kao i neesencijalni metali. Iako su mnogi od mehanizama koji su u osnovi odgovora na ovaj tip stresa prilično proučeni, prisutna je varijabilnost među taksonomskim grupama. Jedna od prvih linija odbrane većine organizama jeste povećana ekspresija gena za metalotioneine čiji su proteinski produkti kratki, bogati cisteinima, kojima mogu vezivati jone metala i na taj način vršiti detoksikaciju. Među insektima postoje vrste koje imaju samo jedan gen za metalotionein, ali i one koje imaju veći broj gena koji su nastali duplikacijama tokom evolucije. Funkcionalnost i specifičnost u vezivanju određenih metala metalotioneinima još uvek nije potpuno istražena, s obzirom da se novim bionformatičkim analizama i dalje otkrivaju novi geni kod nekih od njih. Kod roda Drosophila je skoro otkriven šesti gen za metalotioneine (MtnF), koji bi na osnovu sekvence trebalo da kodira protein bogatiji cisteinom u poređenju sa preostalih pet (Mtn A, B, C, D, E). Funkcija ovih gena je u našem istraživanju ispitivana kod dve vrste ovog roda, pri izloženosti tretmanima različitim metalima i njihovim koncentracijama, različitog trajanja i u različitim stadijumima razvića. Rezultati analize ekspresije gena MtnF ne ukazuju na pretpostavljenu specifičnost vezivanja određenih metala.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Oдговор на стрес индукован металима код инсеката – еволуција и улога гена за металотионеине
T1  - Odgovor na stres indukovan metalima kod insekata – evolucija i uloga gena za metalotioneine
SP  - 88
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5495
ER  - 

@conference{
author = "Savić Veselinović, Marija and Rakić, Mina and Patenković, Aleksandra and Tanasković, Marija and Jelić, Mihailo and Stamenković-Radak, Marina",
year = "2022",
abstract = "Повећање концентрација тешких метала у животној средини код различитих
организама може довести до поремећаја равнотеже концентрација есенцијалних
метала, узрокујући њихову токсичност као и неесенцијални метали. Иако су многи
од механизама који су у основи одговора на овај тип стреса прилично проучени,
присутна је варијабилност међу таксономским групама. Једна од првих линија
одбране већине организама јесте повећана експресија гена за металотионеине чији
су протеински продукти кратки, богати цистеинима, којима могу везивати јоне
метала и на тај начин вршити детоксикацију. Међу инсектима постоје врсте које
имају само један ген за металотионеин, али и оне које имају већи број гена који су
настали дупликацијама током еволуције. Функционалност и специфичност у
везивању одређених метала металотионеинима још увек није потпуно истражена, с
обзиром да се новим бионформатичким анализама и даље откривају нови гени код
неких од њих. Код рода Drosophila је скоро откривен шести ген за металотионеине
(MtnF), који би на основу секвенце требало да кодира протеин богатији цистеином
у поређењу са преосталих пет (Mtn A, B, C, D, E). Функција ових гена је у нашем
истраживању испитивана код две врсте овог рода, при изложености третманима
различитим металима и њиховим концентрацијама, различитог трајања и у
различитим стадијумима развића. Резултати анализе експресије гена MtnF не
указују на претпостављену специфичност везивања одређених метала., Povećanje koncentracija teških metala u životnoj sredini kod različitih organizama može dovesti do poremećaja ravnoteže koncentracija esencijalnih metala, uzrokujući njihovu toksičnost kao i neesencijalni metali. Iako su mnogi od mehanizama koji su u osnovi odgovora na ovaj tip stresa prilično proučeni, prisutna je varijabilnost među taksonomskim grupama. Jedna od prvih linija odbrane većine organizama jeste povećana ekspresija gena za metalotioneine čiji su proteinski produkti kratki, bogati cisteinima, kojima mogu vezivati jone metala i na taj način vršiti detoksikaciju. Među insektima postoje vrste koje imaju samo jedan gen za metalotionein, ali i one koje imaju veći broj gena koji su nastali duplikacijama tokom evolucije. Funkcionalnost i specifičnost u vezivanju određenih metala metalotioneinima još uvek nije potpuno istražena, s obzirom da se novim bionformatičkim analizama i dalje otkrivaju novi geni kod nekih od njih. Kod roda Drosophila je skoro otkriven šesti gen za metalotioneine (MtnF), koji bi na osnovu sekvence trebalo da kodira protein bogatiji cisteinom u poređenju sa preostalih pet (Mtn A, B, C, D, E). Funkcija ovih gena je u našem istraživanju ispitivana kod dve vrste ovog roda, pri izloženosti tretmanima različitim metalima i njihovim koncentracijama, različitog trajanja i u različitim stadijumima razvića. Rezultati analize ekspresije gena MtnF ne ukazuju na pretpostavljenu specifičnost vezivanja određenih metala.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Oдговор на стрес индукован металима код инсеката – еволуција и улога гена за металотионеине, Odgovor na stres indukovan metalima kod insekata – evolucija i uloga gena za metalotioneine",
pages = "88",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5495"
}

Savić Veselinović, M., Rakić, M., Patenković, A., Tanasković, M., Jelić, M.,& Stamenković-Radak, M.. (2022). Oдговор на стрес индукован металима код инсеката – еволуција и улога гена за металотионеине. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 88.
https://hdl.handle.net/21.15107/rcub_ibiss_5495

Savić Veselinović M, Rakić M, Patenković A, Tanasković M, Jelić M, Stamenković-Radak M. Oдговор на стрес индукован металима код инсеката – еволуција и улога гена за металотионеине. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:88.
https://hdl.handle.net/21.15107/rcub_ibiss_5495 .

Savić Veselinović, Marija, Rakić, Mina, Patenković, Aleksandra, Tanasković, Marija, Jelić, Mihailo, Stamenković-Radak, Marina, "Oдговор на стрес индукован металима код инсеката – еволуција и улога гена за металотионеине" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):88,
https://hdl.handle.net/21.15107/rcub_ibiss_5495 .

TY  - CONF
AU  - Erić, Katarina
AU  - Savić Veselinović, Marija
AU  - Patenković, Aleksandra
AU  - Erić, Pavle
AU  - Jelić, Miahilo
AU  - Stamenković-Radak, Marina
AU  - Tanasković, Marija
PY  - 2022
UR  - https://www.eseb2022.cz/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5095
AB  - Drosophila subobscura is a wide spread Palearctic species, which possesses very rich
inversion polymorphism in all of the five acrocentric chromosomes. As populations rapid
response to fast changes of environmental conditions can be seen through changes in
inversions frequency, this polymorphism is good candidate to measure the effect of
temperature on the genetic structure of population. Frequencies of particular
chromosomal arrangements are correlated with temperature, with standard
arrangements increasing in frequency at lower temperatures, and more complex
arrangements like J1, U1+2, U1+2+6, E1+2+9, and O3+4 increasing in frequency at higher
temperatures. In order to explore the effect of optimal and suboptimal temperatures on
the arrangements frequencies, samples of D. subobscura population from a high altitude
were reared in laboratory at suboptimal (16°C) and optimal (19°C). Chromosome
arrangements frequencies were analyzed and compared through generations (F0, F5, and
F16). The increasing frequency of complex chromosome arrangements and the
decreasing frequency of standard chromosomal arrangements were observed. There are
significant differences in some of the chromosome arrangements between suboptimal
and optimal conditions and also between generations, indicating laboratory adaptation
through generations.
PB  - European Society for Evolutionary Biology
PB  - e
C3  - Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic
T1  - Temperature shapes chromosome inversion polymorphism of Drosophila subobscura through generations
SP  - 759
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5095
ER  - 

@conference{
author = "Erić, Katarina and Savić Veselinović, Marija and Patenković, Aleksandra and Erić, Pavle and Jelić, Miahilo and Stamenković-Radak, Marina and Tanasković, Marija",
year = "2022",
abstract = "Drosophila subobscura is a wide spread Palearctic species, which possesses very rich
inversion polymorphism in all of the five acrocentric chromosomes. As populations rapid
response to fast changes of environmental conditions can be seen through changes in
inversions frequency, this polymorphism is good candidate to measure the effect of
temperature on the genetic structure of population. Frequencies of particular
chromosomal arrangements are correlated with temperature, with standard
arrangements increasing in frequency at lower temperatures, and more complex
arrangements like J1, U1+2, U1+2+6, E1+2+9, and O3+4 increasing in frequency at higher
temperatures. In order to explore the effect of optimal and suboptimal temperatures on
the arrangements frequencies, samples of D. subobscura population from a high altitude
were reared in laboratory at suboptimal (16°C) and optimal (19°C). Chromosome
arrangements frequencies were analyzed and compared through generations (F0, F5, and
F16). The increasing frequency of complex chromosome arrangements and the
decreasing frequency of standard chromosomal arrangements were observed. There are
significant differences in some of the chromosome arrangements between suboptimal
and optimal conditions and also between generations, indicating laboratory adaptation
through generations.",
publisher = "European Society for Evolutionary Biology, e",
journal = "Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic",
title = "Temperature shapes chromosome inversion polymorphism of Drosophila subobscura through generations",
pages = "759",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5095"
}

Erić, K., Savić Veselinović, M., Patenković, A., Erić, P., Jelić, M., Stamenković-Radak, M.,& Tanasković, M.. (2022). Temperature shapes chromosome inversion polymorphism of Drosophila subobscura through generations. in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic
European Society for Evolutionary Biology., 759.
https://hdl.handle.net/21.15107/rcub_ibiss_5095

Erić K, Savić Veselinović M, Patenković A, Erić P, Jelić M, Stamenković-Radak M, Tanasković M. Temperature shapes chromosome inversion polymorphism of Drosophila subobscura through generations. in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic. 2022;:759.
https://hdl.handle.net/21.15107/rcub_ibiss_5095 .

Erić, Katarina, Savić Veselinović, Marija, Patenković, Aleksandra, Erić, Pavle, Jelić, Miahilo, Stamenković-Radak, Marina, Tanasković, Marija, "Temperature shapes chromosome inversion polymorphism of Drosophila subobscura through generations" in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic (2022):759,
https://hdl.handle.net/21.15107/rcub_ibiss_5095 .

TY  - CONF
AU  - Davidović, Slobodan
AU  - Jelić, Mihailo
AU  - Marinković, Saša
AU  - Kukobat, Mila
AU  - Mihajlović, Milica
AU  - Tanasić, Vanja
AU  - Hribšek, Irena
AU  - Sušić, Goran
AU  - Dragićević, Milan
AU  - Tanasković, Marija
AU  - Stamenković-Radak, Marina
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4254
AB  - Abstract
Once a widespread species across the region of Southeast Europe, the Griffon vulture is now confined to small and isolated populations across the Balkan Peninsula. The population from Serbia represents its biggest and most viable population that can serve as an important reservoir of genetic diversity from which the birds can be used for the region’s reintroduction programs. The available genetic data for this valuable population are scarce and it is necessary to assess its genetic diversity and inbreeding level if the population is going to be used for restocking and reintroduction.
To assess the genetic diversity we used microsatellite markers from ten loci and mitochondrial Cytb nucleotide sequences. The blood samples were collected from 58 unrelated birds during the marking in the nests. We have performed a comparative analysis of newly obtained data on microsatellites and Cytb with existing data. Genetic differentiation analysis between different native populations of French Pyrenees, Croatia and Israel identified two genetic clusters that differentiate populations from the Balkan and Iberian Peninsulas. Genetic diversity analysis based on microsatellites demonstrated similar levels among all populations while analysis of Cytb detected somewhat lower diversity in the population from Serbia. Further analyses demonstrated that all analyzed populations experienced a recent bottleneck event. Phylogeographic analysis based on Cytb sequences showed that the most frequent haplotype is found in all Griffon vulture populations and that each population possesses private haplotypes. Considering the serious recent bottleneck event which the population from Serbia experienced we estimated the overall relatedness among the birds from this population. The level of inbreeding was relatively high and on average it was 8,3% while the mean number of relatives for each bird was close to three.
Our data suggest that, even though a relatively high level of inbreeding can be detected among the individual birds, the Griffon vulture population from Serbia can be used as a source population for restocking and reintroduction programs in the region. The observed genetic differentiation between the populations from the Iberian and Balkan Peninsula suggest that the introduction of foreign birds should be avoided and that local birds should be used instead.
PB  - Novi Sad: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad
C3  - Belgrade BioInformatics Conference 2021: Book of Abstracts; 2021 Jun 21-25; Vinča, Serbia
T1  - Genetic diversity analysis of microsatellites and mitochondrial Cytb gene, relatedness estimates and Cytb phylogeography of protected Griffon vulture species from Serbia
IS  - 1
VL  - 43
SP  - 117
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4254
ER  - 

@conference{
author = "Davidović, Slobodan and Jelić, Mihailo and Marinković, Saša and Kukobat, Mila and Mihajlović, Milica and Tanasić, Vanja and Hribšek, Irena and Sušić, Goran and Dragićević, Milan and Tanasković, Marija and Stamenković-Radak, Marina",
year = "2021",
abstract = "Abstract
Once a widespread species across the region of Southeast Europe, the Griffon vulture is now confined to small and isolated populations across the Balkan Peninsula. The population from Serbia represents its biggest and most viable population that can serve as an important reservoir of genetic diversity from which the birds can be used for the region’s reintroduction programs. The available genetic data for this valuable population are scarce and it is necessary to assess its genetic diversity and inbreeding level if the population is going to be used for restocking and reintroduction.
To assess the genetic diversity we used microsatellite markers from ten loci and mitochondrial Cytb nucleotide sequences. The blood samples were collected from 58 unrelated birds during the marking in the nests. We have performed a comparative analysis of newly obtained data on microsatellites and Cytb with existing data. Genetic differentiation analysis between different native populations of French Pyrenees, Croatia and Israel identified two genetic clusters that differentiate populations from the Balkan and Iberian Peninsulas. Genetic diversity analysis based on microsatellites demonstrated similar levels among all populations while analysis of Cytb detected somewhat lower diversity in the population from Serbia. Further analyses demonstrated that all analyzed populations experienced a recent bottleneck event. Phylogeographic analysis based on Cytb sequences showed that the most frequent haplotype is found in all Griffon vulture populations and that each population possesses private haplotypes. Considering the serious recent bottleneck event which the population from Serbia experienced we estimated the overall relatedness among the birds from this population. The level of inbreeding was relatively high and on average it was 8,3% while the mean number of relatives for each bird was close to three.
Our data suggest that, even though a relatively high level of inbreeding can be detected among the individual birds, the Griffon vulture population from Serbia can be used as a source population for restocking and reintroduction programs in the region. The observed genetic differentiation between the populations from the Iberian and Balkan Peninsula suggest that the introduction of foreign birds should be avoided and that local birds should be used instead.",
publisher = "Novi Sad: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad",
journal = "Belgrade BioInformatics Conference 2021: Book of Abstracts; 2021 Jun 21-25; Vinča, Serbia",
title = "Genetic diversity analysis of microsatellites and mitochondrial Cytb gene, relatedness estimates and Cytb phylogeography of protected Griffon vulture species from Serbia",
number = "1",
volume = "43",
pages = "117",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4254"
}

Davidović, S., Jelić, M., Marinković, S., Kukobat, M., Mihajlović, M., Tanasić, V., Hribšek, I., Sušić, G., Dragićević, M., Tanasković, M.,& Stamenković-Radak, M.. (2021). Genetic diversity analysis of microsatellites and mitochondrial Cytb gene, relatedness estimates and Cytb phylogeography of protected Griffon vulture species from Serbia. in Belgrade BioInformatics Conference 2021: Book of Abstracts; 2021 Jun 21-25; Vinča, Serbia
Novi Sad: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad., 43(1), 117.
https://hdl.handle.net/21.15107/rcub_ibiss_4254

Davidović S, Jelić M, Marinković S, Kukobat M, Mihajlović M, Tanasić V, Hribšek I, Sušić G, Dragićević M, Tanasković M, Stamenković-Radak M. Genetic diversity analysis of microsatellites and mitochondrial Cytb gene, relatedness estimates and Cytb phylogeography of protected Griffon vulture species from Serbia. in Belgrade BioInformatics Conference 2021: Book of Abstracts; 2021 Jun 21-25; Vinča, Serbia. 2021;43(1):117.
https://hdl.handle.net/21.15107/rcub_ibiss_4254 .

Davidović, Slobodan, Jelić, Mihailo, Marinković, Saša, Kukobat, Mila, Mihajlović, Milica, Tanasić, Vanja, Hribšek, Irena, Sušić, Goran, Dragićević, Milan, Tanasković, Marija, Stamenković-Radak, Marina, "Genetic diversity analysis of microsatellites and mitochondrial Cytb gene, relatedness estimates and Cytb phylogeography of protected Griffon vulture species from Serbia" in Belgrade BioInformatics Conference 2021: Book of Abstracts; 2021 Jun 21-25; Vinča, Serbia, 43, no. 1 (2021):117,
https://hdl.handle.net/21.15107/rcub_ibiss_4254 .

TY  - JOUR
AU  - Wallace, Megan A
AU  - Coffman, Kelsey A
AU  - Gilbert, Clément
AU  - Ravindran, Sanjana
AU  - Albery, Gregory F
AU  - Abbott, Jessica
AU  - Argyridou, Eliza
AU  - Bellosta, Paola
AU  - Betancourt, Andrea J
AU  - Colinet, Hervé
AU  - Eric, Katarina
AU  - Glaser-Schmitt, Amanda
AU  - Grath, Sonja
AU  - Jelić, Mihailo
AU  - Kankare, Maaria
AU  - Kozeretska, Iryna
AU  - Loeschcke, Volker
AU  - Montchamp-Moreau, Catherine
AU  - Ometto, Lino
AU  - Onder, Banu Sebnem
AU  - Orengo, Dorcas J
AU  - Parsch, John
AU  - Pascual, Marta
AU  - Patenković, Aleksandra
AU  - Puerma, Eva
AU  - Ritchie, Michael G
AU  - Rota-Stabelli, Omar
AU  - Schou, Mads Fristrup
AU  - Serga, Svitlana V
AU  - Stamenković-Radak, Marina
AU  - Tanasković, Marija
AU  - Savić Veselinović, Marija
AU  - Vieira, Jorge
AU  - Vieira, Cristina P
AU  - Kapun, Martin
AU  - Flatt, Thomas
AU  - González, Josefa
AU  - Staubach, Fabian
AU  - Obbard, Darren J
PY  - 2021
UR  - https://academic.oup.com/ve/advance-article/doi/10.1093/ve/veab031/6207981
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4184
AB  - Drosophila melanogaster is an important model for antiviral immunity in arthropods, but very few DNA viruses have been described from the family Drosophilidae. This deficiency limits our opportunity to use natural host-pathogen combinations in experimental studies, and may bias our understanding of the Drosophila virome. Here we report fourteen DNA viruses detected in a metagenomic analysis of approximately 6500 pool-sequenced Drosophila, sampled from 47 European locations between 2014 and 2016. These include three new nudiviruses, a new and divergent entomopoxvirus, a virus related to Leptopilina boulardi filamentous virus, and a virus related to Musca domestica salivary gland hypertrophy virus. We also find an endogenous genomic copy of galbut virus, a dsRNA partitivirus, segregating at very low frequency. Remarkably, we find that Drosophila Vesanto virus, a small DNA virus previously described as a bidnavirus, may be composed of up to 12 segments and thus represent a new lineage of segmented DNA viruses. Two of the DNA viruses, Drosophila Kallithea nudivirus and Drosophila Vesanto virus are relatively common, found in 2% or more of wild flies. The others are rare, with many likely to be represented by a single infected fly. We find that virus prevalence in Europe reflects the prevalence seen in publicly-available datasets, with Drosophila Kallithea nudivirus and Drosophila Vesanto virus the only ones commonly detectable in public data from wild-caught flies and large population cages, and the other viruses being rare or absent. These analyses suggest that DNA viruses are at lower prevalence than RNA viruses in D. melanogaster, and may be less likely to persist in laboratory cultures. Our findings go some way to redressing an earlier bias toward RNA virus studies in Drosophila, and lay the foundation needed to harness the power of Drosophila as a model system for the study of DNA viruses.
T2  - Virus Evolution
T2  - Virus Evolution
T1  - The discovery, distribution and diversity of DNA viruses associated with Drosophila melanogaster in Europe
DO  - 10.1093/ve/veab031
ER  - 

@article{
author = "Wallace, Megan A and Coffman, Kelsey A and Gilbert, Clément and Ravindran, Sanjana and Albery, Gregory F and Abbott, Jessica and Argyridou, Eliza and Bellosta, Paola and Betancourt, Andrea J and Colinet, Hervé and Eric, Katarina and Glaser-Schmitt, Amanda and Grath, Sonja and Jelić, Mihailo and Kankare, Maaria and Kozeretska, Iryna and Loeschcke, Volker and Montchamp-Moreau, Catherine and Ometto, Lino and Onder, Banu Sebnem and Orengo, Dorcas J and Parsch, John and Pascual, Marta and Patenković, Aleksandra and Puerma, Eva and Ritchie, Michael G and Rota-Stabelli, Omar and Schou, Mads Fristrup and Serga, Svitlana V and Stamenković-Radak, Marina and Tanasković, Marija and Savić Veselinović, Marija and Vieira, Jorge and Vieira, Cristina P and Kapun, Martin and Flatt, Thomas and González, Josefa and Staubach, Fabian and Obbard, Darren J",
year = "2021",
abstract = "Drosophila melanogaster is an important model for antiviral immunity in arthropods, but very few DNA viruses have been described from the family Drosophilidae. This deficiency limits our opportunity to use natural host-pathogen combinations in experimental studies, and may bias our understanding of the Drosophila virome. Here we report fourteen DNA viruses detected in a metagenomic analysis of approximately 6500 pool-sequenced Drosophila, sampled from 47 European locations between 2014 and 2016. These include three new nudiviruses, a new and divergent entomopoxvirus, a virus related to Leptopilina boulardi filamentous virus, and a virus related to Musca domestica salivary gland hypertrophy virus. We also find an endogenous genomic copy of galbut virus, a dsRNA partitivirus, segregating at very low frequency. Remarkably, we find that Drosophila Vesanto virus, a small DNA virus previously described as a bidnavirus, may be composed of up to 12 segments and thus represent a new lineage of segmented DNA viruses. Two of the DNA viruses, Drosophila Kallithea nudivirus and Drosophila Vesanto virus are relatively common, found in 2% or more of wild flies. The others are rare, with many likely to be represented by a single infected fly. We find that virus prevalence in Europe reflects the prevalence seen in publicly-available datasets, with Drosophila Kallithea nudivirus and Drosophila Vesanto virus the only ones commonly detectable in public data from wild-caught flies and large population cages, and the other viruses being rare or absent. These analyses suggest that DNA viruses are at lower prevalence than RNA viruses in D. melanogaster, and may be less likely to persist in laboratory cultures. Our findings go some way to redressing an earlier bias toward RNA virus studies in Drosophila, and lay the foundation needed to harness the power of Drosophila as a model system for the study of DNA viruses.",
journal = "Virus Evolution, Virus Evolution",
title = "The discovery, distribution and diversity of DNA viruses associated with Drosophila melanogaster in Europe",
doi = "10.1093/ve/veab031"
}

Wallace, M. A., Coffman, K. A., Gilbert, C., Ravindran, S., Albery, G. F., Abbott, J., Argyridou, E., Bellosta, P., Betancourt, A. J., Colinet, H., Eric, K., Glaser-Schmitt, A., Grath, S., Jelić, M., Kankare, M., Kozeretska, I., Loeschcke, V., Montchamp-Moreau, C., Ometto, L., Onder, B. S., Orengo, D. J., Parsch, J., Pascual, M., Patenković, A., Puerma, E., Ritchie, M. G., Rota-Stabelli, O., Schou, M. F., Serga, S. V., Stamenković-Radak, M., Tanasković, M., Savić Veselinović, M., Vieira, J., Vieira, C. P., Kapun, M., Flatt, T., González, J., Staubach, F.,& Obbard, D. J.. (2021). The discovery, distribution and diversity of DNA viruses associated with Drosophila melanogaster in Europe. in Virus Evolution.
https://doi.org/10.1093/ve/veab031

Wallace MA, Coffman KA, Gilbert C, Ravindran S, Albery GF, Abbott J, Argyridou E, Bellosta P, Betancourt AJ, Colinet H, Eric K, Glaser-Schmitt A, Grath S, Jelić M, Kankare M, Kozeretska I, Loeschcke V, Montchamp-Moreau C, Ometto L, Onder BS, Orengo DJ, Parsch J, Pascual M, Patenković A, Puerma E, Ritchie MG, Rota-Stabelli O, Schou MF, Serga SV, Stamenković-Radak M, Tanasković M, Savić Veselinović M, Vieira J, Vieira CP, Kapun M, Flatt T, González J, Staubach F, Obbard DJ. The discovery, distribution and diversity of DNA viruses associated with Drosophila melanogaster in Europe. in Virus Evolution. 2021;.
doi:10.1093/ve/veab031 .

Wallace, Megan A, Coffman, Kelsey A, Gilbert, Clément, Ravindran, Sanjana, Albery, Gregory F, Abbott, Jessica, Argyridou, Eliza, Bellosta, Paola, Betancourt, Andrea J, Colinet, Hervé, Eric, Katarina, Glaser-Schmitt, Amanda, Grath, Sonja, Jelić, Mihailo, Kankare, Maaria, Kozeretska, Iryna, Loeschcke, Volker, Montchamp-Moreau, Catherine, Ometto, Lino, Onder, Banu Sebnem, Orengo, Dorcas J, Parsch, John, Pascual, Marta, Patenković, Aleksandra, Puerma, Eva, Ritchie, Michael G, Rota-Stabelli, Omar, Schou, Mads Fristrup, Serga, Svitlana V, Stamenković-Radak, Marina, Tanasković, Marija, Savić Veselinović, Marija, Vieira, Jorge, Vieira, Cristina P, Kapun, Martin, Flatt, Thomas, González, Josefa, Staubach, Fabian, Obbard, Darren J, "The discovery, distribution and diversity of DNA viruses associated with Drosophila melanogaster in Europe" in Virus Evolution (2021),
https://doi.org/10.1093/ve/veab031 . .

TY  - JOUR
AU  - Kim, Bernard Y
AU  - Wang, Jeremy R
AU  - Miller, Danny E
AU  - Barmina, Olga
AU  - Delaney, Emily
AU  - Thompson, Ammon
AU  - Comeault, Aaron A
AU  - Peede, David
AU  - D'Agostino, Emmanuel RR
AU  - Pelaez, Julianne
AU  - Aguilar, Jessica M
AU  - Haji, Diler
AU  - Matsunaga, Teruyuki
AU  - Armstrong, Ellie E
AU  - Zych, Molly
AU  - Ogawa, Yoshitaka
AU  - Stamenković-Radak, Marina
AU  - Jelić, Mihailo
AU  - Savić Veselinović, Marija
AU  - Tanasković, Marija
AU  - Erić, Pavle
AU  - Gao, Jian-Jun
AU  - Katoh, Takehiro K
AU  - Toda, Masanori J
AU  - Watabe, Hideaki
AU  - Watada, Masayoshi
AU  - Davis, Jeremy S
AU  - Moyle, Leonie C
AU  - Manoli, Giulia
AU  - Bertolini, Enrico
AU  - Košťál, Vladimír
AU  - Hawley, R Scott
AU  - Takahashi, Aya
AU  - Jones, Corbin D
AU  - Price, Donald K
AU  - Whiteman, Noah
AU  - Kopp, Artyom
AU  - Matute, Daniel R
AU  - Petrov, Dmitri A
PY  - 2021
UR  - https://elifesciences.org/articles/66405
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8337076
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4457
AB  - Over 100 years of studies in Drosophila melanogaster and related species in the genus Drosophila have facilitated key discoveries in genetics, genomics, and evolution. While high-quality genome assemblies exist for several species in this group, they only encompass a small fraction of the genus. Recent advances in long-read sequencing allow high-quality genome assemblies for tens or even hundreds of species to be efficiently generated. Here, we utilize Oxford Nanopore sequencing to build an open community resource of genome assemblies for 101 lines of 93 drosophilid species encompassing 14 species groups and 35 sub-groups. The genomes are highly contiguous and complete, with an average contig N50 of 10.5 Mb and greater than 97% BUSCO completeness in 97/101 assemblies. We show that Nanopore-based assemblies are highly accurate in coding regions, particularly with respect to coding insertions and deletions. These assemblies, along with a detailed laboratory protocol and assembly pipelines, are released as a public resource and will serve as a starting point for addressing broad questions of genetics, ecology, and evolution at the scale of hundreds of species.
PB  - Cambridge: eLife Sciences Publications Ltd
T2  - eLife
T1  - Highly contiguous assemblies of 101 drosophilid genomes.
VL  - 10
DO  - 10.7554/eLife.66405
SP  - e66405
ER  - 

@article{
author = "Kim, Bernard Y and Wang, Jeremy R and Miller, Danny E and Barmina, Olga and Delaney, Emily and Thompson, Ammon and Comeault, Aaron A and Peede, David and D'Agostino, Emmanuel RR and Pelaez, Julianne and Aguilar, Jessica M and Haji, Diler and Matsunaga, Teruyuki and Armstrong, Ellie E and Zych, Molly and Ogawa, Yoshitaka and Stamenković-Radak, Marina and Jelić, Mihailo and Savić Veselinović, Marija and Tanasković, Marija and Erić, Pavle and Gao, Jian-Jun and Katoh, Takehiro K and Toda, Masanori J and Watabe, Hideaki and Watada, Masayoshi and Davis, Jeremy S and Moyle, Leonie C and Manoli, Giulia and Bertolini, Enrico and Košťál, Vladimír and Hawley, R Scott and Takahashi, Aya and Jones, Corbin D and Price, Donald K and Whiteman, Noah and Kopp, Artyom and Matute, Daniel R and Petrov, Dmitri A",
year = "2021",
abstract = "Over 100 years of studies in Drosophila melanogaster and related species in the genus Drosophila have facilitated key discoveries in genetics, genomics, and evolution. While high-quality genome assemblies exist for several species in this group, they only encompass a small fraction of the genus. Recent advances in long-read sequencing allow high-quality genome assemblies for tens or even hundreds of species to be efficiently generated. Here, we utilize Oxford Nanopore sequencing to build an open community resource of genome assemblies for 101 lines of 93 drosophilid species encompassing 14 species groups and 35 sub-groups. The genomes are highly contiguous and complete, with an average contig N50 of 10.5 Mb and greater than 97% BUSCO completeness in 97/101 assemblies. We show that Nanopore-based assemblies are highly accurate in coding regions, particularly with respect to coding insertions and deletions. These assemblies, along with a detailed laboratory protocol and assembly pipelines, are released as a public resource and will serve as a starting point for addressing broad questions of genetics, ecology, and evolution at the scale of hundreds of species.",
publisher = "Cambridge: eLife Sciences Publications Ltd",
journal = "eLife",
title = "Highly contiguous assemblies of 101 drosophilid genomes.",
volume = "10",
doi = "10.7554/eLife.66405",
pages = "e66405"
}

Kim, B. Y., Wang, J. R., Miller, D. E., Barmina, O., Delaney, E., Thompson, A., Comeault, A. A., Peede, D., D'Agostino, E. R., Pelaez, J., Aguilar, J. M., Haji, D., Matsunaga, T., Armstrong, E. E., Zych, M., Ogawa, Y., Stamenković-Radak, M., Jelić, M., Savić Veselinović, M., Tanasković, M., Erić, P., Gao, J., Katoh, T. K., Toda, M. J., Watabe, H., Watada, M., Davis, J. S., Moyle, L. C., Manoli, G., Bertolini, E., Košťál, V., Hawley, R. S., Takahashi, A., Jones, C. D., Price, D. K., Whiteman, N., Kopp, A., Matute, D. R.,& Petrov, D. A.. (2021). Highly contiguous assemblies of 101 drosophilid genomes.. in eLife
Cambridge: eLife Sciences Publications Ltd., 10, e66405.
https://doi.org/10.7554/eLife.66405

Kim BY, Wang JR, Miller DE, Barmina O, Delaney E, Thompson A, Comeault AA, Peede D, D'Agostino ER, Pelaez J, Aguilar JM, Haji D, Matsunaga T, Armstrong EE, Zych M, Ogawa Y, Stamenković-Radak M, Jelić M, Savić Veselinović M, Tanasković M, Erić P, Gao J, Katoh TK, Toda MJ, Watabe H, Watada M, Davis JS, Moyle LC, Manoli G, Bertolini E, Košťál V, Hawley RS, Takahashi A, Jones CD, Price DK, Whiteman N, Kopp A, Matute DR, Petrov DA. Highly contiguous assemblies of 101 drosophilid genomes.. in eLife. 2021;10:e66405.
doi:10.7554/eLife.66405 .

Kim, Bernard Y, Wang, Jeremy R, Miller, Danny E, Barmina, Olga, Delaney, Emily, Thompson, Ammon, Comeault, Aaron A, Peede, David, D'Agostino, Emmanuel RR, Pelaez, Julianne, Aguilar, Jessica M, Haji, Diler, Matsunaga, Teruyuki, Armstrong, Ellie E, Zych, Molly, Ogawa, Yoshitaka, Stamenković-Radak, Marina, Jelić, Mihailo, Savić Veselinović, Marija, Tanasković, Marija, Erić, Pavle, Gao, Jian-Jun, Katoh, Takehiro K, Toda, Masanori J, Watabe, Hideaki, Watada, Masayoshi, Davis, Jeremy S, Moyle, Leonie C, Manoli, Giulia, Bertolini, Enrico, Košťál, Vladimír, Hawley, R Scott, Takahashi, Aya, Jones, Corbin D, Price, Donald K, Whiteman, Noah, Kopp, Artyom, Matute, Daniel R, Petrov, Dmitri A, "Highly contiguous assemblies of 101 drosophilid genomes." in eLife, 10 (2021):e66405,
https://doi.org/10.7554/eLife.66405 . .

TY  - JOUR
AU  - Beribaka, Mirjana
AU  - Jelić, Mihailo
AU  - Tanasković, Marija
AU  - Lazić, Cvijeta
AU  - Stamenković-Radak, Marina
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4750
AB  - Life history traits determine the persistence and reproduction of each species. Factors that can affect life history traits are numerous and can be of different origin. We investigated the influence of population origin and heavy metal exposure on microbiota diversity and two life history traits, egg-to-adult viability and developmental time, in Drosophila melanogaster and Drosophila subobscura, grown in the laboratory on a lead (II) acetate-saturated substrate. We used 24 samples, 8 larval and 16 adult samples (two species × two substrates × two populations × two sexes). The composition of microbiota was determined by sequencing (NGS) of the V3–V4 variable regions of the 16S rRNA gene. The population origin showed a significant influence on life history traits, though each trait in the two species was affected differentially. Reduced viability in D. melanogaster could be a cost of fast development, decrease in Lactobacillus abundance and the presence of Wolbachia. The heavy metal exposure in D. subobscura caused shifts in developmental time but maintained the egg-to-adult viability at a similar level. Microbiota diversity indicated that the Komagataeibacter could be a valuable member of D. subobscura microbiota in overcoming the environmental stress. Research on the impact of microbiota on the adaptive response to heavy metals and consequently the potential tradeoffs among different life history traits is of great importance in evolutionary research.
PB  - Basel: MDPI
T2  - Insects
T1  - Life History Traits in Two Drosophila Species Differently Affected by Microbiota Diversity under Lead Exposure
IS  - 12
VL  - 12
DO  - 10.3390/insects12121122
SP  - 1122
ER  - 

@article{
author = "Beribaka, Mirjana and Jelić, Mihailo and Tanasković, Marija and Lazić, Cvijeta and Stamenković-Radak, Marina",
year = "2021",
abstract = "Life history traits determine the persistence and reproduction of each species. Factors that can affect life history traits are numerous and can be of different origin. We investigated the influence of population origin and heavy metal exposure on microbiota diversity and two life history traits, egg-to-adult viability and developmental time, in Drosophila melanogaster and Drosophila subobscura, grown in the laboratory on a lead (II) acetate-saturated substrate. We used 24 samples, 8 larval and 16 adult samples (two species × two substrates × two populations × two sexes). The composition of microbiota was determined by sequencing (NGS) of the V3–V4 variable regions of the 16S rRNA gene. The population origin showed a significant influence on life history traits, though each trait in the two species was affected differentially. Reduced viability in D. melanogaster could be a cost of fast development, decrease in Lactobacillus abundance and the presence of Wolbachia. The heavy metal exposure in D. subobscura caused shifts in developmental time but maintained the egg-to-adult viability at a similar level. Microbiota diversity indicated that the Komagataeibacter could be a valuable member of D. subobscura microbiota in overcoming the environmental stress. Research on the impact of microbiota on the adaptive response to heavy metals and consequently the potential tradeoffs among different life history traits is of great importance in evolutionary research.",
publisher = "Basel: MDPI",
journal = "Insects",
title = "Life History Traits in Two Drosophila Species Differently Affected by Microbiota Diversity under Lead Exposure",
number = "12",
volume = "12",
doi = "10.3390/insects12121122",
pages = "1122"
}

Beribaka, M., Jelić, M., Tanasković, M., Lazić, C.,& Stamenković-Radak, M.. (2021). Life History Traits in Two Drosophila Species Differently Affected by Microbiota Diversity under Lead Exposure. in Insects
Basel: MDPI., 12(12), 1122.
https://doi.org/10.3390/insects12121122

Beribaka M, Jelić M, Tanasković M, Lazić C, Stamenković-Radak M. Life History Traits in Two Drosophila Species Differently Affected by Microbiota Diversity under Lead Exposure. in Insects. 2021;12(12):1122.
doi:10.3390/insects12121122 .

Beribaka, Mirjana, Jelić, Mihailo, Tanasković, Marija, Lazić, Cvijeta, Stamenković-Radak, Marina, "Life History Traits in Two Drosophila Species Differently Affected by Microbiota Diversity under Lead Exposure" in Insects, 12, no. 12 (2021):1122,
https://doi.org/10.3390/insects12121122 . .

TY  - JOUR
AU  - Kapun, Martin
AU  - Nunez, Joaquin C B
AU  - Bogaerts-Márquez, María
AU  - Murga-Moreno, Jesús
AU  - Paris, Margot
AU  - Outten, Joseph
AU  - Coronado-Zamora, Marta
AU  - Tern, Courtney
AU  - Rota-Stabelli, Omar
AU  - Guerreiro, Maria P García
AU  - Casillas, Sònia
AU  - Orengo, Dorcas J
AU  - Puerma, Eva
AU  - Kankare, Maaria
AU  - Ometto, Lino
AU  - Loeschcke, Volker
AU  - Onder, Banu S
AU  - Abbott, Jessica K
AU  - Schaeffer, Stephen W
AU  - Rajpurohit, Subhash
AU  - Behrman, Emily L
AU  - Schou, Mads F
AU  - Merritt, Thomas J S
AU  - Lazzaro, Brian P
AU  - Glaser-Schmitt, Amanda
AU  - Argyridou, Eliza
AU  - Staubach, Fabian
AU  - Wang, Yun
AU  - Tauber, Eran
AU  - Serga, Svitlana V
AU  - Fabian, Daniel K
AU  - Dyer, Kelly A
AU  - Wheat, Christopher W
AU  - Parsch, John
AU  - Grath, Sonja
AU  - Savić Veselinović, Marija
AU  - Stamenković-Radak, Marina
AU  - Jelić, Mihailo
AU  - Buendía-Ruíz, Antonio J
AU  - Gómez-Julián, Maria Josefa
AU  - Espinosa-Jimenez, Maria Luisa
AU  - Gallardo-Jiménez, Francisco D
AU  - Patenković, Aleksandra
AU  - Erić, Katarina
AU  - Tanasković, Marija
AU  - Ullastres, Anna
AU  - Guio, Lain
AU  - Merenciano, Miriam
AU  - Guirao-Rico, Sara
AU  - Horváth, Vivien
AU  - Obbard, Darren J
AU  - Pasyukova, Elena
AU  - Alatortsev, Vladimir E
AU  - Vieira, Cristina P
AU  - Vieira, Jorge
AU  - Torres, Jorge Roberto
AU  - Kozeretska, Iryna
AU  - Maistrenko, Oleksandr M
AU  - Montchamp-Moreau, Catherine
AU  - Mukha, Dmitry V
AU  - Machado, Heather E
AU  - Lamb, Keric
AU  - Paulo, Tânia
AU  - Yusuf, Leeban
AU  - Barbadilla, Antonio
AU  - Petrov, Dmitri
AU  - Schmidt, Paul
AU  - Gonzalez, Josefa
AU  - Flatt, Thomas
AU  - Bergland, Alan O
AU  - Nielsen, Rasmus
PY  - 2021
UR  - https://academic.oup.com/mbe/article/38/12/5782/6361628
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4765
AB  - Drosophila melanogaster is a leading model in population genetics and genomics, and a growing number of whole-genome data sets from natural populations of this species have been published over the last years. A major challenge is the integration of disparate data sets, often generated using different sequencing technologies and bioinformatic pipelines, which hampers our ability to address questions about the evolution of this species. Here we address these issues by developing a bioinformatics pipeline that maps pooled sequencing (Pool-Seq) reads from D. melanogaster to a hologenome consisting of fly and symbiont genomes and estimates allele frequencies using either a heuristic (PoolSNP) or a probabilistic variant caller (SNAPE-pooled). We use this pipeline to generate the largest data repository of genomic data available for D. melanogaster to date, encompassing 271 previously published and unpublished population samples from over 100 locations in >20 countries on four continents. Several of these locations have been sampled at different seasons across multiple years. This data set, which we call Drosophila Evolution over Space and Time (DEST), is coupled with sampling and environmental metadata. A web-based genome browser and web portal provide easy access to the SNP data set. We further provide guidelines on how to use Pool-Seq data for model-based demographic inference. Our aim is to provide this scalable platform as a community resource which can be easily extended via future efforts for an even more extensive cosmopolitan data set. Our resource will enable population geneticists to analyze spatiotemporal genetic patterns and evolutionary dynamics of D. melanogaster populations in unprecedented detail.
PB  - Oxford: Oxford University Press
T2  - Molecular Biology and Evolution
T1  - Drosophila Evolution over Space and Time (DEST): A New Population Genomics Resource
IS  - 12
VL  - 38
DO  - 10.1093/molbev/msab259
SP  - 5782
EP  - 5805
ER  - 

@article{
author = "Kapun, Martin and Nunez, Joaquin C B and Bogaerts-Márquez, María and Murga-Moreno, Jesús and Paris, Margot and Outten, Joseph and Coronado-Zamora, Marta and Tern, Courtney and Rota-Stabelli, Omar and Guerreiro, Maria P García and Casillas, Sònia and Orengo, Dorcas J and Puerma, Eva and Kankare, Maaria and Ometto, Lino and Loeschcke, Volker and Onder, Banu S and Abbott, Jessica K and Schaeffer, Stephen W and Rajpurohit, Subhash and Behrman, Emily L and Schou, Mads F and Merritt, Thomas J S and Lazzaro, Brian P and Glaser-Schmitt, Amanda and Argyridou, Eliza and Staubach, Fabian and Wang, Yun and Tauber, Eran and Serga, Svitlana V and Fabian, Daniel K and Dyer, Kelly A and Wheat, Christopher W and Parsch, John and Grath, Sonja and Savić Veselinović, Marija and Stamenković-Radak, Marina and Jelić, Mihailo and Buendía-Ruíz, Antonio J and Gómez-Julián, Maria Josefa and Espinosa-Jimenez, Maria Luisa and Gallardo-Jiménez, Francisco D and Patenković, Aleksandra and Erić, Katarina and Tanasković, Marija and Ullastres, Anna and Guio, Lain and Merenciano, Miriam and Guirao-Rico, Sara and Horváth, Vivien and Obbard, Darren J and Pasyukova, Elena and Alatortsev, Vladimir E and Vieira, Cristina P and Vieira, Jorge and Torres, Jorge Roberto and Kozeretska, Iryna and Maistrenko, Oleksandr M and Montchamp-Moreau, Catherine and Mukha, Dmitry V and Machado, Heather E and Lamb, Keric and Paulo, Tânia and Yusuf, Leeban and Barbadilla, Antonio and Petrov, Dmitri and Schmidt, Paul and Gonzalez, Josefa and Flatt, Thomas and Bergland, Alan O and Nielsen, Rasmus",
year = "2021",
abstract = "Drosophila melanogaster is a leading model in population genetics and genomics, and a growing number of whole-genome data sets from natural populations of this species have been published over the last years. A major challenge is the integration of disparate data sets, often generated using different sequencing technologies and bioinformatic pipelines, which hampers our ability to address questions about the evolution of this species. Here we address these issues by developing a bioinformatics pipeline that maps pooled sequencing (Pool-Seq) reads from D. melanogaster to a hologenome consisting of fly and symbiont genomes and estimates allele frequencies using either a heuristic (PoolSNP) or a probabilistic variant caller (SNAPE-pooled). We use this pipeline to generate the largest data repository of genomic data available for D. melanogaster to date, encompassing 271 previously published and unpublished population samples from over 100 locations in >20 countries on four continents. Several of these locations have been sampled at different seasons across multiple years. This data set, which we call Drosophila Evolution over Space and Time (DEST), is coupled with sampling and environmental metadata. A web-based genome browser and web portal provide easy access to the SNP data set. We further provide guidelines on how to use Pool-Seq data for model-based demographic inference. Our aim is to provide this scalable platform as a community resource which can be easily extended via future efforts for an even more extensive cosmopolitan data set. Our resource will enable population geneticists to analyze spatiotemporal genetic patterns and evolutionary dynamics of D. melanogaster populations in unprecedented detail.",
publisher = "Oxford: Oxford University Press",
journal = "Molecular Biology and Evolution",
title = "Drosophila Evolution over Space and Time (DEST): A New Population Genomics Resource",
number = "12",
volume = "38",
doi = "10.1093/molbev/msab259",
pages = "5782-5805"
}

Kapun, M., Nunez, J. C. B., Bogaerts-Márquez, M., Murga-Moreno, J., Paris, M., Outten, J., Coronado-Zamora, M., Tern, C., Rota-Stabelli, O., Guerreiro, M. P. G., Casillas, S., Orengo, D. J., Puerma, E., Kankare, M., Ometto, L., Loeschcke, V., Onder, B. S., Abbott, J. K., Schaeffer, S. W., Rajpurohit, S., Behrman, E. L., Schou, M. F., Merritt, T. J. S., Lazzaro, B. P., Glaser-Schmitt, A., Argyridou, E., Staubach, F., Wang, Y., Tauber, E., Serga, S. V., Fabian, D. K., Dyer, K. A., Wheat, C. W., Parsch, J., Grath, S., Savić Veselinović, M., Stamenković-Radak, M., Jelić, M., Buendía-Ruíz, A. J., Gómez-Julián, M. J., Espinosa-Jimenez, M. L., Gallardo-Jiménez, F. D., Patenković, A., Erić, K., Tanasković, M., Ullastres, A., Guio, L., Merenciano, M., Guirao-Rico, S., Horváth, V., Obbard, D. J., Pasyukova, E., Alatortsev, V. E., Vieira, C. P., Vieira, J., Torres, J. R., Kozeretska, I., Maistrenko, O. M., Montchamp-Moreau, C., Mukha, D. V., Machado, H. E., Lamb, K., Paulo, T., Yusuf, L., Barbadilla, A., Petrov, D., Schmidt, P., Gonzalez, J., Flatt, T., Bergland, A. O.,& Nielsen, R.. (2021). Drosophila Evolution over Space and Time (DEST): A New Population Genomics Resource. in Molecular Biology and Evolution
Oxford: Oxford University Press., 38(12), 5782-5805.
https://doi.org/10.1093/molbev/msab259

Kapun M, Nunez JCB, Bogaerts-Márquez M, Murga-Moreno J, Paris M, Outten J, Coronado-Zamora M, Tern C, Rota-Stabelli O, Guerreiro MPG, Casillas S, Orengo DJ, Puerma E, Kankare M, Ometto L, Loeschcke V, Onder BS, Abbott JK, Schaeffer SW, Rajpurohit S, Behrman EL, Schou MF, Merritt TJS, Lazzaro BP, Glaser-Schmitt A, Argyridou E, Staubach F, Wang Y, Tauber E, Serga SV, Fabian DK, Dyer KA, Wheat CW, Parsch J, Grath S, Savić Veselinović M, Stamenković-Radak M, Jelić M, Buendía-Ruíz AJ, Gómez-Julián MJ, Espinosa-Jimenez ML, Gallardo-Jiménez FD, Patenković A, Erić K, Tanasković M, Ullastres A, Guio L, Merenciano M, Guirao-Rico S, Horváth V, Obbard DJ, Pasyukova E, Alatortsev VE, Vieira CP, Vieira J, Torres JR, Kozeretska I, Maistrenko OM, Montchamp-Moreau C, Mukha DV, Machado HE, Lamb K, Paulo T, Yusuf L, Barbadilla A, Petrov D, Schmidt P, Gonzalez J, Flatt T, Bergland AO, Nielsen R. Drosophila Evolution over Space and Time (DEST): A New Population Genomics Resource. in Molecular Biology and Evolution. 2021;38(12):5782-5805.
doi:10.1093/molbev/msab259 .

Kapun, Martin, Nunez, Joaquin C B, Bogaerts-Márquez, María, Murga-Moreno, Jesús, Paris, Margot, Outten, Joseph, Coronado-Zamora, Marta, Tern, Courtney, Rota-Stabelli, Omar, Guerreiro, Maria P García, Casillas, Sònia, Orengo, Dorcas J, Puerma, Eva, Kankare, Maaria, Ometto, Lino, Loeschcke, Volker, Onder, Banu S, Abbott, Jessica K, Schaeffer, Stephen W, Rajpurohit, Subhash, Behrman, Emily L, Schou, Mads F, Merritt, Thomas J S, Lazzaro, Brian P, Glaser-Schmitt, Amanda, Argyridou, Eliza, Staubach, Fabian, Wang, Yun, Tauber, Eran, Serga, Svitlana V, Fabian, Daniel K, Dyer, Kelly A, Wheat, Christopher W, Parsch, John, Grath, Sonja, Savić Veselinović, Marija, Stamenković-Radak, Marina, Jelić, Mihailo, Buendía-Ruíz, Antonio J, Gómez-Julián, Maria Josefa, Espinosa-Jimenez, Maria Luisa, Gallardo-Jiménez, Francisco D, Patenković, Aleksandra, Erić, Katarina, Tanasković, Marija, Ullastres, Anna, Guio, Lain, Merenciano, Miriam, Guirao-Rico, Sara, Horváth, Vivien, Obbard, Darren J, Pasyukova, Elena, Alatortsev, Vladimir E, Vieira, Cristina P, Vieira, Jorge, Torres, Jorge Roberto, Kozeretska, Iryna, Maistrenko, Oleksandr M, Montchamp-Moreau, Catherine, Mukha, Dmitry V, Machado, Heather E, Lamb, Keric, Paulo, Tânia, Yusuf, Leeban, Barbadilla, Antonio, Petrov, Dmitri, Schmidt, Paul, Gonzalez, Josefa, Flatt, Thomas, Bergland, Alan O, Nielsen, Rasmus, "Drosophila Evolution over Space and Time (DEST): A New Population Genomics Resource" in Molecular Biology and Evolution, 38, no. 12 (2021):5782-5805,
https://doi.org/10.1093/molbev/msab259 . .

TY  - CONF
AU  - Erić, Katarina
AU  - Erić, Pavle
AU  - Davidović, Slobodan
AU  - Savić Veselinović, Marija
AU  - Patenković, Aleksandra
AU  - Stamenković-Radak, Marina
AU  - Tanasković, Marija
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4924
AB  - Climate change and global warming are affecting many insect species in different ways.
Organisms develop diverse mechanisms responding to variable environmental conditions. Due to
global warming and the rise in mean and extreme temperatures, the importance of an individual’s
ability to adapt to temperature stress will further increase. Our focus in this study is the thermal
response, which is considered to be one of the crucial elements of individual fitness and survival in
a fast changing environment. We investigated stress resistance traits: desiccation and heat knock-
down time in two Drospohila subobscura populations from two different altitudes (1080m and 1580m
a.s.l.) along Stara planina mountain slopes in Serbia. The F1 progeny of the mass populations from
both localities was used to establish six experimental groups at three different temperature regimes:
25⁰C, 19⁰C and 16⁰C. Our aim was to determine whether there is a correlation between
altitude/origin of populations and/or laboratory thermal evolution within these resistance traits.
Flies from all groups were tested (scored every hour) for desiccation mortality at those temperatures.
To score heat knock-down time all groups were placed into vials with moistened cotton plugs to
prevent desiccation, kept at 37⁰C for seven hours, and knock down flies were scored every 30
minutes. Our results indicate that the population origin, as well as laboratory thermal evolution
have a significant influence on the analysed traits. Individuals originating from the higher altitudes
reared at higher temperatures show better resistance to thermal shock.
PB  - Basel: MDPI
C3  - Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online
T1  - Thermal Adaptation and Stress Resistance in D. subobscura Populations from Two Altitudes at Stara Planina Mountain (Serbia)
DO  - 10.3390/IECE-10532
SP  - 10532
ER  - 

@conference{
author = "Erić, Katarina and Erić, Pavle and Davidović, Slobodan and Savić Veselinović, Marija and Patenković, Aleksandra and Stamenković-Radak, Marina and Tanasković, Marija",
year = "2021",
abstract = "Climate change and global warming are affecting many insect species in different ways.
Organisms develop diverse mechanisms responding to variable environmental conditions. Due to
global warming and the rise in mean and extreme temperatures, the importance of an individual’s
ability to adapt to temperature stress will further increase. Our focus in this study is the thermal
response, which is considered to be one of the crucial elements of individual fitness and survival in
a fast changing environment. We investigated stress resistance traits: desiccation and heat knock-
down time in two Drospohila subobscura populations from two different altitudes (1080m and 1580m
a.s.l.) along Stara planina mountain slopes in Serbia. The F1 progeny of the mass populations from
both localities was used to establish six experimental groups at three different temperature regimes:
25⁰C, 19⁰C and 16⁰C. Our aim was to determine whether there is a correlation between
altitude/origin of populations and/or laboratory thermal evolution within these resistance traits.
Flies from all groups were tested (scored every hour) for desiccation mortality at those temperatures.
To score heat knock-down time all groups were placed into vials with moistened cotton plugs to
prevent desiccation, kept at 37⁰C for seven hours, and knock down flies were scored every 30
minutes. Our results indicate that the population origin, as well as laboratory thermal evolution
have a significant influence on the analysed traits. Individuals originating from the higher altitudes
reared at higher temperatures show better resistance to thermal shock.",
publisher = "Basel: MDPI",
journal = "Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online",
title = "Thermal Adaptation and Stress Resistance in D. subobscura Populations from Two Altitudes at Stara Planina Mountain (Serbia)",
doi = "10.3390/IECE-10532",
pages = "10532"
}

Erić, K., Erić, P., Davidović, S., Savić Veselinović, M., Patenković, A., Stamenković-Radak, M.,& Tanasković, M.. (2021). Thermal Adaptation and Stress Resistance in D. subobscura Populations from Two Altitudes at Stara Planina Mountain (Serbia). in Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online
Basel: MDPI., 10532.
https://doi.org/10.3390/IECE-10532

Erić K, Erić P, Davidović S, Savić Veselinović M, Patenković A, Stamenković-Radak M, Tanasković M. Thermal Adaptation and Stress Resistance in D. subobscura Populations from Two Altitudes at Stara Planina Mountain (Serbia). in Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online. 2021;:10532.
doi:10.3390/IECE-10532 .

Erić, Katarina, Erić, Pavle, Davidović, Slobodan, Savić Veselinović, Marija, Patenković, Aleksandra, Stamenković-Radak, Marina, Tanasković, Marija, "Thermal Adaptation and Stress Resistance in D. subobscura Populations from Two Altitudes at Stara Planina Mountain (Serbia)" in Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online (2021):10532,
https://doi.org/10.3390/IECE-10532 . .

TY  - CONF
AU  - Rakić, Mina
AU  - Jelić, Mihailo
AU  - Stamenković-Radak, Marina
AU  - Patenković, Aleksandra
AU  - Tanasković, Marija
AU  - Erić, Katarina
AU  - Davidović, Slobodan
AU  - Erić, Pavle
AU  - Savić Veselinović, Marija
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4923
AB  - Heavy metals are one of the most persistent pollutants that are introduced into the environment through human activity and ever so growing industrialization, exploitation and utilization of natural heavy metal deposits. Due to their toxicity, heavy metals have severely negative effects on organisms, mainly through oxidative stress. The objective of this research was to investigate the influence of heavy metals on the expression of six metallothionein genes (Mtn A, B, C, D, E, F), with the intention to show to which extent the concentration of metals and the duration of the exposure influence Mtn gene expression, as well as the potential correlation of certain metallothioneins to a specific metal or combination of them. Larvae of the fruit fly Drosophila subobscura were subjected to the treatments with four heavy metals: zinc, copper, cadmium or lead. A combination of cadmium and lead was also included, to detect the combinatory effect of these metals. Treatment with each of the heavy metals was conducted in two concentrations, in short-term (24 hours) and long-term (10 days) time frame. Gene expression was analyzed with qPCR. The pattern of gene expression in response to various treatments indicates that some genes show specificity to certain metals, while others demonstrate a broader response or no response at all.
PB  - Basel: MDPI
C3  - Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online
T1  - Metallothionein Gene Expression in Drosophila subobscura Indicates Their Different Function in Response to Heavy Metal Exposures
DO  - 10.3390/IECE-10508
SP  - 10508
ER  - 

@conference{
author = "Rakić, Mina and Jelić, Mihailo and Stamenković-Radak, Marina and Patenković, Aleksandra and Tanasković, Marija and Erić, Katarina and Davidović, Slobodan and Erić, Pavle and Savić Veselinović, Marija",
year = "2021",
abstract = "Heavy metals are one of the most persistent pollutants that are introduced into the environment through human activity and ever so growing industrialization, exploitation and utilization of natural heavy metal deposits. Due to their toxicity, heavy metals have severely negative effects on organisms, mainly through oxidative stress. The objective of this research was to investigate the influence of heavy metals on the expression of six metallothionein genes (Mtn A, B, C, D, E, F), with the intention to show to which extent the concentration of metals and the duration of the exposure influence Mtn gene expression, as well as the potential correlation of certain metallothioneins to a specific metal or combination of them. Larvae of the fruit fly Drosophila subobscura were subjected to the treatments with four heavy metals: zinc, copper, cadmium or lead. A combination of cadmium and lead was also included, to detect the combinatory effect of these metals. Treatment with each of the heavy metals was conducted in two concentrations, in short-term (24 hours) and long-term (10 days) time frame. Gene expression was analyzed with qPCR. The pattern of gene expression in response to various treatments indicates that some genes show specificity to certain metals, while others demonstrate a broader response or no response at all.",
publisher = "Basel: MDPI",
journal = "Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online",
title = "Metallothionein Gene Expression in Drosophila subobscura Indicates Their Different Function in Response to Heavy Metal Exposures",
doi = "10.3390/IECE-10508",
pages = "10508"
}

Rakić, M., Jelić, M., Stamenković-Radak, M., Patenković, A., Tanasković, M., Erić, K., Davidović, S., Erić, P.,& Savić Veselinović, M.. (2021). Metallothionein Gene Expression in Drosophila subobscura Indicates Their Different Function in Response to Heavy Metal Exposures. in Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online
Basel: MDPI., 10508.
https://doi.org/10.3390/IECE-10508

Rakić M, Jelić M, Stamenković-Radak M, Patenković A, Tanasković M, Erić K, Davidović S, Erić P, Savić Veselinović M. Metallothionein Gene Expression in Drosophila subobscura Indicates Their Different Function in Response to Heavy Metal Exposures. in Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online. 2021;:10508.
doi:10.3390/IECE-10508 .

Rakić, Mina, Jelić, Mihailo, Stamenković-Radak, Marina, Patenković, Aleksandra, Tanasković, Marija, Erić, Katarina, Davidović, Slobodan, Erić, Pavle, Savić Veselinović, Marija, "Metallothionein Gene Expression in Drosophila subobscura Indicates Their Different Function in Response to Heavy Metal Exposures" in Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online (2021):10508,
https://doi.org/10.3390/IECE-10508 . .

TY  - CONF
AU  - Erić, Pavle
AU  - Patenković, Aleksandra
AU  - Erić, Katarina
AU  - Tanasković, Marija
AU  - Davidović, Slobodan
AU  - Rakić, Mina
AU  - Savić Veselinović, Marija
AU  - Stamenković-Radak, Marina
AU  - Jelić, Mihailo
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4894
AB  - Drosophila obscura is a very common fruit fly inhabiting European forests. This species has
a large number of mitochondrial haplotypes of Cyt b gene. We used experimental lines of D. obscura
to test the adaptive significance of intra -population variability of the mitochondrial genome
(mtDNA) and selective forces that maintain it. We chose three isofemale lines with distinct mitochondrial haplotypes of Cyt b gene from each of the two populations sampled in Serbia. Using backcrossing, we created nine experimental lines for each population with all combinations of mtDNA
haplotypes and nuclear genetic backgrounds (nuDNA). Individuals of both sexes were tested separately for desiccation resistance at two temperatures. Cox proportional hazards model, with four
factors: mtDNA, nuDNA, sex and temperature was used to analyze the survival data. In some comparisons we noticed significant effect of mtDNA on desiccation resistance, while all of them showed
significant effect of interaction between mitochondrial and nuclear genome. Temperature in interaction with mtDNA or mito-nuclear genotype more frequently showed significant effect on desiccation resistance compared to sex in interaction with mtDNA or mito-nuclear genotype. Our results
show adaptive significance of intra-population variation of mtDNA and importance of interactions
between mitochondrial and nuclear genome on fitness. Temperature specific mito-nuclear interaction rather than sex-specific selection on mito-nuclear genotypes maintains mtDNA variability in
this model species.
PB  - Basel: MDPI
C3  - Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online
T1  - Unraveling the adaptive significance of mitochondrial genome variability of Drosophila obscura
DO  - 10.3390/IECE-10522
SP  - 10522
ER  - 

@conference{
author = "Erić, Pavle and Patenković, Aleksandra and Erić, Katarina and Tanasković, Marija and Davidović, Slobodan and Rakić, Mina and Savić Veselinović, Marija and Stamenković-Radak, Marina and Jelić, Mihailo",
year = "2021",
abstract = "Drosophila obscura is a very common fruit fly inhabiting European forests. This species has
a large number of mitochondrial haplotypes of Cyt b gene. We used experimental lines of D. obscura
to test the adaptive significance of intra -population variability of the mitochondrial genome
(mtDNA) and selective forces that maintain it. We chose three isofemale lines with distinct mitochondrial haplotypes of Cyt b gene from each of the two populations sampled in Serbia. Using backcrossing, we created nine experimental lines for each population with all combinations of mtDNA
haplotypes and nuclear genetic backgrounds (nuDNA). Individuals of both sexes were tested separately for desiccation resistance at two temperatures. Cox proportional hazards model, with four
factors: mtDNA, nuDNA, sex and temperature was used to analyze the survival data. In some comparisons we noticed significant effect of mtDNA on desiccation resistance, while all of them showed
significant effect of interaction between mitochondrial and nuclear genome. Temperature in interaction with mtDNA or mito-nuclear genotype more frequently showed significant effect on desiccation resistance compared to sex in interaction with mtDNA or mito-nuclear genotype. Our results
show adaptive significance of intra-population variation of mtDNA and importance of interactions
between mitochondrial and nuclear genome on fitness. Temperature specific mito-nuclear interaction rather than sex-specific selection on mito-nuclear genotypes maintains mtDNA variability in
this model species.",
publisher = "Basel: MDPI",
journal = "Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online",
title = "Unraveling the adaptive significance of mitochondrial genome variability of Drosophila obscura",
doi = "10.3390/IECE-10522",
pages = "10522"
}

Erić, P., Patenković, A., Erić, K., Tanasković, M., Davidović, S., Rakić, M., Savić Veselinović, M., Stamenković-Radak, M.,& Jelić, M.. (2021). Unraveling the adaptive significance of mitochondrial genome variability of Drosophila obscura. in Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online
Basel: MDPI., 10522.
https://doi.org/10.3390/IECE-10522

Erić P, Patenković A, Erić K, Tanasković M, Davidović S, Rakić M, Savić Veselinović M, Stamenković-Radak M, Jelić M. Unraveling the adaptive significance of mitochondrial genome variability of Drosophila obscura. in Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online. 2021;:10522.
doi:10.3390/IECE-10522 .

Erić, Pavle, Patenković, Aleksandra, Erić, Katarina, Tanasković, Marija, Davidović, Slobodan, Rakić, Mina, Savić Veselinović, Marija, Stamenković-Radak, Marina, Jelić, Mihailo, "Unraveling the adaptive significance of mitochondrial genome variability of Drosophila obscura" in Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online (2021):10522,
https://doi.org/10.3390/IECE-10522 . .

TY  - JOUR
AU  - Davidović, Slobodan
AU  - Jelić, Mihailo
AU  - Marinković, Saša
AU  - Mihajlović, Milica
AU  - Tanasić, Vanja
AU  - Hribšek, Irena
AU  - Sušić, Goran
AU  - Dragićević, Milan
AU  - Stamenković-Radak, Marina
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4016
AB  - The Griffon vulture was once a widespread species across the region of Southeast Europe, but it is now endangered and in some parts is completely extinct. In the Balkan Peninsula the largest Griffon vulture inland population inhabits the territory of Serbia. We present, for the first time, the genetic data of this valuable population that could be a source for future reintroduction programs planned in South‑eastern Europe. To characterize the genetic structure of this population we used microsatellite markers from ten loci. Blood samples were collected from 57 chicks directly in the nests during the ongoing monitoring program. We performed a comparative analysis of the obtained data with the existing data from three native populations from French Pyrenees, Croatia, and Israel. We have assessed the genetic differentiation between different native populations and determined the existence of two genetic clusters that differentiate the populations from the Balkan and Iberian Peninsulas. Furthermore, we analysed whether the recent bottleneck events influenced the genetic structure of the populations studied, and we found that all native populations experienced a recent bottleneck event, and that the population of Israel was the least affected. Nevertheless, the parameters of genetic diversity suggest that all analysed populations have retained a similar level of genetic diversity and that the Griffon vulture population from Serbia exhibits the highest value for private alleles. The results of this study suggest that the Griffon vulture populations of the Balkan Peninsula are genetically differentiated from the populations of the Iberian Peninsula, which is an important information for future reintroduction strategies.
PB  - Nature Research
T2  - Scientific Reports
T1  - Genetic diversity of the Griffon vulture population in Serbia and its importance for conservation efforts in the Balkans
IS  - 1
VL  - 10
DO  - 10.1038/s41598-020-77342-1
SP  - 20394
ER  - 

@article{
author = "Davidović, Slobodan and Jelić, Mihailo and Marinković, Saša and Mihajlović, Milica and Tanasić, Vanja and Hribšek, Irena and Sušić, Goran and Dragićević, Milan and Stamenković-Radak, Marina",
year = "2020",
abstract = "The Griffon vulture was once a widespread species across the region of Southeast Europe, but it is now endangered and in some parts is completely extinct. In the Balkan Peninsula the largest Griffon vulture inland population inhabits the territory of Serbia. We present, for the first time, the genetic data of this valuable population that could be a source for future reintroduction programs planned in South‑eastern Europe. To characterize the genetic structure of this population we used microsatellite markers from ten loci. Blood samples were collected from 57 chicks directly in the nests during the ongoing monitoring program. We performed a comparative analysis of the obtained data with the existing data from three native populations from French Pyrenees, Croatia, and Israel. We have assessed the genetic differentiation between different native populations and determined the existence of two genetic clusters that differentiate the populations from the Balkan and Iberian Peninsulas. Furthermore, we analysed whether the recent bottleneck events influenced the genetic structure of the populations studied, and we found that all native populations experienced a recent bottleneck event, and that the population of Israel was the least affected. Nevertheless, the parameters of genetic diversity suggest that all analysed populations have retained a similar level of genetic diversity and that the Griffon vulture population from Serbia exhibits the highest value for private alleles. The results of this study suggest that the Griffon vulture populations of the Balkan Peninsula are genetically differentiated from the populations of the Iberian Peninsula, which is an important information for future reintroduction strategies.",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "Genetic diversity of the Griffon vulture population in Serbia and its importance for conservation efforts in the Balkans",
number = "1",
volume = "10",
doi = "10.1038/s41598-020-77342-1",
pages = "20394"
}

Davidović, S., Jelić, M., Marinković, S., Mihajlović, M., Tanasić, V., Hribšek, I., Sušić, G., Dragićević, M.,& Stamenković-Radak, M.. (2020). Genetic diversity of the Griffon vulture population in Serbia and its importance for conservation efforts in the Balkans. in Scientific Reports
Nature Research., 10(1), 20394.
https://doi.org/10.1038/s41598-020-77342-1

Davidović S, Jelić M, Marinković S, Mihajlović M, Tanasić V, Hribšek I, Sušić G, Dragićević M, Stamenković-Radak M. Genetic diversity of the Griffon vulture population in Serbia and its importance for conservation efforts in the Balkans. in Scientific Reports. 2020;10(1):20394.
doi:10.1038/s41598-020-77342-1 .

Davidović, Slobodan, Jelić, Mihailo, Marinković, Saša, Mihajlović, Milica, Tanasić, Vanja, Hribšek, Irena, Sušić, Goran, Dragićević, Milan, Stamenković-Radak, Marina, "Genetic diversity of the Griffon vulture population in Serbia and its importance for conservation efforts in the Balkans" in Scientific Reports, 10, no. 1 (2020):20394,
https://doi.org/10.1038/s41598-020-77342-1 . .

TY  - DATA
AU  - Davidović, Slobodan
AU  - Jelić, Mihailo
AU  - Marinković, Saša
AU  - Mihajlović, Milica
AU  - Tanasić, Vanja
AU  - Hribšek, Irena
AU  - Sušić, Goran
AU  - Dragićević, Milan
AU  - Stamenković-Radak, Marina
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4183
AB  - Table S1. Parameters of genetic diversity calculated per locus per populations including Hardy-Weinberg equilibrium test and Garza Williamson index. Legend: N - number of gene copies, A - number of alleles, Ho – observed heterozygosity, He – expected heterozygosity, H-W - test for Hardy-Weinberg equilibrium, Ae - effective number of alleles, Ar - allelic range, G-W Garza-Williamson index; Table S2. Number of alleles per locus per populations; Table S3. Tables of significant linkage disequilibrium (significance level p=0.0500, corrected using sequential Bonferroni test). Detected linkage disequlibrium is marked with pink colour; Table S1. Effective population sizes estimated for analysed populations. Legend: N - number of individuals in sample, Ne - effective population size; Table S5. Outcomes of AMOVA analysis based on the variability of 10 autosomal loci for different groups of populations.
PB  - Nature Research
T2  - Scientific Reports
T1  - Supplementary tables for the article: Davidović S, Jelić M, Marinković S, Mihajlović M, Tanasić V, Hribšek I, Sušić G, Dragićević M, Stamenković-Radak M. Genetic diversity of the Griffon vulture population in Serbia and its importance for conservation efforts in the Balkans. Sci Rep. 2020;10(1):20394.
IS  - 1
VL  - 10
SP  - 20394
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4183
ER  - 

@misc{
author = "Davidović, Slobodan and Jelić, Mihailo and Marinković, Saša and Mihajlović, Milica and Tanasić, Vanja and Hribšek, Irena and Sušić, Goran and Dragićević, Milan and Stamenković-Radak, Marina",
year = "2020",
abstract = "Table S1. Parameters of genetic diversity calculated per locus per populations including Hardy-Weinberg equilibrium test and Garza Williamson index. Legend: N - number of gene copies, A - number of alleles, Ho – observed heterozygosity, He – expected heterozygosity, H-W - test for Hardy-Weinberg equilibrium, Ae - effective number of alleles, Ar - allelic range, G-W Garza-Williamson index; Table S2. Number of alleles per locus per populations; Table S3. Tables of significant linkage disequilibrium (significance level p=0.0500, corrected using sequential Bonferroni test). Detected linkage disequlibrium is marked with pink colour; Table S1. Effective population sizes estimated for analysed populations. Legend: N - number of individuals in sample, Ne - effective population size; Table S5. Outcomes of AMOVA analysis based on the variability of 10 autosomal loci for different groups of populations.",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "Supplementary tables for the article: Davidović S, Jelić M, Marinković S, Mihajlović M, Tanasić V, Hribšek I, Sušić G, Dragićević M, Stamenković-Radak M. Genetic diversity of the Griffon vulture population in Serbia and its importance for conservation efforts in the Balkans. Sci Rep. 2020;10(1):20394.",
number = "1",
volume = "10",
pages = "20394",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4183"
}

Davidović, S., Jelić, M., Marinković, S., Mihajlović, M., Tanasić, V., Hribšek, I., Sušić, G., Dragićević, M.,& Stamenković-Radak, M.. (2020). Supplementary tables for the article: Davidović S, Jelić M, Marinković S, Mihajlović M, Tanasić V, Hribšek I, Sušić G, Dragićević M, Stamenković-Radak M. Genetic diversity of the Griffon vulture population in Serbia and its importance for conservation efforts in the Balkans. Sci Rep. 2020;10(1):20394.. in Scientific Reports
Nature Research., 10(1), 20394.
https://hdl.handle.net/21.15107/rcub_ibiss_4183

Davidović S, Jelić M, Marinković S, Mihajlović M, Tanasić V, Hribšek I, Sušić G, Dragićević M, Stamenković-Radak M. Supplementary tables for the article: Davidović S, Jelić M, Marinković S, Mihajlović M, Tanasić V, Hribšek I, Sušić G, Dragićević M, Stamenković-Radak M. Genetic diversity of the Griffon vulture population in Serbia and its importance for conservation efforts in the Balkans. Sci Rep. 2020;10(1):20394.. in Scientific Reports. 2020;10(1):20394.
https://hdl.handle.net/21.15107/rcub_ibiss_4183 .

Davidović, Slobodan, Jelić, Mihailo, Marinković, Saša, Mihajlović, Milica, Tanasić, Vanja, Hribšek, Irena, Sušić, Goran, Dragićević, Milan, Stamenković-Radak, Marina, "Supplementary tables for the article: Davidović S, Jelić M, Marinković S, Mihajlović M, Tanasić V, Hribšek I, Sušić G, Dragićević M, Stamenković-Radak M. Genetic diversity of the Griffon vulture population in Serbia and its importance for conservation efforts in the Balkans. Sci Rep. 2020;10(1):20394." in Scientific Reports, 10, no. 1 (2020):20394,
https://hdl.handle.net/21.15107/rcub_ibiss_4183 .

TY  - DATA
AU  - Davidović, Slobodan
AU  - Jelić, Mihailo
AU  - Marinković, Saša
AU  - Mihajlović, Milica
AU  - Tanasić, Vanja
AU  - Hribšek, Irena
AU  - Sušić, Goran
AU  - Dragićević, Milan
AU  - Stamenković-Radak, Marina
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4017
AB  - Figure  S1Matrix  of the average number of pairwise differences and Nei's distances detected in native  and  introduced  G.  fulvus  populations  based  on  the  analysis  of  microsatellite  loci.  The  average  number  of  pairwise  differences  between  populations  is  presented  above  diagonal,  the  average  number  of  pairwise  differences  within  the  population  is  presented  diagonal  and  Nei's  distances  are  presented  below  diagonal.  The  average  number  of  pairwise  differences  are  presented in Table 1.; Figure S2 Matrix of pairwise FSTdistances between the G. fulvus population of Serbia and other native  and  introduced  G.  fulvus  populations  based  on  the  analysis  of  10  microsatellite  loci.Statist ically significant FST values are marked with an asterisk (*). Population pairwise FSTvalues are presented in Table 3.; Figure S3 Ln values of probability for the assumed number of genetic clusters. Figure S4   (a) Proportions of inferred STRUCTURE clusters (K=2) from the native and introduced populations.(b) Proportions of the inferred STRUCTURE clusters (K=2) from the individuals.
PB  - Nature Research
T2  - Scientific Reports
T1  - Supplementary figures for the article: Davidović S, Jelić M, Marinković S, Mihajlović M, Tanasić V, Hribšek I, Sušić G, Dragićević M, Stamenković-Radak M. Genetic diversity of the Griffon vulture population in Serbia and its importance for conservation efforts in the Balkans. Sci Rep. 2020;10(1):20394.
IS  - 1
VL  - 10
SP  - 20394
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4017
ER  - 

@misc{
author = "Davidović, Slobodan and Jelić, Mihailo and Marinković, Saša and Mihajlović, Milica and Tanasić, Vanja and Hribšek, Irena and Sušić, Goran and Dragićević, Milan and Stamenković-Radak, Marina",
year = "2020",
abstract = "Figure  S1Matrix  of the average number of pairwise differences and Nei's distances detected in native  and  introduced  G.  fulvus  populations  based  on  the  analysis  of  microsatellite  loci.  The  average  number  of  pairwise  differences  between  populations  is  presented  above  diagonal,  the  average  number  of  pairwise  differences  within  the  population  is  presented  diagonal  and  Nei's  distances  are  presented  below  diagonal.  The  average  number  of  pairwise  differences  are  presented in Table 1.; Figure S2 Matrix of pairwise FSTdistances between the G. fulvus population of Serbia and other native  and  introduced  G.  fulvus  populations  based  on  the  analysis  of  10  microsatellite  loci.Statist ically significant FST values are marked with an asterisk (*). Population pairwise FSTvalues are presented in Table 3.; Figure S3 Ln values of probability for the assumed number of genetic clusters. Figure S4   (a) Proportions of inferred STRUCTURE clusters (K=2) from the native and introduced populations.(b) Proportions of the inferred STRUCTURE clusters (K=2) from the individuals.",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "Supplementary figures for the article: Davidović S, Jelić M, Marinković S, Mihajlović M, Tanasić V, Hribšek I, Sušić G, Dragićević M, Stamenković-Radak M. Genetic diversity of the Griffon vulture population in Serbia and its importance for conservation efforts in the Balkans. Sci Rep. 2020;10(1):20394.",
number = "1",
volume = "10",
pages = "20394",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4017"
}

Davidović, S., Jelić, M., Marinković, S., Mihajlović, M., Tanasić, V., Hribšek, I., Sušić, G., Dragićević, M.,& Stamenković-Radak, M.. (2020). Supplementary figures for the article: Davidović S, Jelić M, Marinković S, Mihajlović M, Tanasić V, Hribšek I, Sušić G, Dragićević M, Stamenković-Radak M. Genetic diversity of the Griffon vulture population in Serbia and its importance for conservation efforts in the Balkans. Sci Rep. 2020;10(1):20394.. in Scientific Reports
Nature Research., 10(1), 20394.
https://hdl.handle.net/21.15107/rcub_ibiss_4017

Davidović S, Jelić M, Marinković S, Mihajlović M, Tanasić V, Hribšek I, Sušić G, Dragićević M, Stamenković-Radak M. Supplementary figures for the article: Davidović S, Jelić M, Marinković S, Mihajlović M, Tanasić V, Hribšek I, Sušić G, Dragićević M, Stamenković-Radak M. Genetic diversity of the Griffon vulture population in Serbia and its importance for conservation efforts in the Balkans. Sci Rep. 2020;10(1):20394.. in Scientific Reports. 2020;10(1):20394.
https://hdl.handle.net/21.15107/rcub_ibiss_4017 .

Davidović, Slobodan, Jelić, Mihailo, Marinković, Saša, Mihajlović, Milica, Tanasić, Vanja, Hribšek, Irena, Sušić, Goran, Dragićević, Milan, Stamenković-Radak, Marina, "Supplementary figures for the article: Davidović S, Jelić M, Marinković S, Mihajlović M, Tanasić V, Hribšek I, Sušić G, Dragićević M, Stamenković-Radak M. Genetic diversity of the Griffon vulture population in Serbia and its importance for conservation efforts in the Balkans. Sci Rep. 2020;10(1):20394." in Scientific Reports, 10, no. 1 (2020):20394,
https://hdl.handle.net/21.15107/rcub_ibiss_4017 .

TY  - JOUR
AU  - Savić Veselinović, Marija
AU  - Kurbalija Novičić, Zorana
AU  - Kenig, Bojan
AU  - Jelić, Mihailo
AU  - Patenković, Aleksandra
AU  - Tanasković, Marija
AU  - Pertoldi, Cino
AU  - Stamenković-Radak, Marina
AU  - Anđelković, Marko
PY  - 2019
UR  - http://www.eje.cz/doi/10.14411/eje.2019.051.html
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3669
AB  - To explore local adaptation in wild populations at a fine spatial scale we characterized the genetic variability of eight closely located populations of Drosophila subobscura and its associations with microhabitat environmental conditions. Three different genetic markers were assessed: chromosomal inversions, a SNP of mitochondrial ND5 gene and nuclear microsatellites. Population genetic analyses of chromosomal variability revealed significant genetic differentiation between these populations. Gene arrangement frequencies on the E chromosome contributed most to these differences. We also investigated role of mito-nuclear epistasis in mitochondrial genome differentiation and revealed weak linkage disequilibrium (LD) exclusively between O3+4 inversion arrangement and mitochondrial DNA haplotype I in two populations. In addition, the trend in the LD between OST chromosomal arrangement and haplotype II was general in the total sample. Microsatellite analysis revealed an absence of stochastic processes, like census reduction, upon population differentiation. Only a small amount of the genetic variation is related to geographic distance, while most (97%) is attributable to other factors and in some degree to microhabitat variables (temperature, humidity). The analysis of these factors revealed they effect inversion arrangement frequencies, especially E1+2+9, EST and OST. Even though this model organism is known for its high mobility and mostly large effective population size, the results presented here reveal that local adaptations can occur even at a small spatial scale. We propose that locally adapted alleles within chromosomal inversions, as well as joint selective pressures acting on mitochondrial and nuclear genomes, are responsible for the observed adaptation to microhabitat conditions.
PB  - Biology Centre, AS CR
T2  - European Journal of Entomology
T1  - Local adaptation at fine spatial scale through chromosomal inversions and mito-nuclear epistasis: Findings in Drosophila subobscura (Diptera: Drosophilidae)
IS  - 1
VL  - 116
DO  - 10.14411/eje.2019.051
SP  - 492
EP  - 503
ER  - 

@article{
author = "Savić Veselinović, Marija and Kurbalija Novičić, Zorana and Kenig, Bojan and Jelić, Mihailo and Patenković, Aleksandra and Tanasković, Marija and Pertoldi, Cino and Stamenković-Radak, Marina and Anđelković, Marko",
year = "2019",
abstract = "To explore local adaptation in wild populations at a fine spatial scale we characterized the genetic variability of eight closely located populations of Drosophila subobscura and its associations with microhabitat environmental conditions. Three different genetic markers were assessed: chromosomal inversions, a SNP of mitochondrial ND5 gene and nuclear microsatellites. Population genetic analyses of chromosomal variability revealed significant genetic differentiation between these populations. Gene arrangement frequencies on the E chromosome contributed most to these differences. We also investigated role of mito-nuclear epistasis in mitochondrial genome differentiation and revealed weak linkage disequilibrium (LD) exclusively between O3+4 inversion arrangement and mitochondrial DNA haplotype I in two populations. In addition, the trend in the LD between OST chromosomal arrangement and haplotype II was general in the total sample. Microsatellite analysis revealed an absence of stochastic processes, like census reduction, upon population differentiation. Only a small amount of the genetic variation is related to geographic distance, while most (97%) is attributable to other factors and in some degree to microhabitat variables (temperature, humidity). The analysis of these factors revealed they effect inversion arrangement frequencies, especially E1+2+9, EST and OST. Even though this model organism is known for its high mobility and mostly large effective population size, the results presented here reveal that local adaptations can occur even at a small spatial scale. We propose that locally adapted alleles within chromosomal inversions, as well as joint selective pressures acting on mitochondrial and nuclear genomes, are responsible for the observed adaptation to microhabitat conditions.",
publisher = "Biology Centre, AS CR",
journal = "European Journal of Entomology",
title = "Local adaptation at fine spatial scale through chromosomal inversions and mito-nuclear epistasis: Findings in Drosophila subobscura (Diptera: Drosophilidae)",
number = "1",
volume = "116",
doi = "10.14411/eje.2019.051",
pages = "492-503"
}

Savić Veselinović, M., Kurbalija Novičić, Z., Kenig, B., Jelić, M., Patenković, A., Tanasković, M., Pertoldi, C., Stamenković-Radak, M.,& Anđelković, M.. (2019). Local adaptation at fine spatial scale through chromosomal inversions and mito-nuclear epistasis: Findings in Drosophila subobscura (Diptera: Drosophilidae). in European Journal of Entomology
Biology Centre, AS CR., 116(1), 492-503.
https://doi.org/10.14411/eje.2019.051

Savić Veselinović M, Kurbalija Novičić Z, Kenig B, Jelić M, Patenković A, Tanasković M, Pertoldi C, Stamenković-Radak M, Anđelković M. Local adaptation at fine spatial scale through chromosomal inversions and mito-nuclear epistasis: Findings in Drosophila subobscura (Diptera: Drosophilidae). in European Journal of Entomology. 2019;116(1):492-503.
doi:10.14411/eje.2019.051 .

Savić Veselinović, Marija, Kurbalija Novičić, Zorana, Kenig, Bojan, Jelić, Mihailo, Patenković, Aleksandra, Tanasković, Marija, Pertoldi, Cino, Stamenković-Radak, Marina, Anđelković, Marko, "Local adaptation at fine spatial scale through chromosomal inversions and mito-nuclear epistasis: Findings in Drosophila subobscura (Diptera: Drosophilidae)" in European Journal of Entomology, 116, no. 1 (2019):492-503,
https://doi.org/10.14411/eje.2019.051 . .

TY  - JOUR
AU  - Stamenković-Radak, Marina
AU  - Kenig, Bojan
AU  - Đurakić, M
AU  - Jelić, M
AU  - Eric, K
AU  - Anđelković, Marko
PY  - 2019
UR  - https://www.int-res.com/abstracts/cr/v77/n3/p205-217/
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3360
AB  - Ecological and evolutionary factors, together with abiotic conditions, affect biogeographic patterns of genetic entities. The spatial and temporal variability of chromosomal inversions of Drosophila subobscura suggests that this species can serve as a good model for studying the effects of environmental change on the genetic structure of natural populations. A comprehensive meta-analysis of the association of environmental and climatic variables with inversion diversity patterns was performed on 20 D. subobscura populations from the central part of the Balkan Peninsula. Environmental data consisted of 3 sets of variables related to temperature and precipitation, extracted from 2 climatic databases, averaged over a 3 month period, and using biological instead of calendar dates of sampling. Arrangement frequency patterns are likely driven by a synergistic effect of factors related to temperature and precipitation. The frequencies of standard chromosomal arrangements tend to co-vary positively with precipitation, whereas parameters related to temperature appear to favor higher frequencies of the inverted and more complex chromosomal arrangements. A complex relationship among local environmental variables is evident from the results and reflects the probable effect of an altitudinal shift; the altitudinal gradient of inversions is different from their latitudinal gradient. The pattern of inversions is generally not associated with environmental variables, and a particular inversion cannot be a predictive genetic marker of global climate change. Populations in different habitats are subjected to habitat-specific selection regimes, while demographic factors and population history also affect the genetic variability pattern observed.
T2  - Climate Research
T2  - Climate Research
T1  - Associations between environmental variability and inversion polymorphism of Drosophila subobscura: meta-analysis of populations from the Central Balkans
IS  - 3
VL  - 77
DO  - 10.3354/cr01551
SP  - 205
EP  - 217
ER  - 

@article{
author = "Stamenković-Radak, Marina and Kenig, Bojan and Đurakić, M and Jelić, M and Eric, K and Anđelković, Marko",
year = "2019",
abstract = "Ecological and evolutionary factors, together with abiotic conditions, affect biogeographic patterns of genetic entities. The spatial and temporal variability of chromosomal inversions of Drosophila subobscura suggests that this species can serve as a good model for studying the effects of environmental change on the genetic structure of natural populations. A comprehensive meta-analysis of the association of environmental and climatic variables with inversion diversity patterns was performed on 20 D. subobscura populations from the central part of the Balkan Peninsula. Environmental data consisted of 3 sets of variables related to temperature and precipitation, extracted from 2 climatic databases, averaged over a 3 month period, and using biological instead of calendar dates of sampling. Arrangement frequency patterns are likely driven by a synergistic effect of factors related to temperature and precipitation. The frequencies of standard chromosomal arrangements tend to co-vary positively with precipitation, whereas parameters related to temperature appear to favor higher frequencies of the inverted and more complex chromosomal arrangements. A complex relationship among local environmental variables is evident from the results and reflects the probable effect of an altitudinal shift; the altitudinal gradient of inversions is different from their latitudinal gradient. The pattern of inversions is generally not associated with environmental variables, and a particular inversion cannot be a predictive genetic marker of global climate change. Populations in different habitats are subjected to habitat-specific selection regimes, while demographic factors and population history also affect the genetic variability pattern observed.",
journal = "Climate Research, Climate Research",
title = "Associations between environmental variability and inversion polymorphism of Drosophila subobscura: meta-analysis of populations from the Central Balkans",
number = "3",
volume = "77",
doi = "10.3354/cr01551",
pages = "205-217"
}

Stamenković-Radak, M., Kenig, B., Đurakić, M., Jelić, M., Eric, K.,& Anđelković, M.. (2019). Associations between environmental variability and inversion polymorphism of Drosophila subobscura: meta-analysis of populations from the Central Balkans. in Climate Research, 77(3), 205-217.
https://doi.org/10.3354/cr01551

Stamenković-Radak M, Kenig B, Đurakić M, Jelić M, Eric K, Anđelković M. Associations between environmental variability and inversion polymorphism of Drosophila subobscura: meta-analysis of populations from the Central Balkans. in Climate Research. 2019;77(3):205-217.
doi:10.3354/cr01551 .

Stamenković-Radak, Marina, Kenig, Bojan, Đurakić, M, Jelić, M, Eric, K, Anđelković, Marko, "Associations between environmental variability and inversion polymorphism of Drosophila subobscura: meta-analysis of populations from the Central Balkans" in Climate Research, 77, no. 3 (2019):205-217,
https://doi.org/10.3354/cr01551 . .

TY  - JOUR
AU  - Erić, Pavle
AU  - Jelić, Mihailo
AU  - Savić-Veselinović, Marija
AU  - Kenig, Bojan
AU  - Anđelković, Marko
AU  - Stamenković-Radak, Marina
PY  - 2019
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0534-00121901213E
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3369
AB  - Mitochondrial DNA variability of Drosophila subobscura Collin from Southeastern Serbia was studied with respect to Restriction Site Analysis (RSA) of complete mitochondrial genome and the nucleotide sequence of Cytochrome b (Cyt b) gene. The aim was to shed more light on the evolutionary forces that shape mtDNA variation of this species. Samples were collected from two sites in the foothills of the Balkan Mountains. No genetic differentiation was found between groups and most of the variation was observed within them. Restriction analysis revealed two main haplotypes and several rare ones. The sequencing of Cyt b gene showed larger number of haplotypes, among which, one is being the most common. The majority of singletons differed from the most frequent haplotype by one nucleotide change. Although some of the observed nucleotide differences may affect their host’s fitness, the observed pattern of variation is consistent with the seasonal fluctuations in population size.
T2  - Genetika
T2  - Genetika
T1  - Nucleotide diversity of Cyt b gene in drosophila subobscura Collin
IS  - 1
VL  - 51
DO  - 10.2298/GENSR1901213E
SP  - 213
EP  - 226
ER  - 

@article{
author = "Erić, Pavle and Jelić, Mihailo and Savić-Veselinović, Marija and Kenig, Bojan and Anđelković, Marko and Stamenković-Radak, Marina",
year = "2019",
abstract = "Mitochondrial DNA variability of Drosophila subobscura Collin from Southeastern Serbia was studied with respect to Restriction Site Analysis (RSA) of complete mitochondrial genome and the nucleotide sequence of Cytochrome b (Cyt b) gene. The aim was to shed more light on the evolutionary forces that shape mtDNA variation of this species. Samples were collected from two sites in the foothills of the Balkan Mountains. No genetic differentiation was found between groups and most of the variation was observed within them. Restriction analysis revealed two main haplotypes and several rare ones. The sequencing of Cyt b gene showed larger number of haplotypes, among which, one is being the most common. The majority of singletons differed from the most frequent haplotype by one nucleotide change. Although some of the observed nucleotide differences may affect their host’s fitness, the observed pattern of variation is consistent with the seasonal fluctuations in population size.",
journal = "Genetika, Genetika",
title = "Nucleotide diversity of Cyt b gene in drosophila subobscura Collin",
number = "1",
volume = "51",
doi = "10.2298/GENSR1901213E",
pages = "213-226"
}

Erić, P., Jelić, M., Savić-Veselinović, M., Kenig, B., Anđelković, M.,& Stamenković-Radak, M.. (2019). Nucleotide diversity of Cyt b gene in drosophila subobscura Collin. in Genetika, 51(1), 213-226.
https://doi.org/10.2298/GENSR1901213E

Erić P, Jelić M, Savić-Veselinović M, Kenig B, Anđelković M, Stamenković-Radak M. Nucleotide diversity of Cyt b gene in drosophila subobscura Collin. in Genetika. 2019;51(1):213-226.
doi:10.2298/GENSR1901213E .

Erić, Pavle, Jelić, Mihailo, Savić-Veselinović, Marija, Kenig, Bojan, Anđelković, Marko, Stamenković-Radak, Marina, "Nucleotide diversity of Cyt b gene in drosophila subobscura Collin" in Genetika, 51, no. 1 (2019):213-226,
https://doi.org/10.2298/GENSR1901213E . .

TY  - JOUR
AU  - Savić Veselinović, Marija
AU  - Pavković-Lučić, Sofija
AU  - Kurbalija Novičić, Zorana
AU  - Jelić, Mihailo
AU  - Stamenković-Radak, Marina
AU  - Anđelković, Marko
PY  - 2017
UR  - http://doi.wiley.com/10.1111/1744-7917.12257
UR  - http://www.ncbi.nlm.nih.gov/pubmed/26235310
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2918
AB  - According to current theoretical predictions, any deleterious mutations that reduce nonsexual fitness may have a negative influence on mating success. This means that sexual selection may remove deleterious mutations from the populations. Males of good genetic quality should be more successful in mating, compared to the males of lower genetic quality. As mating success is a condition dependent trait, large fractions of the genome may be a target of sexual selection and many behavioral traits are likely to be condition dependent. We manipulated the genetic quality of Drosophila subobscura males by inducing mutations with ionizing radiation and observed the effects of the obtained heterozygous mutations on male mating behavior: courtship occurrence, courtship latency, mating occurrence, latency to mating and duration of mating. We found possible effects of mutations. Females mated more frequently with male progeny of nonirradiated males and that these males courted females faster compared to the male progeny of irradiated males. Our findings indicate a possible important role of sexual selection in purging deleterious mutations.
PB  - Blackwell Publishing Ltd
T2  - Insect Science
T2  - Insect science
T1  - Mating behavior as an indicator of quality of Drosophila subobscura males?
IS  - 1
VL  - 24
DO  - 10.1111/1744-7917.12257
SP  - 122
EP  - 132
ER  - 

@article{
author = "Savić Veselinović, Marija and Pavković-Lučić, Sofija and Kurbalija Novičić, Zorana and Jelić, Mihailo and Stamenković-Radak, Marina and Anđelković, Marko",
year = "2017",
abstract = "According to current theoretical predictions, any deleterious mutations that reduce nonsexual fitness may have a negative influence on mating success. This means that sexual selection may remove deleterious mutations from the populations. Males of good genetic quality should be more successful in mating, compared to the males of lower genetic quality. As mating success is a condition dependent trait, large fractions of the genome may be a target of sexual selection and many behavioral traits are likely to be condition dependent. We manipulated the genetic quality of Drosophila subobscura males by inducing mutations with ionizing radiation and observed the effects of the obtained heterozygous mutations on male mating behavior: courtship occurrence, courtship latency, mating occurrence, latency to mating and duration of mating. We found possible effects of mutations. Females mated more frequently with male progeny of nonirradiated males and that these males courted females faster compared to the male progeny of irradiated males. Our findings indicate a possible important role of sexual selection in purging deleterious mutations.",
publisher = "Blackwell Publishing Ltd",
journal = "Insect Science, Insect science",
title = "Mating behavior as an indicator of quality of Drosophila subobscura males?",
number = "1",
volume = "24",
doi = "10.1111/1744-7917.12257",
pages = "122-132"
}

Savić Veselinović, M., Pavković-Lučić, S., Kurbalija Novičić, Z., Jelić, M., Stamenković-Radak, M.,& Anđelković, M.. (2017). Mating behavior as an indicator of quality of Drosophila subobscura males?. in Insect Science
Blackwell Publishing Ltd., 24(1), 122-132.
https://doi.org/10.1111/1744-7917.12257

Savić Veselinović M, Pavković-Lučić S, Kurbalija Novičić Z, Jelić M, Stamenković-Radak M, Anđelković M. Mating behavior as an indicator of quality of Drosophila subobscura males?. in Insect Science. 2017;24(1):122-132.
doi:10.1111/1744-7917.12257 .

Savić Veselinović, Marija, Pavković-Lučić, Sofija, Kurbalija Novičić, Zorana, Jelić, Mihailo, Stamenković-Radak, Marina, Anđelković, Marko, "Mating behavior as an indicator of quality of Drosophila subobscura males?" in Insect Science, 24, no. 1 (2017):122-132,
https://doi.org/10.1111/1744-7917.12257 . .