TY  - JOUR
AU  - Dmitrović, Slavica
AU  - Simonović, Ana
AU  - Banjac, Nevena
AU  - Savic, Jelena
AU  - Cingel, Aleksandar
AU  - Filipović, Biljana
AU  - Ninković, Slavica
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2085
AB  - The effects of Chenopodium murale root exudates, applied as phytotoxic
   medias (PMs), were tested on Arabidopsis thaliana and Triticum aestivum.
   The effects of PMs, where wild-type roots (K), hairy roots derived from
   roots (R clones) or from cotyledons (C clones) were cultured, were
   different. K medium suppressed Arabidopsis germination, while other PMs
   reduced root and leaf elongation and the number of rosette leaves. R
   media were more phytotoxic than C media. Treatment of Arabidopsis with
   R8 down-regulated expression of core cell cycle genes: cyclin-dependent
   kinase (CDK) A1;1, four B-class CDKs, and cyclins CYCA3;1, CYCB2;4,
   CYCD4;2 and CYCH1 in root and shoot tips. Only CYCD2;1 transcript was
   elevated in treated shoots, but down-regulated in roots. Wheat Ta-CDC2
   and Ta-CYCD2 genes showed the same expression profiles as their
   Arabidopsis counterparts, CDKA1;1 and CYCD2;1. PMs also caused increase
   of antioxidative enzyme activities in both plants. Exposure of
   Arabidopsis to PMs induced one catalase isoform, but repressed another,
   resulting in no net change of catalase activity. Wheat seedlings treated
   with PMs had catalase activity significantly elevated in all treatments,
   particularly in shoots. In both plants, PMs induced the activity of
   different peroxidase isozymes and total peroxidase activity. Both plants
   responded to phytotoxic treatments by induction of CuZn-superoxide
   dismutase. Thus, the phytotoxicity of C. murale root exudates is, at
   least partially, based on down-regulation of the cell cycle regulators
   and on generation of oxidative stress in the affected plants. We propose
   that C. murale root exudates should be considered as means of biological
   weed control.
T2  - Plant Growth Regulation
T1  - Hairy root exudates of allelopathic weed Chenopodium murale L. induce
 oxidative stress and down-regulate core cell cycle genes in Arabidopsis
 and wheat seedlings
IS  - 1
VL  - 75
DO  - 10.1007/s10725-014-9959-z
SP  - 365
EP  - 382
ER  - 

@article{
author = "Dmitrović, Slavica and Simonović, Ana and Banjac, Nevena and Savic, Jelena and Cingel, Aleksandar and Filipović, Biljana and Ninković, Slavica",
year = "2015",
abstract = "The effects of Chenopodium murale root exudates, applied as phytotoxic
   medias (PMs), were tested on Arabidopsis thaliana and Triticum aestivum.
   The effects of PMs, where wild-type roots (K), hairy roots derived from
   roots (R clones) or from cotyledons (C clones) were cultured, were
   different. K medium suppressed Arabidopsis germination, while other PMs
   reduced root and leaf elongation and the number of rosette leaves. R
   media were more phytotoxic than C media. Treatment of Arabidopsis with
   R8 down-regulated expression of core cell cycle genes: cyclin-dependent
   kinase (CDK) A1;1, four B-class CDKs, and cyclins CYCA3;1, CYCB2;4,
   CYCD4;2 and CYCH1 in root and shoot tips. Only CYCD2;1 transcript was
   elevated in treated shoots, but down-regulated in roots. Wheat Ta-CDC2
   and Ta-CYCD2 genes showed the same expression profiles as their
   Arabidopsis counterparts, CDKA1;1 and CYCD2;1. PMs also caused increase
   of antioxidative enzyme activities in both plants. Exposure of
   Arabidopsis to PMs induced one catalase isoform, but repressed another,
   resulting in no net change of catalase activity. Wheat seedlings treated
   with PMs had catalase activity significantly elevated in all treatments,
   particularly in shoots. In both plants, PMs induced the activity of
   different peroxidase isozymes and total peroxidase activity. Both plants
   responded to phytotoxic treatments by induction of CuZn-superoxide
   dismutase. Thus, the phytotoxicity of C. murale root exudates is, at
   least partially, based on down-regulation of the cell cycle regulators
   and on generation of oxidative stress in the affected plants. We propose
   that C. murale root exudates should be considered as means of biological
   weed control.",
journal = "Plant Growth Regulation",
title = "Hairy root exudates of allelopathic weed Chenopodium murale L. induce
 oxidative stress and down-regulate core cell cycle genes in Arabidopsis
 and wheat seedlings",
number = "1",
volume = "75",
doi = "10.1007/s10725-014-9959-z",
pages = "365-382"
}

Dmitrović, S., Simonović, A., Banjac, N., Savic, J., Cingel, A., Filipović, B.,& Ninković, S.. (2015). Hairy root exudates of allelopathic weed Chenopodium murale L. induce
 oxidative stress and down-regulate core cell cycle genes in Arabidopsis
 and wheat seedlings. in Plant Growth Regulation, 75(1), 365-382.
https://doi.org/10.1007/s10725-014-9959-z

Dmitrović S, Simonović A, Banjac N, Savic J, Cingel A, Filipović B, Ninković S. Hairy root exudates of allelopathic weed Chenopodium murale L. induce
 oxidative stress and down-regulate core cell cycle genes in Arabidopsis
 and wheat seedlings. in Plant Growth Regulation. 2015;75(1):365-382.
doi:10.1007/s10725-014-9959-z .

Dmitrović, Slavica, Simonović, Ana, Banjac, Nevena, Savic, Jelena, Cingel, Aleksandar, Filipović, Biljana, Ninković, Slavica, "Hairy root exudates of allelopathic weed Chenopodium murale L. induce
 oxidative stress and down-regulate core cell cycle genes in Arabidopsis
 and wheat seedlings" in Plant Growth Regulation, 75, no. 1 (2015):365-382,
https://doi.org/10.1007/s10725-014-9959-z . .

TY  - JOUR
AU  - Cingel, Aleksandar
AU  - Savic, Jelena
AU  - Ćosić, Tatjana
AU  - Raspor, Martin
AU  - Ghalawenji, Nabil
AU  - Smigocki, Ann
AU  - Ninković, Slavica
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2032
AB  - The evaluation of transgenic plants which is usually carried out under
   controlled conditions in culture rooms and greenhouses can yield
   valuable information about the influence of introduced genes on a
   transgenic plant phenotype. However, an overall assessment of plant
   performance can only be made by testing transgenic plants in the field
   environment. Thus, the effects of pyramided rice cystatin genes OCI and
   OCII on morphological parameters of transgenic potato cv. Desiree,
   Dragacevka and Jelica lines were compared under in vitro, greenhouse,
   and field conditions. All analyzed OC co-expressing transgenic lines
   exhibited normal phenotype, both in vitro and in greenhouse conditions.
   In the field environment, eight of nine OCI/OCII lines were similar to
   the wild-type control plants in their general phenotypic appearance.
   Yield parameters, such as tuber number and tuber weight for these
   phenotypically normal OCI/OCII lines, were also comparable to the
   controls. Only transgenic cv. Jelica line 4 plants exhibited slightly
   reduced growth, atypical leaf morphology and, contrary to the plants of
   other transgenic lines and untransformed controls, failed to flower.
   However, despite the phenotypic and developmental changes under field
   conditions, the OCI/OCII Jelica line 4 did not exhibit a significant
   decrease in tuber yield. Stacking of OCI and OCII genes preserves
   important attributes of the parental lines, confirming that this
   approach could be suitable for improving agronomical traits in potato.
T2  - Archives of Biological Sciences
T1  - Phenotypic performance of transgenic potato (Solanum tuberosum L.) plants with pyramided rice cystatin genes (OCI and OCII)
IS  - 3
VL  - 67
DO  - 10.2298/ABS141201058C
SP  - 957
EP  - 964
ER  - 

@article{
author = "Cingel, Aleksandar and Savic, Jelena and Ćosić, Tatjana and Raspor, Martin and Ghalawenji, Nabil and Smigocki, Ann and Ninković, Slavica",
year = "2015",
abstract = "The evaluation of transgenic plants which is usually carried out under
   controlled conditions in culture rooms and greenhouses can yield
   valuable information about the influence of introduced genes on a
   transgenic plant phenotype. However, an overall assessment of plant
   performance can only be made by testing transgenic plants in the field
   environment. Thus, the effects of pyramided rice cystatin genes OCI and
   OCII on morphological parameters of transgenic potato cv. Desiree,
   Dragacevka and Jelica lines were compared under in vitro, greenhouse,
   and field conditions. All analyzed OC co-expressing transgenic lines
   exhibited normal phenotype, both in vitro and in greenhouse conditions.
   In the field environment, eight of nine OCI/OCII lines were similar to
   the wild-type control plants in their general phenotypic appearance.
   Yield parameters, such as tuber number and tuber weight for these
   phenotypically normal OCI/OCII lines, were also comparable to the
   controls. Only transgenic cv. Jelica line 4 plants exhibited slightly
   reduced growth, atypical leaf morphology and, contrary to the plants of
   other transgenic lines and untransformed controls, failed to flower.
   However, despite the phenotypic and developmental changes under field
   conditions, the OCI/OCII Jelica line 4 did not exhibit a significant
   decrease in tuber yield. Stacking of OCI and OCII genes preserves
   important attributes of the parental lines, confirming that this
   approach could be suitable for improving agronomical traits in potato.",
journal = "Archives of Biological Sciences",
title = "Phenotypic performance of transgenic potato (Solanum tuberosum L.) plants with pyramided rice cystatin genes (OCI and OCII)",
number = "3",
volume = "67",
doi = "10.2298/ABS141201058C",
pages = "957-964"
}

Cingel, A., Savic, J., Ćosić, T., Raspor, M., Ghalawenji, N., Smigocki, A.,& Ninković, S.. (2015). Phenotypic performance of transgenic potato (Solanum tuberosum L.) plants with pyramided rice cystatin genes (OCI and OCII). in Archives of Biological Sciences, 67(3), 957-964.
https://doi.org/10.2298/ABS141201058C

Cingel A, Savic J, Ćosić T, Raspor M, Ghalawenji N, Smigocki A, Ninković S. Phenotypic performance of transgenic potato (Solanum tuberosum L.) plants with pyramided rice cystatin genes (OCI and OCII). in Archives of Biological Sciences. 2015;67(3):957-964.
doi:10.2298/ABS141201058C .

Cingel, Aleksandar, Savic, Jelena, Ćosić, Tatjana, Raspor, Martin, Ghalawenji, Nabil, Smigocki, Ann, Ninković, Slavica, "Phenotypic performance of transgenic potato (Solanum tuberosum L.) plants with pyramided rice cystatin genes (OCI and OCII)" in Archives of Biological Sciences, 67, no. 3 (2015):957-964,
https://doi.org/10.2298/ABS141201058C . .

TY  - JOUR
AU  - Ćosić, Tatjana
AU  - Motyka, Vaclav
AU  - Raspor, Martin
AU  - Savic, Jelena
AU  - Cingel, Aleksandar
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan
AU  - Travnickova, Alena
AU  - Dobrev, Petre I.
AU  - Bohanec, Borut
AU  - Ninković, Slavica
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1956
AB  - Kohlrabi (Brassica oleracea var. gongylodes) cultivars Vienna Purple
   (VP) and Vienna White (VW) were tested for their ability of de novo
   organogenesis in vitro. Root, cotyledon, hypocotyl explants and intact
   seedlings were cultivated on Murashige and Skoog (MS) media supplemented
   with different cytokinins: benzyladenine (BA), thidiazuron (TDZ), trans-
   or cis-zeatin. All tested cytokinins, including cis-zeatin, induced
   shoot regeneration from hypocotyl explants and intact seedlings, with
   seedlings being most successful for regeneration efficiency and
   viability of regenerated shoots in both cultivars. The highest frequency
   of shoot regeneration was achieved on MS with BA (60 \%) or TDZ (50 \%)
   for VP; and with BA (50 \%), TDZ (47.5 \%) or transZ (37.5 \%) for VW.
   Measurements of the endogenous cytokinin and indole-3-acetic acid (IAA)
   contents in both hypocotyl explants and seedlings with regenerated
   shoots (HRSs and SRSs) suggested that the observed differences in
   organogenic response between these two types of explants were related to
   their cytokinin and IAA contents. HRSs generally exhibited elevated
   amounts of total cytokinins, while SRSs displayed a higher IAA/bioactive
   cytokinins ratio. Shoots regenerated from seedlings were further
   successfully multiplicated on a medium supplemented with BA (0.5 mg
   L-1). The rooting potential of multiplicated shoots was tested on media
   supplemented with 2 or 4 mg L-1 indole-3-butyric acid (IBA), with the
   higher concentration of IBA leading to more efficient rooting. Rooted
   plantlets were successfully planted into soil and flow cytometric
   analysis did not reveal ploidy variations, indicating that the described
   protocol is fast and efficient for kohlrabi regeneration.
T2  - Plant Cell Tissue and Organ Culture
T1  - In vitro shoot organogenesis and comparative analysis of endogenous
 phytohormones in kohlrabi (Brassica oleracea var. gongylodes): effects
 of genotype, explant type and applied cytokinins
IS  - 3
VL  - 121
DO  - 10.1007/s11240-015-0742-2
SP  - 741
EP  - 760
ER  - 

@article{
author = "Ćosić, Tatjana and Motyka, Vaclav and Raspor, Martin and Savic, Jelena and Cingel, Aleksandar and Vinterhalter, Branka and Vinterhalter, Dragan and Travnickova, Alena and Dobrev, Petre I. and Bohanec, Borut and Ninković, Slavica",
year = "2015",
abstract = "Kohlrabi (Brassica oleracea var. gongylodes) cultivars Vienna Purple
   (VP) and Vienna White (VW) were tested for their ability of de novo
   organogenesis in vitro. Root, cotyledon, hypocotyl explants and intact
   seedlings were cultivated on Murashige and Skoog (MS) media supplemented
   with different cytokinins: benzyladenine (BA), thidiazuron (TDZ), trans-
   or cis-zeatin. All tested cytokinins, including cis-zeatin, induced
   shoot regeneration from hypocotyl explants and intact seedlings, with
   seedlings being most successful for regeneration efficiency and
   viability of regenerated shoots in both cultivars. The highest frequency
   of shoot regeneration was achieved on MS with BA (60 \%) or TDZ (50 \%)
   for VP; and with BA (50 \%), TDZ (47.5 \%) or transZ (37.5 \%) for VW.
   Measurements of the endogenous cytokinin and indole-3-acetic acid (IAA)
   contents in both hypocotyl explants and seedlings with regenerated
   shoots (HRSs and SRSs) suggested that the observed differences in
   organogenic response between these two types of explants were related to
   their cytokinin and IAA contents. HRSs generally exhibited elevated
   amounts of total cytokinins, while SRSs displayed a higher IAA/bioactive
   cytokinins ratio. Shoots regenerated from seedlings were further
   successfully multiplicated on a medium supplemented with BA (0.5 mg
   L-1). The rooting potential of multiplicated shoots was tested on media
   supplemented with 2 or 4 mg L-1 indole-3-butyric acid (IBA), with the
   higher concentration of IBA leading to more efficient rooting. Rooted
   plantlets were successfully planted into soil and flow cytometric
   analysis did not reveal ploidy variations, indicating that the described
   protocol is fast and efficient for kohlrabi regeneration.",
journal = "Plant Cell Tissue and Organ Culture",
title = "In vitro shoot organogenesis and comparative analysis of endogenous
 phytohormones in kohlrabi (Brassica oleracea var. gongylodes): effects
 of genotype, explant type and applied cytokinins",
number = "3",
volume = "121",
doi = "10.1007/s11240-015-0742-2",
pages = "741-760"
}

Ćosić, T., Motyka, V., Raspor, M., Savic, J., Cingel, A., Vinterhalter, B., Vinterhalter, D., Travnickova, A., Dobrev, P. I., Bohanec, B.,& Ninković, S.. (2015). In vitro shoot organogenesis and comparative analysis of endogenous
 phytohormones in kohlrabi (Brassica oleracea var. gongylodes): effects
 of genotype, explant type and applied cytokinins. in Plant Cell Tissue and Organ Culture, 121(3), 741-760.
https://doi.org/10.1007/s11240-015-0742-2

Ćosić T, Motyka V, Raspor M, Savic J, Cingel A, Vinterhalter B, Vinterhalter D, Travnickova A, Dobrev PI, Bohanec B, Ninković S. In vitro shoot organogenesis and comparative analysis of endogenous
 phytohormones in kohlrabi (Brassica oleracea var. gongylodes): effects
 of genotype, explant type and applied cytokinins. in Plant Cell Tissue and Organ Culture. 2015;121(3):741-760.
doi:10.1007/s11240-015-0742-2 .

Ćosić, Tatjana, Motyka, Vaclav, Raspor, Martin, Savic, Jelena, Cingel, Aleksandar, Vinterhalter, Branka, Vinterhalter, Dragan, Travnickova, Alena, Dobrev, Petre I., Bohanec, Borut, Ninković, Slavica, "In vitro shoot organogenesis and comparative analysis of endogenous
 phytohormones in kohlrabi (Brassica oleracea var. gongylodes): effects
 of genotype, explant type and applied cytokinins" in Plant Cell Tissue and Organ Culture, 121, no. 3 (2015):741-760,
https://doi.org/10.1007/s11240-015-0742-2 . .

TY  - JOUR
AU  - Cingel, Aleksandar
AU  - Savic, Jelena
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan
AU  - Kostic, Miroslav
AU  - Šešlija Jovanović, Darka
AU  - Smigocki, Ann
AU  - Ninković, Slavica
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1923
AB  - Plant proteinase inhibitors (PIs) are attractive tools for crop
   improvement and their heterologous expression can enhance insect
   resistance in transgenic plants. PI oryzacystatin II (OCII), isolated
   from rice, showed potential in controlling pests that utilize cysteine
   proteinases for protein digestion. To evaluate the applicability of the
   OCII gene in enhancing plant defence, OCII-transformed potatoes were
   bioassayed for resistance to Colorado potato beetle (Leptinotarsa
   decemlineata Say). Feeding on transformed leaves of potato cultivars
   Desiree and Jelica significantly affected larval growth and development,
   but did not change mortality rates. During the L2 and L3 developmental
   stages larvae consumed the OCII-transformed foliage faster as compared
   to the nontransformed control. Also these larvae reached the prepupal
   stage (end of L4 stage) 2 days earlier than those fed on control leaves.
   However, the total amounts of consumed OCII-transformed leaves were up
   to 23 \% lower than of control, and the maximal weights of prepupal
   larvae were reduced by up to 18 \% as compared to larvae fed on
   nontransformed leaves. The reduction in insect fitness reported in this
   study in combination with other control measures, could lead to improved
   CPB resistance management in potato.
T2  - Transgenic Research
T1  - Growth and development of Colorado potato beetle larvae, Leptinotarsa
 decemlineata, on potato plants expressing the oryzacystatin II
 proteinase inhibitor
IS  - 4
VL  - 24
DO  - 10.1007/s11248-015-9873-9
SP  - 729
EP  - 740
ER  - 

@article{
author = "Cingel, Aleksandar and Savic, Jelena and Vinterhalter, Branka and Vinterhalter, Dragan and Kostic, Miroslav and Šešlija Jovanović, Darka and Smigocki, Ann and Ninković, Slavica",
year = "2015",
abstract = "Plant proteinase inhibitors (PIs) are attractive tools for crop
   improvement and their heterologous expression can enhance insect
   resistance in transgenic plants. PI oryzacystatin II (OCII), isolated
   from rice, showed potential in controlling pests that utilize cysteine
   proteinases for protein digestion. To evaluate the applicability of the
   OCII gene in enhancing plant defence, OCII-transformed potatoes were
   bioassayed for resistance to Colorado potato beetle (Leptinotarsa
   decemlineata Say). Feeding on transformed leaves of potato cultivars
   Desiree and Jelica significantly affected larval growth and development,
   but did not change mortality rates. During the L2 and L3 developmental
   stages larvae consumed the OCII-transformed foliage faster as compared
   to the nontransformed control. Also these larvae reached the prepupal
   stage (end of L4 stage) 2 days earlier than those fed on control leaves.
   However, the total amounts of consumed OCII-transformed leaves were up
   to 23 \% lower than of control, and the maximal weights of prepupal
   larvae were reduced by up to 18 \% as compared to larvae fed on
   nontransformed leaves. The reduction in insect fitness reported in this
   study in combination with other control measures, could lead to improved
   CPB resistance management in potato.",
journal = "Transgenic Research",
title = "Growth and development of Colorado potato beetle larvae, Leptinotarsa
 decemlineata, on potato plants expressing the oryzacystatin II
 proteinase inhibitor",
number = "4",
volume = "24",
doi = "10.1007/s11248-015-9873-9",
pages = "729-740"
}

Cingel, A., Savic, J., Vinterhalter, B., Vinterhalter, D., Kostic, M., Šešlija Jovanović, D., Smigocki, A.,& Ninković, S.. (2015). Growth and development of Colorado potato beetle larvae, Leptinotarsa
 decemlineata, on potato plants expressing the oryzacystatin II
 proteinase inhibitor. in Transgenic Research, 24(4), 729-740.
https://doi.org/10.1007/s11248-015-9873-9

Cingel A, Savic J, Vinterhalter B, Vinterhalter D, Kostic M, Šešlija Jovanović D, Smigocki A, Ninković S. Growth and development of Colorado potato beetle larvae, Leptinotarsa
 decemlineata, on potato plants expressing the oryzacystatin II
 proteinase inhibitor. in Transgenic Research. 2015;24(4):729-740.
doi:10.1007/s11248-015-9873-9 .

Cingel, Aleksandar, Savic, Jelena, Vinterhalter, Branka, Vinterhalter, Dragan, Kostic, Miroslav, Šešlija Jovanović, Darka, Smigocki, Ann, Ninković, Slavica, "Growth and development of Colorado potato beetle larvae, Leptinotarsa
 decemlineata, on potato plants expressing the oryzacystatin II
 proteinase inhibitor" in Transgenic Research, 24, no. 4 (2015):729-740,
https://doi.org/10.1007/s11248-015-9873-9 . .

TY  - JOUR
AU  - Momčilović, Ivana
AU  - Pantelic, Danijel
AU  - Hfidan, Masoud
AU  - Savic, Jelena
AU  - Vinterhalter, Dragan
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2180
AB  - Superoxide dismutase (SOD) in-gel activity assay with selective
   inhibitors (KCN and H2O2) is one of the most commonly used methods for
   identification of SOD isoform types, i.e., FeSOD, MnSOD or Cu/ZnSOD, and
   evaluation of oxidative stress response in plants. However, there are
   potential pitfalls that surround this assay, such as problem to detect
   isoforms with low activity, comigration of SOD isoforms or application
   of inappropriate inhibitor concentration. We propose an improved method
   based on the combination of in-gel analysis of SOD activity and
   native-PAGE immunoblotting for identification of isoforms and
   determination of SOD isoenzyme activity pattern in potato. Depending on
   cultivar and growing conditions, one MnSOD, 3 FeSOD and 5-6 Cu/ZnSOD
   isoforms were identified in potato leaves. The most important
   qualitative difference between ex vitro- and in vitro-grown plants was
   the presence of additional FeSOD and Cu/ZnSOD isoforms in plantlets
   grown in vitro. Compared with results of in-gel activity assay with
   selective inhibitors, new method allowed accurate identification of
   comigrating FeSOD and Cu/ZnSOD isoforms and two protein bands of
   ambiguous identities. Potato SODs were also characterized by SDS-PAGE
   immunoblotting and single MnSOD (23.6 kDa), three Cu/ZnSOD polypeptides
   (17.9, 17 and 16.3 kDa) and single FeSOD (25.1 kDa) polypeptide were
   detected in leaves of four examined cultivars. The difference in the
   number of FeSOD and Cu/ZnSOD isoforms/polypeptides between native-PAGE
   and SDS-PAGE immunoblots suggests that SOD proteins may have undergone
   post-translational modifications affecting protein mobility or existence
   of isoforms that differ from each other in total protein charge, but not
   in molecular weight.
T2  - Acta Physiologiae Plantarum
T1  - Improved procedure for detection of superoxide dismutase isoforms in
 potato, Solanum tuberosum L.
IS  - 8
VL  - 36
DO  - 10.1007/s11738-014-1583-z
SP  - 2059
EP  - 2066
ER  - 

@article{
author = "Momčilović, Ivana and Pantelic, Danijel and Hfidan, Masoud and Savic, Jelena and Vinterhalter, Dragan",
year = "2014",
abstract = "Superoxide dismutase (SOD) in-gel activity assay with selective
   inhibitors (KCN and H2O2) is one of the most commonly used methods for
   identification of SOD isoform types, i.e., FeSOD, MnSOD or Cu/ZnSOD, and
   evaluation of oxidative stress response in plants. However, there are
   potential pitfalls that surround this assay, such as problem to detect
   isoforms with low activity, comigration of SOD isoforms or application
   of inappropriate inhibitor concentration. We propose an improved method
   based on the combination of in-gel analysis of SOD activity and
   native-PAGE immunoblotting for identification of isoforms and
   determination of SOD isoenzyme activity pattern in potato. Depending on
   cultivar and growing conditions, one MnSOD, 3 FeSOD and 5-6 Cu/ZnSOD
   isoforms were identified in potato leaves. The most important
   qualitative difference between ex vitro- and in vitro-grown plants was
   the presence of additional FeSOD and Cu/ZnSOD isoforms in plantlets
   grown in vitro. Compared with results of in-gel activity assay with
   selective inhibitors, new method allowed accurate identification of
   comigrating FeSOD and Cu/ZnSOD isoforms and two protein bands of
   ambiguous identities. Potato SODs were also characterized by SDS-PAGE
   immunoblotting and single MnSOD (23.6 kDa), three Cu/ZnSOD polypeptides
   (17.9, 17 and 16.3 kDa) and single FeSOD (25.1 kDa) polypeptide were
   detected in leaves of four examined cultivars. The difference in the
   number of FeSOD and Cu/ZnSOD isoforms/polypeptides between native-PAGE
   and SDS-PAGE immunoblots suggests that SOD proteins may have undergone
   post-translational modifications affecting protein mobility or existence
   of isoforms that differ from each other in total protein charge, but not
   in molecular weight.",
journal = "Acta Physiologiae Plantarum",
title = "Improved procedure for detection of superoxide dismutase isoforms in
 potato, Solanum tuberosum L.",
number = "8",
volume = "36",
doi = "10.1007/s11738-014-1583-z",
pages = "2059-2066"
}

Momčilović, I., Pantelic, D., Hfidan, M., Savic, J.,& Vinterhalter, D.. (2014). Improved procedure for detection of superoxide dismutase isoforms in
 potato, Solanum tuberosum L.. in Acta Physiologiae Plantarum, 36(8), 2059-2066.
https://doi.org/10.1007/s11738-014-1583-z

Momčilović I, Pantelic D, Hfidan M, Savic J, Vinterhalter D. Improved procedure for detection of superoxide dismutase isoforms in
 potato, Solanum tuberosum L.. in Acta Physiologiae Plantarum. 2014;36(8):2059-2066.
doi:10.1007/s11738-014-1583-z .

Momčilović, Ivana, Pantelic, Danijel, Hfidan, Masoud, Savic, Jelena, Vinterhalter, Dragan, "Improved procedure for detection of superoxide dismutase isoforms in
 potato, Solanum tuberosum L." in Acta Physiologiae Plantarum, 36, no. 8 (2014):2059-2066,
https://doi.org/10.1007/s11738-014-1583-z . .