TY  - JOUR
AU  - Kostić, Marina
AU  - Ivanov, Marija
AU  - Fernandes, Ângela
AU  - Calhelha, Ricardo C.
AU  - Glamočlija, Jasmina
AU  - Barros, Lillian
AU  - Soković, Marina
AU  - Ćirić, Ana
PY  - 2023
UR  - https://www.mdpi.com/2309-608X/9/1/70
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9864295
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5461
AB  - Mushrooms are valued worldwide for their nutritional, organoleptic and chemical properties. The aim of this study was to determine the chemical composition (free sugars, organic acids, fatty acids, tocopherols and phenolic compounds) and bioactivity of three wild mushrooms (Lactarius piperatus, Lactarius quietus and Lactarius vellereus) from Serbia. Chemical analysis was performed with HPLC-RI and UFLC-PDA (for hydrophilic compounds) and with GC-FID and HPLC-FP (for lipophilic compounds). The analysis of phenolic compounds was performed by UFLC-DAD. Biological activities were evaluated using three different assays (microdilution, TBARS and SRB assays). The results showed that the fruiting bodies were rich in mannitol and trehalose. The main organic acids were oxalic acid and citric acid. As for lipophilic components, stearic, oleic and linoleic acids and β-tocopherol dominated in all the species studied. In addition, the methanolic and ethanolic extracts obtained showed antibacterial, antibiofilm and antioxidant properties. As for cytotoxicity, the extracts were not toxic or only moderately toxic toward different tumor cell lines. According to the results, the selected Serbian mushrooms are a rich source of bioactive compounds, and due to their good biological potential, they can be further exploited as functional ingredients beneficial to human health (antimicrobial agents, antioxidants).
PB  - Basel: MDPI
T2  - Journal of Fungi
T1  - A Comparative Study of Lactarius Mushrooms: Chemical Characterization, Antibacterial, Antibiofilm, Antioxidant and Cytotoxic Activity
IS  - 1
VL  - 9
DO  - 10.3390/jof9010070
SP  - 70
ER  - 

@article{
author = "Kostić, Marina and Ivanov, Marija and Fernandes, Ângela and Calhelha, Ricardo C. and Glamočlija, Jasmina and Barros, Lillian and Soković, Marina and Ćirić, Ana",
year = "2023",
abstract = "Mushrooms are valued worldwide for their nutritional, organoleptic and chemical properties. The aim of this study was to determine the chemical composition (free sugars, organic acids, fatty acids, tocopherols and phenolic compounds) and bioactivity of three wild mushrooms (Lactarius piperatus, Lactarius quietus and Lactarius vellereus) from Serbia. Chemical analysis was performed with HPLC-RI and UFLC-PDA (for hydrophilic compounds) and with GC-FID and HPLC-FP (for lipophilic compounds). The analysis of phenolic compounds was performed by UFLC-DAD. Biological activities were evaluated using three different assays (microdilution, TBARS and SRB assays). The results showed that the fruiting bodies were rich in mannitol and trehalose. The main organic acids were oxalic acid and citric acid. As for lipophilic components, stearic, oleic and linoleic acids and β-tocopherol dominated in all the species studied. In addition, the methanolic and ethanolic extracts obtained showed antibacterial, antibiofilm and antioxidant properties. As for cytotoxicity, the extracts were not toxic or only moderately toxic toward different tumor cell lines. According to the results, the selected Serbian mushrooms are a rich source of bioactive compounds, and due to their good biological potential, they can be further exploited as functional ingredients beneficial to human health (antimicrobial agents, antioxidants).",
publisher = "Basel: MDPI",
journal = "Journal of Fungi",
title = "A Comparative Study of Lactarius Mushrooms: Chemical Characterization, Antibacterial, Antibiofilm, Antioxidant and Cytotoxic Activity",
number = "1",
volume = "9",
doi = "10.3390/jof9010070",
pages = "70"
}

Kostić, M., Ivanov, M., Fernandes, Â., Calhelha, R. C., Glamočlija, J., Barros, L., Soković, M.,& Ćirić, A.. (2023). A Comparative Study of Lactarius Mushrooms: Chemical Characterization, Antibacterial, Antibiofilm, Antioxidant and Cytotoxic Activity. in Journal of Fungi
Basel: MDPI., 9(1), 70.
https://doi.org/10.3390/jof9010070

Kostić M, Ivanov M, Fernandes Â, Calhelha RC, Glamočlija J, Barros L, Soković M, Ćirić A. A Comparative Study of Lactarius Mushrooms: Chemical Characterization, Antibacterial, Antibiofilm, Antioxidant and Cytotoxic Activity. in Journal of Fungi. 2023;9(1):70.
doi:10.3390/jof9010070 .

Kostić, Marina, Ivanov, Marija, Fernandes, Ângela, Calhelha, Ricardo C., Glamočlija, Jasmina, Barros, Lillian, Soković, Marina, Ćirić, Ana, "A Comparative Study of Lactarius Mushrooms: Chemical Characterization, Antibacterial, Antibiofilm, Antioxidant and Cytotoxic Activity" in Journal of Fungi, 9, no. 1 (2023):70,
https://doi.org/10.3390/jof9010070 . .

TY  - JOUR
AU  - Cavichi, Lucas Vinicius
AU  - Liberal, Ângela
AU  - Dias, Maria Inês
AU  - Mandim, Filipa
AU  - Pinela, José
AU  - Kostić, Marina
AU  - Soković, Marina
AU  - Kalschne, Daneysa Lahis
AU  - Fernandes, Ângela
AU  - Canan, Cristiane
AU  - Barros, Lillian
AU  - Amaral, Joana S.
PY  - 2023
UR  - https://www.mdpi.com/2304-8158/12/1/192
UR  - http://www.ncbi.nlm.nih.gov/pubmed/36613411
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9818490
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5400
AB  - In recent years, the interest in products of natural origin has boosted the exploitation and use of plants as food and sources of bioactive compounds, especially wild plants widely used in different cultures for several purposes. Commelina erecta is a wild edible plant (WEP) traditionally used as food and medicine, about which few studies exist. Thus, this study aimed at enhancing the knowledge about its nutritional, chemical and bioactive profile, considering different plant parts and development stages, in order to increase its inclusion in the diet of South American communities. The nutritional profile was found to be similar to other WEP frequently consumed in Brazil. Thirteen phenolic compounds (HPLC-DAD-ESI/MS) were tentatively identified, with apigenin, luteolin and quercetin derivatives being the most abundant. Fructose and oxalic acid were the major sugar and organic acid, respectively, in the aerial parts of C. erecta, and four isoforms of tocopherols were also identified. Regarding the plant's antioxidant activity, the EC50 values varied between 18.4 and 1060 µg/mL in the inhibition of lipid peroxidation assay (TBARS) and between 53 and 115 µg/mL in the oxidative haemolysis inhibition (OxHLIA) assay. The hydroethanolic extract obtained from stems at the flowering stage also presented anti-inflammatory activity. In general, all the extracts evidenced promising antimicrobial activity. Altogether, these results reinforce the traditional use of this plant species as food and medicine to support the diet of needier populations and also promote food sovereignty and sustainability.
PB  - Basel: MDPI
T2  - Foods
T1  - Chemical Composition and Biological Activity of Commelina erecta: An Edible Wild Plant Consumed in Brazil
IS  - 1
VL  - 12
DO  - 10.3390/foods12010192
SP  - 192
ER  - 

@article{
author = "Cavichi, Lucas Vinicius and Liberal, Ângela and Dias, Maria Inês and Mandim, Filipa and Pinela, José and Kostić, Marina and Soković, Marina and Kalschne, Daneysa Lahis and Fernandes, Ângela and Canan, Cristiane and Barros, Lillian and Amaral, Joana S.",
year = "2023",
abstract = "In recent years, the interest in products of natural origin has boosted the exploitation and use of plants as food and sources of bioactive compounds, especially wild plants widely used in different cultures for several purposes. Commelina erecta is a wild edible plant (WEP) traditionally used as food and medicine, about which few studies exist. Thus, this study aimed at enhancing the knowledge about its nutritional, chemical and bioactive profile, considering different plant parts and development stages, in order to increase its inclusion in the diet of South American communities. The nutritional profile was found to be similar to other WEP frequently consumed in Brazil. Thirteen phenolic compounds (HPLC-DAD-ESI/MS) were tentatively identified, with apigenin, luteolin and quercetin derivatives being the most abundant. Fructose and oxalic acid were the major sugar and organic acid, respectively, in the aerial parts of C. erecta, and four isoforms of tocopherols were also identified. Regarding the plant's antioxidant activity, the EC50 values varied between 18.4 and 1060 µg/mL in the inhibition of lipid peroxidation assay (TBARS) and between 53 and 115 µg/mL in the oxidative haemolysis inhibition (OxHLIA) assay. The hydroethanolic extract obtained from stems at the flowering stage also presented anti-inflammatory activity. In general, all the extracts evidenced promising antimicrobial activity. Altogether, these results reinforce the traditional use of this plant species as food and medicine to support the diet of needier populations and also promote food sovereignty and sustainability.",
publisher = "Basel: MDPI",
journal = "Foods",
title = "Chemical Composition and Biological Activity of Commelina erecta: An Edible Wild Plant Consumed in Brazil",
number = "1",
volume = "12",
doi = "10.3390/foods12010192",
pages = "192"
}

Cavichi, L. V., Liberal, Â., Dias, M. I., Mandim, F., Pinela, J., Kostić, M., Soković, M., Kalschne, D. L., Fernandes, Â., Canan, C., Barros, L.,& Amaral, J. S.. (2023). Chemical Composition and Biological Activity of Commelina erecta: An Edible Wild Plant Consumed in Brazil. in Foods
Basel: MDPI., 12(1), 192.
https://doi.org/10.3390/foods12010192

Cavichi LV, Liberal Â, Dias MI, Mandim F, Pinela J, Kostić M, Soković M, Kalschne DL, Fernandes Â, Canan C, Barros L, Amaral JS. Chemical Composition and Biological Activity of Commelina erecta: An Edible Wild Plant Consumed in Brazil. in Foods. 2023;12(1):192.
doi:10.3390/foods12010192 .

Cavichi, Lucas Vinicius, Liberal, Ângela, Dias, Maria Inês, Mandim, Filipa, Pinela, José, Kostić, Marina, Soković, Marina, Kalschne, Daneysa Lahis, Fernandes, Ângela, Canan, Cristiane, Barros, Lillian, Amaral, Joana S., "Chemical Composition and Biological Activity of Commelina erecta: An Edible Wild Plant Consumed in Brazil" in Foods, 12, no. 1 (2023):192,
https://doi.org/10.3390/foods12010192 . .

TY  - JOUR
AU  - Petrou, Anthi
AU  - Geronikaki, Athina
AU  - Kartsev, Victor
AU  - Kousaxidis, Antonios
AU  - Papadimitriou-Tsantarliotou, Aliki
AU  - Kostić, Marina
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Nicolaou, Ioannis
AU  - Vizirianakis, Ioannis S.
PY  - 2023
UR  - https://www.mdpi.com/1424-8247/16/1/131
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9865890
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5459
AB  - Herein, we report the experimental evaluation of the antimicrobial activity of seventeen new (Z)-methyl 3-(4-oxo-2-thioxothiazolidin-5-ylidene)methyl)-1H-indole-2-carboxylate derivatives. All tested compounds exhibited antibacterial activity against eight Gram-positive and Gram-negative bacteria. Their activity exceeded those of ampicillin as well as streptomycin by 10-50 fold. The most sensitive bacterium was En. Cloacae, while E. coli was the most resistant one, followed by M. flavus. The most active compound appeared to be compound 8 with MIC at 0.004-0.03 mg/mL and MBC at 0.008-0.06 mg/mL. The antifungal activity of tested compounds was good to excellent with MIC in the range of 0.004-0.06 mg/mL, with compound 15 being the most potent. T. viride was the most sensitive fungal, while A. fumigatus was the most resistant one. Docking studies revealed that the inhibition of E. coli MurB is probably responsible for their antibacterial activity, while 14a-lanosterol demethylase of CYP51Ca is involved in the mechanism of antifungal activity. Furthermore, drug-likeness and ADMET profile prediction were performed. Finally, the cytotoxicity studies were performed for the most active compounds using MTT assay against normal MRC5 cells.
PB  - Basel: MDPI
T2  - Pharmaceuticals
T1  - N-Derivatives of (Z)-Methyl 3-(4-Oxo-2-thioxothiazolidin-5-ylidene)methyl)-1H-indole-2-carboxylates as Antimicrobial Agents-In Silico and In Vitro Evaluation
IS  - 1
VL  - 16
DO  - 10.3390/ph16010131
SP  - 131
ER  - 

@article{
author = "Petrou, Anthi and Geronikaki, Athina and Kartsev, Victor and Kousaxidis, Antonios and Papadimitriou-Tsantarliotou, Aliki and Kostić, Marina and Ivanov, Marija and Soković, Marina and Nicolaou, Ioannis and Vizirianakis, Ioannis S.",
year = "2023",
abstract = "Herein, we report the experimental evaluation of the antimicrobial activity of seventeen new (Z)-methyl 3-(4-oxo-2-thioxothiazolidin-5-ylidene)methyl)-1H-indole-2-carboxylate derivatives. All tested compounds exhibited antibacterial activity against eight Gram-positive and Gram-negative bacteria. Their activity exceeded those of ampicillin as well as streptomycin by 10-50 fold. The most sensitive bacterium was En. Cloacae, while E. coli was the most resistant one, followed by M. flavus. The most active compound appeared to be compound 8 with MIC at 0.004-0.03 mg/mL and MBC at 0.008-0.06 mg/mL. The antifungal activity of tested compounds was good to excellent with MIC in the range of 0.004-0.06 mg/mL, with compound 15 being the most potent. T. viride was the most sensitive fungal, while A. fumigatus was the most resistant one. Docking studies revealed that the inhibition of E. coli MurB is probably responsible for their antibacterial activity, while 14a-lanosterol demethylase of CYP51Ca is involved in the mechanism of antifungal activity. Furthermore, drug-likeness and ADMET profile prediction were performed. Finally, the cytotoxicity studies were performed for the most active compounds using MTT assay against normal MRC5 cells.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals",
title = "N-Derivatives of (Z)-Methyl 3-(4-Oxo-2-thioxothiazolidin-5-ylidene)methyl)-1H-indole-2-carboxylates as Antimicrobial Agents-In Silico and In Vitro Evaluation",
number = "1",
volume = "16",
doi = "10.3390/ph16010131",
pages = "131"
}

Petrou, A., Geronikaki, A., Kartsev, V., Kousaxidis, A., Papadimitriou-Tsantarliotou, A., Kostić, M., Ivanov, M., Soković, M., Nicolaou, I.,& Vizirianakis, I. S.. (2023). N-Derivatives of (Z)-Methyl 3-(4-Oxo-2-thioxothiazolidin-5-ylidene)methyl)-1H-indole-2-carboxylates as Antimicrobial Agents-In Silico and In Vitro Evaluation. in Pharmaceuticals
Basel: MDPI., 16(1), 131.
https://doi.org/10.3390/ph16010131

Petrou A, Geronikaki A, Kartsev V, Kousaxidis A, Papadimitriou-Tsantarliotou A, Kostić M, Ivanov M, Soković M, Nicolaou I, Vizirianakis IS. N-Derivatives of (Z)-Methyl 3-(4-Oxo-2-thioxothiazolidin-5-ylidene)methyl)-1H-indole-2-carboxylates as Antimicrobial Agents-In Silico and In Vitro Evaluation. in Pharmaceuticals. 2023;16(1):131.
doi:10.3390/ph16010131 .

Petrou, Anthi, Geronikaki, Athina, Kartsev, Victor, Kousaxidis, Antonios, Papadimitriou-Tsantarliotou, Aliki, Kostić, Marina, Ivanov, Marija, Soković, Marina, Nicolaou, Ioannis, Vizirianakis, Ioannis S., "N-Derivatives of (Z)-Methyl 3-(4-Oxo-2-thioxothiazolidin-5-ylidene)methyl)-1H-indole-2-carboxylates as Antimicrobial Agents-In Silico and In Vitro Evaluation" in Pharmaceuticals, 16, no. 1 (2023):131,
https://doi.org/10.3390/ph16010131 . .

TY  - JOUR
AU  - Perić, Mirjana
AU  - Čairović, Aleksandra
AU  - Špadijer Gostović, Aleksandra
AU  - Stanimirović, Dragan
AU  - Kostić, Marina
AU  - Ivanov, Marija
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Pavlović, Vladimir B.
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6005
AB  - The purpose of this study was to evaluate the surface texture and biofilm adhe sion of veneered or CAD/CAM milled zirconia (partially stabilized with yttrium) after professional
oral hygiene procedures. The samples (4 × 4 mm, thickness 2 mm; n = 72) were separated from
zirconia blanks (3Y-TZP-LA). One group was veenered with ceramics, and the other group of samples
was CAD/CAM milled. Each group had two subgroups: polished and glazed. The samples were
subjected to simulated strokes of professional brushing using abrasive paste and ultrasonic scaling.
The parameters of surface micromorphology and receptivity to biofilm were calculated before and
after simulating the given methods of the professional maintenance of oral hygiene. Scanning electron
microscopy (SEM) was used to evaluate zirconia surface properties. Microbial (bacterial/fungal)
species (Staphylococcus aureus, Streptococcus sanguinis and Candida albicans) were used and cultured
on respective sterilized zirconia surfaces. Colony-forming unit (CFU) counts were used to quantify
the amount of biofilm formation on zirconia samples surfaces. Results: The SEM analysis showed
the greatest change in surface microtopography after the use of ultrasonic scaling on glazed zirconia
samples. Less formation of colonies on the surfaces of CAD/CAM milled zirconia restorations was
observed. Conclusion: Routine methods of oral hygiene professional maintenance can damage the
surfaces of glazed zirconia restorations.
PB  - Basel: MDPI
T2  - Applied Sciences
T1  - Biofilm Adhesion and Micromorphology Analysis after Professional Oral Hygiene Procedures on CAD/CAM Milled and Veneered Zirconia Restorations: In Vitro Study
IS  - 14
VL  - 13
DO  - 10.3390/app13148389
SP  - 8389
ER  - 

@article{
author = "Perić, Mirjana and Čairović, Aleksandra and Špadijer Gostović, Aleksandra and Stanimirović, Dragan and Kostić, Marina and Ivanov, Marija and Ćirić, Ana and Glamočlija, Jasmina and Pavlović, Vladimir B.",
year = "2023",
abstract = "The purpose of this study was to evaluate the surface texture and biofilm adhe sion of veneered or CAD/CAM milled zirconia (partially stabilized with yttrium) after professional
oral hygiene procedures. The samples (4 × 4 mm, thickness 2 mm; n = 72) were separated from
zirconia blanks (3Y-TZP-LA). One group was veenered with ceramics, and the other group of samples
was CAD/CAM milled. Each group had two subgroups: polished and glazed. The samples were
subjected to simulated strokes of professional brushing using abrasive paste and ultrasonic scaling.
The parameters of surface micromorphology and receptivity to biofilm were calculated before and
after simulating the given methods of the professional maintenance of oral hygiene. Scanning electron
microscopy (SEM) was used to evaluate zirconia surface properties. Microbial (bacterial/fungal)
species (Staphylococcus aureus, Streptococcus sanguinis and Candida albicans) were used and cultured
on respective sterilized zirconia surfaces. Colony-forming unit (CFU) counts were used to quantify
the amount of biofilm formation on zirconia samples surfaces. Results: The SEM analysis showed
the greatest change in surface microtopography after the use of ultrasonic scaling on glazed zirconia
samples. Less formation of colonies on the surfaces of CAD/CAM milled zirconia restorations was
observed. Conclusion: Routine methods of oral hygiene professional maintenance can damage the
surfaces of glazed zirconia restorations.",
publisher = "Basel: MDPI",
journal = "Applied Sciences",
title = "Biofilm Adhesion and Micromorphology Analysis after Professional Oral Hygiene Procedures on CAD/CAM Milled and Veneered Zirconia Restorations: In Vitro Study",
number = "14",
volume = "13",
doi = "10.3390/app13148389",
pages = "8389"
}

Perić, M., Čairović, A., Špadijer Gostović, A., Stanimirović, D., Kostić, M., Ivanov, M., Ćirić, A., Glamočlija, J.,& Pavlović, V. B.. (2023). Biofilm Adhesion and Micromorphology Analysis after Professional Oral Hygiene Procedures on CAD/CAM Milled and Veneered Zirconia Restorations: In Vitro Study. in Applied Sciences
Basel: MDPI., 13(14), 8389.
https://doi.org/10.3390/app13148389

Perić M, Čairović A, Špadijer Gostović A, Stanimirović D, Kostić M, Ivanov M, Ćirić A, Glamočlija J, Pavlović VB. Biofilm Adhesion and Micromorphology Analysis after Professional Oral Hygiene Procedures on CAD/CAM Milled and Veneered Zirconia Restorations: In Vitro Study. in Applied Sciences. 2023;13(14):8389.
doi:10.3390/app13148389 .

Perić, Mirjana, Čairović, Aleksandra, Špadijer Gostović, Aleksandra, Stanimirović, Dragan, Kostić, Marina, Ivanov, Marija, Ćirić, Ana, Glamočlija, Jasmina, Pavlović, Vladimir B., "Biofilm Adhesion and Micromorphology Analysis after Professional Oral Hygiene Procedures on CAD/CAM Milled and Veneered Zirconia Restorations: In Vitro Study" in Applied Sciences, 13, no. 14 (2023):8389,
https://doi.org/10.3390/app13148389 . .

TY  - JOUR
AU  - Perović, Tatjana
AU  - Petrović, Jovana
AU  - Gašić, Uroš
AU  - Kostić, Marina
AU  - Ćirić, Ana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5498
AB  - Plant extracts and other plant products have been used as an alternative to synthetic fungicides or an additional way to reduce their use. The choice of plant extracts and their application depends on their functional characteristics, availability, cost-effectiveness and their impact on phytopathogens, and also on the environment. Therefore, the present study aims to assess the potential of Celtis australis methanolic extracts as source of compounds with antifungal activity. Methanolic extracts prepared from leaves and unripe mesocarps of C. australis collected from different localities of Montenegro (Podgorica-PG, Donja Gorica-DG, and Bar-BR), were evaluated for their phenolic compounds composition as well as antifungal and cytotoxic properties. Obtained results revealed that extracts contain various bioactive constituents including phenolic acids, flavonoids and their derivatives. The predominant phenolic acid was ferulic acid, identified in leaves samples from DG (187.97 mg/100g dw), while isoorientin was the most abundant phenolic compound found in all examined samples. Regarding antifungal potential of the tested samples, all but one (prepared from mesocarp BR) possessed higher activity, than Previcur, commercial systemic fungicide intended to control seedling. In vitro studies on HaCaT cell line showed that the extracts had no toxic effect towards the tested cell line. These results lead to the conclusion that methanolic extracts of C. australis can become an alternative to the use of synthetic fungicides in agriculture. Those extracts represent natural biodegradable fungicides and enable a more efficient control of pathogenic fungi.
PB  - John Wiley and Sons Inc
T2  - Food Science and Nutrition
T1  - Natural extracts against agricultural pathogens: A case study of Celtis australis L.
DO  - 10.1002/fsn3.3325
ER  - 

@article{
author = "Perović, Tatjana and Petrović, Jovana and Gašić, Uroš and Kostić, Marina and Ćirić, Ana",
year = "2023",
abstract = "Plant extracts and other plant products have been used as an alternative to synthetic fungicides or an additional way to reduce their use. The choice of plant extracts and their application depends on their functional characteristics, availability, cost-effectiveness and their impact on phytopathogens, and also on the environment. Therefore, the present study aims to assess the potential of Celtis australis methanolic extracts as source of compounds with antifungal activity. Methanolic extracts prepared from leaves and unripe mesocarps of C. australis collected from different localities of Montenegro (Podgorica-PG, Donja Gorica-DG, and Bar-BR), were evaluated for their phenolic compounds composition as well as antifungal and cytotoxic properties. Obtained results revealed that extracts contain various bioactive constituents including phenolic acids, flavonoids and their derivatives. The predominant phenolic acid was ferulic acid, identified in leaves samples from DG (187.97 mg/100g dw), while isoorientin was the most abundant phenolic compound found in all examined samples. Regarding antifungal potential of the tested samples, all but one (prepared from mesocarp BR) possessed higher activity, than Previcur, commercial systemic fungicide intended to control seedling. In vitro studies on HaCaT cell line showed that the extracts had no toxic effect towards the tested cell line. These results lead to the conclusion that methanolic extracts of C. australis can become an alternative to the use of synthetic fungicides in agriculture. Those extracts represent natural biodegradable fungicides and enable a more efficient control of pathogenic fungi.",
publisher = "John Wiley and Sons Inc",
journal = "Food Science and Nutrition",
title = "Natural extracts against agricultural pathogens: A case study of Celtis australis L.",
doi = "10.1002/fsn3.3325"
}

Perović, T., Petrović, J., Gašić, U., Kostić, M.,& Ćirić, A.. (2023). Natural extracts against agricultural pathogens: A case study of Celtis australis L.. in Food Science and Nutrition
John Wiley and Sons Inc..
https://doi.org/10.1002/fsn3.3325

Perović T, Petrović J, Gašić U, Kostić M, Ćirić A. Natural extracts against agricultural pathogens: A case study of Celtis australis L.. in Food Science and Nutrition. 2023;.
doi:10.1002/fsn3.3325 .

Perović, Tatjana, Petrović, Jovana, Gašić, Uroš, Kostić, Marina, Ćirić, Ana, "Natural extracts against agricultural pathogens: A case study of Celtis australis L." in Food Science and Nutrition (2023),
https://doi.org/10.1002/fsn3.3325 . .

TY  - JOUR
AU  - Fernandes, Ângela
AU  - Chaski, Christina
AU  - Pereira, Carla
AU  - Kostić, Marina
AU  - Rouphael, Youssef
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Petropoulos, Spyridon A.
PY  - 2022
UR  - https://www.mdpi.com/2311-7524/8/7/645
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5090
AB  - The aim of the present study was to evaluate the effects of three biostimulant products (Nomoren (N), Twin Antistress (TW), x-Stress (XS) and control treatment (C: no biostimulants added)) on the nutritional value, chemical composition and bioactive properties of greenhouse tomato fruit grown under full (W+: 100% of field capacity) and deficit irrigation (W–: 70% of field capacity) conditions. Fat content was the highest for the fully irrigated plants that received no biostimulants (CW+), while proteins and carbohydrates and energetic value were the highest in the XSW+ treatment. The content of the main detected sugars (fructose, glucose and trehalose) varied depending on the irrigation and biostimulant treatment. The highest amounts of individual and total organic acids and tocopherols were recorded in fully irrigated plants treated with Twin Antistress (TW), whereas the lowest overall values were observed under deficit irrigation for plants that received the XS treatment. The most abundant fatty acids were palmitic (27.5–36.0%) and linoleic acid (27.4–35.4%), followed by oleic (9.2–21.2%), linolenic (5.4–13.1%) and stearic acid (5.3–6.8%). Moreover, the highest values of β-carotene and lycopene were recorded for the CW- and NW+ treatments, respectively. The TWW+ showed the highest antioxidant activity for both assays tested (TBARS and OxHLIA). Most of the tested extracts showed lower antibacterial activity against the tested bacteria compared to the positive controls. On the other hand, CW+, XSW+ and XSW- treatments showed higher antifungal activity (MIC values) than positive controls. In conclusion, each biostimulant product had a different effect on the determined characteristics depending on the level of irrigation. Therefore, more research is needed to better identify the mechanisms of action and the physiological processes, after which the tested biostimulants may be used to standardize the application of such products in tomato cultivation.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit
IS  - 7
VL  - 8
DO  - 10.3390/horticulturae8070645
SP  - 645
ER  - 

@article{
author = "Fernandes, Ângela and Chaski, Christina and Pereira, Carla and Kostić, Marina and Rouphael, Youssef and Soković, Marina and Barros, Lillian and Petropoulos, Spyridon A.",
year = "2022",
abstract = "The aim of the present study was to evaluate the effects of three biostimulant products (Nomoren (N), Twin Antistress (TW), x-Stress (XS) and control treatment (C: no biostimulants added)) on the nutritional value, chemical composition and bioactive properties of greenhouse tomato fruit grown under full (W+: 100% of field capacity) and deficit irrigation (W–: 70% of field capacity) conditions. Fat content was the highest for the fully irrigated plants that received no biostimulants (CW+), while proteins and carbohydrates and energetic value were the highest in the XSW+ treatment. The content of the main detected sugars (fructose, glucose and trehalose) varied depending on the irrigation and biostimulant treatment. The highest amounts of individual and total organic acids and tocopherols were recorded in fully irrigated plants treated with Twin Antistress (TW), whereas the lowest overall values were observed under deficit irrigation for plants that received the XS treatment. The most abundant fatty acids were palmitic (27.5–36.0%) and linoleic acid (27.4–35.4%), followed by oleic (9.2–21.2%), linolenic (5.4–13.1%) and stearic acid (5.3–6.8%). Moreover, the highest values of β-carotene and lycopene were recorded for the CW- and NW+ treatments, respectively. The TWW+ showed the highest antioxidant activity for both assays tested (TBARS and OxHLIA). Most of the tested extracts showed lower antibacterial activity against the tested bacteria compared to the positive controls. On the other hand, CW+, XSW+ and XSW- treatments showed higher antifungal activity (MIC values) than positive controls. In conclusion, each biostimulant product had a different effect on the determined characteristics depending on the level of irrigation. Therefore, more research is needed to better identify the mechanisms of action and the physiological processes, after which the tested biostimulants may be used to standardize the application of such products in tomato cultivation.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit",
number = "7",
volume = "8",
doi = "10.3390/horticulturae8070645",
pages = "645"
}

Fernandes, Â., Chaski, C., Pereira, C., Kostić, M., Rouphael, Y., Soković, M., Barros, L.,& Petropoulos, S. A.. (2022). Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit. in Horticulturae
Basel: MDPI., 8(7), 645.
https://doi.org/10.3390/horticulturae8070645

Fernandes Â, Chaski C, Pereira C, Kostić M, Rouphael Y, Soković M, Barros L, Petropoulos SA. Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit. in Horticulturae. 2022;8(7):645.
doi:10.3390/horticulturae8070645 .

Fernandes, Ângela, Chaski, Christina, Pereira, Carla, Kostić, Marina, Rouphael, Youssef, Soković, Marina, Barros, Lillian, Petropoulos, Spyridon A., "Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit" in Horticulturae, 8, no. 7 (2022):645,
https://doi.org/10.3390/horticulturae8070645 . .

TY  - CONF
AU  - Stojković, Dejan
AU  - Ivanov, Marija
AU  - Ćirić, Ana
AU  - Petrović, Jovana
AU  - Kostić, Marina
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5158
AB  - Chicory (Cichorium intybus L.) is an important industrial crop cultivated mainly to extract the
dietary fiber inulin. This study aimed to investigate antimicrobial, antibiofilm and wound healing
potentials of the extract obtained by supercritical fluid extraction (SFE). Antibacterial activity
of extract ranged from 0.25 to 4.00 mg/mL regarding minimal inhibitory concentra-tions (MICs),
while minimal bactericidal concentrations (MBCs) were noted within the range of 0.5 to 8.00 mg/
mL. The extract has shown promising antifungal potential with MICs rang-ing 0.125- 0.75 mg/
mL and MFCs 0.25-1.0 mg/mL against Candida species. Incubation with the extract has reduced
the ability of both C. albicans and P. aeruginosa to attach to the sur-face and begin the process
of biofilm formation. Concentration equal to previously deter-mined MIC has almost completely
(>90% inhibition) reduced the biofilm biomass formed by P. aeruginosa. Even twice as lower concentrations
(0.5 MIC) has limited the biofilm forming ability and induce more than 80% inhibition
in P. aeruginosa as bacterial biofilm model or-ganism. Impact on fungal biofilm was less profound
and MIC concentration was able to re-duce the biofilm formation for less than 60%. HaCaT (human
immortalized keratinocytes cell line) cells were treated with IC20 (24 h) concentration of chicory
SFE extract. It is estimated that SFE extract has slight effect on wound closure in keratinocyte
monolayer, when com-pared to untreated control. Since, Chicory SFE fraction possessed excellent
antibacterial po-tential against E. coli and K. pneumoniae and antifungal activity against Candida
species these results are considered very important in the future inventions
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia
T1  - Supercritical fluid extraction of Chicory reveals its antimicrobial, antibiofilm and wound healing potentials
SP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5158
ER  - 

@conference{
author = "Stojković, Dejan and Ivanov, Marija and Ćirić, Ana and Petrović, Jovana and Kostić, Marina and Glamočlija, Jasmina and Soković, Marina",
year = "2022",
abstract = "Chicory (Cichorium intybus L.) is an important industrial crop cultivated mainly to extract the
dietary fiber inulin. This study aimed to investigate antimicrobial, antibiofilm and wound healing
potentials of the extract obtained by supercritical fluid extraction (SFE). Antibacterial activity
of extract ranged from 0.25 to 4.00 mg/mL regarding minimal inhibitory concentra-tions (MICs),
while minimal bactericidal concentrations (MBCs) were noted within the range of 0.5 to 8.00 mg/
mL. The extract has shown promising antifungal potential with MICs rang-ing 0.125- 0.75 mg/
mL and MFCs 0.25-1.0 mg/mL against Candida species. Incubation with the extract has reduced
the ability of both C. albicans and P. aeruginosa to attach to the sur-face and begin the process
of biofilm formation. Concentration equal to previously deter-mined MIC has almost completely
(>90% inhibition) reduced the biofilm biomass formed by P. aeruginosa. Even twice as lower concentrations
(0.5 MIC) has limited the biofilm forming ability and induce more than 80% inhibition
in P. aeruginosa as bacterial biofilm model or-ganism. Impact on fungal biofilm was less profound
and MIC concentration was able to re-duce the biofilm formation for less than 60%. HaCaT (human
immortalized keratinocytes cell line) cells were treated with IC20 (24 h) concentration of chicory
SFE extract. It is estimated that SFE extract has slight effect on wound closure in keratinocyte
monolayer, when com-pared to untreated control. Since, Chicory SFE fraction possessed excellent
antibacterial po-tential against E. coli and K. pneumoniae and antifungal activity against Candida
species these results are considered very important in the future inventions",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia",
title = "Supercritical fluid extraction of Chicory reveals its antimicrobial, antibiofilm and wound healing potentials",
pages = "96",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5158"
}

Stojković, D., Ivanov, M., Ćirić, A., Petrović, J., Kostić, M., Glamočlija, J.,& Soković, M.. (2022). Supercritical fluid extraction of Chicory reveals its antimicrobial, antibiofilm and wound healing potentials. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 96.
https://hdl.handle.net/21.15107/rcub_ibiss_5158

Stojković D, Ivanov M, Ćirić A, Petrović J, Kostić M, Glamočlija J, Soković M. Supercritical fluid extraction of Chicory reveals its antimicrobial, antibiofilm and wound healing potentials. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. 2022;:96.
https://hdl.handle.net/21.15107/rcub_ibiss_5158 .

Stojković, Dejan, Ivanov, Marija, Ćirić, Ana, Petrović, Jovana, Kostić, Marina, Glamočlija, Jasmina, Soković, Marina, "Supercritical fluid extraction of Chicory reveals its antimicrobial, antibiofilm and wound healing potentials" in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia (2022):96,
https://hdl.handle.net/21.15107/rcub_ibiss_5158 .

TY  - JOUR
AU  - Ivanov, Marija
AU  - Kostić, Marina
AU  - Stojković, Dejan
AU  - Soković, Marina
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S025462992100497X
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4748
AB  - Emerging antimicrobial resistance and side effects associated with antibiotics overuse are driving the need for the novel antimicrobial therapeutics with the natural compounds considered as an appealing alternative. Rosmarinic acid is abundantly present in medicinal plants. This study aimed to elucidate its possible role as an inhibitor of planktonic and biofilm microorganism growth, along with its antifungal mechanisms. In this study, rosmarinic acid showed promising anticandidal (MIC 0.1–0.2 mg/mL) and antibacterial (MIC 0.002->0.8 mg/mL) activity. To some extent it was able to prevent cell attachment and eradicate established biofilms. Rosmarinic acid antifungal mechanisms included reduction of mitochondrial activity, alteration in membrane integrity, and slight inhibition of protease production but not binding to ergosterol. Its antibiofilm activity was moderately linked to the reduction in exopolysaccharide production. Considering its wide antimicrobial and antibiofilm spectrum rosmarinic acid could be further explored as an antimicrobial agent along with range of medicinal plants with rosmarinic acid as the dominant compound. Nevertheless, rosmarinic acid could serve as the basis for the development of antimicrobials employing novel mechanisms of activity.
T2  - South African Journal of Botany
T1  - Rosmarinic acid–Modes of antimicrobial and antibiofilm activities of common plant polyphenol
VL  - 146
DO  - 10.1016/j.sajb.2021.11.050
SP  - 521
EP  - 527
ER  - 

@article{
author = "Ivanov, Marija and Kostić, Marina and Stojković, Dejan and Soković, Marina",
year = "2022",
abstract = "Emerging antimicrobial resistance and side effects associated with antibiotics overuse are driving the need for the novel antimicrobial therapeutics with the natural compounds considered as an appealing alternative. Rosmarinic acid is abundantly present in medicinal plants. This study aimed to elucidate its possible role as an inhibitor of planktonic and biofilm microorganism growth, along with its antifungal mechanisms. In this study, rosmarinic acid showed promising anticandidal (MIC 0.1–0.2 mg/mL) and antibacterial (MIC 0.002->0.8 mg/mL) activity. To some extent it was able to prevent cell attachment and eradicate established biofilms. Rosmarinic acid antifungal mechanisms included reduction of mitochondrial activity, alteration in membrane integrity, and slight inhibition of protease production but not binding to ergosterol. Its antibiofilm activity was moderately linked to the reduction in exopolysaccharide production. Considering its wide antimicrobial and antibiofilm spectrum rosmarinic acid could be further explored as an antimicrobial agent along with range of medicinal plants with rosmarinic acid as the dominant compound. Nevertheless, rosmarinic acid could serve as the basis for the development of antimicrobials employing novel mechanisms of activity.",
journal = "South African Journal of Botany",
title = "Rosmarinic acid–Modes of antimicrobial and antibiofilm activities of common plant polyphenol",
volume = "146",
doi = "10.1016/j.sajb.2021.11.050",
pages = "521-527"
}

Ivanov, M., Kostić, M., Stojković, D.,& Soković, M.. (2022). Rosmarinic acid–Modes of antimicrobial and antibiofilm activities of common plant polyphenol. in South African Journal of Botany, 146, 521-527.
https://doi.org/10.1016/j.sajb.2021.11.050

Ivanov M, Kostić M, Stojković D, Soković M. Rosmarinic acid–Modes of antimicrobial and antibiofilm activities of common plant polyphenol. in South African Journal of Botany. 2022;146:521-527.
doi:10.1016/j.sajb.2021.11.050 .

Ivanov, Marija, Kostić, Marina, Stojković, Dejan, Soković, Marina, "Rosmarinic acid–Modes of antimicrobial and antibiofilm activities of common plant polyphenol" in South African Journal of Botany, 146 (2022):521-527,
https://doi.org/10.1016/j.sajb.2021.11.050 . .

TY  - CONF
AU  - Ćirić, Ana
AU  - Ivanov, Marija
AU  - Stojković, Dejan
AU  - Glamočlija, Jasmina
AU  - Kostić, Marina
AU  - Petrović, Jovana
AU  - Soković, Marina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5581
AB  - Cichorium intybus L. (Asteraceae), is an important medicinal plant, rich in sesquiterpene
lactones (STLs), inulin, biologically relevant elements, and vitamins which exert potent
pro-health effects on the human organism. The aim of this study was to screen two
extracts of chicory containing STLs, the optimized extract (Ci_SFEopt) and the
supercritical fluid extract (Ci_SFEpur), for their antimicrobial activities against a range
of pathogenic microorganisms. Both extracts showed antibacterial activity, with
Ci_SFEpur being more potent than Ci_SFEopt. The most sensitive species was
Pseudomonas aeruginosa (minimal inhibitory concentration, MIC 0.06 mg/mL).
Ci_SFEpur and Ci_SFEopt extracts demonstrated good antifungal activity against
Candida species (MIC 0.03-1.00 mg/mL). Thin layer chromatography (TLC)
bioautography was used to screen the extracts and provide basic information for the
isolation of bioactive compounds in the future. Ci_SFEopt was active against all tested
microorganisms (Rf values were 0.18-0.88). The Ci_SFEpur extract efficiently inhibited
(Rf 0.76) Staphylococcus aureus, P. aeruginosa and Candida krusei. The identification
of powerful antimicrobial activity of Ci_SFEpur against P. aeruginosa and Candida
species is an important step towards the development of novel antimicrobial therapeutics
and efficient utilization of this widely distributed plant.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Evaluation of antimicrobial properties of chicory extracts
SP  - 257
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5581
ER  - 

@conference{
author = "Ćirić, Ana and Ivanov, Marija and Stojković, Dejan and Glamočlija, Jasmina and Kostić, Marina and Petrović, Jovana and Soković, Marina",
year = "2022",
abstract = "Cichorium intybus L. (Asteraceae), is an important medicinal plant, rich in sesquiterpene
lactones (STLs), inulin, biologically relevant elements, and vitamins which exert potent
pro-health effects on the human organism. The aim of this study was to screen two
extracts of chicory containing STLs, the optimized extract (Ci_SFEopt) and the
supercritical fluid extract (Ci_SFEpur), for their antimicrobial activities against a range
of pathogenic microorganisms. Both extracts showed antibacterial activity, with
Ci_SFEpur being more potent than Ci_SFEopt. The most sensitive species was
Pseudomonas aeruginosa (minimal inhibitory concentration, MIC 0.06 mg/mL).
Ci_SFEpur and Ci_SFEopt extracts demonstrated good antifungal activity against
Candida species (MIC 0.03-1.00 mg/mL). Thin layer chromatography (TLC)
bioautography was used to screen the extracts and provide basic information for the
isolation of bioactive compounds in the future. Ci_SFEopt was active against all tested
microorganisms (Rf values were 0.18-0.88). The Ci_SFEpur extract efficiently inhibited
(Rf 0.76) Staphylococcus aureus, P. aeruginosa and Candida krusei. The identification
of powerful antimicrobial activity of Ci_SFEpur against P. aeruginosa and Candida
species is an important step towards the development of novel antimicrobial therapeutics
and efficient utilization of this widely distributed plant.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Evaluation of antimicrobial properties of chicory extracts",
pages = "257",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5581"
}

Ćirić, A., Ivanov, M., Stojković, D., Glamočlija, J., Kostić, M., Petrović, J.,& Soković, M.. (2022). Evaluation of antimicrobial properties of chicory extracts. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 257.
https://hdl.handle.net/21.15107/rcub_ibiss_5581

Ćirić A, Ivanov M, Stojković D, Glamočlija J, Kostić M, Petrović J, Soković M. Evaluation of antimicrobial properties of chicory extracts. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:257.
https://hdl.handle.net/21.15107/rcub_ibiss_5581 .

Ćirić, Ana, Ivanov, Marija, Stojković, Dejan, Glamočlija, Jasmina, Kostić, Marina, Petrović, Jovana, Soković, Marina, "Evaluation of antimicrobial properties of chicory extracts" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):257,
https://hdl.handle.net/21.15107/rcub_ibiss_5581 .

TY  - CONF
AU  - Kostić, Marina
AU  - Petrović, Jovana
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5282
AB  - Veliki broj makrogljiva predstavlja nedovoljno iskorišćen izvor jedinjenja koja mogu imati potencijalnu primenu u medicini, farmaciji i različitim granama privrede. Pored toga što imaju povoljan odnos makronutrijenata, važan su izvor visoko cenjenih polisaharida, lektina, terpenoida, peptida, itd. Ova jedinjenja su odgovorna za mnogobrojne biološke aktivnosti uključujući antineurodegenerativnu, imunomodulatornu, antimikrobnu, antihiperglikemijsku aktivnost. Danas su u upotrebi različiti dijetetski proizvodi na bazi makrogljiva koji se koriste za prevenciju i ublažavanje simptoma različitih oboljenja. Iako ovi proizvodi ne mogu u potpunosti zameniti komercijalne lekove, u kombinaciji sa njihovom primenom mogu da dovedu do poboljšanja opšteg stanja. Zbog navedenog, autohtone samonikle, ali i komercijalno gajene vrste makrogljiva predstavljaju važan i još uvek nedovoljno istražen izvor bioloških aktivnih supstanci. Štaviše, one imaju ogroman potencijal kao profilaktički agensi kod bolesti čiji je osnovni uzrok povezan sa oksidativnim stresom, bakterijskim i gljivičnim infekcijama kao i pojavom/razvojem različitih tipova tumora. Imajući u vidu da su hranljiva i lekovita svojstva jestivih i lekovitih gljiva hvaljena kako u etnomedicini tako i u naučnim/kliničkim istraživanjima, ova svojstva će biti predstavljena kako bi se procenili novi trendovi i moguća primena jestivih i lekovitih gljiva sa teritorije Srbije u medicini, farmaceutskoj i prehrambenoj industriji.
AB  - Велики број макрогљива представља недовољно искоришћен извор једињења која могу имати потенцијалну примену у медицини, фармацији и различитим гранама привреде. Поред тога што имају повољан однос макронутријената, важан су извор високо цењених полисахарида, лектина, терпеноида, пептида, итд. Ова једињења су одговорна за многобројне биолошке активности укључујући антинеуродегенеративну, имуномодулаторну, антимикробну, антихипергликемијску активност. Данас су у употреби различити дијeтетски производи на бази макрогљива који се користе за превенцију и ублажавање симптома различитих обољења. Иако ови производи не могу у потпуности заменити комерцијалне лекове, у комбинацији са њиховом применом могу да доведу до побољшања општег стања. Због наведеног, аутохтоне самоникле, али и комерцијално гајене врсте макрогљива представљају важан и још увек недовољно истражен извор биолошких активних супстанци. Штавише, оне имају огроман потенцијал као профилактички агенси код болести чији је основни узрок повезан са оксидативним стресом, бактеријским и гљивичним инфекцијама као и појавом/развојем различитих типова тумора. Имајући у виду да су хранљива и лековита својства јестивих и лековитих гљива хваљена како у етномедицини тако и у научним/клиничким истраживањима, ова својства ће бити представљена како би се проценили нови трендови и могућа примена јестивих и лековитих гљива са територије Србије у медицини, фармацеутској и прехрамбеној индустрији.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Biološki potencijal gljiva sa teritorije Srbije
T1  - Биолошки потенцијал гљива са територије Србије
SP  - 227
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5282
ER  - 

@conference{
author = "Kostić, Marina and Petrović, Jovana and Glamočlija, Jasmina and Soković, Marina",
year = "2022",
abstract = "Veliki broj makrogljiva predstavlja nedovoljno iskorišćen izvor jedinjenja koja mogu imati potencijalnu primenu u medicini, farmaciji i različitim granama privrede. Pored toga što imaju povoljan odnos makronutrijenata, važan su izvor visoko cenjenih polisaharida, lektina, terpenoida, peptida, itd. Ova jedinjenja su odgovorna za mnogobrojne biološke aktivnosti uključujući antineurodegenerativnu, imunomodulatornu, antimikrobnu, antihiperglikemijsku aktivnost. Danas su u upotrebi različiti dijetetski proizvodi na bazi makrogljiva koji se koriste za prevenciju i ublažavanje simptoma različitih oboljenja. Iako ovi proizvodi ne mogu u potpunosti zameniti komercijalne lekove, u kombinaciji sa njihovom primenom mogu da dovedu do poboljšanja opšteg stanja. Zbog navedenog, autohtone samonikle, ali i komercijalno gajene vrste makrogljiva predstavljaju važan i još uvek nedovoljno istražen izvor bioloških aktivnih supstanci. Štaviše, one imaju ogroman potencijal kao profilaktički agensi kod bolesti čiji je osnovni uzrok povezan sa oksidativnim stresom, bakterijskim i gljivičnim infekcijama kao i pojavom/razvojem različitih tipova tumora. Imajući u vidu da su hranljiva i lekovita svojstva jestivih i lekovitih gljiva hvaljena kako u etnomedicini tako i u naučnim/kliničkim istraživanjima, ova svojstva će biti predstavljena kako bi se procenili novi trendovi i moguća primena jestivih i lekovitih gljiva sa teritorije Srbije u medicini, farmaceutskoj i prehrambenoj industriji., Велики број макрогљива представља недовољно искоришћен извор једињења која могу имати потенцијалну примену у медицини, фармацији и различитим гранама привреде. Поред тога што имају повољан однос макронутријената, важан су извор високо цењених полисахарида, лектина, терпеноида, пептида, итд. Ова једињења су одговорна за многобројне биолошке активности укључујући антинеуродегенеративну, имуномодулаторну, антимикробну, антихипергликемијску активност. Данас су у употреби различити дијeтетски производи на бази макрогљива који се користе за превенцију и ублажавање симптома различитих обољења. Иако ови производи не могу у потпуности заменити комерцијалне лекове, у комбинацији са њиховом применом могу да доведу до побољшања општег стања. Због наведеног, аутохтоне самоникле, али и комерцијално гајене врсте макрогљива представљају важан и још увек недовољно истражен извор биолошких активних супстанци. Штавише, оне имају огроман потенцијал као профилактички агенси код болести чији је основни узрок повезан са оксидативним стресом, бактеријским и гљивичним инфекцијама као и појавом/развојем различитих типова тумора. Имајући у виду да су хранљива и лековита својства јестивих и лековитих гљива хваљена како у етномедицини тако и у научним/клиничким истраживањима, ова својства ће бити представљена како би се проценили нови трендови и могућа примена јестивих и лековитих гљива са територије Србије у медицини, фармацеутској и прехрамбеној индустрији.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Biološki potencijal gljiva sa teritorije Srbije, Биолошки потенцијал гљива са територије Србије",
pages = "227",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5282"
}

Kostić, M., Petrović, J., Glamočlija, J.,& Soković, M.. (2022). Biološki potencijal gljiva sa teritorije Srbije. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 227.
https://hdl.handle.net/21.15107/rcub_ibiss_5282

Kostić M, Petrović J, Glamočlija J, Soković M. Biološki potencijal gljiva sa teritorije Srbije. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:227.
https://hdl.handle.net/21.15107/rcub_ibiss_5282 .

Kostić, Marina, Petrović, Jovana, Glamočlija, Jasmina, Soković, Marina, "Biološki potencijal gljiva sa teritorije Srbije" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):227,
https://hdl.handle.net/21.15107/rcub_ibiss_5282 .

TY  - CONF
AU  - Kostić, Marina
AU  - Ivanov, Marija
AU  - Ćirić, Ana
AU  - Caleja, Cristina
AU  - Pereira, Eliana
AU  - Barros, Lillian
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5156
AB  - Mushrooms are highly appreciated for their nutritional value and therapeutic effects. They have found wide application in pharmacy, medicine and as nutraceuticals, mostly due to the synergistic activity of their bioactive compounds. This study aimed to investigate the chemical composition and antioxidant, antibacterial, antibiofilm, and cytotoxic potential of L. piperatus and L. vellereus wild growing mushrooms from Serbia. Mannitol and trehalose were the most abundant free sugars and malic and citric acid were the major organic acids detected. The four tocopherol isoforms were found in L. piperatus, and the β-tocopherol was the only tocopherol isoform found in the L. vellereus fruiting body. Regarding phenolic compounds, cinnamic and protocatechinic acids were identified in the prepared methanolic and ethanolic extracts, which displayed antioxidant activity through the inhibition of thiobarbituric acid reactive substances (TBARS) formation (IC50 0.30-1.39 mg/mL). Antibacterial activity of Lactarius ethanolic and methanolic extracts was investigated against bacteria isolated from tonsils from the patient with chronic tonsillitis. All tested extracts demonstrated promising results regarding antibacterial and antibiofim activity. In both extracts, the most promising activity was observed for Streptococcus spp. (0.40–6.25 mg/mL as the minimal inhibitory concentration). Ethanolic extracts of tested mushrooms showed a stronger potential to inhibit the formation of Staphylococcus aureus biofilm then methanolic. L. vellereus ethanolic extract showed highest inhibition activity (86%) against S. aureus biofilm formation. Citotoxic potential of L. piperatus and L. vellereus extracts were examined against four tumor cell lines (HeLa, MCF-7, HepG2, NCI-H460) and porcine liver primary culture - PLP2. The most promising cytotoxic effect was caused by the L. vellereus ethanolic extract on cervical carcinoma cell line (HeLa). However, extensive screening of biological activities is needed in future studies that will allow the exploitation of these mushrooms at an industrial level, taking advantage of its benefits for human health.
PB  - Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia
C3  - Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia
T1  - Chemical composition and biological activity of two Lactarius species from Serbia.
SP  - 102
EP  - 103
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5156
ER  - 

@conference{
author = "Kostić, Marina and Ivanov, Marija and Ćirić, Ana and Caleja, Cristina and Pereira, Eliana and Barros, Lillian and Stojković, Dejan and Soković, Marina and Glamočlija, Jasmina",
year = "2022",
abstract = "Mushrooms are highly appreciated for their nutritional value and therapeutic effects. They have found wide application in pharmacy, medicine and as nutraceuticals, mostly due to the synergistic activity of their bioactive compounds. This study aimed to investigate the chemical composition and antioxidant, antibacterial, antibiofilm, and cytotoxic potential of L. piperatus and L. vellereus wild growing mushrooms from Serbia. Mannitol and trehalose were the most abundant free sugars and malic and citric acid were the major organic acids detected. The four tocopherol isoforms were found in L. piperatus, and the β-tocopherol was the only tocopherol isoform found in the L. vellereus fruiting body. Regarding phenolic compounds, cinnamic and protocatechinic acids were identified in the prepared methanolic and ethanolic extracts, which displayed antioxidant activity through the inhibition of thiobarbituric acid reactive substances (TBARS) formation (IC50 0.30-1.39 mg/mL). Antibacterial activity of Lactarius ethanolic and methanolic extracts was investigated against bacteria isolated from tonsils from the patient with chronic tonsillitis. All tested extracts demonstrated promising results regarding antibacterial and antibiofim activity. In both extracts, the most promising activity was observed for Streptococcus spp. (0.40–6.25 mg/mL as the minimal inhibitory concentration). Ethanolic extracts of tested mushrooms showed a stronger potential to inhibit the formation of Staphylococcus aureus biofilm then methanolic. L. vellereus ethanolic extract showed highest inhibition activity (86%) against S. aureus biofilm formation. Citotoxic potential of L. piperatus and L. vellereus extracts were examined against four tumor cell lines (HeLa, MCF-7, HepG2, NCI-H460) and porcine liver primary culture - PLP2. The most promising cytotoxic effect was caused by the L. vellereus ethanolic extract on cervical carcinoma cell line (HeLa). However, extensive screening of biological activities is needed in future studies that will allow the exploitation of these mushrooms at an industrial level, taking advantage of its benefits for human health.",
publisher = "Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia",
journal = "Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia",
title = "Chemical composition and biological activity of two Lactarius species from Serbia.",
pages = "102-103",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5156"
}

Kostić, M., Ivanov, M., Ćirić, A., Caleja, C., Pereira, E., Barros, L., Stojković, D., Soković, M.,& Glamočlija, J.. (2022). Chemical composition and biological activity of two Lactarius species from Serbia.. in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia
Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia., 102-103.
https://hdl.handle.net/21.15107/rcub_ibiss_5156

Kostić M, Ivanov M, Ćirić A, Caleja C, Pereira E, Barros L, Stojković D, Soković M, Glamočlija J. Chemical composition and biological activity of two Lactarius species from Serbia.. in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia. 2022;:102-103.
https://hdl.handle.net/21.15107/rcub_ibiss_5156 .

Kostić, Marina, Ivanov, Marija, Ćirić, Ana, Caleja, Cristina, Pereira, Eliana, Barros, Lillian, Stojković, Dejan, Soković, Marina, Glamočlija, Jasmina, "Chemical composition and biological activity of two Lactarius species from Serbia." in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia (2022):102-103,
https://hdl.handle.net/21.15107/rcub_ibiss_5156 .

TY  - JOUR
AU  - Kostić, Marina
AU  - Ivanov, Marija
AU  - Sanković Babić, Snežana
AU  - Tepavčević, Zvezdana
AU  - Radanović, Oliver
AU  - Soković, Marina
AU  - Ćirić, Ana
PY  - 2022
UR  - https://www.mdpi.com/2079-6382/11/12/1747
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5353
AB  - Chronic tonsillitis (CT) is a global health issue which can impair patient’s quality of life and has an important socioeconomic impact due to the nonrational use of antibiotics, increased antimicrobial resistance and frequent need for surgical treatment. In order to isolate and identify the causing agents of CT, a total of 79 postoperative palatine and adenoid tissue samples were obtained from the ENT Clinic, KBC Zvezdara, Belgrade, Serbia. Culture identification was performed by MALDI-TOF MS and the Staphylococcus aureus isolates were tested for biofilm forming capability and antibiotic susceptibility. Additionally, a histological examination of palatine and adenoid tissue was performed in order to detect the presence of CT-causing bacteria. The slight majority of participants were females with median age of 28 years for adult patients (group I) and 6 years for children (group II). Analysis of the incidence of bacteria isolated from tissue samples in both groups showed the highest prevalence of S. aureus, Streptococcus oralis and Streptococcus parasanquinis. In addition to interfollicular hyperplasia, colonies of species S. aureus were detected in histological material. The presence of biofilm might be the reason for the recurrence of infection. Therefore, searching for a new treatment of CT is of great importance.
PB  - Basel: MDPI
T2  - Antibiotics
T1  - Analysis of Tonsil Tissues from Patients Diagnosed with Chronic Tonsillitis—Microbiological Profile, Biofilm-Forming Capacity and Histology
IS  - 12
VL  - 11
DO  - 10.3390/antibiotics11121747
SP  - 1747
ER  - 

@article{
author = "Kostić, Marina and Ivanov, Marija and Sanković Babić, Snežana and Tepavčević, Zvezdana and Radanović, Oliver and Soković, Marina and Ćirić, Ana",
year = "2022",
abstract = "Chronic tonsillitis (CT) is a global health issue which can impair patient’s quality of life and has an important socioeconomic impact due to the nonrational use of antibiotics, increased antimicrobial resistance and frequent need for surgical treatment. In order to isolate and identify the causing agents of CT, a total of 79 postoperative palatine and adenoid tissue samples were obtained from the ENT Clinic, KBC Zvezdara, Belgrade, Serbia. Culture identification was performed by MALDI-TOF MS and the Staphylococcus aureus isolates were tested for biofilm forming capability and antibiotic susceptibility. Additionally, a histological examination of palatine and adenoid tissue was performed in order to detect the presence of CT-causing bacteria. The slight majority of participants were females with median age of 28 years for adult patients (group I) and 6 years for children (group II). Analysis of the incidence of bacteria isolated from tissue samples in both groups showed the highest prevalence of S. aureus, Streptococcus oralis and Streptococcus parasanquinis. In addition to interfollicular hyperplasia, colonies of species S. aureus were detected in histological material. The presence of biofilm might be the reason for the recurrence of infection. Therefore, searching for a new treatment of CT is of great importance.",
publisher = "Basel: MDPI",
journal = "Antibiotics",
title = "Analysis of Tonsil Tissues from Patients Diagnosed with Chronic Tonsillitis—Microbiological Profile, Biofilm-Forming Capacity and Histology",
number = "12",
volume = "11",
doi = "10.3390/antibiotics11121747",
pages = "1747"
}

Kostić, M., Ivanov, M., Sanković Babić, S., Tepavčević, Z., Radanović, O., Soković, M.,& Ćirić, A.. (2022). Analysis of Tonsil Tissues from Patients Diagnosed with Chronic Tonsillitis—Microbiological Profile, Biofilm-Forming Capacity and Histology. in Antibiotics
Basel: MDPI., 11(12), 1747.
https://doi.org/10.3390/antibiotics11121747

Kostić M, Ivanov M, Sanković Babić S, Tepavčević Z, Radanović O, Soković M, Ćirić A. Analysis of Tonsil Tissues from Patients Diagnosed with Chronic Tonsillitis—Microbiological Profile, Biofilm-Forming Capacity and Histology. in Antibiotics. 2022;11(12):1747.
doi:10.3390/antibiotics11121747 .

Kostić, Marina, Ivanov, Marija, Sanković Babić, Snežana, Tepavčević, Zvezdana, Radanović, Oliver, Soković, Marina, Ćirić, Ana, "Analysis of Tonsil Tissues from Patients Diagnosed with Chronic Tonsillitis—Microbiological Profile, Biofilm-Forming Capacity and Histology" in Antibiotics, 11, no. 12 (2022):1747,
https://doi.org/10.3390/antibiotics11121747 . .

TY  - JOUR
AU  - Kostić, Marina
AU  - Ivanov, Marija
AU  - Marković, Tatjana
AU  - Sanković Babić, Snežana
AU  - Barros, Lillian
AU  - Calhelha, Ricardo
AU  - Soković, Marina
AU  - Ćirić, Ana
PY  - 2022
UR  - https://www.tandfonline.com/doi/full/10.1080/10412905.2022.2107103
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5101
AB  - The chemical composition and biological activity of essential oils (EOs) from leaves of Origanum minutiflorum O. Schwarz & P.H.Davis, and seeds of Coriandrum sativum L. were investigated. Chemical analyses of EOs were performed and the major components were carvacrol (81.5%) and linalool (69.6%), respectively. The antimicrobial activity was assessed against several bacteria originating from the tonsillar tissue. Activities of EOs against Staphylococcus aureus biofilm were investigated, as well as the effect of the mixture of these EOs and antibiotics against the pathogen. The antioxidant activity of both EOs was determined by TBARS assay, and examined wild oregano EO showed better activity. Also, cytotoxicity of EOs was evaluated in vitro and both EOs showed potential to inhibit further proliferation of tumor cells. This study reported for the first time the effects of EOs on chronic tonsillitis causative pathogens, supporting their role as a natural bioactive therapeutic agent with possible antimicrobial applications.
PB  - Taylor and Francis Ltd.
T2  - Journal of Essential Oil Research
T1  - An in vitro study of the Origanum minutiflorum O. Schwarz & P. H. Davis and Coriandrum sativum L. essential oils as chronic tonsillitis therapeutics: antibacterial, antibiofilm, antioxidant, and cytotoxic activities
IS  - 6
VL  - 34
DO  - 10.1080/10412905.2022.2107103
SP  - 533
EP  - 543
ER  - 

@article{
author = "Kostić, Marina and Ivanov, Marija and Marković, Tatjana and Sanković Babić, Snežana and Barros, Lillian and Calhelha, Ricardo and Soković, Marina and Ćirić, Ana",
year = "2022",
abstract = "The chemical composition and biological activity of essential oils (EOs) from leaves of Origanum minutiflorum O. Schwarz & P.H.Davis, and seeds of Coriandrum sativum L. were investigated. Chemical analyses of EOs were performed and the major components were carvacrol (81.5%) and linalool (69.6%), respectively. The antimicrobial activity was assessed against several bacteria originating from the tonsillar tissue. Activities of EOs against Staphylococcus aureus biofilm were investigated, as well as the effect of the mixture of these EOs and antibiotics against the pathogen. The antioxidant activity of both EOs was determined by TBARS assay, and examined wild oregano EO showed better activity. Also, cytotoxicity of EOs was evaluated in vitro and both EOs showed potential to inhibit further proliferation of tumor cells. This study reported for the first time the effects of EOs on chronic tonsillitis causative pathogens, supporting their role as a natural bioactive therapeutic agent with possible antimicrobial applications.",
publisher = "Taylor and Francis Ltd.",
journal = "Journal of Essential Oil Research",
title = "An in vitro study of the Origanum minutiflorum O. Schwarz & P. H. Davis and Coriandrum sativum L. essential oils as chronic tonsillitis therapeutics: antibacterial, antibiofilm, antioxidant, and cytotoxic activities",
number = "6",
volume = "34",
doi = "10.1080/10412905.2022.2107103",
pages = "533-543"
}

Kostić, M., Ivanov, M., Marković, T., Sanković Babić, S., Barros, L., Calhelha, R., Soković, M.,& Ćirić, A.. (2022). An in vitro study of the Origanum minutiflorum O. Schwarz & P. H. Davis and Coriandrum sativum L. essential oils as chronic tonsillitis therapeutics: antibacterial, antibiofilm, antioxidant, and cytotoxic activities. in Journal of Essential Oil Research
Taylor and Francis Ltd.., 34(6), 533-543.
https://doi.org/10.1080/10412905.2022.2107103

Kostić M, Ivanov M, Marković T, Sanković Babić S, Barros L, Calhelha R, Soković M, Ćirić A. An in vitro study of the Origanum minutiflorum O. Schwarz & P. H. Davis and Coriandrum sativum L. essential oils as chronic tonsillitis therapeutics: antibacterial, antibiofilm, antioxidant, and cytotoxic activities. in Journal of Essential Oil Research. 2022;34(6):533-543.
doi:10.1080/10412905.2022.2107103 .

Kostić, Marina, Ivanov, Marija, Marković, Tatjana, Sanković Babić, Snežana, Barros, Lillian, Calhelha, Ricardo, Soković, Marina, Ćirić, Ana, "An in vitro study of the Origanum minutiflorum O. Schwarz & P. H. Davis and Coriandrum sativum L. essential oils as chronic tonsillitis therapeutics: antibacterial, antibiofilm, antioxidant, and cytotoxic activities" in Journal of Essential Oil Research, 34, no. 6 (2022):533-543,
https://doi.org/10.1080/10412905.2022.2107103 . .

TY  - JOUR
AU  - Tratrat, Christophe
AU  - Petrou, Anthi
AU  - Geronikaki, Athina
AU  - Ivanov, Marija
AU  - Kostić, Marina
AU  - Soković, Marina
AU  - Vizirianakis, Ioannis S.
AU  - Theodoroula, Nikoleta F.
AU  - Haroun, Michelyne
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4936
AB  - Herein, we report computational and experimental evaluations of the antimicrobial activity of twenty one 2,3-diaryl-thiazolidin-4-ones. All synthesized compounds exhibited an antibacterial activity against six Gram-positive and Gram-negative bacteria to different extents. Thus, the MIC was in the range of 0.008–0.24 mg/mL, while the MBC was 0.0016–0.48 mg/mL. The most sensitive bacterium was S. Typhimurium, whereas S. aureus was the most resistant. The best antibacterial activity was observed for compound 5 (MIC at 0.008–0.06 mg/mL). The three most active compounds 5, 8, and 15, as well as compound 6, which were evaluated against three resistant strains, MRSA, P. aeruginosa, and E. coli, were more potent against all bacterial strains used than ampicillin. The antifungal activity of some compounds exceeded or were equipotent with those of the reference antifungal agents bifonazole and ketoconazole. The best activity was expressed by compound 5. All compounds exhibited moderate to good drug-likeness scores ranging from −0.39 to 0.39. The docking studies indicated a probable involvement of E. coli Mur B inhibition in the antibacterial action, while CYP51 inhibition is likely responsible for the antifungal activity of the tested compounds. Finally, the assessment of cellular cytotoxicity of the compounds in normal human MRC-5 cells revealed that the compounds were not toxic. View Full-Text
PB  - Basel: MDPI
T2  - Molecules
T1  - Thiazolidin-4-Ones as Potential Antimicrobial Agents: Experimental and In Silico Evaluation
IS  - 6
VL  - 27
DO  - 10.3390/molecules27061930
SP  - 1930
ER  - 

@article{
author = "Tratrat, Christophe and Petrou, Anthi and Geronikaki, Athina and Ivanov, Marija and Kostić, Marina and Soković, Marina and Vizirianakis, Ioannis S. and Theodoroula, Nikoleta F. and Haroun, Michelyne",
year = "2022",
abstract = "Herein, we report computational and experimental evaluations of the antimicrobial activity of twenty one 2,3-diaryl-thiazolidin-4-ones. All synthesized compounds exhibited an antibacterial activity against six Gram-positive and Gram-negative bacteria to different extents. Thus, the MIC was in the range of 0.008–0.24 mg/mL, while the MBC was 0.0016–0.48 mg/mL. The most sensitive bacterium was S. Typhimurium, whereas S. aureus was the most resistant. The best antibacterial activity was observed for compound 5 (MIC at 0.008–0.06 mg/mL). The three most active compounds 5, 8, and 15, as well as compound 6, which were evaluated against three resistant strains, MRSA, P. aeruginosa, and E. coli, were more potent against all bacterial strains used than ampicillin. The antifungal activity of some compounds exceeded or were equipotent with those of the reference antifungal agents bifonazole and ketoconazole. The best activity was expressed by compound 5. All compounds exhibited moderate to good drug-likeness scores ranging from −0.39 to 0.39. The docking studies indicated a probable involvement of E. coli Mur B inhibition in the antibacterial action, while CYP51 inhibition is likely responsible for the antifungal activity of the tested compounds. Finally, the assessment of cellular cytotoxicity of the compounds in normal human MRC-5 cells revealed that the compounds were not toxic. View Full-Text",
publisher = "Basel: MDPI",
journal = "Molecules",
title = "Thiazolidin-4-Ones as Potential Antimicrobial Agents: Experimental and In Silico Evaluation",
number = "6",
volume = "27",
doi = "10.3390/molecules27061930",
pages = "1930"
}

Tratrat, C., Petrou, A., Geronikaki, A., Ivanov, M., Kostić, M., Soković, M., Vizirianakis, I. S., Theodoroula, N. F.,& Haroun, M.. (2022). Thiazolidin-4-Ones as Potential Antimicrobial Agents: Experimental and In Silico Evaluation. in Molecules
Basel: MDPI., 27(6), 1930.
https://doi.org/10.3390/molecules27061930

Tratrat C, Petrou A, Geronikaki A, Ivanov M, Kostić M, Soković M, Vizirianakis IS, Theodoroula NF, Haroun M. Thiazolidin-4-Ones as Potential Antimicrobial Agents: Experimental and In Silico Evaluation. in Molecules. 2022;27(6):1930.
doi:10.3390/molecules27061930 .

Tratrat, Christophe, Petrou, Anthi, Geronikaki, Athina, Ivanov, Marija, Kostić, Marina, Soković, Marina, Vizirianakis, Ioannis S., Theodoroula, Nikoleta F., Haroun, Michelyne, "Thiazolidin-4-Ones as Potential Antimicrobial Agents: Experimental and In Silico Evaluation" in Molecules, 27, no. 6 (2022):1930,
https://doi.org/10.3390/molecules27061930 . .

TY  - CONF
AU  - Kostić, Marina
AU  - Ivanov, Marija
AU  - Sanković Babić, Snežana
AU  - Tapavčević, Zvezdana
AU  - Radanović, Oliver
AU  - Ćirić, Ana
AU  - Soković, Marina
PY  - 2022
UR  - https://www.femsbelgrade2022.org/abstract-book
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5155
AB  - If chronic tonsillitis (CT) is treated surgically, the long-term risks of infections and respiratory diseases are
higher, making of high importance to find an alternative for tonsillectomy. In order to isolate and identify
the causing agents of CT, a total of 79 postoperative palatine and adenoid tissue samples were obtained
from the ENT Clinic, KBC Zvezdara. A total of 82 bacterial species were isolated and identified by MALDI-TOF.
They belong to the genera Streptococcus, Staphylococcus, Micrococcus, Rothia, Enterobacter and Stenotrophomonas.
Analysis of the incidence of bacteria isolated from tissue samples showed the highest prevalence of Streptococcus
oralis (27/33%), followed by S. aureus (17/21%) and Streptococcus parasanquinis (16/20%). Histological analysis
of tissue samples was conducted in order to detect the presence of CT-causing bacteria. In addition to interfollicular
hyperplasia, colonies of species S. aureus and Stenotrophomonas maltophilia were detected in histological material.
The presented results confirm that the colonizers of tonsils tissues can be both G (+) and G (-) bacteria. The
presence of S. aureus in the tonsillar tissue even after the antibiotic therapy may be related to its ability to
form biofilm. Our study demonstrated that among 17 S. aureus isolates, 13 displays more pronounced biofilm
forming ability than the reference strain S. aureus ATCC 11632, and only 4 isolates display lower biofilm forming ability.
The presence of biofilm might be the reason for the recurrence of infection. Therefore, a searching for new
antibiofilm therapeutics in CT therapy is of great importance.
PB  - Belgrade: Serbian Society of Microbiology
C3  - Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia
T1  - Microbiological and histological analysis of tonsil tissue from patients diagnosed with chronic tonsilitis
SP  - 853
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5155
ER  - 

@conference{
author = "Kostić, Marina and Ivanov, Marija and Sanković Babić, Snežana and Tapavčević, Zvezdana and Radanović, Oliver and Ćirić, Ana and Soković, Marina",
year = "2022",
abstract = "If chronic tonsillitis (CT) is treated surgically, the long-term risks of infections and respiratory diseases are
higher, making of high importance to find an alternative for tonsillectomy. In order to isolate and identify
the causing agents of CT, a total of 79 postoperative palatine and adenoid tissue samples were obtained
from the ENT Clinic, KBC Zvezdara. A total of 82 bacterial species were isolated and identified by MALDI-TOF.
They belong to the genera Streptococcus, Staphylococcus, Micrococcus, Rothia, Enterobacter and Stenotrophomonas.
Analysis of the incidence of bacteria isolated from tissue samples showed the highest prevalence of Streptococcus
oralis (27/33%), followed by S. aureus (17/21%) and Streptococcus parasanquinis (16/20%). Histological analysis
of tissue samples was conducted in order to detect the presence of CT-causing bacteria. In addition to interfollicular
hyperplasia, colonies of species S. aureus and Stenotrophomonas maltophilia were detected in histological material.
The presented results confirm that the colonizers of tonsils tissues can be both G (+) and G (-) bacteria. The
presence of S. aureus in the tonsillar tissue even after the antibiotic therapy may be related to its ability to
form biofilm. Our study demonstrated that among 17 S. aureus isolates, 13 displays more pronounced biofilm
forming ability than the reference strain S. aureus ATCC 11632, and only 4 isolates display lower biofilm forming ability.
The presence of biofilm might be the reason for the recurrence of infection. Therefore, a searching for new
antibiofilm therapeutics in CT therapy is of great importance.",
publisher = "Belgrade: Serbian Society of Microbiology",
journal = "Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia",
title = "Microbiological and histological analysis of tonsil tissue from patients diagnosed with chronic tonsilitis",
pages = "853",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5155"
}

Kostić, M., Ivanov, M., Sanković Babić, S., Tapavčević, Z., Radanović, O., Ćirić, A.,& Soković, M.. (2022). Microbiological and histological analysis of tonsil tissue from patients diagnosed with chronic tonsilitis. in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia
Belgrade: Serbian Society of Microbiology., 853.
https://hdl.handle.net/21.15107/rcub_ibiss_5155

Kostić M, Ivanov M, Sanković Babić S, Tapavčević Z, Radanović O, Ćirić A, Soković M. Microbiological and histological analysis of tonsil tissue from patients diagnosed with chronic tonsilitis. in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia. 2022;:853.
https://hdl.handle.net/21.15107/rcub_ibiss_5155 .

Kostić, Marina, Ivanov, Marija, Sanković Babić, Snežana, Tapavčević, Zvezdana, Radanović, Oliver, Ćirić, Ana, Soković, Marina, "Microbiological and histological analysis of tonsil tissue from patients diagnosed with chronic tonsilitis" in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia (2022):853,
https://hdl.handle.net/21.15107/rcub_ibiss_5155 .

TY  - JOUR
AU  - Carević, Tamara
AU  - Kostić, Marina
AU  - Nikolić, Biljana
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Ivanov, Marija
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5146
AB  - Hesperetin is the aglycone of citrus flavonoid hesperidin. Due to the limited information regarding hesperetin antimicrobial potential and emerging need for novel antimicrobials, we have studied its antimicrobial activity (microdilution assay), antibiofilm activity with different assays in two models (mono- and polymicrobial biofilm), and toxicity (MTT and brine shrimp lethality assays). Hesperetin inhibited growth of all Candida isolates (minimal inhibitory concentration, MIC, 0.165 mg/mL), while it’s inhibitory potential towards Staphylococcus aureus was lower (MIC 4 mg/mL). Hesperetin (0.165 mg/mL) reduced ability of Candida to form biofilms and moderately reduced exopolysaccharide levels in biofilm matrix. Effect on the eradication of 24 h old C. albicans biofilms was promising at 1.320 mg/mL. Inhibition of staphylococcal biofilm formation required higher concentrations of hesperetin (<50% inhibition with MIC 4 mg/mL). Establishment of polymicrobial C. albicans-S. aureus biofilm was significantly inhibited with the lowest examined hesperetin concentration (1 mg/mL) in crystal violet and CFU assays. Hesperetin toxicity was examined towards MRC-5 fibroblasts (IC50 0.340 mg/mL) and in brine shrimp lethality assay (LC50 > 1 mg/mL). Hesperetin is efficient in combating growth and biofilm formation of Candida species. However, its antibacterial application should be further examined due to the cytotoxic effects provoked in the antibacterial concentrations
PB  - Basel: MDPI
T2  - Molecules
T1  - Hesperetin—Between the Ability to Diminish Mono- and Polymicrobial Biofilms and Toxicity
IS  - 20
VL  - 27
DO  - 10.3390/molecules27206806
SP  - 6806
ER  - 

@article{
author = "Carević, Tamara and Kostić, Marina and Nikolić, Biljana and Stojković, Dejan and Soković, Marina and Ivanov, Marija",
year = "2022",
abstract = "Hesperetin is the aglycone of citrus flavonoid hesperidin. Due to the limited information regarding hesperetin antimicrobial potential and emerging need for novel antimicrobials, we have studied its antimicrobial activity (microdilution assay), antibiofilm activity with different assays in two models (mono- and polymicrobial biofilm), and toxicity (MTT and brine shrimp lethality assays). Hesperetin inhibited growth of all Candida isolates (minimal inhibitory concentration, MIC, 0.165 mg/mL), while it’s inhibitory potential towards Staphylococcus aureus was lower (MIC 4 mg/mL). Hesperetin (0.165 mg/mL) reduced ability of Candida to form biofilms and moderately reduced exopolysaccharide levels in biofilm matrix. Effect on the eradication of 24 h old C. albicans biofilms was promising at 1.320 mg/mL. Inhibition of staphylococcal biofilm formation required higher concentrations of hesperetin (<50% inhibition with MIC 4 mg/mL). Establishment of polymicrobial C. albicans-S. aureus biofilm was significantly inhibited with the lowest examined hesperetin concentration (1 mg/mL) in crystal violet and CFU assays. Hesperetin toxicity was examined towards MRC-5 fibroblasts (IC50 0.340 mg/mL) and in brine shrimp lethality assay (LC50 > 1 mg/mL). Hesperetin is efficient in combating growth and biofilm formation of Candida species. However, its antibacterial application should be further examined due to the cytotoxic effects provoked in the antibacterial concentrations",
publisher = "Basel: MDPI",
journal = "Molecules",
title = "Hesperetin—Between the Ability to Diminish Mono- and Polymicrobial Biofilms and Toxicity",
number = "20",
volume = "27",
doi = "10.3390/molecules27206806",
pages = "6806"
}

Carević, T., Kostić, M., Nikolić, B., Stojković, D., Soković, M.,& Ivanov, M.. (2022). Hesperetin—Between the Ability to Diminish Mono- and Polymicrobial Biofilms and Toxicity. in Molecules
Basel: MDPI., 27(20), 6806.
https://doi.org/10.3390/molecules27206806

Carević T, Kostić M, Nikolić B, Stojković D, Soković M, Ivanov M. Hesperetin—Between the Ability to Diminish Mono- and Polymicrobial Biofilms and Toxicity. in Molecules. 2022;27(20):6806.
doi:10.3390/molecules27206806 .

Carević, Tamara, Kostić, Marina, Nikolić, Biljana, Stojković, Dejan, Soković, Marina, Ivanov, Marija, "Hesperetin—Between the Ability to Diminish Mono- and Polymicrobial Biofilms and Toxicity" in Molecules, 27, no. 20 (2022):6806,
https://doi.org/10.3390/molecules27206806 . .

TY  - JOUR
AU  - Ivanov, Marija
AU  - Kostić, Marina
AU  - Stojković, Dejan
AU  - Soković, Marina
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S025462992100497X
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4745
AB  - Emerging antimicrobial resistance and side effects associated with antibiotics overuse are driving the need for the novel antimicrobial therapeutics with the natural compounds considered as an appealing alternative. Rosmarinic acid is abundantly present in medicinal plants. This study aimed to elucidate its possible role as an inhibitor of planktonic and biofilm microorganism growth, along with its antifungal mechanisms. In this study, rosmarinic acid showed promising anticandidal (MIC 0.1–0.2 mg/mL) and antibacterial (MIC 0.002->0.8 mg/mL) activity. To some extent it was able to prevent cell attachment and eradicate established biofilms. Rosmarinic acid antifungal mechanisms included reduction of mitochondrial activity, alteration in membrane integrity, and slight inhibition of protease production but not binding to ergosterol. Its antibiofilm activity was moderately linked to the reduction in exopolysaccharide production. Considering its wide antimicrobial and antibiofilm spectrum rosmarinic acid could be further explored as an antimicrobial agent along with range of medicinal plants with rosmarinic acid as the dominant compound. Nevertheless, rosmarinic acid could serve as the basis for the development of antimicrobials employing novel mechanisms of activity.
T2  - South African Journal of Botany
T1  - Rosmarinic acid–Modes of antimicrobial and antibiofilm activities of common plant polyphenol
VL  - 146
DO  - 10.1016/j.sajb.2021.11.050
SP  - 521
EP  - 527
ER  - 

@article{
author = "Ivanov, Marija and Kostić, Marina and Stojković, Dejan and Soković, Marina",
year = "2022",
abstract = "Emerging antimicrobial resistance and side effects associated with antibiotics overuse are driving the need for the novel antimicrobial therapeutics with the natural compounds considered as an appealing alternative. Rosmarinic acid is abundantly present in medicinal plants. This study aimed to elucidate its possible role as an inhibitor of planktonic and biofilm microorganism growth, along with its antifungal mechanisms. In this study, rosmarinic acid showed promising anticandidal (MIC 0.1–0.2 mg/mL) and antibacterial (MIC 0.002->0.8 mg/mL) activity. To some extent it was able to prevent cell attachment and eradicate established biofilms. Rosmarinic acid antifungal mechanisms included reduction of mitochondrial activity, alteration in membrane integrity, and slight inhibition of protease production but not binding to ergosterol. Its antibiofilm activity was moderately linked to the reduction in exopolysaccharide production. Considering its wide antimicrobial and antibiofilm spectrum rosmarinic acid could be further explored as an antimicrobial agent along with range of medicinal plants with rosmarinic acid as the dominant compound. Nevertheless, rosmarinic acid could serve as the basis for the development of antimicrobials employing novel mechanisms of activity.",
journal = "South African Journal of Botany",
title = "Rosmarinic acid–Modes of antimicrobial and antibiofilm activities of common plant polyphenol",
volume = "146",
doi = "10.1016/j.sajb.2021.11.050",
pages = "521-527"
}

Ivanov, M., Kostić, M., Stojković, D.,& Soković, M.. (2022). Rosmarinic acid–Modes of antimicrobial and antibiofilm activities of common plant polyphenol. in South African Journal of Botany, 146, 521-527.
https://doi.org/10.1016/j.sajb.2021.11.050

Ivanov M, Kostić M, Stojković D, Soković M. Rosmarinic acid–Modes of antimicrobial and antibiofilm activities of common plant polyphenol. in South African Journal of Botany. 2022;146:521-527.
doi:10.1016/j.sajb.2021.11.050 .

Ivanov, Marija, Kostić, Marina, Stojković, Dejan, Soković, Marina, "Rosmarinic acid–Modes of antimicrobial and antibiofilm activities of common plant polyphenol" in South African Journal of Botany, 146 (2022):521-527,
https://doi.org/10.1016/j.sajb.2021.11.050 . .

TY  - JOUR
AU  - Mandim, Filipa
AU  - Petropoulos, Spyridon A.
AU  - Pinela, José
AU  - Dias, Maria Inês
AU  - Kostić, Marina
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Santos-Buelga, Celestino
AU  - Barros, Lillian
PY  - 2022
UR  - https://www.mdpi.com/2079-7737/11/5/699
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4980
AB  - Cardoon (Cynara cardunculus var. altilis) blades were collected at sixteen sampling dates (B1-B16) to study the influence of the phenological growth stage on the phenolic composition and biological properties. Twenty phenolic compounds were identified, among which trans 3,4-O-dicaffeoylquinic acid, 5-O-caffeoylquinic acid, and luteolin-O-hexoside (39.6, 42.6, and 101.0 mg/g extract, respectively) were the main compounds. Immature blades (B3) had a higher content of phenolic compounds (178 mg/g extract) and a greater ability to inhibit the formation of thiobarbituric acid reactive substances (IC50 of 1.61 µg/mL). Samples at more advanced growth stages revealed a greater capacity to inhibit oxidative hemolysis (B8, IC50 of 25 and 47.4 µg/mL for Δt of 60 and 120 min, respectively) and higher cytotoxic (B8-B13, GI50 between 7.1 and 17 µg/mL), anti-inflammatory (B13, IC50 of 10 µg/mL), and antibacterial activities. In turn, the antifungal activity varied depending on the tested fungi. All these results suggest that maturity influences the phenolic composition and bioactive properties of cardoon blades, which reveal great potential for the development of bioactive ingredients for food and pharmaceutical applications, among others.
PB  - Basel: MDPI
T2  - Biology
T1  - Phenolic Composition and Antioxidant, Anti-Inflammatory, Cytotoxic, and Antimicrobial Activities of Cardoon Blades at Different Growth Stages.
IS  - 5
VL  - 11
DO  - 10.3390/biology11050699
SP  - 699
ER  - 

@article{
author = "Mandim, Filipa and Petropoulos, Spyridon A. and Pinela, José and Dias, Maria Inês and Kostić, Marina and Soković, Marina and Ferreira, Isabel C. F. R. and Santos-Buelga, Celestino and Barros, Lillian",
year = "2022",
abstract = "Cardoon (Cynara cardunculus var. altilis) blades were collected at sixteen sampling dates (B1-B16) to study the influence of the phenological growth stage on the phenolic composition and biological properties. Twenty phenolic compounds were identified, among which trans 3,4-O-dicaffeoylquinic acid, 5-O-caffeoylquinic acid, and luteolin-O-hexoside (39.6, 42.6, and 101.0 mg/g extract, respectively) were the main compounds. Immature blades (B3) had a higher content of phenolic compounds (178 mg/g extract) and a greater ability to inhibit the formation of thiobarbituric acid reactive substances (IC50 of 1.61 µg/mL). Samples at more advanced growth stages revealed a greater capacity to inhibit oxidative hemolysis (B8, IC50 of 25 and 47.4 µg/mL for Δt of 60 and 120 min, respectively) and higher cytotoxic (B8-B13, GI50 between 7.1 and 17 µg/mL), anti-inflammatory (B13, IC50 of 10 µg/mL), and antibacterial activities. In turn, the antifungal activity varied depending on the tested fungi. All these results suggest that maturity influences the phenolic composition and bioactive properties of cardoon blades, which reveal great potential for the development of bioactive ingredients for food and pharmaceutical applications, among others.",
publisher = "Basel: MDPI",
journal = "Biology",
title = "Phenolic Composition and Antioxidant, Anti-Inflammatory, Cytotoxic, and Antimicrobial Activities of Cardoon Blades at Different Growth Stages.",
number = "5",
volume = "11",
doi = "10.3390/biology11050699",
pages = "699"
}

Mandim, F., Petropoulos, S. A., Pinela, J., Dias, M. I., Kostić, M., Soković, M., Ferreira, I. C. F. R., Santos-Buelga, C.,& Barros, L.. (2022). Phenolic Composition and Antioxidant, Anti-Inflammatory, Cytotoxic, and Antimicrobial Activities of Cardoon Blades at Different Growth Stages.. in Biology
Basel: MDPI., 11(5), 699.
https://doi.org/10.3390/biology11050699

Mandim F, Petropoulos SA, Pinela J, Dias MI, Kostić M, Soković M, Ferreira ICFR, Santos-Buelga C, Barros L. Phenolic Composition and Antioxidant, Anti-Inflammatory, Cytotoxic, and Antimicrobial Activities of Cardoon Blades at Different Growth Stages.. in Biology. 2022;11(5):699.
doi:10.3390/biology11050699 .

Mandim, Filipa, Petropoulos, Spyridon A., Pinela, José, Dias, Maria Inês, Kostić, Marina, Soković, Marina, Ferreira, Isabel C. F. R., Santos-Buelga, Celestino, Barros, Lillian, "Phenolic Composition and Antioxidant, Anti-Inflammatory, Cytotoxic, and Antimicrobial Activities of Cardoon Blades at Different Growth Stages." in Biology, 11, no. 5 (2022):699,
https://doi.org/10.3390/biology11050699 . .

TY  - JOUR
AU  - Silva, Ana Rita
AU  - Ayuso, Manuel
AU  - Pereira, Carla
AU  - Dias, Maria Inês
AU  - Kostić, Marina
AU  - Calhelha, Ricardo C.
AU  - Soković, Marina
AU  - García, Pablo A.
AU  - Ferreira, Isabel C.F.R.
AU  - Barros, Lillian
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0926669021011080
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4746
AB  - Cytinus hypocistis (L.) L. is a comestible holoparasite with great potential for cosmeceutical application. Although its high tannin content has been associated with its bioactive and inhibitory enzyme properties, this is the first report establishing a relationship between parasite and host (Halimium lasianthum (Lam.) Greuter) phenolic profile and bioactive properties. Thus, five extracts (aerial and root extracts of non-parasited and parasited H. lasianthum and C. hypocistis) were evaluated. The tentative identification of both species comprises 39 phenolic compounds. Hydrolysable tannins and flavonoids were the main identified groups in C. hypocistis and H. lasianthum extracts, respectively. Regarding bioactivities, C. hypocistis exhibited excellent antioxidant results both in Oxidative Haemolysis (OxHLIA) and inhibition of Thiobarbituric Acid Reactive Substances Formation (TBARS). The tested extracts presented antimicrobial inhibition, anti-inflammatory activity, and effective cytotoxicity against tumour cells. C. hypocistis exhibited the lowest cytotoxicity on a non-tumour cell line. Principal Component Analysis (PCA) was a suitable approach to analyse differences among samples, explaining up to 67% of data variability and suggesting no similarities between parasite and host phenolic composition and bioactivities. Therefore, this comparative study emphasises the significance of both species as a source of biologically active compounds.
PB  - Amsterdam: Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Evaluation of parasite and host phenolic composition and bioactivities − The Practical Case of Cytinus hypocistis (L.) L. and Halimium lasianthum (Lam.) Greuter
VL  - 176
DO  - 10.1016/j.indcrop.2021.114343
SP  - 114343
ER  - 

@article{
author = "Silva, Ana Rita and Ayuso, Manuel and Pereira, Carla and Dias, Maria Inês and Kostić, Marina and Calhelha, Ricardo C. and Soković, Marina and García, Pablo A. and Ferreira, Isabel C.F.R. and Barros, Lillian",
year = "2022",
abstract = "Cytinus hypocistis (L.) L. is a comestible holoparasite with great potential for cosmeceutical application. Although its high tannin content has been associated with its bioactive and inhibitory enzyme properties, this is the first report establishing a relationship between parasite and host (Halimium lasianthum (Lam.) Greuter) phenolic profile and bioactive properties. Thus, five extracts (aerial and root extracts of non-parasited and parasited H. lasianthum and C. hypocistis) were evaluated. The tentative identification of both species comprises 39 phenolic compounds. Hydrolysable tannins and flavonoids were the main identified groups in C. hypocistis and H. lasianthum extracts, respectively. Regarding bioactivities, C. hypocistis exhibited excellent antioxidant results both in Oxidative Haemolysis (OxHLIA) and inhibition of Thiobarbituric Acid Reactive Substances Formation (TBARS). The tested extracts presented antimicrobial inhibition, anti-inflammatory activity, and effective cytotoxicity against tumour cells. C. hypocistis exhibited the lowest cytotoxicity on a non-tumour cell line. Principal Component Analysis (PCA) was a suitable approach to analyse differences among samples, explaining up to 67% of data variability and suggesting no similarities between parasite and host phenolic composition and bioactivities. Therefore, this comparative study emphasises the significance of both species as a source of biologically active compounds.",
publisher = "Amsterdam: Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Evaluation of parasite and host phenolic composition and bioactivities − The Practical Case of Cytinus hypocistis (L.) L. and Halimium lasianthum (Lam.) Greuter",
volume = "176",
doi = "10.1016/j.indcrop.2021.114343",
pages = "114343"
}

Silva, A. R., Ayuso, M., Pereira, C., Dias, M. I., Kostić, M., Calhelha, R. C., Soković, M., García, P. A., Ferreira, I. C.F.R.,& Barros, L.. (2022). Evaluation of parasite and host phenolic composition and bioactivities − The Practical Case of Cytinus hypocistis (L.) L. and Halimium lasianthum (Lam.) Greuter. in Industrial Crops and Products
Amsterdam: Elsevier B.V.., 176, 114343.
https://doi.org/10.1016/j.indcrop.2021.114343

Silva AR, Ayuso M, Pereira C, Dias MI, Kostić M, Calhelha RC, Soković M, García PA, Ferreira IC, Barros L. Evaluation of parasite and host phenolic composition and bioactivities − The Practical Case of Cytinus hypocistis (L.) L. and Halimium lasianthum (Lam.) Greuter. in Industrial Crops and Products. 2022;176:114343.
doi:10.1016/j.indcrop.2021.114343 .

Silva, Ana Rita, Ayuso, Manuel, Pereira, Carla, Dias, Maria Inês, Kostić, Marina, Calhelha, Ricardo C., Soković, Marina, García, Pablo A., Ferreira, Isabel C.F.R., Barros, Lillian, "Evaluation of parasite and host phenolic composition and bioactivities − The Practical Case of Cytinus hypocistis (L.) L. and Halimium lasianthum (Lam.) Greuter" in Industrial Crops and Products, 176 (2022):114343,
https://doi.org/10.1016/j.indcrop.2021.114343 . .

TY  - CONF
AU  - Stojković, Dejan
AU  - Kostić, Marina
AU  - Fernandes, Angela
AU  - Barros, Lillian
AU  - Glamočlija, Jasmina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5157
AB  - Craterellus cornucopioides (L.) Pers. (Cantharellaceae), or horn of plenty, is an edible fungi. It is also known as the black chanterelle, black trumpet, crna truba (Serbian), trompette de la mort (French), trombetta dei morti (Italian) or trumpet of the dead, djondjon (Haitian). C. cornucopioides growing wild in Serbia was chemically characterized in nutritional value and individual compounds (sugars, fatty acids, tocopherols, organic acids and phenolic compounds). Antioxidant (scavenging activity, reducing power and inhibition of lipid peroxidation), antibacterial and antifungal activities of its methanolic extract were evaluated under in vitro conditions. C. cornucopioides was showed to be rich in carbohydrates (79.32 g/100g dw), followed by proteins (8.36 g/100g dw), ash (9.43 g/100g dw) and fat (2.90 g/100g dw). The most abundant sugar compound was identified as mannitol (14.16 g/100g dw); four tocopherol isoforms (α-, β-,γ- and δ-tocopherols) were detected. Unsaturated fatty acids predominated over saturated fatty acids, with oleic and linoleic acids as the most dominant ones. Five organic acids were quantified: oxalic, citric, quinic, and fumaric and malic acids. The methanolic extract of the mushroom showed in vitro antioxidant and antimicrobial activities. Methanolic extract successively inhibited growth of pathogenic bacteria.
PB  - Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia
C3  - Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia
T1  - Nutritional properties, chemical composition and biological properties of Craterellus cornucopioides
SP  - 168
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5157
ER  - 

@conference{
author = "Stojković, Dejan and Kostić, Marina and Fernandes, Angela and Barros, Lillian and Glamočlija, Jasmina",
year = "2022",
abstract = "Craterellus cornucopioides (L.) Pers. (Cantharellaceae), or horn of plenty, is an edible fungi. It is also known as the black chanterelle, black trumpet, crna truba (Serbian), trompette de la mort (French), trombetta dei morti (Italian) or trumpet of the dead, djondjon (Haitian). C. cornucopioides growing wild in Serbia was chemically characterized in nutritional value and individual compounds (sugars, fatty acids, tocopherols, organic acids and phenolic compounds). Antioxidant (scavenging activity, reducing power and inhibition of lipid peroxidation), antibacterial and antifungal activities of its methanolic extract were evaluated under in vitro conditions. C. cornucopioides was showed to be rich in carbohydrates (79.32 g/100g dw), followed by proteins (8.36 g/100g dw), ash (9.43 g/100g dw) and fat (2.90 g/100g dw). The most abundant sugar compound was identified as mannitol (14.16 g/100g dw); four tocopherol isoforms (α-, β-,γ- and δ-tocopherols) were detected. Unsaturated fatty acids predominated over saturated fatty acids, with oleic and linoleic acids as the most dominant ones. Five organic acids were quantified: oxalic, citric, quinic, and fumaric and malic acids. The methanolic extract of the mushroom showed in vitro antioxidant and antimicrobial activities. Methanolic extract successively inhibited growth of pathogenic bacteria.",
publisher = "Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia",
journal = "Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia",
title = "Nutritional properties, chemical composition and biological properties of Craterellus cornucopioides",
pages = "168",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5157"
}

Stojković, D., Kostić, M., Fernandes, A., Barros, L.,& Glamočlija, J.. (2022). Nutritional properties, chemical composition and biological properties of Craterellus cornucopioides. in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia
Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia., 168.
https://hdl.handle.net/21.15107/rcub_ibiss_5157

Stojković D, Kostić M, Fernandes A, Barros L, Glamočlija J. Nutritional properties, chemical composition and biological properties of Craterellus cornucopioides. in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia. 2022;:168.
https://hdl.handle.net/21.15107/rcub_ibiss_5157 .

Stojković, Dejan, Kostić, Marina, Fernandes, Angela, Barros, Lillian, Glamočlija, Jasmina, "Nutritional properties, chemical composition and biological properties of Craterellus cornucopioides" in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia (2022):168,
https://hdl.handle.net/21.15107/rcub_ibiss_5157 .

TY  - JOUR
AU  - Čutović, Natalija
AU  - Marković, Tatjana
AU  - Kostić, Marina
AU  - Gašić, Uroš
AU  - Prijić, Željana
AU  - Ren, Xiuxia
AU  - Lukić, Milan
AU  - Bugarski, Branko
PY  - 2022
UR  - https://www.mdpi.com/1424-8247/15/12/1537
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5358
AB  - Without being aware of its chemical makeup, many ancient societies have used Steppe peony in their traditional medicine. Given that modern phytopreparation intended for use on human skin requires, above all, knowledge of its chemical composition, the goal of this study was to make a screening of the composition of aqueous and methanolic extracts of the petals of P. tenuifolia L. and to examine them for various skin-beneficial properties. The extracts were prepared by maceration, ultrasound-assisted, and microwave-assisted extraction procedures. The chemical profiling was conducted by the use of UHPLC-LTQ-OrbiTrap MS and UHPLC/MS, and spectrophotometric methods for the determination of total polyphenol and total flavonoid contents. The biological activities entailed antioxidant ABTS, DPPH, CUPRAC (Cupric Ion Reducing Antioxidant Capacity), and FRAP (Ferric Reducing Antioxidant Power) assays, antimicrobial (antibacterial and antifungal) and antibiofilm activities, cytotoxicity, wound healing potential, as well as the adhesion and invasion of Staphylococcus lugdunensis. The results showed that the petals are rich in phenolic acids and flavonoids, which are commonly associated with numerous biological activities. The aqueous extracts were more efficient in the majority of the bioactivity assays then the methanolic ones, whereas the optimal extraction method varied between the assays. This study is the first step towards the safe use of the aqueous extracts of P. tenuifolia petals for therapeutic skin treatments.
PB  - Basel: MDPI
T2  - Pharmaceuticals
T1  - Chemical Profile and Skin-Beneficial Activities of the Petal Extracts of Paeonia tenuifolia L. from Serbia
IS  - 12
VL  - 15
DO  - 10.3390/ph15121537
SP  - 1537
ER  - 

@article{
author = "Čutović, Natalija and Marković, Tatjana and Kostić, Marina and Gašić, Uroš and Prijić, Željana and Ren, Xiuxia and Lukić, Milan and Bugarski, Branko",
year = "2022",
abstract = "Without being aware of its chemical makeup, many ancient societies have used Steppe peony in their traditional medicine. Given that modern phytopreparation intended for use on human skin requires, above all, knowledge of its chemical composition, the goal of this study was to make a screening of the composition of aqueous and methanolic extracts of the petals of P. tenuifolia L. and to examine them for various skin-beneficial properties. The extracts were prepared by maceration, ultrasound-assisted, and microwave-assisted extraction procedures. The chemical profiling was conducted by the use of UHPLC-LTQ-OrbiTrap MS and UHPLC/MS, and spectrophotometric methods for the determination of total polyphenol and total flavonoid contents. The biological activities entailed antioxidant ABTS, DPPH, CUPRAC (Cupric Ion Reducing Antioxidant Capacity), and FRAP (Ferric Reducing Antioxidant Power) assays, antimicrobial (antibacterial and antifungal) and antibiofilm activities, cytotoxicity, wound healing potential, as well as the adhesion and invasion of Staphylococcus lugdunensis. The results showed that the petals are rich in phenolic acids and flavonoids, which are commonly associated with numerous biological activities. The aqueous extracts were more efficient in the majority of the bioactivity assays then the methanolic ones, whereas the optimal extraction method varied between the assays. This study is the first step towards the safe use of the aqueous extracts of P. tenuifolia petals for therapeutic skin treatments.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals",
title = "Chemical Profile and Skin-Beneficial Activities of the Petal Extracts of Paeonia tenuifolia L. from Serbia",
number = "12",
volume = "15",
doi = "10.3390/ph15121537",
pages = "1537"
}

Čutović, N., Marković, T., Kostić, M., Gašić, U., Prijić, Ž., Ren, X., Lukić, M.,& Bugarski, B.. (2022). Chemical Profile and Skin-Beneficial Activities of the Petal Extracts of Paeonia tenuifolia L. from Serbia. in Pharmaceuticals
Basel: MDPI., 15(12), 1537.
https://doi.org/10.3390/ph15121537

Čutović N, Marković T, Kostić M, Gašić U, Prijić Ž, Ren X, Lukić M, Bugarski B. Chemical Profile and Skin-Beneficial Activities of the Petal Extracts of Paeonia tenuifolia L. from Serbia. in Pharmaceuticals. 2022;15(12):1537.
doi:10.3390/ph15121537 .

Čutović, Natalija, Marković, Tatjana, Kostić, Marina, Gašić, Uroš, Prijić, Željana, Ren, Xiuxia, Lukić, Milan, Bugarski, Branko, "Chemical Profile and Skin-Beneficial Activities of the Petal Extracts of Paeonia tenuifolia L. from Serbia" in Pharmaceuticals, 15, no. 12 (2022):1537,
https://doi.org/10.3390/ph15121537 . .

TY  - JOUR
AU  - Horishny, Volodymyr
AU  - Geronikaki, Athina
AU  - Kartsev, Victor
AU  - Matiychuk, Vasyl
AU  - Petrou, Anthi
AU  - Pogodin, Pavel
AU  - Poroikov, Vladimir
AU  - Papadopoulou, Theodora A
AU  - Vizirianakis, Ioannis S
AU  - Kostić, Marina
AU  - Ivanov, Marija
AU  - Soković, Marina
PY  - 2022
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8839324
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4884
AB  - BACKGROUND Infectious diseases represent a significant global strain on public health security and impact on socio-economic stability all over the world. The increasing resistance to the current antimicrobial treatment has resulted in the crucial need for the discovery and development of novel entities for the infectious treatment with different modes of action that could target both sensitive and resistant strains. METHODS Compounds were synthesized using the classical organic chemistry methods. Prediction of biological activity spectra was carried out using PASS and PASS-based web applications. Pharmacophore modeling in LigandScout software was used for quantitative modeling of the antibacterial activity. Antimicrobial activity was evaluated using the microdilution method. AutoDock 4.2® software was used to elucidate probable bacterial and fungal molecular targets of the studied compounds. RESULTS All compounds exhibited better antibacterial potency than ampicillin against all bacteria tested. Three compounds were tested against resistant strains MRSA, P. aeruginosa and E. coli and were found to be more potent than MRSA than reference drugs. All compounds demonstrated a higher degree of antifungal activity than the reference drugs bifonazole (6-17-fold) and ketoconazole (13-52-fold). Three of the most active compounds could be considered for further development of the new, more potent antimicrobial agents. CONCLUSION Compounds 5b (Z)-3-(3-hydroxyphenyl)-5-((1-methyl-1H-indol-3-yl)methylene)-2-thioxothiazolidin-4-one and 5g (Z)-3-[5-(1H-Indol-3-ylmethylene)-4-oxo-2-thioxo-thiazolidin-3-yl]-benzoic acid as well as 5h (Z)-3-(5-((5-methoxy-1H-indol-3-yl)methylene)-4-oxo-2-thioxothiazolidin-3-yl)benzoic acid can be considered as lead compounds for further development of more potent and safe antibacterial and antifungal agents.
PB  - Basel: MDPI
T2  - Molecules
T1  - Synthesis, Biological Evaluation and Molecular Docking Studies of 5-Indolylmethylen-4-oxo-2-thioxothiazolidine Derivatives.
IS  - 3
VL  - 27
DO  - 10.3390/molecules27031068
SP  - 1068
ER  - 

@article{
author = "Horishny, Volodymyr and Geronikaki, Athina and Kartsev, Victor and Matiychuk, Vasyl and Petrou, Anthi and Pogodin, Pavel and Poroikov, Vladimir and Papadopoulou, Theodora A and Vizirianakis, Ioannis S and Kostić, Marina and Ivanov, Marija and Soković, Marina",
year = "2022",
abstract = "BACKGROUND Infectious diseases represent a significant global strain on public health security and impact on socio-economic stability all over the world. The increasing resistance to the current antimicrobial treatment has resulted in the crucial need for the discovery and development of novel entities for the infectious treatment with different modes of action that could target both sensitive and resistant strains. METHODS Compounds were synthesized using the classical organic chemistry methods. Prediction of biological activity spectra was carried out using PASS and PASS-based web applications. Pharmacophore modeling in LigandScout software was used for quantitative modeling of the antibacterial activity. Antimicrobial activity was evaluated using the microdilution method. AutoDock 4.2® software was used to elucidate probable bacterial and fungal molecular targets of the studied compounds. RESULTS All compounds exhibited better antibacterial potency than ampicillin against all bacteria tested. Three compounds were tested against resistant strains MRSA, P. aeruginosa and E. coli and were found to be more potent than MRSA than reference drugs. All compounds demonstrated a higher degree of antifungal activity than the reference drugs bifonazole (6-17-fold) and ketoconazole (13-52-fold). Three of the most active compounds could be considered for further development of the new, more potent antimicrobial agents. CONCLUSION Compounds 5b (Z)-3-(3-hydroxyphenyl)-5-((1-methyl-1H-indol-3-yl)methylene)-2-thioxothiazolidin-4-one and 5g (Z)-3-[5-(1H-Indol-3-ylmethylene)-4-oxo-2-thioxo-thiazolidin-3-yl]-benzoic acid as well as 5h (Z)-3-(5-((5-methoxy-1H-indol-3-yl)methylene)-4-oxo-2-thioxothiazolidin-3-yl)benzoic acid can be considered as lead compounds for further development of more potent and safe antibacterial and antifungal agents.",
publisher = "Basel: MDPI",
journal = "Molecules",
title = "Synthesis, Biological Evaluation and Molecular Docking Studies of 5-Indolylmethylen-4-oxo-2-thioxothiazolidine Derivatives.",
number = "3",
volume = "27",
doi = "10.3390/molecules27031068",
pages = "1068"
}

Horishny, V., Geronikaki, A., Kartsev, V., Matiychuk, V., Petrou, A., Pogodin, P., Poroikov, V., Papadopoulou, T. A., Vizirianakis, I. S., Kostić, M., Ivanov, M.,& Soković, M.. (2022). Synthesis, Biological Evaluation and Molecular Docking Studies of 5-Indolylmethylen-4-oxo-2-thioxothiazolidine Derivatives.. in Molecules
Basel: MDPI., 27(3), 1068.
https://doi.org/10.3390/molecules27031068

Horishny V, Geronikaki A, Kartsev V, Matiychuk V, Petrou A, Pogodin P, Poroikov V, Papadopoulou TA, Vizirianakis IS, Kostić M, Ivanov M, Soković M. Synthesis, Biological Evaluation and Molecular Docking Studies of 5-Indolylmethylen-4-oxo-2-thioxothiazolidine Derivatives.. in Molecules. 2022;27(3):1068.
doi:10.3390/molecules27031068 .

Horishny, Volodymyr, Geronikaki, Athina, Kartsev, Victor, Matiychuk, Vasyl, Petrou, Anthi, Pogodin, Pavel, Poroikov, Vladimir, Papadopoulou, Theodora A, Vizirianakis, Ioannis S, Kostić, Marina, Ivanov, Marija, Soković, Marina, "Synthesis, Biological Evaluation and Molecular Docking Studies of 5-Indolylmethylen-4-oxo-2-thioxothiazolidine Derivatives." in Molecules, 27, no. 3 (2022):1068,
https://doi.org/10.3390/molecules27031068 . .

TY  - JOUR
AU  - Petrović, Jovana
AU  - Kovalenko, Vitaly
AU  - Svirid, Anastasia
AU  - Stojković, Dejan
AU  - Ivanov, Marija
AU  - Kostić, Marina
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0022286021021190
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4744
AB  - Naturally occurring terpene core compounds have been used extensively in both pharmaceutical and cosmetic industry. However, since chirality of these compounds has profound influence on the level of their bioactivity, the aim of the present study was to assess broad spectrum activities of four possible stereoisomers of monoterpene alcohol verbenol and both enantiomers of monoterpene ketone verbenone. Antimicrobial activity was evaluated against different bacterial and fungal strains of clinical relevance. Tested compounds exhibited antibacterial activity within MICs range of 0.6–2.5 mg mL−1, antifungal activity within MICs range of 0.08–0.6 mg mL−1, and anticandidal activity with MICs 0.3–1.2 mg mL−1. While there was no difference in antibacterial activity between the enantiomers of verbenone, this was not the case with tested verbenol enantiomers. Furthermore, the compounds expressed no cytotoxicity to human keratinocyte cell line HaCaT at the tested concentrations. Interestingly, compounds contributed to the downregulation of interleukin IL-6 levels in HaCaT cells exposed to bacterial agent Staphylococcus lugdunensis. According to our knowledge, this study is the first that comparatively describes biological activities of stereoisomers of verbenol and enantiomers of verbenone. Current findings highlighted the importance of stereochemical purity of monoterpenoids in relation to their unique biological features.
T2  - Journal of Molecular Structure
T1  - Individual stereoisomers of verbenol and verbenone express bioactive features
VL  - 1251
DO  - 10.1016/j.molstruc.2021.131999
SP  - 131999
ER  - 

@article{
author = "Petrović, Jovana and Kovalenko, Vitaly and Svirid, Anastasia and Stojković, Dejan and Ivanov, Marija and Kostić, Marina",
year = "2022",
abstract = "Naturally occurring terpene core compounds have been used extensively in both pharmaceutical and cosmetic industry. However, since chirality of these compounds has profound influence on the level of their bioactivity, the aim of the present study was to assess broad spectrum activities of four possible stereoisomers of monoterpene alcohol verbenol and both enantiomers of monoterpene ketone verbenone. Antimicrobial activity was evaluated against different bacterial and fungal strains of clinical relevance. Tested compounds exhibited antibacterial activity within MICs range of 0.6–2.5 mg mL−1, antifungal activity within MICs range of 0.08–0.6 mg mL−1, and anticandidal activity with MICs 0.3–1.2 mg mL−1. While there was no difference in antibacterial activity between the enantiomers of verbenone, this was not the case with tested verbenol enantiomers. Furthermore, the compounds expressed no cytotoxicity to human keratinocyte cell line HaCaT at the tested concentrations. Interestingly, compounds contributed to the downregulation of interleukin IL-6 levels in HaCaT cells exposed to bacterial agent Staphylococcus lugdunensis. According to our knowledge, this study is the first that comparatively describes biological activities of stereoisomers of verbenol and enantiomers of verbenone. Current findings highlighted the importance of stereochemical purity of monoterpenoids in relation to their unique biological features.",
journal = "Journal of Molecular Structure",
title = "Individual stereoisomers of verbenol and verbenone express bioactive features",
volume = "1251",
doi = "10.1016/j.molstruc.2021.131999",
pages = "131999"
}

Petrović, J., Kovalenko, V., Svirid, A., Stojković, D., Ivanov, M.,& Kostić, M.. (2022). Individual stereoisomers of verbenol and verbenone express bioactive features. in Journal of Molecular Structure, 1251, 131999.
https://doi.org/10.1016/j.molstruc.2021.131999

Petrović J, Kovalenko V, Svirid A, Stojković D, Ivanov M, Kostić M. Individual stereoisomers of verbenol and verbenone express bioactive features. in Journal of Molecular Structure. 2022;1251:131999.
doi:10.1016/j.molstruc.2021.131999 .

Petrović, Jovana, Kovalenko, Vitaly, Svirid, Anastasia, Stojković, Dejan, Ivanov, Marija, Kostić, Marina, "Individual stereoisomers of verbenol and verbenone express bioactive features" in Journal of Molecular Structure, 1251 (2022):131999,
https://doi.org/10.1016/j.molstruc.2021.131999 . .

TY  - CHAP
AU  - Petrović, Jovana
AU  - Kostić, Marina
AU  - Stojković, Dejan
AU  - Glamočlija, Jasmina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5808
AB  - Fungi are an untapped resource with both nutritive and medicinal potentials,
that have been undervalued and underexplored until recently, but they are now
increasingly utilized in the pharmaceutical and food industries. With the re evaluated concept of using food in prophylaxis of diseases and as therapeutic
agents, contemporary research has been strongly focused on this area, leading
to scientifically supported facts. Since the nutritive properties of edible
mushrooms have been praised in scientific research, an up-to-date literature
review on these properties will be presented so as to evaluate the novel prospects and possible applications of edible mushrooms in the food industry
PB  - London, UK: The Royal Society of Chemistry
T2  - Edible Fungi: Chemical Composition, Nutrition and Health Effects
T1  - Applications of Mushrooms in the food industry
DO  - 10.1039/9781839167522-00359
SP  - 359
EP  - 382
ER  - 

@inbook{
author = "Petrović, Jovana and Kostić, Marina and Stojković, Dejan and Glamočlija, Jasmina",
year = "2022",
abstract = "Fungi are an untapped resource with both nutritive and medicinal potentials,
that have been undervalued and underexplored until recently, but they are now
increasingly utilized in the pharmaceutical and food industries. With the re evaluated concept of using food in prophylaxis of diseases and as therapeutic
agents, contemporary research has been strongly focused on this area, leading
to scientifically supported facts. Since the nutritive properties of edible
mushrooms have been praised in scientific research, an up-to-date literature
review on these properties will be presented so as to evaluate the novel prospects and possible applications of edible mushrooms in the food industry",
publisher = "London, UK: The Royal Society of Chemistry",
journal = "Edible Fungi: Chemical Composition, Nutrition and Health Effects",
booktitle = "Applications of Mushrooms in the food industry",
doi = "10.1039/9781839167522-00359",
pages = "359-382"
}

Petrović, J., Kostić, M., Stojković, D.,& Glamočlija, J.. (2022). Applications of Mushrooms in the food industry. in Edible Fungi: Chemical Composition, Nutrition and Health Effects
London, UK: The Royal Society of Chemistry., 359-382.
https://doi.org/10.1039/9781839167522-00359

Petrović J, Kostić M, Stojković D, Glamočlija J. Applications of Mushrooms in the food industry. in Edible Fungi: Chemical Composition, Nutrition and Health Effects. 2022;:359-382.
doi:10.1039/9781839167522-00359 .

Petrović, Jovana, Kostić, Marina, Stojković, Dejan, Glamočlija, Jasmina, "Applications of Mushrooms in the food industry" in Edible Fungi: Chemical Composition, Nutrition and Health Effects (2022):359-382,
https://doi.org/10.1039/9781839167522-00359 . .

TY  - CONF
AU  - Ivanov, Marija
AU  - Carević, Tamara
AU  - Kostić, Marina
AU  - Ćirić, Ana
AU  - Petrović, Jovana
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5751
AB  - Terpenoids are the most complex class of natural products with over 80000 identified representatives found in plants, microorganisms, insects, and marine invertebrates. Their anticandidal potential, especially impact on fungal virulence is, however, not fully explored yet. We aimed to investigate anticandidal, antibiofilm, and antihyphal properties of selected terpenoids (borneol, thymol, geraniol, and carvacrol). The strongest antifungal potential towards Candida albicans strains could be noticed for borneol (lowest minimal inhibitory concentration, MIC, 0.06 mg/mL) followed by thymol (lowest MIC 0.12 mg/mL). On the other hand, MIC of geraniol was up to 8 mg/mL. Non-albicans Candida strains were most susceptible to thymol (MIC 0.12 mg/mL towards C. krusei H1/16) and borneol (MIC 0.12 mg/mL towards C. parapsilosis ATCC 22019). When the terpenoids were applied in their previously determined minimal inhibitory concentrations the strongest reducing impact on C. albicans ATCC 10231 biofilm forming ability was observed for carvacrol (60% inhibition), followed by geraniol (56% inhibition). Lower concentration of terpenoids (0.5 MIC) led to similar impairment of biofilm formation – the strongest impact was noticed for geraniol (54% inhibition), followed by carvacrol (47% inhibition) as determined by crystal violet assay. MTT antibiofilm assay highlighted great potential of carvacrol and geraniol to reduce viability of Candida cells in biofilms. Inhibition of hyphal formation could be noticed only upon application of carvacrol (14.29% of hyphal cells compared to the untreated control with 50.67% hyphae) suggesting wide antivirulence potential exhibited by this molecule. Other compounds explored in this study have displayed high MIC values (geraniol), low antibiofilm potential (thymol, borneol) or lack of antihyphal properties (all except carvacrol). Potential of carvacrol to reduce fungal virulence should be further studied on molecular level and in in vivo assays.
PB  - Belgrade: University of Belgrade, Faculty of Agriculture
C3  - Book of abstracts: 1st European Symposium on Phytochemicals in Medicine and Food, 1-EuSPMF; 2022 Sep 7-9; Belgrade, Serbia
T1  - Selected terpenoids as obstructers of Candida virulence
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5751
ER  - 

@conference{
author = "Ivanov, Marija and Carević, Tamara and Kostić, Marina and Ćirić, Ana and Petrović, Jovana and Glamočlija, Jasmina and Soković, Marina",
year = "2022",
abstract = "Terpenoids are the most complex class of natural products with over 80000 identified representatives found in plants, microorganisms, insects, and marine invertebrates. Their anticandidal potential, especially impact on fungal virulence is, however, not fully explored yet. We aimed to investigate anticandidal, antibiofilm, and antihyphal properties of selected terpenoids (borneol, thymol, geraniol, and carvacrol). The strongest antifungal potential towards Candida albicans strains could be noticed for borneol (lowest minimal inhibitory concentration, MIC, 0.06 mg/mL) followed by thymol (lowest MIC 0.12 mg/mL). On the other hand, MIC of geraniol was up to 8 mg/mL. Non-albicans Candida strains were most susceptible to thymol (MIC 0.12 mg/mL towards C. krusei H1/16) and borneol (MIC 0.12 mg/mL towards C. parapsilosis ATCC 22019). When the terpenoids were applied in their previously determined minimal inhibitory concentrations the strongest reducing impact on C. albicans ATCC 10231 biofilm forming ability was observed for carvacrol (60% inhibition), followed by geraniol (56% inhibition). Lower concentration of terpenoids (0.5 MIC) led to similar impairment of biofilm formation – the strongest impact was noticed for geraniol (54% inhibition), followed by carvacrol (47% inhibition) as determined by crystal violet assay. MTT antibiofilm assay highlighted great potential of carvacrol and geraniol to reduce viability of Candida cells in biofilms. Inhibition of hyphal formation could be noticed only upon application of carvacrol (14.29% of hyphal cells compared to the untreated control with 50.67% hyphae) suggesting wide antivirulence potential exhibited by this molecule. Other compounds explored in this study have displayed high MIC values (geraniol), low antibiofilm potential (thymol, borneol) or lack of antihyphal properties (all except carvacrol). Potential of carvacrol to reduce fungal virulence should be further studied on molecular level and in in vivo assays.",
publisher = "Belgrade: University of Belgrade, Faculty of Agriculture",
journal = "Book of abstracts: 1st European Symposium on Phytochemicals in Medicine and Food, 1-EuSPMF; 2022 Sep 7-9; Belgrade, Serbia",
title = "Selected terpenoids as obstructers of Candida virulence",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5751"
}

Ivanov, M., Carević, T., Kostić, M., Ćirić, A., Petrović, J., Glamočlija, J.,& Soković, M.. (2022). Selected terpenoids as obstructers of Candida virulence. in Book of abstracts: 1st European Symposium on Phytochemicals in Medicine and Food, 1-EuSPMF; 2022 Sep 7-9; Belgrade, Serbia
Belgrade: University of Belgrade, Faculty of Agriculture..
https://hdl.handle.net/21.15107/rcub_ibiss_5751

Ivanov M, Carević T, Kostić M, Ćirić A, Petrović J, Glamočlija J, Soković M. Selected terpenoids as obstructers of Candida virulence. in Book of abstracts: 1st European Symposium on Phytochemicals in Medicine and Food, 1-EuSPMF; 2022 Sep 7-9; Belgrade, Serbia. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_5751 .

Ivanov, Marija, Carević, Tamara, Kostić, Marina, Ćirić, Ana, Petrović, Jovana, Glamočlija, Jasmina, Soković, Marina, "Selected terpenoids as obstructers of Candida virulence" in Book of abstracts: 1st European Symposium on Phytochemicals in Medicine and Food, 1-EuSPMF; 2022 Sep 7-9; Belgrade, Serbia (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_5751 .