TY  - CONF
AU  - Zdravković-Korać, Snežana
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
AU  - Belić, Maja
AU  - Jevremović, Slađana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6713
AB  - Aesculus species are valuable ornamental plants, while their seeds are used as a source of health-promoting aescin. The somatic embryos (SE) of A. hippocastanum (horse chestnut) contain aescin in similar amounts as the seeds. Different pathways of propagation by tissue culture for several Aesculus species (A. hippocastanum, A. flava and A. carnea) have been investigated for few decades in our laboratory. Recently, an efficient protocol for somatic embryogenesis induction in stamen filaments culture has been developed. However, the frequency of embryogenic tissue (ET) induction varied markedly (0–50%) for the same genotypes from year to year. In A. flava, a significant increase (80–100%) in the frequency of ET induction was achieved by using liquid culture of friable calli. After the establishment of stable liquid cultures, lines with high ET proliferation and SE development were selected. Significant fresh weight increase (76–167-fold), with 256–669 newly developed SEs per 100 mg of initial inoculum, were achieved four weeks after plating on solid medium. In addition, most SEs (19–39%) reached the late torpedo and cotyledonary stage of development. SEs have high germination rate (80%), but conversion rates still need to be improved to enable sustained clonal propagation of elite specimens. The established protocols for ET induction and SE production represent a clean and safe technology for the in vitro production of Aesculus species, including alternative sources of aescin.
PB  - COST Action CA21157 COPYTREE
C3  - Book of Abstracts: The 2nd Conference of COST Action CA21157 COPYTREE: In vitro culture of woody crops: problem solving by new approaches; 2024 Apr 22-24; Jūrmala, Latvia
T1  - Recent advances in somatic embryogenesis induction in Aesculus species
SP  - 38
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6713
ER  - 

@conference{
author = "Zdravković-Korać, Snežana and Ćalić, Dušica and Milojević, Jelena and Belić, Maja and Jevremović, Slađana",
year = "2024",
abstract = "Aesculus species are valuable ornamental plants, while their seeds are used as a source of health-promoting aescin. The somatic embryos (SE) of A. hippocastanum (horse chestnut) contain aescin in similar amounts as the seeds. Different pathways of propagation by tissue culture for several Aesculus species (A. hippocastanum, A. flava and A. carnea) have been investigated for few decades in our laboratory. Recently, an efficient protocol for somatic embryogenesis induction in stamen filaments culture has been developed. However, the frequency of embryogenic tissue (ET) induction varied markedly (0–50%) for the same genotypes from year to year. In A. flava, a significant increase (80–100%) in the frequency of ET induction was achieved by using liquid culture of friable calli. After the establishment of stable liquid cultures, lines with high ET proliferation and SE development were selected. Significant fresh weight increase (76–167-fold), with 256–669 newly developed SEs per 100 mg of initial inoculum, were achieved four weeks after plating on solid medium. In addition, most SEs (19–39%) reached the late torpedo and cotyledonary stage of development. SEs have high germination rate (80%), but conversion rates still need to be improved to enable sustained clonal propagation of elite specimens. The established protocols for ET induction and SE production represent a clean and safe technology for the in vitro production of Aesculus species, including alternative sources of aescin.",
publisher = "COST Action CA21157 COPYTREE",
journal = "Book of Abstracts: The 2nd Conference of COST Action CA21157 COPYTREE: In vitro culture of woody crops: problem solving by new approaches; 2024 Apr 22-24; Jūrmala, Latvia",
title = "Recent advances in somatic embryogenesis induction in Aesculus species",
pages = "38",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6713"
}

Zdravković-Korać, S., Ćalić, D., Milojević, J., Belić, M.,& Jevremović, S.. (2024). Recent advances in somatic embryogenesis induction in Aesculus species. in Book of Abstracts: The 2nd Conference of COST Action CA21157 COPYTREE: In vitro culture of woody crops: problem solving by new approaches; 2024 Apr 22-24; Jūrmala, Latvia
COST Action CA21157 COPYTREE., 38.
https://hdl.handle.net/21.15107/rcub_ibiss_6713

Zdravković-Korać S, Ćalić D, Milojević J, Belić M, Jevremović S. Recent advances in somatic embryogenesis induction in Aesculus species. in Book of Abstracts: The 2nd Conference of COST Action CA21157 COPYTREE: In vitro culture of woody crops: problem solving by new approaches; 2024 Apr 22-24; Jūrmala, Latvia. 2024;:38.
https://hdl.handle.net/21.15107/rcub_ibiss_6713 .

Zdravković-Korać, Snežana, Ćalić, Dušica, Milojević, Jelena, Belić, Maja, Jevremović, Slađana, "Recent advances in somatic embryogenesis induction in Aesculus species" in Book of Abstracts: The 2nd Conference of COST Action CA21157 COPYTREE: In vitro culture of woody crops: problem solving by new approaches; 2024 Apr 22-24; Jūrmala, Latvia (2024):38,
https://hdl.handle.net/21.15107/rcub_ibiss_6713 .

TY  - JOUR
AU  - Ćalić, Dušica
AU  - Ristić-Đurović, Jasna L.
AU  - Ćirković, Saša
AU  - Milojević, Jelena
AU  - Belić, Maja
AU  - Stanišić, Mariana
AU  - Zdravković-Korać, Snežana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6420
AB  - Flax seeds stored in unfavorable conditions were exposed to static magnetic fields (SMFs)
of 50–350 mT for 20–120 min to overcome low germination and quality. Seed germination increased
slightly with increasing strength of SMF and duration of treatment. Seed germination from 89% to
100% was achieved in SMF treatments of 150 mT (120 min), 200 mT (80–120 mT), 250 mT (60–120 min),
300 mT (40–120 min), and 350 mT (40–120 min). In these treatments, germination was 2.78- to 3.12-fold
higher than in the control after 10 days. Treatments with 350 mT for 100 and 120 min showed the
best results in germination (100%), as well as a 26.81-fold increase in vigor I, 28.69-fold increase in
vigor II, 1-fold increase in chlorophyll a, 0.84-fold in chlorophyll b, 0.46-fold increase in carotenoid
content, and 2.63-fold increase in catalase activity compared to the control after 10 days. Also,
SMF treatment of 350 mT (20–120 min) reduced cell leakage and electrical conductivity by 1-fold
compared to the control. SMF is a healthy, biologically safe, and environmentally friendly treatment
and can be a tool for overcoming problems of low germination and quality of seeds stored under
unfavorable conditions
PB  - Basel: MDPI
T2  - Agriculture
T1  - Overcoming Low Germination and Low Quality of Flax Seeds (Linum usitatissimum L.) in Unfavorable Storage Using Static  Magnetic Fields
IS  - 11
VL  - 13
DO  - 10.3390/agriculture13112120
SP  - 2120
ER  - 

@article{
author = "Ćalić, Dušica and Ristić-Đurović, Jasna L. and Ćirković, Saša and Milojević, Jelena and Belić, Maja and Stanišić, Mariana and Zdravković-Korać, Snežana",
year = "2023",
abstract = "Flax seeds stored in unfavorable conditions were exposed to static magnetic fields (SMFs)
of 50–350 mT for 20–120 min to overcome low germination and quality. Seed germination increased
slightly with increasing strength of SMF and duration of treatment. Seed germination from 89% to
100% was achieved in SMF treatments of 150 mT (120 min), 200 mT (80–120 mT), 250 mT (60–120 min),
300 mT (40–120 min), and 350 mT (40–120 min). In these treatments, germination was 2.78- to 3.12-fold
higher than in the control after 10 days. Treatments with 350 mT for 100 and 120 min showed the
best results in germination (100%), as well as a 26.81-fold increase in vigor I, 28.69-fold increase in
vigor II, 1-fold increase in chlorophyll a, 0.84-fold in chlorophyll b, 0.46-fold increase in carotenoid
content, and 2.63-fold increase in catalase activity compared to the control after 10 days. Also,
SMF treatment of 350 mT (20–120 min) reduced cell leakage and electrical conductivity by 1-fold
compared to the control. SMF is a healthy, biologically safe, and environmentally friendly treatment
and can be a tool for overcoming problems of low germination and quality of seeds stored under
unfavorable conditions",
publisher = "Basel: MDPI",
journal = "Agriculture",
title = "Overcoming Low Germination and Low Quality of Flax Seeds (Linum usitatissimum L.) in Unfavorable Storage Using Static  Magnetic Fields",
number = "11",
volume = "13",
doi = "10.3390/agriculture13112120",
pages = "2120"
}

Ćalić, D., Ristić-Đurović, J. L., Ćirković, S., Milojević, J., Belić, M., Stanišić, M.,& Zdravković-Korać, S.. (2023). Overcoming Low Germination and Low Quality of Flax Seeds (Linum usitatissimum L.) in Unfavorable Storage Using Static  Magnetic Fields. in Agriculture
Basel: MDPI., 13(11), 2120.
https://doi.org/10.3390/agriculture13112120

Ćalić D, Ristić-Đurović JL, Ćirković S, Milojević J, Belić M, Stanišić M, Zdravković-Korać S. Overcoming Low Germination and Low Quality of Flax Seeds (Linum usitatissimum L.) in Unfavorable Storage Using Static  Magnetic Fields. in Agriculture. 2023;13(11):2120.
doi:10.3390/agriculture13112120 .

Ćalić, Dušica, Ristić-Đurović, Jasna L., Ćirković, Saša, Milojević, Jelena, Belić, Maja, Stanišić, Mariana, Zdravković-Korać, Snežana, "Overcoming Low Germination and Low Quality of Flax Seeds (Linum usitatissimum L.) in Unfavorable Storage Using Static  Magnetic Fields" in Agriculture, 13, no. 11 (2023):2120,
https://doi.org/10.3390/agriculture13112120 . .

TY  - JOUR
AU  - Zdravković-Korać, Snežana
AU  - Belić, Maja
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6090
AB  - A spinach-supplemented diet exerts numerous health benefits, but high levels of oxalic acid and nitrate can cause medical problems, so their levels should be reduced, while the levels of vitamins and phytochemicals could be further increased by breeding. Conventional spinach breeding is limited by the very complex sex determination. However, these limitations could be circumvented in synergy with a biotechnological approach. Accordingly, tissue culture techniques allow rapid and efficient clonal propagation of selected valuable genotypes, and somatic embryogenesis has been recognized as a superior process for clonal propagation because somatic embryos resemble zygotic embryos and therefore can spontaneously develop into complete plants. Since spinach has been considered recalcitrant to in vitro regeneration for decades, a deeper insight into the mechanisms underlying somatic embryogenesis is important for a better understanding and further improvement of the efficiency of this process. In this review, a comprehensive overview of the major factors affecting somatic embryogenesis in spinach is presented and discussed, with particular
emphasis on the synergistic effects of α–naphthaleneacetic acid, gibberellic acid, light, and the intrinsic predisposition of individual seedlings to somatic embryogenesis, as well as the expression of genes encoding key enzymes involved in the maintenance of gibberellin homeostasis and the levels of endogenous gibberellins.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Somatic Embryogenesis in Spinach—A Review
IS  - 9
VL  - 9
DO  - 10.3390/horticulturae9091048
SP  - 1048
ER  - 

@article{
author = "Zdravković-Korać, Snežana and Belić, Maja and Ćalić, Dušica and Milojević, Jelena",
year = "2023",
abstract = "A spinach-supplemented diet exerts numerous health benefits, but high levels of oxalic acid and nitrate can cause medical problems, so their levels should be reduced, while the levels of vitamins and phytochemicals could be further increased by breeding. Conventional spinach breeding is limited by the very complex sex determination. However, these limitations could be circumvented in synergy with a biotechnological approach. Accordingly, tissue culture techniques allow rapid and efficient clonal propagation of selected valuable genotypes, and somatic embryogenesis has been recognized as a superior process for clonal propagation because somatic embryos resemble zygotic embryos and therefore can spontaneously develop into complete plants. Since spinach has been considered recalcitrant to in vitro regeneration for decades, a deeper insight into the mechanisms underlying somatic embryogenesis is important for a better understanding and further improvement of the efficiency of this process. In this review, a comprehensive overview of the major factors affecting somatic embryogenesis in spinach is presented and discussed, with particular
emphasis on the synergistic effects of α–naphthaleneacetic acid, gibberellic acid, light, and the intrinsic predisposition of individual seedlings to somatic embryogenesis, as well as the expression of genes encoding key enzymes involved in the maintenance of gibberellin homeostasis and the levels of endogenous gibberellins.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Somatic Embryogenesis in Spinach—A Review",
number = "9",
volume = "9",
doi = "10.3390/horticulturae9091048",
pages = "1048"
}

Zdravković-Korać, S., Belić, M., Ćalić, D.,& Milojević, J.. (2023). Somatic Embryogenesis in Spinach—A Review. in Horticulturae
Basel: MDPI., 9(9), 1048.
https://doi.org/10.3390/horticulturae9091048

Zdravković-Korać S, Belić M, Ćalić D, Milojević J. Somatic Embryogenesis in Spinach—A Review. in Horticulturae. 2023;9(9):1048.
doi:10.3390/horticulturae9091048 .

Zdravković-Korać, Snežana, Belić, Maja, Ćalić, Dušica, Milojević, Jelena, "Somatic Embryogenesis in Spinach—A Review" in Horticulturae, 9, no. 9 (2023):1048,
https://doi.org/10.3390/horticulturae9091048 . .

TY  - JOUR
AU  - Kostić, Igor
AU  - Milanović, Slobodan
AU  - Kostić, Miroslav
AU  - Šešlija Jovanović, Darka
AU  - Ćalić, Dušica
AU  - Jankovský, Libor
AU  - Lazarević, Jelica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5299
AB  - Botanicals, such as essential oils (EO) and their compounds, are considered a viable ecofriendly
alternative to synthetic insecticides, which threaten human health and ecosystem functioning.
In the present study, we explored the potential use of two EO compounds, trans-anethole (phenylpropanoid)
and S-(+)-carvone (monoterpene ketone), against gypsy moth larvae (GML), a serious
pest of deciduous forests and orchards. GML feeding, survival, molting, and nutritional physiology
were assessed at different compound concentrations and compared with the effects of the commercial
botanical product NeemAzal®-T/S (neem). The impact of botanicals on GML feeding was assessed
by the leaf-dipping method and showed the highest antifeeding activity of neem in the no-choice
assay. GML that were offered a choice were deterred by anethole and attracted by low concentrations
of carvone and neem. Ingestion of botanicals was more effective in inducing mortality and reducing
molting than residual contact exposure. Anethole and carvone were better toxicants but worse growth
regulators than neem. Assessing nutritional indices revealed reduced growth, consumption, and
food utilization in larvae fed on botanical-supplemented diets. The highest metabolic cost of food
processing was recorded in carvone-fed larvae, which exhibited a negative growth rate. The results
suggest that anethole and carvone might be used as control agents against GML.
PB  - Basel: MDPI
T2  - Agronomy
T1  - Antifeeding, Toxic, and Growth-Reducing Activity of trans-Anethole and S-(+)-Carvone against Larvae of the Gypsy Moth Lymantria dispar (L.)
IS  - 12
VL  - 12
DO  - 10.3390/agronomy12123049
SP  - 3049
ER  - 

@article{
author = "Kostić, Igor and Milanović, Slobodan and Kostić, Miroslav and Šešlija Jovanović, Darka and Ćalić, Dušica and Jankovský, Libor and Lazarević, Jelica",
year = "2022",
abstract = "Botanicals, such as essential oils (EO) and their compounds, are considered a viable ecofriendly
alternative to synthetic insecticides, which threaten human health and ecosystem functioning.
In the present study, we explored the potential use of two EO compounds, trans-anethole (phenylpropanoid)
and S-(+)-carvone (monoterpene ketone), against gypsy moth larvae (GML), a serious
pest of deciduous forests and orchards. GML feeding, survival, molting, and nutritional physiology
were assessed at different compound concentrations and compared with the effects of the commercial
botanical product NeemAzal®-T/S (neem). The impact of botanicals on GML feeding was assessed
by the leaf-dipping method and showed the highest antifeeding activity of neem in the no-choice
assay. GML that were offered a choice were deterred by anethole and attracted by low concentrations
of carvone and neem. Ingestion of botanicals was more effective in inducing mortality and reducing
molting than residual contact exposure. Anethole and carvone were better toxicants but worse growth
regulators than neem. Assessing nutritional indices revealed reduced growth, consumption, and
food utilization in larvae fed on botanical-supplemented diets. The highest metabolic cost of food
processing was recorded in carvone-fed larvae, which exhibited a negative growth rate. The results
suggest that anethole and carvone might be used as control agents against GML.",
publisher = "Basel: MDPI",
journal = "Agronomy",
title = "Antifeeding, Toxic, and Growth-Reducing Activity of trans-Anethole and S-(+)-Carvone against Larvae of the Gypsy Moth Lymantria dispar (L.)",
number = "12",
volume = "12",
doi = "10.3390/agronomy12123049",
pages = "3049"
}

Kostić, I., Milanović, S., Kostić, M., Šešlija Jovanović, D., Ćalić, D., Jankovský, L.,& Lazarević, J.. (2022). Antifeeding, Toxic, and Growth-Reducing Activity of trans-Anethole and S-(+)-Carvone against Larvae of the Gypsy Moth Lymantria dispar (L.). in Agronomy
Basel: MDPI., 12(12), 3049.
https://doi.org/10.3390/agronomy12123049

Kostić I, Milanović S, Kostić M, Šešlija Jovanović D, Ćalić D, Jankovský L, Lazarević J. Antifeeding, Toxic, and Growth-Reducing Activity of trans-Anethole and S-(+)-Carvone against Larvae of the Gypsy Moth Lymantria dispar (L.). in Agronomy. 2022;12(12):3049.
doi:10.3390/agronomy12123049 .

Kostić, Igor, Milanović, Slobodan, Kostić, Miroslav, Šešlija Jovanović, Darka, Ćalić, Dušica, Jankovský, Libor, Lazarević, Jelica, "Antifeeding, Toxic, and Growth-Reducing Activity of trans-Anethole and S-(+)-Carvone against Larvae of the Gypsy Moth Lymantria dispar (L.)" in Agronomy, 12, no. 12 (2022):3049,
https://doi.org/10.3390/agronomy12123049 . .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Kostić, Igor
AU  - Šešlija Jovanović, Darka
AU  - Ćalić, Dušica
AU  - Milanović, Slobodan
AU  - Kostić, Miroslav
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5306
AB  - The Colorado potato beetle (CPB) is a serious pest of economically important Solanaceae
species. The use of essential oil compounds in pest management has been proposed as an alternative
to harmful chemical insecticides that disturb human health and ecosystem functioning. We examined
the antifeedant activity of three concentrations (0.125%, 0.25% and 0.5%) of pure camphor and
a thujone‐camphor mixture against 3rd instar larvae and adults. Their efficacy was evaluated according
to the degree of leaf damage and avoidance of treated leaves by the CPB. Treatment of
potato leaves significantly reduced leaf damage compared to the control. Leaf protection increased
at higher concentrations of the examined compounds. Camphor was more effective against larvae
and the thujone‐camphor mixture was more effective against adults. Additionally, adults moved
faster towards the control leaf disc in the two‐choice olfactometer assay if an alternative disc was
treated with a thujone‐camphor mixture, whereas larvae responded similarly to the two potential
repellents. However, after contact with the leaf disc treated with the highest compound concentration,
the larvae escaped faster from the thujone‐camphor mixture than from pure camphor. In conclusion,
both examined compounds are promising eco‐friendly antifeedants, but their efficacy depends
on the developmental stage of the beetle, compound type and applied concentration.
PB  - Basel: MDPI
T2  - Plants
T1  - Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle
IS  - 24
VL  - 11
DO  - 10.3390/plants11243587
SP  - 3587
ER  - 

@article{
author = "Lazarević, Jelica and Kostić, Igor and Šešlija Jovanović, Darka and Ćalić, Dušica and Milanović, Slobodan and Kostić, Miroslav",
year = "2022",
abstract = "The Colorado potato beetle (CPB) is a serious pest of economically important Solanaceae
species. The use of essential oil compounds in pest management has been proposed as an alternative
to harmful chemical insecticides that disturb human health and ecosystem functioning. We examined
the antifeedant activity of three concentrations (0.125%, 0.25% and 0.5%) of pure camphor and
a thujone‐camphor mixture against 3rd instar larvae and adults. Their efficacy was evaluated according
to the degree of leaf damage and avoidance of treated leaves by the CPB. Treatment of
potato leaves significantly reduced leaf damage compared to the control. Leaf protection increased
at higher concentrations of the examined compounds. Camphor was more effective against larvae
and the thujone‐camphor mixture was more effective against adults. Additionally, adults moved
faster towards the control leaf disc in the two‐choice olfactometer assay if an alternative disc was
treated with a thujone‐camphor mixture, whereas larvae responded similarly to the two potential
repellents. However, after contact with the leaf disc treated with the highest compound concentration,
the larvae escaped faster from the thujone‐camphor mixture than from pure camphor. In conclusion,
both examined compounds are promising eco‐friendly antifeedants, but their efficacy depends
on the developmental stage of the beetle, compound type and applied concentration.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle",
number = "24",
volume = "11",
doi = "10.3390/plants11243587",
pages = "3587"
}

Lazarević, J., Kostić, I., Šešlija Jovanović, D., Ćalić, D., Milanović, S.,& Kostić, M.. (2022). Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle. in Plants
Basel: MDPI., 11(24), 3587.
https://doi.org/10.3390/plants11243587

Lazarević J, Kostić I, Šešlija Jovanović D, Ćalić D, Milanović S, Kostić M. Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle. in Plants. 2022;11(24):3587.
doi:10.3390/plants11243587 .

Lazarević, Jelica, Kostić, Igor, Šešlija Jovanović, Darka, Ćalić, Dušica, Milanović, Slobodan, Kostić, Miroslav, "Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle" in Plants, 11, no. 24 (2022):3587,
https://doi.org/10.3390/plants11243587 . .

TY  - JOUR
AU  - Smailagić, Dijana
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Savić, Jelena
AU  - Ćosić, Tatjana
AU  - Pěnčík, Aleš
AU  - Ćalić, Dušica
AU  - Bogdanović, Milica
AU  - Trajković, Milena
AU  - Stanišić, Mariana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5065
AB  - Apple species are the unique naturally rich source of dihydrochalcones, phenolic compounds with an elusive role in planta, but suggested auto-allelochemical features related to “apple replant disease” (ARD). Our aim was to elucidate the physiological basis of the phytotoxic action of dihydrochalcone phloretin in the model plant Arabidopsis and to promote phloretin as a new prospective eco-friendly phytotoxic compound. Phloretin treatment induced a significant dose-dependent growth retardation and severe morphological abnormalities and agravitropic behavior in Arabidopsis seedlings. Histological examination revealed a reduced starch content in the columella cells and a serious disturbance in root architecture, which resulted in the reduction in length of meristematic and elongation zones. Significantly disturbed auxin metabolome profile in roots with a particularly increased content of IAA accumulated in the lateral parts of the root apex, accompanied by changes in the expression of auxin biosynthetic and transport genes, especially PIN1, PIN3, PIN7, and ABCB1, indicates the role of auxin in physiological basis of phloretin-induced growth retardation. The results reveal a disturbance of auxin homeostasis as the main mechanism of phytotoxic action of phloretin. This mechanism makes phloretin a prospective candidate for an eco-friendly bioherbicide and paves the way for further research of phloretin role in ARD.
PB  - Lausanne: Frontiers Media SA
T2  - Frontiers in Plant Science
T1  - New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action
VL  - 13
DO  - 0.3389/fpls.2022.875528
SP  - 875528
ER  - 

@article{
author = "Smailagić, Dijana and Banjac, Nevena and Ninković, Slavica and Savić, Jelena and Ćosić, Tatjana and Pěnčík, Aleš and Ćalić, Dušica and Bogdanović, Milica and Trajković, Milena and Stanišić, Mariana",
year = "2022",
abstract = "Apple species are the unique naturally rich source of dihydrochalcones, phenolic compounds with an elusive role in planta, but suggested auto-allelochemical features related to “apple replant disease” (ARD). Our aim was to elucidate the physiological basis of the phytotoxic action of dihydrochalcone phloretin in the model plant Arabidopsis and to promote phloretin as a new prospective eco-friendly phytotoxic compound. Phloretin treatment induced a significant dose-dependent growth retardation and severe morphological abnormalities and agravitropic behavior in Arabidopsis seedlings. Histological examination revealed a reduced starch content in the columella cells and a serious disturbance in root architecture, which resulted in the reduction in length of meristematic and elongation zones. Significantly disturbed auxin metabolome profile in roots with a particularly increased content of IAA accumulated in the lateral parts of the root apex, accompanied by changes in the expression of auxin biosynthetic and transport genes, especially PIN1, PIN3, PIN7, and ABCB1, indicates the role of auxin in physiological basis of phloretin-induced growth retardation. The results reveal a disturbance of auxin homeostasis as the main mechanism of phytotoxic action of phloretin. This mechanism makes phloretin a prospective candidate for an eco-friendly bioherbicide and paves the way for further research of phloretin role in ARD.",
publisher = "Lausanne: Frontiers Media SA",
journal = "Frontiers in Plant Science",
title = "New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action",
volume = "13",
doi = "0.3389/fpls.2022.875528",
pages = "875528"
}

Smailagić, D., Banjac, N., Ninković, S., Savić, J., Ćosić, T., Pěnčík, A., Ćalić, D., Bogdanović, M., Trajković, M.,& Stanišić, M.. (2022). New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action. in Frontiers in Plant Science
Lausanne: Frontiers Media SA., 13, 875528.
https://doi.org/0.3389/fpls.2022.875528

Smailagić D, Banjac N, Ninković S, Savić J, Ćosić T, Pěnčík A, Ćalić D, Bogdanović M, Trajković M, Stanišić M. New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action. in Frontiers in Plant Science. 2022;13:875528.
doi:0.3389/fpls.2022.875528 .

Smailagić, Dijana, Banjac, Nevena, Ninković, Slavica, Savić, Jelena, Ćosić, Tatjana, Pěnčík, Aleš, Ćalić, Dušica, Bogdanović, Milica, Trajković, Milena, Stanišić, Mariana, "New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action" in Frontiers in Plant Science, 13 (2022):875528,
https://doi.org/0.3389/fpls.2022.875528 . .

TY  - JOUR
AU  - Zdravković-Korać, Snežana
AU  - Milojević, Jelena
AU  - Belić, Maja
AU  - Ćalić, Dušica
PY  - 2022
UR  - https://www.mdpi.com/2223-7747/11/3/277
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4777
AB  - Species of the genus Aesculus are very attractive woody ornamentals. Their organs contain numerous health-promoting phytochemicals. The most valuable of them—aescin—is used in commercial preparations for the treatment of venous insufficiency. The industrial source of aescin is horse chestnut seeds because the zygotic embryos are the main site of its accumulation. Horse chestnut somatic and zygotic embryos contain similar amount of aescin, hence somatic embryos could be exploited as an alternative source of aescin. Somatic embryogenesis, androgenesis and de novo shoot organogenesis were successfully achieved in several Aesculus species, as well as secondary somatic embryogenesis and shoot organogenesis, which enables mass production of embryos and shoots. In addition, an efficient method for cryopreservation of embryogenic tissue was established, assuring constant availability of the plant material. The developed methods are suitable for clonal propagation of elite specimens selected as the best aescin producers, the most attractive ornamentals or plants resistant to pests and diseases. These methods are also useful for molecular breeding purposes. Thus, in this review, the medicinal uses and a comprehensive survey of in vitro propagation methods established for Aesculus species, as well as the feasibility of in vitro production of aescin, are presented and discussed.
PB  - Basel: MDPI
T2  - Plants
T1  - Tissue Culture Response of Ornamental and Medicinal Aesculus Species—A Review
IS  - 3
VL  - 11
DO  - 10.3390/plants11030277
SP  - 277
ER  - 

@article{
author = "Zdravković-Korać, Snežana and Milojević, Jelena and Belić, Maja and Ćalić, Dušica",
year = "2022",
abstract = "Species of the genus Aesculus are very attractive woody ornamentals. Their organs contain numerous health-promoting phytochemicals. The most valuable of them—aescin—is used in commercial preparations for the treatment of venous insufficiency. The industrial source of aescin is horse chestnut seeds because the zygotic embryos are the main site of its accumulation. Horse chestnut somatic and zygotic embryos contain similar amount of aescin, hence somatic embryos could be exploited as an alternative source of aescin. Somatic embryogenesis, androgenesis and de novo shoot organogenesis were successfully achieved in several Aesculus species, as well as secondary somatic embryogenesis and shoot organogenesis, which enables mass production of embryos and shoots. In addition, an efficient method for cryopreservation of embryogenic tissue was established, assuring constant availability of the plant material. The developed methods are suitable for clonal propagation of elite specimens selected as the best aescin producers, the most attractive ornamentals or plants resistant to pests and diseases. These methods are also useful for molecular breeding purposes. Thus, in this review, the medicinal uses and a comprehensive survey of in vitro propagation methods established for Aesculus species, as well as the feasibility of in vitro production of aescin, are presented and discussed.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Tissue Culture Response of Ornamental and Medicinal Aesculus Species—A Review",
number = "3",
volume = "11",
doi = "10.3390/plants11030277",
pages = "277"
}

Zdravković-Korać, S., Milojević, J., Belić, M.,& Ćalić, D.. (2022). Tissue Culture Response of Ornamental and Medicinal Aesculus Species—A Review. in Plants
Basel: MDPI., 11(3), 277.
https://doi.org/10.3390/plants11030277

Zdravković-Korać S, Milojević J, Belić M, Ćalić D. Tissue Culture Response of Ornamental and Medicinal Aesculus Species—A Review. in Plants. 2022;11(3):277.
doi:10.3390/plants11030277 .

Zdravković-Korać, Snežana, Milojević, Jelena, Belić, Maja, Ćalić, Dušica, "Tissue Culture Response of Ornamental and Medicinal Aesculus Species—A Review" in Plants, 11, no. 3 (2022):277,
https://doi.org/10.3390/plants11030277 . .

TY  - CONF
AU  - Zdravković-Korać, Snežana
AU  - Belić, Maja
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5331
AB  - Epigenetic modifications of chromatin play a pivotal role in regulation of expression of genes involved in somatic embryo (SE) induction. Hence, the compounds which affect DNA-histone interaction may trigger somatic embryogenesis. Trichostatin (TSA) is a potent inhibitor of histone deacetylases, whose activity leads to increased histone acetylation, thereby affecting gene expression. To explore epigenetic control of SE regeneration from root apices (1 cm) of spinach seedlings, the explants were cultivated on media supplemented with 0, 0.1, 0.5, 1 or 5 μM TSA + 0, 1, 10 or 20 μM α naphthaleneacetic acid (NAA) + 0 or 5 μM gibberellic acid (GA3). The explants were exposed to TSA for 1, 7 or 14 days, and subsequently subcultivated on TSA free medium of the same composition. TSA was dissolved in dimethyl sulfoxide (DMSO), whose final concentration in all media, including TSA-free controls, was 0.05%. TSA was not sufficient to induce SEs either alone or in combination with NAA or GA3. SE regeneration was observed only from the explants cultivated on media supplemented with 10 or 20 μM NAA + 5 μM GA3. In both combinations, TSA promoted somatic embryogenesis, but longer TSA treatment was needed with 10 μM NAA than with 20 μMNAA for efficient SE induction. The highest embryogenic response was attained with 0.1-0.5 μM TSA. DMSO also significantly improved SE induction, probably by enhancing NAA and GA3 intake into the plant cells. The results indicate a significant role of epigenetic control in SE induction in spinach.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Trichostatin and dimethyl sulfoxide enhance somatic embryogenesis from root apices of spinach
SP  - 25
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5331
ER  - 

@conference{
author = "Zdravković-Korać, Snežana and Belić, Maja and Ćalić, Dušica and Milojević, Jelena",
year = "2022",
abstract = "Epigenetic modifications of chromatin play a pivotal role in regulation of expression of genes involved in somatic embryo (SE) induction. Hence, the compounds which affect DNA-histone interaction may trigger somatic embryogenesis. Trichostatin (TSA) is a potent inhibitor of histone deacetylases, whose activity leads to increased histone acetylation, thereby affecting gene expression. To explore epigenetic control of SE regeneration from root apices (1 cm) of spinach seedlings, the explants were cultivated on media supplemented with 0, 0.1, 0.5, 1 or 5 μM TSA + 0, 1, 10 or 20 μM α naphthaleneacetic acid (NAA) + 0 or 5 μM gibberellic acid (GA3). The explants were exposed to TSA for 1, 7 or 14 days, and subsequently subcultivated on TSA free medium of the same composition. TSA was dissolved in dimethyl sulfoxide (DMSO), whose final concentration in all media, including TSA-free controls, was 0.05%. TSA was not sufficient to induce SEs either alone or in combination with NAA or GA3. SE regeneration was observed only from the explants cultivated on media supplemented with 10 or 20 μM NAA + 5 μM GA3. In both combinations, TSA promoted somatic embryogenesis, but longer TSA treatment was needed with 10 μM NAA than with 20 μMNAA for efficient SE induction. The highest embryogenic response was attained with 0.1-0.5 μM TSA. DMSO also significantly improved SE induction, probably by enhancing NAA and GA3 intake into the plant cells. The results indicate a significant role of epigenetic control in SE induction in spinach.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Trichostatin and dimethyl sulfoxide enhance somatic embryogenesis from root apices of spinach",
pages = "25",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5331"
}

Zdravković-Korać, S., Belić, M., Ćalić, D.,& Milojević, J.. (2022). Trichostatin and dimethyl sulfoxide enhance somatic embryogenesis from root apices of spinach. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 25.
https://hdl.handle.net/21.15107/rcub_ibiss_5331

Zdravković-Korać S, Belić M, Ćalić D, Milojević J. Trichostatin and dimethyl sulfoxide enhance somatic embryogenesis from root apices of spinach. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:25.
https://hdl.handle.net/21.15107/rcub_ibiss_5331 .

Zdravković-Korać, Snežana, Belić, Maja, Ćalić, Dušica, Milojević, Jelena, "Trichostatin and dimethyl sulfoxide enhance somatic embryogenesis from root apices of spinach" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):25,
https://hdl.handle.net/21.15107/rcub_ibiss_5331 .

TY  - CONF
AU  - Ćalić, Dušica
AU  - Ristić Djurović, Jasna
AU  - Ćirković, Saša
AU  - Milojević, Jelena
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5152
AB  - Стимулација семена магнетним пољем је биолошки безбедна, јефтина, популарна, еколошки прихватљива и неинвазивна метода са благотворним ефектима на биљке. Примена магнетних поља смањује употребу пестицида, инсектицида и омогућава производњу еколошки безбедне хране (Maffei1), што је у скаду са стратегијом ЕУ “Од њиве до вилице” (“Farm to fork”). Стратегија предвиђа да се до 2030. године смањи употреба пестицида за 50% и обезбеди удео еколошке пољопривредне производње од 25% у укупној пољопривреди. С обзиром на тренутних 0.60% површина под органском производњом у Србији, значајније повећање постаје реално само уз примену иновативних начина гајења пољопривредних култура. У овој студији, магнетна поља различитих јачина (50, 100 и 300 мТ) и времена експозиције показала су позитиван ефекат на клијавост семена купуса, раст клијанаца, дужину и ширину корена, број формираних листова, масу главице, садржај хлорофила, каротеноида и ксантофила, садржај каталаза и пероксидаза, витамина Ц, микро и макро елемената. У листовима третираног купуса детектовано је до 50 % више гвожђа, и два пута мање натријума него у контроли. Принос третираног купуса на огледном пољу земљорадничке задруге „Пољо-флора“ нашег највећег произвођача и извозника киселог купуса је 30 % повећан у односу на контролу. Добијени резултати сугеришу да се магнетна поља могу користити у органској пољопривреди и производњи здраве хране, што је у складу са стратегијом ЕУ.
AB  - Stimulacija semena magnetnim poljem je biološki bezbedna, jeftina, popularna, ekološki prihvatljiva i neinvazivna metoda sa blagotvornim efektima na biljke. Primena magnetnih polja smanjuje upotrebu pesticida, insekticida i omogućava proizvodnju ekološki bezbedne hrane (Maffei1), što je u skadu sa strategijom EU “Od njive do vilice” (“Farm to fork”). Strategija predviđa da se do 2030. godine smanji upotreba pesticida za 50% i obezbedi udeo ekološke poljoprivredne proizvodnje od 25% u ukupnoj poljoprivredi. S obzirom na trenutnih 0.60% površina pod organskom proizvodnjom u Srbiji, značajnije povećanje postaje realno samo uz primenu inovativnih načina gajenja poljoprivrednih kultura. U ovoj studiji, magnetna polja različitih jačina (50, 100 i 300 mT) i vremena ekspozicije pokazala su pozitivan efekat na klijavost semena kupusa, rast klijanaca, dužinu i širinu korena, broj formiranih listova, masu glavice, sadržaj hlorofila, karotenoida i ksantofila, sadržaj katalaza i peroksidaza, vitamina C, mikro i makro elemenata. U listovima tretiranog kupusa detektovano je do 50 % više gvožđa, i dva puta manje natrijuma nego u kontroli. Prinos tretiranog kupusa na oglednom polju zemljoradničke zadruge „Poljo-flora“ našeg najvećeg proizvođača i izvoznika kiselog kupusa je 30 % povećan u odnosu na kontrolu. Dobijeni rezultati sugerišu da se magnetna polja mogu koristiti u organskoj poljoprivredi i proizvodnji zdrave hrane, što je u skladu sa strategijom EU.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Могу ли магнети помоћи биљкама?
T1  - Mogu li magneti pomoći biljkama?
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5152
ER  - 

@conference{
author = "Ćalić, Dušica and Ristić Djurović, Jasna and Ćirković, Saša and Milojević, Jelena and Belić, Maja and Zdravković-Korać, Snežana",
year = "2022",
abstract = "Стимулација семена магнетним пољем је биолошки безбедна, јефтина, популарна, еколошки прихватљива и неинвазивна метода са благотворним ефектима на биљке. Примена магнетних поља смањује употребу пестицида, инсектицида и омогућава производњу еколошки безбедне хране (Maffei1), што је у скаду са стратегијом ЕУ “Од њиве до вилице” (“Farm to fork”). Стратегија предвиђа да се до 2030. године смањи употреба пестицида за 50% и обезбеди удео еколошке пољопривредне производње од 25% у укупној пољопривреди. С обзиром на тренутних 0.60% површина под органском производњом у Србији, значајније повећање постаје реално само уз примену иновативних начина гајења пољопривредних култура. У овој студији, магнетна поља различитих јачина (50, 100 и 300 мТ) и времена експозиције показала су позитиван ефекат на клијавост семена купуса, раст клијанаца, дужину и ширину корена, број формираних листова, масу главице, садржај хлорофила, каротеноида и ксантофила, садржај каталаза и пероксидаза, витамина Ц, микро и макро елемената. У листовима третираног купуса детектовано је до 50 % више гвожђа, и два пута мање натријума него у контроли. Принос третираног купуса на огледном пољу земљорадничке задруге „Пољо-флора“ нашег највећег произвођача и извозника киселог купуса је 30 % повећан у односу на контролу. Добијени резултати сугеришу да се магнетна поља могу користити у органској пољопривреди и производњи здраве хране, што је у складу са стратегијом ЕУ., Stimulacija semena magnetnim poljem je biološki bezbedna, jeftina, popularna, ekološki prihvatljiva i neinvazivna metoda sa blagotvornim efektima na biljke. Primena magnetnih polja smanjuje upotrebu pesticida, insekticida i omogućava proizvodnju ekološki bezbedne hrane (Maffei1), što je u skadu sa strategijom EU “Od njive do vilice” (“Farm to fork”). Strategija predviđa da se do 2030. godine smanji upotreba pesticida za 50% i obezbedi udeo ekološke poljoprivredne proizvodnje od 25% u ukupnoj poljoprivredi. S obzirom na trenutnih 0.60% površina pod organskom proizvodnjom u Srbiji, značajnije povećanje postaje realno samo uz primenu inovativnih načina gajenja poljoprivrednih kultura. U ovoj studiji, magnetna polja različitih jačina (50, 100 i 300 mT) i vremena ekspozicije pokazala su pozitivan efekat na klijavost semena kupusa, rast klijanaca, dužinu i širinu korena, broj formiranih listova, masu glavice, sadržaj hlorofila, karotenoida i ksantofila, sadržaj katalaza i peroksidaza, vitamina C, mikro i makro elemenata. U listovima tretiranog kupusa detektovano je do 50 % više gvožđa, i dva puta manje natrijuma nego u kontroli. Prinos tretiranog kupusa na oglednom polju zemljoradničke zadruge „Poljo-flora“ našeg najvećeg proizvođača i izvoznika kiselog kupusa je 30 % povećan u odnosu na kontrolu. Dobijeni rezultati sugerišu da se magnetna polja mogu koristiti u organskoj poljoprivredi i proizvodnji zdrave hrane, što je u skladu sa strategijom EU.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Могу ли магнети помоћи биљкама?, Mogu li magneti pomoći biljkama?",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5152"
}

Ćalić, D., Ristić Djurović, J., Ćirković, S., Milojević, J., Belić, M.,& Zdravković-Korać, S.. (2022). Могу ли магнети помоћи биљкама?. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society..
https://hdl.handle.net/21.15107/rcub_ibiss_5152

Ćalić D, Ristić Djurović J, Ćirković S, Milojević J, Belić M, Zdravković-Korać S. Могу ли магнети помоћи биљкама?. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_5152 .

Ćalić, Dušica, Ristić Djurović, Jasna, Ćirković, Saša, Milojević, Jelena, Belić, Maja, Zdravković-Korać, Snežana, "Могу ли магнети помоћи биљкама?" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_5152 .

TY  - CONF
AU  - Jevremović, Slađana
AU  - Ćalić, Dušica
AU  - Zdravković-Korać, Snežana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5153
AB  - Идеја о вештачким семенима датира још од 1977. године када је Murashige1 предложио да вештачка семена представљају „инкапсулиране соматске ембрионе“. Данас се под термином вештачка (синтетичка, артифицијална) семена предразумева било који инкапсулирани биљни материјал (соматски ембриони, врхови апикалних или аксиларних изданака, ћелијски агрегати) који се може користити за гајење, чување, пропагацију и добијање комплетних биљака. Основа технологије вештачких семена је имитирање услова за нормално растење и развиће биљака као што се дешава током клијања и формирања биљака из семена. Технологија вештачких семена је искоришћена и као део појединих протокола за краткотрајно чување биљног материјала методама успореног растења као и за његово дуготрајно чување методама криопрезервације. Ова технологија је од великог значаја за биљне врсте које производе невијабилна семена, семена која тешко клијају, формирају мали број семена или их уопште не производе. Неке од таквих биљних врста су и заштићене и угрожене врсте које најчешће показују проблеме у развићу семена и репродукцији. Такође, ова технологија се показала као погодна за пропагацију и чување различитих дрвенастих, лековитих или украсних биљака које се вегетативно умножавају. У раду ће бити приказана методологија добијања вештачких семена као и досадашњи резултати на примени технологије пропагације врхова изданака хризантема и ћелијских суспензија кестена.
AB  - Ideja o veštačkim semenima datira još od 1977. godine kada je Murashige1 predložio da veštačka semena predstavljaju „inkapsulirane somatske embrione“. Danas se pod terminom veštačka (sintetička, artificijalna) semena predrazumeva bilo koji inkapsulirani biljni materijal (somatski embrioni, vrhovi apikalnih ili aksilarnih izdanaka, ćelijski agregati) koji se može koristiti za gajenje, čuvanje, propagaciju i dobijanje kompletnih biljaka. Osnova tehnologije veštačkih semena je imitiranje uslova za normalno rastenje i razviće biljaka kao što se dešava tokom klijanja i formiranja biljaka iz semena. Tehnologija veštačkih semena je iskorišćena i kao deo pojedinih protokola za kratkotrajno čuvanje biljnog materijala metodama usporenog rastenja kao i za njegovo dugotrajno čuvanje metodama krioprezervacije. Ova tehnologija je od velikog značaja za biljne vrste koje proizvode nevijabilna semena, semena koja teško klijaju, formiraju mali broj semena ili ih uopšte ne proizvode. Neke od takvih biljnih vrsta su i zaštićene i ugrožene vrste koje najčešće pokazuju probleme u razviću semena i reprodukciji. Takođe, ova tehnologija se pokazala kao pogodna za propagaciju i čuvanje različitih drvenastih, lekovitih ili ukrasnih biljaka koje se vegetativno umnožavaju. U radu će biti prikazana metodologija dobijanja veštačkih semena kao i dosadašnji rezultati na primeni tehnologije propagacije vrhova izdanaka hrizantema i ćelijskih suspenzija kestena.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Примена технологије вештачких семена у пропагацији и  конзервацији биљака
T1  - Primena tehnologije veštačkih semena u propagaciji i konzervaciji biljaka
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5153
ER  - 

@conference{
author = "Jevremović, Slađana and Ćalić, Dušica and Zdravković-Korać, Snežana",
year = "2022",
abstract = "Идеја о вештачким семенима датира још од 1977. године када је Murashige1 предложио да вештачка семена представљају „инкапсулиране соматске ембрионе“. Данас се под термином вештачка (синтетичка, артифицијална) семена предразумева било који инкапсулирани биљни материјал (соматски ембриони, врхови апикалних или аксиларних изданака, ћелијски агрегати) који се може користити за гајење, чување, пропагацију и добијање комплетних биљака. Основа технологије вештачких семена је имитирање услова за нормално растење и развиће биљака као што се дешава током клијања и формирања биљака из семена. Технологија вештачких семена је искоришћена и као део појединих протокола за краткотрајно чување биљног материјала методама успореног растења као и за његово дуготрајно чување методама криопрезервације. Ова технологија је од великог значаја за биљне врсте које производе невијабилна семена, семена која тешко клијају, формирају мали број семена или их уопште не производе. Неке од таквих биљних врста су и заштићене и угрожене врсте које најчешће показују проблеме у развићу семена и репродукцији. Такође, ова технологија се показала као погодна за пропагацију и чување различитих дрвенастих, лековитих или украсних биљака које се вегетативно умножавају. У раду ће бити приказана методологија добијања вештачких семена као и досадашњи резултати на примени технологије пропагације врхова изданака хризантема и ћелијских суспензија кестена., Ideja o veštačkim semenima datira još od 1977. godine kada je Murashige1 predložio da veštačka semena predstavljaju „inkapsulirane somatske embrione“. Danas se pod terminom veštačka (sintetička, artificijalna) semena predrazumeva bilo koji inkapsulirani biljni materijal (somatski embrioni, vrhovi apikalnih ili aksilarnih izdanaka, ćelijski agregati) koji se može koristiti za gajenje, čuvanje, propagaciju i dobijanje kompletnih biljaka. Osnova tehnologije veštačkih semena je imitiranje uslova za normalno rastenje i razviće biljaka kao što se dešava tokom klijanja i formiranja biljaka iz semena. Tehnologija veštačkih semena je iskorišćena i kao deo pojedinih protokola za kratkotrajno čuvanje biljnog materijala metodama usporenog rastenja kao i za njegovo dugotrajno čuvanje metodama krioprezervacije. Ova tehnologija je od velikog značaja za biljne vrste koje proizvode nevijabilna semena, semena koja teško klijaju, formiraju mali broj semena ili ih uopšte ne proizvode. Neke od takvih biljnih vrsta su i zaštićene i ugrožene vrste koje najčešće pokazuju probleme u razviću semena i reprodukciji. Takođe, ova tehnologija se pokazala kao pogodna za propagaciju i čuvanje različitih drvenastih, lekovitih ili ukrasnih biljaka koje se vegetativno umnožavaju. U radu će biti prikazana metodologija dobijanja veštačkih semena kao i dosadašnji rezultati na primeni tehnologije propagacije vrhova izdanaka hrizantema i ćelijskih suspenzija kestena.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Примена технологије вештачких семена у пропагацији и  конзервацији биљака, Primena tehnologije veštačkih semena u propagaciji i konzervaciji biljaka",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5153"
}

Jevremović, S., Ćalić, D.,& Zdravković-Korać, S.. (2022). Примена технологије вештачких семена у пропагацији и  конзервацији биљака. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society..
https://hdl.handle.net/21.15107/rcub_ibiss_5153

Jevremović S, Ćalić D, Zdravković-Korać S. Примена технологије вештачких семена у пропагацији и  конзервацији биљака. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_5153 .

Jevremović, Slađana, Ćalić, Dušica, Zdravković-Korać, Snežana, "Примена технологије вештачких семена у пропагацији и  конзервацији биљака" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_5153 .

TY  - CONF
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
AU  - Pavlović, Suzana
AU  - Živković, Suzana
AU  - Vinterhalter, Dragan
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5412
AB  - Light and gibberellins (GAs) are essential for the induction of somatic embryogenesis in spinach. In the present study, the influence of light of different spectral qualities on the induction of somatic embryogenesis from the apical root sections of spinach seedlings was tested. The explants grown under blue light (B, emission maximum at 460 nm), provided by Light-emitting diodes (LED), exhibited the highest regeneration frequency (57.8%±0.36) and the mean somatic embryo (SE) number per explant (5.02±0.04) for eight weeks of cultivation on SE-induction medium supplemented containing 20 μM α-naphthaleneacetic acid and 5 μM GA3. The explants cultivated under white (W, 300-720 nm) LED or light provided by fluorescent lamps (FL, 380-680) produced a two times lower number of SEs per explant, while the explants grown under red (R, 630 nm) LED light or in darkness (D) failed to regenerate SEs. Considering that light greatly affects the level of GAs in plant tissues, and GAs are indispensable for SE induction in spinach, the expression profiles of genes encoding the key enzymes that catalyze the final steps of bioactive GA biosynthesis (GA20-oxidase and GA3-oxidase) and inactivation (GA2-oxidase) were tested in the explants grown under B, W or FL light. Surprisingly, no significant differences in the expression levels of these genes were observed among the treatments during of SE induction, indicating that light triggers SE induction by other mechanism, not including alterations in the expression of the aforementioned genes. Further research is needed for better understanding of the mechanism of SE induction by light.
PB  - East Sarajevo : Faculty of Agriculture
PB  - eas
C3  - Book of Abstracts: XII International Scientific Agriculture Symposium "Agrosym 2021"; 2021 Oct 7-10; Jahorina, Bosnia and Herzegovina
T1  - Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)
SP  - 213
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5412
ER  - 

@conference{
author = "Belić, Maja and Zdravković-Korać, Snežana and Pavlović, Suzana and Živković, Suzana and Vinterhalter, Dragan and Ćalić, Dušica and Milojević, Jelena",
year = "2021",
abstract = "Light and gibberellins (GAs) are essential for the induction of somatic embryogenesis in spinach. In the present study, the influence of light of different spectral qualities on the induction of somatic embryogenesis from the apical root sections of spinach seedlings was tested. The explants grown under blue light (B, emission maximum at 460 nm), provided by Light-emitting diodes (LED), exhibited the highest regeneration frequency (57.8%±0.36) and the mean somatic embryo (SE) number per explant (5.02±0.04) for eight weeks of cultivation on SE-induction medium supplemented containing 20 μM α-naphthaleneacetic acid and 5 μM GA3. The explants cultivated under white (W, 300-720 nm) LED or light provided by fluorescent lamps (FL, 380-680) produced a two times lower number of SEs per explant, while the explants grown under red (R, 630 nm) LED light or in darkness (D) failed to regenerate SEs. Considering that light greatly affects the level of GAs in plant tissues, and GAs are indispensable for SE induction in spinach, the expression profiles of genes encoding the key enzymes that catalyze the final steps of bioactive GA biosynthesis (GA20-oxidase and GA3-oxidase) and inactivation (GA2-oxidase) were tested in the explants grown under B, W or FL light. Surprisingly, no significant differences in the expression levels of these genes were observed among the treatments during of SE induction, indicating that light triggers SE induction by other mechanism, not including alterations in the expression of the aforementioned genes. Further research is needed for better understanding of the mechanism of SE induction by light.",
publisher = "East Sarajevo : Faculty of Agriculture, eas",
journal = "Book of Abstracts: XII International Scientific Agriculture Symposium "Agrosym 2021"; 2021 Oct 7-10; Jahorina, Bosnia and Herzegovina",
title = "Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)",
pages = "213",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5412"
}

Belić, M., Zdravković-Korać, S., Pavlović, S., Živković, S., Vinterhalter, D., Ćalić, D.,& Milojević, J.. (2021). Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L). in Book of Abstracts: XII International Scientific Agriculture Symposium "Agrosym 2021"; 2021 Oct 7-10; Jahorina, Bosnia and Herzegovina
East Sarajevo : Faculty of Agriculture., 213.
https://hdl.handle.net/21.15107/rcub_ibiss_5412

Belić M, Zdravković-Korać S, Pavlović S, Živković S, Vinterhalter D, Ćalić D, Milojević J. Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L). in Book of Abstracts: XII International Scientific Agriculture Symposium "Agrosym 2021"; 2021 Oct 7-10; Jahorina, Bosnia and Herzegovina. 2021;:213.
https://hdl.handle.net/21.15107/rcub_ibiss_5412 .

Belić, Maja, Zdravković-Korać, Snežana, Pavlović, Suzana, Živković, Suzana, Vinterhalter, Dragan, Ćalić, Dušica, Milojević, Jelena, "Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)" in Book of Abstracts: XII International Scientific Agriculture Symposium "Agrosym 2021"; 2021 Oct 7-10; Jahorina, Bosnia and Herzegovina (2021):213,
https://hdl.handle.net/21.15107/rcub_ibiss_5412 .

TY  - JOUR
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
AU  - Belić, Maja
AU  - Miletić, Rade
AU  - Zdravković-Korać, Snežana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4319
AB  - Globalization has drastically reduced the number of autochthon apple cultivars in the Serbian market and most of them have nearly disappeared; however, some of these cultivars, such as Petrovača, Budimka, Kolačara Pozna, and Kožara, have extraordinary quality, good pomological characteristics, and pest and disease resistance. The present study was conducted to develop a protocol for the storage of pollen for further use in the conservation and breeding of these cultivars. Viability and germination of the mature pollen were tested in vitro, at four storage temperatures (20, 4, −20, and −80°C), right after harvest or 1, 2, 3, 4, 5, and 6 months after storage. Differences in fresh pollen viability and germination between cultivars were statistically significant and ranged from 60 to 88% and 59 to 98%, respectively. Fresh pollen of cv. Budimka showed the highest viability and germination in comparison with other cultivars, especially cv. Kožara. Pollen viability and germination decreased over the storage period, and it was the lowest after 6 months of storage at room temperature in all tested cultivars. Storage at 4°C prolonged the pollen viability and germinability of 1–5 fold, depending on the cultivar and treatment duration; however, the pollen longevity of all cultivars was significantly extended when stored at −20 or −80°C. After 6 months, pollen of cv. Budimka stored at −20 and −80°C showed 14–15 fold higher germination rates in relation to pollen storage at room temperature for the same period. The results of the present study suggest that the pollen of these apple cultivars could be efficiently maintained at −20°C and could be further used for breeding purposes, e.g., for crossings between cultivars that flower at different times of the year.
PB  - Lausanne: Frontiers Media SA
T2  - Frontiers in Plant Science
T1  - Impact of Storage Temperature on Pollen Viability and Germinability of Four Serbian Autochton Apple Cultivars
VL  - 12
DO  - 10.3389/fpls.2021.709231
SP  - 709231
ER  - 

@article{
author = "Ćalić, Dušica and Milojević, Jelena and Belić, Maja and Miletić, Rade and Zdravković-Korać, Snežana",
year = "2021",
abstract = "Globalization has drastically reduced the number of autochthon apple cultivars in the Serbian market and most of them have nearly disappeared; however, some of these cultivars, such as Petrovača, Budimka, Kolačara Pozna, and Kožara, have extraordinary quality, good pomological characteristics, and pest and disease resistance. The present study was conducted to develop a protocol for the storage of pollen for further use in the conservation and breeding of these cultivars. Viability and germination of the mature pollen were tested in vitro, at four storage temperatures (20, 4, −20, and −80°C), right after harvest or 1, 2, 3, 4, 5, and 6 months after storage. Differences in fresh pollen viability and germination between cultivars were statistically significant and ranged from 60 to 88% and 59 to 98%, respectively. Fresh pollen of cv. Budimka showed the highest viability and germination in comparison with other cultivars, especially cv. Kožara. Pollen viability and germination decreased over the storage period, and it was the lowest after 6 months of storage at room temperature in all tested cultivars. Storage at 4°C prolonged the pollen viability and germinability of 1–5 fold, depending on the cultivar and treatment duration; however, the pollen longevity of all cultivars was significantly extended when stored at −20 or −80°C. After 6 months, pollen of cv. Budimka stored at −20 and −80°C showed 14–15 fold higher germination rates in relation to pollen storage at room temperature for the same period. The results of the present study suggest that the pollen of these apple cultivars could be efficiently maintained at −20°C and could be further used for breeding purposes, e.g., for crossings between cultivars that flower at different times of the year.",
publisher = "Lausanne: Frontiers Media SA",
journal = "Frontiers in Plant Science",
title = "Impact of Storage Temperature on Pollen Viability and Germinability of Four Serbian Autochton Apple Cultivars",
volume = "12",
doi = "10.3389/fpls.2021.709231",
pages = "709231"
}

Ćalić, D., Milojević, J., Belić, M., Miletić, R.,& Zdravković-Korać, S.. (2021). Impact of Storage Temperature on Pollen Viability and Germinability of Four Serbian Autochton Apple Cultivars. in Frontiers in Plant Science
Lausanne: Frontiers Media SA., 12, 709231.
https://doi.org/10.3389/fpls.2021.709231

Ćalić D, Milojević J, Belić M, Miletić R, Zdravković-Korać S. Impact of Storage Temperature on Pollen Viability and Germinability of Four Serbian Autochton Apple Cultivars. in Frontiers in Plant Science. 2021;12:709231.
doi:10.3389/fpls.2021.709231 .

Ćalić, Dušica, Milojević, Jelena, Belić, Maja, Miletić, Rade, Zdravković-Korać, Snežana, "Impact of Storage Temperature on Pollen Viability and Germinability of Four Serbian Autochton Apple Cultivars" in Frontiers in Plant Science, 12 (2021):709231,
https://doi.org/10.3389/fpls.2021.709231 . .

TY  - JOUR
AU  - Devrnja, Nina
AU  - Krstić Milošević, Dijana
AU  - Janošević, Dušica
AU  - Tešević, Vele
AU  - Vinterhalter, Branka
AU  - Savić, Jelena
AU  - Ćalić, Dušica
PY  - 2021
UR  - http://link.springer.com/10.1007/s00709-020-01588-9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4038
AB  - In this study, tansy (Tanacetum vulgare L.) in vitro culture was established from seeds collected from natural populations. The multiplication of plantlets was conducted through shoot tips that exhibited potent apical growth and regeneration capacities on basal medium (BM), without the addition of any plant growth regulators (PGRs). PGRs were also omitted for the establishment and cultivation of tansy root cultures. Both abaxial and adaxial leaf surfaces of in vitro micropropagated plantlets were covered with glandular biseriate trichomes. Histochemical staining showed that glandular secretions were rich in lipid and terpene compounds, confirmed by GC-MS analysis of essential oil (EO). In the total EO, similar portions of oxygenated monoterpenes (38.5% m/m) and oxygenated sesquiterpenes (22.6% m/m) were detected. Chemical profiles of methanol extracts of in vitro cultured tansy shoots and roots varied in quantity and quality from those obtained from wild-growingtansy. HPLC analysis indicated that the methanol extracts of in vitro cultured roots were the richest in 3,5-O-dicaffeoylquinic acid (3,5-O-DCQA), in which the concentration was 6 times higher (10.220 mg/g DW) than that in the extract obtained from roots of wild-growing tansy (1.684 mg/g DW). This result is noticeable in the manner of industrial production of biologically active 3,5-O-DCQA that has been shown to have antioxidant, hepatoprotective, antiviral, antimutagenic, and immunomodulatory activity. Biotechnological interventions on secondary metabolite production taking place in trichomes could further enhance the production of some important tansy metabolites and further investigation will be directed toward the elucidation of the pharmaceutical potential of tansy in vitro obtained metabolites, as mixtures or single moieties.
PB  - Springer
T2  - Protoplasma
T1  - In vitro cultivation of tansy (Tanacetum vulgare L.): a tool for the production of potent pharmaceutical agents
IS  - 3
VL  - 258
DO  - 10.1007/s00709-020-01588-9
SP  - 587
EP  - 599
ER  - 

@article{
author = "Devrnja, Nina and Krstić Milošević, Dijana and Janošević, Dušica and Tešević, Vele and Vinterhalter, Branka and Savić, Jelena and Ćalić, Dušica",
year = "2021",
abstract = "In this study, tansy (Tanacetum vulgare L.) in vitro culture was established from seeds collected from natural populations. The multiplication of plantlets was conducted through shoot tips that exhibited potent apical growth and regeneration capacities on basal medium (BM), without the addition of any plant growth regulators (PGRs). PGRs were also omitted for the establishment and cultivation of tansy root cultures. Both abaxial and adaxial leaf surfaces of in vitro micropropagated plantlets were covered with glandular biseriate trichomes. Histochemical staining showed that glandular secretions were rich in lipid and terpene compounds, confirmed by GC-MS analysis of essential oil (EO). In the total EO, similar portions of oxygenated monoterpenes (38.5% m/m) and oxygenated sesquiterpenes (22.6% m/m) were detected. Chemical profiles of methanol extracts of in vitro cultured tansy shoots and roots varied in quantity and quality from those obtained from wild-growingtansy. HPLC analysis indicated that the methanol extracts of in vitro cultured roots were the richest in 3,5-O-dicaffeoylquinic acid (3,5-O-DCQA), in which the concentration was 6 times higher (10.220 mg/g DW) than that in the extract obtained from roots of wild-growing tansy (1.684 mg/g DW). This result is noticeable in the manner of industrial production of biologically active 3,5-O-DCQA that has been shown to have antioxidant, hepatoprotective, antiviral, antimutagenic, and immunomodulatory activity. Biotechnological interventions on secondary metabolite production taking place in trichomes could further enhance the production of some important tansy metabolites and further investigation will be directed toward the elucidation of the pharmaceutical potential of tansy in vitro obtained metabolites, as mixtures or single moieties.",
publisher = "Springer",
journal = "Protoplasma",
title = "In vitro cultivation of tansy (Tanacetum vulgare L.): a tool for the production of potent pharmaceutical agents",
number = "3",
volume = "258",
doi = "10.1007/s00709-020-01588-9",
pages = "587-599"
}

Devrnja, N., Krstić Milošević, D., Janošević, D., Tešević, V., Vinterhalter, B., Savić, J.,& Ćalić, D.. (2021). In vitro cultivation of tansy (Tanacetum vulgare L.): a tool for the production of potent pharmaceutical agents. in Protoplasma
Springer., 258(3), 587-599.
https://doi.org/10.1007/s00709-020-01588-9

Devrnja N, Krstić Milošević D, Janošević D, Tešević V, Vinterhalter B, Savić J, Ćalić D. In vitro cultivation of tansy (Tanacetum vulgare L.): a tool for the production of potent pharmaceutical agents. in Protoplasma. 2021;258(3):587-599.
doi:10.1007/s00709-020-01588-9 .

Devrnja, Nina, Krstić Milošević, Dijana, Janošević, Dušica, Tešević, Vele, Vinterhalter, Branka, Savić, Jelena, Ćalić, Dušica, "In vitro cultivation of tansy (Tanacetum vulgare L.): a tool for the production of potent pharmaceutical agents" in Protoplasma, 258, no. 3 (2021):587-599,
https://doi.org/10.1007/s00709-020-01588-9 . .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Kostić, Igor
AU  - Milanović, Slobodan
AU  - Šešlija Jovanović, Darka
AU  - Krnjajić, Slobodan
AU  - Ćalić, Dušica
AU  - Stanković, Slađan
AU  - Kostić, Miroslav
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3838
AB  - The Colorado potato beetle, Leptinotarsa decemlineata (Say), is one of the most destructive pest species to have developed resistance to most chemical insecticides. We determined the composition and evaluated the potential of Tanacetum parthenium L. and Tanacetum vulgare L. (Asteraceae family) essential oil (EO) application as an alternative eco-friendly control strategy against L. decemlineata. We assessed the antifeedant activity for L. decemlineata larvae and adults by estimating the damage to potato leaves treated with three concentrations of EOs dissolved in ethanol (0.125, 0.25 and 0.5%). Results showed that T. parthenium EO was more effective against larvae, and T. vulgare was more effective against adults. In an olfactometer assay, the time required to choose an untreated leaf disc did not depend on the Tanacetum species, or life stage examined. However, the concentration of EO exhibited a significant effect on the behaviour of both developmental stages. At higher EO concentrations, both third instar larvae and adults require less time to choose an untreated leaf disc. Additionally, T. parthenium EO provoked more rapid movement away from the treated leaf disc than T. vulgare, especially at the highest concentration. Successful modification of L. decemlineata behaviour by the two Tanacetum oils suggests that they possess the potential for use in potato protection.
PB  - Cambridge University Press
T2  - Bulletin of Entomological Research
T1  - Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say)
IS  - 2
VL  - 11
DO  - 10.1017/S0007485320000504
SP  - 190
EP  - 199
ER  - 

@article{
author = "Lazarević, Jelica and Kostić, Igor and Milanović, Slobodan and Šešlija Jovanović, Darka and Krnjajić, Slobodan and Ćalić, Dušica and Stanković, Slađan and Kostić, Miroslav",
year = "2021",
abstract = "The Colorado potato beetle, Leptinotarsa decemlineata (Say), is one of the most destructive pest species to have developed resistance to most chemical insecticides. We determined the composition and evaluated the potential of Tanacetum parthenium L. and Tanacetum vulgare L. (Asteraceae family) essential oil (EO) application as an alternative eco-friendly control strategy against L. decemlineata. We assessed the antifeedant activity for L. decemlineata larvae and adults by estimating the damage to potato leaves treated with three concentrations of EOs dissolved in ethanol (0.125, 0.25 and 0.5%). Results showed that T. parthenium EO was more effective against larvae, and T. vulgare was more effective against adults. In an olfactometer assay, the time required to choose an untreated leaf disc did not depend on the Tanacetum species, or life stage examined. However, the concentration of EO exhibited a significant effect on the behaviour of both developmental stages. At higher EO concentrations, both third instar larvae and adults require less time to choose an untreated leaf disc. Additionally, T. parthenium EO provoked more rapid movement away from the treated leaf disc than T. vulgare, especially at the highest concentration. Successful modification of L. decemlineata behaviour by the two Tanacetum oils suggests that they possess the potential for use in potato protection.",
publisher = "Cambridge University Press",
journal = "Bulletin of Entomological Research",
title = "Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say)",
number = "2",
volume = "11",
doi = "10.1017/S0007485320000504",
pages = "190-199"
}

Lazarević, J., Kostić, I., Milanović, S., Šešlija Jovanović, D., Krnjajić, S., Ćalić, D., Stanković, S.,& Kostić, M.. (2021). Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say). in Bulletin of Entomological Research
Cambridge University Press., 11(2), 190-199.
https://doi.org/10.1017/S0007485320000504

Lazarević J, Kostić I, Milanović S, Šešlija Jovanović D, Krnjajić S, Ćalić D, Stanković S, Kostić M. Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say). in Bulletin of Entomological Research. 2021;11(2):190-199.
doi:10.1017/S0007485320000504 .

Lazarević, Jelica, Kostić, Igor, Milanović, Slobodan, Šešlija Jovanović, Darka, Krnjajić, Slobodan, Ćalić, Dušica, Stanković, Slađan, Kostić, Miroslav, "Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say)" in Bulletin of Entomological Research, 11, no. 2 (2021):190-199,
https://doi.org/10.1017/S0007485320000504 . .

TY  - GEN
AU  - Ćalić, Dušica
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4332
AB  - Even though plants represent an essential part of our lives offering exploitational, supporting and cultural services, we know very little about the biology of the rarest and most threatened plant species, and even less about their conservation status. Rapid changes in the environment and climate, today more pronounced than ever, affect their fitness and distribution causing rapid species declines, sometimes even before they had been discovered. Despite the high goals set by conservationists to protect native plants from further degradation and extinction, the initiatives for the conservation of threatened species in Europe are scattered and have not yielded the desired results. The main aim of this Action is to improve plant conservation in Europe through the establishment of a network of scientists and other stakeholders who deal with different aspects of plant conservation, from plant taxonomy, ecology, conservation genetics, conservation physiology and reproductive biology to protected area's managers, not forgetting social scientists, who are crucial when dealing with the general public.
PB  - Pensoft Publishers
T2  - Research Ideas and Outcomes
T1  - ConservePlants: An integrated approach to conservation of threatened plants for the 21st Century
VL  - 7
DO  - 10.3897/rio.7.e62810
SP  - e62810
ER  - 

@misc{
author = "Ćalić, Dušica",
year = "2021",
abstract = "Even though plants represent an essential part of our lives offering exploitational, supporting and cultural services, we know very little about the biology of the rarest and most threatened plant species, and even less about their conservation status. Rapid changes in the environment and climate, today more pronounced than ever, affect their fitness and distribution causing rapid species declines, sometimes even before they had been discovered. Despite the high goals set by conservationists to protect native plants from further degradation and extinction, the initiatives for the conservation of threatened species in Europe are scattered and have not yielded the desired results. The main aim of this Action is to improve plant conservation in Europe through the establishment of a network of scientists and other stakeholders who deal with different aspects of plant conservation, from plant taxonomy, ecology, conservation genetics, conservation physiology and reproductive biology to protected area's managers, not forgetting social scientists, who are crucial when dealing with the general public.",
publisher = "Pensoft Publishers",
journal = "Research Ideas and Outcomes",
title = "ConservePlants: An integrated approach to conservation of threatened plants for the 21st Century",
volume = "7",
doi = "10.3897/rio.7.e62810",
pages = "e62810"
}

Ćalić, D.. (2021). ConservePlants: An integrated approach to conservation of threatened plants for the 21st Century. in Research Ideas and Outcomes
Pensoft Publishers., 7, e62810.
https://doi.org/10.3897/rio.7.e62810

Ćalić D. ConservePlants: An integrated approach to conservation of threatened plants for the 21st Century. in Research Ideas and Outcomes. 2021;7:e62810.
doi:10.3897/rio.7.e62810 .

Ćalić, Dušica, "ConservePlants: An integrated approach to conservation of threatened plants for the 21st Century" in Research Ideas and Outcomes, 7 (2021):e62810,
https://doi.org/10.3897/rio.7.e62810 . .

TY  - JOUR
AU  - Devrnja, Nina
AU  - Kostić, Igor
AU  - Lazarević, Jelica
AU  - Savić, Jelena
AU  - Ćalić, Dušica
PY  - 2020
UR  - internal-pdf://Devrnja et al. - 2020 - Evaluation of tansy essential oil as a potential “green” alternative for gypsy moth control.pdf
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3601
AB  - The development of "green" alternatives to chemical pesticides could play a crucial role in integrated pest management (IPM). Their use is considered either as a substitution for or in addition to hazardous synthetic products. We analysed the influence of three concentrations of tansy (Tanacetum vulgare L.) essential oil (EO), previously characterised by GC-MS, on the survival and moulting of the 2nd instar and the nutritional indices of the 4th instar gypsy moth (Lymantria dispar L.) larvae. In a residual contact toxicity assessment, the exposure to tansy EO caused low mortality (< 10%) while larval development was significantly slowed down, i.e., the percentage of larvae that moulted into the 3rd instar was reduced. On the other hand, when tansy EO was incorporated into the diet (digestive toxicity assay), high mortality and a lack of moulting after 120 h of eating were recorded for the highest applied concentration of EO. During 48 h of feeding on EO-supplemented food at concentrations of 0.5 and 1% (v/v), the relative growth rate (RGR) of the 4th instar larvae significantly decreased, which can be explained by a significant reduction of the relative consumption rate (RCR) and significantly or marginally significantly lower efficiency of conversion of ingested food into insect biomass (ECI). Although the RCR was also reduced with the lowest applied EO concentration (0.1%), the ECI was not affected which meant the RGR was as high as it was for the control larvae. ECI changes, when two higher EO concentrations were applied, were due to a reduction in the efficiency of conversion of digested food into biomass (ECD), while approximate digestibility was unaffected by the presence of EO in the food. Our results on the significant negative effects of tansy EO on gypsy moth larval survival, development time, and nutritional physiology suggest that it could be considered in future designs for botanical insecticides for gypsy moth control.
T2  - Environmental Science and Pollution Research
T1  - Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control.
VL  - 27
DO  - 10.1007/s11356-020-07825-1
SP  - 11958
EP  - 11967
ER  - 

@article{
author = "Devrnja, Nina and Kostić, Igor and Lazarević, Jelica and Savić, Jelena and Ćalić, Dušica",
year = "2020",
abstract = "The development of "green" alternatives to chemical pesticides could play a crucial role in integrated pest management (IPM). Their use is considered either as a substitution for or in addition to hazardous synthetic products. We analysed the influence of three concentrations of tansy (Tanacetum vulgare L.) essential oil (EO), previously characterised by GC-MS, on the survival and moulting of the 2nd instar and the nutritional indices of the 4th instar gypsy moth (Lymantria dispar L.) larvae. In a residual contact toxicity assessment, the exposure to tansy EO caused low mortality (< 10%) while larval development was significantly slowed down, i.e., the percentage of larvae that moulted into the 3rd instar was reduced. On the other hand, when tansy EO was incorporated into the diet (digestive toxicity assay), high mortality and a lack of moulting after 120 h of eating were recorded for the highest applied concentration of EO. During 48 h of feeding on EO-supplemented food at concentrations of 0.5 and 1% (v/v), the relative growth rate (RGR) of the 4th instar larvae significantly decreased, which can be explained by a significant reduction of the relative consumption rate (RCR) and significantly or marginally significantly lower efficiency of conversion of ingested food into insect biomass (ECI). Although the RCR was also reduced with the lowest applied EO concentration (0.1%), the ECI was not affected which meant the RGR was as high as it was for the control larvae. ECI changes, when two higher EO concentrations were applied, were due to a reduction in the efficiency of conversion of digested food into biomass (ECD), while approximate digestibility was unaffected by the presence of EO in the food. Our results on the significant negative effects of tansy EO on gypsy moth larval survival, development time, and nutritional physiology suggest that it could be considered in future designs for botanical insecticides for gypsy moth control.",
journal = "Environmental Science and Pollution Research",
title = "Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control.",
volume = "27",
doi = "10.1007/s11356-020-07825-1",
pages = "11958-11967"
}

Devrnja, N., Kostić, I., Lazarević, J., Savić, J.,& Ćalić, D.. (2020). Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control.. in Environmental Science and Pollution Research, 27, 11958-11967.
https://doi.org/10.1007/s11356-020-07825-1

Devrnja N, Kostić I, Lazarević J, Savić J, Ćalić D. Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control.. in Environmental Science and Pollution Research. 2020;27:11958-11967.
doi:10.1007/s11356-020-07825-1 .

Devrnja, Nina, Kostić, Igor, Lazarević, Jelica, Savić, Jelena, Ćalić, Dušica, "Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control." in Environmental Science and Pollution Research, 27 (2020):11958-11967,
https://doi.org/10.1007/s11356-020-07825-1 . .

TY  - JOUR
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
AU  - Uzelac, Branka
AU  - Ćalić, Dušica
AU  - Pavlović, Suzana
AU  - Milojević, Jelena
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3997
AB  - High variability in somatic embryo (SE)-forming capacity has previously been observed in several spinach cultivars. Such variability frequently accounted for more variation in embryogenic response of the explants than the factor being investigated. Hence, the variability in embryogenic capacity was examined in the present study at both the population and the single-seedling level, using seeds of spinach cultivar Matador obtained from nine European seed companies. Seed population obtained from Slovenia (Sl) was superior to others, with the highest regeneration frequency (100%) and the highest mean SE number (14.4). A total of 82% of these seedlings had 80–100% of regenerating explants, while in populations with intermediate embryogenic capacity approximately 40% of seedlings had 20–60% of regenerating explants. The explants from the majority of seedlings (52–100%) in the least responsive populations were irresponsive. Furthermore, the explants from Sl seedlings regenerated from 10–20 (43.5%) up to > 20 (27.6%) SEs on average, while the explants from the majority of seedlings belonging to other populations regenerated 1–10 SEs. The present study strongly indicates that the variability of plant material must not be overlooked, because choosing more responsive individuals for one treatment and less responsive ones for another may lead to misinterpretation of the data.
PB  - Nature Research
T2  - Scientific Reports
T1  - Variability in somatic embryo-forming capacity of spinach
IS  - 1
VL  - 10
DO  - 10.1038/s41598-020-76279-9
SP  - 19290
ER  - 

@article{
author = "Belić, Maja and Zdravković-Korać, Snežana and Uzelac, Branka and Ćalić, Dušica and Pavlović, Suzana and Milojević, Jelena",
year = "2020",
abstract = "High variability in somatic embryo (SE)-forming capacity has previously been observed in several spinach cultivars. Such variability frequently accounted for more variation in embryogenic response of the explants than the factor being investigated. Hence, the variability in embryogenic capacity was examined in the present study at both the population and the single-seedling level, using seeds of spinach cultivar Matador obtained from nine European seed companies. Seed population obtained from Slovenia (Sl) was superior to others, with the highest regeneration frequency (100%) and the highest mean SE number (14.4). A total of 82% of these seedlings had 80–100% of regenerating explants, while in populations with intermediate embryogenic capacity approximately 40% of seedlings had 20–60% of regenerating explants. The explants from the majority of seedlings (52–100%) in the least responsive populations were irresponsive. Furthermore, the explants from Sl seedlings regenerated from 10–20 (43.5%) up to > 20 (27.6%) SEs on average, while the explants from the majority of seedlings belonging to other populations regenerated 1–10 SEs. The present study strongly indicates that the variability of plant material must not be overlooked, because choosing more responsive individuals for one treatment and less responsive ones for another may lead to misinterpretation of the data.",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "Variability in somatic embryo-forming capacity of spinach",
number = "1",
volume = "10",
doi = "10.1038/s41598-020-76279-9",
pages = "19290"
}

Belić, M., Zdravković-Korać, S., Uzelac, B., Ćalić, D., Pavlović, S.,& Milojević, J.. (2020). Variability in somatic embryo-forming capacity of spinach. in Scientific Reports
Nature Research., 10(1), 19290.
https://doi.org/10.1038/s41598-020-76279-9

Belić M, Zdravković-Korać S, Uzelac B, Ćalić D, Pavlović S, Milojević J. Variability in somatic embryo-forming capacity of spinach. in Scientific Reports. 2020;10(1):19290.
doi:10.1038/s41598-020-76279-9 .

Belić, Maja, Zdravković-Korać, Snežana, Uzelac, Branka, Ćalić, Dušica, Pavlović, Suzana, Milojević, Jelena, "Variability in somatic embryo-forming capacity of spinach" in Scientific Reports, 10, no. 1 (2020):19290,
https://doi.org/10.1038/s41598-020-76279-9 . .

TY  - JOUR
AU  - Zdravković-Korać, Snežana
AU  - Tubić, Ljiljana
AU  - Devrnja, Nina
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
AU  - Belić, Maja
AU  - Savić, Jelena
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0304423818308938?via%3Dihub
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0304423818308938
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3224
AB  - Species of the genus Aesculus are among the most attractive ornamental woody plants. Conventional propagation methods of these species are either inefficient (stem cuttings) or unsuitable for clonal propagation (seeds). The aim of the present study was to develop an efficient protocol for clonal propagation of elite specimens of yellow buckeye (Aesculus flava) by somatic embryogenesis. For this purpose, stamen filaments of yellow buckeye were cultivated on media supplemented with 1, 5 or 10 μM 2,4-dichlorophenoxyacetic acid (2,4-D) combined with 0, 1, 5 or 10 μM 6-furfurylaminopurine (Kin), either under light or dark conditions, for 8 weeks, and then subcultivated on plant growth regulator (PGR)-free medium with 400 mg/l of glutamine. The highest somatic embryo (SE) initiation rates were achieved for the explants cultivated in darkness on medium containing 1 μM 2,4-D + 10 μM Kin during callus induction (CI) phase. Embryogenic calli (EC) were initiated from friable calli, starting from the 7th week of culture initiation, while SEs appeared two weeks later, following a week of subcultivation of the explants on PGR-free medium. EC and SEs were observed only in the explants grown in darkness during CI phase. Minimal duration of CI phase and darkness necessary for SE induction was four weeks, while the highest embryogenic response was achieved when each lasted for 8–10 weeks. Obtained SEs were efficiently multiplied on medium supplemented with 0.05 μM 2,4-D + 5 μM Kin by recurrent somatic embryogenesis. SEs at globular stage of development exhibited the highest capacity for secondary SE regeneration. High germination and conversion rates were attained in cotyledonary-stage SEs cultivated on medium with 0.05 μM 2,4-D + 5 μM Kin, but this phase needs to be further optimised, since the obtained plants failed to acclimatize to greenhouse conditions. During the transition of calli from friable to embryogenic state, total peroxidase (POX) activity significantly decreased, indicating their involvement in the acquisition of embryogenic capacity. The presented protocol is suitable for clonal propagation and genetic transformation of this ornamental species, and POX activity may be used as a marker for SE initiation.
T2  - Scientia Horticulturae
T1  - Somatic embryogenesis from stamen filaments of Aesculus flava Sol. and peroxidase activity during the transition from friable to embryogenic callus
VL  - 247
DO  - 10.1016/j.scienta.2018.12.021
SP  - 362
EP  - 372
ER  - 

@article{
author = "Zdravković-Korać, Snežana and Tubić, Ljiljana and Devrnja, Nina and Ćalić, Dušica and Milojević, Jelena and Belić, Maja and Savić, Jelena",
year = "2019",
abstract = "Species of the genus Aesculus are among the most attractive ornamental woody plants. Conventional propagation methods of these species are either inefficient (stem cuttings) or unsuitable for clonal propagation (seeds). The aim of the present study was to develop an efficient protocol for clonal propagation of elite specimens of yellow buckeye (Aesculus flava) by somatic embryogenesis. For this purpose, stamen filaments of yellow buckeye were cultivated on media supplemented with 1, 5 or 10 μM 2,4-dichlorophenoxyacetic acid (2,4-D) combined with 0, 1, 5 or 10 μM 6-furfurylaminopurine (Kin), either under light or dark conditions, for 8 weeks, and then subcultivated on plant growth regulator (PGR)-free medium with 400 mg/l of glutamine. The highest somatic embryo (SE) initiation rates were achieved for the explants cultivated in darkness on medium containing 1 μM 2,4-D + 10 μM Kin during callus induction (CI) phase. Embryogenic calli (EC) were initiated from friable calli, starting from the 7th week of culture initiation, while SEs appeared two weeks later, following a week of subcultivation of the explants on PGR-free medium. EC and SEs were observed only in the explants grown in darkness during CI phase. Minimal duration of CI phase and darkness necessary for SE induction was four weeks, while the highest embryogenic response was achieved when each lasted for 8–10 weeks. Obtained SEs were efficiently multiplied on medium supplemented with 0.05 μM 2,4-D + 5 μM Kin by recurrent somatic embryogenesis. SEs at globular stage of development exhibited the highest capacity for secondary SE regeneration. High germination and conversion rates were attained in cotyledonary-stage SEs cultivated on medium with 0.05 μM 2,4-D + 5 μM Kin, but this phase needs to be further optimised, since the obtained plants failed to acclimatize to greenhouse conditions. During the transition of calli from friable to embryogenic state, total peroxidase (POX) activity significantly decreased, indicating their involvement in the acquisition of embryogenic capacity. The presented protocol is suitable for clonal propagation and genetic transformation of this ornamental species, and POX activity may be used as a marker for SE initiation.",
journal = "Scientia Horticulturae",
title = "Somatic embryogenesis from stamen filaments of Aesculus flava Sol. and peroxidase activity during the transition from friable to embryogenic callus",
volume = "247",
doi = "10.1016/j.scienta.2018.12.021",
pages = "362-372"
}

Zdravković-Korać, S., Tubić, L., Devrnja, N., Ćalić, D., Milojević, J., Belić, M.,& Savić, J.. (2019). Somatic embryogenesis from stamen filaments of Aesculus flava Sol. and peroxidase activity during the transition from friable to embryogenic callus. in Scientia Horticulturae, 247, 362-372.
https://doi.org/10.1016/j.scienta.2018.12.021

Zdravković-Korać S, Tubić L, Devrnja N, Ćalić D, Milojević J, Belić M, Savić J. Somatic embryogenesis from stamen filaments of Aesculus flava Sol. and peroxidase activity during the transition from friable to embryogenic callus. in Scientia Horticulturae. 2019;247:362-372.
doi:10.1016/j.scienta.2018.12.021 .

Zdravković-Korać, Snežana, Tubić, Ljiljana, Devrnja, Nina, Ćalić, Dušica, Milojević, Jelena, Belić, Maja, Savić, Jelena, "Somatic embryogenesis from stamen filaments of Aesculus flava Sol. and peroxidase activity during the transition from friable to embryogenic callus" in Scientia Horticulturae, 247 (2019):362-372,
https://doi.org/10.1016/j.scienta.2018.12.021 . .

TY  - CONF
AU  - Ćalić, Dušica
AU  - Devrnja, Nina
AU  - Milojević, Jelena
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
AU  - Karličić, Vera
AU  - Raičević, Vera
AU  - Ćirković, Saša
AU  - Jasna, Ristić-Đurović
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4330
AB  - Flax (Linum usitatissimum) is an important food and fiber crop, due to its nutritional and nutraceutical qualities. Flax seeds have been consumed for at least 6,000 years, which makes it one of
the world’s first cultivated crops. Seed priming with living bacterial inoculum, termed biopriming,
involves the application of plant growth-promoting rhizobacteria. The present study was conducted to investigate the effect of growth-promoting rhizobacterium Bacillus amyloliquefaciens
on the growth of flax seedlings under different strengths of magnetic field. Seedlings were soaked
in bacterial suspension and bacteria were allowed to colonize. After ten minutes of soaking, the
seedlings were exposed to magnetic field of 60 and 90 mT for 24 h, in dark under laboratory conditions. Untreated seedlings were used as controls. Shoot and root length, number and length of
newly formed lateral roots and biomass production were measured after 1, 3, 5, 7 and 10 days. Results indicate that the combinations of magnetic field and plant growth-promoting bacteria gave
half- to two-fold better results than bacteria or magnetic field alone. The best result was achieved
in seedlings treated with Bacillus amyloliquefaciens and 90 mT magnetic field. This work showed a
significant impact of bio- and magneto-priming on the improvement of flax seedling growth and
biomass productivity.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting)
T1  - Influence of bio- and magneto-priming on flax seedlings growth
SP  - 140
EP  - 141
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4330
ER  - 

@conference{
author = "Ćalić, Dušica and Devrnja, Nina and Milojević, Jelena and Belić, Maja and Zdravković-Korać, Snežana and Karličić, Vera and Raičević, Vera and Ćirković, Saša and Jasna, Ristić-Đurović",
year = "2018",
abstract = "Flax (Linum usitatissimum) is an important food and fiber crop, due to its nutritional and nutraceutical qualities. Flax seeds have been consumed for at least 6,000 years, which makes it one of
the world’s first cultivated crops. Seed priming with living bacterial inoculum, termed biopriming,
involves the application of plant growth-promoting rhizobacteria. The present study was conducted to investigate the effect of growth-promoting rhizobacterium Bacillus amyloliquefaciens
on the growth of flax seedlings under different strengths of magnetic field. Seedlings were soaked
in bacterial suspension and bacteria were allowed to colonize. After ten minutes of soaking, the
seedlings were exposed to magnetic field of 60 and 90 mT for 24 h, in dark under laboratory conditions. Untreated seedlings were used as controls. Shoot and root length, number and length of
newly formed lateral roots and biomass production were measured after 1, 3, 5, 7 and 10 days. Results indicate that the combinations of magnetic field and plant growth-promoting bacteria gave
half- to two-fold better results than bacteria or magnetic field alone. The best result was achieved
in seedlings treated with Bacillus amyloliquefaciens and 90 mT magnetic field. This work showed a
significant impact of bio- and magneto-priming on the improvement of flax seedling growth and
biomass productivity.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)",
title = "Influence of bio- and magneto-priming on flax seedlings growth",
pages = "140-141",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4330"
}

Ćalić, D., Devrnja, N., Milojević, J., Belić, M., Zdravković-Korać, S., Karličić, V., Raičević, V., Ćirković, S.,& Jasna, R.. (2018). Influence of bio- and magneto-priming on flax seedlings growth. in 3rd International Conference on Plant Biology (22nd SPPS Meeting)
Belgrade: Serbian Plant Physiology Society., 140-141.
https://hdl.handle.net/21.15107/rcub_ibiss_4330

Ćalić D, Devrnja N, Milojević J, Belić M, Zdravković-Korać S, Karličić V, Raičević V, Ćirković S, Jasna R. Influence of bio- and magneto-priming on flax seedlings growth. in 3rd International Conference on Plant Biology (22nd SPPS Meeting). 2018;:140-141.
https://hdl.handle.net/21.15107/rcub_ibiss_4330 .

Ćalić, Dušica, Devrnja, Nina, Milojević, Jelena, Belić, Maja, Zdravković-Korać, Snežana, Karličić, Vera, Raičević, Vera, Ćirković, Saša, Jasna, Ristić-Đurović, "Influence of bio- and magneto-priming on flax seedlings growth" in 3rd International Conference on Plant Biology (22nd SPPS Meeting) (2018):140-141,
https://hdl.handle.net/21.15107/rcub_ibiss_4330 .

TY  - CONF
AU  - Zdravković-Korać, Snežana
AU  - Ćalić, Dušica
AU  - Devrnja, Nina
AU  - Belić, Maja
AU  - Milojević, Jelena
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4327
AB  - Mucilage secreted from in vitro-cultured organs has been frequently reported in numerous Allium species. Despite its adverse effect on cell proliferation and subsequent de novo regeneration,
this phenomenon has never been thoroughly studied. In Allium ascalonicum (shallot), only the
uppermost part of the root tip responds to a callus induction treatment by forming callus with
bud-forming capacity. The very same site of the root secretes mucilage during the course of its
cultivation on callus induction medium (CIM). To address this issue, mucilaginous exudate (10 μL),
collected from previously cultured root-tips of shallot, was applied directly to the root-tips isolated
from in vitro-cultured shallot plants. Untreated root-tips were used as a control. All explants were
cultivated on CIM containing 5 μM 2,4 D + 5 μM BA for 10 weeks, and then on regeneration induction medium supplemented with 5 μM BA for 4 weeks. A remarkable difference in cell proliferation
and subsequent bud regeneration between treated and control root-tips were observed. Calli
formed from treated root-tips were 3-fold lighter (53.7 mg vs. 169.9 mg) and regenerated buds
with lower frequency (14.64% vs. 89.2%) and smaller mean bud number per explant (0.1 vs. 15.4),
and these buds were shorter (0.65 mm vs. 16.4 mm) compared to the control. Root-tips isolated
from older roots secreted the mucilage at the higher frequency, formed smaller calli and regenerated fewer buds than those isolated from younger roots. Inhibition of cell proliferation indicates
the potential of this exudate in control of cell proliferation in the treatment of some diseases.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting)
T1  - Mucilaginous exudate from the apical root sections of Allium ascalonicum provokes autoinhibition of proliferation and subsequent bud regeneration
SP  - 38
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4327
ER  - 

@conference{
author = "Zdravković-Korać, Snežana and Ćalić, Dušica and Devrnja, Nina and Belić, Maja and Milojević, Jelena",
year = "2018",
abstract = "Mucilage secreted from in vitro-cultured organs has been frequently reported in numerous Allium species. Despite its adverse effect on cell proliferation and subsequent de novo regeneration,
this phenomenon has never been thoroughly studied. In Allium ascalonicum (shallot), only the
uppermost part of the root tip responds to a callus induction treatment by forming callus with
bud-forming capacity. The very same site of the root secretes mucilage during the course of its
cultivation on callus induction medium (CIM). To address this issue, mucilaginous exudate (10 μL),
collected from previously cultured root-tips of shallot, was applied directly to the root-tips isolated
from in vitro-cultured shallot plants. Untreated root-tips were used as a control. All explants were
cultivated on CIM containing 5 μM 2,4 D + 5 μM BA for 10 weeks, and then on regeneration induction medium supplemented with 5 μM BA for 4 weeks. A remarkable difference in cell proliferation
and subsequent bud regeneration between treated and control root-tips were observed. Calli
formed from treated root-tips were 3-fold lighter (53.7 mg vs. 169.9 mg) and regenerated buds
with lower frequency (14.64% vs. 89.2%) and smaller mean bud number per explant (0.1 vs. 15.4),
and these buds were shorter (0.65 mm vs. 16.4 mm) compared to the control. Root-tips isolated
from older roots secreted the mucilage at the higher frequency, formed smaller calli and regenerated fewer buds than those isolated from younger roots. Inhibition of cell proliferation indicates
the potential of this exudate in control of cell proliferation in the treatment of some diseases.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)",
title = "Mucilaginous exudate from the apical root sections of Allium ascalonicum provokes autoinhibition of proliferation and subsequent bud regeneration",
pages = "38",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4327"
}

Zdravković-Korać, S., Ćalić, D., Devrnja, N., Belić, M.,& Milojević, J.. (2018). Mucilaginous exudate from the apical root sections of Allium ascalonicum provokes autoinhibition of proliferation and subsequent bud regeneration. in 3rd International Conference on Plant Biology (22nd SPPS Meeting)
Belgrade: Serbian Plant Physiology Society., 38.
https://hdl.handle.net/21.15107/rcub_ibiss_4327

Zdravković-Korać S, Ćalić D, Devrnja N, Belić M, Milojević J. Mucilaginous exudate from the apical root sections of Allium ascalonicum provokes autoinhibition of proliferation and subsequent bud regeneration. in 3rd International Conference on Plant Biology (22nd SPPS Meeting). 2018;:38.
https://hdl.handle.net/21.15107/rcub_ibiss_4327 .

Zdravković-Korać, Snežana, Ćalić, Dušica, Devrnja, Nina, Belić, Maja, Milojević, Jelena, "Mucilaginous exudate from the apical root sections of Allium ascalonicum provokes autoinhibition of proliferation and subsequent bud regeneration" in 3rd International Conference on Plant Biology (22nd SPPS Meeting) (2018):38,
https://hdl.handle.net/21.15107/rcub_ibiss_4327 .

TY  - CONF
AU  - Devrnja, Nina
AU  - Kostić, Igor
AU  - Lazarević, Jelica
AU  - Kostić, Miroslav
AU  - Savić, Jelena
AU  - Belić, Maja
AU  - Ćosić, Tatjana
AU  - Ćalić, Dušica
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4326
AB  - Larvae of gypsy moth (Lymantria dispar L.) are major defoliators of deciduous forests and urban
environment. The biological means of regulation of moth population density were incorporated
along with conventional treatment in the integrative management concept. The effects of tansy
(Tanacetum vulgare L.) essential oil (EO) to gypsy moths were monitored through the effects of
residual contact and digestive toxicity on the mortality and development in the second instar
larvae, but also through the growth and feeding indices of the fourth instar larvae. Although EO
presence didn’t cause residual contact toxicity or significantly affect larval mortality, tested oil notably
elongated developmental time by delaying the molting moment and reduced percentage
of molted larvae. On the other hand, EO digestion caused significant mortality in the second instar
larvae (72% compared to control, at the end of experiment). Digestion of food enriched with tansy
oil decreased the efficiency of the diet, and led to reduced mass gain, and therefore induced the
delay of the molting moment, or completely stopped molting into the third instar larvae. Besides
that, EO reduced the daily mass gain (0.06 mg mg-1 day-1 compared to 0.3 mg mg-1 day-1 in control)
and the food consumption rate (0.61 mg mg-1 day-1 compared to 1.23 mg mg-1 day-1 in control)
in the fourth instar larvae and caused decrease of the conversion rate of digested food to larvae
body mass (10.62% compared to 26.05% in control). Despite that, food assimilation rate to larvae
body mass was not significantly decreased, which moderated negative effects of EO digestion as
well as reduced food intake.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts
T1  - Effects of tansy essential oil on fitness and digestion process of gypsy moth larvae
SP  - 136
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4326
ER  - 

@conference{
author = "Devrnja, Nina and Kostić, Igor and Lazarević, Jelica and Kostić, Miroslav and Savić, Jelena and Belić, Maja and Ćosić, Tatjana and Ćalić, Dušica",
year = "2018",
abstract = "Larvae of gypsy moth (Lymantria dispar L.) are major defoliators of deciduous forests and urban
environment. The biological means of regulation of moth population density were incorporated
along with conventional treatment in the integrative management concept. The effects of tansy
(Tanacetum vulgare L.) essential oil (EO) to gypsy moths were monitored through the effects of
residual contact and digestive toxicity on the mortality and development in the second instar
larvae, but also through the growth and feeding indices of the fourth instar larvae. Although EO
presence didn’t cause residual contact toxicity or significantly affect larval mortality, tested oil notably
elongated developmental time by delaying the molting moment and reduced percentage
of molted larvae. On the other hand, EO digestion caused significant mortality in the second instar
larvae (72% compared to control, at the end of experiment). Digestion of food enriched with tansy
oil decreased the efficiency of the diet, and led to reduced mass gain, and therefore induced the
delay of the molting moment, or completely stopped molting into the third instar larvae. Besides
that, EO reduced the daily mass gain (0.06 mg mg-1 day-1 compared to 0.3 mg mg-1 day-1 in control)
and the food consumption rate (0.61 mg mg-1 day-1 compared to 1.23 mg mg-1 day-1 in control)
in the fourth instar larvae and caused decrease of the conversion rate of digested food to larvae
body mass (10.62% compared to 26.05% in control). Despite that, food assimilation rate to larvae
body mass was not significantly decreased, which moderated negative effects of EO digestion as
well as reduced food intake.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts",
title = "Effects of tansy essential oil on fitness and digestion process of gypsy moth larvae",
pages = "136",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4326"
}

Devrnja, N., Kostić, I., Lazarević, J., Kostić, M., Savić, J., Belić, M., Ćosić, T.,& Ćalić, D.. (2018). Effects of tansy essential oil on fitness and digestion process of gypsy moth larvae. in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts
Belgrade: Serbian Plant Physiology Society., 136.
https://hdl.handle.net/21.15107/rcub_ibiss_4326

Devrnja N, Kostić I, Lazarević J, Kostić M, Savić J, Belić M, Ćosić T, Ćalić D. Effects of tansy essential oil on fitness and digestion process of gypsy moth larvae. in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts. 2018;:136.
https://hdl.handle.net/21.15107/rcub_ibiss_4326 .

Devrnja, Nina, Kostić, Igor, Lazarević, Jelica, Kostić, Miroslav, Savić, Jelena, Belić, Maja, Ćosić, Tatjana, Ćalić, Dušica, "Effects of tansy essential oil on fitness and digestion process of gypsy moth larvae" in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts (2018):136,
https://hdl.handle.net/21.15107/rcub_ibiss_4326 .

TY  - CONF
AU  - Belić, Maja
AU  - Anačkov, Goran
AU  - Ćalić, Dušica
AU  - Devrnja, Nina
AU  - Igić, Ružica
AU  - Milojević, Jelena
AU  - Zdravković-Korać, Snežana
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4325
AB  - A. atropurpureum is a natural rarity of Serbia, growing only in the area of Vojvodina. The vulnerability of its habitat by fragmentation and frequent and uncontrolled chemical treatment of
surrounding agrarian areas, affects its status and the impoverishment of natural populations.
Therefore, establishing a protocol for efficient de novo regeneration of this species for ex situ conservation was the aim of the present study. For callus induction, the apical root sections of axenic
seedlings were cultivated on medium supplemented with 5 μM 2,4 D + 5 μM BA for 8 weeks. The
obtained calli were friable, pale beige, without regeneration capacity. However, within these calli, a
compact yellowish callus formed, and this type of callus had the capacity for bud formation when
cultivated on media containing 0, 1, 5 or 10 μM TDZ, Kin or BA for 8 weeks. Calli cultivated on medium supplemented with 10 μM Kin exhibited the highest bud forming capacity, with the lowest
level of hyperhydricity and albinism. Shoot bunches were further hardened on plant growth regulator-free medium for 8 weeks, and then single plants were detached and subcultivated on media
with 0, 1, 5 or 10 μM GA3 and grown at 12 °C, for bulblet induction. All plants formed bulblets, but
bulblets cultivated on GA3-containing media multiplied by setting up to 10 secondary bulblets.
Obtained bulblets are suitable propagules, as they easily develop into plants. This protocol is convenient for clonal propagation of this endangered plant species, as well as for other applications
in its research and improvement.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting)
T1  - Bud regeneration from root-tips of Allium atropurpureum Waldst. & Kit.
SP  - 30
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4325
ER  - 

@conference{
author = "Belić, Maja and Anačkov, Goran and Ćalić, Dušica and Devrnja, Nina and Igić, Ružica and Milojević, Jelena and Zdravković-Korać, Snežana",
year = "2018",
abstract = "A. atropurpureum is a natural rarity of Serbia, growing only in the area of Vojvodina. The vulnerability of its habitat by fragmentation and frequent and uncontrolled chemical treatment of
surrounding agrarian areas, affects its status and the impoverishment of natural populations.
Therefore, establishing a protocol for efficient de novo regeneration of this species for ex situ conservation was the aim of the present study. For callus induction, the apical root sections of axenic
seedlings were cultivated on medium supplemented with 5 μM 2,4 D + 5 μM BA for 8 weeks. The
obtained calli were friable, pale beige, without regeneration capacity. However, within these calli, a
compact yellowish callus formed, and this type of callus had the capacity for bud formation when
cultivated on media containing 0, 1, 5 or 10 μM TDZ, Kin or BA for 8 weeks. Calli cultivated on medium supplemented with 10 μM Kin exhibited the highest bud forming capacity, with the lowest
level of hyperhydricity and albinism. Shoot bunches were further hardened on plant growth regulator-free medium for 8 weeks, and then single plants were detached and subcultivated on media
with 0, 1, 5 or 10 μM GA3 and grown at 12 °C, for bulblet induction. All plants formed bulblets, but
bulblets cultivated on GA3-containing media multiplied by setting up to 10 secondary bulblets.
Obtained bulblets are suitable propagules, as they easily develop into plants. This protocol is convenient for clonal propagation of this endangered plant species, as well as for other applications
in its research and improvement.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)",
title = "Bud regeneration from root-tips of Allium atropurpureum Waldst. & Kit.",
pages = "30",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4325"
}

Belić, M., Anačkov, G., Ćalić, D., Devrnja, N., Igić, R., Milojević, J.,& Zdravković-Korać, S.. (2018). Bud regeneration from root-tips of Allium atropurpureum Waldst. & Kit.. in 3rd International Conference on Plant Biology (22nd SPPS Meeting)
Belgrade: Serbian Plant Physiology Society., 30.
https://hdl.handle.net/21.15107/rcub_ibiss_4325

Belić M, Anačkov G, Ćalić D, Devrnja N, Igić R, Milojević J, Zdravković-Korać S. Bud regeneration from root-tips of Allium atropurpureum Waldst. & Kit.. in 3rd International Conference on Plant Biology (22nd SPPS Meeting). 2018;:30.
https://hdl.handle.net/21.15107/rcub_ibiss_4325 .

Belić, Maja, Anačkov, Goran, Ćalić, Dušica, Devrnja, Nina, Igić, Ružica, Milojević, Jelena, Zdravković-Korać, Snežana, "Bud regeneration from root-tips of Allium atropurpureum Waldst. & Kit." in 3rd International Conference on Plant Biology (22nd SPPS Meeting) (2018):30,
https://hdl.handle.net/21.15107/rcub_ibiss_4325 .

TY  - CONF
AU  - Belić, Maja
AU  - Devrnja, Nina
AU  - Ćalić, Dušica
AU  - Zdravković-Korać, Snežana
AU  - Milojević, Jelena
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4324
AB  - Gibberellins (GA) are essential for induction of somatic embryogenesis from spinach apical root
sections. To elucidate the role of GA in this process, expression of genes encoding enzymes that
catalyze the final step of GA20 oxidation to bioactive GA, GA3-oxidase (GA3-ox), and GA degradation, GA2-oxidase (GA2-ox), was assessed. In spinach, there is only one GA3-ox and three GA2-ox
(GA2ox 1, GA2 ox2, GA2 ox3). Expression of these genes was tested in the explants cultivated on
noninductive medium (NM), supplemented with 20 μM NAA, inductive medium (IM), containing
20 μM NAA + 5 μM GA3, or plant growth regulator (PGR)-free medium during a 28 d induction
period. Root-tips isolated from seedlings and immediately frozen for RNA isolation were used as a
control. In the explants cultivated on PGR-free medium, expression of GA3-ox increased gradually
up to 10-fold and was constantly higher than in control. GA2-ox1, with an increase of up to 60-fold, was the most highly expressed GA2-ox, while the expression of GA2-ox2 and GA2-ox3 only
slightly increased compared to the control. In the explants cultivated on NM, expression of GA3-ox
decreased slightly until the 7th day of cultivation and then increased up to 2-fold until the end
of the experiment, while the expression of GA2-ox1 and GA2-ox2 was only slightly higher than
in control. However, explants cultivated on IM showed the constant and significant decrease of
GA3-ox (down to 7-fold) and increase of GA2-ox2 (up to 20-fold) expression. Here, we propose that
continuous decrease in GA3-ox and increase in GA2-ox2 expression were favorable conditions for
somatic embryo induction.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting)
T1  - Enhanced gibberellin catabolism promotes somatic embryo induction from spinach apical root sections
SP  - 29
EP  - 30
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4324
ER  - 

@conference{
author = "Belić, Maja and Devrnja, Nina and Ćalić, Dušica and Zdravković-Korać, Snežana and Milojević, Jelena",
year = "2018",
abstract = "Gibberellins (GA) are essential for induction of somatic embryogenesis from spinach apical root
sections. To elucidate the role of GA in this process, expression of genes encoding enzymes that
catalyze the final step of GA20 oxidation to bioactive GA, GA3-oxidase (GA3-ox), and GA degradation, GA2-oxidase (GA2-ox), was assessed. In spinach, there is only one GA3-ox and three GA2-ox
(GA2ox 1, GA2 ox2, GA2 ox3). Expression of these genes was tested in the explants cultivated on
noninductive medium (NM), supplemented with 20 μM NAA, inductive medium (IM), containing
20 μM NAA + 5 μM GA3, or plant growth regulator (PGR)-free medium during a 28 d induction
period. Root-tips isolated from seedlings and immediately frozen for RNA isolation were used as a
control. In the explants cultivated on PGR-free medium, expression of GA3-ox increased gradually
up to 10-fold and was constantly higher than in control. GA2-ox1, with an increase of up to 60-fold, was the most highly expressed GA2-ox, while the expression of GA2-ox2 and GA2-ox3 only
slightly increased compared to the control. In the explants cultivated on NM, expression of GA3-ox
decreased slightly until the 7th day of cultivation and then increased up to 2-fold until the end
of the experiment, while the expression of GA2-ox1 and GA2-ox2 was only slightly higher than
in control. However, explants cultivated on IM showed the constant and significant decrease of
GA3-ox (down to 7-fold) and increase of GA2-ox2 (up to 20-fold) expression. Here, we propose that
continuous decrease in GA3-ox and increase in GA2-ox2 expression were favorable conditions for
somatic embryo induction.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)",
title = "Enhanced gibberellin catabolism promotes somatic embryo induction from spinach apical root sections",
pages = "29-30",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4324"
}

Belić, M., Devrnja, N., Ćalić, D., Zdravković-Korać, S.,& Milojević, J.. (2018). Enhanced gibberellin catabolism promotes somatic embryo induction from spinach apical root sections. in 3rd International Conference on Plant Biology (22nd SPPS Meeting)
Belgrade: Serbian Plant Physiology Society., 29-30.
https://hdl.handle.net/21.15107/rcub_ibiss_4324

Belić M, Devrnja N, Ćalić D, Zdravković-Korać S, Milojević J. Enhanced gibberellin catabolism promotes somatic embryo induction from spinach apical root sections. in 3rd International Conference on Plant Biology (22nd SPPS Meeting). 2018;:29-30.
https://hdl.handle.net/21.15107/rcub_ibiss_4324 .

Belić, Maja, Devrnja, Nina, Ćalić, Dušica, Zdravković-Korać, Snežana, Milojević, Jelena, "Enhanced gibberellin catabolism promotes somatic embryo induction from spinach apical root sections" in 3rd International Conference on Plant Biology (22nd SPPS Meeting) (2018):29-30,
https://hdl.handle.net/21.15107/rcub_ibiss_4324 .

TY  - CONF
AU  - Zdravković-Korać, Snežana
AU  - Belić, Maja
AU  - Devrnja, Nina
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4322
AB  - The apical root sections of Allium ascalonicum plants respond to a callus inducing treatment by
forming callus from the root apical meristem. It has been shown in Arabidopsis thaliana that balance between superoxide and hydrogen peroxide (H2O2) in the root tip determines root meristem
size by controlling the transition from cell proliferation to differentiation. Hence, the increased size
of root meristem may affect callus formation and subsequent bud regeneration from the root-tips.
To test this hypothesis, in vitro-grown shallot plants were pretreated with aqueous solutions of
H2O2 (100, 500 or 1000 μM) or potassium iodide (KI, 1 or 10 mM) for 24h. Plants incubated in water
were used as a control. The apical root sections (1 cm) isolated from these plants were cultivated
on callus induction medium, supplemented with 5 μM 2,4-D + 5 μM BA, for 8 weeks and then on
regeneration induction medium, containing 5 μM BA, for 4 weeks. Obtained results indicate the
significant impact of KI on de novo bud regeneration. Explants treated with 1 mM KI regenerated
buds with significantly higher frequency (83.6% vs. 25.2%) and the mean bud number (6.19 vs.
0.21) compared to the control. The frequencies of bud regeneration and the mean bud numbers obtained for H2O2-treated explants were significantly lower compared to KI-treated explants
(43.5% 52.0% and 1.0-2.3, respectively). Assuming that H2O2 decreases size of the root meristem,
as was shown in Arabidopsis, the results obtained in this work suggest that KI, a H2O2 scavenger,
increased bud-forming capacity presumably by increasing the size of root meristem.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting)
T1  - Potassium iodide promotes bud regeneration from the apical root sections of shallot plants
SP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4322
ER  - 

@conference{
author = "Zdravković-Korać, Snežana and Belić, Maja and Devrnja, Nina and Ćalić, Dušica and Milojević, Jelena",
year = "2018",
abstract = "The apical root sections of Allium ascalonicum plants respond to a callus inducing treatment by
forming callus from the root apical meristem. It has been shown in Arabidopsis thaliana that balance between superoxide and hydrogen peroxide (H2O2) in the root tip determines root meristem
size by controlling the transition from cell proliferation to differentiation. Hence, the increased size
of root meristem may affect callus formation and subsequent bud regeneration from the root-tips.
To test this hypothesis, in vitro-grown shallot plants were pretreated with aqueous solutions of
H2O2 (100, 500 or 1000 μM) or potassium iodide (KI, 1 or 10 mM) for 24h. Plants incubated in water
were used as a control. The apical root sections (1 cm) isolated from these plants were cultivated
on callus induction medium, supplemented with 5 μM 2,4-D + 5 μM BA, for 8 weeks and then on
regeneration induction medium, containing 5 μM BA, for 4 weeks. Obtained results indicate the
significant impact of KI on de novo bud regeneration. Explants treated with 1 mM KI regenerated
buds with significantly higher frequency (83.6% vs. 25.2%) and the mean bud number (6.19 vs.
0.21) compared to the control. The frequencies of bud regeneration and the mean bud numbers obtained for H2O2-treated explants were significantly lower compared to KI-treated explants
(43.5% 52.0% and 1.0-2.3, respectively). Assuming that H2O2 decreases size of the root meristem,
as was shown in Arabidopsis, the results obtained in this work suggest that KI, a H2O2 scavenger,
increased bud-forming capacity presumably by increasing the size of root meristem.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)",
title = "Potassium iodide promotes bud regeneration from the apical root sections of shallot plants",
pages = "39",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4322"
}

Zdravković-Korać, S., Belić, M., Devrnja, N., Ćalić, D.,& Milojević, J.. (2018). Potassium iodide promotes bud regeneration from the apical root sections of shallot plants. in 3rd International Conference on Plant Biology (22nd SPPS Meeting)
Belgrade: Serbian Plant Physiology Society., 39.
https://hdl.handle.net/21.15107/rcub_ibiss_4322

Zdravković-Korać S, Belić M, Devrnja N, Ćalić D, Milojević J. Potassium iodide promotes bud regeneration from the apical root sections of shallot plants. in 3rd International Conference on Plant Biology (22nd SPPS Meeting). 2018;:39.
https://hdl.handle.net/21.15107/rcub_ibiss_4322 .

Zdravković-Korać, Snežana, Belić, Maja, Devrnja, Nina, Ćalić, Dušica, Milojević, Jelena, "Potassium iodide promotes bud regeneration from the apical root sections of shallot plants" in 3rd International Conference on Plant Biology (22nd SPPS Meeting) (2018):39,
https://hdl.handle.net/21.15107/rcub_ibiss_4322 .

TY  - CONF
AU  - Ćalić, Dušica
AU  - Todorović, Dajana
AU  - Petković, Branka
AU  - Devrnja, Nina
AU  - Milojević, Jelena
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4015
AB  - In the context of seed technology, the use of magneto-priming, as a method for increasing
plant production, offers advantages in comparison to conventional treatments with chemical substances.
Magneto-priming is a cheap, non-invasive technique, based on the application of magnetic
fields (MF) and described as eco-friendly, with proved beneficial effects on seed germination
and subsequent plant biomass yield. In this study, seeds of flax (Linum usitatissimum), placed on
moist filter-paper, were treated with 100 mT MF strength, with exposure time of 0, 24, 48, 72, 96
and 120 h and kept in dark under laboratory conditions. Seeds that were not exposed to magnetic
field were used as controls. Efficacy of MF on seed germination and seedlings height, shoot and
root length and biomass was evaluated. The MF promoted seed germination rate by 10%-40 %,
depending of treatments, compared to control. The growth parameters were better in seedlings
exposed to all magnetic treatments, with an increase of up to 60% over the control. MF treatment
of 24 h had a 50% better effect than 120 h MF treatment. In addition, the best biomass yield of
acclimated plants was achieved after the shortest magnetic exposure treatment. This study suggests
a positive effect of stable MF on flax seed germination and subsequent growth of seedlings.
Therefore, magnetic field may be useful in organic agriculture in replacing the usage of plant
growth regulators.
PB  - Serbian Plant Physiology Society
PB  - Institute for Biological Research “Siniša Stanković”, University of Belgrade
PB  - Faculty of Biology, University of Belgrade
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting)
T1  - Effect of seed magneto-priming on flax (Linum usitatissimum) seed germination and subsequent seedling growth
SP  - 141
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4015
ER  - 

@conference{
author = "Ćalić, Dušica and Todorović, Dajana and Petković, Branka and Devrnja, Nina and Milojević, Jelena and Belić, Maja and Zdravković-Korać, Snežana",
year = "2018",
abstract = "In the context of seed technology, the use of magneto-priming, as a method for increasing
plant production, offers advantages in comparison to conventional treatments with chemical substances.
Magneto-priming is a cheap, non-invasive technique, based on the application of magnetic
fields (MF) and described as eco-friendly, with proved beneficial effects on seed germination
and subsequent plant biomass yield. In this study, seeds of flax (Linum usitatissimum), placed on
moist filter-paper, were treated with 100 mT MF strength, with exposure time of 0, 24, 48, 72, 96
and 120 h and kept in dark under laboratory conditions. Seeds that were not exposed to magnetic
field were used as controls. Efficacy of MF on seed germination and seedlings height, shoot and
root length and biomass was evaluated. The MF promoted seed germination rate by 10%-40 %,
depending of treatments, compared to control. The growth parameters were better in seedlings
exposed to all magnetic treatments, with an increase of up to 60% over the control. MF treatment
of 24 h had a 50% better effect than 120 h MF treatment. In addition, the best biomass yield of
acclimated plants was achieved after the shortest magnetic exposure treatment. This study suggests
a positive effect of stable MF on flax seed germination and subsequent growth of seedlings.
Therefore, magnetic field may be useful in organic agriculture in replacing the usage of plant
growth regulators.",
publisher = "Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Faculty of Biology, University of Belgrade",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)",
title = "Effect of seed magneto-priming on flax (Linum usitatissimum) seed germination and subsequent seedling growth",
pages = "141",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4015"
}

Ćalić, D., Todorović, D., Petković, B., Devrnja, N., Milojević, J., Belić, M.,& Zdravković-Korać, S.. (2018). Effect of seed magneto-priming on flax (Linum usitatissimum) seed germination and subsequent seedling growth. in 3rd International Conference on Plant Biology (22nd SPPS Meeting)
Serbian Plant Physiology Society., 141.
https://hdl.handle.net/21.15107/rcub_ibiss_4015

Ćalić D, Todorović D, Petković B, Devrnja N, Milojević J, Belić M, Zdravković-Korać S. Effect of seed magneto-priming on flax (Linum usitatissimum) seed germination and subsequent seedling growth. in 3rd International Conference on Plant Biology (22nd SPPS Meeting). 2018;:141.
https://hdl.handle.net/21.15107/rcub_ibiss_4015 .

Ćalić, Dušica, Todorović, Dajana, Petković, Branka, Devrnja, Nina, Milojević, Jelena, Belić, Maja, Zdravković-Korać, Snežana, "Effect of seed magneto-priming on flax (Linum usitatissimum) seed germination and subsequent seedling growth" in 3rd International Conference on Plant Biology (22nd SPPS Meeting) (2018):141,
https://hdl.handle.net/21.15107/rcub_ibiss_4015 .