TY  - CONF
AU  - Milošević, Verica
AU  - Filipović, Branko
AU  - Živanović, Jasmina
AU  - Miler, Marko
AU  - Manojlović-Stojanoski, Milica
AU  - Ušćebrka, gordana
AU  - Ajdžanović, Vladimir
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5927
AB  - In traditional medicine, the Mediterranean plant Vitex after the last injection. Pituitary glands were excised, agnus-castus L., Familia Verbenacee (Vac) has been weighed and processed for the stereological analysis. used since ancient times to alleviate the symptoms of ACTH-, TSH- and PRL-producing cells were identified with aging. We have already shown that the essential oil of polyclonal rabbit antisera (ACTH, TSHß, PRL respectively).
Vac stimulates thyroid C cells activity and decreases bone ACTH and TSH blood levels were determined using the turnover in middle-aged male rats [1). Herein, essential immunoassays. The stereological analysis, after treatment oil isolated by hydrodistillation from ripe Vac barriers was with Vac, showed that the immunopositive ACTH and applied to middle aged male rats with aim to evaluate its PRL cells had decreased (p<0.05) relative volume density effects on the pituitary hormone producing cells. Animals by 18 and 36% respectively, while the same parameter were divided into two groups. The first group was treated was significantly increased by 73% in TSH cells, all in subcutaneously with Vac essential oil (60 mg/kg b.m.), comparison with the values obtained for the controls. once a day for 3 weeks, while the second one was treated
Circulating concentration of ACTH decreased (p<0.05) by
with sterile olive oil by the same schedule, and served as 52%, while the level of TSH increased (p<0.01) for 45%, the control. The administered dose of Vac essential oil had all compared to the adequate parameters in control rats. bone-protective and sedative effects, as well as analgesic These results show that Vac essential oil differently affects effect in our and other researchers' studies, with absolute the stereological parameters and hormonal output of rate of survival [1, 2]. Decapitatation was performed 24h various pituitary cells.
PB  - Saint-Petersburg: Eco-Vector
C3  - 23th International Congress Phytopharm; 2019 Jul 1-3; Saint-Petersburg, Russia
T1  - The effects of Vitex agnus castus L. essential oil on the pituitary cells in middle aged rats
SP  - 40
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5927
ER  - 

@conference{
author = "Milošević, Verica and Filipović, Branko and Živanović, Jasmina and Miler, Marko and Manojlović-Stojanoski, Milica and Ušćebrka, gordana and Ajdžanović, Vladimir",
year = "2019",
abstract = "In traditional medicine, the Mediterranean plant Vitex after the last injection. Pituitary glands were excised, agnus-castus L., Familia Verbenacee (Vac) has been weighed and processed for the stereological analysis. used since ancient times to alleviate the symptoms of ACTH-, TSH- and PRL-producing cells were identified with aging. We have already shown that the essential oil of polyclonal rabbit antisera (ACTH, TSHß, PRL respectively).
Vac stimulates thyroid C cells activity and decreases bone ACTH and TSH blood levels were determined using the turnover in middle-aged male rats [1). Herein, essential immunoassays. The stereological analysis, after treatment oil isolated by hydrodistillation from ripe Vac barriers was with Vac, showed that the immunopositive ACTH and applied to middle aged male rats with aim to evaluate its PRL cells had decreased (p<0.05) relative volume density effects on the pituitary hormone producing cells. Animals by 18 and 36% respectively, while the same parameter were divided into two groups. The first group was treated was significantly increased by 73% in TSH cells, all in subcutaneously with Vac essential oil (60 mg/kg b.m.), comparison with the values obtained for the controls. once a day for 3 weeks, while the second one was treated
Circulating concentration of ACTH decreased (p<0.05) by
with sterile olive oil by the same schedule, and served as 52%, while the level of TSH increased (p<0.01) for 45%, the control. The administered dose of Vac essential oil had all compared to the adequate parameters in control rats. bone-protective and sedative effects, as well as analgesic These results show that Vac essential oil differently affects effect in our and other researchers' studies, with absolute the stereological parameters and hormonal output of rate of survival [1, 2]. Decapitatation was performed 24h various pituitary cells.",
publisher = "Saint-Petersburg: Eco-Vector",
journal = "23th International Congress Phytopharm; 2019 Jul 1-3; Saint-Petersburg, Russia",
title = "The effects of Vitex agnus castus L. essential oil on the pituitary cells in middle aged rats",
pages = "40",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5927"
}

Milošević, V., Filipović, B., Živanović, J., Miler, M., Manojlović-Stojanoski, M., Ušćebrka, g.,& Ajdžanović, V.. (2019). The effects of Vitex agnus castus L. essential oil on the pituitary cells in middle aged rats. in 23th International Congress Phytopharm; 2019 Jul 1-3; Saint-Petersburg, Russia
Saint-Petersburg: Eco-Vector., 40.
https://hdl.handle.net/21.15107/rcub_ibiss_5927

Milošević V, Filipović B, Živanović J, Miler M, Manojlović-Stojanoski M, Ušćebrka G, Ajdžanović V. The effects of Vitex agnus castus L. essential oil on the pituitary cells in middle aged rats. in 23th International Congress Phytopharm; 2019 Jul 1-3; Saint-Petersburg, Russia. 2019;:40.
https://hdl.handle.net/21.15107/rcub_ibiss_5927 .

Milošević, Verica, Filipović, Branko, Živanović, Jasmina, Miler, Marko, Manojlović-Stojanoski, Milica, Ušćebrka, gordana, Ajdžanović, Vladimir, "The effects of Vitex agnus castus L. essential oil on the pituitary cells in middle aged rats" in 23th International Congress Phytopharm; 2019 Jul 1-3; Saint-Petersburg, Russia (2019):40,
https://hdl.handle.net/21.15107/rcub_ibiss_5927 .

TY  - JOUR
AU  - Ristić, Nataša
AU  - Ajdžanović, Vladimir
AU  - Petrović-Kosanović, Dragana
AU  - Miler, Marko
AU  - Ušćebrka, Gordana
AU  - Milošević, Verica
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3168
AB  - Andropause, the culminating phase of male ageing, is characterized by deregulation of the hypothalamic-pituitarygonadal axis and low circulating free testosterone. The aim of this study was to investigate the immunohistomorphometric characteristics of the pituitary gonadotropic i.e. follicle-stimulating hormone (FSH) and the luteinizing hormone (LH) producing cells after testosterone application in a rat model of the andropause. Middle-aged Wistar rats were divided into orchidectomized (ORX; n=8) and testosterone treated orchidectomized (ORX+T; n=8) groups. Testosterone propionate (5 mg/kg b.m. /day) was administered for three weeks, while the ORX group received the vehicle alone. Immunohistochemically stained FSH and LH cells underwent morphometric and optical density-related analysis, while circulating concentrations of the sex steroids were measured by immunoassays. Serum concentrations of testosterone and estradiol were significantly (p<0.05) increased by 24 and 2.7 fold respectively, compared to the ORX group. The volume of FSH and LH cells was significantly (p<0.05) decreased by 51.3% and 56.6% respectively, in comparison with ORX rats. Relative volume density of FSH and LH cells was also significantly (p<0.05) decreased by 54.0% and 72.8% respectively, compared to the ORX group. Results related to the optical density of gonadotropic cells (reflecting their hormonal content) were in line with the morphometric findings i.e. this parameter of FSH and LH cells was significantly (p<0.05) decreased by 25.7% and 16.2% respectively, in comparison with ORX rats. Conclusion: In conclusion, applied testosterone increased the serum concentrations of sex steroids, as well as it decreased morphometric parameters and optical density of gonadotropic cells in ORX rats.
T2  - Macedonian Veterinary Review
T1  - Immunohistomorphometric changes of the pituitary gonadotropic cells after testosterone application in a rat model of the andropause
IS  - 1
VL  - 42
DO  - 10.2478/macvetrev-2018-0023
SP  - 5
EP  - 13
ER  - 

@article{
author = "Ristić, Nataša and Ajdžanović, Vladimir and Petrović-Kosanović, Dragana and Miler, Marko and Ušćebrka, Gordana and Milošević, Verica",
year = "2019",
abstract = "Andropause, the culminating phase of male ageing, is characterized by deregulation of the hypothalamic-pituitarygonadal axis and low circulating free testosterone. The aim of this study was to investigate the immunohistomorphometric characteristics of the pituitary gonadotropic i.e. follicle-stimulating hormone (FSH) and the luteinizing hormone (LH) producing cells after testosterone application in a rat model of the andropause. Middle-aged Wistar rats were divided into orchidectomized (ORX; n=8) and testosterone treated orchidectomized (ORX+T; n=8) groups. Testosterone propionate (5 mg/kg b.m. /day) was administered for three weeks, while the ORX group received the vehicle alone. Immunohistochemically stained FSH and LH cells underwent morphometric and optical density-related analysis, while circulating concentrations of the sex steroids were measured by immunoassays. Serum concentrations of testosterone and estradiol were significantly (p<0.05) increased by 24 and 2.7 fold respectively, compared to the ORX group. The volume of FSH and LH cells was significantly (p<0.05) decreased by 51.3% and 56.6% respectively, in comparison with ORX rats. Relative volume density of FSH and LH cells was also significantly (p<0.05) decreased by 54.0% and 72.8% respectively, compared to the ORX group. Results related to the optical density of gonadotropic cells (reflecting their hormonal content) were in line with the morphometric findings i.e. this parameter of FSH and LH cells was significantly (p<0.05) decreased by 25.7% and 16.2% respectively, in comparison with ORX rats. Conclusion: In conclusion, applied testosterone increased the serum concentrations of sex steroids, as well as it decreased morphometric parameters and optical density of gonadotropic cells in ORX rats.",
journal = "Macedonian Veterinary Review",
title = "Immunohistomorphometric changes of the pituitary gonadotropic cells after testosterone application in a rat model of the andropause",
number = "1",
volume = "42",
doi = "10.2478/macvetrev-2018-0023",
pages = "5-13"
}

Ristić, N., Ajdžanović, V., Petrović-Kosanović, D., Miler, M., Ušćebrka, G.,& Milošević, V.. (2019). Immunohistomorphometric changes of the pituitary gonadotropic cells after testosterone application in a rat model of the andropause. in Macedonian Veterinary Review, 42(1), 5-13.
https://doi.org/10.2478/macvetrev-2018-0023

Ristić N, Ajdžanović V, Petrović-Kosanović D, Miler M, Ušćebrka G, Milošević V. Immunohistomorphometric changes of the pituitary gonadotropic cells after testosterone application in a rat model of the andropause. in Macedonian Veterinary Review. 2019;42(1):5-13.
doi:10.2478/macvetrev-2018-0023 .

Ristić, Nataša, Ajdžanović, Vladimir, Petrović-Kosanović, Dragana, Miler, Marko, Ušćebrka, Gordana, Milošević, Verica, "Immunohistomorphometric changes of the pituitary gonadotropic cells after testosterone application in a rat model of the andropause" in Macedonian Veterinary Review, 42, no. 1 (2019):5-13,
https://doi.org/10.2478/macvetrev-2018-0023 . .

TY  - CONF
AU  - Šošić-Jurjević, Branka
AU  - Lütjohann, Dieter
AU  - Miljić, Dragana
AU  - Ćirić, Jasmina
AU  - Trifunović, Svetlana
AU  - Ajdžanović, Vladimir
AU  - Filipović, Branko
AU  - Köhrle, Josef
AU  - Ušćebrka, Gordana
AU  - Milošević, Verica
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6206
AB  - Obesity, a major public health problem, is associated with hypercholesterolemia and other metabolic disorders, which are potent risk factors for athero-thrombotic disease, a leading cause of mortality worldwide. Advances in both dietary and pharmacological interventions contribute significantly to prevention and treatment of modifiable risk factors. Consumption of purified soybean isoflavones was reported to reduce plasma and liver cholesterol levels. The precise mechanism has not been established, but it has been suggested that these effects occur through an increase in bile acid excretion. The initial and rate limiting step in the classical pathway of hepatic synthesis of bile acids from cholesterol is the enzymatic addition of a 7α hydroxyl group by cholesterol 7α-hydroxylase forming 7α-hydroxycholesterol. Thyroid hormones have been shown to up-regulate expression of this enzyme. We previously reported that subcutaneously administered genistein impaired thyroid functioning in middle-aged rats, but at the same time increased expression of T3-activated genes and increased deiodinase type 1 enzyme activity in the liver. In this study we aimed to further determine effects of genistein on concentrations of thyroid hormones, total cholesterol and 7α-Hydroxycholesterol in the liver. Thirteen-month-old male Wistar rats were injected subcutaneously with 35 mg/kg of genistein, while controls received vehicle alone daily during 4 weeks. For determination of iodothyronines liquid chromatography-mass spectrometry was applied, while total cholesterol and 7α-hydroxycholesterol were determined by gas chromatography/mass spectrometry. Our study results clearly demonstrate that genistein increased (P<0.05) concentration of T4 and T3 in the liver of middle-aged male rats. In line with this, concentration of 7α-hydroxycholesterol also increased (P<0.05), but the total cholesterol levels remained unchanged. In conclusion, genistein increased hepatic availability of thyroid hormones, in accordance with detected increase in 7α-hydroxycholesterol level in the liver. The obtained data brought new light on the mechanisms involved in biological processes in the liver induced by genistein.
PB  - Bioscientifica
C3  - 20th European Congress of Endocrinology; 2018 May 19-22; Barcelona, Spain
T1  - Genistein Increase Thyroid Hormone and 7 alpha-hydroxycholesterol concentrations in the liver of middle-aged male rats
DO  - 10.1530/endoabs.56.gp246
SP  - GP246
ER  - 

@conference{
author = "Šošić-Jurjević, Branka and Lütjohann, Dieter and Miljić, Dragana and Ćirić, Jasmina and Trifunović, Svetlana and Ajdžanović, Vladimir and Filipović, Branko and Köhrle, Josef and Ušćebrka, Gordana and Milošević, Verica",
year = "2018",
abstract = "Obesity, a major public health problem, is associated with hypercholesterolemia and other metabolic disorders, which are potent risk factors for athero-thrombotic disease, a leading cause of mortality worldwide. Advances in both dietary and pharmacological interventions contribute significantly to prevention and treatment of modifiable risk factors. Consumption of purified soybean isoflavones was reported to reduce plasma and liver cholesterol levels. The precise mechanism has not been established, but it has been suggested that these effects occur through an increase in bile acid excretion. The initial and rate limiting step in the classical pathway of hepatic synthesis of bile acids from cholesterol is the enzymatic addition of a 7α hydroxyl group by cholesterol 7α-hydroxylase forming 7α-hydroxycholesterol. Thyroid hormones have been shown to up-regulate expression of this enzyme. We previously reported that subcutaneously administered genistein impaired thyroid functioning in middle-aged rats, but at the same time increased expression of T3-activated genes and increased deiodinase type 1 enzyme activity in the liver. In this study we aimed to further determine effects of genistein on concentrations of thyroid hormones, total cholesterol and 7α-Hydroxycholesterol in the liver. Thirteen-month-old male Wistar rats were injected subcutaneously with 35 mg/kg of genistein, while controls received vehicle alone daily during 4 weeks. For determination of iodothyronines liquid chromatography-mass spectrometry was applied, while total cholesterol and 7α-hydroxycholesterol were determined by gas chromatography/mass spectrometry. Our study results clearly demonstrate that genistein increased (P<0.05) concentration of T4 and T3 in the liver of middle-aged male rats. In line with this, concentration of 7α-hydroxycholesterol also increased (P<0.05), but the total cholesterol levels remained unchanged. In conclusion, genistein increased hepatic availability of thyroid hormones, in accordance with detected increase in 7α-hydroxycholesterol level in the liver. The obtained data brought new light on the mechanisms involved in biological processes in the liver induced by genistein.",
publisher = "Bioscientifica",
journal = "20th European Congress of Endocrinology; 2018 May 19-22; Barcelona, Spain",
title = "Genistein Increase Thyroid Hormone and 7 alpha-hydroxycholesterol concentrations in the liver of middle-aged male rats",
doi = "10.1530/endoabs.56.gp246",
pages = "GP246"
}

Šošić-Jurjević, B., Lütjohann, D., Miljić, D., Ćirić, J., Trifunović, S., Ajdžanović, V., Filipović, B., Köhrle, J., Ušćebrka, G.,& Milošević, V.. (2018). Genistein Increase Thyroid Hormone and 7 alpha-hydroxycholesterol concentrations in the liver of middle-aged male rats. in 20th European Congress of Endocrinology; 2018 May 19-22; Barcelona, Spain
Bioscientifica., GP246.
https://doi.org/10.1530/endoabs.56.gp246

Šošić-Jurjević B, Lütjohann D, Miljić D, Ćirić J, Trifunović S, Ajdžanović V, Filipović B, Köhrle J, Ušćebrka G, Milošević V. Genistein Increase Thyroid Hormone and 7 alpha-hydroxycholesterol concentrations in the liver of middle-aged male rats. in 20th European Congress of Endocrinology; 2018 May 19-22; Barcelona, Spain. 2018;:GP246.
doi:10.1530/endoabs.56.gp246 .

Šošić-Jurjević, Branka, Lütjohann, Dieter, Miljić, Dragana, Ćirić, Jasmina, Trifunović, Svetlana, Ajdžanović, Vladimir, Filipović, Branko, Köhrle, Josef, Ušćebrka, Gordana, Milošević, Verica, "Genistein Increase Thyroid Hormone and 7 alpha-hydroxycholesterol concentrations in the liver of middle-aged male rats" in 20th European Congress of Endocrinology; 2018 May 19-22; Barcelona, Spain (2018):GP246,
https://doi.org/10.1530/endoabs.56.gp246 . .

TY  - JOUR
AU  - Ušćebrka, Gordana
AU  - Žikić, Dragan
AU  - Stojanović, Slobodan
AU  - Miler, Marko
AU  - Jarić, Ivana
AU  - Ajdžanović, Vladimir
AU  - Milošević, Verica
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6038
AB  - One of the goals ofrnodern poultry production is improving the quality and quantity of
broiler meat. Recent research is directed to find the relation between incubation factors and
muscle development, which can affect on meat characteristics. The aim of this study was
to examine the influence of increased incubation temperature on development of breast
muscles of broilers in days after hatching. Eight hundred broiler eggs (Ross 308) were
divided in four equal groups (200 per each group) and placed in four incubators. Group
A represents control group with incubation temperature 378°C. In groups B, C, D during
the 16‘“, 17‘“ and 18th day of incubation temperature was increased to 38.5°C, 395°C and
41°C reSpectively, for 3 hours each day. On the 7‘1‘, as well as on the 14‘“ day after hatching,
10 chickens from each group were sacrificed. Samples of breast muscle (M pectoralis
superficialis) were taken for histological examination of diameter of muscle cells, volume
density of connective tissue in the muscle and nucleo-cytoplasmic ratio of muscle cells.
Measurements showed that diameter of breast muscle cells on 7L11 day after hatching in
treated groups was decreased in average by 6.36pm compared to control group, while on
 14m day after hatching in group in which incubation temperature was increased to 41°C,
it was decreased in average by 5.84pm compared to other groups. In all groups, on 14‘h
day after hatching, diameter of breast muscle cells was increased in average by 6.71pm,
while nucleo—cytoplasmic ratio of breast muscle cells was decreased in average by 0.035,
compared to 7”h day after hatching. Lower diameter of breast muscle cells in treated groups
on 7‘“ day after hatching can be explained by higher proliferation of myoblasts due to
applied treatments. During the proliferation, growth of cells is reduced, which is reflected
on lower diameter of muscle cells on 7"1 day after hatching. It can be assumed that increased
temperature during incubation affect on increased growth of muscle cells in postnatal
period of development, which is in agreement with approximately equal diameters on 14*
day after hatching between control group and groups in which incubation temperature was
increased to 385°C and 395°C. It seems that increased temperature on 41"C had negative
effect on growth and development of muscle cells, which results in decreased diameter of
breast muscle cells on 14‘h day afier hatching in group which incubation temperature was
increased to 41°C.
PB  - Skopje: Faculty of Veterinary Medicine
T2  - Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia
T1  - Influence of Increased Incubation Temperature on Breast Muscle Development of Broilers After Hatching
SP  - 146
EP  - 146
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6038
ER  - 

@article{
author = "Ušćebrka, Gordana and Žikić, Dragan and Stojanović, Slobodan and Miler, Marko and Jarić, Ivana and Ajdžanović, Vladimir and Milošević, Verica",
year = "2018",
abstract = "One of the goals ofrnodern poultry production is improving the quality and quantity of
broiler meat. Recent research is directed to find the relation between incubation factors and
muscle development, which can affect on meat characteristics. The aim of this study was
to examine the influence of increased incubation temperature on development of breast
muscles of broilers in days after hatching. Eight hundred broiler eggs (Ross 308) were
divided in four equal groups (200 per each group) and placed in four incubators. Group
A represents control group with incubation temperature 378°C. In groups B, C, D during
the 16‘“, 17‘“ and 18th day of incubation temperature was increased to 38.5°C, 395°C and
41°C reSpectively, for 3 hours each day. On the 7‘1‘, as well as on the 14‘“ day after hatching,
10 chickens from each group were sacrificed. Samples of breast muscle (M pectoralis
superficialis) were taken for histological examination of diameter of muscle cells, volume
density of connective tissue in the muscle and nucleo-cytoplasmic ratio of muscle cells.
Measurements showed that diameter of breast muscle cells on 7L11 day after hatching in
treated groups was decreased in average by 6.36pm compared to control group, while on
 14m day after hatching in group in which incubation temperature was increased to 41°C,
it was decreased in average by 5.84pm compared to other groups. In all groups, on 14‘h
day after hatching, diameter of breast muscle cells was increased in average by 6.71pm,
while nucleo—cytoplasmic ratio of breast muscle cells was decreased in average by 0.035,
compared to 7”h day after hatching. Lower diameter of breast muscle cells in treated groups
on 7‘“ day after hatching can be explained by higher proliferation of myoblasts due to
applied treatments. During the proliferation, growth of cells is reduced, which is reflected
on lower diameter of muscle cells on 7"1 day after hatching. It can be assumed that increased
temperature during incubation affect on increased growth of muscle cells in postnatal
period of development, which is in agreement with approximately equal diameters on 14*
day after hatching between control group and groups in which incubation temperature was
increased to 385°C and 395°C. It seems that increased temperature on 41"C had negative
effect on growth and development of muscle cells, which results in decreased diameter of
breast muscle cells on 14‘h day afier hatching in group which incubation temperature was
increased to 41°C.",
publisher = "Skopje: Faculty of Veterinary Medicine",
journal = "Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia",
title = "Influence of Increased Incubation Temperature on Breast Muscle Development of Broilers After Hatching",
pages = "146-146",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6038"
}

Ušćebrka, G., Žikić, D., Stojanović, S., Miler, M., Jarić, I., Ajdžanović, V.,& Milošević, V.. (2018). Influence of Increased Incubation Temperature on Breast Muscle Development of Broilers After Hatching. in Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia
Skopje: Faculty of Veterinary Medicine., 146-146.
https://hdl.handle.net/21.15107/rcub_ibiss_6038

Ušćebrka G, Žikić D, Stojanović S, Miler M, Jarić I, Ajdžanović V, Milošević V. Influence of Increased Incubation Temperature on Breast Muscle Development of Broilers After Hatching. in Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia. 2018;:146-146.
https://hdl.handle.net/21.15107/rcub_ibiss_6038 .

Ušćebrka, Gordana, Žikić, Dragan, Stojanović, Slobodan, Miler, Marko, Jarić, Ivana, Ajdžanović, Vladimir, Milošević, Verica, "Influence of Increased Incubation Temperature on Breast Muscle Development of Broilers After Hatching" in Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia (2018):146-146,
https://hdl.handle.net/21.15107/rcub_ibiss_6038 .

TY  - CONF
AU  - Jarić, Ivana
AU  - Miler, Marko
AU  - Ajdžanović, Vladimir
AU  - Živanović, Jasmina
AU  - Pendoski, Laze
AU  - Žikić, Dragan
AU  - Ušćebrka, Gordana
AU  - Milošević, Verica
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6037
AB  - Diosgenin is bioactive, steroidal saponin from Dioscorea viliosa (Wild Yam) and appears to be novel promising substance for the treatment of menopausal symptoms. In addition, diosgenin may manipulate the endogenous steroid hormone levels and may affect the numerous oxidative stress pathways/molecules. Since decline in the steroid hormone levels as well as increase in oxidative stress in the ovaries play an integral part of the menopause, this study aimed to investigate the effects of diosgenin on production of estradiol and progesterone, as well as to investigated the effects of diosgenin on the expression of genes encoding the antioxidant enzymes (Cu, Zn-superoxide dismutase (CuZnSOD), Mn-superoxide dismutase (MnSOD), glutathione peroxidase (GPX), and catalase (CAD) in ovary of acyclic-middle aged female rats. Middle-aged (l3-months-old) acyclic female Wistar rats in constant diestrus stage, which represent tractable model to human menopause, were divided into control and diosgenin treated groups.Diosgenin (100 mgfl<g b.w.lday) was orally applied for 4 weeks, while C group received the vehicle alone. In our experimental model, diosgenin administration to acyclic middle-aged rats significantly increased estradiol and progesterone serum concentrations when compared to the corresponding control. Gene expression analysis revealed that diosgenin significantly down-regulate all examined enzymes involved in antioxidative defense. The down-regulation were especially pronounced in CuZnSOD and CAT, indicating that the diosgenin may decrease the pool of reactive oxygen species in the ovary directly due and may act as free radical scavengers. Therefore, our results indicate beneficial potential of diosgenin in the middle-aged rat ovary.
PB  - Skopje: Faculty of Veterinary Medicine
C3  - Book of abstracts: join meeting: 5th International Vet-Istanbul group congress and 8th International scientific meeting days of veterinary medicine; 2018, Sep 23-27; Ohrid, Macedonia
T1  - Diosgenin Affects Steroid Hormone Production and Gene Expression of Antioxidative Enzymes in the Ovary of Middle-aged Rats
SP  - 147
EP  - 147
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6037
ER  - 

@conference{
author = "Jarić, Ivana and Miler, Marko and Ajdžanović, Vladimir and Živanović, Jasmina and Pendoski, Laze and Žikić, Dragan and Ušćebrka, Gordana and Milošević, Verica",
year = "2018",
abstract = "Diosgenin is bioactive, steroidal saponin from Dioscorea viliosa (Wild Yam) and appears to be novel promising substance for the treatment of menopausal symptoms. In addition, diosgenin may manipulate the endogenous steroid hormone levels and may affect the numerous oxidative stress pathways/molecules. Since decline in the steroid hormone levels as well as increase in oxidative stress in the ovaries play an integral part of the menopause, this study aimed to investigate the effects of diosgenin on production of estradiol and progesterone, as well as to investigated the effects of diosgenin on the expression of genes encoding the antioxidant enzymes (Cu, Zn-superoxide dismutase (CuZnSOD), Mn-superoxide dismutase (MnSOD), glutathione peroxidase (GPX), and catalase (CAD) in ovary of acyclic-middle aged female rats. Middle-aged (l3-months-old) acyclic female Wistar rats in constant diestrus stage, which represent tractable model to human menopause, were divided into control and diosgenin treated groups.Diosgenin (100 mgfl<g b.w.lday) was orally applied for 4 weeks, while C group received the vehicle alone. In our experimental model, diosgenin administration to acyclic middle-aged rats significantly increased estradiol and progesterone serum concentrations when compared to the corresponding control. Gene expression analysis revealed that diosgenin significantly down-regulate all examined enzymes involved in antioxidative defense. The down-regulation were especially pronounced in CuZnSOD and CAT, indicating that the diosgenin may decrease the pool of reactive oxygen species in the ovary directly due and may act as free radical scavengers. Therefore, our results indicate beneficial potential of diosgenin in the middle-aged rat ovary.",
publisher = "Skopje: Faculty of Veterinary Medicine",
journal = "Book of abstracts: join meeting: 5th International Vet-Istanbul group congress and 8th International scientific meeting days of veterinary medicine; 2018, Sep 23-27; Ohrid, Macedonia",
title = "Diosgenin Affects Steroid Hormone Production and Gene Expression of Antioxidative Enzymes in the Ovary of Middle-aged Rats",
pages = "147-147",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6037"
}

Jarić, I., Miler, M., Ajdžanović, V., Živanović, J., Pendoski, L., Žikić, D., Ušćebrka, G.,& Milošević, V.. (2018). Diosgenin Affects Steroid Hormone Production and Gene Expression of Antioxidative Enzymes in the Ovary of Middle-aged Rats. in Book of abstracts: join meeting: 5th International Vet-Istanbul group congress and 8th International scientific meeting days of veterinary medicine; 2018, Sep 23-27; Ohrid, Macedonia
Skopje: Faculty of Veterinary Medicine., 147-147.
https://hdl.handle.net/21.15107/rcub_ibiss_6037

Jarić I, Miler M, Ajdžanović V, Živanović J, Pendoski L, Žikić D, Ušćebrka G, Milošević V. Diosgenin Affects Steroid Hormone Production and Gene Expression of Antioxidative Enzymes in the Ovary of Middle-aged Rats. in Book of abstracts: join meeting: 5th International Vet-Istanbul group congress and 8th International scientific meeting days of veterinary medicine; 2018, Sep 23-27; Ohrid, Macedonia. 2018;:147-147.
https://hdl.handle.net/21.15107/rcub_ibiss_6037 .

Jarić, Ivana, Miler, Marko, Ajdžanović, Vladimir, Živanović, Jasmina, Pendoski, Laze, Žikić, Dragan, Ušćebrka, Gordana, Milošević, Verica, "Diosgenin Affects Steroid Hormone Production and Gene Expression of Antioxidative Enzymes in the Ovary of Middle-aged Rats" in Book of abstracts: join meeting: 5th International Vet-Istanbul group congress and 8th International scientific meeting days of veterinary medicine; 2018, Sep 23-27; Ohrid, Macedonia (2018):147-147,
https://hdl.handle.net/21.15107/rcub_ibiss_6037 .

TY  - CONF
AU  - Popovska-Perčinić, Florina
AU  - Milošević, Verica
AU  - Pendovski, Lazo
AU  - Potrebić, Milica
AU  - Ušćebrka, Gordana
AU  - Sretenović, Jasmina
AU  - Jarić, Ivana
AU  - Ajdžanović, Vladimir
PY  - 2018
UR  - http://www.enea2018.com/program.html
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5925
PB  - European NeuroEndocrine Association
C3  - 18th Congress of the European NeuroEndocrine Association; 2018 Oct 17-20; Wrocław, Poland
T1  - The effect of long-term exposure to moderately high ambient temperature on rat pituitary corticotropes: immunohistomorphometric and hormonal study
SP  - 8
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5925
ER  - 

@conference{
author = "Popovska-Perčinić, Florina and Milošević, Verica and Pendovski, Lazo and Potrebić, Milica and Ušćebrka, Gordana and Sretenović, Jasmina and Jarić, Ivana and Ajdžanović, Vladimir",
year = "2018",
publisher = "European NeuroEndocrine Association",
journal = "18th Congress of the European NeuroEndocrine Association; 2018 Oct 17-20; Wrocław, Poland",
title = "The effect of long-term exposure to moderately high ambient temperature on rat pituitary corticotropes: immunohistomorphometric and hormonal study",
pages = "8",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5925"
}

Popovska-Perčinić, F., Milošević, V., Pendovski, L., Potrebić, M., Ušćebrka, G., Sretenović, J., Jarić, I.,& Ajdžanović, V.. (2018). The effect of long-term exposure to moderately high ambient temperature on rat pituitary corticotropes: immunohistomorphometric and hormonal study. in 18th Congress of the European NeuroEndocrine Association; 2018 Oct 17-20; Wrocław, Poland
European NeuroEndocrine Association., 8.
https://hdl.handle.net/21.15107/rcub_ibiss_5925

Popovska-Perčinić F, Milošević V, Pendovski L, Potrebić M, Ušćebrka G, Sretenović J, Jarić I, Ajdžanović V. The effect of long-term exposure to moderately high ambient temperature on rat pituitary corticotropes: immunohistomorphometric and hormonal study. in 18th Congress of the European NeuroEndocrine Association; 2018 Oct 17-20; Wrocław, Poland. 2018;:8.
https://hdl.handle.net/21.15107/rcub_ibiss_5925 .

Popovska-Perčinić, Florina, Milošević, Verica, Pendovski, Lazo, Potrebić, Milica, Ušćebrka, Gordana, Sretenović, Jasmina, Jarić, Ivana, Ajdžanović, Vladimir, "The effect of long-term exposure to moderately high ambient temperature on rat pituitary corticotropes: immunohistomorphometric and hormonal study" in 18th Congress of the European NeuroEndocrine Association; 2018 Oct 17-20; Wrocław, Poland (2018):8,
https://hdl.handle.net/21.15107/rcub_ibiss_5925 .

TY  - CONF
AU  - Miler, Marko
AU  - Ajdžanović, Vladimir
AU  - Ristić, Nataša
AU  - Filipović, Branko
AU  - Ušćebrka, Gordana
AU  - Miljić, Dragana
AU  - Milošević, Verica
AU  - Šošić-Jurjević, Branka 
PY  - 2017
UR  - https://www.endocrine-abstracts.org/ea/0049/ECE2017AbstractBook.pdf
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3505
AB  - Aging is a complex process characterized by a progressive decline in cellular
function, alterations of the endocrine system and the increased incidence of
thyroid diseases. Citrus flavanones naringenin (NAR) and hesperetin (HES) may
contribute to maintenance of health at old age however they may also affect
thyroid hormone economy. The aim of this study was to test weather NAR or HES
administration alter thyroid functioning. NAR or HES were administrated orally
(15 mg/kg) to male 24-month-old Wistar rats during 4 weeks. Control groups
received vehicle, sunflower oil. Immunohistochemical staining of thyroglobulin
(Tg), thyroxine (T4) bound to Tg (T4-Tg) and sodium iodide sysmporter (NIS)
were performed. Immunopositivity of Tg or T4-Tg in thyroid follicles were
measured as intensity of optical density (OD) or relative intensity of fluorescence
(RIF). Thyroid stimulating hormone (TSH) and total T4 concentrations were
measured in serum. After citrus flavanones treatments, thyroids showed Tg and
T4-Tg immunopositivity mainly in the colloid of the follicles. The obtained
results showed increased (P ! 0.05) intensity of OD for Tg and T4-Tg RIF after
both flavanones treatments, while NIS expression did not change. Serum TSH
increased (P ! 0.05) only after NAR, although T4 remained unchanged after both
treatments. In conclusion, citrus flavanones induced stronger expression of
thyroid-specific proteins in gland of old-aged males. However, only NAR
elevated serum TSH, being more potent than HES. This alteration is in line with
preserved capacity of the thyroid to compensate the mild interference of
flavanones treatments with thyroid hormone production in old-aged males.
PB  - BioScientifica
C3  - 19th European Congress of Endocrinology; 2017 May 20-23; Lisbon, Portugal (Endocrine Abstracts; Vol. 49)
T1  - Citrus flavanones naringenin and hesperetin induced mild functional alteration in thyroids of old-aged Wistar rats
DO  - 10.1530/endoabs.49.GP202
SP  - GP202
ER  - 

@conference{
author = "Miler, Marko and Ajdžanović, Vladimir and Ristić, Nataša and Filipović, Branko and Ušćebrka, Gordana and Miljić, Dragana and Milošević, Verica and Šošić-Jurjević, Branka ",
year = "2017",
abstract = "Aging is a complex process characterized by a progressive decline in cellular
function, alterations of the endocrine system and the increased incidence of
thyroid diseases. Citrus flavanones naringenin (NAR) and hesperetin (HES) may
contribute to maintenance of health at old age however they may also affect
thyroid hormone economy. The aim of this study was to test weather NAR or HES
administration alter thyroid functioning. NAR or HES were administrated orally
(15 mg/kg) to male 24-month-old Wistar rats during 4 weeks. Control groups
received vehicle, sunflower oil. Immunohistochemical staining of thyroglobulin
(Tg), thyroxine (T4) bound to Tg (T4-Tg) and sodium iodide sysmporter (NIS)
were performed. Immunopositivity of Tg or T4-Tg in thyroid follicles were
measured as intensity of optical density (OD) or relative intensity of fluorescence
(RIF). Thyroid stimulating hormone (TSH) and total T4 concentrations were
measured in serum. After citrus flavanones treatments, thyroids showed Tg and
T4-Tg immunopositivity mainly in the colloid of the follicles. The obtained
results showed increased (P ! 0.05) intensity of OD for Tg and T4-Tg RIF after
both flavanones treatments, while NIS expression did not change. Serum TSH
increased (P ! 0.05) only after NAR, although T4 remained unchanged after both
treatments. In conclusion, citrus flavanones induced stronger expression of
thyroid-specific proteins in gland of old-aged males. However, only NAR
elevated serum TSH, being more potent than HES. This alteration is in line with
preserved capacity of the thyroid to compensate the mild interference of
flavanones treatments with thyroid hormone production in old-aged males.",
publisher = "BioScientifica",
journal = "19th European Congress of Endocrinology; 2017 May 20-23; Lisbon, Portugal (Endocrine Abstracts; Vol. 49)",
title = "Citrus flavanones naringenin and hesperetin induced mild functional alteration in thyroids of old-aged Wistar rats",
doi = "10.1530/endoabs.49.GP202",
pages = "GP202"
}

Miler, M., Ajdžanović, V., Ristić, N., Filipović, B., Ušćebrka, G., Miljić, D., Milošević, V.,& Šošić-Jurjević, B.. (2017). Citrus flavanones naringenin and hesperetin induced mild functional alteration in thyroids of old-aged Wistar rats. in 19th European Congress of Endocrinology; 2017 May 20-23; Lisbon, Portugal (Endocrine Abstracts; Vol. 49)
BioScientifica., GP202.
https://doi.org/10.1530/endoabs.49.GP202

Miler M, Ajdžanović V, Ristić N, Filipović B, Ušćebrka G, Miljić D, Milošević V, Šošić-Jurjević B. Citrus flavanones naringenin and hesperetin induced mild functional alteration in thyroids of old-aged Wistar rats. in 19th European Congress of Endocrinology; 2017 May 20-23; Lisbon, Portugal (Endocrine Abstracts; Vol. 49). 2017;:GP202.
doi:10.1530/endoabs.49.GP202 .

Miler, Marko, Ajdžanović, Vladimir, Ristić, Nataša, Filipović, Branko, Ušćebrka, Gordana, Miljić, Dragana, Milošević, Verica, Šošić-Jurjević, Branka , "Citrus flavanones naringenin and hesperetin induced mild functional alteration in thyroids of old-aged Wistar rats" in 19th European Congress of Endocrinology; 2017 May 20-23; Lisbon, Portugal (Endocrine Abstracts; Vol. 49) (2017):GP202,
https://doi.org/10.1530/endoabs.49.GP202 . .

TY  - CONF
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Živanović, Jasmina
AU  - Manojlović-Stojanoski, Milica
AU  - Nestorović, Nataša
AU  - Trifunović, Svetlana
AU  - Jarić, Ivana
AU  - Ušćebrka, Gordana
AU  - Milošević, Verica
PY  - 2017
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3432
AB  - Calcitonin (CT) is a hypocalcemic hormone produced by thyroid C-cells that acts as an bone antiresorptive agent. The other endocrine cell population in thyroid, called follicular cells produce thyroid hormones (TH) which also affect bone turnover. In this study, we evaluated the effects of salmon CT administration on structure and function of both CT and TH producing thyroid cells as well as trabecular bone microarchitecture in orchidectomized (Orx) middle-aged rats. Fifteen-month-old male Wistar rats were either Orx or sham-operated (SO). One group of Orx animals were injected subcutaneously with synthetic salmon CT (Orx + CT; 100 IU/kg b.w.) every second day for 6 weeks. The rats from SO and second Orx group received the same volume of vehicle alone by the same schedule. The peroxidase–antiperoxidase method was applied for localization of CT in C-cells. CT-immunopositive thyroid C-cells, thyroid follicular epithelium, interstitium and colloid were evaluated morphometrically. An ImageJ public domain image processing program was used to measure bone histomorphometric parameters of the proximal tibial specimens. Blood serum samples were analyzed for CT, osteocalcin (OC) and thyroxine (T4), and urine samples fot calcium (Ca2+) concentration. We found a significant decrease in the Vc and Vv of thyroid C-cells after CT treatment compared to both SO and Orx. The Vv of the colloid was higher, while VV of the follicular epithelium was lower after CT treatment compared to Orx. Analysis of trabecular microarchitecture showed that salmon CT administration significantly increases of cancellous bone area (B.Ar), trabecular thickness (Tb.Th), and trabecular number (Tb.N) whereas trabecular separation (Tb.Sp) was significantly decreased. CT treatment markedly elevated serum CT, but serum OC, T4 and urinary Ca2+ concentrations were lower than in the Orx group. These findings indicate that administration of salmon CT inhibited calcitonin-producing thyroid C-cells and changes the structure of the thyroid gland indicating hypoactivity.
PB  - BioScientifica
C3  - 19th European Congress of Endocrinology; Lisbon, Portugal; 2017 May 20-23. (Endocrine Abstracts; Vol. 49)
T1  - Salmon calcitonin affects thyroid endocrine cells and trabecular bone in a rat model of male osteoporosis
DO  - 10.1530/endoabs.49.EP212
SP  - EP212
ER  - 

@conference{
author = "Filipović, Branko and Šošić-Jurjević, Branka  and Živanović, Jasmina and Manojlović-Stojanoski, Milica and Nestorović, Nataša and Trifunović, Svetlana and Jarić, Ivana and Ušćebrka, Gordana and Milošević, Verica",
year = "2017",
abstract = "Calcitonin (CT) is a hypocalcemic hormone produced by thyroid C-cells that acts as an bone antiresorptive agent. The other endocrine cell population in thyroid, called follicular cells produce thyroid hormones (TH) which also affect bone turnover. In this study, we evaluated the effects of salmon CT administration on structure and function of both CT and TH producing thyroid cells as well as trabecular bone microarchitecture in orchidectomized (Orx) middle-aged rats. Fifteen-month-old male Wistar rats were either Orx or sham-operated (SO). One group of Orx animals were injected subcutaneously with synthetic salmon CT (Orx + CT; 100 IU/kg b.w.) every second day for 6 weeks. The rats from SO and second Orx group received the same volume of vehicle alone by the same schedule. The peroxidase–antiperoxidase method was applied for localization of CT in C-cells. CT-immunopositive thyroid C-cells, thyroid follicular epithelium, interstitium and colloid were evaluated morphometrically. An ImageJ public domain image processing program was used to measure bone histomorphometric parameters of the proximal tibial specimens. Blood serum samples were analyzed for CT, osteocalcin (OC) and thyroxine (T4), and urine samples fot calcium (Ca2+) concentration. We found a significant decrease in the Vc and Vv of thyroid C-cells after CT treatment compared to both SO and Orx. The Vv of the colloid was higher, while VV of the follicular epithelium was lower after CT treatment compared to Orx. Analysis of trabecular microarchitecture showed that salmon CT administration significantly increases of cancellous bone area (B.Ar), trabecular thickness (Tb.Th), and trabecular number (Tb.N) whereas trabecular separation (Tb.Sp) was significantly decreased. CT treatment markedly elevated serum CT, but serum OC, T4 and urinary Ca2+ concentrations were lower than in the Orx group. These findings indicate that administration of salmon CT inhibited calcitonin-producing thyroid C-cells and changes the structure of the thyroid gland indicating hypoactivity.",
publisher = "BioScientifica",
journal = "19th European Congress of Endocrinology; Lisbon, Portugal; 2017 May 20-23. (Endocrine Abstracts; Vol. 49)",
title = "Salmon calcitonin affects thyroid endocrine cells and trabecular bone in a rat model of male osteoporosis",
doi = "10.1530/endoabs.49.EP212",
pages = "EP212"
}

Filipović, B., Šošić-Jurjević, B., Živanović, J., Manojlović-Stojanoski, M., Nestorović, N., Trifunović, S., Jarić, I., Ušćebrka, G.,& Milošević, V.. (2017). Salmon calcitonin affects thyroid endocrine cells and trabecular bone in a rat model of male osteoporosis. in 19th European Congress of Endocrinology; Lisbon, Portugal; 2017 May 20-23. (Endocrine Abstracts; Vol. 49)
BioScientifica., EP212.
https://doi.org/10.1530/endoabs.49.EP212

Filipović B, Šošić-Jurjević B, Živanović J, Manojlović-Stojanoski M, Nestorović N, Trifunović S, Jarić I, Ušćebrka G, Milošević V. Salmon calcitonin affects thyroid endocrine cells and trabecular bone in a rat model of male osteoporosis. in 19th European Congress of Endocrinology; Lisbon, Portugal; 2017 May 20-23. (Endocrine Abstracts; Vol. 49). 2017;:EP212.
doi:10.1530/endoabs.49.EP212 .

Filipović, Branko, Šošić-Jurjević, Branka , Živanović, Jasmina, Manojlović-Stojanoski, Milica, Nestorović, Nataša, Trifunović, Svetlana, Jarić, Ivana, Ušćebrka, Gordana, Milošević, Verica, "Salmon calcitonin affects thyroid endocrine cells and trabecular bone in a rat model of male osteoporosis" in 19th European Congress of Endocrinology; Lisbon, Portugal; 2017 May 20-23. (Endocrine Abstracts; Vol. 49) (2017):EP212,
https://doi.org/10.1530/endoabs.49.EP212 . .

TY  - JOUR
AU  - Ristić, Nataša
AU  - Ajdžanović, Vladimir
AU  - Manojlović-Stojanoski, Milica
AU  - Maliković, Jovana
AU  - Ušćebrka, Gordana
AU  - Marković, Zorica
AU  - Milošević, Verica
PY  - 2017
UR  - http://celljournal.org/journal/article/abstract/4334
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2846
AB  - Objective: Estrogen replacement therapy remains current as a therapeutic approach to treat menopausal symptoms and may significantly affect hormone-producing cells in the female pituitaries. The aim of this study was to examine the histological parameters of pituitary mammotrophs and prolactin secretion after chronic estradiol treatment in ovariectomized adult female rats, reflecting premature menopause. Materials and Methods: In this experimental study, adult female Wistar rats were divided into non-ovariectomized (C), ovariectomized (OVX) and estradiol-treated ovariectomized (OVX+E) groups. Estradiol dipropionate [0.625 mg/kg body mass per day] was administered for four weeks, while the C and OVX groups received vehicle alone. Mammotrophs were identified by the peroxidase-antiperoxidase (PAP) immunohistochemical procedure, while prolactin concentrations were measured by the non-isotopic two-step assay (Delfia) method. Comparison of the differences between groups was performed using one-way analysis of variance (ANOVA) and Tukay (honest significant difference) HSD test. Results: Ovariectomy caused significant (P < 0.05) decreases in mammotroph optical density (OD), volume density (V V ) and number per mm2 by 29, 27 and 34%, respectively, in comparison with the C females. In the OVX+E group, significant (P < 0.05) increases in OD, cell volume, V V and number of mammotrophs per mm2 by 181, 15%, 5.8-fold and 5.2-fold, respectively, were observed when compared to OVX animals. The serum prolactin concentration in OVX females was significantly (P < 0.05) decreased by 14% in comparison to the C group, while in OVX+E females, prolactin levels were significantly (P < 0.05) increased by 53% compared to the OVX controls. Conclusion: Estradiol supplementation in ovariectomized females is followed by stimulatory histological and secretory changes of the mammotrophs. These results could serve as indicators of possible prolactinome development upon estradiol application in premature menopausal subjects.
T2  - Cell Journal
T1  - Effects of estradiol on histological parameters and secretory ability of pituitary mammotrophs in ovariectomized female rats
IS  - 3
VL  - 19
DO  - 10.22074/cellj.2017.4334
SP  - 461
EP  - 468
ER  - 

@article{
author = "Ristić, Nataša and Ajdžanović, Vladimir and Manojlović-Stojanoski, Milica and Maliković, Jovana and Ušćebrka, Gordana and Marković, Zorica and Milošević, Verica",
year = "2017",
abstract = "Objective: Estrogen replacement therapy remains current as a therapeutic approach to treat menopausal symptoms and may significantly affect hormone-producing cells in the female pituitaries. The aim of this study was to examine the histological parameters of pituitary mammotrophs and prolactin secretion after chronic estradiol treatment in ovariectomized adult female rats, reflecting premature menopause. Materials and Methods: In this experimental study, adult female Wistar rats were divided into non-ovariectomized (C), ovariectomized (OVX) and estradiol-treated ovariectomized (OVX+E) groups. Estradiol dipropionate [0.625 mg/kg body mass per day] was administered for four weeks, while the C and OVX groups received vehicle alone. Mammotrophs were identified by the peroxidase-antiperoxidase (PAP) immunohistochemical procedure, while prolactin concentrations were measured by the non-isotopic two-step assay (Delfia) method. Comparison of the differences between groups was performed using one-way analysis of variance (ANOVA) and Tukay (honest significant difference) HSD test. Results: Ovariectomy caused significant (P < 0.05) decreases in mammotroph optical density (OD), volume density (V V ) and number per mm2 by 29, 27 and 34%, respectively, in comparison with the C females. In the OVX+E group, significant (P < 0.05) increases in OD, cell volume, V V and number of mammotrophs per mm2 by 181, 15%, 5.8-fold and 5.2-fold, respectively, were observed when compared to OVX animals. The serum prolactin concentration in OVX females was significantly (P < 0.05) decreased by 14% in comparison to the C group, while in OVX+E females, prolactin levels were significantly (P < 0.05) increased by 53% compared to the OVX controls. Conclusion: Estradiol supplementation in ovariectomized females is followed by stimulatory histological and secretory changes of the mammotrophs. These results could serve as indicators of possible prolactinome development upon estradiol application in premature menopausal subjects.",
journal = "Cell Journal",
title = "Effects of estradiol on histological parameters and secretory ability of pituitary mammotrophs in ovariectomized female rats",
number = "3",
volume = "19",
doi = "10.22074/cellj.2017.4334",
pages = "461-468"
}

Ristić, N., Ajdžanović, V., Manojlović-Stojanoski, M., Maliković, J., Ušćebrka, G., Marković, Z.,& Milošević, V.. (2017). Effects of estradiol on histological parameters and secretory ability of pituitary mammotrophs in ovariectomized female rats. in Cell Journal, 19(3), 461-468.
https://doi.org/10.22074/cellj.2017.4334

Ristić N, Ajdžanović V, Manojlović-Stojanoski M, Maliković J, Ušćebrka G, Marković Z, Milošević V. Effects of estradiol on histological parameters and secretory ability of pituitary mammotrophs in ovariectomized female rats. in Cell Journal. 2017;19(3):461-468.
doi:10.22074/cellj.2017.4334 .

Ristić, Nataša, Ajdžanović, Vladimir, Manojlović-Stojanoski, Milica, Maliković, Jovana, Ušćebrka, Gordana, Marković, Zorica, Milošević, Verica, "Effects of estradiol on histological parameters and secretory ability of pituitary mammotrophs in ovariectomized female rats" in Cell Journal, 19, no. 3 (2017):461-468,
https://doi.org/10.22074/cellj.2017.4334 . .

TY  - CONF
AU  - Šošić-Jurjević, Branka
AU  - Filipović, Branko
AU  - Živanović, Jasmina
AU  - Ušćebrka, Gordana
AU  - Trifunović, Svetlana
AU  - Ajdžanović, Vladimir
AU  - Ristić, Nataša
AU  - Milošević, Verica
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6377
AB  - Elevated blood level of vitamin D is associated with multiple health benefits. However, vitamin D deficiency is common among the elderly population. Despite multiple cellular targets in both adjacent thyroid C cells and chief parathyroid cells, little is known about how vitamin D affects thyroid follicles and their function in thyroid hormone production. In this study we administered 50mg of vitamin D3 (that corresponds to 2000IU/daily), during three weeks, to orchidectomized middle-aged male rats, which we used as a model of andropause with osteoporosis. After animals’ decapitation, thyroid tissues were histologically analyzed and serum concentrations of total T4 and TSH were determined by the corresponding rat ELISAs. In comparison with the controls, which received the same amount of the sterile olive oil, thyroids of Vit.D3-treated rats were characterized by interstitial C cell hyperplasia, while the follicular tissue remained preserved.
PB  - Basel: Karger
C3  - Programme and Abstract: 39th Annual Meeting of the European Thyroid Association; 2016 Sep 3-6; Copenhagen, Denmark
T1  - Cholecalciferol (Vit. D3) affects thyroid histology and function in orchidectomized middle-aged males rats
DO  - 10.1159/000447416
SP  - 122
ER  - 

@conference{
author = "Šošić-Jurjević, Branka and Filipović, Branko and Živanović, Jasmina and Ušćebrka, Gordana and Trifunović, Svetlana and Ajdžanović, Vladimir and Ristić, Nataša and Milošević, Verica",
year = "2016",
abstract = "Elevated blood level of vitamin D is associated with multiple health benefits. However, vitamin D deficiency is common among the elderly population. Despite multiple cellular targets in both adjacent thyroid C cells and chief parathyroid cells, little is known about how vitamin D affects thyroid follicles and their function in thyroid hormone production. In this study we administered 50mg of vitamin D3 (that corresponds to 2000IU/daily), during three weeks, to orchidectomized middle-aged male rats, which we used as a model of andropause with osteoporosis. After animals’ decapitation, thyroid tissues were histologically analyzed and serum concentrations of total T4 and TSH were determined by the corresponding rat ELISAs. In comparison with the controls, which received the same amount of the sterile olive oil, thyroids of Vit.D3-treated rats were characterized by interstitial C cell hyperplasia, while the follicular tissue remained preserved.",
publisher = "Basel: Karger",
journal = "Programme and Abstract: 39th Annual Meeting of the European Thyroid Association; 2016 Sep 3-6; Copenhagen, Denmark",
title = "Cholecalciferol (Vit. D3) affects thyroid histology and function in orchidectomized middle-aged males rats",
doi = "10.1159/000447416",
pages = "122"
}

Šošić-Jurjević, B., Filipović, B., Živanović, J., Ušćebrka, G., Trifunović, S., Ajdžanović, V., Ristić, N.,& Milošević, V.. (2016). Cholecalciferol (Vit. D3) affects thyroid histology and function in orchidectomized middle-aged males rats. in Programme and Abstract: 39th Annual Meeting of the European Thyroid Association; 2016 Sep 3-6; Copenhagen, Denmark
Basel: Karger., 122.
https://doi.org/10.1159/000447416

Šošić-Jurjević B, Filipović B, Živanović J, Ušćebrka G, Trifunović S, Ajdžanović V, Ristić N, Milošević V. Cholecalciferol (Vit. D3) affects thyroid histology and function in orchidectomized middle-aged males rats. in Programme and Abstract: 39th Annual Meeting of the European Thyroid Association; 2016 Sep 3-6; Copenhagen, Denmark. 2016;:122.
doi:10.1159/000447416 .

Šošić-Jurjević, Branka, Filipović, Branko, Živanović, Jasmina, Ušćebrka, Gordana, Trifunović, Svetlana, Ajdžanović, Vladimir, Ristić, Nataša, Milošević, Verica, "Cholecalciferol (Vit. D3) affects thyroid histology and function in orchidectomized middle-aged males rats" in Programme and Abstract: 39th Annual Meeting of the European Thyroid Association; 2016 Sep 3-6; Copenhagen, Denmark (2016):122,
https://doi.org/10.1159/000447416 . .

TY  - CONF
AU  - Ajdžanović, Vladimir
AU  - Šošić-Jurjević, Branka
AU  - Filipović, Branko
AU  - Popovska-Perčinić, Florina
AU  - Jarić, Ivana
AU  - Miler, Marko
AU  - Ušćebrka, Gordana
AU  - Milošević, Verica
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6280
AB  - Introduction: Genistein is widely used as an alternative therapeutic for various pathological conditions in ageing male and exerts an inhibitory effect on the steroidogenic enzyme families, thus affecting hormonal homeostasis. The aim of this study was to examine the effects of genistein on histological features of the adrenal cortex zona reticularis and the following dehydroepiandrosterone (DHEA) secretion in orchidectomized adult male rats, the most accessible model reflecting andropause.
Material and Methods: The experimental adult Wistar male rats were divided into three groups (n=7): sham-operated (SO), orchidectomized (Orx) and genistein-treated orchidectomized (Orx+G) groups. Genistein (30 mg/kg b.m./day) was administered subcutaneously for three weeks, while the control groups received the vehicle alone. All animal were sacrificed by decapitation 24h after the last injection. The adrenal cortex was analyzed histologically, while serum concentration of DHEA was determined by immunoassay.
Results: Histological analysis revealed that there were no significant changes in adrenal cortex zona reticularis after orchidectomy and orchidectomy followed with genistein treatment. Circulating DHEA concentration was significantly (p<0.005) increased by 97.2% in Orx animals compared to the SO group. In Orx+G group serum DHEA was significantly (p<0.005) decreased by 41.5% in comparison with Orx rats. Serum concentration of DHEA upon genistein application to orchidectomized animals was insignificantly (p>0.05) changed by 15.3% when compared to the SO group.
Conclusion: These data, observed in orchidectomized adult rats, indicate that genistein has an inhibitory effect on the adrenal cortex zona reticularis secretory function.
PB  - Skopje: Ss. Cyril and Methodius University in Skopje, Faculty of Veterinary Medicine
C3  - Proceedings: Days of Veterinary Medicine 2016: 7th International Scientific Meeting; 2016 Sep 22-24; Struga, Republic of Macedonia
T1  - Adrenal zona reticularis in adult orchidectomized male rats after treatment with genistein
SP  - 130
EP  - 131
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6280
ER  - 

@conference{
author = "Ajdžanović, Vladimir and Šošić-Jurjević, Branka and Filipović, Branko and Popovska-Perčinić, Florina and Jarić, Ivana and Miler, Marko and Ušćebrka, Gordana and Milošević, Verica",
year = "2016",
abstract = "Introduction: Genistein is widely used as an alternative therapeutic for various pathological conditions in ageing male and exerts an inhibitory effect on the steroidogenic enzyme families, thus affecting hormonal homeostasis. The aim of this study was to examine the effects of genistein on histological features of the adrenal cortex zona reticularis and the following dehydroepiandrosterone (DHEA) secretion in orchidectomized adult male rats, the most accessible model reflecting andropause.
Material and Methods: The experimental adult Wistar male rats were divided into three groups (n=7): sham-operated (SO), orchidectomized (Orx) and genistein-treated orchidectomized (Orx+G) groups. Genistein (30 mg/kg b.m./day) was administered subcutaneously for three weeks, while the control groups received the vehicle alone. All animal were sacrificed by decapitation 24h after the last injection. The adrenal cortex was analyzed histologically, while serum concentration of DHEA was determined by immunoassay.
Results: Histological analysis revealed that there were no significant changes in adrenal cortex zona reticularis after orchidectomy and orchidectomy followed with genistein treatment. Circulating DHEA concentration was significantly (p<0.005) increased by 97.2% in Orx animals compared to the SO group. In Orx+G group serum DHEA was significantly (p<0.005) decreased by 41.5% in comparison with Orx rats. Serum concentration of DHEA upon genistein application to orchidectomized animals was insignificantly (p>0.05) changed by 15.3% when compared to the SO group.
Conclusion: These data, observed in orchidectomized adult rats, indicate that genistein has an inhibitory effect on the adrenal cortex zona reticularis secretory function.",
publisher = "Skopje: Ss. Cyril and Methodius University in Skopje, Faculty of Veterinary Medicine",
journal = "Proceedings: Days of Veterinary Medicine 2016: 7th International Scientific Meeting; 2016 Sep 22-24; Struga, Republic of Macedonia",
title = "Adrenal zona reticularis in adult orchidectomized male rats after treatment with genistein",
pages = "130-131",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6280"
}

Ajdžanović, V., Šošić-Jurjević, B., Filipović, B., Popovska-Perčinić, F., Jarić, I., Miler, M., Ušćebrka, G.,& Milošević, V.. (2016). Adrenal zona reticularis in adult orchidectomized male rats after treatment with genistein. in Proceedings: Days of Veterinary Medicine 2016: 7th International Scientific Meeting; 2016 Sep 22-24; Struga, Republic of Macedonia
Skopje: Ss. Cyril and Methodius University in Skopje, Faculty of Veterinary Medicine., 130-131.
https://hdl.handle.net/21.15107/rcub_ibiss_6280

Ajdžanović V, Šošić-Jurjević B, Filipović B, Popovska-Perčinić F, Jarić I, Miler M, Ušćebrka G, Milošević V. Adrenal zona reticularis in adult orchidectomized male rats after treatment with genistein. in Proceedings: Days of Veterinary Medicine 2016: 7th International Scientific Meeting; 2016 Sep 22-24; Struga, Republic of Macedonia. 2016;:130-131.
https://hdl.handle.net/21.15107/rcub_ibiss_6280 .

Ajdžanović, Vladimir, Šošić-Jurjević, Branka, Filipović, Branko, Popovska-Perčinić, Florina, Jarić, Ivana, Miler, Marko, Ušćebrka, Gordana, Milošević, Verica, "Adrenal zona reticularis in adult orchidectomized male rats after treatment with genistein" in Proceedings: Days of Veterinary Medicine 2016: 7th International Scientific Meeting; 2016 Sep 22-24; Struga, Republic of Macedonia (2016):130-131,
https://hdl.handle.net/21.15107/rcub_ibiss_6280 .

TY  - JOUR
AU  - Žikić, Dragan
AU  - Stojanović, Slobodan
AU  - Đukić-Stojčić, Mirjana
AU  - Kanački, Zdenko
AU  - Milošević, Verica
AU  - Ušćebrka, Gordana
PY  - 2016
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=1450-91561601027Z
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2602
AB  - Morphological characteristics of skeletal muscles of slow- and medium-growing strains of chickens are very important for meat quality and comparison with fast-growing strains. The aim of this paper was to evaluate morphological parameters of breast and thigh muscles of slow- and mediumgrowing strains in a free-range system. The slow-growing strains used in the experiment were autochthonous breeds Sombor crested and Banat naked neck, and the medium-growing strain was Red-bro. The tissue samples were taken from the thigh muscle and muscles of the breast of 10 chickens of each breed. Samples were stained with hematoxylin - eosin and enzyme succinate - dehydrogenase (SDH). The following morphological parameters were observed: diameter of muscle cells, nucleo-cytoplasmic ratio of muscle cells, volume density of connective tissue within the muscle and the presence of red, white and intermediate muscle cell types. Between strains, the type of muscle or genotype didn’t have significant effects on diameters of muscle cells and nucleo-cytoplasmic ratio in the muscle cells. Results indicated that genotype had significant effect on volume density of the connective tissue in breast muscles. Red muscle cells were, in all strains, significantly more represented in m. biceps femoris than m. pectoralis superficialis. Genotype had significant effect on ratio between connective tissue and muscle cells and no significant effects on other morphological parameters. [Projekat Ministarstva nauke Republike Srbije, br. TR 31033]
T2  - Biotechnology in Animal Husbandry
T1  - Morphological characteristics of breast and thigh muscles of slow- and medium growing strains of chickens
IS  - 1
VL  - 32
DO  - 10.2298/BAH1601027Z
SP  - 27
EP  - 35
ER  - 

@article{
author = "Žikić, Dragan and Stojanović, Slobodan and Đukić-Stojčić, Mirjana and Kanački, Zdenko and Milošević, Verica and Ušćebrka, Gordana",
year = "2016",
abstract = "Morphological characteristics of skeletal muscles of slow- and medium-growing strains of chickens are very important for meat quality and comparison with fast-growing strains. The aim of this paper was to evaluate morphological parameters of breast and thigh muscles of slow- and mediumgrowing strains in a free-range system. The slow-growing strains used in the experiment were autochthonous breeds Sombor crested and Banat naked neck, and the medium-growing strain was Red-bro. The tissue samples were taken from the thigh muscle and muscles of the breast of 10 chickens of each breed. Samples were stained with hematoxylin - eosin and enzyme succinate - dehydrogenase (SDH). The following morphological parameters were observed: diameter of muscle cells, nucleo-cytoplasmic ratio of muscle cells, volume density of connective tissue within the muscle and the presence of red, white and intermediate muscle cell types. Between strains, the type of muscle or genotype didn’t have significant effects on diameters of muscle cells and nucleo-cytoplasmic ratio in the muscle cells. Results indicated that genotype had significant effect on volume density of the connective tissue in breast muscles. Red muscle cells were, in all strains, significantly more represented in m. biceps femoris than m. pectoralis superficialis. Genotype had significant effect on ratio between connective tissue and muscle cells and no significant effects on other morphological parameters. [Projekat Ministarstva nauke Republike Srbije, br. TR 31033]",
journal = "Biotechnology in Animal Husbandry",
title = "Morphological characteristics of breast and thigh muscles of slow- and medium growing strains of chickens",
number = "1",
volume = "32",
doi = "10.2298/BAH1601027Z",
pages = "27-35"
}

Žikić, D., Stojanović, S., Đukić-Stojčić, M., Kanački, Z., Milošević, V.,& Ušćebrka, G.. (2016). Morphological characteristics of breast and thigh muscles of slow- and medium growing strains of chickens. in Biotechnology in Animal Husbandry, 32(1), 27-35.
https://doi.org/10.2298/BAH1601027Z

Žikić D, Stojanović S, Đukić-Stojčić M, Kanački Z, Milošević V, Ušćebrka G. Morphological characteristics of breast and thigh muscles of slow- and medium growing strains of chickens. in Biotechnology in Animal Husbandry. 2016;32(1):27-35.
doi:10.2298/BAH1601027Z .

Žikić, Dragan, Stojanović, Slobodan, Đukić-Stojčić, Mirjana, Kanački, Zdenko, Milošević, Verica, Ušćebrka, Gordana, "Morphological characteristics of breast and thigh muscles of slow- and medium growing strains of chickens" in Biotechnology in Animal Husbandry, 32, no. 1 (2016):27-35,
https://doi.org/10.2298/BAH1601027Z . .

TY  - JOUR
AU  - Milošević, Verica
AU  - Ristić, Nataša
AU  - Todorović, Danijela
AU  - Veličković, Miroslava
AU  - Ušćebrka, Gordana
AU  - Knežević, Veroljub S.
AU  - Ajdžanović, Vladimir
PY  - 2012
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/497
AB  - Estradiol and human chorionic gonadotropin (hCG) are very important in controlling the secretory activity of hormone producing cells in the female rat pituitary glands. The aim of the present study was to examine the morphometric parameters of immunohistochemically la be led ACTH cells in juvenile (16th day) female rat pituitaries after treatment with five doses of estradiol dipropionate (EDP) and two doses of hCG during the neonatal period of life. The controls were treated on the same schedule with an equivalent volume of vehicle. All animals were sacrificed 24 h after the last treatment. ACTH-producing cells were studied using the peroxidase-antiperoxidase immunohistochemical procedure. The absolute and relative pituitary weights were increased (p<0.05) only in the EDP treated group by 120.0% and by 121.1% respectively, in comparison with the controls. In this group, the volume of ACTH cells, volume of their nuclei and volume density were significantly decreased (p<0.05) by 6.4%, 33.3% and 46.2% respectively, compared to the corresponding controls. After treatment with hCG, there were no significant (p>0.05) changes neither in the volume of ACTH cells nor in the volume of their nuclei, in comparison with the controls. On the basis of the results obtained in our study, it can be concluded that EDP, injected into female rats during the neonatal period of life, has an inhibitory effect on the immunohistomorphometric parameters of ACTH cells, but such an effect is not clearly expressed after treatment with hCG.
AB  - Estradiol i humani horionski gonadotropin (hCG) veoma su važni u kontroli sekretorne aktivnosti ćelija hipofize koje proizvode hormone. Cilj ove studije je bio ispitati morfometrijske parametre imunohistohemijski obeleženih ACTH ćelija hipofize ženki pacova, u juvenilnom periodu (16. dan), nakon tretmana sa pet doza estradiol-dipropionata (EDP) ili dve doze humanog horionskog gonadotropina (hCG). Kontrole su bile tretirane na istovetan način sa odgovarajućom količinom rastvarača. Sve životinje su bile žrtvovane 24 h nakon poslednjeg tretmana. ACTH ćelije su bile imunohistohemijski obeležene metodom peroksidaza-antiperoksidaza (PAP). Dobijeni rezultati ukazuju na značajno povećanje (p<0,05) apsolutne i relativne mase hipofize za 120% i 121,1% kod ženki tretiranih sa EDP u odnosu na kontrolu. U ovoj grupi ženki pacova volumen ACTH ćelija i njihovih jedara, kao i njihova volumenska gustina su bili značajno smanjeni (p<0,05) za 6,4%, 33,3% i 46,2% u poređenju sa kontrolom. Nakon tretmana hCG-om, volumen ACTH ćelija, kao i volumen njihovih jedara, nisu bili značajno (p>0,05) promenjeni u odnosu na kontrolu. Na osnovu dobijenih rezultata možemo zaključiti da EDP, injeciran ženkama pacova tokom neonatalnog perioda života, ima inhibitorni efekat na imuno histomorfometrijske parametre ACTH će lija, što nije tako jasno izraženo nakon tretmana hCG-om.
T2  - Journal of Medical Biochemistry
T1  - Imunohistomorfometrijske odlike ACTH ćelija u juvenilnih pacova nakon tretmana estradiolom ili humanim horionskim gonadotropinom
T1  - Immunohistomorphometric features of ACTH cells in juvenile rats after treatment with estradiol or human chorionic gonadotropin
IS  - 1
VL  - 31
SP  - 34
EP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_497
ER  - 

@article{
author = "Milošević, Verica and Ristić, Nataša and Todorović, Danijela and Veličković, Miroslava and Ušćebrka, Gordana and Knežević, Veroljub S. and Ajdžanović, Vladimir",
year = "2012, 2012",
abstract = "Estradiol and human chorionic gonadotropin (hCG) are very important in controlling the secretory activity of hormone producing cells in the female rat pituitary glands. The aim of the present study was to examine the morphometric parameters of immunohistochemically la be led ACTH cells in juvenile (16th day) female rat pituitaries after treatment with five doses of estradiol dipropionate (EDP) and two doses of hCG during the neonatal period of life. The controls were treated on the same schedule with an equivalent volume of vehicle. All animals were sacrificed 24 h after the last treatment. ACTH-producing cells were studied using the peroxidase-antiperoxidase immunohistochemical procedure. The absolute and relative pituitary weights were increased (p<0.05) only in the EDP treated group by 120.0% and by 121.1% respectively, in comparison with the controls. In this group, the volume of ACTH cells, volume of their nuclei and volume density were significantly decreased (p<0.05) by 6.4%, 33.3% and 46.2% respectively, compared to the corresponding controls. After treatment with hCG, there were no significant (p>0.05) changes neither in the volume of ACTH cells nor in the volume of their nuclei, in comparison with the controls. On the basis of the results obtained in our study, it can be concluded that EDP, injected into female rats during the neonatal period of life, has an inhibitory effect on the immunohistomorphometric parameters of ACTH cells, but such an effect is not clearly expressed after treatment with hCG., Estradiol i humani horionski gonadotropin (hCG) veoma su važni u kontroli sekretorne aktivnosti ćelija hipofize koje proizvode hormone. Cilj ove studije je bio ispitati morfometrijske parametre imunohistohemijski obeleženih ACTH ćelija hipofize ženki pacova, u juvenilnom periodu (16. dan), nakon tretmana sa pet doza estradiol-dipropionata (EDP) ili dve doze humanog horionskog gonadotropina (hCG). Kontrole su bile tretirane na istovetan način sa odgovarajućom količinom rastvarača. Sve životinje su bile žrtvovane 24 h nakon poslednjeg tretmana. ACTH ćelije su bile imunohistohemijski obeležene metodom peroksidaza-antiperoksidaza (PAP). Dobijeni rezultati ukazuju na značajno povećanje (p<0,05) apsolutne i relativne mase hipofize za 120% i 121,1% kod ženki tretiranih sa EDP u odnosu na kontrolu. U ovoj grupi ženki pacova volumen ACTH ćelija i njihovih jedara, kao i njihova volumenska gustina su bili značajno smanjeni (p<0,05) za 6,4%, 33,3% i 46,2% u poređenju sa kontrolom. Nakon tretmana hCG-om, volumen ACTH ćelija, kao i volumen njihovih jedara, nisu bili značajno (p>0,05) promenjeni u odnosu na kontrolu. Na osnovu dobijenih rezultata možemo zaključiti da EDP, injeciran ženkama pacova tokom neonatalnog perioda života, ima inhibitorni efekat na imuno histomorfometrijske parametre ACTH će lija, što nije tako jasno izraženo nakon tretmana hCG-om.",
journal = "Journal of Medical Biochemistry",
title = "Imunohistomorfometrijske odlike ACTH ćelija u juvenilnih pacova nakon tretmana estradiolom ili humanim horionskim gonadotropinom, Immunohistomorphometric features of ACTH cells in juvenile rats after treatment with estradiol or human chorionic gonadotropin",
number = "1",
volume = "31",
pages = "34-39",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_497"
}

Milošević, V., Ristić, N., Todorović, D., Veličković, M., Ušćebrka, G., Knežević, V. S.,& Ajdžanović, V.. (2012). Imunohistomorfometrijske odlike ACTH ćelija u juvenilnih pacova nakon tretmana estradiolom ili humanim horionskim gonadotropinom. in Journal of Medical Biochemistry, 31(1), 34-39.
https://hdl.handle.net/21.15107/rcub_ibiss_497

Milošević V, Ristić N, Todorović D, Veličković M, Ušćebrka G, Knežević VS, Ajdžanović V. Imunohistomorfometrijske odlike ACTH ćelija u juvenilnih pacova nakon tretmana estradiolom ili humanim horionskim gonadotropinom. in Journal of Medical Biochemistry. 2012;31(1):34-39.
https://hdl.handle.net/21.15107/rcub_ibiss_497 .

Milošević, Verica, Ristić, Nataša, Todorović, Danijela, Veličković, Miroslava, Ušćebrka, Gordana, Knežević, Veroljub S., Ajdžanović, Vladimir, "Imunohistomorfometrijske odlike ACTH ćelija u juvenilnih pacova nakon tretmana estradiolom ili humanim horionskim gonadotropinom" in Journal of Medical Biochemistry, 31, no. 1 (2012):34-39,
https://hdl.handle.net/21.15107/rcub_ibiss_497 .