TY  - JOUR
AU  - Ehmedah, Adil
AU  - Nedeljković, Predrag
AU  - Dacic, Sanja
AU  - Repac, Jelena
AU  - Drašković Pavlović, Biljana
AU  - Vučević, Dragana
AU  - Peković, Sanja
AU  - Božić Nedeljković, Biljana
PY  - 2019
UR  - https://www.mdpi.com/1420-3049/24/24/4615
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3578
AB  - Peripheral nerve injury (PNI) leads to a series of cellular and molecular events necessary for axon regeneration and reinnervation of target tissues, among which inflammation is crucial for the orchestration of all these processes. Macrophage activation underlies the pathogenesis of PNI and is characterized by morphological/phenotype transformation from proinflammatory (M1) to an anti-inflammatory (M2) type with different functions in the inflammatory and reparative process. The aim of this study was to evaluate influence of the vitamin B (B1, B2, B3, B5, B6, and B12) complex on the process of neuroinflammation that is in part regulated by l-type CaV1.2 calcium channels. A controlled transection of the motor branch of the femoral peripheral nerve was used as an experimental model. Animals were sacrificed after 1, 3, 7, and 14 injections of vitamin B complex. Isolated nerves were used for immunofluorescence analysis. Treatment with vitamin B complex decreased expression of proinflammatory and increased expression of anti-inflammatory cytokines, thus contributing to the resolution of neuroinflammation. In parallel, B vitamins decreased the number of M1 macrophages that expressed the CaV1.2 channel, and increased the number of M2 macrophages that expressed this channel, suggesting their role in M1/M2 transition after PNI. In conclusion, B vitamins had the potential for treatment of neuroinflammation and neuroregeneration and thereby might be an effective therapy for PNI in humans.
PB  - Multidisciplinary Digital Publishing Institute
T2  - Molecules (Basel, Switzerland)
T1  - Vitamin B Complex Treatment Attenuates Local Inflammation after Peripheral Nerve Injury.
IS  - 24
VL  - 24
DO  - 10.3390/molecules24244615
SP  - 4615
ER  - 

@article{
author = "Ehmedah, Adil and Nedeljković, Predrag and Dacic, Sanja and Repac, Jelena and Drašković Pavlović, Biljana and Vučević, Dragana and Peković, Sanja and Božić Nedeljković, Biljana",
year = "2019",
abstract = "Peripheral nerve injury (PNI) leads to a series of cellular and molecular events necessary for axon regeneration and reinnervation of target tissues, among which inflammation is crucial for the orchestration of all these processes. Macrophage activation underlies the pathogenesis of PNI and is characterized by morphological/phenotype transformation from proinflammatory (M1) to an anti-inflammatory (M2) type with different functions in the inflammatory and reparative process. The aim of this study was to evaluate influence of the vitamin B (B1, B2, B3, B5, B6, and B12) complex on the process of neuroinflammation that is in part regulated by l-type CaV1.2 calcium channels. A controlled transection of the motor branch of the femoral peripheral nerve was used as an experimental model. Animals were sacrificed after 1, 3, 7, and 14 injections of vitamin B complex. Isolated nerves were used for immunofluorescence analysis. Treatment with vitamin B complex decreased expression of proinflammatory and increased expression of anti-inflammatory cytokines, thus contributing to the resolution of neuroinflammation. In parallel, B vitamins decreased the number of M1 macrophages that expressed the CaV1.2 channel, and increased the number of M2 macrophages that expressed this channel, suggesting their role in M1/M2 transition after PNI. In conclusion, B vitamins had the potential for treatment of neuroinflammation and neuroregeneration and thereby might be an effective therapy for PNI in humans.",
publisher = "Multidisciplinary Digital Publishing Institute",
journal = "Molecules (Basel, Switzerland)",
title = "Vitamin B Complex Treatment Attenuates Local Inflammation after Peripheral Nerve Injury.",
number = "24",
volume = "24",
doi = "10.3390/molecules24244615",
pages = "4615"
}

Ehmedah, A., Nedeljković, P., Dacic, S., Repac, J., Drašković Pavlović, B., Vučević, D., Peković, S.,& Božić Nedeljković, B.. (2019). Vitamin B Complex Treatment Attenuates Local Inflammation after Peripheral Nerve Injury.. in Molecules (Basel, Switzerland)
Multidisciplinary Digital Publishing Institute., 24(24), 4615.
https://doi.org/10.3390/molecules24244615

Ehmedah A, Nedeljković P, Dacic S, Repac J, Drašković Pavlović B, Vučević D, Peković S, Božić Nedeljković B. Vitamin B Complex Treatment Attenuates Local Inflammation after Peripheral Nerve Injury.. in Molecules (Basel, Switzerland). 2019;24(24):4615.
doi:10.3390/molecules24244615 .

Ehmedah, Adil, Nedeljković, Predrag, Dacic, Sanja, Repac, Jelena, Drašković Pavlović, Biljana, Vučević, Dragana, Peković, Sanja, Božić Nedeljković, Biljana, "Vitamin B Complex Treatment Attenuates Local Inflammation after Peripheral Nerve Injury." in Molecules (Basel, Switzerland), 24, no. 24 (2019):4615,
https://doi.org/10.3390/molecules24244615 . .

TY  - JOUR
AU  - Lavrnja, Irena
AU  - Savić, Danijela
AU  - Parabucki, Ana
AU  - Dacic, Sanja
AU  - Laketa, Danijela
AU  - Peković, Sanja
AU  - Stojiljkovic, Mirjana
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2062
AB  - Compelling evidence now points to the critical role of the cytoskeleton
   in neurodegeneration. In the present study, using an immunohistochemical
   approach, we have shown that cortical stab injury (CSI) in adult Wistar
   rats significantly affects temporal pattern of expression of
   neurofilament proteins (NFs). a major cytoskeleton components of
   neurons, and microtubule-associated proteins (MAP2). At 3 days
   post-injury (dpi) most of the NFs immunoreactivity was found in pyknotic
   neurons and in fragmentized axonal processes in the perilesioned cortex.
   These cytoskeletal alterations became more pronounced by 10 dpi. At the
   subcellular level CSI also showed significant impact on NFs and MAP-2
   expression. Thus, at 3 dpi most of the dendrites disappeared, while
   large neuronal somata appeared like open circles pointing to membrane
   disintegration. Conversely, at 10 dpi neuronal perikarya and a few new
   apical dendrites were strongly labeled. Since aberrant NF
   phosphorylation is a pathological hallmark of many human
   neurodegenerative disorders, as well as is found after stressor stimuli,
   the present results shed light into the expression of neurofilaments
   after the stab brain injury. (C) 2014 Elsevier GmbH. All rights
   reserved.
T2  - Acta Histochemica
T1  - Effect of stab injury in the rat cerebral cortex on temporal pattern of
 expression of neuronal cytoskeletal proteins: An immunohistochemical
 study
IS  - 2
VL  - 117
DO  - 10.1016/j.acthis.2014.12.004
SP  - 155
EP  - 162
ER  - 

@article{
author = "Lavrnja, Irena and Savić, Danijela and Parabucki, Ana and Dacic, Sanja and Laketa, Danijela and Peković, Sanja and Stojiljkovic, Mirjana",
year = "2015",
abstract = "Compelling evidence now points to the critical role of the cytoskeleton
   in neurodegeneration. In the present study, using an immunohistochemical
   approach, we have shown that cortical stab injury (CSI) in adult Wistar
   rats significantly affects temporal pattern of expression of
   neurofilament proteins (NFs). a major cytoskeleton components of
   neurons, and microtubule-associated proteins (MAP2). At 3 days
   post-injury (dpi) most of the NFs immunoreactivity was found in pyknotic
   neurons and in fragmentized axonal processes in the perilesioned cortex.
   These cytoskeletal alterations became more pronounced by 10 dpi. At the
   subcellular level CSI also showed significant impact on NFs and MAP-2
   expression. Thus, at 3 dpi most of the dendrites disappeared, while
   large neuronal somata appeared like open circles pointing to membrane
   disintegration. Conversely, at 10 dpi neuronal perikarya and a few new
   apical dendrites were strongly labeled. Since aberrant NF
   phosphorylation is a pathological hallmark of many human
   neurodegenerative disorders, as well as is found after stressor stimuli,
   the present results shed light into the expression of neurofilaments
   after the stab brain injury. (C) 2014 Elsevier GmbH. All rights
   reserved.",
journal = "Acta Histochemica",
title = "Effect of stab injury in the rat cerebral cortex on temporal pattern of
 expression of neuronal cytoskeletal proteins: An immunohistochemical
 study",
number = "2",
volume = "117",
doi = "10.1016/j.acthis.2014.12.004",
pages = "155-162"
}

Lavrnja, I., Savić, D., Parabucki, A., Dacic, S., Laketa, D., Peković, S.,& Stojiljkovic, M.. (2015). Effect of stab injury in the rat cerebral cortex on temporal pattern of
 expression of neuronal cytoskeletal proteins: An immunohistochemical
 study. in Acta Histochemica, 117(2), 155-162.
https://doi.org/10.1016/j.acthis.2014.12.004

Lavrnja I, Savić D, Parabucki A, Dacic S, Laketa D, Peković S, Stojiljkovic M. Effect of stab injury in the rat cerebral cortex on temporal pattern of
 expression of neuronal cytoskeletal proteins: An immunohistochemical
 study. in Acta Histochemica. 2015;117(2):155-162.
doi:10.1016/j.acthis.2014.12.004 .

Lavrnja, Irena, Savić, Danijela, Parabucki, Ana, Dacic, Sanja, Laketa, Danijela, Peković, Sanja, Stojiljkovic, Mirjana, "Effect of stab injury in the rat cerebral cortex on temporal pattern of
 expression of neuronal cytoskeletal proteins: An immunohistochemical
 study" in Acta Histochemica, 117, no. 2 (2015):155-162,
https://doi.org/10.1016/j.acthis.2014.12.004 . .

TY  - JOUR
AU  - Lavrnja, Irena
AU  - Parabucki, Ana
AU  - Brkić, Predrag
AU  - Jovanović, Tomislav
AU  - Dacic, Sanja
AU  - Savić, Danijela
AU  - Pantić, Igor
AU  - Stojiljković, Mirjana
AU  - Peković, Sanja
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2070
AB  - The exact mechanisms by which treatment with hyperbaric oxygen (HBOT)
   exerts its beneficial effects on recovery after brain injury are still
   unrevealed. Therefore, in this study we investigated the influence of
   repetitive HBOT on the reactive astrogliosis and expression of mediators
   of inflammation after cortical stab injury (CSI). CSI was performed on
   male Wistar rats, divided into control, sham, and lesioned groups with
   appropriate HBO. The HBOT protocol was as follows: 10 minutes of slow
   compression, 2.5 atmospheres absolute (ATA) for 60 minutes, and 10
   minutes of slow decompression, once a day for 10 consecutive days. Data
   obtained using real-time polymerase chain reaction, Western blot, and
   immunohistochemical and immunofluorescence analyses revealed that
   repetitive HBOT applied after the CSI attenuates reactive astrogliosis
   and glial scarring, and reduces expression of GFAP (glial fibrillary
   acidic protein), vimentin, and ICAM-1 (intercellular adhesion
   molecule-1) both at gene and tissue levels. In addition, HBOT prevents
   expression of CD40 and its ligand CD40L on microglia, neutrophils,
   cortical neurons, and reactive astrocytes. Accordingly, repetitive HBOT,
   by prevention of glial scarring and limiting of expression of
   inflammatory mediators, supports formation of more permissive
   environment for repair and regeneration.
T2  - Mediators of Inflammation
T1  - Repetitive Hyperbaric Oxygenation Attenuates Reactive Astrogliosis and
 Suppresses Expression of Inflammatory Mediators in the Rat Model of
 Brain Injury
IS  - 498405
DO  - 10.1155/2015/498405
ER  - 

@article{
author = "Lavrnja, Irena and Parabucki, Ana and Brkić, Predrag and Jovanović, Tomislav and Dacic, Sanja and Savić, Danijela and Pantić, Igor and Stojiljković, Mirjana and Peković, Sanja",
year = "2015",
abstract = "The exact mechanisms by which treatment with hyperbaric oxygen (HBOT)
   exerts its beneficial effects on recovery after brain injury are still
   unrevealed. Therefore, in this study we investigated the influence of
   repetitive HBOT on the reactive astrogliosis and expression of mediators
   of inflammation after cortical stab injury (CSI). CSI was performed on
   male Wistar rats, divided into control, sham, and lesioned groups with
   appropriate HBO. The HBOT protocol was as follows: 10 minutes of slow
   compression, 2.5 atmospheres absolute (ATA) for 60 minutes, and 10
   minutes of slow decompression, once a day for 10 consecutive days. Data
   obtained using real-time polymerase chain reaction, Western blot, and
   immunohistochemical and immunofluorescence analyses revealed that
   repetitive HBOT applied after the CSI attenuates reactive astrogliosis
   and glial scarring, and reduces expression of GFAP (glial fibrillary
   acidic protein), vimentin, and ICAM-1 (intercellular adhesion
   molecule-1) both at gene and tissue levels. In addition, HBOT prevents
   expression of CD40 and its ligand CD40L on microglia, neutrophils,
   cortical neurons, and reactive astrocytes. Accordingly, repetitive HBOT,
   by prevention of glial scarring and limiting of expression of
   inflammatory mediators, supports formation of more permissive
   environment for repair and regeneration.",
journal = "Mediators of Inflammation",
title = "Repetitive Hyperbaric Oxygenation Attenuates Reactive Astrogliosis and
 Suppresses Expression of Inflammatory Mediators in the Rat Model of
 Brain Injury",
number = "498405",
doi = "10.1155/2015/498405"
}

Lavrnja, I., Parabucki, A., Brkić, P., Jovanović, T., Dacic, S., Savić, D., Pantić, I., Stojiljković, M.,& Peković, S.. (2015). Repetitive Hyperbaric Oxygenation Attenuates Reactive Astrogliosis and
 Suppresses Expression of Inflammatory Mediators in the Rat Model of
 Brain Injury. in Mediators of Inflammation(498405).
https://doi.org/10.1155/2015/498405

Lavrnja I, Parabucki A, Brkić P, Jovanović T, Dacic S, Savić D, Pantić I, Stojiljković M, Peković S. Repetitive Hyperbaric Oxygenation Attenuates Reactive Astrogliosis and
 Suppresses Expression of Inflammatory Mediators in the Rat Model of
 Brain Injury. in Mediators of Inflammation. 2015;(498405).
doi:10.1155/2015/498405 .

Lavrnja, Irena, Parabucki, Ana, Brkić, Predrag, Jovanović, Tomislav, Dacic, Sanja, Savić, Danijela, Pantić, Igor, Stojiljković, Mirjana, Peković, Sanja, "Repetitive Hyperbaric Oxygenation Attenuates Reactive Astrogliosis and
 Suppresses Expression of Inflammatory Mediators in the Rat Model of
 Brain Injury" in Mediators of Inflammation, no. 498405 (2015),
https://doi.org/10.1155/2015/498405 . .

TY  - JOUR
AU  - Pantic, Igor
AU  - Dacic, Sanja
AU  - Brkic, Predrag
AU  - Lavrnja, Irena
AU  - Jovanovic, Tomislav
AU  - Pantic, Senka
AU  - Peković, Sanja
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1972
AB  - Fractal and grey level co-occurrence matrix (GLCM) analysis represent
   two mathematical computer-assisted algorithms that are today thought to
   be able to accurately detect and quantify changes in tissue architecture
   during various physiological and pathological processes. However,
   despite their numerous applications in histology and pathology, their
   sensitivity, specificity and validity regarding evaluation of brain
   tissue remain unclear. In this article we present the results indicating
   that certain parameters of fractal and GLCM analysis have high
   discriminatory ability in distinguishing two morphologically similar
   regions of rat hippocampus: stratum lacunosum-moleculare and stratum
   radiatum. Fractal and GLCM algorithms were performed on a total of 240
   thionine-stained hippocampus micrographs of 12 male Wistar albino rats.
   120 digital micrographs represented stratum lacunosum-moleculare, and
   another 120 stratum radiatum. For each image, 7 parameters were
   calculated: fractal dimension, lacunarity, GLCM angular second moment,
   GLCM contrast, inverse difference moment, GLCM correlation, and GLCM
   variance. GLCM variance (VAR) resulted in the largest area under the
   Receiver operating characteristic (ROC) curve of 0.96, demonstrating an
   outstanding discriminatory power in analysis of stratum
   lacunosum-moleculare (average VAR equaled 478.1 +/- 179.8) and stratum
   radiatum (average VAR of 145.9 +/- 59.2, p <0.0001). For the criterion
   VAR <= 227.5, sensitivity and specificity were 90\% and 86.7\%,
   respectively. GLCM correlation as a parameter also produced large area
   under the ROC curve of 0.95. Our results are in accordance with the
   findings of our previous study regarding brain white mass fractal and
   textural analysis. GLCM algorithm as an image analysis method has
   potentially high applicability in structural analysis of brain tissue
   cytoarcitecture. (C) 2015 Elsevier Ltd. All rights reserved.
T2  - Journal of Theoretical Biology
T1  - Discriminatory ability of fractal and grey level co-occurrence matrix
 methods in structural analysis of hippocampus layers
VL  - 370
DO  - 10.1016/j.jtbi.2015.01.035
SP  - 151
EP  - 156
ER  - 

@article{
author = "Pantic, Igor and Dacic, Sanja and Brkic, Predrag and Lavrnja, Irena and Jovanovic, Tomislav and Pantic, Senka and Peković, Sanja",
year = "2015",
abstract = "Fractal and grey level co-occurrence matrix (GLCM) analysis represent
   two mathematical computer-assisted algorithms that are today thought to
   be able to accurately detect and quantify changes in tissue architecture
   during various physiological and pathological processes. However,
   despite their numerous applications in histology and pathology, their
   sensitivity, specificity and validity regarding evaluation of brain
   tissue remain unclear. In this article we present the results indicating
   that certain parameters of fractal and GLCM analysis have high
   discriminatory ability in distinguishing two morphologically similar
   regions of rat hippocampus: stratum lacunosum-moleculare and stratum
   radiatum. Fractal and GLCM algorithms were performed on a total of 240
   thionine-stained hippocampus micrographs of 12 male Wistar albino rats.
   120 digital micrographs represented stratum lacunosum-moleculare, and
   another 120 stratum radiatum. For each image, 7 parameters were
   calculated: fractal dimension, lacunarity, GLCM angular second moment,
   GLCM contrast, inverse difference moment, GLCM correlation, and GLCM
   variance. GLCM variance (VAR) resulted in the largest area under the
   Receiver operating characteristic (ROC) curve of 0.96, demonstrating an
   outstanding discriminatory power in analysis of stratum
   lacunosum-moleculare (average VAR equaled 478.1 +/- 179.8) and stratum
   radiatum (average VAR of 145.9 +/- 59.2, p <0.0001). For the criterion
   VAR <= 227.5, sensitivity and specificity were 90\% and 86.7\%,
   respectively. GLCM correlation as a parameter also produced large area
   under the ROC curve of 0.95. Our results are in accordance with the
   findings of our previous study regarding brain white mass fractal and
   textural analysis. GLCM algorithm as an image analysis method has
   potentially high applicability in structural analysis of brain tissue
   cytoarcitecture. (C) 2015 Elsevier Ltd. All rights reserved.",
journal = "Journal of Theoretical Biology",
title = "Discriminatory ability of fractal and grey level co-occurrence matrix
 methods in structural analysis of hippocampus layers",
volume = "370",
doi = "10.1016/j.jtbi.2015.01.035",
pages = "151-156"
}

Pantic, I., Dacic, S., Brkic, P., Lavrnja, I., Jovanovic, T., Pantic, S.,& Peković, S.. (2015). Discriminatory ability of fractal and grey level co-occurrence matrix
 methods in structural analysis of hippocampus layers. in Journal of Theoretical Biology, 370, 151-156.
https://doi.org/10.1016/j.jtbi.2015.01.035

Pantic I, Dacic S, Brkic P, Lavrnja I, Jovanovic T, Pantic S, Peković S. Discriminatory ability of fractal and grey level co-occurrence matrix
 methods in structural analysis of hippocampus layers. in Journal of Theoretical Biology. 2015;370:151-156.
doi:10.1016/j.jtbi.2015.01.035 .

Pantic, Igor, Dacic, Sanja, Brkic, Predrag, Lavrnja, Irena, Jovanovic, Tomislav, Pantic, Senka, Peković, Sanja, "Discriminatory ability of fractal and grey level co-occurrence matrix
 methods in structural analysis of hippocampus layers" in Journal of Theoretical Biology, 370 (2015):151-156,
https://doi.org/10.1016/j.jtbi.2015.01.035 . .

TY  - JOUR
AU  - Savić, Danijela
AU  - Lavrnja, Irena
AU  - Dacic, Sanja
AU  - Bjelobaba, Ivana
AU  - Nedeljkovic, Nadezda
AU  - Stojiljkovic, Mirjana
AU  - Herdegen, Thomas
AU  - Peković, Sanja
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2263
AB  - Tiazofurin is a purine nucleoside analogue, with a broad spectrum of
   antitumoral and anti-inflammatory properties. In the present study, we
   have investigated the effect of tiazofurin on microglial inflammatory
   response to lipopolysaccharide in vitro. The cytotoxic effect of the
   drug was examined by sulforhodamine B assay. The Griess method was used
   to quantify nitrite production. Microglial morphology was assessed by
   measuring cell body size. Release of the pro-inflammatory cytokines,
   tumor necrosis factor-alpha, interleukin-1 beta, interleukin-6, and the
   anti-inflammatory cytokine interleukin-10, were evaluated by
   enzyme-linked immunosorbent assay. Our data showed that tiazofurin
   decreased the number of activated microglia, lowered nitric oxide
   production and reduced the average cell surface of these cells.
   Tiazofurin reduced tumor necrosis factor-alpha, interleukin-6 and
   increased interleukin-10 secretion. Conversely, this drug promoted the
   release of interleukin-1 beta. Results obtained in this study indicate
   that TR displayed both anti-and pro-inflammatory modulation of activated
   microglia that could be relevant for its antitumor action within the
   central nervous system.
T2  - Archives of Biological Sciences
T1  - Tiazofurin modulates lipopolysaccharide-activated microglia in vitro
IS  - 4
VL  - 66
DO  - 10.2298/ABS1404633S
SP  - 1633
EP  - 1640
ER  - 

@article{
author = "Savić, Danijela and Lavrnja, Irena and Dacic, Sanja and Bjelobaba, Ivana and Nedeljkovic, Nadezda and Stojiljkovic, Mirjana and Herdegen, Thomas and Peković, Sanja",
year = "2014",
abstract = "Tiazofurin is a purine nucleoside analogue, with a broad spectrum of
   antitumoral and anti-inflammatory properties. In the present study, we
   have investigated the effect of tiazofurin on microglial inflammatory
   response to lipopolysaccharide in vitro. The cytotoxic effect of the
   drug was examined by sulforhodamine B assay. The Griess method was used
   to quantify nitrite production. Microglial morphology was assessed by
   measuring cell body size. Release of the pro-inflammatory cytokines,
   tumor necrosis factor-alpha, interleukin-1 beta, interleukin-6, and the
   anti-inflammatory cytokine interleukin-10, were evaluated by
   enzyme-linked immunosorbent assay. Our data showed that tiazofurin
   decreased the number of activated microglia, lowered nitric oxide
   production and reduced the average cell surface of these cells.
   Tiazofurin reduced tumor necrosis factor-alpha, interleukin-6 and
   increased interleukin-10 secretion. Conversely, this drug promoted the
   release of interleukin-1 beta. Results obtained in this study indicate
   that TR displayed both anti-and pro-inflammatory modulation of activated
   microglia that could be relevant for its antitumor action within the
   central nervous system.",
journal = "Archives of Biological Sciences",
title = "Tiazofurin modulates lipopolysaccharide-activated microglia in vitro",
number = "4",
volume = "66",
doi = "10.2298/ABS1404633S",
pages = "1633-1640"
}

Savić, D., Lavrnja, I., Dacic, S., Bjelobaba, I., Nedeljkovic, N., Stojiljkovic, M., Herdegen, T.,& Peković, S.. (2014). Tiazofurin modulates lipopolysaccharide-activated microglia in vitro. in Archives of Biological Sciences, 66(4), 1633-1640.
https://doi.org/10.2298/ABS1404633S

Savić D, Lavrnja I, Dacic S, Bjelobaba I, Nedeljkovic N, Stojiljkovic M, Herdegen T, Peković S. Tiazofurin modulates lipopolysaccharide-activated microglia in vitro. in Archives of Biological Sciences. 2014;66(4):1633-1640.
doi:10.2298/ABS1404633S .

Savić, Danijela, Lavrnja, Irena, Dacic, Sanja, Bjelobaba, Ivana, Nedeljkovic, Nadezda, Stojiljkovic, Mirjana, Herdegen, Thomas, Peković, Sanja, "Tiazofurin modulates lipopolysaccharide-activated microglia in vitro" in Archives of Biological Sciences, 66, no. 4 (2014):1633-1640,
https://doi.org/10.2298/ABS1404633S . .

TY  - JOUR
AU  - Pantic, Igor
AU  - Dacic, Sanja
AU  - Brkic, Predrag
AU  - Lavrnja, Irena
AU  - Pantic, Senka
AU  - Jovanovic, Tomislav
AU  - Peković, Sanja
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2144
AB  - This aim of this study was to assess the discriminatory value of fractal
   and grey level co-occurrence matrix (GLCM) analysis methods in standard
   microscopy analysis of two histologically similar brain white mass
   regions that have different nerve fiber orientation. A total of 160
   digital micrographs of thionine-stained rat brain white mass were
   acquired using a Pro-MicroScan DEM-200 instrument. Eighty micrographs
   from the anterior corpus callosum and eighty from the anterior cingulum
   areas of the brain were analyzed. The micrographs were evaluated using
   the National Institutes of Health ImageJ software and its plugins. For
   each micrograph, seven parameters were calculated: angular second
   moment, inverse difference moment, GLCM contrast, GLCM correlation, GLCM
   variance, fractal dimension, and lacunarity. Using the Receiver
   operating characteristic analysis, the highest discriminatory value was
   determined for inverse difference moment (IDM) (area under the receiver
   operating characteristic (ROC) curve equaled 0.925, and for the
   criterion IDM <= 0.610 the sensitivity and specificity were 82.5 and
   87.5\%, respectively). Most of the other parameters also showed good
   sensitivity and specificity. The results indicate that GLCM and fractal
   analysis methods, when applied together in brain histology analysis, are
   highly capable of discriminating white mass structures that have
   different axonal orientation.
T2  - Microscopy and Microanalysis
T1  - Application of Fractal and Grey Level Co-Occurrence Matrix Analysis in
 Evaluation of Brain Corpus Callosum and Cingulum Architecture
IS  - 5
VL  - 20
DO  - 10.1017/S1431927614012811
SP  - 1373
EP  - 1381
ER  - 

@article{
author = "Pantic, Igor and Dacic, Sanja and Brkic, Predrag and Lavrnja, Irena and Pantic, Senka and Jovanovic, Tomislav and Peković, Sanja",
year = "2014",
abstract = "This aim of this study was to assess the discriminatory value of fractal
   and grey level co-occurrence matrix (GLCM) analysis methods in standard
   microscopy analysis of two histologically similar brain white mass
   regions that have different nerve fiber orientation. A total of 160
   digital micrographs of thionine-stained rat brain white mass were
   acquired using a Pro-MicroScan DEM-200 instrument. Eighty micrographs
   from the anterior corpus callosum and eighty from the anterior cingulum
   areas of the brain were analyzed. The micrographs were evaluated using
   the National Institutes of Health ImageJ software and its plugins. For
   each micrograph, seven parameters were calculated: angular second
   moment, inverse difference moment, GLCM contrast, GLCM correlation, GLCM
   variance, fractal dimension, and lacunarity. Using the Receiver
   operating characteristic analysis, the highest discriminatory value was
   determined for inverse difference moment (IDM) (area under the receiver
   operating characteristic (ROC) curve equaled 0.925, and for the
   criterion IDM <= 0.610 the sensitivity and specificity were 82.5 and
   87.5\%, respectively). Most of the other parameters also showed good
   sensitivity and specificity. The results indicate that GLCM and fractal
   analysis methods, when applied together in brain histology analysis, are
   highly capable of discriminating white mass structures that have
   different axonal orientation.",
journal = "Microscopy and Microanalysis",
title = "Application of Fractal and Grey Level Co-Occurrence Matrix Analysis in
 Evaluation of Brain Corpus Callosum and Cingulum Architecture",
number = "5",
volume = "20",
doi = "10.1017/S1431927614012811",
pages = "1373-1381"
}

Pantic, I., Dacic, S., Brkic, P., Lavrnja, I., Pantic, S., Jovanovic, T.,& Peković, S.. (2014). Application of Fractal and Grey Level Co-Occurrence Matrix Analysis in
 Evaluation of Brain Corpus Callosum and Cingulum Architecture. in Microscopy and Microanalysis, 20(5), 1373-1381.
https://doi.org/10.1017/S1431927614012811

Pantic I, Dacic S, Brkic P, Lavrnja I, Pantic S, Jovanovic T, Peković S. Application of Fractal and Grey Level Co-Occurrence Matrix Analysis in
 Evaluation of Brain Corpus Callosum and Cingulum Architecture. in Microscopy and Microanalysis. 2014;20(5):1373-1381.
doi:10.1017/S1431927614012811 .

Pantic, Igor, Dacic, Sanja, Brkic, Predrag, Lavrnja, Irena, Pantic, Senka, Jovanovic, Tomislav, Peković, Sanja, "Application of Fractal and Grey Level Co-Occurrence Matrix Analysis in
 Evaluation of Brain Corpus Callosum and Cingulum Architecture" in Microscopy and Microanalysis, 20, no. 5 (2014):1373-1381,
https://doi.org/10.1017/S1431927614012811 . .