TY  - JOUR
AU  - Petropoulos, Spyridon A
AU  - Taofiq, Oludemi
AU  - Fernandes, Ângela
AU  - Tzortzakis, Nikos
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Ferreira, Isabel CFR
PY  - 2019
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1002/jsfa.9881
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3445
AB  - BACKGROUND The scarcity of irrigation water is severely affecting global crop production. In this context, biostimulants are increasingly used as alternatives means against abiotic stress conditions. In this study, phenolic compounds composition and bioactive properties of common bean (Phaseolus vulgaris L.) plants grown under water stress conditions and biostimulants application were investigated. RESULTS Sixteen individual phenolic compounds were detected in both pods and seeds with a notable difference in their compositional profile. A significant effect on phenolic compounds content and composition was also observed for the biostimulants tested. Regarding the antibacterial activity, pods of the second harvest and seed extracts showed significant efficacy against Bacillus cereus, especially in water-stressed plants, where all biostimulant treatments were more effective than positive controls. Moreover, all biostimulant treatments for seed extracts of water-stressed plants were more effective against Staphylococcus aureus compared with ampicillin, whereas streptomycin showed the best results. Extracts from pods of the second harvest from normally irrigated plants showed the best results against the fungi tested, except for Penicillium verrucosum var. cyclopium. Finally, no significant cytotoxic effects were detected. CONCLUSION In conclusion, the biostimulants tested increased total phenolic compounds content compared with control treatment, especially in pods of the first harvest and seeds of water-stressed plants. Moreover, bioactive properties showed a varied response in regard to irrigation and biostimulant treatment. Therefore, biostimulants can be considered as a useful means towards increasing phenolic compounds content, and they may also affect the antimicrobial properties of pods and seeds extracts. © 2019 Society of Chemical Industry.
T2  - Journal of the Science of Food and Agriculture
T1  - Bioactive properties of greenhouse-cultivated green beans (Phaseolus vulgaris L.) under biostimulants and water-stress effect.
DO  - 10.1002/jsfa.9881
ER  - 

@article{
author = "Petropoulos, Spyridon A and Taofiq, Oludemi and Fernandes, Ângela and Tzortzakis, Nikos and Ćirić, Ana and Soković, Marina and Barros, Lillian and Ferreira, Isabel CFR",
year = "2019",
abstract = "BACKGROUND The scarcity of irrigation water is severely affecting global crop production. In this context, biostimulants are increasingly used as alternatives means against abiotic stress conditions. In this study, phenolic compounds composition and bioactive properties of common bean (Phaseolus vulgaris L.) plants grown under water stress conditions and biostimulants application were investigated. RESULTS Sixteen individual phenolic compounds were detected in both pods and seeds with a notable difference in their compositional profile. A significant effect on phenolic compounds content and composition was also observed for the biostimulants tested. Regarding the antibacterial activity, pods of the second harvest and seed extracts showed significant efficacy against Bacillus cereus, especially in water-stressed plants, where all biostimulant treatments were more effective than positive controls. Moreover, all biostimulant treatments for seed extracts of water-stressed plants were more effective against Staphylococcus aureus compared with ampicillin, whereas streptomycin showed the best results. Extracts from pods of the second harvest from normally irrigated plants showed the best results against the fungi tested, except for Penicillium verrucosum var. cyclopium. Finally, no significant cytotoxic effects were detected. CONCLUSION In conclusion, the biostimulants tested increased total phenolic compounds content compared with control treatment, especially in pods of the first harvest and seeds of water-stressed plants. Moreover, bioactive properties showed a varied response in regard to irrigation and biostimulant treatment. Therefore, biostimulants can be considered as a useful means towards increasing phenolic compounds content, and they may also affect the antimicrobial properties of pods and seeds extracts. © 2019 Society of Chemical Industry.",
journal = "Journal of the Science of Food and Agriculture",
title = "Bioactive properties of greenhouse-cultivated green beans (Phaseolus vulgaris L.) under biostimulants and water-stress effect.",
doi = "10.1002/jsfa.9881"
}

Petropoulos, S. A., Taofiq, O., Fernandes, Â., Tzortzakis, N., Ćirić, A., Soković, M., Barros, L.,& Ferreira, I. C.. (2019). Bioactive properties of greenhouse-cultivated green beans (Phaseolus vulgaris L.) under biostimulants and water-stress effect.. in Journal of the Science of Food and Agriculture.
https://doi.org/10.1002/jsfa.9881

Petropoulos SA, Taofiq O, Fernandes Â, Tzortzakis N, Ćirić A, Soković M, Barros L, Ferreira IC. Bioactive properties of greenhouse-cultivated green beans (Phaseolus vulgaris L.) under biostimulants and water-stress effect.. in Journal of the Science of Food and Agriculture. 2019;.
doi:10.1002/jsfa.9881 .

Petropoulos, Spyridon A, Taofiq, Oludemi, Fernandes, Ângela, Tzortzakis, Nikos, Ćirić, Ana, Soković, Marina, Barros, Lillian, Ferreira, Isabel CFR, "Bioactive properties of greenhouse-cultivated green beans (Phaseolus vulgaris L.) under biostimulants and water-stress effect." in Journal of the Science of Food and Agriculture (2019),
https://doi.org/10.1002/jsfa.9881 . .

TY  - JOUR
AU  - Pereira, Carla
AU  - Dias, Maria Inês
AU  - Petropoulos, Spyridon A.
AU  - Plexida, Sofia
AU  - Chrysargyris, Antonios
AU  - Tzortzakis, Nikos
AU  - Calhelha, Ricardo C.
AU  - Ivanov, Marija
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - C F R Ferreira, Isabel
PY  - 2019
UR  - https://www.mdpi.com/1420-3049/24/24/4494
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3577
AB  - In the present study, the effect of biostimulants application on the nutritional quality and bioactive properties of spinach cultivated in protected environment under water stress conditions was evaluated. For this purpose, four commercially available biostimulant products (Megafol (MEG), Aminovert (AM), Veramin Ca (V), Twin Antistress (TA), and two spinach genotypes (Fuji F1 and Viroflay) were tested under two irrigation regimes (normal irrigation (W+), and water-holding (W-). Fat and carbohydrates content was favored by water stress when Megafol (MEGW+) and Veramin (VW+) were applied on Fuji plants, while calorific value was also increased by MEGW+ treatment. In contrast, protein and ash content increased when AMW- and TAW+ were applied on Viroflay plants. Raffinose and glucose were the most abundant sugars, followed by sucrose and fructose, with the highest contents recorded for Fuji plants when AMW+ (fructose, glucose and total carbohydrates), CW- (sucrose), and TAW- (raffinose) treatments were applied. Regarding organic acids, oxalic and malic acid which had the highest contents for the TAW- (Viroflay plants) and AMW- (Fuji plants) treatments, respectively. α- and γ-tocopherol were the only isoforms detected with MEGW- and VW- inducing the biosynthesis of α-tocopherol, while AMW+ increased γ-tocopherol content in Fuji plants. The main fatty acids were α-linolenic and linoleic acids which were detected in the highest amounts in AMW-, AMW+, and TAW+ the former and in AMW-, VW-, and CW+ the latter. Regarding phenolic compounds content, peak 12 (5,3',4'-Trihydroxy-3-methoxy-6:7-methylenedioxyflavone-4'-glucuronide) was the most abundant compound, especially in Viroflay plants under normal irrigation and no biostimulants added (CW-). The antioxidant and cytotoxic activity of the tested samples did not show promising results when compared with the positive controls, while a variable antibacterial activity was recorded depending on the tested biostimulant, irrigation regime and genotype. In conclusion, a variable effect of the tested biostimulants and irrigation regimes was observed on bioactive properties and chemical composition of both spinach genotypes which highlights the need for further research in order to make profound conclusions regarding the positive effects of biostimulants under water stress conditions.
PB  - Multidisciplinary Digital Publishing Institute
T2  - Molecules (Basel, Switzerland)
T1  - The Effects of Biostimulants, Biofertilizers and Water-Stress on Nutritional Value and Chemical Composition of Two Spinach Genotypes (Spinacia oleracea L.).
IS  - 24
VL  - 24
DO  - 10.3390/molecules24244494
SP  - 4494
ER  - 

@article{
author = "Pereira, Carla and Dias, Maria Inês and Petropoulos, Spyridon A. and Plexida, Sofia and Chrysargyris, Antonios and Tzortzakis, Nikos and Calhelha, Ricardo C. and Ivanov, Marija and Stojković, Dejan and Soković, Marina and Barros, Lillian and C F R Ferreira, Isabel",
year = "2019",
abstract = "In the present study, the effect of biostimulants application on the nutritional quality and bioactive properties of spinach cultivated in protected environment under water stress conditions was evaluated. For this purpose, four commercially available biostimulant products (Megafol (MEG), Aminovert (AM), Veramin Ca (V), Twin Antistress (TA), and two spinach genotypes (Fuji F1 and Viroflay) were tested under two irrigation regimes (normal irrigation (W+), and water-holding (W-). Fat and carbohydrates content was favored by water stress when Megafol (MEGW+) and Veramin (VW+) were applied on Fuji plants, while calorific value was also increased by MEGW+ treatment. In contrast, protein and ash content increased when AMW- and TAW+ were applied on Viroflay plants. Raffinose and glucose were the most abundant sugars, followed by sucrose and fructose, with the highest contents recorded for Fuji plants when AMW+ (fructose, glucose and total carbohydrates), CW- (sucrose), and TAW- (raffinose) treatments were applied. Regarding organic acids, oxalic and malic acid which had the highest contents for the TAW- (Viroflay plants) and AMW- (Fuji plants) treatments, respectively. α- and γ-tocopherol were the only isoforms detected with MEGW- and VW- inducing the biosynthesis of α-tocopherol, while AMW+ increased γ-tocopherol content in Fuji plants. The main fatty acids were α-linolenic and linoleic acids which were detected in the highest amounts in AMW-, AMW+, and TAW+ the former and in AMW-, VW-, and CW+ the latter. Regarding phenolic compounds content, peak 12 (5,3',4'-Trihydroxy-3-methoxy-6:7-methylenedioxyflavone-4'-glucuronide) was the most abundant compound, especially in Viroflay plants under normal irrigation and no biostimulants added (CW-). The antioxidant and cytotoxic activity of the tested samples did not show promising results when compared with the positive controls, while a variable antibacterial activity was recorded depending on the tested biostimulant, irrigation regime and genotype. In conclusion, a variable effect of the tested biostimulants and irrigation regimes was observed on bioactive properties and chemical composition of both spinach genotypes which highlights the need for further research in order to make profound conclusions regarding the positive effects of biostimulants under water stress conditions.",
publisher = "Multidisciplinary Digital Publishing Institute",
journal = "Molecules (Basel, Switzerland)",
title = "The Effects of Biostimulants, Biofertilizers and Water-Stress on Nutritional Value and Chemical Composition of Two Spinach Genotypes (Spinacia oleracea L.).",
number = "24",
volume = "24",
doi = "10.3390/molecules24244494",
pages = "4494"
}

Pereira, C., Dias, M. I., Petropoulos, S. A., Plexida, S., Chrysargyris, A., Tzortzakis, N., Calhelha, R. C., Ivanov, M., Stojković, D., Soković, M., Barros, L.,& C F R Ferreira, I.. (2019). The Effects of Biostimulants, Biofertilizers and Water-Stress on Nutritional Value and Chemical Composition of Two Spinach Genotypes (Spinacia oleracea L.).. in Molecules (Basel, Switzerland)
Multidisciplinary Digital Publishing Institute., 24(24), 4494.
https://doi.org/10.3390/molecules24244494

Pereira C, Dias MI, Petropoulos SA, Plexida S, Chrysargyris A, Tzortzakis N, Calhelha RC, Ivanov M, Stojković D, Soković M, Barros L, C F R Ferreira I. The Effects of Biostimulants, Biofertilizers and Water-Stress on Nutritional Value and Chemical Composition of Two Spinach Genotypes (Spinacia oleracea L.).. in Molecules (Basel, Switzerland). 2019;24(24):4494.
doi:10.3390/molecules24244494 .

Pereira, Carla, Dias, Maria Inês, Petropoulos, Spyridon A., Plexida, Sofia, Chrysargyris, Antonios, Tzortzakis, Nikos, Calhelha, Ricardo C., Ivanov, Marija, Stojković, Dejan, Soković, Marina, Barros, Lillian, C F R Ferreira, Isabel, "The Effects of Biostimulants, Biofertilizers and Water-Stress on Nutritional Value and Chemical Composition of Two Spinach Genotypes (Spinacia oleracea L.)." in Molecules (Basel, Switzerland), 24, no. 24 (2019):4494,
https://doi.org/10.3390/molecules24244494 . .

TY  - JOUR
AU  - Petropoulos, Spyridon
AU  - Fernandes, Ângela
AU  - Stojković, Dejan
AU  - Pereira, Carla
AU  - Taofiq, Oludemi
AU  - Di Gioia, Francesco
AU  - Tzortzakis, Nikos
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Ferreira, Isabel C.F.R.
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0959652619331245?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3471
AB  - The intensification of horticultural crops cultivation makes urgent the seeking for alternative growth substrates that could substitute non-renewable and/or synthetic growing media, such as peat and rock wool. The aim of the present study was to evaluate the potential use of byproducts from two industrial crops commonly cultivated in the Mediterranean basin, namely cardoon and cotton, as growth substrates for Cichorium spinosum, while zeolite addition was also tested as a soil amendment. A pot experiment was carried for two consecutive growing periods and plant growth was evaluated for six growing media compositions, while plant extracts were also evaluated in terms of their phenolic compounds profile, antioxidant and antimicrobial activities. The results of this study showed that cotton byproducts and zeolite may partially substitute peat in growth substrate of C. spinosum and high yields comparable to peat may be achieved. Phenolic compounds content and antioxidant activity of leaves' extracts was higher for plants grown in soil which showed severe stress symptoms comparing to the other tested substrate blends. Antimicrobial activity was also affected by growth substrate composition, only in the case of antibacterial properties of leaves' extracts, whereas none of the extracts presented significant antifungal activities. In conclusion, the use of cotton ginning byproducts and zeolite in growth substrate blends may partially substitute conventional substrates as peat in horticultural crops production, resulting in reduction of production cost and lessening of bulky byproducts’ management and related environmental burden without compromising yield.
PB  - Elsevier
T2  - Journal of Cleaner Production
T1  - Cotton and cardoon byproducts as potential growing media components for Cichorium spinosum L. commercial cultivation
VL  - 240
DO  - 10.1016/J.JCLEPRO.2019.118254
SP  - 118254
ER  - 

@article{
author = "Petropoulos, Spyridon and Fernandes, Ângela and Stojković, Dejan and Pereira, Carla and Taofiq, Oludemi and Di Gioia, Francesco and Tzortzakis, Nikos and Soković, Marina and Barros, Lillian and Ferreira, Isabel C.F.R.",
year = "2019",
abstract = "The intensification of horticultural crops cultivation makes urgent the seeking for alternative growth substrates that could substitute non-renewable and/or synthetic growing media, such as peat and rock wool. The aim of the present study was to evaluate the potential use of byproducts from two industrial crops commonly cultivated in the Mediterranean basin, namely cardoon and cotton, as growth substrates for Cichorium spinosum, while zeolite addition was also tested as a soil amendment. A pot experiment was carried for two consecutive growing periods and plant growth was evaluated for six growing media compositions, while plant extracts were also evaluated in terms of their phenolic compounds profile, antioxidant and antimicrobial activities. The results of this study showed that cotton byproducts and zeolite may partially substitute peat in growth substrate of C. spinosum and high yields comparable to peat may be achieved. Phenolic compounds content and antioxidant activity of leaves' extracts was higher for plants grown in soil which showed severe stress symptoms comparing to the other tested substrate blends. Antimicrobial activity was also affected by growth substrate composition, only in the case of antibacterial properties of leaves' extracts, whereas none of the extracts presented significant antifungal activities. In conclusion, the use of cotton ginning byproducts and zeolite in growth substrate blends may partially substitute conventional substrates as peat in horticultural crops production, resulting in reduction of production cost and lessening of bulky byproducts’ management and related environmental burden without compromising yield.",
publisher = "Elsevier",
journal = "Journal of Cleaner Production",
title = "Cotton and cardoon byproducts as potential growing media components for Cichorium spinosum L. commercial cultivation",
volume = "240",
doi = "10.1016/J.JCLEPRO.2019.118254",
pages = "118254"
}

Petropoulos, S., Fernandes, Â., Stojković, D., Pereira, C., Taofiq, O., Di Gioia, F., Tzortzakis, N., Soković, M., Barros, L.,& Ferreira, I. C.F.R.. (2019). Cotton and cardoon byproducts as potential growing media components for Cichorium spinosum L. commercial cultivation. in Journal of Cleaner Production
Elsevier., 240, 118254.
https://doi.org/10.1016/J.JCLEPRO.2019.118254

Petropoulos S, Fernandes Â, Stojković D, Pereira C, Taofiq O, Di Gioia F, Tzortzakis N, Soković M, Barros L, Ferreira IC. Cotton and cardoon byproducts as potential growing media components for Cichorium spinosum L. commercial cultivation. in Journal of Cleaner Production. 2019;240:118254.
doi:10.1016/J.JCLEPRO.2019.118254 .

Petropoulos, Spyridon, Fernandes, Ângela, Stojković, Dejan, Pereira, Carla, Taofiq, Oludemi, Di Gioia, Francesco, Tzortzakis, Nikos, Soković, Marina, Barros, Lillian, Ferreira, Isabel C.F.R., "Cotton and cardoon byproducts as potential growing media components for Cichorium spinosum L. commercial cultivation" in Journal of Cleaner Production, 240 (2019):118254,
https://doi.org/10.1016/J.JCLEPRO.2019.118254 . .

TY  - JOUR
AU  - Petropoulos, Spyridon
AU  - Fernandes, Ângela
AU  - Pereira, Carla
AU  - Tzortzakis, Nikos
AU  - Vaz, Josiana
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Ferreira, Isabel C.F.R.
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0308814619305436?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3314
AB  - In the present study, the nutritional value, bioactive properties, and chemical composition of various cardoon (Cynara cardunculus L.) genotypes cultivated in central Greece were investigated. The results demonstrated that Cynara seeds are a good source of fat and protein, while they also contain considerable amounts of K, Mg, and Fe and low amount of Na. Sucrose, oxalic acid, and α-tocopherol were the only free sugar, organic acid, and tocopherol isoform respectively, found among the studied genotypes. The most abundant fatty acids were linoleic, oleic and palmitic acid, while PUFA was the most abundant fatty acid class. All the tested seeds contained only two phenolic compounds, namely 5-O-caffeoylquinic acid and 3,5-O-caffeoylquinic acid, while significant antioxidant activities and cytotoxicity against tumor cell lines and antimicrobial effects were also observed. In conclusion, cardoon seed extracts could be exploited in the food and pharmaceutical industries as alternative sources of natural compounds with bioactive properties.
T2  - Food Chemistry
T1  - Bioactivities, chemical composition and nutritional value of Cynara cardunculus L. seeds
VL  - 289
DO  - 10.1016/J.FOODCHEM.2019.03.066
SP  - 404
EP  - 412
ER  - 

@article{
author = "Petropoulos, Spyridon and Fernandes, Ângela and Pereira, Carla and Tzortzakis, Nikos and Vaz, Josiana and Soković, Marina and Barros, Lillian and Ferreira, Isabel C.F.R.",
year = "2019",
abstract = "In the present study, the nutritional value, bioactive properties, and chemical composition of various cardoon (Cynara cardunculus L.) genotypes cultivated in central Greece were investigated. The results demonstrated that Cynara seeds are a good source of fat and protein, while they also contain considerable amounts of K, Mg, and Fe and low amount of Na. Sucrose, oxalic acid, and α-tocopherol were the only free sugar, organic acid, and tocopherol isoform respectively, found among the studied genotypes. The most abundant fatty acids were linoleic, oleic and palmitic acid, while PUFA was the most abundant fatty acid class. All the tested seeds contained only two phenolic compounds, namely 5-O-caffeoylquinic acid and 3,5-O-caffeoylquinic acid, while significant antioxidant activities and cytotoxicity against tumor cell lines and antimicrobial effects were also observed. In conclusion, cardoon seed extracts could be exploited in the food and pharmaceutical industries as alternative sources of natural compounds with bioactive properties.",
journal = "Food Chemistry",
title = "Bioactivities, chemical composition and nutritional value of Cynara cardunculus L. seeds",
volume = "289",
doi = "10.1016/J.FOODCHEM.2019.03.066",
pages = "404-412"
}

Petropoulos, S., Fernandes, Â., Pereira, C., Tzortzakis, N., Vaz, J., Soković, M., Barros, L.,& Ferreira, I. C.F.R.. (2019). Bioactivities, chemical composition and nutritional value of Cynara cardunculus L. seeds. in Food Chemistry, 289, 404-412.
https://doi.org/10.1016/J.FOODCHEM.2019.03.066

Petropoulos S, Fernandes Â, Pereira C, Tzortzakis N, Vaz J, Soković M, Barros L, Ferreira IC. Bioactivities, chemical composition and nutritional value of Cynara cardunculus L. seeds. in Food Chemistry. 2019;289:404-412.
doi:10.1016/J.FOODCHEM.2019.03.066 .

Petropoulos, Spyridon, Fernandes, Ângela, Pereira, Carla, Tzortzakis, Nikos, Vaz, Josiana, Soković, Marina, Barros, Lillian, Ferreira, Isabel C.F.R., "Bioactivities, chemical composition and nutritional value of Cynara cardunculus L. seeds" in Food Chemistry, 289 (2019):404-412,
https://doi.org/10.1016/J.FOODCHEM.2019.03.066 . .