TY  - JOUR
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Petrović, Tamara
AU  - Radovanović, Tijana
AU  - Gavrić, Jelena
AU  - Mirč, Marko
AU  - Anđelković, Marko
AU  - Vukov, Tanja
AU  - Tomašević Kolarov, Nataša
AU  - Prokić, Marko
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6580
AB  - The effect of anesthesia/euthanasia with ethyl 3-aminobenzoate methanesulfonate (MS-222) on the oxidative status of Hyla arborea tadpoles was examined to determine whether the use of the anesthetic can confound the experimental results of the oxidative stress-based investigation. The experiment was conducted on two groups of tadpoles reared at different temperatures to produce differences in antioxidant capacity between the groups. After development at different temperatures (20 °C and 25 °C), the animals were exposed to different concentrations of MS-222 (0, 0.1, 1, and 5 g/L) for 15 min. The higher temperature decreased catalase activity, glutathione and protein carbonyl levels and increased glutathione reductase activity. The glutathione level and glutathione/thiol-related parameters were significantly changed after MS-222 exposure. However, individuals from the different temperature groups responded differently to the tested anesthetic, pointing to the possible influence of the initial levels of antioxidant capacity. The analysis of the interaction between the factors (temperature and MS-222) confirmed that the anesthetic can confound the results regarding the effects of temperature on the oxidative status parameters. The concentration of 0.1 g/L MS-222 had the lowest influence on the alterations in oxidative status and the results of the effect of temperature. A brief review of the current literature on the use of MS-222 in tadpoles made clear the absence of precise information on anesthetic concentration and exposure time. Similar studies should be repeated and extended to other amphibian species and other factors of interest to provide better guidance on tadpole anesthesia/euthanasia for future experiments that consider oxidative status parameters.
PB  - Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Does the anesthetic tricaine methanesulfonate (MS-222) distort oxidative status parameters in tadpoles?
VL  - 278
DO  - 10.1016/j.cbpc.2024.109859
SP  - 109859
ER  - 

@article{
author = "Gavrilović, Branka and Despotović, Svetlana and Petrović, Tamara and Radovanović, Tijana and Gavrić, Jelena and Mirč, Marko and Anđelković, Marko and Vukov, Tanja and Tomašević Kolarov, Nataša and Prokić, Marko",
year = "2024",
abstract = "The effect of anesthesia/euthanasia with ethyl 3-aminobenzoate methanesulfonate (MS-222) on the oxidative status of Hyla arborea tadpoles was examined to determine whether the use of the anesthetic can confound the experimental results of the oxidative stress-based investigation. The experiment was conducted on two groups of tadpoles reared at different temperatures to produce differences in antioxidant capacity between the groups. After development at different temperatures (20 °C and 25 °C), the animals were exposed to different concentrations of MS-222 (0, 0.1, 1, and 5 g/L) for 15 min. The higher temperature decreased catalase activity, glutathione and protein carbonyl levels and increased glutathione reductase activity. The glutathione level and glutathione/thiol-related parameters were significantly changed after MS-222 exposure. However, individuals from the different temperature groups responded differently to the tested anesthetic, pointing to the possible influence of the initial levels of antioxidant capacity. The analysis of the interaction between the factors (temperature and MS-222) confirmed that the anesthetic can confound the results regarding the effects of temperature on the oxidative status parameters. The concentration of 0.1 g/L MS-222 had the lowest influence on the alterations in oxidative status and the results of the effect of temperature. A brief review of the current literature on the use of MS-222 in tadpoles made clear the absence of precise information on anesthetic concentration and exposure time. Similar studies should be repeated and extended to other amphibian species and other factors of interest to provide better guidance on tadpole anesthesia/euthanasia for future experiments that consider oxidative status parameters.",
publisher = "Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Does the anesthetic tricaine methanesulfonate (MS-222) distort oxidative status parameters in tadpoles?",
volume = "278",
doi = "10.1016/j.cbpc.2024.109859",
pages = "109859"
}

Gavrilović, B., Despotović, S., Petrović, T., Radovanović, T., Gavrić, J., Mirč, M., Anđelković, M., Vukov, T., Tomašević Kolarov, N.,& Prokić, M.. (2024). Does the anesthetic tricaine methanesulfonate (MS-222) distort oxidative status parameters in tadpoles?. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
Elsevier Inc.., 278, 109859.
https://doi.org/10.1016/j.cbpc.2024.109859

Gavrilović B, Despotović S, Petrović T, Radovanović T, Gavrić J, Mirč M, Anđelković M, Vukov T, Tomašević Kolarov N, Prokić M. Does the anesthetic tricaine methanesulfonate (MS-222) distort oxidative status parameters in tadpoles?. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2024;278:109859.
doi:10.1016/j.cbpc.2024.109859 .

Gavrilović, Branka, Despotović, Svetlana, Petrović, Tamara, Radovanović, Tijana, Gavrić, Jelena, Mirč, Marko, Anđelković, Marko, Vukov, Tanja, Tomašević Kolarov, Nataša, Prokić, Marko, "Does the anesthetic tricaine methanesulfonate (MS-222) distort oxidative status parameters in tadpoles?" in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 278 (2024):109859,
https://doi.org/10.1016/j.cbpc.2024.109859 . .

TY  - JOUR
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Petrović, Tamara
AU  - Radovanović, Tijana
AU  - Gavrić, Jelena
AU  - Mirč, Marko
AU  - Anđelković, Marko
AU  - Vukov, Tanja
AU  - Tomašević Kolarov, Nataša
AU  - Prokić, Marko
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6579
AB  - The effect of anesthesia/euthanasia with ethyl 3-aminobenzoate methanesulfonate (MS-222) on the oxidative status of Hyla arborea tadpoles was examined to determine whether the use of the anesthetic can confound the experimental results of the oxidative stress-based investigation. The experiment was conducted on two groups of tadpoles reared at different temperatures to produce differences in antioxidant capacity between the groups. After development at different temperatures (20 °C and 25 °C), the animals were exposed to different concentrations of MS-222 (0, 0.1, 1, and 5 g/L) for 15 min. The higher temperature decreased catalase activity, glutathione and protein carbonyl levels and increased glutathione reductase activity. The glutathione level and glutathione/thiol-related parameters were significantly changed after MS-222 exposure. However, individuals from the different temperature groups responded differently to the tested anesthetic, pointing to the possible influence of the initial levels of antioxidant capacity. The analysis of the interaction between the factors (temperature and MS-222) confirmed that the anesthetic can confound the results regarding the effects of temperature on the oxidative status parameters. The concentration of 0.1 g/L MS-222 had the lowest influence on the alterations in oxidative status and the results of the effect of temperature. A brief review of the current literature on the use of MS-222 in tadpoles made clear the absence of precise information on anesthetic concentration and exposure time. Similar studies should be repeated and extended to other amphibian species and other factors of interest to provide better guidance on tadpole anesthesia/euthanasia for future experiments that consider oxidative status parameters.
PB  - Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Does the anesthetic tricaine methanesulfonate (MS-222) distort oxidative status parameters in tadpoles?
VL  - 278
DO  - 10.1016/j.cbpc.2024.109859
SP  - 109859
ER  - 

@article{
author = "Gavrilović, Branka and Despotović, Svetlana and Petrović, Tamara and Radovanović, Tijana and Gavrić, Jelena and Mirč, Marko and Anđelković, Marko and Vukov, Tanja and Tomašević Kolarov, Nataša and Prokić, Marko",
year = "2024",
abstract = "The effect of anesthesia/euthanasia with ethyl 3-aminobenzoate methanesulfonate (MS-222) on the oxidative status of Hyla arborea tadpoles was examined to determine whether the use of the anesthetic can confound the experimental results of the oxidative stress-based investigation. The experiment was conducted on two groups of tadpoles reared at different temperatures to produce differences in antioxidant capacity between the groups. After development at different temperatures (20 °C and 25 °C), the animals were exposed to different concentrations of MS-222 (0, 0.1, 1, and 5 g/L) for 15 min. The higher temperature decreased catalase activity, glutathione and protein carbonyl levels and increased glutathione reductase activity. The glutathione level and glutathione/thiol-related parameters were significantly changed after MS-222 exposure. However, individuals from the different temperature groups responded differently to the tested anesthetic, pointing to the possible influence of the initial levels of antioxidant capacity. The analysis of the interaction between the factors (temperature and MS-222) confirmed that the anesthetic can confound the results regarding the effects of temperature on the oxidative status parameters. The concentration of 0.1 g/L MS-222 had the lowest influence on the alterations in oxidative status and the results of the effect of temperature. A brief review of the current literature on the use of MS-222 in tadpoles made clear the absence of precise information on anesthetic concentration and exposure time. Similar studies should be repeated and extended to other amphibian species and other factors of interest to provide better guidance on tadpole anesthesia/euthanasia for future experiments that consider oxidative status parameters.",
publisher = "Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Does the anesthetic tricaine methanesulfonate (MS-222) distort oxidative status parameters in tadpoles?",
volume = "278",
doi = "10.1016/j.cbpc.2024.109859",
pages = "109859"
}

Gavrilović, B., Despotović, S., Petrović, T., Radovanović, T., Gavrić, J., Mirč, M., Anđelković, M., Vukov, T., Tomašević Kolarov, N.,& Prokić, M.. (2024). Does the anesthetic tricaine methanesulfonate (MS-222) distort oxidative status parameters in tadpoles?. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
Elsevier Inc.., 278, 109859.
https://doi.org/10.1016/j.cbpc.2024.109859

Gavrilović B, Despotović S, Petrović T, Radovanović T, Gavrić J, Mirč M, Anđelković M, Vukov T, Tomašević Kolarov N, Prokić M. Does the anesthetic tricaine methanesulfonate (MS-222) distort oxidative status parameters in tadpoles?. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2024;278:109859.
doi:10.1016/j.cbpc.2024.109859 .

Gavrilović, Branka, Despotović, Svetlana, Petrović, Tamara, Radovanović, Tijana, Gavrić, Jelena, Mirč, Marko, Anđelković, Marko, Vukov, Tanja, Tomašević Kolarov, Nataša, Prokić, Marko, "Does the anesthetic tricaine methanesulfonate (MS-222) distort oxidative status parameters in tadpoles?" in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 278 (2024):109859,
https://doi.org/10.1016/j.cbpc.2024.109859 . .

TY  - JOUR
AU  - Morshedi, Vahid
AU  - Noori, Farzaneh
AU  - Prokić, Marko
AU  - Petrović, Tamara
AU  - Jafari, Fateme
AU  - Moradian, Seyed Hossein
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6551
AB  - Dynamic changes of tissues, organs and growth that occur in fish larvae during the transition to the juvenile stage are accompanied by differences in metabolic, locomotor and feeding activities that can reflect on fish's oxidative status. In this study, we examine how body growth, antioxidant system (superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) and total antioxidant capacity (TAC) and oxidative damage (malondialdehyde-MDA) parameters change in larvae of yellowfin seabream larvae (Acanthopagrus latus) during early development (0, 7, 15, 22 and 30 day after hatching-DAH). Body growth (length and weight) starts to intensify from 15 DAH. We observed general increase in the antioxidant system (AOS) with the age of larvae from newly hatched and 7 DAH up to 15 and 22 DAH individuals. 15 and 22 DAH larvae had the greatest levels of TAC, SOD and GR activity, while 30 DAH larvae had higher CAT activity from 0, 7 and 15 DAH and MDA concentration in comparison to 15 DAH individuals. Several developmental events can be linked with observed results: lower AOS in 0 and 7 DAH individuals with low locomotor activity, growth, endo-exogenous feeding phase and cell differentiation; 15 and 22 DAH larvae are under pressure of fast growth, enhanced swimming and foraging capacity; while higher MDA production in 30 DAH larvae can be a result of shifts in muscle metabolism, changes in both quality and quantity of food and a significant increase in weight. The present study provides insight into the changes in redox status during the ontogeny of A. latus, fish species about which physiology is still little known but with a potential for use in marine culture. Ability to lower oxidative stress during critical developmental periods can enhance that potential.
PB  - Amsterdam, Netherlands: Elsevier
T2  - Comparative Biochemistry and Physiology, Part A: Molecular and Integrative Physiology
T1  - Changes of oxidative status in yellowfin seabream larvae (Acanthopagrus latus) during development
VL  - 281
DO  - 10.1016/j.cbpa.2023.111433
SP  - 111433
ER  - 

@article{
author = "Morshedi, Vahid and Noori, Farzaneh and Prokić, Marko and Petrović, Tamara and Jafari, Fateme and Moradian, Seyed Hossein",
year = "2023",
abstract = "Dynamic changes of tissues, organs and growth that occur in fish larvae during the transition to the juvenile stage are accompanied by differences in metabolic, locomotor and feeding activities that can reflect on fish's oxidative status. In this study, we examine how body growth, antioxidant system (superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) and total antioxidant capacity (TAC) and oxidative damage (malondialdehyde-MDA) parameters change in larvae of yellowfin seabream larvae (Acanthopagrus latus) during early development (0, 7, 15, 22 and 30 day after hatching-DAH). Body growth (length and weight) starts to intensify from 15 DAH. We observed general increase in the antioxidant system (AOS) with the age of larvae from newly hatched and 7 DAH up to 15 and 22 DAH individuals. 15 and 22 DAH larvae had the greatest levels of TAC, SOD and GR activity, while 30 DAH larvae had higher CAT activity from 0, 7 and 15 DAH and MDA concentration in comparison to 15 DAH individuals. Several developmental events can be linked with observed results: lower AOS in 0 and 7 DAH individuals with low locomotor activity, growth, endo-exogenous feeding phase and cell differentiation; 15 and 22 DAH larvae are under pressure of fast growth, enhanced swimming and foraging capacity; while higher MDA production in 30 DAH larvae can be a result of shifts in muscle metabolism, changes in both quality and quantity of food and a significant increase in weight. The present study provides insight into the changes in redox status during the ontogeny of A. latus, fish species about which physiology is still little known but with a potential for use in marine culture. Ability to lower oxidative stress during critical developmental periods can enhance that potential.",
publisher = "Amsterdam, Netherlands: Elsevier",
journal = "Comparative Biochemistry and Physiology, Part A: Molecular and Integrative Physiology",
title = "Changes of oxidative status in yellowfin seabream larvae (Acanthopagrus latus) during development",
volume = "281",
doi = "10.1016/j.cbpa.2023.111433",
pages = "111433"
}

Morshedi, V., Noori, F., Prokić, M., Petrović, T., Jafari, F.,& Moradian, S. H.. (2023). Changes of oxidative status in yellowfin seabream larvae (Acanthopagrus latus) during development. in Comparative Biochemistry and Physiology, Part A: Molecular and Integrative Physiology
Amsterdam, Netherlands: Elsevier., 281, 111433.
https://doi.org/10.1016/j.cbpa.2023.111433

Morshedi V, Noori F, Prokić M, Petrović T, Jafari F, Moradian SH. Changes of oxidative status in yellowfin seabream larvae (Acanthopagrus latus) during development. in Comparative Biochemistry and Physiology, Part A: Molecular and Integrative Physiology. 2023;281:111433.
doi:10.1016/j.cbpa.2023.111433 .

Morshedi, Vahid, Noori, Farzaneh, Prokić, Marko, Petrović, Tamara, Jafari, Fateme, Moradian, Seyed Hossein, "Changes of oxidative status in yellowfin seabream larvae (Acanthopagrus latus) during development" in Comparative Biochemistry and Physiology, Part A: Molecular and Integrative Physiology, 281 (2023):111433,
https://doi.org/10.1016/j.cbpa.2023.111433 . .

TY  - JOUR
AU  - Morshedi, Vahid
AU  - Noori, Farzaneh
AU  - Prokić, Marko
AU  - Petrović, Tamara
AU  - Jafari, Fateme
AU  - Moradian, Seyed Hossein
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6549
AB  - Dynamic changes of tissues, organs and growth that occur in fish larvae during the transition to the juvenile stage are accompanied by differences in metabolic, locomotor and feeding activities that can reflect on fish's oxidative status. In this study, we examine how body growth, antioxidant system (superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) and total antioxidant capacity (TAC) and oxidative damage (malondialdehyde-MDA) parameters change in larvae of yellowfin seabream larvae (Acanthopagrus latus) during early development (0, 7, 15, 22 and 30 day after hatching-DAH). Body growth (length and weight) starts to intensify from 15 DAH. We observed general increase in the antioxidant system (AOS) with the age of larvae from newly hatched and 7 DAH up to 15 and 22 DAH individuals. 15 and 22 DAH larvae had the greatest levels of TAC, SOD and GR activity, while 30 DAH larvae had higher CAT activity from 0, 7 and 15 DAH and MDA concentration in comparison to 15 DAH individuals. Several developmental events can be linked with observed results: lower AOS in 0 and 7 DAH individuals with low locomotor activity, growth, endo-exogenous feeding phase and cell differentiation; 15 and 22 DAH larvae are under pressure of fast growth, enhanced swimming and foraging capacity; while higher MDA production in 30 DAH larvae can be a result of shifts in muscle metabolism, changes in both quality and quantity of food and a significant increase in weight. The present study provides insight into the changes in redox status during the ontogeny of A. latus, fish species about which physiology is still little known but with a potential for use in marine culture. Ability to lower oxidative stress during critical developmental periods can enhance that potential.
PB  - Amsterdam, Netherlands: Elsevier
T2  - Comparative Biochemistry and Physiology, Part A: Molecular and Integrative Physiology
T1  - Changes of oxidative status in yellowfin seabream larvae (Acanthopagrus latus) during development
VL  - 281
DO  - 10.1016/j.cbpa.2023.111433
SP  - 111433
ER  - 

@article{
author = "Morshedi, Vahid and Noori, Farzaneh and Prokić, Marko and Petrović, Tamara and Jafari, Fateme and Moradian, Seyed Hossein",
year = "2023",
abstract = "Dynamic changes of tissues, organs and growth that occur in fish larvae during the transition to the juvenile stage are accompanied by differences in metabolic, locomotor and feeding activities that can reflect on fish's oxidative status. In this study, we examine how body growth, antioxidant system (superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) and total antioxidant capacity (TAC) and oxidative damage (malondialdehyde-MDA) parameters change in larvae of yellowfin seabream larvae (Acanthopagrus latus) during early development (0, 7, 15, 22 and 30 day after hatching-DAH). Body growth (length and weight) starts to intensify from 15 DAH. We observed general increase in the antioxidant system (AOS) with the age of larvae from newly hatched and 7 DAH up to 15 and 22 DAH individuals. 15 and 22 DAH larvae had the greatest levels of TAC, SOD and GR activity, while 30 DAH larvae had higher CAT activity from 0, 7 and 15 DAH and MDA concentration in comparison to 15 DAH individuals. Several developmental events can be linked with observed results: lower AOS in 0 and 7 DAH individuals with low locomotor activity, growth, endo-exogenous feeding phase and cell differentiation; 15 and 22 DAH larvae are under pressure of fast growth, enhanced swimming and foraging capacity; while higher MDA production in 30 DAH larvae can be a result of shifts in muscle metabolism, changes in both quality and quantity of food and a significant increase in weight. The present study provides insight into the changes in redox status during the ontogeny of A. latus, fish species about which physiology is still little known but with a potential for use in marine culture. Ability to lower oxidative stress during critical developmental periods can enhance that potential.",
publisher = "Amsterdam, Netherlands: Elsevier",
journal = "Comparative Biochemistry and Physiology, Part A: Molecular and Integrative Physiology",
title = "Changes of oxidative status in yellowfin seabream larvae (Acanthopagrus latus) during development",
volume = "281",
doi = "10.1016/j.cbpa.2023.111433",
pages = "111433"
}

Morshedi, V., Noori, F., Prokić, M., Petrović, T., Jafari, F.,& Moradian, S. H.. (2023). Changes of oxidative status in yellowfin seabream larvae (Acanthopagrus latus) during development. in Comparative Biochemistry and Physiology, Part A: Molecular and Integrative Physiology
Amsterdam, Netherlands: Elsevier., 281, 111433.
https://doi.org/10.1016/j.cbpa.2023.111433

Morshedi V, Noori F, Prokić M, Petrović T, Jafari F, Moradian SH. Changes of oxidative status in yellowfin seabream larvae (Acanthopagrus latus) during development. in Comparative Biochemistry and Physiology, Part A: Molecular and Integrative Physiology. 2023;281:111433.
doi:10.1016/j.cbpa.2023.111433 .

Morshedi, Vahid, Noori, Farzaneh, Prokić, Marko, Petrović, Tamara, Jafari, Fateme, Moradian, Seyed Hossein, "Changes of oxidative status in yellowfin seabream larvae (Acanthopagrus latus) during development" in Comparative Biochemistry and Physiology, Part A: Molecular and Integrative Physiology, 281 (2023):111433,
https://doi.org/10.1016/j.cbpa.2023.111433 . .

TY  - CONF
AU  - Kijanović, Ana
AU  - Vukov, Tanja
AU  - Mirč, Marko
AU  - Mitrović, Aleksandar
AU  - Prokić, Marko
AU  - Petrović, Tamara
AU  - Radovanović, Tijana
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Tomašević Kolarov, Nataša
PY  - 2023
UR  - https://www.sebiology.org/events/seb-centenary-conference-2023/abstracts.html
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6060
AB  - Amphibian species that inhabit temporary ponds for reproduction maximize larval growth under favorable conditions and accelerate their development to undergo rapid metamorphosis under stressful conditions such as pond drying. Corticosterone controls development, metabolism, and growth, and has an invaluable role in anuran metamorphosis under stress conditions. In this study, we evaluated whether the whole-body corticosterone (CORT) level is related to drying conditions in species that cannot accelerate the developmental rate in response to pond drying. Specifically, we investigated the effects of different water levels in combination with exogenous CORT and corticosteroid synthesis inhibitor metyrapone (treatments: high water level, high water level with exogenous CORT, low water level, low water level with metyrapone) on the whole-body corticosterone (at prometamorphosis and metamorphic climax), life history and morphological traits at the metamorphic climax. We found that these conditions did not alter the whole-body content of CORT and the developmental rate in treatments, although low water levels and exogenous CORT in high water level negatively affected other life history traits and tail shape. Individuals from a high water level with exogenous CORT had the smallest body size and mass and changed tail shape at metamorphosis, while changes in life history traits did not affect the tail shape in the other treatments. Our findings indicate that the absence of developmental response (i.e. canalized development) in the timing of metamorphosis of B. variegata may be explained by a modification of endocrine regulation but further studies that would include closely related species are required.
PB  - Society for Experimental Biology
C3  - Abstract book: SEB Centenary Conference 1923-2023: Celebrating Success and Shaping the Future; 2023 Jul 4-7; Edinburgh, UK
T1  - Pond drying conditions do not alter whole-body corticosterone content and metamorphic time of yellow-bellied toad (Bombina variegata) metamorphs
SP  - 8
EP  - 9
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6060
ER  - 

@conference{
author = "Kijanović, Ana and Vukov, Tanja and Mirč, Marko and Mitrović, Aleksandar and Prokić, Marko and Petrović, Tamara and Radovanović, Tijana and Gavrilović, Branka and Despotović, Svetlana and Gavrić, Jelena and Tomašević Kolarov, Nataša",
year = "2023",
abstract = "Amphibian species that inhabit temporary ponds for reproduction maximize larval growth under favorable conditions and accelerate their development to undergo rapid metamorphosis under stressful conditions such as pond drying. Corticosterone controls development, metabolism, and growth, and has an invaluable role in anuran metamorphosis under stress conditions. In this study, we evaluated whether the whole-body corticosterone (CORT) level is related to drying conditions in species that cannot accelerate the developmental rate in response to pond drying. Specifically, we investigated the effects of different water levels in combination with exogenous CORT and corticosteroid synthesis inhibitor metyrapone (treatments: high water level, high water level with exogenous CORT, low water level, low water level with metyrapone) on the whole-body corticosterone (at prometamorphosis and metamorphic climax), life history and morphological traits at the metamorphic climax. We found that these conditions did not alter the whole-body content of CORT and the developmental rate in treatments, although low water levels and exogenous CORT in high water level negatively affected other life history traits and tail shape. Individuals from a high water level with exogenous CORT had the smallest body size and mass and changed tail shape at metamorphosis, while changes in life history traits did not affect the tail shape in the other treatments. Our findings indicate that the absence of developmental response (i.e. canalized development) in the timing of metamorphosis of B. variegata may be explained by a modification of endocrine regulation but further studies that would include closely related species are required.",
publisher = "Society for Experimental Biology",
journal = "Abstract book: SEB Centenary Conference 1923-2023: Celebrating Success and Shaping the Future; 2023 Jul 4-7; Edinburgh, UK",
title = "Pond drying conditions do not alter whole-body corticosterone content and metamorphic time of yellow-bellied toad (Bombina variegata) metamorphs",
pages = "8-9",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6060"
}

Kijanović, A., Vukov, T., Mirč, M., Mitrović, A., Prokić, M., Petrović, T., Radovanović, T., Gavrilović, B., Despotović, S., Gavrić, J.,& Tomašević Kolarov, N.. (2023). Pond drying conditions do not alter whole-body corticosterone content and metamorphic time of yellow-bellied toad (Bombina variegata) metamorphs. in Abstract book: SEB Centenary Conference 1923-2023: Celebrating Success and Shaping the Future; 2023 Jul 4-7; Edinburgh, UK
Society for Experimental Biology., 8-9.
https://hdl.handle.net/21.15107/rcub_ibiss_6060

Kijanović A, Vukov T, Mirč M, Mitrović A, Prokić M, Petrović T, Radovanović T, Gavrilović B, Despotović S, Gavrić J, Tomašević Kolarov N. Pond drying conditions do not alter whole-body corticosterone content and metamorphic time of yellow-bellied toad (Bombina variegata) metamorphs. in Abstract book: SEB Centenary Conference 1923-2023: Celebrating Success and Shaping the Future; 2023 Jul 4-7; Edinburgh, UK. 2023;:8-9.
https://hdl.handle.net/21.15107/rcub_ibiss_6060 .

Kijanović, Ana, Vukov, Tanja, Mirč, Marko, Mitrović, Aleksandar, Prokić, Marko, Petrović, Tamara, Radovanović, Tijana, Gavrilović, Branka, Despotović, Svetlana, Gavrić, Jelena, Tomašević Kolarov, Nataša, "Pond drying conditions do not alter whole-body corticosterone content and metamorphic time of yellow-bellied toad (Bombina variegata) metamorphs" in Abstract book: SEB Centenary Conference 1923-2023: Celebrating Success and Shaping the Future; 2023 Jul 4-7; Edinburgh, UK (2023):8-9,
https://hdl.handle.net/21.15107/rcub_ibiss_6060 .

TY  - JOUR
AU  - Petrović, Tamara
AU  - Vučić, Tijana
AU  - Burraco, Pablo
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Radovanović, Tijana
AU  - Šajkunić, Sanja
AU  - Ivanović, Ana
AU  - Prokić, Marko
PY  - 2023
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0306456523000153
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5463
AB  - Ectotherms are particularly sensitive to global warming due to their limited capacity to thermoregulate, which can impact their performance and fitness. From a physiological standpoint, higher temperatures often enhance biological processes that can induce the production of reactive oxygen species and result in a state of cellular oxidative stress. Temperature alters interspecific interactions, including species hybridization. Hybridization under different thermal conditions could amplify parental (genetic) incompatibilities, thus affecting a hybrid's development and distribution. Understanding the impact of global warming on the physiology of hybrids and particularly their oxidative status could help in predicting future scenarios in ecosystems and in hybrids. In the present study, we investigated the effect of water temperature on the development, growth and oxidative stress of two crested newt species and their reciprocal hybrids. Larvae of Triturus macedonicus and T. ivanbureschi, and their T. macedonicus-mothered and T. ivanbureschi-mothered hybrids were exposed for 30 days to temperatures of 19°C and 24°C. Under the higher temperature, the hybrids experienced increases in both growth and developmental rates, while parental species exhibited accelerated growth (T. macedonicus) or development (T. ivanbureschi). Warm conditions also had different effects on the oxidative status of hybrid and parental species. Parental species had enhanced antioxidant responses (catalase, glutathione peroxidase, glutathione S-transferase and SH groups), which allowed them to alleviate temperature-induced stress (revealed by the absence of oxidative damage). However, warming induced an antioxidant response in the hybrids, including oxidative damage in the form of lipid peroxidation. These findings point to a greater disruption of redox regulation and metabolic machinery in hybrid newts, which can be interpreted as the cost of hybridization that is likely linked to parental incompatibilities expressed under a higher temperature. Our study aims to improve mechanistic understanding of the resilience and distribution of hybrid species that cope with climate-driven changes.
PB  - Elsevier Ltd
T2  - Journal of Thermal Biology
T1  - Higher temperature induces oxidative stress in hybrids but not in parental species: A case study of crested newts
VL  - 112
DO  - 10.1016/j.jtherbio.2023.103474
SP  - 103474
ER  - 

@article{
author = "Petrović, Tamara and Vučić, Tijana and Burraco, Pablo and Gavrilović, Branka and Despotović, Svetlana and Gavrić, Jelena and Radovanović, Tijana and Šajkunić, Sanja and Ivanović, Ana and Prokić, Marko",
year = "2023",
abstract = "Ectotherms are particularly sensitive to global warming due to their limited capacity to thermoregulate, which can impact their performance and fitness. From a physiological standpoint, higher temperatures often enhance biological processes that can induce the production of reactive oxygen species and result in a state of cellular oxidative stress. Temperature alters interspecific interactions, including species hybridization. Hybridization under different thermal conditions could amplify parental (genetic) incompatibilities, thus affecting a hybrid's development and distribution. Understanding the impact of global warming on the physiology of hybrids and particularly their oxidative status could help in predicting future scenarios in ecosystems and in hybrids. In the present study, we investigated the effect of water temperature on the development, growth and oxidative stress of two crested newt species and their reciprocal hybrids. Larvae of Triturus macedonicus and T. ivanbureschi, and their T. macedonicus-mothered and T. ivanbureschi-mothered hybrids were exposed for 30 days to temperatures of 19°C and 24°C. Under the higher temperature, the hybrids experienced increases in both growth and developmental rates, while parental species exhibited accelerated growth (T. macedonicus) or development (T. ivanbureschi). Warm conditions also had different effects on the oxidative status of hybrid and parental species. Parental species had enhanced antioxidant responses (catalase, glutathione peroxidase, glutathione S-transferase and SH groups), which allowed them to alleviate temperature-induced stress (revealed by the absence of oxidative damage). However, warming induced an antioxidant response in the hybrids, including oxidative damage in the form of lipid peroxidation. These findings point to a greater disruption of redox regulation and metabolic machinery in hybrid newts, which can be interpreted as the cost of hybridization that is likely linked to parental incompatibilities expressed under a higher temperature. Our study aims to improve mechanistic understanding of the resilience and distribution of hybrid species that cope with climate-driven changes.",
publisher = "Elsevier Ltd",
journal = "Journal of Thermal Biology",
title = "Higher temperature induces oxidative stress in hybrids but not in parental species: A case study of crested newts",
volume = "112",
doi = "10.1016/j.jtherbio.2023.103474",
pages = "103474"
}

Petrović, T., Vučić, T., Burraco, P., Gavrilović, B., Despotović, S., Gavrić, J., Radovanović, T., Šajkunić, S., Ivanović, A.,& Prokić, M.. (2023). Higher temperature induces oxidative stress in hybrids but not in parental species: A case study of crested newts. in Journal of Thermal Biology
Elsevier Ltd., 112, 103474.
https://doi.org/10.1016/j.jtherbio.2023.103474

Petrović T, Vučić T, Burraco P, Gavrilović B, Despotović S, Gavrić J, Radovanović T, Šajkunić S, Ivanović A, Prokić M. Higher temperature induces oxidative stress in hybrids but not in parental species: A case study of crested newts. in Journal of Thermal Biology. 2023;112:103474.
doi:10.1016/j.jtherbio.2023.103474 .

Petrović, Tamara, Vučić, Tijana, Burraco, Pablo, Gavrilović, Branka, Despotović, Svetlana, Gavrić, Jelena, Radovanović, Tijana, Šajkunić, Sanja, Ivanović, Ana, Prokić, Marko, "Higher temperature induces oxidative stress in hybrids but not in parental species: A case study of crested newts" in Journal of Thermal Biology, 112 (2023):103474,
https://doi.org/10.1016/j.jtherbio.2023.103474 . .

TY  - JOUR
AU  - Radovanović, Tijana
AU  - Petrović, Tamara
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Kijanović, Ana
AU  - Mirč, Marko
AU  - Tomašević Kolarov, Nataša
AU  - Vukov, Tanja
AU  - Prokić, Marko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5464
AB  - Background Urban development results in habitat destruction, affecting populations of amphibians, the most fragile
group of vertebrates. With changes in the environment, these animals become more exposed to light and predators.
To enhance their chances of survival, they display plasticity of body coloration. Aside from adaptive benefits,
animals exhibiting background matching meet the energetic costs and restrictions of changing body tones. To study
the physiological consequences of Hyla arborea tadpole adaptation to background color, we followed oxidative stress
parameters after rearing larvae on a constant background (black/white) and after changing the background color.
Results Larvae cultivated for 20 days on constant substrate color exhibited differences in body coloration but without
differences in lipid peroxidation (LPO) concentration between dark and pale individuals, suggesting that coloration
investment during this period did not induce higher oxidative damage in darker tadpoles. Prolonged exposure
of larvae (37 days) to a dark habitat increased antioxidative system defense and LPO concentrations, compared to
animals reared permanently in the white surroundings. The positive correlation of oxidative damage with color
intensity of individuals points to the physiological consequences of higher investment in the number of pigment cells
necessary for dark pigmentation. In individuals faced with non-matching background and change in body coloration,
defense system declined and LPO occurred relative to individuals cultivated in white habitat.
Conclusion Here, we have pointed to consequences related to background matching and stress that amphibians
experienced during chromatic adaptations. Background color change causes a complex physiological response
affecting the antioxidative defense parameters. This investigation elucidates the accompanying cost of amphibiansʼ
adjustment to an altered environment.
PB  - London: BMC
T2  - Frontiers in Zoology
T1  - What coloration brings: Implications of background adaptation to oxidative stress in anurans
IS  - 1
VL  - 20
DO  - 10.1186/s12983-023-00486-z
SP  - 6
ER  - 

@article{
author = "Radovanović, Tijana and Petrović, Tamara and Gavrilović, Branka and Despotović, Svetlana and Gavrić, Jelena and Kijanović, Ana and Mirč, Marko and Tomašević Kolarov, Nataša and Vukov, Tanja and Prokić, Marko",
year = "2023",
abstract = "Background Urban development results in habitat destruction, affecting populations of amphibians, the most fragile
group of vertebrates. With changes in the environment, these animals become more exposed to light and predators.
To enhance their chances of survival, they display plasticity of body coloration. Aside from adaptive benefits,
animals exhibiting background matching meet the energetic costs and restrictions of changing body tones. To study
the physiological consequences of Hyla arborea tadpole adaptation to background color, we followed oxidative stress
parameters after rearing larvae on a constant background (black/white) and after changing the background color.
Results Larvae cultivated for 20 days on constant substrate color exhibited differences in body coloration but without
differences in lipid peroxidation (LPO) concentration between dark and pale individuals, suggesting that coloration
investment during this period did not induce higher oxidative damage in darker tadpoles. Prolonged exposure
of larvae (37 days) to a dark habitat increased antioxidative system defense and LPO concentrations, compared to
animals reared permanently in the white surroundings. The positive correlation of oxidative damage with color
intensity of individuals points to the physiological consequences of higher investment in the number of pigment cells
necessary for dark pigmentation. In individuals faced with non-matching background and change in body coloration,
defense system declined and LPO occurred relative to individuals cultivated in white habitat.
Conclusion Here, we have pointed to consequences related to background matching and stress that amphibians
experienced during chromatic adaptations. Background color change causes a complex physiological response
affecting the antioxidative defense parameters. This investigation elucidates the accompanying cost of amphibiansʼ
adjustment to an altered environment.",
publisher = "London: BMC",
journal = "Frontiers in Zoology",
title = "What coloration brings: Implications of background adaptation to oxidative stress in anurans",
number = "1",
volume = "20",
doi = "10.1186/s12983-023-00486-z",
pages = "6"
}

Radovanović, T., Petrović, T., Gavrilović, B., Despotović, S., Gavrić, J., Kijanović, A., Mirč, M., Tomašević Kolarov, N., Vukov, T.,& Prokić, M.. (2023). What coloration brings: Implications of background adaptation to oxidative stress in anurans. in Frontiers in Zoology
London: BMC., 20(1), 6.
https://doi.org/10.1186/s12983-023-00486-z

Radovanović T, Petrović T, Gavrilović B, Despotović S, Gavrić J, Kijanović A, Mirč M, Tomašević Kolarov N, Vukov T, Prokić M. What coloration brings: Implications of background adaptation to oxidative stress in anurans. in Frontiers in Zoology. 2023;20(1):6.
doi:10.1186/s12983-023-00486-z .

Radovanović, Tijana, Petrović, Tamara, Gavrilović, Branka, Despotović, Svetlana, Gavrić, Jelena, Kijanović, Ana, Mirč, Marko, Tomašević Kolarov, Nataša, Vukov, Tanja, Prokić, Marko, "What coloration brings: Implications of background adaptation to oxidative stress in anurans" in Frontiers in Zoology, 20, no. 1 (2023):6,
https://doi.org/10.1186/s12983-023-00486-z . .

TY  - JOUR
AU  - Bani, Luciano
AU  - Orioli, Valerio
AU  - Giacchini, Roberto
AU  - Parenti, Paolo
AU  - Dondina, Olivia
AU  - Prokić, Marko
AU  - Faggio, Caterina
AU  - Campli, Giulia
PY  - 2022
UR  - https://onlinelibrary.wiley.com/doi/10.1111/oik.09292
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5070
AB  - Animal populations are increasingly forced to live in small residual natural or semi-natural areas due to habitat loss and fragmentation. Here, the viability of populations is often compromised by intrinsic threat factors typical of small and isolated populations, such as inbreeding depression, genetic drift and environmental and demographic stochasticity. Under these circumstances, organisms may have low fitness due to inadequate physiological responses needed to face environmental challenges. However, few studies have investigated the relationship between habitat fragmentation and stress defences. In this study, we aimed to test whether an increase in the level of individual inbreeding produced an increase in the antioxidant system response. To this purpose, we genotyped 151 individuals of fire salamander Salamandra salamandra (Amphibia: Urodela) within five sampling populations, located in forest landscapes with different degree of fragmentation in northern Italy. For 113 individuals we also measured the glutathione-S-transferase (GST) and catalase (CAT) enzyme activity. Results showed a significant increase in individual GST activity for increasing levels of inbreeding, whereas no relationship was found for CAT. We also measured acetylcholinesterase to test the possible confounding effects of pesticides that might have occurred in fragmented landscapes with forests interspersed with agricultural areas. However, no difference in this enzyme activity was found among sampling populations. We argue that high levels of GST activity may be symptomatic of oxidative stress derived from inbreeding. An increased frequency of homozygous deleterious alleles due to inbreeding may cause homeostatic alterations and trigger the expression of GST for protection against hydrogen peroxide reactive oxygen species. We suggest using GST as a biomarker for environmental stressors with great caution and not to underestimate that the sources of stress deriving from habitat fragmentation could lead to an unbalance in the oxidative status, possibly increasing population susceptibility to infectious diseases and, potentially, spillover events and zoonoses.
PB  - Hoboken: Wiley
T2  - Oikos
T1  - Can antioxidant responses be induced by habitat fragmentation process?
IS  - 9
VL  - 2022
DO  - 10.1111/oik.09292
SP  - e09292
ER  - 

@article{
author = "Bani, Luciano and Orioli, Valerio and Giacchini, Roberto and Parenti, Paolo and Dondina, Olivia and Prokić, Marko and Faggio, Caterina and Campli, Giulia",
year = "2022",
abstract = "Animal populations are increasingly forced to live in small residual natural or semi-natural areas due to habitat loss and fragmentation. Here, the viability of populations is often compromised by intrinsic threat factors typical of small and isolated populations, such as inbreeding depression, genetic drift and environmental and demographic stochasticity. Under these circumstances, organisms may have low fitness due to inadequate physiological responses needed to face environmental challenges. However, few studies have investigated the relationship between habitat fragmentation and stress defences. In this study, we aimed to test whether an increase in the level of individual inbreeding produced an increase in the antioxidant system response. To this purpose, we genotyped 151 individuals of fire salamander Salamandra salamandra (Amphibia: Urodela) within five sampling populations, located in forest landscapes with different degree of fragmentation in northern Italy. For 113 individuals we also measured the glutathione-S-transferase (GST) and catalase (CAT) enzyme activity. Results showed a significant increase in individual GST activity for increasing levels of inbreeding, whereas no relationship was found for CAT. We also measured acetylcholinesterase to test the possible confounding effects of pesticides that might have occurred in fragmented landscapes with forests interspersed with agricultural areas. However, no difference in this enzyme activity was found among sampling populations. We argue that high levels of GST activity may be symptomatic of oxidative stress derived from inbreeding. An increased frequency of homozygous deleterious alleles due to inbreeding may cause homeostatic alterations and trigger the expression of GST for protection against hydrogen peroxide reactive oxygen species. We suggest using GST as a biomarker for environmental stressors with great caution and not to underestimate that the sources of stress deriving from habitat fragmentation could lead to an unbalance in the oxidative status, possibly increasing population susceptibility to infectious diseases and, potentially, spillover events and zoonoses.",
publisher = "Hoboken: Wiley",
journal = "Oikos",
title = "Can antioxidant responses be induced by habitat fragmentation process?",
number = "9",
volume = "2022",
doi = "10.1111/oik.09292",
pages = "e09292"
}

Bani, L., Orioli, V., Giacchini, R., Parenti, P., Dondina, O., Prokić, M., Faggio, C.,& Campli, G.. (2022). Can antioxidant responses be induced by habitat fragmentation process?. in Oikos
Hoboken: Wiley., 2022(9), e09292.
https://doi.org/10.1111/oik.09292

Bani L, Orioli V, Giacchini R, Parenti P, Dondina O, Prokić M, Faggio C, Campli G. Can antioxidant responses be induced by habitat fragmentation process?. in Oikos. 2022;2022(9):e09292.
doi:10.1111/oik.09292 .

Bani, Luciano, Orioli, Valerio, Giacchini, Roberto, Parenti, Paolo, Dondina, Olivia, Prokić, Marko, Faggio, Caterina, Campli, Giulia, "Can antioxidant responses be induced by habitat fragmentation process?" in Oikos, 2022, no. 9 (2022):e09292,
https://doi.org/10.1111/oik.09292 . .

TY  - JOUR
AU  - Rashidian, Ghasem
AU  - Shahin, Khalid
AU  - Elshopakey, Gehad E.
AU  - Mahboub, Heba H.
AU  - Fahim, Azin
AU  - Elabd, Hiam
AU  - Prokić, Marko
AU  - Faggio, Caterina
PY  - 2022
UR  - https://www.mdpi.com/2077-1312/10/3/325
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4893
AB  - Medicinal plants are increasingly used in aquaculture owing to their beneficial impacts on the health status of farmed fish. The current study was conducted to investigate the effect of nutmeg (Myristica fragrans) extract on growth, immunity, antioxidant parameters, and resistance of common carp (Cyprinus carpio) against Aeromonas hydrophila. In addition, in vitro antibacterial activity of the skin mucus of fish fed on nutmeg extract was evaluated against three major fish pathogenic bacteria through the standard disk diffusion method. Fish (17.27 ± 0.11 g) were divided into four groups and fed on experimental diets containing different levels of nutmeg extract, including zero (control), 0.5% (M1), 1% (M2), and 2% (M3) per kg diet. Results showed that nutmeg significantly enhanced growth parameters after a four-week feeding trial. Feed conversion ratio was remarkably reduced with the lowest value reported for the M3 group, whereas weight gain was notably increased in M2 and M3. No significant effect was found on the hematological profile, including mean corpuscular hemoglobin concentration, mean corpuscular hemoglobin, mean corpuscular volume, and hematocrit, while the highest levels of red blood cells and white blood cells were found in the M3 group. Stress biomarkers, including glucose and cortisol, were the lowest in the M3 group. Serum and skin mucus immunological and antioxidant parameters were significantly higher in M3, followed by M2, where the highest resistance was also observed. In addition, skin mucus samples effectively inhibited Streptococcus iniae, Yersinia ruckeri, and Aeromonas hydrophila. Overall, the present results suggest that dietary nutmeg (20 g/kg diet) could be used as a growth promotor and immunostimulant in common carp.
PB  - Basel: MDPI
T2  - Journal of Marine Science and Engineering
T1  - The Dietary Effects of Nutmeg (Myristica fragrans) Extract on Growth, Hematological Parameters, Immunity, Antioxidant Status, and Disease Resistance of Common Carp (Cyprinus carpio) against Aeromonas hydrophila
IS  - 3
VL  - 10
DO  - 10.3390/jmse10030325
SP  - 325
ER  - 

@article{
author = "Rashidian, Ghasem and Shahin, Khalid and Elshopakey, Gehad E. and Mahboub, Heba H. and Fahim, Azin and Elabd, Hiam and Prokić, Marko and Faggio, Caterina",
year = "2022",
abstract = "Medicinal plants are increasingly used in aquaculture owing to their beneficial impacts on the health status of farmed fish. The current study was conducted to investigate the effect of nutmeg (Myristica fragrans) extract on growth, immunity, antioxidant parameters, and resistance of common carp (Cyprinus carpio) against Aeromonas hydrophila. In addition, in vitro antibacterial activity of the skin mucus of fish fed on nutmeg extract was evaluated against three major fish pathogenic bacteria through the standard disk diffusion method. Fish (17.27 ± 0.11 g) were divided into four groups and fed on experimental diets containing different levels of nutmeg extract, including zero (control), 0.5% (M1), 1% (M2), and 2% (M3) per kg diet. Results showed that nutmeg significantly enhanced growth parameters after a four-week feeding trial. Feed conversion ratio was remarkably reduced with the lowest value reported for the M3 group, whereas weight gain was notably increased in M2 and M3. No significant effect was found on the hematological profile, including mean corpuscular hemoglobin concentration, mean corpuscular hemoglobin, mean corpuscular volume, and hematocrit, while the highest levels of red blood cells and white blood cells were found in the M3 group. Stress biomarkers, including glucose and cortisol, were the lowest in the M3 group. Serum and skin mucus immunological and antioxidant parameters were significantly higher in M3, followed by M2, where the highest resistance was also observed. In addition, skin mucus samples effectively inhibited Streptococcus iniae, Yersinia ruckeri, and Aeromonas hydrophila. Overall, the present results suggest that dietary nutmeg (20 g/kg diet) could be used as a growth promotor and immunostimulant in common carp.",
publisher = "Basel: MDPI",
journal = "Journal of Marine Science and Engineering",
title = "The Dietary Effects of Nutmeg (Myristica fragrans) Extract on Growth, Hematological Parameters, Immunity, Antioxidant Status, and Disease Resistance of Common Carp (Cyprinus carpio) against Aeromonas hydrophila",
number = "3",
volume = "10",
doi = "10.3390/jmse10030325",
pages = "325"
}

Rashidian, G., Shahin, K., Elshopakey, G. E., Mahboub, H. H., Fahim, A., Elabd, H., Prokić, M.,& Faggio, C.. (2022). The Dietary Effects of Nutmeg (Myristica fragrans) Extract on Growth, Hematological Parameters, Immunity, Antioxidant Status, and Disease Resistance of Common Carp (Cyprinus carpio) against Aeromonas hydrophila. in Journal of Marine Science and Engineering
Basel: MDPI., 10(3), 325.
https://doi.org/10.3390/jmse10030325

Rashidian G, Shahin K, Elshopakey GE, Mahboub HH, Fahim A, Elabd H, Prokić M, Faggio C. The Dietary Effects of Nutmeg (Myristica fragrans) Extract on Growth, Hematological Parameters, Immunity, Antioxidant Status, and Disease Resistance of Common Carp (Cyprinus carpio) against Aeromonas hydrophila. in Journal of Marine Science and Engineering. 2022;10(3):325.
doi:10.3390/jmse10030325 .

Rashidian, Ghasem, Shahin, Khalid, Elshopakey, Gehad E., Mahboub, Heba H., Fahim, Azin, Elabd, Hiam, Prokić, Marko, Faggio, Caterina, "The Dietary Effects of Nutmeg (Myristica fragrans) Extract on Growth, Hematological Parameters, Immunity, Antioxidant Status, and Disease Resistance of Common Carp (Cyprinus carpio) against Aeromonas hydrophila" in Journal of Marine Science and Engineering, 10, no. 3 (2022):325,
https://doi.org/10.3390/jmse10030325 . .

TY  - JOUR
AU  - Vali, Sara
AU  - Majidiyan, Nava
AU  - Yalsuyi, Ahmad Mohamadi
AU  - Vajargah, Mohammad Forouhar
AU  - Prokić, Marko
AU  - Faggio, Caterina
PY  - 2022
UR  - https://www.mdpi.com/2073-4441/14/2/144
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4770
AB  - Nanoparticles (NPs) can display toxicological effects on aquatic organisms. This study investigates ecotoxicological effects of Ag-NPs on reproductive and blood parameters of adult common molly (Poecilia sphenops) and their larvae. During the LC50 96 h test, female fish were exposed to concentrations of 0, 5, 15, 25, 35, 45 and 60 mg L−1 of Ag-NPs, while larvae were exposed to 0, 3, 5, 10 and 15 mg L−1. Finally, we aim to evaluate the effects of 0, 5, 10 and 15 mg L−1 of Ag-NPs on parturition time, reproductive success and hematological parameters of the mature fish exposed to sub-lethal concentration during a 62-day period. We also evaluated the survival rate of larvae. The results show a positive correlation between mortality rate and Ag-NP concentration. Values for LC50 96 h in adult fish and larvae were 26.85 mg L−1 and 6.22 mg L−1, respectively. A lack of parturition and reproductive success were seen in fish that underwent chronic exposure to Ag-NPs (15 mg L−1). The results show that RBC, WBC and hematocrit were significantly decreased in fish exposed to Ag-NPs. In addition, the serum concentrations of total protein, albumin, cholesterol and triglycerides were significantly increased in fish submitted to Ag-NPs (concentrations of 5–15). In conclusion, submitting a fish to higher concentration than 10 mg L−1 has adverse effects on reproductive system and blood parameters.
PB  - Basel: MDPI
T2  - Water
T1  - Ecotoxicological Effects of Silver Nanoparticles (Ag-NPs) on Parturition Time, Survival Rate, Reproductive Success and Blood Parameters of Adult Common Molly (Poecilia sphenops) and Their Larvae
IS  - 2
VL  - 14
DO  - 10.3390/w14020144
SP  - 144
ER  - 

@article{
author = "Vali, Sara and Majidiyan, Nava and Yalsuyi, Ahmad Mohamadi and Vajargah, Mohammad Forouhar and Prokić, Marko and Faggio, Caterina",
year = "2022",
abstract = "Nanoparticles (NPs) can display toxicological effects on aquatic organisms. This study investigates ecotoxicological effects of Ag-NPs on reproductive and blood parameters of adult common molly (Poecilia sphenops) and their larvae. During the LC50 96 h test, female fish were exposed to concentrations of 0, 5, 15, 25, 35, 45 and 60 mg L−1 of Ag-NPs, while larvae were exposed to 0, 3, 5, 10 and 15 mg L−1. Finally, we aim to evaluate the effects of 0, 5, 10 and 15 mg L−1 of Ag-NPs on parturition time, reproductive success and hematological parameters of the mature fish exposed to sub-lethal concentration during a 62-day period. We also evaluated the survival rate of larvae. The results show a positive correlation between mortality rate and Ag-NP concentration. Values for LC50 96 h in adult fish and larvae were 26.85 mg L−1 and 6.22 mg L−1, respectively. A lack of parturition and reproductive success were seen in fish that underwent chronic exposure to Ag-NPs (15 mg L−1). The results show that RBC, WBC and hematocrit were significantly decreased in fish exposed to Ag-NPs. In addition, the serum concentrations of total protein, albumin, cholesterol and triglycerides were significantly increased in fish submitted to Ag-NPs (concentrations of 5–15). In conclusion, submitting a fish to higher concentration than 10 mg L−1 has adverse effects on reproductive system and blood parameters.",
publisher = "Basel: MDPI",
journal = "Water",
title = "Ecotoxicological Effects of Silver Nanoparticles (Ag-NPs) on Parturition Time, Survival Rate, Reproductive Success and Blood Parameters of Adult Common Molly (Poecilia sphenops) and Their Larvae",
number = "2",
volume = "14",
doi = "10.3390/w14020144",
pages = "144"
}

Vali, S., Majidiyan, N., Yalsuyi, A. M., Vajargah, M. F., Prokić, M.,& Faggio, C.. (2022). Ecotoxicological Effects of Silver Nanoparticles (Ag-NPs) on Parturition Time, Survival Rate, Reproductive Success and Blood Parameters of Adult Common Molly (Poecilia sphenops) and Their Larvae. in Water
Basel: MDPI., 14(2), 144.
https://doi.org/10.3390/w14020144

Vali S, Majidiyan N, Yalsuyi AM, Vajargah MF, Prokić M, Faggio C. Ecotoxicological Effects of Silver Nanoparticles (Ag-NPs) on Parturition Time, Survival Rate, Reproductive Success and Blood Parameters of Adult Common Molly (Poecilia sphenops) and Their Larvae. in Water. 2022;14(2):144.
doi:10.3390/w14020144 .

Vali, Sara, Majidiyan, Nava, Yalsuyi, Ahmad Mohamadi, Vajargah, Mohammad Forouhar, Prokić, Marko, Faggio, Caterina, "Ecotoxicological Effects of Silver Nanoparticles (Ag-NPs) on Parturition Time, Survival Rate, Reproductive Success and Blood Parameters of Adult Common Molly (Poecilia sphenops) and Their Larvae" in Water, 14, no. 2 (2022):144,
https://doi.org/10.3390/w14020144 . .

TY  - JOUR
AU  - Rashidian, Ghasem
AU  - Mahboub, Heba H.
AU  - Fahim, Azin
AU  - Hefny, Ahmed A.
AU  - Prokić, Marko
AU  - Rainis, Simona
AU  - Boldaji, Javad Tahmasebi
AU  - Faggio, Caterina
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S1050464821004289
UR  - http://www.ncbi.nlm.nih.gov/pubmed/34910977
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4747
AB  - In large-scale aquaculture, the fast growth rate of fish is positively influenced by feed additives such as medicinal plants. This is however; infectious disease may reduce fish growth and cause devastating economic loss. The present study investigated in vitro antibacterial efficacy of Mooseer (Allium hirtifolium) extract against Streptococcus iniae and its in vivo effects on growth, biochemical parameters, innate immunity of rainbow trout (Oncorhynchus mykiss). Therefore, six experimental diets were designed to include different levels of Mooseer from zero (as control), 5, 10, 15, 20, and 25 g per kg diet respectively referred to as M1 to M5. Results from the antibacterial evaluation showed that Mooseer extract inhibits S. iniae growth with MIC and MBC values of 128 and 256 μg ml-1. Appreciable results were obtained in the groups supplemented with Mooseer. Mooseer enhanced growth performance, and modulated serum biochemical and immunological parameters (total protein, albumin, triglyceride, glucose, cortisol, cholesterol, lysozyme, Ig, ACH50, ALP, and protease activity), and liver enzymes (ALT, AST and ALP). The greatest effects were found for higher doses of Mooseer supplementation (M4 and M5). Meanwhile, results from the survival rate of fish challenged with S. iniae showed higher survival in M2 and M4 treatments. The present findings suggest the beneficial use of Mooseer in rainbow trout diet, with 20 g kg-1 inclusion as the recommended dose.
PB  - London: Academic Press
T2  - Fish & Shellfish Immunology
T1  - Mooseer (Allium hirtifolium) boosts growth, general health status, and resistance of rainbow trout (Oncorhynchus mykiss) against Streptococcus iniae infection.
VL  - 120
DO  - 10.1016/j.fsi.2021.12.012
SP  - 360
EP  - 368
ER  - 

@article{
author = "Rashidian, Ghasem and Mahboub, Heba H. and Fahim, Azin and Hefny, Ahmed A. and Prokić, Marko and Rainis, Simona and Boldaji, Javad Tahmasebi and Faggio, Caterina",
year = "2022",
abstract = "In large-scale aquaculture, the fast growth rate of fish is positively influenced by feed additives such as medicinal plants. This is however; infectious disease may reduce fish growth and cause devastating economic loss. The present study investigated in vitro antibacterial efficacy of Mooseer (Allium hirtifolium) extract against Streptococcus iniae and its in vivo effects on growth, biochemical parameters, innate immunity of rainbow trout (Oncorhynchus mykiss). Therefore, six experimental diets were designed to include different levels of Mooseer from zero (as control), 5, 10, 15, 20, and 25 g per kg diet respectively referred to as M1 to M5. Results from the antibacterial evaluation showed that Mooseer extract inhibits S. iniae growth with MIC and MBC values of 128 and 256 μg ml-1. Appreciable results were obtained in the groups supplemented with Mooseer. Mooseer enhanced growth performance, and modulated serum biochemical and immunological parameters (total protein, albumin, triglyceride, glucose, cortisol, cholesterol, lysozyme, Ig, ACH50, ALP, and protease activity), and liver enzymes (ALT, AST and ALP). The greatest effects were found for higher doses of Mooseer supplementation (M4 and M5). Meanwhile, results from the survival rate of fish challenged with S. iniae showed higher survival in M2 and M4 treatments. The present findings suggest the beneficial use of Mooseer in rainbow trout diet, with 20 g kg-1 inclusion as the recommended dose.",
publisher = "London: Academic Press",
journal = "Fish & Shellfish Immunology",
title = "Mooseer (Allium hirtifolium) boosts growth, general health status, and resistance of rainbow trout (Oncorhynchus mykiss) against Streptococcus iniae infection.",
volume = "120",
doi = "10.1016/j.fsi.2021.12.012",
pages = "360-368"
}

Rashidian, G., Mahboub, H. H., Fahim, A., Hefny, A. A., Prokić, M., Rainis, S., Boldaji, J. T.,& Faggio, C.. (2022). Mooseer (Allium hirtifolium) boosts growth, general health status, and resistance of rainbow trout (Oncorhynchus mykiss) against Streptococcus iniae infection.. in Fish & Shellfish Immunology
London: Academic Press., 120, 360-368.
https://doi.org/10.1016/j.fsi.2021.12.012

Rashidian G, Mahboub HH, Fahim A, Hefny AA, Prokić M, Rainis S, Boldaji JT, Faggio C. Mooseer (Allium hirtifolium) boosts growth, general health status, and resistance of rainbow trout (Oncorhynchus mykiss) against Streptococcus iniae infection.. in Fish & Shellfish Immunology. 2022;120:360-368.
doi:10.1016/j.fsi.2021.12.012 .

Rashidian, Ghasem, Mahboub, Heba H., Fahim, Azin, Hefny, Ahmed A., Prokić, Marko, Rainis, Simona, Boldaji, Javad Tahmasebi, Faggio, Caterina, "Mooseer (Allium hirtifolium) boosts growth, general health status, and resistance of rainbow trout (Oncorhynchus mykiss) against Streptococcus iniae infection." in Fish & Shellfish Immunology, 120 (2022):360-368,
https://doi.org/10.1016/j.fsi.2021.12.012 . .

TY  - CONF
AU  - Kijanović, Ana
AU  - Vukov, Tanja
AU  - Mirč, Marko
AU  - Mitrović, Aleksandar
AU  - Prokić, Marko
AU  - Radovanović, Tijana
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Petrović, Tamara
AU  - Tomašević Kolarov, Nataša
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5250
AB  - Many amphibian species inhabit temporary aquatic habitats for reproduction to
maximize larval growth under favorable conditions but accelerate developmental rate
to rapidly undergo metamorphosis under stress conditions when high desiccation risk
exists. Corticosterone, one of the most important vertebrate stress hormone controls
development, metabolism, and skeletal growth and together with thyroid hormone
have a critical role in anuran metamorphosis under stress conditions. Here we
compare the hormonal variation of corticosterone (CORT) in the yellow-bellied toad
Bombina variegata tadpoles in response to different water levels (constant highcontrol, constant low, constant high+exogenous CORT) and the corticosterone
inhibitor- metyrapone (MET) (treatment constant low water level+MET). Also, we
investigated the effects of CORT and MET on life-history and morphological traits at
metamorphosis. We found that B. variegata tadpoles cannot accelerate the
developmental rate in response to pond drying. The constant low water level did not
alter whole-body content of CORT and it did not significantly differ from high water
level treatment with exogenous CORT. However, individuals from high water level with
exogenous CORT have prolonged development, the highest mortality rate, the smallest
body size and mass, and the widest but the shortest tails at metamorphosis. Constant
low water level with MET decreased the whole-body content of CORT and it was
significantly different between all groups, but it did not affect the developmental rate
and tail shape although these tadpoles have intermediate size and mass between high
water levels with exogenous CORT and control. We show that responsiveness to pond
drying of Bombina variegata and its short larval period is probably associated with
modified endocrine signaling pathway that control tadpole metamorphosis.
Constitutively high whole-body content of CORT which was unaltered by pond drying
leads us to conclusion that Bombina variegata development may be canalized but more
research is needed.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
T1  - Desiccation stress response of Bombina variegata tadpoles
SP  - 158
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5250
ER  - 

@conference{
author = "Kijanović, Ana and Vukov, Tanja and Mirč, Marko and Mitrović, Aleksandar and Prokić, Marko and Radovanović, Tijana and Gavrilović, Branka and Despotović, Svetlana and Gavrić, Jelena and Petrović, Tamara and Tomašević Kolarov, Nataša",
year = "2022",
abstract = "Many amphibian species inhabit temporary aquatic habitats for reproduction to
maximize larval growth under favorable conditions but accelerate developmental rate
to rapidly undergo metamorphosis under stress conditions when high desiccation risk
exists. Corticosterone, one of the most important vertebrate stress hormone controls
development, metabolism, and skeletal growth and together with thyroid hormone
have a critical role in anuran metamorphosis under stress conditions. Here we
compare the hormonal variation of corticosterone (CORT) in the yellow-bellied toad
Bombina variegata tadpoles in response to different water levels (constant highcontrol, constant low, constant high+exogenous CORT) and the corticosterone
inhibitor- metyrapone (MET) (treatment constant low water level+MET). Also, we
investigated the effects of CORT and MET on life-history and morphological traits at
metamorphosis. We found that B. variegata tadpoles cannot accelerate the
developmental rate in response to pond drying. The constant low water level did not
alter whole-body content of CORT and it did not significantly differ from high water
level treatment with exogenous CORT. However, individuals from high water level with
exogenous CORT have prolonged development, the highest mortality rate, the smallest
body size and mass, and the widest but the shortest tails at metamorphosis. Constant
low water level with MET decreased the whole-body content of CORT and it was
significantly different between all groups, but it did not affect the developmental rate
and tail shape although these tadpoles have intermediate size and mass between high
water levels with exogenous CORT and control. We show that responsiveness to pond
drying of Bombina variegata and its short larval period is probably associated with
modified endocrine signaling pathway that control tadpole metamorphosis.
Constitutively high whole-body content of CORT which was unaltered by pond drying
leads us to conclusion that Bombina variegata development may be canalized but more
research is needed.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia",
title = "Desiccation stress response of Bombina variegata tadpoles",
pages = "158",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5250"
}

Kijanović, A., Vukov, T., Mirč, M., Mitrović, A., Prokić, M., Radovanović, T., Gavrilović, B., Despotović, S., Gavrić, J., Petrović, T.,& Tomašević Kolarov, N.. (2022). Desiccation stress response of Bombina variegata tadpoles. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 158.
https://hdl.handle.net/21.15107/rcub_ibiss_5250

Kijanović A, Vukov T, Mirč M, Mitrović A, Prokić M, Radovanović T, Gavrilović B, Despotović S, Gavrić J, Petrović T, Tomašević Kolarov N. Desiccation stress response of Bombina variegata tadpoles. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. 2022;:158.
https://hdl.handle.net/21.15107/rcub_ibiss_5250 .

Kijanović, Ana, Vukov, Tanja, Mirč, Marko, Mitrović, Aleksandar, Prokić, Marko, Radovanović, Tijana, Gavrilović, Branka, Despotović, Svetlana, Gavrić, Jelena, Petrović, Tamara, Tomašević Kolarov, Nataša, "Desiccation stress response of Bombina variegata tadpoles" in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia (2022):158,
https://hdl.handle.net/21.15107/rcub_ibiss_5250 .

TY  - JOUR
AU  - Rashidian, Ghasem
AU  - Tahmasebi Boldaji, Javad
AU  - Rainis, Simona
AU  - Prokić, Marko
AU  - Faggio, Caterina
PY  - 2021
UR  - internal-pdf://Rashidian et al. - 2021 - Oregano (Origanum vulgare) Extract Enhances Zebrafish (Danio rerio) Growth Performance, Serum and Mucus Innate.pdf
UR  - https://www.mdpi.com/2076-2615/11/2/299
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4139
AB  - This study evaluated the dietary effects of an ultrasound-assisted extract of Origanum vulgare on the growth, antioxidant and immune responses (serum and mucosal) and resistance of zebrafish (Danio rerio) against Aeromonas hydrophila. Four hundred and forty adult zebrafish were distributed into 12 tanks and fed 4 experimental diets including 0% (control), 0.5% (M1), 1% (M2) and 2% (M3) of the extract per kg−1 diet for eight weeks. Fish were then challenged with A. hydrophila and mortality was recorded for 10 days. Results revealed that the extract exerted potent effects on growth parameters of weight gain and specific growth rate. The feed conversion ratio was significantly lower in fish fed extract-incorporated diets. O. vulgare extract improved antioxidant and immune responses, resulting in less sensitivity to oxidative stress and a higher survival rate when challenged with A. hydrophila. Overall, the greatest effects were observed in individuals with 1% dietary inclusion of the extract. These results suggest that the extract from the plant Origanum vulgare possesses a great potential to be used in the aquaculture industry and that zebrafish is an appropriate model for nutrition studies.
PB  - MDPI AG
T2  - Animals
T1  - Oregano (Origanum vulgare) Extract Enhances Zebrafish (Danio rerio) Growth Performance, Serum and Mucus Innate Immune Responses and Resistance against Aeromonas hydrophila Challenge
IS  - 2
VL  - 11
DO  - 10.3390/ani11020299
SP  - 299
ER  - 

@article{
author = "Rashidian, Ghasem and Tahmasebi Boldaji, Javad and Rainis, Simona and Prokić, Marko and Faggio, Caterina",
year = "2021",
abstract = "This study evaluated the dietary effects of an ultrasound-assisted extract of Origanum vulgare on the growth, antioxidant and immune responses (serum and mucosal) and resistance of zebrafish (Danio rerio) against Aeromonas hydrophila. Four hundred and forty adult zebrafish were distributed into 12 tanks and fed 4 experimental diets including 0% (control), 0.5% (M1), 1% (M2) and 2% (M3) of the extract per kg−1 diet for eight weeks. Fish were then challenged with A. hydrophila and mortality was recorded for 10 days. Results revealed that the extract exerted potent effects on growth parameters of weight gain and specific growth rate. The feed conversion ratio was significantly lower in fish fed extract-incorporated diets. O. vulgare extract improved antioxidant and immune responses, resulting in less sensitivity to oxidative stress and a higher survival rate when challenged with A. hydrophila. Overall, the greatest effects were observed in individuals with 1% dietary inclusion of the extract. These results suggest that the extract from the plant Origanum vulgare possesses a great potential to be used in the aquaculture industry and that zebrafish is an appropriate model for nutrition studies.",
publisher = "MDPI AG",
journal = "Animals",
title = "Oregano (Origanum vulgare) Extract Enhances Zebrafish (Danio rerio) Growth Performance, Serum and Mucus Innate Immune Responses and Resistance against Aeromonas hydrophila Challenge",
number = "2",
volume = "11",
doi = "10.3390/ani11020299",
pages = "299"
}

Rashidian, G., Tahmasebi Boldaji, J., Rainis, S., Prokić, M.,& Faggio, C.. (2021). Oregano (Origanum vulgare) Extract Enhances Zebrafish (Danio rerio) Growth Performance, Serum and Mucus Innate Immune Responses and Resistance against Aeromonas hydrophila Challenge. in Animals
MDPI AG., 11(2), 299.
https://doi.org/10.3390/ani11020299

Rashidian G, Tahmasebi Boldaji J, Rainis S, Prokić M, Faggio C. Oregano (Origanum vulgare) Extract Enhances Zebrafish (Danio rerio) Growth Performance, Serum and Mucus Innate Immune Responses and Resistance against Aeromonas hydrophila Challenge. in Animals. 2021;11(2):299.
doi:10.3390/ani11020299 .

Rashidian, Ghasem, Tahmasebi Boldaji, Javad, Rainis, Simona, Prokić, Marko, Faggio, Caterina, "Oregano (Origanum vulgare) Extract Enhances Zebrafish (Danio rerio) Growth Performance, Serum and Mucus Innate Immune Responses and Resistance against Aeromonas hydrophila Challenge" in Animals, 11, no. 2 (2021):299,
https://doi.org/10.3390/ani11020299 . .

TY  - JOUR
AU  - Prokić, Marko
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Gavrić, Jelena
AU  - Petrović, Tamara
AU  - Despotović, Svetlana
AU  - Faggio, Caterina
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4164
AB  - Although we are witnesses of an increase in the number of studies examining the exposure/effects of microplastics (MPs) on different organisms, there are many unknowns. This review aims to: (i) analyze current studies devoted to investigating the exposure/effects of MPs on animals; (ii) provide some basic knowledge about different model organisms and experimental approaches used in studying MPs; and to (iii) convey directions for future studies. We have summarized data from 500 studies published from January 2011 to May 2020, about different aspects of model organisms (taxonomic group of organisms, type of ecosystem they inhabit, life-stage, sex, tissue and/or organ) and experimental design (laboratory/field, ingestion/bioaccumulation/effect). We also discuss and try to encourage investigation of some less studied organisms (terrestrial and freshwater species, among groups including Annelida, Nematoda, Echinodermata, Cnidaria, Rotifera, birds, amphibians, reptiles), and aspects of MP pollution (long-term field studies, comparative studies examining life stages, sexes, laboratory and field work). We hope that the information presented in this review will serve as a good starting point and will provide useful guidelines for researchers during the process of deciding on the model organism and study designs for investigating MPs.
PB  - Elsevier B.V.
T2  - Journal of Hazardous Materials
T1  - Studying microplastics: Lessons from evaluated literature on animal model organisms and experimental approaches
VL  - 414
DO  - 10.1016/j.jhazmat.2021.125476
SP  - 125476
ER  - 

@article{
author = "Prokić, Marko and Gavrilović, Branka and Radovanović, Tijana and Gavrić, Jelena and Petrović, Tamara and Despotović, Svetlana and Faggio, Caterina",
year = "2021",
abstract = "Although we are witnesses of an increase in the number of studies examining the exposure/effects of microplastics (MPs) on different organisms, there are many unknowns. This review aims to: (i) analyze current studies devoted to investigating the exposure/effects of MPs on animals; (ii) provide some basic knowledge about different model organisms and experimental approaches used in studying MPs; and to (iii) convey directions for future studies. We have summarized data from 500 studies published from January 2011 to May 2020, about different aspects of model organisms (taxonomic group of organisms, type of ecosystem they inhabit, life-stage, sex, tissue and/or organ) and experimental design (laboratory/field, ingestion/bioaccumulation/effect). We also discuss and try to encourage investigation of some less studied organisms (terrestrial and freshwater species, among groups including Annelida, Nematoda, Echinodermata, Cnidaria, Rotifera, birds, amphibians, reptiles), and aspects of MP pollution (long-term field studies, comparative studies examining life stages, sexes, laboratory and field work). We hope that the information presented in this review will serve as a good starting point and will provide useful guidelines for researchers during the process of deciding on the model organism and study designs for investigating MPs.",
publisher = "Elsevier B.V.",
journal = "Journal of Hazardous Materials",
title = "Studying microplastics: Lessons from evaluated literature on animal model organisms and experimental approaches",
volume = "414",
doi = "10.1016/j.jhazmat.2021.125476",
pages = "125476"
}

Prokić, M., Gavrilović, B., Radovanović, T., Gavrić, J., Petrović, T., Despotović, S.,& Faggio, C.. (2021). Studying microplastics: Lessons from evaluated literature on animal model organisms and experimental approaches. in Journal of Hazardous Materials
Elsevier B.V.., 414, 125476.
https://doi.org/10.1016/j.jhazmat.2021.125476

Prokić M, Gavrilović B, Radovanović T, Gavrić J, Petrović T, Despotović S, Faggio C. Studying microplastics: Lessons from evaluated literature on animal model organisms and experimental approaches. in Journal of Hazardous Materials. 2021;414:125476.
doi:10.1016/j.jhazmat.2021.125476 .

Prokić, Marko, Gavrilović, Branka, Radovanović, Tijana, Gavrić, Jelena, Petrović, Tamara, Despotović, Svetlana, Faggio, Caterina, "Studying microplastics: Lessons from evaluated literature on animal model organisms and experimental approaches" in Journal of Hazardous Materials, 414 (2021):125476,
https://doi.org/10.1016/j.jhazmat.2021.125476 . .

TY  - JOUR
AU  - Prokić, Marko
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Gavrić, Jelena
AU  - Petrović, Tamara
AU  - Despotović, Svetlana
AU  - Faggio, Caterina
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4164
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4165
AB  - Although we are witnesses of an increase in the number of studies examining the exposure/effects of microplastics (MPs) on different organisms, there are many unknowns. This review aims to: (i) analyze current studies devoted to investigating the exposure/effects of MPs on animals; (ii) provide some basic knowledge about different model organisms and experimental approaches used in studying MPs; and to (iii) convey directions for future studies. We have summarized data from 500 studies published from January 2011 to May 2020, about different aspects of model organisms (taxonomic group of organisms, type of ecosystem they inhabit, life-stage, sex, tissue and/or organ) and experimental design (laboratory/field, ingestion/bioaccumulation/effect). We also discuss and try to encourage investigation of some less studied organisms (terrestrial and freshwater species, among groups including Annelida, Nematoda, Echinodermata, Cnidaria, Rotifera, birds, amphibians, reptiles), and aspects of MP pollution (long-term field studies, comparative studies examining life stages, sexes, laboratory and field work). We hope that the information presented in this review will serve as a good starting point and will provide useful guidelines for researchers during the process of deciding on the model organism and study designs for investigating MPs.
PB  - Elsevier B.V.
T2  - Journal of Hazardous Materials
T1  - Studying microplastics: Lessons from evaluated literature on animal model organisms and experimental approaches
VL  - 414
DO  - 10.1016/j.jhazmat.2021.125476
SP  - 125476
ER  - 

@article{
author = "Prokić, Marko and Gavrilović, Branka and Radovanović, Tijana and Gavrić, Jelena and Petrović, Tamara and Despotović, Svetlana and Faggio, Caterina",
year = "2021",
abstract = "Although we are witnesses of an increase in the number of studies examining the exposure/effects of microplastics (MPs) on different organisms, there are many unknowns. This review aims to: (i) analyze current studies devoted to investigating the exposure/effects of MPs on animals; (ii) provide some basic knowledge about different model organisms and experimental approaches used in studying MPs; and to (iii) convey directions for future studies. We have summarized data from 500 studies published from January 2011 to May 2020, about different aspects of model organisms (taxonomic group of organisms, type of ecosystem they inhabit, life-stage, sex, tissue and/or organ) and experimental design (laboratory/field, ingestion/bioaccumulation/effect). We also discuss and try to encourage investigation of some less studied organisms (terrestrial and freshwater species, among groups including Annelida, Nematoda, Echinodermata, Cnidaria, Rotifera, birds, amphibians, reptiles), and aspects of MP pollution (long-term field studies, comparative studies examining life stages, sexes, laboratory and field work). We hope that the information presented in this review will serve as a good starting point and will provide useful guidelines for researchers during the process of deciding on the model organism and study designs for investigating MPs.",
publisher = "Elsevier B.V.",
journal = "Journal of Hazardous Materials",
title = "Studying microplastics: Lessons from evaluated literature on animal model organisms and experimental approaches",
volume = "414",
doi = "10.1016/j.jhazmat.2021.125476",
pages = "125476"
}

Prokić, M., Gavrilović, B., Radovanović, T., Gavrić, J., Petrović, T., Despotović, S.,& Faggio, C.. (2021). Studying microplastics: Lessons from evaluated literature on animal model organisms and experimental approaches. in Journal of Hazardous Materials
Elsevier B.V.., 414, 125476.
https://doi.org/10.1016/j.jhazmat.2021.125476

Prokić M, Gavrilović B, Radovanović T, Gavrić J, Petrović T, Despotović S, Faggio C. Studying microplastics: Lessons from evaluated literature on animal model organisms and experimental approaches. in Journal of Hazardous Materials. 2021;414:125476.
doi:10.1016/j.jhazmat.2021.125476 .

Prokić, Marko, Gavrilović, Branka, Radovanović, Tijana, Gavrić, Jelena, Petrović, Tamara, Despotović, Svetlana, Faggio, Caterina, "Studying microplastics: Lessons from evaluated literature on animal model organisms and experimental approaches" in Journal of Hazardous Materials, 414 (2021):125476,
https://doi.org/10.1016/j.jhazmat.2021.125476 . .

TY  - JOUR
AU  - Petrović, Tamara
AU  - Kijanović, Ana
AU  - Tomašević Kolarov, Nataša
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Vukov, Tanja
AU  - Faggio, Caterina
AU  - Prokić, Marko
PY  - 2021
UR  - https://www.mdpi.com/2076-2615/11/4/953
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4192
AB  - In this paper, we examined how the oxidative status (antioxidant system and oxidative damage) of Bombina variegata larvae changed during the metamorphic climax (Gosner stages: 42—beginning, 44—middle and 46—end) and compared the patterns and levels of oxidative stress parameters between individuals developing under constant water availability (control) and those developing under decreasing water availability (desiccation group). Our results revealed that larvae developing under decreasing water availability exhibited increased oxidative damage in the middle and end stages. This was followed by lower levels of glutathione in stages 44 and 46, as well as lower values of catalase, glutathione peroxidase, glutathione S-transferase and sulfhydryl groups in stage 46 (all in relation to control animals). Comparison between stages 42, 44 and 46 within treatments showed that individuals in the last stage demonstrated the highest intensities of lipid oxidative damage in both the control and desiccation groups. As for the parameters of the antioxidant system, control individuals displayed greater variety in response to changes induced by metamorphic climax than individuals exposed to desiccation treatment. The overall decrease in water availability during development led to increased oxidative stress and modifications in the pattern of AOS response to changes induced by metamorphic climax in larvae of B. variegata.
PB  - MDPI AG
T2  - Animals
T1  - Effects of Desiccation on Metamorphic Climax in Bombina variegata: Changes in Levels and Patterns of Oxidative Stress Parameters
IS  - 4
VL  - 11
DO  - 10.3390/ani11040953
SP  - 953
ER  - 

@article{
author = "Petrović, Tamara and Kijanović, Ana and Tomašević Kolarov, Nataša and Gavrić, Jelena and Despotović, Svetlana and Gavrilović, Branka and Radovanović, Tijana and Vukov, Tanja and Faggio, Caterina and Prokić, Marko",
year = "2021",
abstract = "In this paper, we examined how the oxidative status (antioxidant system and oxidative damage) of Bombina variegata larvae changed during the metamorphic climax (Gosner stages: 42—beginning, 44—middle and 46—end) and compared the patterns and levels of oxidative stress parameters between individuals developing under constant water availability (control) and those developing under decreasing water availability (desiccation group). Our results revealed that larvae developing under decreasing water availability exhibited increased oxidative damage in the middle and end stages. This was followed by lower levels of glutathione in stages 44 and 46, as well as lower values of catalase, glutathione peroxidase, glutathione S-transferase and sulfhydryl groups in stage 46 (all in relation to control animals). Comparison between stages 42, 44 and 46 within treatments showed that individuals in the last stage demonstrated the highest intensities of lipid oxidative damage in both the control and desiccation groups. As for the parameters of the antioxidant system, control individuals displayed greater variety in response to changes induced by metamorphic climax than individuals exposed to desiccation treatment. The overall decrease in water availability during development led to increased oxidative stress and modifications in the pattern of AOS response to changes induced by metamorphic climax in larvae of B. variegata.",
publisher = "MDPI AG",
journal = "Animals",
title = "Effects of Desiccation on Metamorphic Climax in Bombina variegata: Changes in Levels and Patterns of Oxidative Stress Parameters",
number = "4",
volume = "11",
doi = "10.3390/ani11040953",
pages = "953"
}

Petrović, T., Kijanović, A., Tomašević Kolarov, N., Gavrić, J., Despotović, S., Gavrilović, B., Radovanović, T., Vukov, T., Faggio, C.,& Prokić, M.. (2021). Effects of Desiccation on Metamorphic Climax in Bombina variegata: Changes in Levels and Patterns of Oxidative Stress Parameters. in Animals
MDPI AG., 11(4), 953.
https://doi.org/10.3390/ani11040953

Petrović T, Kijanović A, Tomašević Kolarov N, Gavrić J, Despotović S, Gavrilović B, Radovanović T, Vukov T, Faggio C, Prokić M. Effects of Desiccation on Metamorphic Climax in Bombina variegata: Changes in Levels and Patterns of Oxidative Stress Parameters. in Animals. 2021;11(4):953.
doi:10.3390/ani11040953 .

Petrović, Tamara, Kijanović, Ana, Tomašević Kolarov, Nataša, Gavrić, Jelena, Despotović, Svetlana, Gavrilović, Branka, Radovanović, Tijana, Vukov, Tanja, Faggio, Caterina, Prokić, Marko, "Effects of Desiccation on Metamorphic Climax in Bombina variegata: Changes in Levels and Patterns of Oxidative Stress Parameters" in Animals, 11, no. 4 (2021):953,
https://doi.org/10.3390/ani11040953 . .

TY  - JOUR
AU  - Yalsuyi, Ahmad Mohamadi
AU  - Hajimoradloo, Abdolmajid
AU  - Ghorbani, Rasul
AU  - Jafari, Vally-allah
AU  - Prokić, Marko
AU  - Faggio, Caterina
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4215
AB  - In the study, we assessed how acute changes in water temperature and dissolved ammonia concentration can affect the swimming behavior pattern of rainbow trout (Oncorhynchus mykiss). The behavior was analyzed in three different stages: 1) increase stage, (where temperature and ammonia concentration increase during this step, respectively); 2) unchanged stage, (where levels of both factors do not change during this stage); 3) reduction stage, (where ammonia concentration and temperature during this stage are reduced), respectively. The results showed that both factors significantly changed the swimming pattern of the rainbow trout. There were significant differences in swimming parameters (distance from the center, swimming speed, total movement and the average of angular changes of movement) of treated fish in the comparison between treatments, and with the control group. The changes in the swimming pattern of fish in response to physicochemical parameters of water were confirmed to be a good tool in ecotoxicological studies.
PB  - Elsevier BV
T2  - Environmental Toxicology and Pharmacology
T1  - Behavior evaluation of rainbow trout (Oncorhynchus mykiss) following temperature and ammonia alterations.
VL  - 86
DO  - 10.1016/j.etap.2021.103648
SP  - 103648
ER  - 

@article{
author = "Yalsuyi, Ahmad Mohamadi and Hajimoradloo, Abdolmajid and Ghorbani, Rasul and Jafari, Vally-allah and Prokić, Marko and Faggio, Caterina",
year = "2021",
abstract = "In the study, we assessed how acute changes in water temperature and dissolved ammonia concentration can affect the swimming behavior pattern of rainbow trout (Oncorhynchus mykiss). The behavior was analyzed in three different stages: 1) increase stage, (where temperature and ammonia concentration increase during this step, respectively); 2) unchanged stage, (where levels of both factors do not change during this stage); 3) reduction stage, (where ammonia concentration and temperature during this stage are reduced), respectively. The results showed that both factors significantly changed the swimming pattern of the rainbow trout. There were significant differences in swimming parameters (distance from the center, swimming speed, total movement and the average of angular changes of movement) of treated fish in the comparison between treatments, and with the control group. The changes in the swimming pattern of fish in response to physicochemical parameters of water were confirmed to be a good tool in ecotoxicological studies.",
publisher = "Elsevier BV",
journal = "Environmental Toxicology and Pharmacology",
title = "Behavior evaluation of rainbow trout (Oncorhynchus mykiss) following temperature and ammonia alterations.",
volume = "86",
doi = "10.1016/j.etap.2021.103648",
pages = "103648"
}

Yalsuyi, A. M., Hajimoradloo, A., Ghorbani, R., Jafari, V., Prokić, M.,& Faggio, C.. (2021). Behavior evaluation of rainbow trout (Oncorhynchus mykiss) following temperature and ammonia alterations.. in Environmental Toxicology and Pharmacology
Elsevier BV., 86, 103648.
https://doi.org/10.1016/j.etap.2021.103648

Yalsuyi AM, Hajimoradloo A, Ghorbani R, Jafari V, Prokić M, Faggio C. Behavior evaluation of rainbow trout (Oncorhynchus mykiss) following temperature and ammonia alterations.. in Environmental Toxicology and Pharmacology. 2021;86:103648.
doi:10.1016/j.etap.2021.103648 .

Yalsuyi, Ahmad Mohamadi, Hajimoradloo, Abdolmajid, Ghorbani, Rasul, Jafari, Vally-allah, Prokić, Marko, Faggio, Caterina, "Behavior evaluation of rainbow trout (Oncorhynchus mykiss) following temperature and ammonia alterations." in Environmental Toxicology and Pharmacology, 86 (2021):103648,
https://doi.org/10.1016/j.etap.2021.103648 . .

TY  - JOUR
AU  - Vajargah, Mohammad Forouhar
AU  - Namin, Javid Imanpour
AU  - Mohsenpour, Reza
AU  - Yalsuyi, Ahmad Mohammadi
AU  - Prokić, Marko
AU  - Faggio, Caterina
PY  - 2021
UR  - https://link.springer.com/10.1007/s11259-021-09818-y
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4458
AB  - This study investigates acute toxicity and histological effects of Lindane insecticide on intestinal tissues of immature grass carp (Ctenopharyngodon idella). For these purposes, 105 fish were exposed to different concentrations of Lindane for 96 h in 5 groups with 3 replications. Samples of intestinal tissues were prepared in both control and exposure groups during and after the toxicity test. Vaculation, necrosis, bleeding and epithelial degeneration were apparent in the intestine tissue of treated individuals. The LC50 96 h of Lindane was 0.788 ppm in the present study, also the results showed besides pesticide-induced lesions, symptoms of abnormal swimming, anxiety, tendency to swim near the surface, and death due to terminal toxicity with mouth widely open. The exposed fish were pale in color, showed oxygen deficit and severe respiratory problems. Overall, our results confirm the toxicity of different concentrations of Lindane insecticide affecting behavior, intestinal function and inducing tissue lesions of immature grass carp.
PB  - Dordrecht: Springer Science and Business Media B.V.
T2  - Veterinary Research Communications
T1  - Histological effects of sublethal concentrations of insecticide Lindane on intestinal tissue of grass carp (Ctenopharyngodon idella).
DO  - 10.1007/s11259-021-09818-y
ER  - 

@article{
author = "Vajargah, Mohammad Forouhar and Namin, Javid Imanpour and Mohsenpour, Reza and Yalsuyi, Ahmad Mohammadi and Prokić, Marko and Faggio, Caterina",
year = "2021",
abstract = "This study investigates acute toxicity and histological effects of Lindane insecticide on intestinal tissues of immature grass carp (Ctenopharyngodon idella). For these purposes, 105 fish were exposed to different concentrations of Lindane for 96 h in 5 groups with 3 replications. Samples of intestinal tissues were prepared in both control and exposure groups during and after the toxicity test. Vaculation, necrosis, bleeding and epithelial degeneration were apparent in the intestine tissue of treated individuals. The LC50 96 h of Lindane was 0.788 ppm in the present study, also the results showed besides pesticide-induced lesions, symptoms of abnormal swimming, anxiety, tendency to swim near the surface, and death due to terminal toxicity with mouth widely open. The exposed fish were pale in color, showed oxygen deficit and severe respiratory problems. Overall, our results confirm the toxicity of different concentrations of Lindane insecticide affecting behavior, intestinal function and inducing tissue lesions of immature grass carp.",
publisher = "Dordrecht: Springer Science and Business Media B.V.",
journal = "Veterinary Research Communications",
title = "Histological effects of sublethal concentrations of insecticide Lindane on intestinal tissue of grass carp (Ctenopharyngodon idella).",
doi = "10.1007/s11259-021-09818-y"
}

Vajargah, M. F., Namin, J. I., Mohsenpour, R., Yalsuyi, A. M., Prokić, M.,& Faggio, C.. (2021). Histological effects of sublethal concentrations of insecticide Lindane on intestinal tissue of grass carp (Ctenopharyngodon idella).. in Veterinary Research Communications
Dordrecht: Springer Science and Business Media B.V...
https://doi.org/10.1007/s11259-021-09818-y

Vajargah MF, Namin JI, Mohsenpour R, Yalsuyi AM, Prokić M, Faggio C. Histological effects of sublethal concentrations of insecticide Lindane on intestinal tissue of grass carp (Ctenopharyngodon idella).. in Veterinary Research Communications. 2021;.
doi:10.1007/s11259-021-09818-y .

Vajargah, Mohammad Forouhar, Namin, Javid Imanpour, Mohsenpour, Reza, Yalsuyi, Ahmad Mohammadi, Prokić, Marko, Faggio, Caterina, "Histological effects of sublethal concentrations of insecticide Lindane on intestinal tissue of grass carp (Ctenopharyngodon idella)." in Veterinary Research Communications (2021),
https://doi.org/10.1007/s11259-021-09818-y . .

TY  - JOUR
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Petrović, Tamara
AU  - Ajduković, Maja
AU  - Vučić, Tijana
AU  - Cvijanović, Milena
AU  - Faggio, Caterina
AU  - Prokić, Marko
PY  - 2021
UR  - https://www.mdpi.com/1660-4601/18/18/9653
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8464833
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4483
AB  - Thiourea is an established disruptor of thyroid hormone synthesis and is frequently used as an inhibitor of metamorphosis. The changes caused by thiourea can affect processes associated with the oxidative status of individuals (metabolic rate, the HPI axis, antioxidant system). We investigated the parameters of oxidative stress in crested newt (Triturus spp.) larvae during normal development in late larval stage 62 and newly metamorphosed individuals, and during thiourea-stimulated metamorphosis arrest in individuals exposed to low (0.05%) and high (0.1%) concentrations of thiourea. Both groups of crested newts exposed to thiourea retained their larval characteristics until the end of the experiment. The low activities of antioxidant enzymes and the high lipid peroxidation level pointed to increased oxidative stress in larvae at the beginning of stage 62 as compared to fully metamorphosed individuals. The activities of catalase (CAT) and glutathione-S-transferase (GST) and the concentration of sulfhydryl (SH) groups were significantly lower in larvae reared in aqueous solutions containing thiourea than in newly metamorphosed individuals. The high thiourea concentration (0.1%) affected the antioxidative parameters to the extent that oxidative damage could not be avoided, contrary to a lower concentration. Our results provide a first insight into the physiological adaptations of crested newts during normal development and simulated metamorphosis arrest.
PB  - Basel: MDPI
T2  - International Journal of Environmental Research and Public Health
T1  - Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis.
IS  - 18
VL  - 18
DO  - 10.3390/ijerph18189653
SP  - 9653
ER  - 

@article{
author = "Gavrić, Jelena and Despotović, Svetlana and Gavrilović, Branka and Radovanović, Tijana and Petrović, Tamara and Ajduković, Maja and Vučić, Tijana and Cvijanović, Milena and Faggio, Caterina and Prokić, Marko",
year = "2021",
abstract = "Thiourea is an established disruptor of thyroid hormone synthesis and is frequently used as an inhibitor of metamorphosis. The changes caused by thiourea can affect processes associated with the oxidative status of individuals (metabolic rate, the HPI axis, antioxidant system). We investigated the parameters of oxidative stress in crested newt (Triturus spp.) larvae during normal development in late larval stage 62 and newly metamorphosed individuals, and during thiourea-stimulated metamorphosis arrest in individuals exposed to low (0.05%) and high (0.1%) concentrations of thiourea. Both groups of crested newts exposed to thiourea retained their larval characteristics until the end of the experiment. The low activities of antioxidant enzymes and the high lipid peroxidation level pointed to increased oxidative stress in larvae at the beginning of stage 62 as compared to fully metamorphosed individuals. The activities of catalase (CAT) and glutathione-S-transferase (GST) and the concentration of sulfhydryl (SH) groups were significantly lower in larvae reared in aqueous solutions containing thiourea than in newly metamorphosed individuals. The high thiourea concentration (0.1%) affected the antioxidative parameters to the extent that oxidative damage could not be avoided, contrary to a lower concentration. Our results provide a first insight into the physiological adaptations of crested newts during normal development and simulated metamorphosis arrest.",
publisher = "Basel: MDPI",
journal = "International Journal of Environmental Research and Public Health",
title = "Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis.",
number = "18",
volume = "18",
doi = "10.3390/ijerph18189653",
pages = "9653"
}

Gavrić, J., Despotović, S., Gavrilović, B., Radovanović, T., Petrović, T., Ajduković, M., Vučić, T., Cvijanović, M., Faggio, C.,& Prokić, M.. (2021). Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis.. in International Journal of Environmental Research and Public Health
Basel: MDPI., 18(18), 9653.
https://doi.org/10.3390/ijerph18189653

Gavrić J, Despotović S, Gavrilović B, Radovanović T, Petrović T, Ajduković M, Vučić T, Cvijanović M, Faggio C, Prokić M. Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis.. in International Journal of Environmental Research and Public Health. 2021;18(18):9653.
doi:10.3390/ijerph18189653 .

Gavrić, Jelena, Despotović, Svetlana, Gavrilović, Branka, Radovanović, Tijana, Petrović, Tamara, Ajduković, Maja, Vučić, Tijana, Cvijanović, Milena, Faggio, Caterina, Prokić, Marko, "Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis." in International Journal of Environmental Research and Public Health, 18, no. 18 (2021):9653,
https://doi.org/10.3390/ijerph18189653 . .

TY  - JOUR
AU  - Radovanović, Tijana
AU  - Gavrilović, Branka
AU  - Petrović, Tamara
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Kijanović, Ana
AU  - Mirč, Marko
AU  - Tomašević Kolarov, Nataša
AU  - Faggio, Caterina
AU  - Prokić, Marko
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S1532045621002180
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4484
AB  - Global warming represents a severe threat to existing ecosystems, especially for anuran tadpoles who encounter significant fluctuations in their habitats. Decreasing water levels in permanent and temporary water bodies is a significant risk for larval survival or fitness. On the other hand, the natural environment of amphibians is extremely polluted by various xenobiotics. This study evaluated how pre-exposure of Bombina variegata tadpoles to chronic environmental stress (desiccation) modulates the biochemical response of juvenile individuals to following acute chemical stressor (pesticide deltamethrin). Our results demonstrated that individually applied pesticide changed the thiol and lipid status of the treated juveniles but animals subjected solely to desiccation pressure were more tolerant to free radicals and showed no induction of lipid peroxidation. Comparison of juveniles exposed to deltamethrin revealed that desiccation pretreatment during the larval stage of development modified cellular protection in the juveniles. Higher activities of CAT, GSH-Px and GR were recorded in the pre-exposed group, as well as a lower degree of lipid peroxidation relative to the group that was not pre-exposed to low water stress. Pre-desiccated groups displayed the greatest range of coordination of investigated antioxidant parameters, supported by Pearson's correlations. Activation of the GSH-redox system is a significant marker in juveniles against stress caused by desiccation and a chemical stressor. The stressful environment experienced during tadpole development produced an adaptive reaction to subsequent exposure to another stressor in juveniles. To develop relevant management and conservation strategies, more studies of the interactive effects of environmental and chemical stressors are necessary.
PB  - New York: Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.
VL  - 250
DO  - 10.1016/j.cbpc.2021.109191
SP  - 109191
ER  - 

@article{
author = "Radovanović, Tijana and Gavrilović, Branka and Petrović, Tamara and Despotović, Svetlana and Gavrić, Jelena and Kijanović, Ana and Mirč, Marko and Tomašević Kolarov, Nataša and Faggio, Caterina and Prokić, Marko",
year = "2021",
abstract = "Global warming represents a severe threat to existing ecosystems, especially for anuran tadpoles who encounter significant fluctuations in their habitats. Decreasing water levels in permanent and temporary water bodies is a significant risk for larval survival or fitness. On the other hand, the natural environment of amphibians is extremely polluted by various xenobiotics. This study evaluated how pre-exposure of Bombina variegata tadpoles to chronic environmental stress (desiccation) modulates the biochemical response of juvenile individuals to following acute chemical stressor (pesticide deltamethrin). Our results demonstrated that individually applied pesticide changed the thiol and lipid status of the treated juveniles but animals subjected solely to desiccation pressure were more tolerant to free radicals and showed no induction of lipid peroxidation. Comparison of juveniles exposed to deltamethrin revealed that desiccation pretreatment during the larval stage of development modified cellular protection in the juveniles. Higher activities of CAT, GSH-Px and GR were recorded in the pre-exposed group, as well as a lower degree of lipid peroxidation relative to the group that was not pre-exposed to low water stress. Pre-desiccated groups displayed the greatest range of coordination of investigated antioxidant parameters, supported by Pearson's correlations. Activation of the GSH-redox system is a significant marker in juveniles against stress caused by desiccation and a chemical stressor. The stressful environment experienced during tadpole development produced an adaptive reaction to subsequent exposure to another stressor in juveniles. To develop relevant management and conservation strategies, more studies of the interactive effects of environmental and chemical stressors are necessary.",
publisher = "New York: Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.",
volume = "250",
doi = "10.1016/j.cbpc.2021.109191",
pages = "109191"
}

Radovanović, T., Gavrilović, B., Petrović, T., Despotović, S., Gavrić, J., Kijanović, A., Mirč, M., Tomašević Kolarov, N., Faggio, C.,& Prokić, M.. (2021). Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
New York: Elsevier Inc.., 250, 109191.
https://doi.org/10.1016/j.cbpc.2021.109191

Radovanović T, Gavrilović B, Petrović T, Despotović S, Gavrić J, Kijanović A, Mirč M, Tomašević Kolarov N, Faggio C, Prokić M. Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2021;250:109191.
doi:10.1016/j.cbpc.2021.109191 .

Radovanović, Tijana, Gavrilović, Branka, Petrović, Tamara, Despotović, Svetlana, Gavrić, Jelena, Kijanović, Ana, Mirč, Marko, Tomašević Kolarov, Nataša, Faggio, Caterina, Prokić, Marko, "Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles." in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 250 (2021):109191,
https://doi.org/10.1016/j.cbpc.2021.109191 . .

TY  - JOUR
AU  - Radovanović, Tijana
AU  - Gavrilović, Branka
AU  - Petrović, Tamara
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Kijanović, Ana
AU  - Mirč, Marko
AU  - Tomašević Kolarov, Nataša
AU  - Faggio, Caterina
AU  - Prokić, Marko
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S1532045621002180
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4484
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4497
AB  - Global warming represents a severe threat to existing ecosystems, especially for anuran tadpoles who encounter significant fluctuations in their habitats. Decreasing water levels in permanent and temporary water bodies is a significant risk for larval survival or fitness. On the other hand, the natural environment of amphibians is extremely polluted by various xenobiotics. This study evaluated how pre-exposure of Bombina variegata tadpoles to chronic environmental stress (desiccation) modulates the biochemical response of juvenile individuals to following acute chemical stressor (pesticide deltamethrin). Our results demonstrated that individually applied pesticide changed the thiol and lipid status of the treated juveniles but animals subjected solely to desiccation pressure were more tolerant to free radicals and showed no induction of lipid peroxidation. Comparison of juveniles exposed to deltamethrin revealed that desiccation pretreatment during the larval stage of development modified cellular protection in the juveniles. Higher activities of CAT, GSH-Px and GR were recorded in the pre-exposed group, as well as a lower degree of lipid peroxidation relative to the group that was not pre-exposed to low water stress. Pre-desiccated groups displayed the greatest range of coordination of investigated antioxidant parameters, supported by Pearson's correlations. Activation of the GSH-redox system is a significant marker in juveniles against stress caused by desiccation and a chemical stressor. The stressful environment experienced during tadpole development produced an adaptive reaction to subsequent exposure to another stressor in juveniles. To develop relevant management and conservation strategies, more studies of the interactive effects of environmental and chemical stressors are necessary.
PB  - New York: Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.
VL  - 250
DO  - 10.1016/j.cbpc.2021.109191
SP  - 109191
ER  - 

@article{
author = "Radovanović, Tijana and Gavrilović, Branka and Petrović, Tamara and Despotović, Svetlana and Gavrić, Jelena and Kijanović, Ana and Mirč, Marko and Tomašević Kolarov, Nataša and Faggio, Caterina and Prokić, Marko",
year = "2021",
abstract = "Global warming represents a severe threat to existing ecosystems, especially for anuran tadpoles who encounter significant fluctuations in their habitats. Decreasing water levels in permanent and temporary water bodies is a significant risk for larval survival or fitness. On the other hand, the natural environment of amphibians is extremely polluted by various xenobiotics. This study evaluated how pre-exposure of Bombina variegata tadpoles to chronic environmental stress (desiccation) modulates the biochemical response of juvenile individuals to following acute chemical stressor (pesticide deltamethrin). Our results demonstrated that individually applied pesticide changed the thiol and lipid status of the treated juveniles but animals subjected solely to desiccation pressure were more tolerant to free radicals and showed no induction of lipid peroxidation. Comparison of juveniles exposed to deltamethrin revealed that desiccation pretreatment during the larval stage of development modified cellular protection in the juveniles. Higher activities of CAT, GSH-Px and GR were recorded in the pre-exposed group, as well as a lower degree of lipid peroxidation relative to the group that was not pre-exposed to low water stress. Pre-desiccated groups displayed the greatest range of coordination of investigated antioxidant parameters, supported by Pearson's correlations. Activation of the GSH-redox system is a significant marker in juveniles against stress caused by desiccation and a chemical stressor. The stressful environment experienced during tadpole development produced an adaptive reaction to subsequent exposure to another stressor in juveniles. To develop relevant management and conservation strategies, more studies of the interactive effects of environmental and chemical stressors are necessary.",
publisher = "New York: Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.",
volume = "250",
doi = "10.1016/j.cbpc.2021.109191",
pages = "109191"
}

Radovanović, T., Gavrilović, B., Petrović, T., Despotović, S., Gavrić, J., Kijanović, A., Mirč, M., Tomašević Kolarov, N., Faggio, C.,& Prokić, M.. (2021). Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
New York: Elsevier Inc.., 250, 109191.
https://doi.org/10.1016/j.cbpc.2021.109191

Radovanović T, Gavrilović B, Petrović T, Despotović S, Gavrić J, Kijanović A, Mirč M, Tomašević Kolarov N, Faggio C, Prokić M. Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2021;250:109191.
doi:10.1016/j.cbpc.2021.109191 .

Radovanović, Tijana, Gavrilović, Branka, Petrović, Tamara, Despotović, Svetlana, Gavrić, Jelena, Kijanović, Ana, Mirč, Marko, Tomašević Kolarov, Nataša, Faggio, Caterina, Prokić, Marko, "Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles." in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 250 (2021):109191,
https://doi.org/10.1016/j.cbpc.2021.109191 . .

TY  - JOUR
AU  - Yalsuyi, Ahmad Mohamadi
AU  - Vajargah, Mohammad Forouhar
AU  - Hajimoradloo, Abdolmajid
AU  - Galangash, Mohsen Mohammadi
AU  - Prokić, Marko
AU  - Faggio, Caterina
PY  - 2021
UR  - https://www.mdpi.com/2306-7381/8/10/218
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8540590
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4633
AB  - Pesticides can induce changes in behavior and reduce the survival chance of aquatic organisms. In this study, the toxic effects of glyphosate suspension (Glyphosate Aria 41% SL, Tehran Iran) on behavior and tissues of common carp (Cyprinus carpio) were assessed. For this purpose, a 96 h LC50 of glyphosate suspension (68.788 mL·L-1) was used in the toxicity test. All individuals were divided into control and treatment groups with four replicates. Exposure operations were performed under two conditions: increasing concentration of suspension from 0 to 68.788 mL·L-1; then, decreasing to the first level. The swimming pattern was recorded by digital cameras during the test and tissue samples were collected at the end of the test. There were significant differences between the swimming pattern of treated individuals and control ones during both steps. The sublethal concentration of glyphosate led to hypertrophy, hyperplasia and hyperemia in the gill of fish. However, changes were obvious only after sampling. The exposed fish also displayed clinical signs such as darkening of the skin and increasing movement of the operculum. Moreover, glyphosate suspension affected swimming patterns of fish suggest that the swimming behavior test can indicate the potential toxicity of environmental pollutants and be used as a noninvasive, useful method for managing environmental changes and assessing fish health conditions by video monitoring.
PB  - Basel: MDPI
T2  - Veterinary Sciences
T1  - Evaluation of Behavioral Changes and Tissue Damages in Common Carp (Cyprinus carpio) after Exposure to the Herbicide Glyphosate.
IS  - 10
VL  - 8
DO  - 10.3390/vetsci8100218
SP  - 218
ER  - 

@article{
author = "Yalsuyi, Ahmad Mohamadi and Vajargah, Mohammad Forouhar and Hajimoradloo, Abdolmajid and Galangash, Mohsen Mohammadi and Prokić, Marko and Faggio, Caterina",
year = "2021",
abstract = "Pesticides can induce changes in behavior and reduce the survival chance of aquatic organisms. In this study, the toxic effects of glyphosate suspension (Glyphosate Aria 41% SL, Tehran Iran) on behavior and tissues of common carp (Cyprinus carpio) were assessed. For this purpose, a 96 h LC50 of glyphosate suspension (68.788 mL·L-1) was used in the toxicity test. All individuals were divided into control and treatment groups with four replicates. Exposure operations were performed under two conditions: increasing concentration of suspension from 0 to 68.788 mL·L-1; then, decreasing to the first level. The swimming pattern was recorded by digital cameras during the test and tissue samples were collected at the end of the test. There were significant differences between the swimming pattern of treated individuals and control ones during both steps. The sublethal concentration of glyphosate led to hypertrophy, hyperplasia and hyperemia in the gill of fish. However, changes were obvious only after sampling. The exposed fish also displayed clinical signs such as darkening of the skin and increasing movement of the operculum. Moreover, glyphosate suspension affected swimming patterns of fish suggest that the swimming behavior test can indicate the potential toxicity of environmental pollutants and be used as a noninvasive, useful method for managing environmental changes and assessing fish health conditions by video monitoring.",
publisher = "Basel: MDPI",
journal = "Veterinary Sciences",
title = "Evaluation of Behavioral Changes and Tissue Damages in Common Carp (Cyprinus carpio) after Exposure to the Herbicide Glyphosate.",
number = "10",
volume = "8",
doi = "10.3390/vetsci8100218",
pages = "218"
}

Yalsuyi, A. M., Vajargah, M. F., Hajimoradloo, A., Galangash, M. M., Prokić, M.,& Faggio, C.. (2021). Evaluation of Behavioral Changes and Tissue Damages in Common Carp (Cyprinus carpio) after Exposure to the Herbicide Glyphosate.. in Veterinary Sciences
Basel: MDPI., 8(10), 218.
https://doi.org/10.3390/vetsci8100218

Yalsuyi AM, Vajargah MF, Hajimoradloo A, Galangash MM, Prokić M, Faggio C. Evaluation of Behavioral Changes and Tissue Damages in Common Carp (Cyprinus carpio) after Exposure to the Herbicide Glyphosate.. in Veterinary Sciences. 2021;8(10):218.
doi:10.3390/vetsci8100218 .

Yalsuyi, Ahmad Mohamadi, Vajargah, Mohammad Forouhar, Hajimoradloo, Abdolmajid, Galangash, Mohsen Mohammadi, Prokić, Marko, Faggio, Caterina, "Evaluation of Behavioral Changes and Tissue Damages in Common Carp (Cyprinus carpio) after Exposure to the Herbicide Glyphosate." in Veterinary Sciences, 8, no. 10 (2021):218,
https://doi.org/10.3390/vetsci8100218 . .

TY  - JOUR
AU  - Mohamadi Yalsuyi, Ahmad
AU  - Forouhar Vajargah, Mohammad
AU  - Hajimoradloo, Abdolmajid
AU  - Mohammadi Galangash, Mohsen
AU  - Prokić, Marko
AU  - Faggio, Caterina
PY  - 2021
UR  - https://link.springer.com/10.1007/s11259-021-09862-8
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4691
AB  - Industrial chemical solutions are widely used as a method to disinfection of aquaculture water and environments. The aim of the present study was to evaluate the toxicity effect of Betadine (10% solution of povidone-iodine) as a disinfectant solution on the survival and gill tissue of Oranda goldfish (Carassius auratus). For these purposes, 225 fingerling Oranda goldfish with an average weight 5 ± 0.67 g were divided into 15 groups with 3 replications. Fish were exposed to series of concentrations (0, 10, 20, 40, 60, 80, 90, 100, 120, 140, 160, 180, 200, 220 and 240 mg/L) of Betadine for 96 h. The mortality of fish and the samples of gill were observed at 6, 12, 18, 24, 48, 72 and 96 h after exposure. The results of the present study showed that the half-life of Betadine was less than 24 h and mortality were not record after 24 h. The highest mortality rate was at 240 mg/L, and LC50 24 h of Betadine was 158.800 mg/L. Histopathological results showed that lethal concentrations of Betadine lead to hyperemia, hypertrophy, hyperplasia and adhesion of secondary lamellar of the gill. Moreover, fish that were exposed to these concentrations displayed clinical signs such as anxiety, darkening of the skin. Overall results showed that Betadine have short half-life in the aquatic environment and are toxic to fish at very high concentrations, therefore it can be considered as practically non-toxic and useful for disinfection of the aquatic environment.
PB  - Dordrecht: Springer Science and Business Media B.V.
T2  - Veterinary Research Communications
T1  - Can Betadine (10% povidone-iodine solution) act on the survival rate and gill tissue structure of Oranda goldfish (Carassius auratus)?
DO  - 10.1007/s11259-021-09862-8
ER  - 

@article{
author = "Mohamadi Yalsuyi, Ahmad and Forouhar Vajargah, Mohammad and Hajimoradloo, Abdolmajid and Mohammadi Galangash, Mohsen and Prokić, Marko and Faggio, Caterina",
year = "2021",
abstract = "Industrial chemical solutions are widely used as a method to disinfection of aquaculture water and environments. The aim of the present study was to evaluate the toxicity effect of Betadine (10% solution of povidone-iodine) as a disinfectant solution on the survival and gill tissue of Oranda goldfish (Carassius auratus). For these purposes, 225 fingerling Oranda goldfish with an average weight 5 ± 0.67 g were divided into 15 groups with 3 replications. Fish were exposed to series of concentrations (0, 10, 20, 40, 60, 80, 90, 100, 120, 140, 160, 180, 200, 220 and 240 mg/L) of Betadine for 96 h. The mortality of fish and the samples of gill were observed at 6, 12, 18, 24, 48, 72 and 96 h after exposure. The results of the present study showed that the half-life of Betadine was less than 24 h and mortality were not record after 24 h. The highest mortality rate was at 240 mg/L, and LC50 24 h of Betadine was 158.800 mg/L. Histopathological results showed that lethal concentrations of Betadine lead to hyperemia, hypertrophy, hyperplasia and adhesion of secondary lamellar of the gill. Moreover, fish that were exposed to these concentrations displayed clinical signs such as anxiety, darkening of the skin. Overall results showed that Betadine have short half-life in the aquatic environment and are toxic to fish at very high concentrations, therefore it can be considered as practically non-toxic and useful for disinfection of the aquatic environment.",
publisher = "Dordrecht: Springer Science and Business Media B.V.",
journal = "Veterinary Research Communications",
title = "Can Betadine (10% povidone-iodine solution) act on the survival rate and gill tissue structure of Oranda goldfish (Carassius auratus)?",
doi = "10.1007/s11259-021-09862-8"
}

Mohamadi Yalsuyi, A., Forouhar Vajargah, M., Hajimoradloo, A., Mohammadi Galangash, M., Prokić, M.,& Faggio, C.. (2021). Can Betadine (10% povidone-iodine solution) act on the survival rate and gill tissue structure of Oranda goldfish (Carassius auratus)?. in Veterinary Research Communications
Dordrecht: Springer Science and Business Media B.V...
https://doi.org/10.1007/s11259-021-09862-8

Mohamadi Yalsuyi A, Forouhar Vajargah M, Hajimoradloo A, Mohammadi Galangash M, Prokić M, Faggio C. Can Betadine (10% povidone-iodine solution) act on the survival rate and gill tissue structure of Oranda goldfish (Carassius auratus)?. in Veterinary Research Communications. 2021;.
doi:10.1007/s11259-021-09862-8 .

Mohamadi Yalsuyi, Ahmad, Forouhar Vajargah, Mohammad, Hajimoradloo, Abdolmajid, Mohammadi Galangash, Mohsen, Prokić, Marko, Faggio, Caterina, "Can Betadine (10% povidone-iodine solution) act on the survival rate and gill tissue structure of Oranda goldfish (Carassius auratus)?" in Veterinary Research Communications (2021),
https://doi.org/10.1007/s11259-021-09862-8 . .

TY  - JOUR
AU  - Prokić, Marko
AU  - Petrović, Tamara
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Kijanović, Ana
AU  - Tomašević Kolarov, Nataša
AU  - Vukov, Tanja
AU  - Radovanović, Tijana
PY  - 2021
UR  - https://www.frontiersin.org/articles/10.3389/fphys.2021.783288/full
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8674722
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4757
AB  - Amphibians are sensitive to deteriorating environmental conditions, especially during transition to a terrestrial environment which is full of uncertainties. Harsh conditions, such as desiccation during earlier stages, affect different larval traits with possible carry-over effects on juvenile and adult life histories. The first consequences of the effects can be seen in juveniles in the challenges to find food and the ability to survive without it in a terrestrial habitat. Body size and the internal energy reserves acquired during the larval phase play an important role in this period. Herein, we tested how different water regimes (low water availability, desiccation and constant high-water availability) during larval development reflect on the oxidative status and ability of yellow belly toad (Bombina variegata) juveniles to endure short-term fasting. The desiccation regime significantly reduced the body size of metamorphs. The same was observed after 2 weeks of fasting, while the feeding treatment reduced differences mostly in the body mass of individuals from different water regimes. This was the result of a greater gain in mass in juveniles pre-exposed to desiccation. Pre-exposure to desiccation also modified the parameters of the antioxidant system (AOS) under feeding conditions, leading to higher values of superoxide dismutase, glutathione reductase and glutathione S-transferase, glutathione and sulfhydryl group concentrations, and lower glutathione peroxidase in comparison to juveniles reared under constant water. The increase in the AOS of juveniles can be considered as a physiological carry-over effect of desiccation, probably as the result of compensatory growth and/or earlier exposure to chronic stress. However, water levels during larval development did not exert significant effects on the oxidative status of juveniles subjected to food unavailability. Fasting juveniles, both control and desiccated, were exposed to oxidative stress, significantly higher lipid peroxide concentrations, lower superoxide dismutase, glutathione peroxidase, glutathione S-transferase, glutathione and sulfhydryl group values in comparison to feeding individuals. The lack of food in juvenile anurans activated the AOS response in the same manner, regardless of body size and stress pre-exposure, suggesting that the generally accepted hypothesis about the influence of metamorphic body size on the fitness of the postmetamorphic stage should be tested further.
T2  - Frontiers in Physiology
T1  - Carry-Over Effects of Desiccation Stress on the Oxidative Status of Fasting Anuran Juveniles.
VL  - 12
DO  - 10.3389/fphys.2021.783288
SP  - 783288
ER  - 

@article{
author = "Prokić, Marko and Petrović, Tamara and Gavrilović, Branka and Despotović, Svetlana and Gavrić, Jelena and Kijanović, Ana and Tomašević Kolarov, Nataša and Vukov, Tanja and Radovanović, Tijana",
year = "2021",
abstract = "Amphibians are sensitive to deteriorating environmental conditions, especially during transition to a terrestrial environment which is full of uncertainties. Harsh conditions, such as desiccation during earlier stages, affect different larval traits with possible carry-over effects on juvenile and adult life histories. The first consequences of the effects can be seen in juveniles in the challenges to find food and the ability to survive without it in a terrestrial habitat. Body size and the internal energy reserves acquired during the larval phase play an important role in this period. Herein, we tested how different water regimes (low water availability, desiccation and constant high-water availability) during larval development reflect on the oxidative status and ability of yellow belly toad (Bombina variegata) juveniles to endure short-term fasting. The desiccation regime significantly reduced the body size of metamorphs. The same was observed after 2 weeks of fasting, while the feeding treatment reduced differences mostly in the body mass of individuals from different water regimes. This was the result of a greater gain in mass in juveniles pre-exposed to desiccation. Pre-exposure to desiccation also modified the parameters of the antioxidant system (AOS) under feeding conditions, leading to higher values of superoxide dismutase, glutathione reductase and glutathione S-transferase, glutathione and sulfhydryl group concentrations, and lower glutathione peroxidase in comparison to juveniles reared under constant water. The increase in the AOS of juveniles can be considered as a physiological carry-over effect of desiccation, probably as the result of compensatory growth and/or earlier exposure to chronic stress. However, water levels during larval development did not exert significant effects on the oxidative status of juveniles subjected to food unavailability. Fasting juveniles, both control and desiccated, were exposed to oxidative stress, significantly higher lipid peroxide concentrations, lower superoxide dismutase, glutathione peroxidase, glutathione S-transferase, glutathione and sulfhydryl group values in comparison to feeding individuals. The lack of food in juvenile anurans activated the AOS response in the same manner, regardless of body size and stress pre-exposure, suggesting that the generally accepted hypothesis about the influence of metamorphic body size on the fitness of the postmetamorphic stage should be tested further.",
journal = "Frontiers in Physiology",
title = "Carry-Over Effects of Desiccation Stress on the Oxidative Status of Fasting Anuran Juveniles.",
volume = "12",
doi = "10.3389/fphys.2021.783288",
pages = "783288"
}

Prokić, M., Petrović, T., Gavrilović, B., Despotović, S., Gavrić, J., Kijanović, A., Tomašević Kolarov, N., Vukov, T.,& Radovanović, T.. (2021). Carry-Over Effects of Desiccation Stress on the Oxidative Status of Fasting Anuran Juveniles.. in Frontiers in Physiology, 12, 783288.
https://doi.org/10.3389/fphys.2021.783288

Prokić M, Petrović T, Gavrilović B, Despotović S, Gavrić J, Kijanović A, Tomašević Kolarov N, Vukov T, Radovanović T. Carry-Over Effects of Desiccation Stress on the Oxidative Status of Fasting Anuran Juveniles.. in Frontiers in Physiology. 2021;12:783288.
doi:10.3389/fphys.2021.783288 .

Prokić, Marko, Petrović, Tamara, Gavrilović, Branka, Despotović, Svetlana, Gavrić, Jelena, Kijanović, Ana, Tomašević Kolarov, Nataša, Vukov, Tanja, Radovanović, Tijana, "Carry-Over Effects of Desiccation Stress on the Oxidative Status of Fasting Anuran Juveniles." in Frontiers in Physiology, 12 (2021):783288,
https://doi.org/10.3389/fphys.2021.783288 . .

TY  - JOUR
AU  - Prokić, Marko
AU  - Petrović, Tamara
AU  - Despotović, Svetlana
AU  - Vučić, Tijana
AU  - Gavrić, Jelena
AU  - Radovanović, Tijana
AU  - Gavrilović, Branka
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S1095643320301720
UR  - https://radar.ibiss.bg.ac.rs/123456789/3902
UR  - https://radar.ibiss.bg.ac.rs/123456789/3903
AB  - In nature, animals often face periods without food caused by seasonal fluctuations and/or prey scarcity. An organism's physiological response to imposed energetic limitations is followed by changes in mitochondrial functioning (adjustment of energy metabolism) and a reduction of non-essential processes. However, this energy-saving strategy can have its costs. In this study, we examined oxidative stress as one of the possible physiological costs of short-term, two-week-long food deprivation on developing amphibian larvae of the crested newts Triturus macedonicus and Triturus ivanbureschi and their hybrids. We investigated whether this exogenous factor additionally affected the oxidative status (fitness-related trait) of hybrid individuals. The fasting treatment led to lower growth and a lower body mass and body condition index of individuals. The results revealed that the antioxidant system (AOS) of food-deprived larvae could not cope in a proper manner with reactive oxygen species production under limited energy availability, leading to higher lipid oxidative damage. The lowest AOS response was observed for H2O2 scavenging parameters (catalase, glutathione peroxidase, and total glutathione), which together with the elevated activity of superoxide dismutase suggested increased H2O2 concentrations. Comparison between parental species and their hybrids showed that hybrid individuals suffered greater oxidative damage (as demonstrated by higher concentrations of lipid peroxides), indicating that they were more susceptible to fasting-induced oxidative stress. Overall, this study illustrates that: (i) an oxidative event is one of the costs amphibian larvae face during short-term periods of fasting, (ii) hybrids are less capable of dealing with this stressful condition, which can lower their chances of survival in a changing environment.
PB  - Pergamon
T2  - Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
T1  - The effect of short-term fasting on the oxidative status of larvae of crested newt species and their hybrids
VL  - 251
DO  - 10.1016/j.cbpa.2020.110819
SP  - 110819
ER  - 

@article{
author = "Prokić, Marko and Petrović, Tamara and Despotović, Svetlana and Vučić, Tijana and Gavrić, Jelena and Radovanović, Tijana and Gavrilović, Branka",
year = "2021",
abstract = "In nature, animals often face periods without food caused by seasonal fluctuations and/or prey scarcity. An organism's physiological response to imposed energetic limitations is followed by changes in mitochondrial functioning (adjustment of energy metabolism) and a reduction of non-essential processes. However, this energy-saving strategy can have its costs. In this study, we examined oxidative stress as one of the possible physiological costs of short-term, two-week-long food deprivation on developing amphibian larvae of the crested newts Triturus macedonicus and Triturus ivanbureschi and their hybrids. We investigated whether this exogenous factor additionally affected the oxidative status (fitness-related trait) of hybrid individuals. The fasting treatment led to lower growth and a lower body mass and body condition index of individuals. The results revealed that the antioxidant system (AOS) of food-deprived larvae could not cope in a proper manner with reactive oxygen species production under limited energy availability, leading to higher lipid oxidative damage. The lowest AOS response was observed for H2O2 scavenging parameters (catalase, glutathione peroxidase, and total glutathione), which together with the elevated activity of superoxide dismutase suggested increased H2O2 concentrations. Comparison between parental species and their hybrids showed that hybrid individuals suffered greater oxidative damage (as demonstrated by higher concentrations of lipid peroxides), indicating that they were more susceptible to fasting-induced oxidative stress. Overall, this study illustrates that: (i) an oxidative event is one of the costs amphibian larvae face during short-term periods of fasting, (ii) hybrids are less capable of dealing with this stressful condition, which can lower their chances of survival in a changing environment.",
publisher = "Pergamon",
journal = "Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology",
title = "The effect of short-term fasting on the oxidative status of larvae of crested newt species and their hybrids",
volume = "251",
doi = "10.1016/j.cbpa.2020.110819",
pages = "110819"
}

Prokić, M., Petrović, T., Despotović, S., Vučić, T., Gavrić, J., Radovanović, T.,& Gavrilović, B.. (2021). The effect of short-term fasting on the oxidative status of larvae of crested newt species and their hybrids. in Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
Pergamon., 251, 110819.
https://doi.org/10.1016/j.cbpa.2020.110819

Prokić M, Petrović T, Despotović S, Vučić T, Gavrić J, Radovanović T, Gavrilović B. The effect of short-term fasting on the oxidative status of larvae of crested newt species and their hybrids. in Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. 2021;251:110819.
doi:10.1016/j.cbpa.2020.110819 .

Prokić, Marko, Petrović, Tamara, Despotović, Svetlana, Vučić, Tijana, Gavrić, Jelena, Radovanović, Tijana, Gavrilović, Branka, "The effect of short-term fasting on the oxidative status of larvae of crested newt species and their hybrids" in Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 251 (2021):110819,
https://doi.org/10.1016/j.cbpa.2020.110819 . .