@conference{
author = "Marinković, Jelena and Marković, Tatjana and Nikolić, Biljana and Ćirić, Ana and Soković, Marina and Marković, Dejan",
year = "2021",
abstract = "Essential oils (EOs) are potent antibacterial agents that have numerous applications in different fields, such
as traditional medicine, pharmacy and cosmetics, food and beverage industry. However, their use in
dentistry is limited and not adequately explored. In order to investigate the potential to expand their
applications in dentistry, we screened for antibacterial and antibiofilm activity of selected EOs toward
Streptococcus salivarius dental isolates. Selected EOs were originated from Pelargonium roseum,
Pelargonium graveolens, Leptospermum petersonii and Eucalyptus citriodora. In order to take insight into
the variation in sensitivity among bacterial strains of different origin, both isolates from symptomatic and
asymptomatic root canal infections, as well as the reference S. salivarius ATCC 9222 strain, were involved
in this study. EOs were chemically characterized by GC/MS-FID analysis. Microdilution assay, was used
to determine minimal inhibitory concentrations (MICs). Crystal violet (CV) assay was provided to explore
the potential to disturb biofilm formation. Statistical analysis performed by One Way ANOVA was used to
compare sensitivity between tested strains.
Chemical characterization revealed that all EOs were abundant in oxygenated monoterpenes (76.8 – 92.4%
composition). Furthermore, the content of sesquiterpene hydrocarbons was relatively high in P. roseum and
P. graveolens EOs (12.3% and 16.7%, respectively). The major constituents of Pelargonium oils were
citronellol, geraniol, citronellyl formate and geranyl formate, contributing together with 71.3% and 57.1%
of total P. roseum and P. graveolens EOs, respectively. EO of L. petersonii was abundant with following
dominant constituents: geranial, neral, citronellal, citronellol and isopulegol (accounting for 81.2% of the
total EO). Some of these constituents, namely citronellal, citronellol and isopulegol, were also dominant in
E. citriodora oil, constituting 84.8% of its composition. MIC assay demonstrated that referent strain was
the most sensitive, while the strains isolated from symptomatic infections were highly resistant. Considering
all tested strains, the comparison of EOs antibacterial activity pointed out the following order, calculated in
mg mL-1
: E. citriodora (MIC values 0.08±0.03 – 5.42±1.71) > L. petersonii (MIC values 0.53±0.00 –
4.74±0.64) > P. graveolens (MIC values 0.36±0.15 – 5.54±0.00) > P. roseum (MIC values 1.50±0.68 –
13.77±0.69). The inhibition of biofilm formation by EOs was also strain specific; clinical isolates were less
sensitive than the reference strain (the ranges of biofilm inhibition were 24%-75% and 84%-89%,
respectively). Similarly as in the microdilution assay, the E. citriodora EO was the most potent biofilm
inhibitor.
Taking into account all obtained results, Eucalyptus citriodora EO proved promising antibacterial and
antibiofilm activity and could be recommended for further investigation as alternative antimicrobial in
dental practice.",
publisher = "Bragança, Portugal: Instituto Politécnico de Bragança (IPB)",
journal = "1st Natural products application: Health, Cosmetic and Food: book of abstracts",
title = "New insights into anti-streptococcal potential of selected essential oils relevant to dentistry",
pages = "131",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4378"
}
