TY  - JOUR
AU  - Babota, Mihai
AU  - Frumuzachi, Oleg
AU  - Nicolescu, Alexandru
AU  - Dias, Maria Inês
AU  - Pinela, José
AU  - Barros, Lillian
AU  - Añibarro-Ortega, Mikel
AU  - Stojković, Dejan
AU  - Carević, Tamara
AU  - Mocan, Andrei
AU  - López, Víctor
AU  - Crișan, Gianina
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5475
AB  - Wild thyme aerial parts (Serpylli herba) are recognized as a valuable herbal product with antioxidant, anti-inflammatory, and antibacterial effects. Although pharmacopoeial regulations allow its collection exclusively from Thymus serpyllum, substitution with other species is frequent in current practice. This study analyzed the phenolic composition, antioxidant, and enzyme-inhibitory and antimicrobial activity of the hydroethanolic extracts obtained from five Romanian wild thyme species (Thymus alpestris, T. glabrescens, T. panonicus, T. pulcherimus and T. pulegioides). The analysis of individual phenolic constituents was performed through LC-ESI-DAD/MS2, while for the in vitro evaluation of antioxidant potential, TEAC, FRAP, DPPH, TBARS and OxHLIA assays were employed. The anti-enzymatic potential was tested in vitro against tyrosinase, α-glucosidase and acetylcholinesterase. High rosmarinic acid contents were quantified in all species (20.06 ± 0.32–80.49 ± 0.001 mg/g dry extract); phenolic acids derivatives (including salvianolic acids) were confirmed as the principal metabolites of T. alpestris and T. glabrescens, while eriodictyol-O-di-hexoside was found exclusively in T. alpestris. All species showed strong antioxidant potential and moderate anti-enzymatic effect against α-glucosidase and acetylcholinesterase, showing no anti-tyrosinase activity. This is the first detailed report on the chemical and biological profile of T. alpestris collected from Romanian spontaneous flora.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Thymus Species from Romanian Spontaneous Flora as Promising Source of Phenolic Secondary Metabolites with Health-Related Benefits
IS  - 2
VL  - 12
DO  - 10.3390/antiox12020390
SP  - 390
ER  - 

@article{
author = "Babota, Mihai and Frumuzachi, Oleg and Nicolescu, Alexandru and Dias, Maria Inês and Pinela, José and Barros, Lillian and Añibarro-Ortega, Mikel and Stojković, Dejan and Carević, Tamara and Mocan, Andrei and López, Víctor and Crișan, Gianina",
year = "2023",
abstract = "Wild thyme aerial parts (Serpylli herba) are recognized as a valuable herbal product with antioxidant, anti-inflammatory, and antibacterial effects. Although pharmacopoeial regulations allow its collection exclusively from Thymus serpyllum, substitution with other species is frequent in current practice. This study analyzed the phenolic composition, antioxidant, and enzyme-inhibitory and antimicrobial activity of the hydroethanolic extracts obtained from five Romanian wild thyme species (Thymus alpestris, T. glabrescens, T. panonicus, T. pulcherimus and T. pulegioides). The analysis of individual phenolic constituents was performed through LC-ESI-DAD/MS2, while for the in vitro evaluation of antioxidant potential, TEAC, FRAP, DPPH, TBARS and OxHLIA assays were employed. The anti-enzymatic potential was tested in vitro against tyrosinase, α-glucosidase and acetylcholinesterase. High rosmarinic acid contents were quantified in all species (20.06 ± 0.32–80.49 ± 0.001 mg/g dry extract); phenolic acids derivatives (including salvianolic acids) were confirmed as the principal metabolites of T. alpestris and T. glabrescens, while eriodictyol-O-di-hexoside was found exclusively in T. alpestris. All species showed strong antioxidant potential and moderate anti-enzymatic effect against α-glucosidase and acetylcholinesterase, showing no anti-tyrosinase activity. This is the first detailed report on the chemical and biological profile of T. alpestris collected from Romanian spontaneous flora.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Thymus Species from Romanian Spontaneous Flora as Promising Source of Phenolic Secondary Metabolites with Health-Related Benefits",
number = "2",
volume = "12",
doi = "10.3390/antiox12020390",
pages = "390"
}

Babota, M., Frumuzachi, O., Nicolescu, A., Dias, M. I., Pinela, J., Barros, L., Añibarro-Ortega, M., Stojković, D., Carević, T., Mocan, A., López, V.,& Crișan, G.. (2023). Thymus Species from Romanian Spontaneous Flora as Promising Source of Phenolic Secondary Metabolites with Health-Related Benefits. in Antioxidants
Basel: MDPI., 12(2), 390.
https://doi.org/10.3390/antiox12020390

Babota M, Frumuzachi O, Nicolescu A, Dias MI, Pinela J, Barros L, Añibarro-Ortega M, Stojković D, Carević T, Mocan A, López V, Crișan G. Thymus Species from Romanian Spontaneous Flora as Promising Source of Phenolic Secondary Metabolites with Health-Related Benefits. in Antioxidants. 2023;12(2):390.
doi:10.3390/antiox12020390 .

Babota, Mihai, Frumuzachi, Oleg, Nicolescu, Alexandru, Dias, Maria Inês, Pinela, José, Barros, Lillian, Añibarro-Ortega, Mikel, Stojković, Dejan, Carević, Tamara, Mocan, Andrei, López, Víctor, Crișan, Gianina, "Thymus Species from Romanian Spontaneous Flora as Promising Source of Phenolic Secondary Metabolites with Health-Related Benefits" in Antioxidants, 12, no. 2 (2023):390,
https://doi.org/10.3390/antiox12020390 . .

TY  - JOUR
AU  - Othman, Souha
AU  - Añibarro-Ortega, Mikel
AU  - Dias, Maria Inês
AU  - Ćirić, Ana
AU  - Mandim, Filipa
AU  - Soković, Marina
AU  - Ferreira, Isabel C F R
AU  - Pinela, José
AU  - Barros, Lillian
PY  - 2022
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9587281
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5175
AB  - Quince (Cydonia oblonga Mill.) is an astringent fruit widely processed into marmalade and other sweets through processes that discard the peel as a by-product. Therefore, this study was performed to characterize the quince peel composition in nutrients and phytochemicals and evaluate its in vitro biological activity, following a "zero waste" approach. The quince peel dry powder was particularly rich in fiber (20.2 g/100 g), fructose (34 g/100 g), malic acid (7.2 g/100 g), and potassium (692 mg/100 g). Extracts prepared by dynamic hydroethanolic maceration and hot water extraction yielded 4.70 and 4.27 mg/g of phenolic compounds, respectively, with a prevalence of flavan-3-ols. The hydroethanolic extract was the most effective in inhibiting lipid peroxidation and oxidative hemolysis, and also presented better antimicrobial effects against foodborne pathogens, which agreed with the highest flavan-3-ol contents. The extracts were better than control synthetic food additives against some tested fungal and bacterial strains. On the other hand, no ability to inhibit nitric oxide production or toxicity to the tumor and non-tumor cell lines was observed. Furthermore, the solid residues remaining after extraction contained 35-37 g/100 g of fiber. Overall, quince peel can be upcycled into fiber-rich and bioactive ingredients to endow the value chain with natural food fortifiers, preservatives, and health promoters.
PB  - Elsevier Ltd.
T2  - Heliyon
T1  - Valorization of quince peel into functional food ingredients: A path towards "zero waste" and sustainable food systems
IS  - 10
VL  - 8
DO  - 10.1016/j.heliyon.2022.e11042
SP  - e11042
ER  - 

@article{
author = "Othman, Souha and Añibarro-Ortega, Mikel and Dias, Maria Inês and Ćirić, Ana and Mandim, Filipa and Soković, Marina and Ferreira, Isabel C F R and Pinela, José and Barros, Lillian",
year = "2022",
abstract = "Quince (Cydonia oblonga Mill.) is an astringent fruit widely processed into marmalade and other sweets through processes that discard the peel as a by-product. Therefore, this study was performed to characterize the quince peel composition in nutrients and phytochemicals and evaluate its in vitro biological activity, following a "zero waste" approach. The quince peel dry powder was particularly rich in fiber (20.2 g/100 g), fructose (34 g/100 g), malic acid (7.2 g/100 g), and potassium (692 mg/100 g). Extracts prepared by dynamic hydroethanolic maceration and hot water extraction yielded 4.70 and 4.27 mg/g of phenolic compounds, respectively, with a prevalence of flavan-3-ols. The hydroethanolic extract was the most effective in inhibiting lipid peroxidation and oxidative hemolysis, and also presented better antimicrobial effects against foodborne pathogens, which agreed with the highest flavan-3-ol contents. The extracts were better than control synthetic food additives against some tested fungal and bacterial strains. On the other hand, no ability to inhibit nitric oxide production or toxicity to the tumor and non-tumor cell lines was observed. Furthermore, the solid residues remaining after extraction contained 35-37 g/100 g of fiber. Overall, quince peel can be upcycled into fiber-rich and bioactive ingredients to endow the value chain with natural food fortifiers, preservatives, and health promoters.",
publisher = "Elsevier Ltd.",
journal = "Heliyon",
title = "Valorization of quince peel into functional food ingredients: A path towards "zero waste" and sustainable food systems",
number = "10",
volume = "8",
doi = "10.1016/j.heliyon.2022.e11042",
pages = "e11042"
}

Othman, S., Añibarro-Ortega, M., Dias, M. I., Ćirić, A., Mandim, F., Soković, M., Ferreira, I. C. F. R., Pinela, J.,& Barros, L.. (2022). Valorization of quince peel into functional food ingredients: A path towards "zero waste" and sustainable food systems. in Heliyon
Elsevier Ltd.., 8(10), e11042.
https://doi.org/10.1016/j.heliyon.2022.e11042

Othman S, Añibarro-Ortega M, Dias MI, Ćirić A, Mandim F, Soković M, Ferreira ICFR, Pinela J, Barros L. Valorization of quince peel into functional food ingredients: A path towards "zero waste" and sustainable food systems. in Heliyon. 2022;8(10):e11042.
doi:10.1016/j.heliyon.2022.e11042 .

Othman, Souha, Añibarro-Ortega, Mikel, Dias, Maria Inês, Ćirić, Ana, Mandim, Filipa, Soković, Marina, Ferreira, Isabel C F R, Pinela, José, Barros, Lillian, "Valorization of quince peel into functional food ingredients: A path towards "zero waste" and sustainable food systems" in Heliyon, 8, no. 10 (2022):e11042,
https://doi.org/10.1016/j.heliyon.2022.e11042 . .

TY  - CONF
AU  - Añibarro-Ortega, Mikel
AU  - Pinela, José
AU  - Petrović, Jovana
AU  - Prieto, Miguel Angel
AU  - Soković, Marina
AU  - Ferreira, Isabel
AU  - Simal-Gandara, Jesus
AU  - Barros, Lillian
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6691
AB  - European consumers have a growing interest in new fruits and flavours, which has promoted the production and commercialisation of exotic fruits such as goldenberry (Physalis peruviana L., Fam. Solanaceae). Colombia and South Africa are currently the main producers of this fruit, but it can be cultivated in almost all the highlands of the tropics and in several parts of the subtropics, given its ability to adapt to a wide range of agroecological conditions [1]. Consumer demand for this small orange berry has also been driven by its nutritional value and health-promoting effects [2]. Although there have been different studies about this fruit, the available information is still scarce. Furthermore, the fruit quality attributes can vary depending on the agricultural practices used and the edaphoclimatic conditions of the growing sites. Therefore, this study was carried out to evaluate the nutritional composition and in vitro antioxidant and antimicrobial activity of goldenberry cultivated in the northeast region of Portugal. Goldenberry samples were lyophilised and analysed to determine their proximate composition (moisture, protein, fat, ash, dietary fibre, and carbohydrates), following official methods of food analysis [3]. Their profiles of sugars, organic acids, fatty acids, and tocopherols were characterised by standardised chromatographic methods [3]. The powdered goldenberry sample was also prepared in a hydroethanolic extract used for the evaluation of antioxidant activity (by oxidative haemolysis and lipid peroxidation inhibition assays) and antimicrobial effects against foodborne bacteria and fungi (by serial microdilution methods) [3]. The nutritional analysis revealed high levels of carbohydrates (manly fructose and glucose) and fibre and a lipid fraction consisting mainly of polyunsaturated fatty acids. Citric and ascorbic acids were detected in high amounts, as well as the four tocopherol isoforms. The hydroethanolic berry extract showed capacity for inhibiting haemolytic oxidation and lipid peroxidation, antibacterial effects against Staphylococcus aureus and Bacillus cereus, and antifungal activity against Aspergillus and Penicillium strains. Overall, this work highlighted the nutritional value of goldenberry, which has been noted as a functional fruit with a growing presence in the Portuguese market.
PB  - Basel: MDPI
C3  - The 2nd International Electronic Conference on Foods—“Future Foods and Food Technologies for a Sustainable World; 2021 Oct 15-30; OnlineBiology and Life Sciences Forum
T1  - Nutritional Composition and Biological Activity of Goldenberry (Physalis peruviana L.): An Emerging Fruit Crop in Portugal.
IS  - 3
VL  - 6
DO  - 10.3390/Foods2021-10949
ER  - 

@conference{
author = "Añibarro-Ortega, Mikel and Pinela, José and Petrović, Jovana and Prieto, Miguel Angel and Soković, Marina and Ferreira, Isabel and Simal-Gandara, Jesus and Barros, Lillian",
year = "2021",
abstract = "European consumers have a growing interest in new fruits and flavours, which has promoted the production and commercialisation of exotic fruits such as goldenberry (Physalis peruviana L., Fam. Solanaceae). Colombia and South Africa are currently the main producers of this fruit, but it can be cultivated in almost all the highlands of the tropics and in several parts of the subtropics, given its ability to adapt to a wide range of agroecological conditions [1]. Consumer demand for this small orange berry has also been driven by its nutritional value and health-promoting effects [2]. Although there have been different studies about this fruit, the available information is still scarce. Furthermore, the fruit quality attributes can vary depending on the agricultural practices used and the edaphoclimatic conditions of the growing sites. Therefore, this study was carried out to evaluate the nutritional composition and in vitro antioxidant and antimicrobial activity of goldenberry cultivated in the northeast region of Portugal. Goldenberry samples were lyophilised and analysed to determine their proximate composition (moisture, protein, fat, ash, dietary fibre, and carbohydrates), following official methods of food analysis [3]. Their profiles of sugars, organic acids, fatty acids, and tocopherols were characterised by standardised chromatographic methods [3]. The powdered goldenberry sample was also prepared in a hydroethanolic extract used for the evaluation of antioxidant activity (by oxidative haemolysis and lipid peroxidation inhibition assays) and antimicrobial effects against foodborne bacteria and fungi (by serial microdilution methods) [3]. The nutritional analysis revealed high levels of carbohydrates (manly fructose and glucose) and fibre and a lipid fraction consisting mainly of polyunsaturated fatty acids. Citric and ascorbic acids were detected in high amounts, as well as the four tocopherol isoforms. The hydroethanolic berry extract showed capacity for inhibiting haemolytic oxidation and lipid peroxidation, antibacterial effects against Staphylococcus aureus and Bacillus cereus, and antifungal activity against Aspergillus and Penicillium strains. Overall, this work highlighted the nutritional value of goldenberry, which has been noted as a functional fruit with a growing presence in the Portuguese market.",
publisher = "Basel: MDPI",
journal = "The 2nd International Electronic Conference on Foods—“Future Foods and Food Technologies for a Sustainable World; 2021 Oct 15-30; OnlineBiology and Life Sciences Forum",
title = "Nutritional Composition and Biological Activity of Goldenberry (Physalis peruviana L.): An Emerging Fruit Crop in Portugal.",
number = "3",
volume = "6",
doi = "10.3390/Foods2021-10949"
}

Añibarro-Ortega, M., Pinela, J., Petrović, J., Prieto, M. A., Soković, M., Ferreira, I., Simal-Gandara, J.,& Barros, L.. (2021). Nutritional Composition and Biological Activity of Goldenberry (Physalis peruviana L.): An Emerging Fruit Crop in Portugal.. in The 2nd International Electronic Conference on Foods—“Future Foods and Food Technologies for a Sustainable World; 2021 Oct 15-30; OnlineBiology and Life Sciences Forum
Basel: MDPI., 6(3).
https://doi.org/10.3390/Foods2021-10949

Añibarro-Ortega M, Pinela J, Petrović J, Prieto MA, Soković M, Ferreira I, Simal-Gandara J, Barros L. Nutritional Composition and Biological Activity of Goldenberry (Physalis peruviana L.): An Emerging Fruit Crop in Portugal.. in The 2nd International Electronic Conference on Foods—“Future Foods and Food Technologies for a Sustainable World; 2021 Oct 15-30; OnlineBiology and Life Sciences Forum. 2021;6(3).
doi:10.3390/Foods2021-10949 .

Añibarro-Ortega, Mikel, Pinela, José, Petrović, Jovana, Prieto, Miguel Angel, Soković, Marina, Ferreira, Isabel, Simal-Gandara, Jesus, Barros, Lillian, "Nutritional Composition and Biological Activity of Goldenberry (Physalis peruviana L.): An Emerging Fruit Crop in Portugal." in The 2nd International Electronic Conference on Foods—“Future Foods and Food Technologies for a Sustainable World; 2021 Oct 15-30; OnlineBiology and Life Sciences Forum, 6, no. 3 (2021),
https://doi.org/10.3390/Foods2021-10949 . .

TY  - CONF
AU  - Añibarro-Ortega, Mikel
AU  - Pinela, José
AU  - Ćirić, Ana
AU  - Rocha, Filomena
AU  - Barata, Ana Maria
AU  - Soković, Marina
AU  - Carvalho, Ana Maria
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4374
AB  - Table tomato (Solanum lycopersicum L.) is highly consumed worldwide and its annual production generates 
a considerable amount of plant by-products [1]. Although tomatoes are well-known for their composition 
in nutrients and bioactive compounds [2,3], little information is available in literature regarding tomato 
leaves, probably because these by-products have no commercial value. Therefore, considering the enormous
production of this biomass and the current trends of circularity and sustainable development [3], this study 
was carried out to evaluate the polyphenolic composition and bioactive properties of tomato leaves resulting 
from the regeneration of table tomato landraces conserved ex-situ in the Portuguese Genebank (BPGV), in 
Braga, Portugal. The tomato plant material resulting from pruning performed after the flowering season was
lyophilized and ground to a fine powder, used to prepare hydroethanolic extracts [4]. These extracts were 
characterized for their composition in polyphenols (by HPLC-DAD-ESI/MSn
) and used to evaluate the 
antioxidant (by DPPH•
scavenging capacity and reduction power), antihemolytic (using sheep erythrocytes 
and AAPH as a free radical generator), and antimicrobial (tested against food-borne bacterial and fungal 
strains by the serial microdilution and p-iodonitrotetrazolium violet colorimetric methods) activities [4].
The chromatographic analysis allowed identifying phenolic acids and flavonoids, with prevalence of 
quercetin-3-O-rutinoside (rutin). The leaf extracts showed antioxidant activity, with EC50 values lower than 
those previously reported for the respective tomato fruits, thus translating a higher activity [2]. The extracts 
were also effective in some extent in protecting the erythrocytes from the oxidative hemolysis caused the 
thermal decomposition of the free-radical initiator AAPH. Despite their low activity against the tested 
microfungi, some extracts had ability to inhibit and kill some bacteria (including Salmonella typhimurium, 
Listeria monocytogenes, Bacillus cereus, and Enterobacter cloacae) more effectively than the antibiotic 
ampicillin. It was concluded that table tomato crop by-products can be used to produce rutin-rich extracts 
with antioxidant and antibacterial activities for possible use in the agri-food sector as natural preservatives.
PB  - Bragança, Portugal: Instituto Politécnico de Bragança (IPB)
C3  - 1st Natural products application: Health, Cosmetic and Food: book of abstracts
T1  - Table tomato leaves are a sustainable source of rutin and display antioxidant, antihemolytic and antimicrobial activity
SP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4374
ER  - 

@conference{
author = "Añibarro-Ortega, Mikel and Pinela, José and Ćirić, Ana and Rocha, Filomena and Barata, Ana Maria and Soković, Marina and Carvalho, Ana Maria and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2021",
abstract = "Table tomato (Solanum lycopersicum L.) is highly consumed worldwide and its annual production generates 
a considerable amount of plant by-products [1]. Although tomatoes are well-known for their composition 
in nutrients and bioactive compounds [2,3], little information is available in literature regarding tomato 
leaves, probably because these by-products have no commercial value. Therefore, considering the enormous
production of this biomass and the current trends of circularity and sustainable development [3], this study 
was carried out to evaluate the polyphenolic composition and bioactive properties of tomato leaves resulting 
from the regeneration of table tomato landraces conserved ex-situ in the Portuguese Genebank (BPGV), in 
Braga, Portugal. The tomato plant material resulting from pruning performed after the flowering season was
lyophilized and ground to a fine powder, used to prepare hydroethanolic extracts [4]. These extracts were 
characterized for their composition in polyphenols (by HPLC-DAD-ESI/MSn
) and used to evaluate the 
antioxidant (by DPPH•
scavenging capacity and reduction power), antihemolytic (using sheep erythrocytes 
and AAPH as a free radical generator), and antimicrobial (tested against food-borne bacterial and fungal 
strains by the serial microdilution and p-iodonitrotetrazolium violet colorimetric methods) activities [4].
The chromatographic analysis allowed identifying phenolic acids and flavonoids, with prevalence of 
quercetin-3-O-rutinoside (rutin). The leaf extracts showed antioxidant activity, with EC50 values lower than 
those previously reported for the respective tomato fruits, thus translating a higher activity [2]. The extracts 
were also effective in some extent in protecting the erythrocytes from the oxidative hemolysis caused the 
thermal decomposition of the free-radical initiator AAPH. Despite their low activity against the tested 
microfungi, some extracts had ability to inhibit and kill some bacteria (including Salmonella typhimurium, 
Listeria monocytogenes, Bacillus cereus, and Enterobacter cloacae) more effectively than the antibiotic 
ampicillin. It was concluded that table tomato crop by-products can be used to produce rutin-rich extracts 
with antioxidant and antibacterial activities for possible use in the agri-food sector as natural preservatives.",
publisher = "Bragança, Portugal: Instituto Politécnico de Bragança (IPB)",
journal = "1st Natural products application: Health, Cosmetic and Food: book of abstracts",
title = "Table tomato leaves are a sustainable source of rutin and display antioxidant, antihemolytic and antimicrobial activity",
pages = "73",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4374"
}

Añibarro-Ortega, M., Pinela, J., Ćirić, A., Rocha, F., Barata, A. M., Soković, M., Carvalho, A. M., Ferreira, I. C. F. R.,& Barros, L.. (2021). Table tomato leaves are a sustainable source of rutin and display antioxidant, antihemolytic and antimicrobial activity. in 1st Natural products application: Health, Cosmetic and Food: book of abstracts
Bragança, Portugal: Instituto Politécnico de Bragança (IPB)., 73.
https://hdl.handle.net/21.15107/rcub_ibiss_4374

Añibarro-Ortega M, Pinela J, Ćirić A, Rocha F, Barata AM, Soković M, Carvalho AM, Ferreira ICFR, Barros L. Table tomato leaves are a sustainable source of rutin and display antioxidant, antihemolytic and antimicrobial activity. in 1st Natural products application: Health, Cosmetic and Food: book of abstracts. 2021;:73.
https://hdl.handle.net/21.15107/rcub_ibiss_4374 .

Añibarro-Ortega, Mikel, Pinela, José, Ćirić, Ana, Rocha, Filomena, Barata, Ana Maria, Soković, Marina, Carvalho, Ana Maria, Ferreira, Isabel C. F. R., Barros, Lillian, "Table tomato leaves are a sustainable source of rutin and display antioxidant, antihemolytic and antimicrobial activity" in 1st Natural products application: Health, Cosmetic and Food: book of abstracts (2021):73,
https://hdl.handle.net/21.15107/rcub_ibiss_4374 .

TY  - JOUR
AU  - Añibarro-Ortega, Mikel
AU  - Pinela, José
AU  - Ćirić, Ana
AU  - Lopes, Elsa
AU  - Molina, Adriana K.
AU  - Calhelha, Ricardo C.
AU  - Soković, Marina
AU  - Ferreira, Olga
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2021
UR  - https://www.mdpi.com/2079-7737/10/10/951
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4488
AB  - Aloesin is an aromatic chromone with increasing applications in the cosmetic and health food industries. To optimize its extraction from the Aloe vera leaf rind, the independent variables time (10–210 min), temperature (25–95 °C) and organic solvent composition (0–100%, w/w) were combined in a central composite design coupled with response surface methodology. The solvents consisted of binary mixtures of water with ethanol, propylene glycol, or glycerol. The aloesin levels quantified in each extract were used as response for optimization. The theoretical models were fitted to the experimental data, statistically validated, and used to obtain the optimal extraction conditions. Then, a dose–response analysis of the solid/liquid ratio (S/L) was performed under the optimal conditions determined for each alcohol–water system and revealed that a linear improvement in extraction efficiency can be achieved by increasing the S/L ratio by up to 40 g/L. This analysis also allowed to experimentally validate the predictive models. Furthermore, the aloesin-rich extracts revealed antioxidant activity through thiobarbituric acid reactive substances (TBARS) formation inhibition, antimicrobial effects against bacterial and fungal strains, and no toxicity for PLP2 cells. Overall, this study provided optimal extraction conditions for the recovery of aloesin from Aloe vera rind through an eco-friendly extraction process and highlighted its bioactive potential.
PB  - Basel: MDPI
T2  - Biology
T1  - Extraction of Aloesin from Aloe vera Rind Using Alternative Green Solvents: Process Optimization and Biological Activity Assessment
IS  - 10
VL  - 10
DO  - 10.3390/biology10100951
SP  - 951
ER  - 

@article{
author = "Añibarro-Ortega, Mikel and Pinela, José and Ćirić, Ana and Lopes, Elsa and Molina, Adriana K. and Calhelha, Ricardo C. and Soković, Marina and Ferreira, Olga and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2021",
abstract = "Aloesin is an aromatic chromone with increasing applications in the cosmetic and health food industries. To optimize its extraction from the Aloe vera leaf rind, the independent variables time (10–210 min), temperature (25–95 °C) and organic solvent composition (0–100%, w/w) were combined in a central composite design coupled with response surface methodology. The solvents consisted of binary mixtures of water with ethanol, propylene glycol, or glycerol. The aloesin levels quantified in each extract were used as response for optimization. The theoretical models were fitted to the experimental data, statistically validated, and used to obtain the optimal extraction conditions. Then, a dose–response analysis of the solid/liquid ratio (S/L) was performed under the optimal conditions determined for each alcohol–water system and revealed that a linear improvement in extraction efficiency can be achieved by increasing the S/L ratio by up to 40 g/L. This analysis also allowed to experimentally validate the predictive models. Furthermore, the aloesin-rich extracts revealed antioxidant activity through thiobarbituric acid reactive substances (TBARS) formation inhibition, antimicrobial effects against bacterial and fungal strains, and no toxicity for PLP2 cells. Overall, this study provided optimal extraction conditions for the recovery of aloesin from Aloe vera rind through an eco-friendly extraction process and highlighted its bioactive potential.",
publisher = "Basel: MDPI",
journal = "Biology",
title = "Extraction of Aloesin from Aloe vera Rind Using Alternative Green Solvents: Process Optimization and Biological Activity Assessment",
number = "10",
volume = "10",
doi = "10.3390/biology10100951",
pages = "951"
}

Añibarro-Ortega, M., Pinela, J., Ćirić, A., Lopes, E., Molina, A. K., Calhelha, R. C., Soković, M., Ferreira, O., Ferreira, I. C. F. R.,& Barros, L.. (2021). Extraction of Aloesin from Aloe vera Rind Using Alternative Green Solvents: Process Optimization and Biological Activity Assessment. in Biology
Basel: MDPI., 10(10), 951.
https://doi.org/10.3390/biology10100951

Añibarro-Ortega M, Pinela J, Ćirić A, Lopes E, Molina AK, Calhelha RC, Soković M, Ferreira O, Ferreira ICFR, Barros L. Extraction of Aloesin from Aloe vera Rind Using Alternative Green Solvents: Process Optimization and Biological Activity Assessment. in Biology. 2021;10(10):951.
doi:10.3390/biology10100951 .

Añibarro-Ortega, Mikel, Pinela, José, Ćirić, Ana, Lopes, Elsa, Molina, Adriana K., Calhelha, Ricardo C., Soković, Marina, Ferreira, Olga, Ferreira, Isabel C. F. R., Barros, Lillian, "Extraction of Aloesin from Aloe vera Rind Using Alternative Green Solvents: Process Optimization and Biological Activity Assessment" in Biology, 10, no. 10 (2021):951,
https://doi.org/10.3390/biology10100951 . .

TY  - JOUR
AU  - Añibarro-Ortega, Mikel
AU  - Pinela, José
AU  - Ćirić, Ana
AU  - Martins, Valter
AU  - Rocha, Filomena
AU  - Soković, Marina
AU  - Barata, Ana Maria
AU  - Carvalho, Ana Maria
AU  - Barros, Lillian
AU  - Ferreira, Isabel C.F.R.
PY  - 2020
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0960308520305174
UR  - https://radar.ibiss.bg.ac.rs/123456789/3906
AB  - Little attention has been paid to the phenolic composition and bioactive properties of primary tomato plant by-products (Solanum lycopersicum L.) as compared to fruits. In this study, axillary green shoots resulting from pruning and aerial biomass at the end of the cultivation cycle were characterized for their composition in chlorophylls and phenolic compounds, as well as for antioxidant and antimicrobial activities. The HPLC-DAD-ESI/MSn analysis allowed identifying phenolic acids and flavonoids, with prevalence of quercetin-3-O-rutinoside (rutin). The extracts had in vitro antioxidant and antihemolytic activity, especially those produced from pruning materials. Despite their low activity against the tested microfungi, some extracts had ability to inhibit and kill some bacteria more effectively than the antibiotic ampicillin. It was interesting to conclude that table tomato crop remains, currently with no commercial value, can be used to produce extracts with antioxidant and antibacterial activities for possible use in the agri-food sector as natural preservatives.
PB  - Elsevier BV
T2  - Food and Bioproducts Processing
T1  - Valorisation of table tomato crop by-products: Phenolic profiles and in vitro antioxidant and antimicrobial activities
VL  - 124
DO  - 10.1016/j.fbp.2020.09.006
SP  - 307
EP  - 319
ER  - 

@article{
author = "Añibarro-Ortega, Mikel and Pinela, José and Ćirić, Ana and Martins, Valter and Rocha, Filomena and Soković, Marina and Barata, Ana Maria and Carvalho, Ana Maria and Barros, Lillian and Ferreira, Isabel C.F.R.",
year = "2020",
abstract = "Little attention has been paid to the phenolic composition and bioactive properties of primary tomato plant by-products (Solanum lycopersicum L.) as compared to fruits. In this study, axillary green shoots resulting from pruning and aerial biomass at the end of the cultivation cycle were characterized for their composition in chlorophylls and phenolic compounds, as well as for antioxidant and antimicrobial activities. The HPLC-DAD-ESI/MSn analysis allowed identifying phenolic acids and flavonoids, with prevalence of quercetin-3-O-rutinoside (rutin). The extracts had in vitro antioxidant and antihemolytic activity, especially those produced from pruning materials. Despite their low activity against the tested microfungi, some extracts had ability to inhibit and kill some bacteria more effectively than the antibiotic ampicillin. It was interesting to conclude that table tomato crop remains, currently with no commercial value, can be used to produce extracts with antioxidant and antibacterial activities for possible use in the agri-food sector as natural preservatives.",
publisher = "Elsevier BV",
journal = "Food and Bioproducts Processing",
title = "Valorisation of table tomato crop by-products: Phenolic profiles and in vitro antioxidant and antimicrobial activities",
volume = "124",
doi = "10.1016/j.fbp.2020.09.006",
pages = "307-319"
}

Añibarro-Ortega, M., Pinela, J., Ćirić, A., Martins, V., Rocha, F., Soković, M., Barata, A. M., Carvalho, A. M., Barros, L.,& Ferreira, I. C.F.R.. (2020). Valorisation of table tomato crop by-products: Phenolic profiles and in vitro antioxidant and antimicrobial activities. in Food and Bioproducts Processing
Elsevier BV., 124, 307-319.
https://doi.org/10.1016/j.fbp.2020.09.006

Añibarro-Ortega M, Pinela J, Ćirić A, Martins V, Rocha F, Soković M, Barata AM, Carvalho AM, Barros L, Ferreira IC. Valorisation of table tomato crop by-products: Phenolic profiles and in vitro antioxidant and antimicrobial activities. in Food and Bioproducts Processing. 2020;124:307-319.
doi:10.1016/j.fbp.2020.09.006 .

Añibarro-Ortega, Mikel, Pinela, José, Ćirić, Ana, Martins, Valter, Rocha, Filomena, Soković, Marina, Barata, Ana Maria, Carvalho, Ana Maria, Barros, Lillian, Ferreira, Isabel C.F.R., "Valorisation of table tomato crop by-products: Phenolic profiles and in vitro antioxidant and antimicrobial activities" in Food and Bioproducts Processing, 124 (2020):307-319,
https://doi.org/10.1016/j.fbp.2020.09.006 . .

TY  - JOUR
AU  - Añibarro-Ortega, Mikel
AU  - Pinela, José
AU  - Barros, Lillian
AU  - Ćirić, Ana
AU  - Silva, Soraia P.
AU  - Coelho, Elisabete
AU  - Mocan, Andrei
AU  - Calhelha, Ricardo C.
AU  - Soković, Marina
AU  - Coimbra, Manuel A.
AU  - Ferreira, Isabel C. F. R.
PY  - 2019
UR  - https://www.mdpi.com/2076-3921/8/10/444
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3491
AB  - This work aimed to characterize compositional and bioactive features of Aloe vera leaf (fillet, mucilage, and rind) and flower. The edible fillet was analysed for its nutritional value, and all samples were studied for phenolic composition and antioxidant, anti-inflammatory, antimicrobial, tyrosinase inhibition, and cytotoxic activities. Dietary fibre (mainly mannan) and available carbohydrates (mainly free glucose and fructose) were abundant macronutrients in fillet, which also contained high amounts of malic acid (5.75 g/100 g dw) and α-tocopherol (4.8 mg/100 g dw). The leaf samples presented similar phenolic profiles, with predominance of chromones and anthrones, and the highest contents were found in mucilage (131 mg/g) and rind (105 mg/g) extracts, which also revealed interesting antioxidant properties. On the other hand, the flower extract was rich in apigenin glycoside derivatives (4.48 mg/g), effective against Pseudomonas aeruginosa (MIC = 0.025 mg/mL and MBC = 0.05 mg/mL) and capable of inhibiting the tyrosinase activity (IC50 = 4.85 mg/mL). The fillet, rind, and flower extracts also showed a powerful antifungal activity against Aspergillus flavus, A. niger, Penicillium funiculosum, and Candida albicans, higher than that of ketoconazole. Thus, the studied Aloe vera samples displayed high potential to be exploited by the food or cosmetic industries, among others.
T2  - Antioxidants (Basel, Switzerland)
T1  - Compositional Features and Bioactive Properties of Aloe vera Leaf (Fillet, Mucilage, and Rind) and Flower
IS  - 10
VL  - 8
DO  - 10.3390/antiox8100444
SP  - 444
ER  - 

@article{
author = "Añibarro-Ortega, Mikel and Pinela, José and Barros, Lillian and Ćirić, Ana and Silva, Soraia P. and Coelho, Elisabete and Mocan, Andrei and Calhelha, Ricardo C. and Soković, Marina and Coimbra, Manuel A. and Ferreira, Isabel C. F. R.",
year = "2019",
abstract = "This work aimed to characterize compositional and bioactive features of Aloe vera leaf (fillet, mucilage, and rind) and flower. The edible fillet was analysed for its nutritional value, and all samples were studied for phenolic composition and antioxidant, anti-inflammatory, antimicrobial, tyrosinase inhibition, and cytotoxic activities. Dietary fibre (mainly mannan) and available carbohydrates (mainly free glucose and fructose) were abundant macronutrients in fillet, which also contained high amounts of malic acid (5.75 g/100 g dw) and α-tocopherol (4.8 mg/100 g dw). The leaf samples presented similar phenolic profiles, with predominance of chromones and anthrones, and the highest contents were found in mucilage (131 mg/g) and rind (105 mg/g) extracts, which also revealed interesting antioxidant properties. On the other hand, the flower extract was rich in apigenin glycoside derivatives (4.48 mg/g), effective against Pseudomonas aeruginosa (MIC = 0.025 mg/mL and MBC = 0.05 mg/mL) and capable of inhibiting the tyrosinase activity (IC50 = 4.85 mg/mL). The fillet, rind, and flower extracts also showed a powerful antifungal activity against Aspergillus flavus, A. niger, Penicillium funiculosum, and Candida albicans, higher than that of ketoconazole. Thus, the studied Aloe vera samples displayed high potential to be exploited by the food or cosmetic industries, among others.",
journal = "Antioxidants (Basel, Switzerland)",
title = "Compositional Features and Bioactive Properties of Aloe vera Leaf (Fillet, Mucilage, and Rind) and Flower",
number = "10",
volume = "8",
doi = "10.3390/antiox8100444",
pages = "444"
}

Añibarro-Ortega, M., Pinela, J., Barros, L., Ćirić, A., Silva, S. P., Coelho, E., Mocan, A., Calhelha, R. C., Soković, M., Coimbra, M. A.,& Ferreira, I. C. F. R.. (2019). Compositional Features and Bioactive Properties of Aloe vera Leaf (Fillet, Mucilage, and Rind) and Flower. in Antioxidants (Basel, Switzerland), 8(10), 444.
https://doi.org/10.3390/antiox8100444

Añibarro-Ortega M, Pinela J, Barros L, Ćirić A, Silva SP, Coelho E, Mocan A, Calhelha RC, Soković M, Coimbra MA, Ferreira ICFR. Compositional Features and Bioactive Properties of Aloe vera Leaf (Fillet, Mucilage, and Rind) and Flower. in Antioxidants (Basel, Switzerland). 2019;8(10):444.
doi:10.3390/antiox8100444 .

Añibarro-Ortega, Mikel, Pinela, José, Barros, Lillian, Ćirić, Ana, Silva, Soraia P., Coelho, Elisabete, Mocan, Andrei, Calhelha, Ricardo C., Soković, Marina, Coimbra, Manuel A., Ferreira, Isabel C. F. R., "Compositional Features and Bioactive Properties of Aloe vera Leaf (Fillet, Mucilage, and Rind) and Flower" in Antioxidants (Basel, Switzerland), 8, no. 10 (2019):444,
https://doi.org/10.3390/antiox8100444 . .