TY  - JOUR
AU  - Alimpić-Aradski, Ana
AU  - Olađe Pavlović, Mariana
AU  - Janošević, Dušica
AU  - Todorović, Slađana
AU  - Gašić, Uroš
AU  - Mišić, Danijela
AU  - Pljevljakušić, Dejan
AU  - Šavikin, Katarina
AU  - Marin, Petar D.
AU  - Giweli, Abdulhmid
AU  - Duletić-Laušević, Sonja
PY  - 2023
UR  - http://www3.interscience.wiley.com/cgi-bin/jhome/5152
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6445
AB  - Introduction: The endemic species Nepeta cyrenaica Quézel & Zaffran, native to northeastern Libya, is valued as an important honey-bearing plant.

Objectives: This study was aimed to examine the micromorphology, phytochemistry, and bioactivity of in vitro-propagated N. cyrenaica for the first time.

Materials and methods: The leaf indumentum was examined using light and scanning electron microscopy and further characterised for histochemistry. The chemical composition of essential oil (EO) was performed using GC-MS analysis, while dichloromethane (DCM), methanol (ME), ethanol (ET), and aqueous (AQ) extracts were analysed using qualitative and quantitative LC/MS analyses. The antioxidant activities of EO and extracts were assessed using three parallel assays, while enzyme-inhibiting effects were evaluated against four enzymes.

Results: The leaves bear various types of glandular trichomes, with lipophilic secretion predominating. The main EO component of EO was 1,8-cineole. A considerable number of phenolics and iridoids were tentatively identified in the ME extract. Quantitative LC/MS analysis confirmed that ferulic acid, rosmarinic acid, and epigallocatechin gallate were present in the highest amount in the extracts, in which three iridoids were also quantified. Although the ME extract contained the highest amount of polyphenolics and iridoids, the DCM extract showed the best overall biological potential. Additionally, EO exerted the strongest acetylcholinesterase and tyrosinase inhibition.

Conclusion: This study demonstrated that the endemic N. cyrenaica can be efficiently grown under in vitro conditions, where it develops various glandular trichomes that are thought to secrete and/or accumulate bioactive compounds with valuable medicinal potential.
PB  - Hoboken: John Wiley and Sons
T2  - Phytochemical Analysis
T1  - Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae)
IS  - 6
VL  - 34
DO  - 10.1002/pca.3257
SP  - 661
EP  - 679
ER  - 

@article{
author = "Alimpić-Aradski, Ana and Olađe Pavlović, Mariana and Janošević, Dušica and Todorović, Slađana and Gašić, Uroš and Mišić, Danijela and Pljevljakušić, Dejan and Šavikin, Katarina and Marin, Petar D. and Giweli, Abdulhmid and Duletić-Laušević, Sonja",
year = "2023",
abstract = "Introduction: The endemic species Nepeta cyrenaica Quézel & Zaffran, native to northeastern Libya, is valued as an important honey-bearing plant.

Objectives: This study was aimed to examine the micromorphology, phytochemistry, and bioactivity of in vitro-propagated N. cyrenaica for the first time.

Materials and methods: The leaf indumentum was examined using light and scanning electron microscopy and further characterised for histochemistry. The chemical composition of essential oil (EO) was performed using GC-MS analysis, while dichloromethane (DCM), methanol (ME), ethanol (ET), and aqueous (AQ) extracts were analysed using qualitative and quantitative LC/MS analyses. The antioxidant activities of EO and extracts were assessed using three parallel assays, while enzyme-inhibiting effects were evaluated against four enzymes.

Results: The leaves bear various types of glandular trichomes, with lipophilic secretion predominating. The main EO component of EO was 1,8-cineole. A considerable number of phenolics and iridoids were tentatively identified in the ME extract. Quantitative LC/MS analysis confirmed that ferulic acid, rosmarinic acid, and epigallocatechin gallate were present in the highest amount in the extracts, in which three iridoids were also quantified. Although the ME extract contained the highest amount of polyphenolics and iridoids, the DCM extract showed the best overall biological potential. Additionally, EO exerted the strongest acetylcholinesterase and tyrosinase inhibition.

Conclusion: This study demonstrated that the endemic N. cyrenaica can be efficiently grown under in vitro conditions, where it develops various glandular trichomes that are thought to secrete and/or accumulate bioactive compounds with valuable medicinal potential.",
publisher = "Hoboken: John Wiley and Sons",
journal = "Phytochemical Analysis",
title = "Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae)",
number = "6",
volume = "34",
doi = "10.1002/pca.3257",
pages = "661-679"
}

Alimpić-Aradski, A., Olađe Pavlović, M., Janošević, D., Todorović, S., Gašić, U., Mišić, D., Pljevljakušić, D., Šavikin, K., Marin, P. D., Giweli, A.,& Duletić-Laušević, S.. (2023). Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae). in Phytochemical Analysis
Hoboken: John Wiley and Sons., 34(6), 661-679.
https://doi.org/10.1002/pca.3257

Alimpić-Aradski A, Olađe Pavlović M, Janošević D, Todorović S, Gašić U, Mišić D, Pljevljakušić D, Šavikin K, Marin PD, Giweli A, Duletić-Laušević S. Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae). in Phytochemical Analysis. 2023;34(6):661-679.
doi:10.1002/pca.3257 .

Alimpić-Aradski, Ana, Olađe Pavlović, Mariana, Janošević, Dušica, Todorović, Slađana, Gašić, Uroš, Mišić, Danijela, Pljevljakušić, Dejan, Šavikin, Katarina, Marin, Petar D., Giweli, Abdulhmid, Duletić-Laušević, Sonja, "Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae)" in Phytochemical Analysis, 34, no. 6 (2023):661-679,
https://doi.org/10.1002/pca.3257 . .

TY  - JOUR
AU  - Đorđevski, Nikoleta
AU  - Stojković, Dejan
AU  - Živković, Jelena
AU  - Pljevljakušić, Dejan
AU  - Ristanović, Elizabeta
AU  - Nikolić, Biljana
AU  - Ćirić, Ana
PY  - 2022
UR  - https://onlinelibrary.wiley.com/doi/10.1002/fsn3.2777
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4864
AB  - This study was designed to explore functional food properties of edible seed oil obtained from Tamjanika seeds—autochthonous grape variety of Balkan Peninsula. In order to accomplish our goals, seed oil was isolated by Soxhlet apparatus and chemically characterized regarding fatty acids, carotenoids, tocopherols, and tocotrienols. Antimicrobial activity of the isolated oil was tested by microdilution method. For that purposes, six bacterial species were used, belonging to human infectious agents and food contaminants. Furthermore, the activity of the oil was investigated against clinical isolates of dermatomycetes. Our study has shown that oil of Vitis vinifera L. Tamjanika variety was an abundant source of polyunsaturated fatty acids (81.43%) with predominant linoleic acid. HPLC analysis revealed the presence of carotenoid lutein (0.15 mg/100 g). The seed oil was rich in tocotrienols (85.04 mg/100 g) predominating over tocopherols (8.37 mg/100 g). The oil possessed microbicidal activity against all the tested microbes. Bacteria were more sensitive to the effect of the oil (minimum inhibitory concentration [MIC] 7.7–15.4) when compared with oil effect on tested dermatomycetes (MIC 20–40). Our investigation has shown for the first time that grape oil could be active against wide spectrum of bacteria and clinically isolated dermatomycetes. The significance of this study lies in the fact that it pointed out the functional food properties of grape seed oil that was fully chemically characterized.
PB  - John Wiley and Sons Inc
T2  - Food Science & Nutrition
T1  - Tamjanika, a Balkan native variety of Vitis vinifera L.: Chemical characterization, antibacterial, and anti‐dermatomycosis potential of seed oil
IS  - 4
VL  - 10
DO  - 10.1002/fsn3.2777
SP  - 1312
EP  - 1319
ER  - 

@article{
author = "Đorđevski, Nikoleta and Stojković, Dejan and Živković, Jelena and Pljevljakušić, Dejan and Ristanović, Elizabeta and Nikolić, Biljana and Ćirić, Ana",
year = "2022",
abstract = "This study was designed to explore functional food properties of edible seed oil obtained from Tamjanika seeds—autochthonous grape variety of Balkan Peninsula. In order to accomplish our goals, seed oil was isolated by Soxhlet apparatus and chemically characterized regarding fatty acids, carotenoids, tocopherols, and tocotrienols. Antimicrobial activity of the isolated oil was tested by microdilution method. For that purposes, six bacterial species were used, belonging to human infectious agents and food contaminants. Furthermore, the activity of the oil was investigated against clinical isolates of dermatomycetes. Our study has shown that oil of Vitis vinifera L. Tamjanika variety was an abundant source of polyunsaturated fatty acids (81.43%) with predominant linoleic acid. HPLC analysis revealed the presence of carotenoid lutein (0.15 mg/100 g). The seed oil was rich in tocotrienols (85.04 mg/100 g) predominating over tocopherols (8.37 mg/100 g). The oil possessed microbicidal activity against all the tested microbes. Bacteria were more sensitive to the effect of the oil (minimum inhibitory concentration [MIC] 7.7–15.4) when compared with oil effect on tested dermatomycetes (MIC 20–40). Our investigation has shown for the first time that grape oil could be active against wide spectrum of bacteria and clinically isolated dermatomycetes. The significance of this study lies in the fact that it pointed out the functional food properties of grape seed oil that was fully chemically characterized.",
publisher = "John Wiley and Sons Inc",
journal = "Food Science & Nutrition",
title = "Tamjanika, a Balkan native variety of Vitis vinifera L.: Chemical characterization, antibacterial, and anti‐dermatomycosis potential of seed oil",
number = "4",
volume = "10",
doi = "10.1002/fsn3.2777",
pages = "1312-1319"
}

Đorđevski, N., Stojković, D., Živković, J., Pljevljakušić, D., Ristanović, E., Nikolić, B.,& Ćirić, A.. (2022). Tamjanika, a Balkan native variety of Vitis vinifera L.: Chemical characterization, antibacterial, and anti‐dermatomycosis potential of seed oil. in Food Science & Nutrition
John Wiley and Sons Inc., 10(4), 1312-1319.
https://doi.org/10.1002/fsn3.2777

Đorđevski N, Stojković D, Živković J, Pljevljakušić D, Ristanović E, Nikolić B, Ćirić A. Tamjanika, a Balkan native variety of Vitis vinifera L.: Chemical characterization, antibacterial, and anti‐dermatomycosis potential of seed oil. in Food Science & Nutrition. 2022;10(4):1312-1319.
doi:10.1002/fsn3.2777 .

Đorđevski, Nikoleta, Stojković, Dejan, Živković, Jelena, Pljevljakušić, Dejan, Ristanović, Elizabeta, Nikolić, Biljana, Ćirić, Ana, "Tamjanika, a Balkan native variety of Vitis vinifera L.: Chemical characterization, antibacterial, and anti‐dermatomycosis potential of seed oil" in Food Science & Nutrition, 10, no. 4 (2022):1312-1319,
https://doi.org/10.1002/fsn3.2777 . .

TY  - JOUR
AU  - Šavikin, Katarina P.
AU  - Zdunić, Gordana M.
AU  - Krstić Milošević, Dijana
AU  - Šircelj, Helena J.
AU  - Stešević, Danijela D.
AU  - Pljevljakušić, Dejan S.
PY  - 2017
UR  - http://doi.wiley.com/10.1002/cbdv.201700329
UR  - http://www.ncbi.nlm.nih.gov/pubmed/28845915
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2950
AB  - Due to its nutritive and medicinal properties, berries of some Sorbus species are used for the preparation of jams and jelly as well as in traditional medicine. On the other hand, their chemical composition is not much studied especially of those grown in Balkan Peninsula. We have analyzed individual phenolics, tocopherols, carotenoids and chlorophylls using HPLC in berries from Sorbus aucuparia and Sorbus aria collected in different localities in Serbia and Montenegro together with the amounts of total phenolics and proanthocyanidins as well as their radical scavenging activity against DPPH radical. Berries of S. aucuparia were richer source of polyphenolics in comparision with S. aria and, regardless the species and locality, caffeoylquinic acids such as neochlorogenic and chlorogenic acid were the most abundant compounds. Among analyzed tocopherols the most abundant in all samples was α-tocopherol (0.48 - 19.85 μg/g dw) as it was β-carotene among carotenoids (mean concentration of 0.98 μg/g dw in S. aucuparia and 0.40 μg/g dw in S. aria, respectively). Correlation between total phenolics and DPPH radical scavenging activity was noticed. Our study represents comprehensive report on chemical composition of S. aucuparia and S. aria which could contribute to a better understanding of their quality.
T2  - Chemistry & Biodiversity
T1  - Sorbus aucuparia and Sorbus aria as a Source of Antioxidant Phenolics, Tocopherols, and Pigments.
IS  - 12
VL  - 14
DO  - 10.1002/cbdv.201700329
SP  - e1700329
ER  - 

@article{
author = "Šavikin, Katarina P. and Zdunić, Gordana M. and Krstić Milošević, Dijana and Šircelj, Helena J. and Stešević, Danijela D. and Pljevljakušić, Dejan S.",
year = "2017",
abstract = "Due to its nutritive and medicinal properties, berries of some Sorbus species are used for the preparation of jams and jelly as well as in traditional medicine. On the other hand, their chemical composition is not much studied especially of those grown in Balkan Peninsula. We have analyzed individual phenolics, tocopherols, carotenoids and chlorophylls using HPLC in berries from Sorbus aucuparia and Sorbus aria collected in different localities in Serbia and Montenegro together with the amounts of total phenolics and proanthocyanidins as well as their radical scavenging activity against DPPH radical. Berries of S. aucuparia were richer source of polyphenolics in comparision with S. aria and, regardless the species and locality, caffeoylquinic acids such as neochlorogenic and chlorogenic acid were the most abundant compounds. Among analyzed tocopherols the most abundant in all samples was α-tocopherol (0.48 - 19.85 μg/g dw) as it was β-carotene among carotenoids (mean concentration of 0.98 μg/g dw in S. aucuparia and 0.40 μg/g dw in S. aria, respectively). Correlation between total phenolics and DPPH radical scavenging activity was noticed. Our study represents comprehensive report on chemical composition of S. aucuparia and S. aria which could contribute to a better understanding of their quality.",
journal = "Chemistry & Biodiversity",
title = "Sorbus aucuparia and Sorbus aria as a Source of Antioxidant Phenolics, Tocopherols, and Pigments.",
number = "12",
volume = "14",
doi = "10.1002/cbdv.201700329",
pages = "e1700329"
}

Šavikin, K. P., Zdunić, G. M., Krstić Milošević, D., Šircelj, H. J., Stešević, D. D.,& Pljevljakušić, D. S.. (2017). Sorbus aucuparia and Sorbus aria as a Source of Antioxidant Phenolics, Tocopherols, and Pigments.. in Chemistry & Biodiversity, 14(12), e1700329.
https://doi.org/10.1002/cbdv.201700329

Šavikin KP, Zdunić GM, Krstić Milošević D, Šircelj HJ, Stešević DD, Pljevljakušić DS. Sorbus aucuparia and Sorbus aria as a Source of Antioxidant Phenolics, Tocopherols, and Pigments.. in Chemistry & Biodiversity. 2017;14(12):e1700329.
doi:10.1002/cbdv.201700329 .

Šavikin, Katarina P., Zdunić, Gordana M., Krstić Milošević, Dijana, Šircelj, Helena J., Stešević, Danijela D., Pljevljakušić, Dejan S., "Sorbus aucuparia and Sorbus aria as a Source of Antioxidant Phenolics, Tocopherols, and Pigments." in Chemistry & Biodiversity, 14, no. 12 (2017):e1700329,
https://doi.org/10.1002/cbdv.201700329 . .

TY  - CONF
AU  - Šavikin, Katarina P.
AU  - Krstić Milošević, Dijana
AU  - Menković, Nebojša R.
AU  - Beara, Ivana N.
AU  - Mrkonjić, Zorica O.
AU  - Pljevljakušić, Dejan S.
PY  - 2017
UR  - http://www.naturalproduct.us/
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2756
AB  - The present study was designed to define the phenolic content, antioxidant and anti-inflammatory activity of Crateagus orientalis Pall. Ex M. Bieb., traditionally used by local people in southern parts of F.Y.R. Macedonia. The presence and content of 7 phenolics in ethanolic extracts of leaves and berries were studied using HPLC-DAD, where the most dominant compounds were hyperoside, isoquercitrin and chlorogenic acid. The leaf extract was more effective as a DPPH radical scavenger (IC50 = 29.7 μg/g) than the berry extract, as well as in the relative reducing power on Fe3+. Anti-inflammatory potential was studied by means of cyclooxygenase-1 (COX-1) and 12-lipoxygenase (12-LOX) inhibitory activity; both extracts evinced activity. Furthermore, C. orientalis leaf extract showed a concentration dependent inhibition of COX-1 pathway products 12-HHT and TXB2, reaching IC50 values below the lowest applied concentration (68.9% and 55.2% of 12-HHT and TXB2 production inhibition, respectively, at concentration of 0.4 mg/mL). Although inhibitors such as acetylsalicylic acid and quercetin showed higher activity, this study demonstrates that the investigated extracts are potential anti-inflammatory agents.
C3  - Natural Product Communications
T1  - Crataegus orientalis leaves and berries: Phenolic profiles, antioxidant and anti-inflammatory activity
IS  - 2
VL  - 12
DO  - 10.1177/1934578x1701200204
SP  - 159
EP  - 162
ER  - 

@conference{
author = "Šavikin, Katarina P. and Krstić Milošević, Dijana and Menković, Nebojša R. and Beara, Ivana N. and Mrkonjić, Zorica O. and Pljevljakušić, Dejan S.",
year = "2017",
abstract = "The present study was designed to define the phenolic content, antioxidant and anti-inflammatory activity of Crateagus orientalis Pall. Ex M. Bieb., traditionally used by local people in southern parts of F.Y.R. Macedonia. The presence and content of 7 phenolics in ethanolic extracts of leaves and berries were studied using HPLC-DAD, where the most dominant compounds were hyperoside, isoquercitrin and chlorogenic acid. The leaf extract was more effective as a DPPH radical scavenger (IC50 = 29.7 μg/g) than the berry extract, as well as in the relative reducing power on Fe3+. Anti-inflammatory potential was studied by means of cyclooxygenase-1 (COX-1) and 12-lipoxygenase (12-LOX) inhibitory activity; both extracts evinced activity. Furthermore, C. orientalis leaf extract showed a concentration dependent inhibition of COX-1 pathway products 12-HHT and TXB2, reaching IC50 values below the lowest applied concentration (68.9% and 55.2% of 12-HHT and TXB2 production inhibition, respectively, at concentration of 0.4 mg/mL). Although inhibitors such as acetylsalicylic acid and quercetin showed higher activity, this study demonstrates that the investigated extracts are potential anti-inflammatory agents.",
journal = "Natural Product Communications",
title = "Crataegus orientalis leaves and berries: Phenolic profiles, antioxidant and anti-inflammatory activity",
number = "2",
volume = "12",
doi = "10.1177/1934578x1701200204",
pages = "159-162"
}

Šavikin, K. P., Krstić Milošević, D., Menković, N. R., Beara, I. N., Mrkonjić, Z. O.,& Pljevljakušić, D. S.. (2017). Crataegus orientalis leaves and berries: Phenolic profiles, antioxidant and anti-inflammatory activity. in Natural Product Communications, 12(2), 159-162.
https://doi.org/10.1177/1934578x1701200204

Šavikin KP, Krstić Milošević D, Menković NR, Beara IN, Mrkonjić ZO, Pljevljakušić DS. Crataegus orientalis leaves and berries: Phenolic profiles, antioxidant and anti-inflammatory activity. in Natural Product Communications. 2017;12(2):159-162.
doi:10.1177/1934578x1701200204 .

Šavikin, Katarina P., Krstić Milošević, Dijana, Menković, Nebojša R., Beara, Ivana N., Mrkonjić, Zorica O., Pljevljakušić, Dejan S., "Crataegus orientalis leaves and berries: Phenolic profiles, antioxidant and anti-inflammatory activity" in Natural Product Communications, 12, no. 2 (2017):159-162,
https://doi.org/10.1177/1934578x1701200204 . .