TY  - JOUR
AU  - Morante-Carriel, Jaime
AU  - Živković, Suzana
AU  - Nájera, Hugo
AU  - Sellés-Marchart, Susana
AU  - Martínez-Márquez, Ascensión
AU  - Martínez-Esteso, María José
AU  - Obrebska, Anna
AU  - Samper-Herrero, Antonio
AU  - Bru-Martínez, Roque
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6697
AB  - Prenylated flavonoids (PFs) are natural flavonoids with a prenylated side chain attached to the flavonoid skeleton. They have great potential for biological activities such as anti-diabetic, anti-cancer, antimicrobial, antioxidant, anti-inflammatory, enzyme inhibition, and anti-Alzheimer’s effects. Medicinal chemists have recently paid increasing attention to PFs, which have become vital for developing new therapeutic agents. PFs have quickly developed through isolation and semi- or full synthesis, proving their high value in medicinal chemistry research. This review comprehensively summarizes the research progress of PFs, including natural PFs from the Moraceae family and their pharmacological activities. This information provides a basis for the selective design and optimization of multifunctional PF derivatives to treat multifactorial diseases.
PB  - Basel: MDPI
T2  - Plants
T1  - Prenylated Flavonoids of the Moraceae Family: A Comprehensive Review of Their Biological Activities
IS  - 9
VL  - 13
DO  - 10.3390/plants13091211
SP  - 1211
ER  - 

@article{
author = "Morante-Carriel, Jaime and Živković, Suzana and Nájera, Hugo and Sellés-Marchart, Susana and Martínez-Márquez, Ascensión and Martínez-Esteso, María José and Obrebska, Anna and Samper-Herrero, Antonio and Bru-Martínez, Roque",
year = "2024",
abstract = "Prenylated flavonoids (PFs) are natural flavonoids with a prenylated side chain attached to the flavonoid skeleton. They have great potential for biological activities such as anti-diabetic, anti-cancer, antimicrobial, antioxidant, anti-inflammatory, enzyme inhibition, and anti-Alzheimer’s effects. Medicinal chemists have recently paid increasing attention to PFs, which have become vital for developing new therapeutic agents. PFs have quickly developed through isolation and semi- or full synthesis, proving their high value in medicinal chemistry research. This review comprehensively summarizes the research progress of PFs, including natural PFs from the Moraceae family and their pharmacological activities. This information provides a basis for the selective design and optimization of multifunctional PF derivatives to treat multifactorial diseases.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Prenylated Flavonoids of the Moraceae Family: A Comprehensive Review of Their Biological Activities",
number = "9",
volume = "13",
doi = "10.3390/plants13091211",
pages = "1211"
}

Morante-Carriel, J., Živković, S., Nájera, H., Sellés-Marchart, S., Martínez-Márquez, A., Martínez-Esteso, M. J., Obrebska, A., Samper-Herrero, A.,& Bru-Martínez, R.. (2024). Prenylated Flavonoids of the Moraceae Family: A Comprehensive Review of Their Biological Activities. in Plants
Basel: MDPI., 13(9), 1211.
https://doi.org/10.3390/plants13091211

Morante-Carriel J, Živković S, Nájera H, Sellés-Marchart S, Martínez-Márquez A, Martínez-Esteso MJ, Obrebska A, Samper-Herrero A, Bru-Martínez R. Prenylated Flavonoids of the Moraceae Family: A Comprehensive Review of Their Biological Activities. in Plants. 2024;13(9):1211.
doi:10.3390/plants13091211 .

Morante-Carriel, Jaime, Živković, Suzana, Nájera, Hugo, Sellés-Marchart, Susana, Martínez-Márquez, Ascensión, Martínez-Esteso, María José, Obrebska, Anna, Samper-Herrero, Antonio, Bru-Martínez, Roque, "Prenylated Flavonoids of the Moraceae Family: A Comprehensive Review of Their Biological Activities" in Plants, 13, no. 9 (2024):1211,
https://doi.org/10.3390/plants13091211 . .

TY  - CONF
AU  - Škoro, Nikola
AU  - Jovanović, Olivera
AU  - Kumar, Amit
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Morina, Arian
AU  - Puač, Nevena
PY  - 2023
UR  - https://meetings.aps.org/Meeting/GEC23/Session/FF2.5
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6427
AB  - Non-thermal Plasmas (NTP), formed at atmospheric pressure, produce rich gas phase chemistry of Reactive Oxygen and Nitrogen Species (RONS). In a contact with a liquid target, NTPs induce production of different long-lived RONS species inside the liquid. NTPs can be employed for creation of plasma activated water (PAW) or for destruction of pollutants dissolved in water. In recent years both aspects are being developed as parts of a new field of plasma agricultural applications. The way to tailor the RONS concentrations is by adjusting of the plasma chemistry in the gas phase, the type of water and sample vessel properties. We will present results of plasma treatments of distilled and tap water using an atmospheric pressure plasma jet with pin electrode operating with addition of noble gas (He, Ar). The idea was to feature comparison of PAW properties obtained with different plasma system parameters. Produced PAW was used for treatments of different seeds and plants with an attention on linking the plasma properties with effects on treated plant material. On the other hand, the multi-pin jet was used for decontamination of water polluted by organic dyes and pharmaceuticals. This plasma source was designed based on a single-pin unit and obtained results can provide an insight into scaling up the plasma reactivity which is a necessary step for any agricultural application. We also tested variation in the efficiency of the plasma decontamination by adding recirculation of the contaminated sample. The effective treatment surface parameter proved to be significant for regulation of the decontamination level.
PB  - American Physical Society
C3  - The 76th Annual Gaseous Electronics Conference (GEC); 2023 Oct 9-13; Ann Arbor, USA
T1  - Use of atmospheric pressure plasma reactivity in water treatment for agricultural applications
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6427
ER  - 

@conference{
author = "Škoro, Nikola and Jovanović, Olivera and Kumar, Amit and Živković, Suzana and Milutinović, Milica and Morina, Arian and Puač, Nevena",
year = "2023",
abstract = "Non-thermal Plasmas (NTP), formed at atmospheric pressure, produce rich gas phase chemistry of Reactive Oxygen and Nitrogen Species (RONS). In a contact with a liquid target, NTPs induce production of different long-lived RONS species inside the liquid. NTPs can be employed for creation of plasma activated water (PAW) or for destruction of pollutants dissolved in water. In recent years both aspects are being developed as parts of a new field of plasma agricultural applications. The way to tailor the RONS concentrations is by adjusting of the plasma chemistry in the gas phase, the type of water and sample vessel properties. We will present results of plasma treatments of distilled and tap water using an atmospheric pressure plasma jet with pin electrode operating with addition of noble gas (He, Ar). The idea was to feature comparison of PAW properties obtained with different plasma system parameters. Produced PAW was used for treatments of different seeds and plants with an attention on linking the plasma properties with effects on treated plant material. On the other hand, the multi-pin jet was used for decontamination of water polluted by organic dyes and pharmaceuticals. This plasma source was designed based on a single-pin unit and obtained results can provide an insight into scaling up the plasma reactivity which is a necessary step for any agricultural application. We also tested variation in the efficiency of the plasma decontamination by adding recirculation of the contaminated sample. The effective treatment surface parameter proved to be significant for regulation of the decontamination level.",
publisher = "American Physical Society",
journal = "The 76th Annual Gaseous Electronics Conference (GEC); 2023 Oct 9-13; Ann Arbor, USA",
title = "Use of atmospheric pressure plasma reactivity in water treatment for agricultural applications",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6427"
}

Škoro, N., Jovanović, O., Kumar, A., Živković, S., Milutinović, M., Morina, A.,& Puač, N.. (2023). Use of atmospheric pressure plasma reactivity in water treatment for agricultural applications. in The 76th Annual Gaseous Electronics Conference (GEC); 2023 Oct 9-13; Ann Arbor, USA
American Physical Society..
https://hdl.handle.net/21.15107/rcub_ibiss_6427

Škoro N, Jovanović O, Kumar A, Živković S, Milutinović M, Morina A, Puač N. Use of atmospheric pressure plasma reactivity in water treatment for agricultural applications. in The 76th Annual Gaseous Electronics Conference (GEC); 2023 Oct 9-13; Ann Arbor, USA. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_6427 .

Škoro, Nikola, Jovanović, Olivera, Kumar, Amit, Živković, Suzana, Milutinović, Milica, Morina, Arian, Puač, Nevena, "Use of atmospheric pressure plasma reactivity in water treatment for agricultural applications" in The 76th Annual Gaseous Electronics Conference (GEC); 2023 Oct 9-13; Ann Arbor, USA (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_6427 .

TY  - CONF
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Mišić, Danijela
AU  - Nakarada, Đura
AU  - Mojović, Miloš
AU  - Jovanović, Olivera
AU  - Škoro, Nikola
AU  - Puač, Nevena
PY  - 2023
UR  - https://europlantbiology2023.org/wp-content/uploads/2023/07/PBE2023-Abstract-Book.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6224
AB  - Non-thermal plasma (NTP) technology offers a promising future in plant metabolic engineering, being energy efficient and Eco-friendly alternative to the conventional treatments [1]. Plasma environment is enriched with reactive oxygen and nitrogen species (RONS) that participate in various signaling pathways in plants by regulating their metabolic and developmental processes. In the present study calli of different carrot (Daucus carota L.) varieties was treated by using plasma needle device designed for biomedical applications [2]. Metabolite profiling revealed that plasma treatment could induce severe qualitative and quantitative changes of the major phenolic compounds detected in carrot calli. Current
metabolic alteration was followed by the significant shift in the antioxidant capacity of the treated calli. Obtained results outline the potential application of plasma treatment as a novel elicitor for the production of bio-active compounds in plant in vitro culture systems
PB  - French Society of Plant Biology
C3  - 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France
T1  - Alterations in specialized metabolites’ profile of Daucus carota L. Calli induced by low-temperature plasma treatment
SP  - 154
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6224
ER  - 

@conference{
author = "Živković, Suzana and Milutinović, Milica and Mišić, Danijela and Nakarada, Đura and Mojović, Miloš and Jovanović, Olivera and Škoro, Nikola and Puač, Nevena",
year = "2023",
abstract = "Non-thermal plasma (NTP) technology offers a promising future in plant metabolic engineering, being energy efficient and Eco-friendly alternative to the conventional treatments [1]. Plasma environment is enriched with reactive oxygen and nitrogen species (RONS) that participate in various signaling pathways in plants by regulating their metabolic and developmental processes. In the present study calli of different carrot (Daucus carota L.) varieties was treated by using plasma needle device designed for biomedical applications [2]. Metabolite profiling revealed that plasma treatment could induce severe qualitative and quantitative changes of the major phenolic compounds detected in carrot calli. Current
metabolic alteration was followed by the significant shift in the antioxidant capacity of the treated calli. Obtained results outline the potential application of plasma treatment as a novel elicitor for the production of bio-active compounds in plant in vitro culture systems",
publisher = "French Society of Plant Biology",
journal = "14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France",
title = "Alterations in specialized metabolites’ profile of Daucus carota L. Calli induced by low-temperature plasma treatment",
pages = "154",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6224"
}

Živković, S., Milutinović, M., Mišić, D., Nakarada, Đ., Mojović, M., Jovanović, O., Škoro, N.,& Puač, N.. (2023). Alterations in specialized metabolites’ profile of Daucus carota L. Calli induced by low-temperature plasma treatment. in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France
French Society of Plant Biology., 154.
https://hdl.handle.net/21.15107/rcub_ibiss_6224

Živković S, Milutinović M, Mišić D, Nakarada Đ, Mojović M, Jovanović O, Škoro N, Puač N. Alterations in specialized metabolites’ profile of Daucus carota L. Calli induced by low-temperature plasma treatment. in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France. 2023;:154.
https://hdl.handle.net/21.15107/rcub_ibiss_6224 .

Živković, Suzana, Milutinović, Milica, Mišić, Danijela, Nakarada, Đura, Mojović, Miloš, Jovanović, Olivera, Škoro, Nikola, Puač, Nevena, "Alterations in specialized metabolites’ profile of Daucus carota L. Calli induced by low-temperature plasma treatment" in 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France (2023):154,
https://hdl.handle.net/21.15107/rcub_ibiss_6224 .

TY  - JOUR
AU  - Gašić, Uroš
AU  - Banjanac, Tijana
AU  - Šiler, Branislav
AU  - Božunović, Jelena
AU  - Milutinović, Milica
AU  - Aničić, Neda
AU  - Dmitrović, Slavica
AU  - Skorić, Marijana
AU  - Nestorović Živković, Jasmina
AU  - Petrović, Luka
AU  - Todorović, Miloš
AU  - Živković, Suzana
AU  - Matekalo, Dragana
AU  - Filipović, Biljana
AU  - Lukić, Tamara
AU  - Mišić, Danijela
PY  - 2023
UR  - https://www.frontiersin.org/articles/10.3389/fpls.2023.1155297/full
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5494
AB  - The aim of this study was to determine intra- and interspecies variation in the qualitative and quantitative composition of methanol-soluble metabolites in the leaves of three Digitalis species ( D. lanata , D. ferruginea , and D. grandiflora ) from the central Balkans. Despite the steady use of foxglove constituents for human health as valuable medicinal products, populations of the genus Digitalis ( Plantaginaceae ) have been poorly investigated to describe their genetic and phenetic variation. Following untargeted profiling using UHPLC-LTQ Orbitrap MS, by which we identified a total of 115 compounds, 16 compounds were quantified using the UHPLC(–)HESI–QqQ-MS/MS approach. In total, 55 steroid compounds, 15 phenylethanoid glycosides, 27 flavonoids, and 14 phenolic acid derivatives were identified across the samples with D. lanata and D. ferruginea showing a great similarity, while 15 compounds were characteristic only for D. grandiflora . The phytochemical composition of methanol extracts, considered here as complex phenotypes, are further examined along multiple levels of biological organization (intra- and interpopulation) and subsequently subjected to chemometric data analysis. The quantitative composition of the selected set of 16 chemomarkers belonging to the classes of cardenolides (3 compounds) and phenolics (13 compounds) pointed to considerable differences between the taxa studied. D. grandiflora and D. ferruginea were found to be richer in phenolics as compared to cardenolides, which otherwise predominate in D. lanata over other compounds. PCA revealed lanatoside C, deslanoside, hispidulin, and p -coumaric acid to be the main compounds contributing to the differences between D. lanata on one side and D. grandiflora and D. ferruginea on the other, while p -coumaric acid, hispidulin, and digoxin contribute to the diversification between D. grandiflora and D. ferruginea . However, quantitative variation in the metabolite content within species was faint with mild population diversification visible in D. grandiflora and particularly in D. ferruginea. This pointed to the highly conserved content and ratio of targeted compounds within the analyzed species, which was not severely influenced by the geographic origin or environmental conditions. The presented metabolomics approach might have, along with morphometrics and molecular genetics studies, a high information value for further elucidation of the relationships among taxa within the genus Digitalis .
T2  - Frontiers in Plant Science
T1  - Variation in the chemical profiles of three foxglove species in the central Balkans
VL  - 14
DO  - 10.3389/fpls.2023.1155297
ER  - 

@article{
author = "Gašić, Uroš and Banjanac, Tijana and Šiler, Branislav and Božunović, Jelena and Milutinović, Milica and Aničić, Neda and Dmitrović, Slavica and Skorić, Marijana and Nestorović Živković, Jasmina and Petrović, Luka and Todorović, Miloš and Živković, Suzana and Matekalo, Dragana and Filipović, Biljana and Lukić, Tamara and Mišić, Danijela",
year = "2023",
abstract = "The aim of this study was to determine intra- and interspecies variation in the qualitative and quantitative composition of methanol-soluble metabolites in the leaves of three Digitalis species ( D. lanata , D. ferruginea , and D. grandiflora ) from the central Balkans. Despite the steady use of foxglove constituents for human health as valuable medicinal products, populations of the genus Digitalis ( Plantaginaceae ) have been poorly investigated to describe their genetic and phenetic variation. Following untargeted profiling using UHPLC-LTQ Orbitrap MS, by which we identified a total of 115 compounds, 16 compounds were quantified using the UHPLC(–)HESI–QqQ-MS/MS approach. In total, 55 steroid compounds, 15 phenylethanoid glycosides, 27 flavonoids, and 14 phenolic acid derivatives were identified across the samples with D. lanata and D. ferruginea showing a great similarity, while 15 compounds were characteristic only for D. grandiflora . The phytochemical composition of methanol extracts, considered here as complex phenotypes, are further examined along multiple levels of biological organization (intra- and interpopulation) and subsequently subjected to chemometric data analysis. The quantitative composition of the selected set of 16 chemomarkers belonging to the classes of cardenolides (3 compounds) and phenolics (13 compounds) pointed to considerable differences between the taxa studied. D. grandiflora and D. ferruginea were found to be richer in phenolics as compared to cardenolides, which otherwise predominate in D. lanata over other compounds. PCA revealed lanatoside C, deslanoside, hispidulin, and p -coumaric acid to be the main compounds contributing to the differences between D. lanata on one side and D. grandiflora and D. ferruginea on the other, while p -coumaric acid, hispidulin, and digoxin contribute to the diversification between D. grandiflora and D. ferruginea . However, quantitative variation in the metabolite content within species was faint with mild population diversification visible in D. grandiflora and particularly in D. ferruginea. This pointed to the highly conserved content and ratio of targeted compounds within the analyzed species, which was not severely influenced by the geographic origin or environmental conditions. The presented metabolomics approach might have, along with morphometrics and molecular genetics studies, a high information value for further elucidation of the relationships among taxa within the genus Digitalis .",
journal = "Frontiers in Plant Science",
title = "Variation in the chemical profiles of three foxglove species in the central Balkans",
volume = "14",
doi = "10.3389/fpls.2023.1155297"
}

Gašić, U., Banjanac, T., Šiler, B., Božunović, J., Milutinović, M., Aničić, N., Dmitrović, S., Skorić, M., Nestorović Živković, J., Petrović, L., Todorović, M., Živković, S., Matekalo, D., Filipović, B., Lukić, T.,& Mišić, D.. (2023). Variation in the chemical profiles of three foxglove species in the central Balkans. in Frontiers in Plant Science, 14.
https://doi.org/10.3389/fpls.2023.1155297

Gašić U, Banjanac T, Šiler B, Božunović J, Milutinović M, Aničić N, Dmitrović S, Skorić M, Nestorović Živković J, Petrović L, Todorović M, Živković S, Matekalo D, Filipović B, Lukić T, Mišić D. Variation in the chemical profiles of three foxglove species in the central Balkans. in Frontiers in Plant Science. 2023;14.
doi:10.3389/fpls.2023.1155297 .

Gašić, Uroš, Banjanac, Tijana, Šiler, Branislav, Božunović, Jelena, Milutinović, Milica, Aničić, Neda, Dmitrović, Slavica, Skorić, Marijana, Nestorović Živković, Jasmina, Petrović, Luka, Todorović, Miloš, Živković, Suzana, Matekalo, Dragana, Filipović, Biljana, Lukić, Tamara, Mišić, Danijela, "Variation in the chemical profiles of three foxglove species in the central Balkans" in Frontiers in Plant Science, 14 (2023),
https://doi.org/10.3389/fpls.2023.1155297 . .

TY  - JOUR
AU  - Todorović, Slađana
AU  - Perić, Marija
AU  - Nikolić, Biljana
AU  - Mandić, Boris
AU  - Cvetković, Stefana
AU  - Bogdanović, Milica
AU  - Živković, Suzana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5795
AB  - The aim of this study was to comparatively analyze chemical composition and biological activity of wild- and in vitro grown Rindera umbellata. Explants were cultivated on 0.003–0.3 M sucrose, fructose, or glucose. HPLC-DAD for quantifying rosmarinic (RA) and lithospermic B (LAB) acids and GC-MS/FID for qualitative pyrrolizidine alkaloids (PAs) detection were used. Antioxidant activity (DPPH and ABTS assays) and cytotoxicity (MTT test) were monitored. Identified PAs were 7-angeloyl heliotridane, lindelofine, 7-angeloyl heliotridine, 7-angeloyl-9-(+)-trachelanthylheliotridine, punctanecine, and heliosupine, with higher variability reported in wild-growing samples. Total phenolic contents (TPCs) were comparable in wild-growing and in vitro samples, but total flavonoid (TFC) and RA levels were multifold higher in in vitro samples. Notably, high concentration of LAB was detected in wild-growing roots. Amounts of 0.3 M and 0.1 M of sucrose were optimal for TFC and RA production, while maximal antioxidant activity was monitored in plants grown on 0.3 M sucrose. The MTT test indicated colorectal HT-29 as more sensitive than A549 lung adenocarcinoma and normal MRC-5 cells, showing selective sensitivity to wild-growing and 0.3 M sucrose samples. In conclusion, PAs in vitro, as well as TPC, TFC, RA, and LAB in both growing conditions were detected for the first time in R. umbellata.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Chemical Characterization, Antioxidant Activity, and Cytotoxity of Wild-Growing and In Vitro Cultivated Rindera umbellata (Waldst. and Kit.) Bunge
IS  - 3
VL  - 9
DO  - 10.3390/horticulturae9030381
SP  - 381
ER  - 

@article{
author = "Todorović, Slađana and Perić, Marija and Nikolić, Biljana and Mandić, Boris and Cvetković, Stefana and Bogdanović, Milica and Živković, Suzana",
year = "2023",
abstract = "The aim of this study was to comparatively analyze chemical composition and biological activity of wild- and in vitro grown Rindera umbellata. Explants were cultivated on 0.003–0.3 M sucrose, fructose, or glucose. HPLC-DAD for quantifying rosmarinic (RA) and lithospermic B (LAB) acids and GC-MS/FID for qualitative pyrrolizidine alkaloids (PAs) detection were used. Antioxidant activity (DPPH and ABTS assays) and cytotoxicity (MTT test) were monitored. Identified PAs were 7-angeloyl heliotridane, lindelofine, 7-angeloyl heliotridine, 7-angeloyl-9-(+)-trachelanthylheliotridine, punctanecine, and heliosupine, with higher variability reported in wild-growing samples. Total phenolic contents (TPCs) were comparable in wild-growing and in vitro samples, but total flavonoid (TFC) and RA levels were multifold higher in in vitro samples. Notably, high concentration of LAB was detected in wild-growing roots. Amounts of 0.3 M and 0.1 M of sucrose were optimal for TFC and RA production, while maximal antioxidant activity was monitored in plants grown on 0.3 M sucrose. The MTT test indicated colorectal HT-29 as more sensitive than A549 lung adenocarcinoma and normal MRC-5 cells, showing selective sensitivity to wild-growing and 0.3 M sucrose samples. In conclusion, PAs in vitro, as well as TPC, TFC, RA, and LAB in both growing conditions were detected for the first time in R. umbellata.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Chemical Characterization, Antioxidant Activity, and Cytotoxity of Wild-Growing and In Vitro Cultivated Rindera umbellata (Waldst. and Kit.) Bunge",
number = "3",
volume = "9",
doi = "10.3390/horticulturae9030381",
pages = "381"
}

Todorović, S., Perić, M., Nikolić, B., Mandić, B., Cvetković, S., Bogdanović, M.,& Živković, S.. (2023). Chemical Characterization, Antioxidant Activity, and Cytotoxity of Wild-Growing and In Vitro Cultivated Rindera umbellata (Waldst. and Kit.) Bunge. in Horticulturae
Basel: MDPI., 9(3), 381.
https://doi.org/10.3390/horticulturae9030381

Todorović S, Perić M, Nikolić B, Mandić B, Cvetković S, Bogdanović M, Živković S. Chemical Characterization, Antioxidant Activity, and Cytotoxity of Wild-Growing and In Vitro Cultivated Rindera umbellata (Waldst. and Kit.) Bunge. in Horticulturae. 2023;9(3):381.
doi:10.3390/horticulturae9030381 .

Todorović, Slađana, Perić, Marija, Nikolić, Biljana, Mandić, Boris, Cvetković, Stefana, Bogdanović, Milica, Živković, Suzana, "Chemical Characterization, Antioxidant Activity, and Cytotoxity of Wild-Growing and In Vitro Cultivated Rindera umbellata (Waldst. and Kit.) Bunge" in Horticulturae, 9, no. 3 (2023):381,
https://doi.org/10.3390/horticulturae9030381 . .

TY  - JOUR
AU  - Milutinović, Milica
AU  - Nakarada, Đura
AU  - Božunović, Jelena
AU  - Todorović, Miloš
AU  - Gašić, Uroš
AU  - Živković, Suzana
AU  - Skorić, Marijana
AU  - Ivković, Đurđa
AU  - Savić, Jelena
AU  - Devrnja, Nina
AU  - Aničić, Neda
AU  - Banjanac, Tijana
AU  - Mojović, Miloš
AU  - Mišić, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5505
AB  - The present study provides, for the first time, a physicochemical and biochemical characterization of the redox processes associated with the ripening of Solanum dulcamara L. (bittersweet) berries. Electron Paramagnetic Resonance Spectroscopy (EPRS) and Imaging (EPRI) measurements of reactive oxygen species (ROS) were performed in parallel with the tissue-specific metabolic profiling of major antioxidants and assessment of antioxidant enzymes activity. Fruit transition from the mature green (MG) to ripe red (RR) stage involved changes in the qualitative and quantitative content of antioxidants and the associated cellular oxidation and peroxidation processes. The skin of bittersweet berries, which was the major source of antioxidants, exhibited the highest antioxidant potential against DPPH radicals and nitroxyl spin probe 3CP. The efficient enzymatic antioxidant system played a critical protective role against the deleterious effects of progressive oxidative stress during ripening. Here, we present the EPRI methodology to assess the redox status of fruits and to discriminate between the redox states of different tissues. Interestingly, the intracellular reoxidation of cell-permeable nitroxide probe 3CP was observed for the first time in fruits or any other plant tissue, and its intensity is herein proposed as a reliable indicator of oxidative stress during ripening. The described noninvasive EPRI technique has the potential to have broader application in the study of redox processes associated with the development, senescence, and postharvest storage of fruits, as well as other circumstances in which oxidative stress is implicated.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening
IS  - 2
VL  - 12
DO  - 10.3390/antiox12020346
SP  - 346
ER  - 

@article{
author = "Milutinović, Milica and Nakarada, Đura and Božunović, Jelena and Todorović, Miloš and Gašić, Uroš and Živković, Suzana and Skorić, Marijana and Ivković, Đurđa and Savić, Jelena and Devrnja, Nina and Aničić, Neda and Banjanac, Tijana and Mojović, Miloš and Mišić, Danijela",
year = "2023",
abstract = "The present study provides, for the first time, a physicochemical and biochemical characterization of the redox processes associated with the ripening of Solanum dulcamara L. (bittersweet) berries. Electron Paramagnetic Resonance Spectroscopy (EPRS) and Imaging (EPRI) measurements of reactive oxygen species (ROS) were performed in parallel with the tissue-specific metabolic profiling of major antioxidants and assessment of antioxidant enzymes activity. Fruit transition from the mature green (MG) to ripe red (RR) stage involved changes in the qualitative and quantitative content of antioxidants and the associated cellular oxidation and peroxidation processes. The skin of bittersweet berries, which was the major source of antioxidants, exhibited the highest antioxidant potential against DPPH radicals and nitroxyl spin probe 3CP. The efficient enzymatic antioxidant system played a critical protective role against the deleterious effects of progressive oxidative stress during ripening. Here, we present the EPRI methodology to assess the redox status of fruits and to discriminate between the redox states of different tissues. Interestingly, the intracellular reoxidation of cell-permeable nitroxide probe 3CP was observed for the first time in fruits or any other plant tissue, and its intensity is herein proposed as a reliable indicator of oxidative stress during ripening. The described noninvasive EPRI technique has the potential to have broader application in the study of redox processes associated with the development, senescence, and postharvest storage of fruits, as well as other circumstances in which oxidative stress is implicated.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening",
number = "2",
volume = "12",
doi = "10.3390/antiox12020346",
pages = "346"
}

Milutinović, M., Nakarada, Đ., Božunović, J., Todorović, M., Gašić, U., Živković, S., Skorić, M., Ivković, Đ., Savić, J., Devrnja, N., Aničić, N., Banjanac, T., Mojović, M.,& Mišić, D.. (2023). Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening. in Antioxidants
Basel: MDPI., 12(2), 346.
https://doi.org/10.3390/antiox12020346

Milutinović M, Nakarada Đ, Božunović J, Todorović M, Gašić U, Živković S, Skorić M, Ivković Đ, Savić J, Devrnja N, Aničić N, Banjanac T, Mojović M, Mišić D. Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening. in Antioxidants. 2023;12(2):346.
doi:10.3390/antiox12020346 .

Milutinović, Milica, Nakarada, Đura, Božunović, Jelena, Todorović, Miloš, Gašić, Uroš, Živković, Suzana, Skorić, Marijana, Ivković, Đurđa, Savić, Jelena, Devrnja, Nina, Aničić, Neda, Banjanac, Tijana, Mojović, Miloš, Mišić, Danijela, "Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening" in Antioxidants, 12, no. 2 (2023):346,
https://doi.org/10.3390/antiox12020346 . .

TY  - JOUR
AU  - Kumar, Amit
AU  - Škoro, Nikola
AU  - Gernjak, Wolfgang
AU  - Jovanović, Olivera
AU  - Petrović, Anđelija
AU  - Živković, Suzana
AU  - Lumbaque, Elisabeth Cuervo
AU  - Farré, Maria José
AU  - Puač, Nevena
PY  - 2023
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0048969722082985
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5355
AB  - In this study, cold atmospheric plasma (CAP) was explored as a novel advanced oxidation process (AOP) for water decontamination. Samples with high concentration aqueous solutions of Diclofenac sodium (DCF) and 4-Chlorobenzoic acid (pCBA) were treated by plasma systems. Atmospheric pressure plasma jets (APPJs) with a 1 pin-electrode and multi-needle electrodes (3 pins) configurations were used. The plasma generated using argon as working gas was touching a stationary liquid surface in the case of pin electrode-APPJ while for multi-needle electrodes-APPJ the liquid sample was flowing during treatment. In both configurations, a commercial RF power supply was used for plasma ignition. Measurement of electrical signals enabled precise determination of power delivered from the plasma to the sample. The optical emission spectroscopy (OES) of plasma confirmed the appearance of excited reactive species in the plasma, such as hydroxyl radicals and atomic oxygen which are considered to be key reactive species in AOPs for the degradation of organic pollutants. Treatments were conducted with two different volumes (5 mL and 250 mL) of contaminated water samples. The data acquired allowed calculation of degradation efficiency and energy yield for both plasma sources. When treated with pin-APPJ, almost complete degradation of 5 mL DCF occurred in 1 min with the initial concentration of 25 mg/L and 50 mg/L, whereas 5 mL pCBA almost degraded in 10 min at the initial concentration of 25 mg/L and 40 mg/L. The treatment results with multi-needle electrodes system confirmed that DCF almost completely degraded in 30 min and pCBA degraded about 24 % in 50 min. The maximum calculated energy yield for 50 % removal was 6465 mg/kWh after treatment of 250 mL of DCF aqueous solution utilizing the plasma recirculation technique. The measurements also provided an insight to the kinetics of DCF and pCBA degradation. Degradation products and pathways for DCF were determined using LC-MS measurements.
PB  - Elsevier B.V.
T2  - Science of The Total Environment
T1  - Degradation of diclofenac and 4-chlorobenzoic acid in aqueous solution by
cold atmospheric plasma source
VL  - 864
DO  - 10.1016/j.scitotenv.2022.161194
SP  - 161194
ER  - 

@article{
author = "Kumar, Amit and Škoro, Nikola and Gernjak, Wolfgang and Jovanović, Olivera and Petrović, Anđelija and Živković, Suzana and Lumbaque, Elisabeth Cuervo and Farré, Maria José and Puač, Nevena",
year = "2023",
abstract = "In this study, cold atmospheric plasma (CAP) was explored as a novel advanced oxidation process (AOP) for water decontamination. Samples with high concentration aqueous solutions of Diclofenac sodium (DCF) and 4-Chlorobenzoic acid (pCBA) were treated by plasma systems. Atmospheric pressure plasma jets (APPJs) with a 1 pin-electrode and multi-needle electrodes (3 pins) configurations were used. The plasma generated using argon as working gas was touching a stationary liquid surface in the case of pin electrode-APPJ while for multi-needle electrodes-APPJ the liquid sample was flowing during treatment. In both configurations, a commercial RF power supply was used for plasma ignition. Measurement of electrical signals enabled precise determination of power delivered from the plasma to the sample. The optical emission spectroscopy (OES) of plasma confirmed the appearance of excited reactive species in the plasma, such as hydroxyl radicals and atomic oxygen which are considered to be key reactive species in AOPs for the degradation of organic pollutants. Treatments were conducted with two different volumes (5 mL and 250 mL) of contaminated water samples. The data acquired allowed calculation of degradation efficiency and energy yield for both plasma sources. When treated with pin-APPJ, almost complete degradation of 5 mL DCF occurred in 1 min with the initial concentration of 25 mg/L and 50 mg/L, whereas 5 mL pCBA almost degraded in 10 min at the initial concentration of 25 mg/L and 40 mg/L. The treatment results with multi-needle electrodes system confirmed that DCF almost completely degraded in 30 min and pCBA degraded about 24 % in 50 min. The maximum calculated energy yield for 50 % removal was 6465 mg/kWh after treatment of 250 mL of DCF aqueous solution utilizing the plasma recirculation technique. The measurements also provided an insight to the kinetics of DCF and pCBA degradation. Degradation products and pathways for DCF were determined using LC-MS measurements.",
publisher = "Elsevier B.V.",
journal = "Science of The Total Environment",
title = "Degradation of diclofenac and 4-chlorobenzoic acid in aqueous solution by
cold atmospheric plasma source",
volume = "864",
doi = "10.1016/j.scitotenv.2022.161194",
pages = "161194"
}

Kumar, A., Škoro, N., Gernjak, W., Jovanović, O., Petrović, A., Živković, S., Lumbaque, E. C., Farré, M. J.,& Puač, N.. (2023). Degradation of diclofenac and 4-chlorobenzoic acid in aqueous solution by
cold atmospheric plasma source. in Science of The Total Environment
Elsevier B.V.., 864, 161194.
https://doi.org/10.1016/j.scitotenv.2022.161194

Kumar A, Škoro N, Gernjak W, Jovanović O, Petrović A, Živković S, Lumbaque EC, Farré MJ, Puač N. Degradation of diclofenac and 4-chlorobenzoic acid in aqueous solution by
cold atmospheric plasma source. in Science of The Total Environment. 2023;864:161194.
doi:10.1016/j.scitotenv.2022.161194 .

Kumar, Amit, Škoro, Nikola, Gernjak, Wolfgang, Jovanović, Olivera, Petrović, Anđelija, Živković, Suzana, Lumbaque, Elisabeth Cuervo, Farré, Maria José, Puač, Nevena, "Degradation of diclofenac and 4-chlorobenzoic acid in aqueous solution by
cold atmospheric plasma source" in Science of The Total Environment, 864 (2023):161194,
https://doi.org/10.1016/j.scitotenv.2022.161194 . .

TY  - CONF
AU  - Todorović, Miloš
AU  - Milutinović, Milica
AU  - Božunović, Jelena
AU  - Aničić, Neda
AU  - Petrović, Luka
AU  - Skorić, Marijana
AU  - Nestorović Živković, Jasmina
AU  - Banjanac, Tijana
AU  - Gašić, Uroš
AU  - Živković, Suzana
AU  - Lukić, Tamara
AU  - Dmitrović, Slavica
AU  - Matekalo, Dragana
AU  - Šiler, Branislav
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5280
AB  - Species of the genus Centaurium (family Gentianaceae), well-known medicinal plants, threatened in the nature, are a rich source of bioactive compounds that are of great interest for the pharmaceutical and biotech industry. While the phytochemical characterization was investigated in detail in roots and leaves, nobody has so far tackled the question about the specialized metabolites composition in the centaury flowers. In this work, using untargeted metabolomics approach, we examined phytochemical differences in flowers of two centaury species, Centaurium pulchellum (Sw.) Druce and Centaurium tenuiflorum (Hoffmanns. & Link) Fritsch, both displaying variability in flowers coloration, which grades from white to pink. UHPLC-Orbitrap MS characterization of methanol extracts in a negative ionization mode resulted in the detection of 82 compounds in total. The identified compounds represented six structurally distinct groups: phenolic acid aglycones and glycosides; iridoid glycosides and derivatives; flavonoid glycosides and aglycones; xantone glucosides and aglycones; hydorxycinnamic acid amides and other compounds. All compounds found were identified by exact mass search of their deprotonated molecule [M – H]–, MS2, MS3 and MS4 fragmentation behavior, as well as by comparison with the available literature. Flavonoid and xantone glycosides and aglycones are the main classes of metabolites identified, and their content was strongly associated with the coloration of the flowers. However, a clear insight into the molecular mechanism underlying the centaury flower coloration is still lacking. Herein, the ultimate
goal of our research is to comparatevely profile the changes in metabolome and transcriptome
between pink and white centaury flowers in order to pinpoint key compounds and genes controlling
the flower color formation.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Comparative metabolomics of two Centaurium species displaying variable flower coloration phenotypes
SP  - 145
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5280
ER  - 

@conference{
author = "Todorović, Miloš and Milutinović, Milica and Božunović, Jelena and Aničić, Neda and Petrović, Luka and Skorić, Marijana and Nestorović Živković, Jasmina and Banjanac, Tijana and Gašić, Uroš and Živković, Suzana and Lukić, Tamara and Dmitrović, Slavica and Matekalo, Dragana and Šiler, Branislav and Mišić, Danijela",
year = "2022",
abstract = "Species of the genus Centaurium (family Gentianaceae), well-known medicinal plants, threatened in the nature, are a rich source of bioactive compounds that are of great interest for the pharmaceutical and biotech industry. While the phytochemical characterization was investigated in detail in roots and leaves, nobody has so far tackled the question about the specialized metabolites composition in the centaury flowers. In this work, using untargeted metabolomics approach, we examined phytochemical differences in flowers of two centaury species, Centaurium pulchellum (Sw.) Druce and Centaurium tenuiflorum (Hoffmanns. & Link) Fritsch, both displaying variability in flowers coloration, which grades from white to pink. UHPLC-Orbitrap MS characterization of methanol extracts in a negative ionization mode resulted in the detection of 82 compounds in total. The identified compounds represented six structurally distinct groups: phenolic acid aglycones and glycosides; iridoid glycosides and derivatives; flavonoid glycosides and aglycones; xantone glucosides and aglycones; hydorxycinnamic acid amides and other compounds. All compounds found were identified by exact mass search of their deprotonated molecule [M – H]–, MS2, MS3 and MS4 fragmentation behavior, as well as by comparison with the available literature. Flavonoid and xantone glycosides and aglycones are the main classes of metabolites identified, and their content was strongly associated with the coloration of the flowers. However, a clear insight into the molecular mechanism underlying the centaury flower coloration is still lacking. Herein, the ultimate
goal of our research is to comparatevely profile the changes in metabolome and transcriptome
between pink and white centaury flowers in order to pinpoint key compounds and genes controlling
the flower color formation.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Comparative metabolomics of two Centaurium species displaying variable flower coloration phenotypes",
pages = "145",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5280"
}

Todorović, M., Milutinović, M., Božunović, J., Aničić, N., Petrović, L., Skorić, M., Nestorović Živković, J., Banjanac, T., Gašić, U., Živković, S., Lukić, T., Dmitrović, S., Matekalo, D., Šiler, B.,& Mišić, D.. (2022). Comparative metabolomics of two Centaurium species displaying variable flower coloration phenotypes. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 145.
https://hdl.handle.net/21.15107/rcub_ibiss_5280

Todorović M, Milutinović M, Božunović J, Aničić N, Petrović L, Skorić M, Nestorović Živković J, Banjanac T, Gašić U, Živković S, Lukić T, Dmitrović S, Matekalo D, Šiler B, Mišić D. Comparative metabolomics of two Centaurium species displaying variable flower coloration phenotypes. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:145.
https://hdl.handle.net/21.15107/rcub_ibiss_5280 .

Todorović, Miloš, Milutinović, Milica, Božunović, Jelena, Aničić, Neda, Petrović, Luka, Skorić, Marijana, Nestorović Živković, Jasmina, Banjanac, Tijana, Gašić, Uroš, Živković, Suzana, Lukić, Tamara, Dmitrović, Slavica, Matekalo, Dragana, Šiler, Branislav, Mišić, Danijela, "Comparative metabolomics of two Centaurium species displaying variable flower coloration phenotypes" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):145,
https://hdl.handle.net/21.15107/rcub_ibiss_5280 .

TY  - CONF
AU  - Petrović, Luka
AU  - Skorić, Marijana
AU  - Aničić, Neda
AU  - Todorović, Miloš
AU  - Matekalo, Dragana
AU  - Milutinović, Milica
AU  - Božunović, Jelena
AU  - Živković, Suzana
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Šiler, Branislav
AU  - Banjanac, Tijana
AU  - Lukić, Tamara
AU  - Filipović, Biljana
AU  - Gašić, Uroš
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5279
AB  - Nepeta nuda L. (family Lamiaceae) is a widespread species characterized by the prevalence of bioactive specialized metabolites from the groups of iridoids and phenolic. Analyzed plants were grown in vitro and in greenhouse from seeds collected from eastern Serbia (“Debeli Lug”), obtained from other research groups (“Rhodopes”, Bulgaria), or commercially purchased (“Jellito”, Germany). The chemical composition of N. nuda leaves was analyzed using the UHPLC/DAD/(±)HESI±МS2 method, targeted towards dominant iridoid compounds. Both in vitro and greenhouse grown plants are rich in iridoid glycoside 1,5,9-epideoxyloganic acid (1,5,9– eDLA), and among analysed accessons N. nuda “Debeli Lug” was pointed out as the most productive one. When it comes to iridoid aglycones, it is noticeable that N. nuda “Jelitto” biosynthetizes cis, trans- isomer of nepetalactone (NL) while N. nuda “Rhodopes” and N. nuda “Debeli Lug” possess cis,cis-NL. Relative expression of early biosynthetic genes- EBGs (NnGPPS, NnGES, NnG80, Nn8HGO), in plants grown in vitro, is much higher in N. nuda “Debeli Lug“ accession, compared to N. nuda “Rhodopes” and N.nuda “Jellito”. In greenhouse grown plants, there is no particular regularity when it comes to the expression of EBGs in three N. nuda accessions. NEPS enzymes seem to be one of the key factors determining the metabolic flux and productivity of N. nuda. While plants grown in vitro and in greenhouse both express NnNEPS2, only plants grown in vitro express NnNEPS1.2. It is also noticeable that N. nuda “Debeli Lug“ accession displays the highest NnNEPS2 expression, and the lowest NnNEPS1.2 expression.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Iridoid profiles and expression patterns of iridoid-related biosynthetic genes in three Nepeta nuda L. accessions
SP  - 144
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5279
ER  - 

@conference{
author = "Petrović, Luka and Skorić, Marijana and Aničić, Neda and Todorović, Miloš and Matekalo, Dragana and Milutinović, Milica and Božunović, Jelena and Živković, Suzana and Nestorović Živković, Jasmina and Dmitrović, Slavica and Šiler, Branislav and Banjanac, Tijana and Lukić, Tamara and Filipović, Biljana and Gašić, Uroš and Mišić, Danijela",
year = "2022",
abstract = "Nepeta nuda L. (family Lamiaceae) is a widespread species characterized by the prevalence of bioactive specialized metabolites from the groups of iridoids and phenolic. Analyzed plants were grown in vitro and in greenhouse from seeds collected from eastern Serbia (“Debeli Lug”), obtained from other research groups (“Rhodopes”, Bulgaria), or commercially purchased (“Jellito”, Germany). The chemical composition of N. nuda leaves was analyzed using the UHPLC/DAD/(±)HESI±МS2 method, targeted towards dominant iridoid compounds. Both in vitro and greenhouse grown plants are rich in iridoid glycoside 1,5,9-epideoxyloganic acid (1,5,9– eDLA), and among analysed accessons N. nuda “Debeli Lug” was pointed out as the most productive one. When it comes to iridoid aglycones, it is noticeable that N. nuda “Jelitto” biosynthetizes cis, trans- isomer of nepetalactone (NL) while N. nuda “Rhodopes” and N. nuda “Debeli Lug” possess cis,cis-NL. Relative expression of early biosynthetic genes- EBGs (NnGPPS, NnGES, NnG80, Nn8HGO), in plants grown in vitro, is much higher in N. nuda “Debeli Lug“ accession, compared to N. nuda “Rhodopes” and N.nuda “Jellito”. In greenhouse grown plants, there is no particular regularity when it comes to the expression of EBGs in three N. nuda accessions. NEPS enzymes seem to be one of the key factors determining the metabolic flux and productivity of N. nuda. While plants grown in vitro and in greenhouse both express NnNEPS2, only plants grown in vitro express NnNEPS1.2. It is also noticeable that N. nuda “Debeli Lug“ accession displays the highest NnNEPS2 expression, and the lowest NnNEPS1.2 expression.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Iridoid profiles and expression patterns of iridoid-related biosynthetic genes in three Nepeta nuda L. accessions",
pages = "144",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5279"
}

Petrović, L., Skorić, M., Aničić, N., Todorović, M., Matekalo, D., Milutinović, M., Božunović, J., Živković, S., Nestorović Živković, J., Dmitrović, S., Šiler, B., Banjanac, T., Lukić, T., Filipović, B., Gašić, U.,& Mišić, D.. (2022). Iridoid profiles and expression patterns of iridoid-related biosynthetic genes in three Nepeta nuda L. accessions. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 144.
https://hdl.handle.net/21.15107/rcub_ibiss_5279

Petrović L, Skorić M, Aničić N, Todorović M, Matekalo D, Milutinović M, Božunović J, Živković S, Nestorović Živković J, Dmitrović S, Šiler B, Banjanac T, Lukić T, Filipović B, Gašić U, Mišić D. Iridoid profiles and expression patterns of iridoid-related biosynthetic genes in three Nepeta nuda L. accessions. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:144.
https://hdl.handle.net/21.15107/rcub_ibiss_5279 .

Petrović, Luka, Skorić, Marijana, Aničić, Neda, Todorović, Miloš, Matekalo, Dragana, Milutinović, Milica, Božunović, Jelena, Živković, Suzana, Nestorović Živković, Jasmina, Dmitrović, Slavica, Šiler, Branislav, Banjanac, Tijana, Lukić, Tamara, Filipović, Biljana, Gašić, Uroš, Mišić, Danijela, "Iridoid profiles and expression patterns of iridoid-related biosynthetic genes in three Nepeta nuda L. accessions" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):144,
https://hdl.handle.net/21.15107/rcub_ibiss_5279 .

TY  - CONF
AU  - Gašić, Uroš
AU  - Lukić, Tamara
AU  - Todorović, Miloš
AU  - Petrović, Luka
AU  - Aničić, Neda
AU  - Milutinović, Milica
AU  - Božunović, Jelena
AU  - Nestorović Živković, Jasmina
AU  - Banjanac, Tijana
AU  - Matekalo, Dragana
AU  - Filipović, Biljana
AU  - Dmitrović, Slavica
AU  - Šiler, Branislav
AU  - Skorić, Marijana
AU  - Živković, Suzana
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5281
AB  - The aim of this research was to determine inter-species diversity in qualitative composition of methanol-soluble metabolites in leaves of three Digitalis species (D. lanata, D. ferruginea, and D. ambigua) from the central Balkans. Using the UHPLC-LTQ OrbiTrap MS technique, a total of 119 compounds were identified based on their monoisotopic masses, MSn fragmentation, and previously reported MS data. All indetified compounds were clustered into seven groups: steroidal glycosides (43 compounds), steroid aglycones (12 compounds), phenylethanoid glycosides (15 compounds), flavonoid glycosides (18 compounds), flavonoid aglycones (9 compounds), phenolic acid derivatives (14 compounds), and 8 compounds belonging to other classes. In addition to the expected cardiac glycosides, steroidal saponins and pregnane and furostanol glycosides were also abundant in the extracts of analysed Digitalis species. A detailed LC/MS qualitative analysis revealed very similar profiles of steroidal glycosides in extracts of D. lanata and D. ferruginea leaves, which were characterized by significant amounts of pharmaceutically important digoxin, deslanatoside, and lanatosides A, B, and C. On the other hand, these compounds were not detected in leaves of D. ambigua. The UHPLC-LTQ OrbiTrap MS method was proved to be simple, rapid, and accurate strategy for unambiguous taxonomic determination and chemical differentiation of Digitalis species. It also alowed the selection of high-resolution chemical markers for revealing inter- and intra-population variability of Digitalis species adopting targeted metabolomic approach, which is the course of our further work.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Metabolomic evaluation of three Digitalis species (fam. Plantaginaceae) using liquid chromatography with multistage mass spectrometry
SP  - 149
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5281
ER  - 

@conference{
author = "Gašić, Uroš and Lukić, Tamara and Todorović, Miloš and Petrović, Luka and Aničić, Neda and Milutinović, Milica and Božunović, Jelena and Nestorović Živković, Jasmina and Banjanac, Tijana and Matekalo, Dragana and Filipović, Biljana and Dmitrović, Slavica and Šiler, Branislav and Skorić, Marijana and Živković, Suzana and Mišić, Danijela",
year = "2022",
abstract = "The aim of this research was to determine inter-species diversity in qualitative composition of methanol-soluble metabolites in leaves of three Digitalis species (D. lanata, D. ferruginea, and D. ambigua) from the central Balkans. Using the UHPLC-LTQ OrbiTrap MS technique, a total of 119 compounds were identified based on their monoisotopic masses, MSn fragmentation, and previously reported MS data. All indetified compounds were clustered into seven groups: steroidal glycosides (43 compounds), steroid aglycones (12 compounds), phenylethanoid glycosides (15 compounds), flavonoid glycosides (18 compounds), flavonoid aglycones (9 compounds), phenolic acid derivatives (14 compounds), and 8 compounds belonging to other classes. In addition to the expected cardiac glycosides, steroidal saponins and pregnane and furostanol glycosides were also abundant in the extracts of analysed Digitalis species. A detailed LC/MS qualitative analysis revealed very similar profiles of steroidal glycosides in extracts of D. lanata and D. ferruginea leaves, which were characterized by significant amounts of pharmaceutically important digoxin, deslanatoside, and lanatosides A, B, and C. On the other hand, these compounds were not detected in leaves of D. ambigua. The UHPLC-LTQ OrbiTrap MS method was proved to be simple, rapid, and accurate strategy for unambiguous taxonomic determination and chemical differentiation of Digitalis species. It also alowed the selection of high-resolution chemical markers for revealing inter- and intra-population variability of Digitalis species adopting targeted metabolomic approach, which is the course of our further work.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Metabolomic evaluation of three Digitalis species (fam. Plantaginaceae) using liquid chromatography with multistage mass spectrometry",
pages = "149",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5281"
}

Gašić, U., Lukić, T., Todorović, M., Petrović, L., Aničić, N., Milutinović, M., Božunović, J., Nestorović Živković, J., Banjanac, T., Matekalo, D., Filipović, B., Dmitrović, S., Šiler, B., Skorić, M., Živković, S.,& Mišić, D.. (2022). Metabolomic evaluation of three Digitalis species (fam. Plantaginaceae) using liquid chromatography with multistage mass spectrometry. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 149.
https://hdl.handle.net/21.15107/rcub_ibiss_5281

Gašić U, Lukić T, Todorović M, Petrović L, Aničić N, Milutinović M, Božunović J, Nestorović Živković J, Banjanac T, Matekalo D, Filipović B, Dmitrović S, Šiler B, Skorić M, Živković S, Mišić D. Metabolomic evaluation of three Digitalis species (fam. Plantaginaceae) using liquid chromatography with multistage mass spectrometry. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:149.
https://hdl.handle.net/21.15107/rcub_ibiss_5281 .

Gašić, Uroš, Lukić, Tamara, Todorović, Miloš, Petrović, Luka, Aničić, Neda, Milutinović, Milica, Božunović, Jelena, Nestorović Živković, Jasmina, Banjanac, Tijana, Matekalo, Dragana, Filipović, Biljana, Dmitrović, Slavica, Šiler, Branislav, Skorić, Marijana, Živković, Suzana, Mišić, Danijela, "Metabolomic evaluation of three Digitalis species (fam. Plantaginaceae) using liquid chromatography with multistage mass spectrometry" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):149,
https://hdl.handle.net/21.15107/rcub_ibiss_5281 .

TY  - CONF
AU  - Puač, Nevena
AU  - Jovanović, Olivera
AU  - Petrović, Anđelija
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Malović, Gordana
AU  - Škoro, Nikola
PY  - 2022
UR  - https://meetings.aps.org/Meeting/GEC22/Session/EF3.1
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6323
AB  - The atmospheric pressure plasmas (APPs) are known to be rich in Reactive Oxygen and Nitrogen Species (RONS) and this rich chemistry is responsible for triggering of cell mechanisms in case of plant or human/animal cells. We can divide this influence in two groups: (1) RONS in gas phase; (2) RONS in liquid phase. Therefore, in order to better understand the reasons for triggered mechanisms and outcomes (better germination percentage and speed, breakout of dormancy, creation of embryos etc.) we need to know and be able to tailor the plasma chemistry both in gas and liquid phase. We have used several APPs for gas phase treatments of plant cells and also for production of Plasma Activated Water (PAW) in order to investigate the influence of liquid RONS chemistry on plant cells. Here we will present different APP sources that are used for production of PAW used for seed imbibition in germination process and direct treatment of meristematic plant cells. Dielectric Barrier Discharge type of APPJ was used for production of PAW, while the plasma needle type was used for direct treatment of meristematic plant cells. The main idea was to check if plasma treatments can be used as for breaking of dormancy and trigger mechanisms in cells even in a normally non-permissive conditions.
PB  - American Physical Society
C3  - The 75th Annual Gaseous Electronics Conference (GEC); 2011 Oct 3-7; Sendai, Japan
T1  - Role of atmospheric pressure plasma in triggering of cell mechanisms in plant cells
SP  - EF3.00001
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6323
ER  - 

@conference{
author = "Puač, Nevena and Jovanović, Olivera and Petrović, Anđelija and Živković, Suzana and Milutinović, Milica and Malović, Gordana and Škoro, Nikola",
year = "2022",
abstract = "The atmospheric pressure plasmas (APPs) are known to be rich in Reactive Oxygen and Nitrogen Species (RONS) and this rich chemistry is responsible for triggering of cell mechanisms in case of plant or human/animal cells. We can divide this influence in two groups: (1) RONS in gas phase; (2) RONS in liquid phase. Therefore, in order to better understand the reasons for triggered mechanisms and outcomes (better germination percentage and speed, breakout of dormancy, creation of embryos etc.) we need to know and be able to tailor the plasma chemistry both in gas and liquid phase. We have used several APPs for gas phase treatments of plant cells and also for production of Plasma Activated Water (PAW) in order to investigate the influence of liquid RONS chemistry on plant cells. Here we will present different APP sources that are used for production of PAW used for seed imbibition in germination process and direct treatment of meristematic plant cells. Dielectric Barrier Discharge type of APPJ was used for production of PAW, while the plasma needle type was used for direct treatment of meristematic plant cells. The main idea was to check if plasma treatments can be used as for breaking of dormancy and trigger mechanisms in cells even in a normally non-permissive conditions.",
publisher = "American Physical Society",
journal = "The 75th Annual Gaseous Electronics Conference (GEC); 2011 Oct 3-7; Sendai, Japan",
title = "Role of atmospheric pressure plasma in triggering of cell mechanisms in plant cells",
pages = "EF3.00001",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6323"
}

Puač, N., Jovanović, O., Petrović, A., Živković, S., Milutinović, M., Malović, G.,& Škoro, N.. (2022). Role of atmospheric pressure plasma in triggering of cell mechanisms in plant cells. in The 75th Annual Gaseous Electronics Conference (GEC); 2011 Oct 3-7; Sendai, Japan
American Physical Society., EF3.00001.
https://hdl.handle.net/21.15107/rcub_ibiss_6323

Puač N, Jovanović O, Petrović A, Živković S, Milutinović M, Malović G, Škoro N. Role of atmospheric pressure plasma in triggering of cell mechanisms in plant cells. in The 75th Annual Gaseous Electronics Conference (GEC); 2011 Oct 3-7; Sendai, Japan. 2022;:EF3.00001.
https://hdl.handle.net/21.15107/rcub_ibiss_6323 .

Puač, Nevena, Jovanović, Olivera, Petrović, Anđelija, Živković, Suzana, Milutinović, Milica, Malović, Gordana, Škoro, Nikola, "Role of atmospheric pressure plasma in triggering of cell mechanisms in plant cells" in The 75th Annual Gaseous Electronics Conference (GEC); 2011 Oct 3-7; Sendai, Japan (2022):EF3.00001,
https://hdl.handle.net/21.15107/rcub_ibiss_6323 .

TY  - JOUR
AU  - Božunović, Jelena
AU  - Milutinović, Milica
AU  - Aničić, Neda
AU  - Skorić, Marijana
AU  - Matekalo, Dragana
AU  - Živković, Suzana
AU  - Dragićević, Milan
AU  - Filipović, Biljana
AU  - Banjanac, Tijana
AU  - Petrović, Luka
AU  - Mišić, Danijela
PY  - 2022
UR  - https://www.frontiersin.org/articles/10.3389/fpls.2022.914138/full
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9260424
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5073
AB  - Secoiridoid glucosides (SGs) are monoterpenoids derived from the iridoid cyclopentane-C-pyran skeleton with β-D glucose linked at C1 position. Coordinated metabolic processes, such as biosynthesis and catabolism of SGs, ensure constitutive presence of these bitter tasting compounds in plant tissues, which plays a decisive role in the defense against pathogens and herbivores. These compounds are susceptible to hydrolysis mediated by enzymes β-glucosidases, and the resulting aglycones are subsequently directed toward different metabolic pathways in plants. Function of two β-D-glucosidases (named CeBGlu1 and CeBGlu2) from centaury (Centaurium erythraea Rafn; fam. Gentianaceae), belonging to the glycoside hydrolase 1 (GH1) family, was confirmed using in vitro assays with recombinant proteins, following their heterologous expression in E. coli and His-tag affinity purification. Although they show slightly differential substrate preference, both isoforms display high specificity toward SGs and the organ-specific distribution of transcripts was positively correlated with the content of SGs in diploid and tetraploid C. erythraea plants. Transient overexpression of CeBGlu1 and CeBGlu2 in C. erythraea leaves induced changes in metabolite profiles. The effectiveness of transgene overexpression has been altered by plant ploidy. UHPLC/DAD/(±)HESI - MS2 profiling of leaves of diploid and tetraploid C. erythraea genotypes revealed that the amounts of major SGs; sweroside, swertiamarin, and gentiopicrin was decreased in agroinfiltrated leaves, especially when CeBGlu1 and CeBGlu2 were co-expressed with transgene silencing suppressor p19. The work demonstrates that in planta metabolic engineering adopting transient overexpression of CeBGlu1 and CeBGlu2 is a suitable tool for the modulation of SGs content and glucosides/aglycones ratio, which might have substantial effects on overall phytochemistry of C. erythraea.
PB  - Lausanne: Frontiers Media S.A.
T2  - Frontiers in Plant Science
T1  - Functional Characterization of Genes Coding for Novel β-D-Glucosidases Involved in the Initial Step of Secoiridoid Glucosides Catabolism in Centaurium erythraea Rafn.
VL  - 13
DO  - 10.3389/fpls.2022.914138
SP  - 914138
ER  - 

@article{
author = "Božunović, Jelena and Milutinović, Milica and Aničić, Neda and Skorić, Marijana and Matekalo, Dragana and Živković, Suzana and Dragićević, Milan and Filipović, Biljana and Banjanac, Tijana and Petrović, Luka and Mišić, Danijela",
year = "2022",
abstract = "Secoiridoid glucosides (SGs) are monoterpenoids derived from the iridoid cyclopentane-C-pyran skeleton with β-D glucose linked at C1 position. Coordinated metabolic processes, such as biosynthesis and catabolism of SGs, ensure constitutive presence of these bitter tasting compounds in plant tissues, which plays a decisive role in the defense against pathogens and herbivores. These compounds are susceptible to hydrolysis mediated by enzymes β-glucosidases, and the resulting aglycones are subsequently directed toward different metabolic pathways in plants. Function of two β-D-glucosidases (named CeBGlu1 and CeBGlu2) from centaury (Centaurium erythraea Rafn; fam. Gentianaceae), belonging to the glycoside hydrolase 1 (GH1) family, was confirmed using in vitro assays with recombinant proteins, following their heterologous expression in E. coli and His-tag affinity purification. Although they show slightly differential substrate preference, both isoforms display high specificity toward SGs and the organ-specific distribution of transcripts was positively correlated with the content of SGs in diploid and tetraploid C. erythraea plants. Transient overexpression of CeBGlu1 and CeBGlu2 in C. erythraea leaves induced changes in metabolite profiles. The effectiveness of transgene overexpression has been altered by plant ploidy. UHPLC/DAD/(±)HESI - MS2 profiling of leaves of diploid and tetraploid C. erythraea genotypes revealed that the amounts of major SGs; sweroside, swertiamarin, and gentiopicrin was decreased in agroinfiltrated leaves, especially when CeBGlu1 and CeBGlu2 were co-expressed with transgene silencing suppressor p19. The work demonstrates that in planta metabolic engineering adopting transient overexpression of CeBGlu1 and CeBGlu2 is a suitable tool for the modulation of SGs content and glucosides/aglycones ratio, which might have substantial effects on overall phytochemistry of C. erythraea.",
publisher = "Lausanne: Frontiers Media S.A.",
journal = "Frontiers in Plant Science",
title = "Functional Characterization of Genes Coding for Novel β-D-Glucosidases Involved in the Initial Step of Secoiridoid Glucosides Catabolism in Centaurium erythraea Rafn.",
volume = "13",
doi = "10.3389/fpls.2022.914138",
pages = "914138"
}

Božunović, J., Milutinović, M., Aničić, N., Skorić, M., Matekalo, D., Živković, S., Dragićević, M., Filipović, B., Banjanac, T., Petrović, L.,& Mišić, D.. (2022). Functional Characterization of Genes Coding for Novel β-D-Glucosidases Involved in the Initial Step of Secoiridoid Glucosides Catabolism in Centaurium erythraea Rafn.. in Frontiers in Plant Science
Lausanne: Frontiers Media S.A.., 13, 914138.
https://doi.org/10.3389/fpls.2022.914138

Božunović J, Milutinović M, Aničić N, Skorić M, Matekalo D, Živković S, Dragićević M, Filipović B, Banjanac T, Petrović L, Mišić D. Functional Characterization of Genes Coding for Novel β-D-Glucosidases Involved in the Initial Step of Secoiridoid Glucosides Catabolism in Centaurium erythraea Rafn.. in Frontiers in Plant Science. 2022;13:914138.
doi:10.3389/fpls.2022.914138 .

Božunović, Jelena, Milutinović, Milica, Aničić, Neda, Skorić, Marijana, Matekalo, Dragana, Živković, Suzana, Dragićević, Milan, Filipović, Biljana, Banjanac, Tijana, Petrović, Luka, Mišić, Danijela, "Functional Characterization of Genes Coding for Novel β-D-Glucosidases Involved in the Initial Step of Secoiridoid Glucosides Catabolism in Centaurium erythraea Rafn." in Frontiers in Plant Science, 13 (2022):914138,
https://doi.org/10.3389/fpls.2022.914138 . .

TY  - JOUR
AU  - Škoro, Nikola
AU  - Živković, Suzana
AU  - Jevremović, Slađana
AU  - Puač, Nevena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4938
AB  - Herein, we present the effect of surface dielectric barrier discharge (SDBD) air cold plasma on regrowth of chrysanthemum synthetic seeds (synseeds) and subsequent plantlet development. The plasma system used in this study operates in air at the frequency of 50 Hz. The detailed electrical characterization of SDBD was shown, as well as air plasma emission spectra obtained by optical emission spectroscopy. The chrysanthemum synseeds (encapsulated shoot tips) were treated in air plasma for different treatment times (0, 5 or 10 min). Plasma treatment significantly improved the regrowth and whole plantlet development of chrysanthemum synseeds under aseptic (in vitro) and non-aseptic (ex vitro) conditions. We evaluated the effect of SDBD plasma on synseed germination of four chrysanthemum cultivars after direct sowing in soil. Germination of synseeds directly sowed in soil was cultivar-dependent and 1.6−3.7 fold higher after plasma treatment in comparison with untreated synseeds. The study showed a highly effective novel strategy for direct conversion of simple monolayer alginate chrysanthemum synseeds into entire plantlets by plasma pre-conversion treatment. This treatment reduced contamination and displayed a considerable ex vitro ability to convert clonally identical chrysanthemum plants.
PB  - Basel: MDPI
T2  - Plants
T1  - Treatment of Chrysanthemum Synthetic Seeds by Air SDBD Plasma
IS  - 7
VL  - 11
DO  - 10.3390/plants11070907
SP  - 907
ER  - 

@article{
author = "Škoro, Nikola and Živković, Suzana and Jevremović, Slađana and Puač, Nevena",
year = "2022",
abstract = "Herein, we present the effect of surface dielectric barrier discharge (SDBD) air cold plasma on regrowth of chrysanthemum synthetic seeds (synseeds) and subsequent plantlet development. The plasma system used in this study operates in air at the frequency of 50 Hz. The detailed electrical characterization of SDBD was shown, as well as air plasma emission spectra obtained by optical emission spectroscopy. The chrysanthemum synseeds (encapsulated shoot tips) were treated in air plasma for different treatment times (0, 5 or 10 min). Plasma treatment significantly improved the regrowth and whole plantlet development of chrysanthemum synseeds under aseptic (in vitro) and non-aseptic (ex vitro) conditions. We evaluated the effect of SDBD plasma on synseed germination of four chrysanthemum cultivars after direct sowing in soil. Germination of synseeds directly sowed in soil was cultivar-dependent and 1.6−3.7 fold higher after plasma treatment in comparison with untreated synseeds. The study showed a highly effective novel strategy for direct conversion of simple monolayer alginate chrysanthemum synseeds into entire plantlets by plasma pre-conversion treatment. This treatment reduced contamination and displayed a considerable ex vitro ability to convert clonally identical chrysanthemum plants.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Treatment of Chrysanthemum Synthetic Seeds by Air SDBD Plasma",
number = "7",
volume = "11",
doi = "10.3390/plants11070907",
pages = "907"
}

Škoro, N., Živković, S., Jevremović, S.,& Puač, N.. (2022). Treatment of Chrysanthemum Synthetic Seeds by Air SDBD Plasma. in Plants
Basel: MDPI., 11(7), 907.
https://doi.org/10.3390/plants11070907

Škoro N, Živković S, Jevremović S, Puač N. Treatment of Chrysanthemum Synthetic Seeds by Air SDBD Plasma. in Plants. 2022;11(7):907.
doi:10.3390/plants11070907 .

Škoro, Nikola, Živković, Suzana, Jevremović, Slađana, Puač, Nevena, "Treatment of Chrysanthemum Synthetic Seeds by Air SDBD Plasma" in Plants, 11, no. 7 (2022):907,
https://doi.org/10.3390/plants11070907 . .

TY  - CONF
AU  - Božunović, Jelena
AU  - Milutinović, Milica
AU  - Aničić, Neda
AU  - Živković, Suzana
AU  - Skorić, Marijana
AU  - Matekalo, Dragana
AU  - Dragićević, Milan
AU  - Filipović, Biljana
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5711
AB  - Кичица (Centaurium erythraea Rafn) представља богат извор фармаколошки активних специјализованих метаболита, од којих су најзаступљенији секоиридоиди. Биосинтеза и разградња секоиридоида код кичице су координисани процеси који обезбеђују конститутивно присуство ових материја у ткивима биљке. Скелет молекула секоиридоидних глукозида се заснива на циклопентан-C-пирану за који је, на позицији C1, везан молекул β-D глукозе. У реакцији секоиридоидних глукозида са хидролитичким ензимима ослобађају се одговарајући агликони који се даље могу укључити у метаболичке процесе биљке. Код кичице су уочене две варијанте ензима бета-глукозидаза које припадају ензимској породици глукозидних хидролаза 1 (GH1). Гени за ензиме CeBGLU1 и CeBGLU2 су хетеролого експримирани у организму домаћину (Escherichia coli), а након изолације и пречишћавања рекомбинантних протеина, урађени су in vitro ензиматски тестови за потврду фунције ензима. Обе изоформе ензима показују високу специфичност за секоиридоидне глукозиде као супстрат чиме је по први пут потврђена функција биљних бета-глукозидаза карактеристичних за ову групу једињења. Привремена експресија CeBGLU1 и CeBGLU2 у листовима кичице, техником агроинфилтрације, довела је до редукције садржаја секоиридоида у листoвима, у случајевима када су CeBGLU1 и CeBGLU2 ко-експримирани заједно са p19 супресором. На тај начин је додатно потврђена функција два ензима, за које је утврђено да каталишу почетни корак у катаболизму секоиридоидних глукоида код кичице.
AB  - Kičica (Centaurium erythraea Rafn) predstavlja bogat izvor farmakološki aktivnih specijalizovanih metabolita, od kojih su najzastupljeniji sekoiridoidi. Biosinteza i razgradnja sekoiridoida kod kičice su koordinisani procesi koji obezbeđuju konstitutivno prisustvo ovih materija u tkivima biljke. Skelet molekula sekoiridoidnih glukozida se zasniva na ciklopentan-C-piranu za koji je, na poziciji C1, vezan molekul β-D glukoze. U reakciji sekoiridoidnih glukozida sa hidrolitičkim enzimima oslobađaju se odgovarajući aglikoni koji se dalje mogu uključiti u metaboličke procese biljke. Kod kičice su uočene dve varijante enzima beta-glukozidaza koje pripadaju enzimskoj porodici glukozidnih hidrolaza 1 (GH1). Geni za enzime CeBGLU1 i CeBGLU2 su heterologo eksprimirani u organizmu domaćinu (Escherichia coli), a nakon izolacije i prečišćavanja rekombinantnih proteina, urađeni su in vitro enzimatski testovi za potvrdu funcije enzima. Obe izoforme enzima pokazuju visoku specifičnost za sekoiridoidne glukozide kao supstrat čime je po prvi put potvrđena funkcija biljnih beta-glukozidaza karakterističnih za ovu grupu jedinjenja. Privremena ekspresija CeBGLU1 i CeBGLU2 u listovima kičice, tehnikom agroinfiltracije, dovela je do redukcije sadržaja sekoiridoida u listovima, u slučajevima kada su CeBGLU1 i CeBGLU2 ko-eksprimirani zajedno sa p19 supresorom. Na taj način je dodatno potvrđena funkcija dva enzima, za koje je utvrđeno da katališu početni korak u katabolizmu sekoiridoidnih glukoida kod kičice.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Изолација и функционална карактеризација гена за бета- глукозидазу врсте Centaurium erythraea Rafn
T1  - Izolacija i funkcionalna karakterizacija gena za beta- glukozidazu vrste Centaurium erythraea Rafn
SP  - 34
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5711
ER  - 

@conference{
author = "Božunović, Jelena and Milutinović, Milica and Aničić, Neda and Živković, Suzana and Skorić, Marijana and Matekalo, Dragana and Dragićević, Milan and Filipović, Biljana and Mišić, Danijela",
year = "2022",
abstract = "Кичица (Centaurium erythraea Rafn) представља богат извор фармаколошки активних специјализованих метаболита, од којих су најзаступљенији секоиридоиди. Биосинтеза и разградња секоиридоида код кичице су координисани процеси који обезбеђују конститутивно присуство ових материја у ткивима биљке. Скелет молекула секоиридоидних глукозида се заснива на циклопентан-C-пирану за који је, на позицији C1, везан молекул β-D глукозе. У реакцији секоиридоидних глукозида са хидролитичким ензимима ослобађају се одговарајући агликони који се даље могу укључити у метаболичке процесе биљке. Код кичице су уочене две варијанте ензима бета-глукозидаза које припадају ензимској породици глукозидних хидролаза 1 (GH1). Гени за ензиме CeBGLU1 и CeBGLU2 су хетеролого експримирани у организму домаћину (Escherichia coli), а након изолације и пречишћавања рекомбинантних протеина, урађени су in vitro ензиматски тестови за потврду фунције ензима. Обе изоформе ензима показују високу специфичност за секоиридоидне глукозиде као супстрат чиме је по први пут потврђена функција биљних бета-глукозидаза карактеристичних за ову групу једињења. Привремена експресија CeBGLU1 и CeBGLU2 у листовима кичице, техником агроинфилтрације, довела је до редукције садржаја секоиридоида у листoвима, у случајевима када су CeBGLU1 и CeBGLU2 ко-експримирани заједно са p19 супресором. На тај начин је додатно потврђена функција два ензима, за које је утврђено да каталишу почетни корак у катаболизму секоиридоидних глукоида код кичице., Kičica (Centaurium erythraea Rafn) predstavlja bogat izvor farmakološki aktivnih specijalizovanih metabolita, od kojih su najzastupljeniji sekoiridoidi. Biosinteza i razgradnja sekoiridoida kod kičice su koordinisani procesi koji obezbeđuju konstitutivno prisustvo ovih materija u tkivima biljke. Skelet molekula sekoiridoidnih glukozida se zasniva na ciklopentan-C-piranu za koji je, na poziciji C1, vezan molekul β-D glukoze. U reakciji sekoiridoidnih glukozida sa hidrolitičkim enzimima oslobađaju se odgovarajući aglikoni koji se dalje mogu uključiti u metaboličke procese biljke. Kod kičice su uočene dve varijante enzima beta-glukozidaza koje pripadaju enzimskoj porodici glukozidnih hidrolaza 1 (GH1). Geni za enzime CeBGLU1 i CeBGLU2 su heterologo eksprimirani u organizmu domaćinu (Escherichia coli), a nakon izolacije i prečišćavanja rekombinantnih proteina, urađeni su in vitro enzimatski testovi za potvrdu funcije enzima. Obe izoforme enzima pokazuju visoku specifičnost za sekoiridoidne glukozide kao supstrat čime je po prvi put potvrđena funkcija biljnih beta-glukozidaza karakterističnih za ovu grupu jedinjenja. Privremena ekspresija CeBGLU1 i CeBGLU2 u listovima kičice, tehnikom agroinfiltracije, dovela je do redukcije sadržaja sekoiridoida u listovima, u slučajevima kada su CeBGLU1 i CeBGLU2 ko-eksprimirani zajedno sa p19 supresorom. Na taj način je dodatno potvrđena funkcija dva enzima, za koje je utvrđeno da katališu početni korak u katabolizmu sekoiridoidnih glukoida kod kičice.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Изолација и функционална карактеризација гена за бета- глукозидазу врсте Centaurium erythraea Rafn, Izolacija i funkcionalna karakterizacija gena za beta- glukozidazu vrste Centaurium erythraea Rafn",
pages = "34",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5711"
}

Božunović, J., Milutinović, M., Aničić, N., Živković, S., Skorić, M., Matekalo, D., Dragićević, M., Filipović, B.,& Mišić, D.. (2022). Изолација и функционална карактеризација гена за бета- глукозидазу врсте Centaurium erythraea Rafn. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 34.
https://hdl.handle.net/21.15107/rcub_ibiss_5711

Božunović J, Milutinović M, Aničić N, Živković S, Skorić M, Matekalo D, Dragićević M, Filipović B, Mišić D. Изолација и функционална карактеризација гена за бета- глукозидазу врсте Centaurium erythraea Rafn. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:34.
https://hdl.handle.net/21.15107/rcub_ibiss_5711 .

Božunović, Jelena, Milutinović, Milica, Aničić, Neda, Živković, Suzana, Skorić, Marijana, Matekalo, Dragana, Dragićević, Milan, Filipović, Biljana, Mišić, Danijela, "Изолација и функционална карактеризација гена за бета- глукозидазу врсте Centaurium erythraea Rafn" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):34,
https://hdl.handle.net/21.15107/rcub_ibiss_5711 .

TY  - CONF
AU  - Božunović, Jelena
AU  - Milutinović, Milica
AU  - Aničić, Neda
AU  - Živković, Suzana
AU  - Dragićević, Milan
AU  - Skorić, Marijana
AU  - Matekalo, Dragana
AU  - Filipović, Biljana
AU  - Petrović, Luka
AU  - Banjanac, Tijana
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5189
AB  - Centaurium erythraea Rafn, also known as common centaury, is a rich source of pharmacologically active specialized metabolites among which secoiridoid glucosides (SG) predominate. These compounds belong to the group of monoterpenoids, which are derived from the iridoid cyclopentan-C-pyran skeleton with β-D glucose linked at C1 position. Major SGs of C. erythraea (sweroside, swertiamarin, and gentiopicrin) are biosynthesized through general iridoid pathway via secologanin, and it has been recently proved that their catabolism starts with the deglycosylation step catalyzed by beta-glucosidases (BGlu). Two BGlu genes from C. erythraea (CeBGlu1 and CeBGlu2), with previously confirmed function, were transiently overexpressed in leaves of five month-old diploid and tetraploid centaury plants. Agroinfiltration of Agrobacterium tumefaciens GV3101 strain harboring genes of interest in TMV-based overexpression pJL-TRBO vector (pJL-TR-BO:CeBGlu1; pJL-TRBO:CeBGlu2) resulted in transient in planta overexpression of CeBGlu1 and Ce-BGlu2. Metabolic profiling of secoiridoids in leaves of diploid and tetraploid C. erythraea genotypes revealed that amounts of sweroside, swertiamarin, and gentiopicrin, were significantly reduced in agroinfiltrated leaves, especially when CeBGlu1 and CeBGlu2 were co-expressed with transgene silencing suppressor p19. Diploid plants displayed more pronounced decrease in SGs content than tetraploids. The effectiveness of gene overexpression was obviously altered by plant ploidy, and was under the control of post-transcriptional gene silencing mechanisms in the infiltrated tissues. Transgenes are more prone to transcriptional inactivation in C. erythraea tetraploids than in diploids. In conclusion, SGs-specific β-glucosidases could serve as a molecular target of biotechnological interest, in order to shape SG profiles of centaury and related economically important
species of the Gentianaceae family.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia
T1  - Transient overexpression of β-glucosidase in leaves of Centaurium erythraea Rafn reduces the content of secoiridoid glucosides
SP  - 109
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5189
ER  - 

@conference{
author = "Božunović, Jelena and Milutinović, Milica and Aničić, Neda and Živković, Suzana and Dragićević, Milan and Skorić, Marijana and Matekalo, Dragana and Filipović, Biljana and Petrović, Luka and Banjanac, Tijana and Mišić, Danijela",
year = "2022",
abstract = "Centaurium erythraea Rafn, also known as common centaury, is a rich source of pharmacologically active specialized metabolites among which secoiridoid glucosides (SG) predominate. These compounds belong to the group of monoterpenoids, which are derived from the iridoid cyclopentan-C-pyran skeleton with β-D glucose linked at C1 position. Major SGs of C. erythraea (sweroside, swertiamarin, and gentiopicrin) are biosynthesized through general iridoid pathway via secologanin, and it has been recently proved that their catabolism starts with the deglycosylation step catalyzed by beta-glucosidases (BGlu). Two BGlu genes from C. erythraea (CeBGlu1 and CeBGlu2), with previously confirmed function, were transiently overexpressed in leaves of five month-old diploid and tetraploid centaury plants. Agroinfiltration of Agrobacterium tumefaciens GV3101 strain harboring genes of interest in TMV-based overexpression pJL-TRBO vector (pJL-TR-BO:CeBGlu1; pJL-TRBO:CeBGlu2) resulted in transient in planta overexpression of CeBGlu1 and Ce-BGlu2. Metabolic profiling of secoiridoids in leaves of diploid and tetraploid C. erythraea genotypes revealed that amounts of sweroside, swertiamarin, and gentiopicrin, were significantly reduced in agroinfiltrated leaves, especially when CeBGlu1 and CeBGlu2 were co-expressed with transgene silencing suppressor p19. Diploid plants displayed more pronounced decrease in SGs content than tetraploids. The effectiveness of gene overexpression was obviously altered by plant ploidy, and was under the control of post-transcriptional gene silencing mechanisms in the infiltrated tissues. Transgenes are more prone to transcriptional inactivation in C. erythraea tetraploids than in diploids. In conclusion, SGs-specific β-glucosidases could serve as a molecular target of biotechnological interest, in order to shape SG profiles of centaury and related economically important
species of the Gentianaceae family.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia",
title = "Transient overexpression of β-glucosidase in leaves of Centaurium erythraea Rafn reduces the content of secoiridoid glucosides",
pages = "109",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5189"
}

Božunović, J., Milutinović, M., Aničić, N., Živković, S., Dragićević, M., Skorić, M., Matekalo, D., Filipović, B., Petrović, L., Banjanac, T.,& Mišić, D.. (2022). Transient overexpression of β-glucosidase in leaves of Centaurium erythraea Rafn reduces the content of secoiridoid glucosides. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 109.
https://hdl.handle.net/21.15107/rcub_ibiss_5189

Božunović J, Milutinović M, Aničić N, Živković S, Dragićević M, Skorić M, Matekalo D, Filipović B, Petrović L, Banjanac T, Mišić D. Transient overexpression of β-glucosidase in leaves of Centaurium erythraea Rafn reduces the content of secoiridoid glucosides. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. 2022;:109.
https://hdl.handle.net/21.15107/rcub_ibiss_5189 .

Božunović, Jelena, Milutinović, Milica, Aničić, Neda, Živković, Suzana, Dragićević, Milan, Skorić, Marijana, Matekalo, Dragana, Filipović, Biljana, Petrović, Luka, Banjanac, Tijana, Mišić, Danijela, "Transient overexpression of β-glucosidase in leaves of Centaurium erythraea Rafn reduces the content of secoiridoid glucosides" in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia (2022):109,
https://hdl.handle.net/21.15107/rcub_ibiss_5189 .

TY  - CHAP
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Skorić, Marijana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5761
AB  - Asplenium ceterach L. (family Aspleniaceae) is a perennial herbaceous fern. The lower surface of its fronds is covered with scales, which over time mature to a rusty color, giving the plant a characteristic appearance, hence the common name “rustyback” fern. It inhabits almost all of Europe, including the Mediterranean region, and Central Asia to the Himalayas, usually occurring in cracks in rocks. Rustyback fern is belonging to resurrection plants due to its ability to withstand desiccation and consequently completely recover after rehydration. It is thought that high concentrations of phenolic acids (mainly chlorogenic acid and caffeic acid) enable A. ceterach to reduce the damage of reactive oxygen species formed during dehydration. In addition to phenolic acids, the presence of other phenolic compounds is detected in A. ceterach including flavonoids, tannins, coumarins, and quinones. Phytochemical studies have shown that these phenolic compounds are mainly found in sporophytes of rustyback fern, while A. ceterach gametophyte is characterized predominantly by xanthones, primarily mangiferin and its glycoside. Additionally, rustyback fern represents a rich source of different volatile organic compounds, mainly lipid derivatives. Owing to the presence of various bioactive metabolites, it is not surprising that A. ceterach has long been known for its healing properties, and numerous studies have proven its various biological activities, including antimicrobial, antioxidant, cytotoxic, anticancer, diuretic, as well as anti-inflammatory properties.
PB  - Cham: Springer
T2  - Bioactive Compounds in Bryophytes and Pteridophytes. Reference Series in Phytochemistry
T1  - Phytochemicals and Biological Activities of Asplenium ceterach
DO  - 10.1007/978-3-030-97415-2_19-1
ER  - 

@inbook{
author = "Živković, Suzana and Milutinović, Milica and Skorić, Marijana",
year = "2022",
abstract = "Asplenium ceterach L. (family Aspleniaceae) is a perennial herbaceous fern. The lower surface of its fronds is covered with scales, which over time mature to a rusty color, giving the plant a characteristic appearance, hence the common name “rustyback” fern. It inhabits almost all of Europe, including the Mediterranean region, and Central Asia to the Himalayas, usually occurring in cracks in rocks. Rustyback fern is belonging to resurrection plants due to its ability to withstand desiccation and consequently completely recover after rehydration. It is thought that high concentrations of phenolic acids (mainly chlorogenic acid and caffeic acid) enable A. ceterach to reduce the damage of reactive oxygen species formed during dehydration. In addition to phenolic acids, the presence of other phenolic compounds is detected in A. ceterach including flavonoids, tannins, coumarins, and quinones. Phytochemical studies have shown that these phenolic compounds are mainly found in sporophytes of rustyback fern, while A. ceterach gametophyte is characterized predominantly by xanthones, primarily mangiferin and its glycoside. Additionally, rustyback fern represents a rich source of different volatile organic compounds, mainly lipid derivatives. Owing to the presence of various bioactive metabolites, it is not surprising that A. ceterach has long been known for its healing properties, and numerous studies have proven its various biological activities, including antimicrobial, antioxidant, cytotoxic, anticancer, diuretic, as well as anti-inflammatory properties.",
publisher = "Cham: Springer",
journal = "Bioactive Compounds in Bryophytes and Pteridophytes. Reference Series in Phytochemistry",
booktitle = "Phytochemicals and Biological Activities of Asplenium ceterach",
doi = "10.1007/978-3-030-97415-2_19-1"
}

Živković, S., Milutinović, M.,& Skorić, M.. (2022). Phytochemicals and Biological Activities of Asplenium ceterach. in Bioactive Compounds in Bryophytes and Pteridophytes. Reference Series in Phytochemistry
Cham: Springer..
https://doi.org/10.1007/978-3-030-97415-2_19-1

Živković S, Milutinović M, Skorić M. Phytochemicals and Biological Activities of Asplenium ceterach. in Bioactive Compounds in Bryophytes and Pteridophytes. Reference Series in Phytochemistry. 2022;.
doi:10.1007/978-3-030-97415-2_19-1 .

Živković, Suzana, Milutinović, Milica, Skorić, Marijana, "Phytochemicals and Biological Activities of Asplenium ceterach" in Bioactive Compounds in Bryophytes and Pteridophytes. Reference Series in Phytochemistry (2022),
https://doi.org/10.1007/978-3-030-97415-2_19-1 . .

TY  - CONF
AU  - Živković, Suzana
AU  - Jevremović, Slađana
AU  - Gašić, Uroš
AU  - Milutinović, Milica
AU  - Petrović, Zoran Lj.
AU  - Škoro, Nikola
AU  - Puač, Nevena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5268
AB  - The Plasma Agriculture is a new field of plasma applications where non-thermal (cold)
plasmas (NTPs) operating at atmospheric pressure are used as a tool in biotechnology for genetic
manipulation of plants, for micropropagation, for studies of plant metabolism and cellular
development or a commercial production of natural products that cannot be chemically synthesized.
NTPs have rich chemistry of Reactive Oxygen and Nitrogen Species (RONS) that are responsible
for triggering various mechanisms and effects in plant cells, such as the induction of somatic
embryogenesis, higher and faster seed germination, better water uptake or have an anti-bacterial and
anti-viral effects, etc. [1-3]. In the current study plant undifferentiated compact tissue (calli) of
Balkan endemic dwarf bearded iris (Iris reichenbachii Heuff.) was treated using a RF plasma
needle device operating with He as a working gas. The flow of He was kept constant at 1 slm and
the power deposited to the plasma was below 2 W. The plasma needle was positioned 3 mm above
the callus surface enabling direct contact between the active plasma volume and the surface of the
sample. We induced significant morphological alterations in structure of non-embryonic calli that
could be attributed to the enhanced cell division of the plant cells at the surface of the calli that was
in contact with plasma. The differentiation of the calli cells was stimulated by reactive species
created in gas phase of NTP. The morphological changes were then followed by the significant long
term alteration in specialized metabolite content in derived calli types. Our results implicate that
direct plasma treatment could serve as a significant elicitor of the production of specific metabolites
in dwarf bearded iris calli.
PB  - Bratislava: FMFI UK
C3  - Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia
T1  - Long term effects in dwarf bearded iris (Iris reichenbachii Heuff.) calli metabolism induced by plasma treatment
SP  - 137
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5268
ER  - 

@conference{
author = "Živković, Suzana and Jevremović, Slađana and Gašić, Uroš and Milutinović, Milica and Petrović, Zoran Lj. and Škoro, Nikola and Puač, Nevena",
year = "2022",
abstract = "The Plasma Agriculture is a new field of plasma applications where non-thermal (cold)
plasmas (NTPs) operating at atmospheric pressure are used as a tool in biotechnology for genetic
manipulation of plants, for micropropagation, for studies of plant metabolism and cellular
development or a commercial production of natural products that cannot be chemically synthesized.
NTPs have rich chemistry of Reactive Oxygen and Nitrogen Species (RONS) that are responsible
for triggering various mechanisms and effects in plant cells, such as the induction of somatic
embryogenesis, higher and faster seed germination, better water uptake or have an anti-bacterial and
anti-viral effects, etc. [1-3]. In the current study plant undifferentiated compact tissue (calli) of
Balkan endemic dwarf bearded iris (Iris reichenbachii Heuff.) was treated using a RF plasma
needle device operating with He as a working gas. The flow of He was kept constant at 1 slm and
the power deposited to the plasma was below 2 W. The plasma needle was positioned 3 mm above
the callus surface enabling direct contact between the active plasma volume and the surface of the
sample. We induced significant morphological alterations in structure of non-embryonic calli that
could be attributed to the enhanced cell division of the plant cells at the surface of the calli that was
in contact with plasma. The differentiation of the calli cells was stimulated by reactive species
created in gas phase of NTP. The morphological changes were then followed by the significant long
term alteration in specialized metabolite content in derived calli types. Our results implicate that
direct plasma treatment could serve as a significant elicitor of the production of specific metabolites
in dwarf bearded iris calli.",
publisher = "Bratislava: FMFI UK",
journal = "Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia",
title = "Long term effects in dwarf bearded iris (Iris reichenbachii Heuff.) calli metabolism induced by plasma treatment",
pages = "137",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5268"
}

Živković, S., Jevremović, S., Gašić, U., Milutinović, M., Petrović, Z. Lj., Škoro, N.,& Puač, N.. (2022). Long term effects in dwarf bearded iris (Iris reichenbachii Heuff.) calli metabolism induced by plasma treatment. in Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia
Bratislava: FMFI UK., 137.
https://hdl.handle.net/21.15107/rcub_ibiss_5268

Živković S, Jevremović S, Gašić U, Milutinović M, Petrović ZL, Škoro N, Puač N. Long term effects in dwarf bearded iris (Iris reichenbachii Heuff.) calli metabolism induced by plasma treatment. in Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia. 2022;:137.
https://hdl.handle.net/21.15107/rcub_ibiss_5268 .

Živković, Suzana, Jevremović, Slađana, Gašić, Uroš, Milutinović, Milica, Petrović, Zoran Lj., Škoro, Nikola, Puač, Nevena, "Long term effects in dwarf bearded iris (Iris reichenbachii Heuff.) calli metabolism induced by plasma treatment" in Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia (2022):137,
https://hdl.handle.net/21.15107/rcub_ibiss_5268 .

TY  - CONF
AU  - Milutinović, Milica
AU  - Jovanović, Olivera
AU  - Devrnja, Nina
AU  - Todorović, Miloš
AU  - Živković, Suzana
AU  - Savić, Jelena
AU  - Skorić, Marijana
AU  - Puač, Nevena
AU  - Škoro, Nikola
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5269
AB  - Non-thermal temperature plasmas (NTPs) have rich chemistry of Reactive Oxygen and Nitrogen Species (RONS) that are formed in gas phase and, in case of water treatment, in gas/liquid interface in liquid [1]. NTPs can be applied in direct treatments of plant samples or indirectly when treated water, called Plasma Activated Water (PAW), is used in treatments. In both cases RONS (short or long-living) are responsible for triggering various mechanisms and effects in plant cells. RONS have a dual role and a dose-dependent effect, they can regulate the normal physiological activities of plants as signaling molecules at the range of physiological concentration, and can trigger damage to lipids, proteins and DNA at too high or too low concentration. Plants integrate RONS with genetic, epigenetic and external signals to regulate developmental processes. RONS signaling is highly integrated with hormonal signaling networks, thereby allowing plants to adjust to environmental cues.
All organisms have adaptive responses to oxidative stress, with antioxidant enzymes (i.e. catalase, peroxidase, superoxide dismutase) being induced by changes in the levels of H2O2 or O2 •-, leading to the activation or silencing of genes encoding defensive enzymes and transcription factors [2]. Although various observations have led to the suggestion that cells have the means to sense RONS and to induce specific responses, the underlying mechanisms are still not fully understood. In addition, plants also have non-enzymatic systems known to remove RONS, which are important players in plant processes that use RONS-dependent signaling mechanisms [3]. In this work, we have shown, by using molecular approaches, how PAW treatment affect expression of genes coding for specialized metabolites and hormones, thus regulating development and stress responses in the model plant system Arabidopsis thaliana.
PB  - Bratislava: FMFI UK
C3  - Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia
T1  - Molecular response to PAW in model plant species
SP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5269
ER  - 

@conference{
author = "Milutinović, Milica and Jovanović, Olivera and Devrnja, Nina and Todorović, Miloš and Živković, Suzana and Savić, Jelena and Skorić, Marijana and Puač, Nevena and Škoro, Nikola",
year = "2022",
abstract = "Non-thermal temperature plasmas (NTPs) have rich chemistry of Reactive Oxygen and Nitrogen Species (RONS) that are formed in gas phase and, in case of water treatment, in gas/liquid interface in liquid [1]. NTPs can be applied in direct treatments of plant samples or indirectly when treated water, called Plasma Activated Water (PAW), is used in treatments. In both cases RONS (short or long-living) are responsible for triggering various mechanisms and effects in plant cells. RONS have a dual role and a dose-dependent effect, they can regulate the normal physiological activities of plants as signaling molecules at the range of physiological concentration, and can trigger damage to lipids, proteins and DNA at too high or too low concentration. Plants integrate RONS with genetic, epigenetic and external signals to regulate developmental processes. RONS signaling is highly integrated with hormonal signaling networks, thereby allowing plants to adjust to environmental cues.
All organisms have adaptive responses to oxidative stress, with antioxidant enzymes (i.e. catalase, peroxidase, superoxide dismutase) being induced by changes in the levels of H2O2 or O2 •-, leading to the activation or silencing of genes encoding defensive enzymes and transcription factors [2]. Although various observations have led to the suggestion that cells have the means to sense RONS and to induce specific responses, the underlying mechanisms are still not fully understood. In addition, plants also have non-enzymatic systems known to remove RONS, which are important players in plant processes that use RONS-dependent signaling mechanisms [3]. In this work, we have shown, by using molecular approaches, how PAW treatment affect expression of genes coding for specialized metabolites and hormones, thus regulating development and stress responses in the model plant system Arabidopsis thaliana.",
publisher = "Bratislava: FMFI UK",
journal = "Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia",
title = "Molecular response to PAW in model plant species",
pages = "96",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5269"
}

Milutinović, M., Jovanović, O., Devrnja, N., Todorović, M., Živković, S., Savić, J., Skorić, M., Puač, N.,& Škoro, N.. (2022). Molecular response to PAW in model plant species. in Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia
Bratislava: FMFI UK., 96.
https://hdl.handle.net/21.15107/rcub_ibiss_5269

Milutinović M, Jovanović O, Devrnja N, Todorović M, Živković S, Savić J, Skorić M, Puač N, Škoro N. Molecular response to PAW in model plant species. in Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia. 2022;:96.
https://hdl.handle.net/21.15107/rcub_ibiss_5269 .

Milutinović, Milica, Jovanović, Olivera, Devrnja, Nina, Todorović, Miloš, Živković, Suzana, Savić, Jelena, Skorić, Marijana, Puač, Nevena, Škoro, Nikola, "Molecular response to PAW in model plant species" in Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia (2022):96,
https://hdl.handle.net/21.15107/rcub_ibiss_5269 .

TY  - CONF
AU  - Milutinović, Milica
AU  - Nakarada, Đura
AU  - Božunović, Jelena
AU  - Gašić, Uroš
AU  - Živković, Suzana
AU  - Savić, Jelena
AU  - Skorić, Marijana
AU  - Devrnja, Nina
AU  - Banjanac, Tijana
AU  - Todorović, Miloš
AU  - Mojović, Miloš
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5270
AB  - Identifying plant species tolerant to hypoxia and determining mechanisms this tolerance is standing upon may aid in improving nutritional quality of fruits of waterlogging-intolerant crops. Berries of Solanum dulcamara L. (bittersweet), a Eurasian species belonging to the Solanaceae family that grows in both dry habitats and wetlands, represent a suitable hypoxia-tolerant model species to study redox processes in relation to fruit development. Using biochemical and physicochemical analyses, we examined differences in enzymatic and non-enzymatic components of the antioxidant system of S. dulcamara during the transition from mature green (MG) to ripe red (RR) developmental stage. By combining untargeted (UHPLC/Orbitrap MSn) and targeted (UHPLC-DAD MS2) metabolomics approaches, we revealed a significant change in polyphenolic profile of S. dulcamara fruits during fruit ripening. In order to improve knowledge on the link between metabolism and cell redox status, Electron Paramagnetic Resonance Spectroscopy (EPRS) and Imaging (EPRI) of reactive oxygen species (ROS) in intact fruits, fruit sections and extracts, were performed in parallel with the analysis of tissue-specific distribution of antioxidants and activity of antioxidant enzymes, which demonstrated alterations in the redox state and the increase of total antioxidant capacity of bittersweet berries during ripening, especially of outer layers (pericarp) of the fruits. This knowledge could provide important information to direct research on fruit quality improvement of flood-intolerant crops that are a close relatives of bittersweet, such as tomato and eggplant.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Tissue-specific distribution of antioxidants during ripening of Solanum dulcamara L. fruits: the redox state alterations
SP  - 126
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5270
ER  - 

@conference{
author = "Milutinović, Milica and Nakarada, Đura and Božunović, Jelena and Gašić, Uroš and Živković, Suzana and Savić, Jelena and Skorić, Marijana and Devrnja, Nina and Banjanac, Tijana and Todorović, Miloš and Mojović, Miloš and Mišić, Danijela",
year = "2022",
abstract = "Identifying plant species tolerant to hypoxia and determining mechanisms this tolerance is standing upon may aid in improving nutritional quality of fruits of waterlogging-intolerant crops. Berries of Solanum dulcamara L. (bittersweet), a Eurasian species belonging to the Solanaceae family that grows in both dry habitats and wetlands, represent a suitable hypoxia-tolerant model species to study redox processes in relation to fruit development. Using biochemical and physicochemical analyses, we examined differences in enzymatic and non-enzymatic components of the antioxidant system of S. dulcamara during the transition from mature green (MG) to ripe red (RR) developmental stage. By combining untargeted (UHPLC/Orbitrap MSn) and targeted (UHPLC-DAD MS2) metabolomics approaches, we revealed a significant change in polyphenolic profile of S. dulcamara fruits during fruit ripening. In order to improve knowledge on the link between metabolism and cell redox status, Electron Paramagnetic Resonance Spectroscopy (EPRS) and Imaging (EPRI) of reactive oxygen species (ROS) in intact fruits, fruit sections and extracts, were performed in parallel with the analysis of tissue-specific distribution of antioxidants and activity of antioxidant enzymes, which demonstrated alterations in the redox state and the increase of total antioxidant capacity of bittersweet berries during ripening, especially of outer layers (pericarp) of the fruits. This knowledge could provide important information to direct research on fruit quality improvement of flood-intolerant crops that are a close relatives of bittersweet, such as tomato and eggplant.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Tissue-specific distribution of antioxidants during ripening of Solanum dulcamara L. fruits: the redox state alterations",
pages = "126",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5270"
}

Milutinović, M., Nakarada, Đ., Božunović, J., Gašić, U., Živković, S., Savić, J., Skorić, M., Devrnja, N., Banjanac, T., Todorović, M., Mojović, M.,& Mišić, D.. (2022). Tissue-specific distribution of antioxidants during ripening of Solanum dulcamara L. fruits: the redox state alterations. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 126.
https://hdl.handle.net/21.15107/rcub_ibiss_5270

Milutinović M, Nakarada Đ, Božunović J, Gašić U, Živković S, Savić J, Skorić M, Devrnja N, Banjanac T, Todorović M, Mojović M, Mišić D. Tissue-specific distribution of antioxidants during ripening of Solanum dulcamara L. fruits: the redox state alterations. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:126.
https://hdl.handle.net/21.15107/rcub_ibiss_5270 .

Milutinović, Milica, Nakarada, Đura, Božunović, Jelena, Gašić, Uroš, Živković, Suzana, Savić, Jelena, Skorić, Marijana, Devrnja, Nina, Banjanac, Tijana, Todorović, Miloš, Mojović, Miloš, Mišić, Danijela, "Tissue-specific distribution of antioxidants during ripening of Solanum dulcamara L. fruits: the redox state alterations" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):126,
https://hdl.handle.net/21.15107/rcub_ibiss_5270 .

TY  - CONF
AU  - Puač, Nevena
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Jovanović, Olivera
AU  - Petrović, Anđelija
AU  - Malović, Gordana
AU  - Škoro, Nikola
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5271
AB  - The population growth together with constant climate changes represent a serious challenge for humankind. Additionally, the usage of the pesticides have created adverse effect on environment,
which in return impact even more agricultural production. In order to comply with the demands and
to adapt to the new conditions the farmers need to change or upgrade existing practices by employing
new technologies. As being a promising tools in application in medicine, non-thermal (cold) plasmas
(NTPs) are seen as a green alternative to conventional fertilizers in agriculture to improve yields,
increase size and robustness of plants and to reduce (or eliminate) the need for pesticides [1, 2].
NTPs have rich chemistry of Reactive Oxygen and Nitrogen Species (RONS) that are formed in gas
phase and, in case of water treatment, in gas/liquid interface in liquid [2, 3]. We can use NTPs in
direct treatments of seeds or plant cells where samples are in contact with plasma gas phase chemistry
or indirectly when treated water is applied to the biological samples. In both cases RONS (short or
long-living) are responsible for triggering various mechanisms and effects in plant cells. To better
understand the reasons for triggered mechanisms and outcomes (better germination percentage and
speed, breakout of dormancy, creation of embryos etc.) it is important to characterize the plasma
chemistry both in gas and liquid phase. We have used several atmospheric pressure plasma systems
in treatments of seeds and plant cells in order to investigate the mechanisms responsible for better
germination in seeds, enzyme response and formation of somatic embryos in non-permissive
conditions. The mechanisms investigated were linked with the chemistry of RONS created in gas
phase and/or deposited in liquid phase. Thus, we were able to acquire the data that can be used in
optimization of plasma treatment processes.
C3  - Book of Abstracts: 9th International Conference on Plasma Medicine (ICPM9);  2022 Jun 27 - Jul 1; Utrecht, The Netherlands
T1  - Plasma treatment of seeds and plant cells: role of reactive oxygen and nitrogen species in formation of plantlets and embryos in non-permissive conditions
SP  - 16
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5271
ER  - 

@conference{
author = "Puač, Nevena and Živković, Suzana and Milutinović, Milica and Jovanović, Olivera and Petrović, Anđelija and Malović, Gordana and Škoro, Nikola",
year = "2022",
abstract = "The population growth together with constant climate changes represent a serious challenge for humankind. Additionally, the usage of the pesticides have created adverse effect on environment,
which in return impact even more agricultural production. In order to comply with the demands and
to adapt to the new conditions the farmers need to change or upgrade existing practices by employing
new technologies. As being a promising tools in application in medicine, non-thermal (cold) plasmas
(NTPs) are seen as a green alternative to conventional fertilizers in agriculture to improve yields,
increase size and robustness of plants and to reduce (or eliminate) the need for pesticides [1, 2].
NTPs have rich chemistry of Reactive Oxygen and Nitrogen Species (RONS) that are formed in gas
phase and, in case of water treatment, in gas/liquid interface in liquid [2, 3]. We can use NTPs in
direct treatments of seeds or plant cells where samples are in contact with plasma gas phase chemistry
or indirectly when treated water is applied to the biological samples. In both cases RONS (short or
long-living) are responsible for triggering various mechanisms and effects in plant cells. To better
understand the reasons for triggered mechanisms and outcomes (better germination percentage and
speed, breakout of dormancy, creation of embryos etc.) it is important to characterize the plasma
chemistry both in gas and liquid phase. We have used several atmospheric pressure plasma systems
in treatments of seeds and plant cells in order to investigate the mechanisms responsible for better
germination in seeds, enzyme response and formation of somatic embryos in non-permissive
conditions. The mechanisms investigated were linked with the chemistry of RONS created in gas
phase and/or deposited in liquid phase. Thus, we were able to acquire the data that can be used in
optimization of plasma treatment processes.",
journal = "Book of Abstracts: 9th International Conference on Plasma Medicine (ICPM9);  2022 Jun 27 - Jul 1; Utrecht, The Netherlands",
title = "Plasma treatment of seeds and plant cells: role of reactive oxygen and nitrogen species in formation of plantlets and embryos in non-permissive conditions",
pages = "16",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5271"
}

Puač, N., Živković, S., Milutinović, M., Jovanović, O., Petrović, A., Malović, G.,& Škoro, N.. (2022). Plasma treatment of seeds and plant cells: role of reactive oxygen and nitrogen species in formation of plantlets and embryos in non-permissive conditions. in Book of Abstracts: 9th International Conference on Plasma Medicine (ICPM9);  2022 Jun 27 - Jul 1; Utrecht, The Netherlands, 16.
https://hdl.handle.net/21.15107/rcub_ibiss_5271

Puač N, Živković S, Milutinović M, Jovanović O, Petrović A, Malović G, Škoro N. Plasma treatment of seeds and plant cells: role of reactive oxygen and nitrogen species in formation of plantlets and embryos in non-permissive conditions. in Book of Abstracts: 9th International Conference on Plasma Medicine (ICPM9);  2022 Jun 27 - Jul 1; Utrecht, The Netherlands. 2022;:16.
https://hdl.handle.net/21.15107/rcub_ibiss_5271 .

Puač, Nevena, Živković, Suzana, Milutinović, Milica, Jovanović, Olivera, Petrović, Anđelija, Malović, Gordana, Škoro, Nikola, "Plasma treatment of seeds and plant cells: role of reactive oxygen and nitrogen species in formation of plantlets and embryos in non-permissive conditions" in Book of Abstracts: 9th International Conference on Plasma Medicine (ICPM9);  2022 Jun 27 - Jul 1; Utrecht, The Netherlands (2022):16,
https://hdl.handle.net/21.15107/rcub_ibiss_5271 .

TY  - CONF
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Gašić, Uroš
AU  - Ivanov, Marija
AU  - Ćirić, Ana
AU  - Banjanac, Tijana
AU  - Skorić, Marijana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5190
AB  - Methanol (ME) and dichloromethane (DCM) extracts of rustyback fern (Asplenium ceterach L.) sporophytes were analyzed for antioxidant properties by thin layer chromatography (TLC)-based
bioautography, using DPPH as detection reagent, and comparatively tested for their antimicrobial and antibiofilm activity. Bioautography assay indicated that rustyback fern ME extract had signif-
icant antioxidant potential and moderate α-amylase activity, in comparison to the other extract 
used in the study. Antibacterial and antifungal activity of plant extracts against 6 bacteria and 6 
fungi strains was assessed by microdilution method and the results are presented as minimal in-
hibitory concentration and minimal bactericidal/fungicidal concentration. Both extracts showed 
significant antibacterial and antifungal potential against all tested strains, although in variable 
degree. The Gram-negative Escherichia coli and Gram-positive Bacillus cereus were the most sen-
sitive bacteria, whereas the most sensitive fungus was yeast Candida krusei. Furthermore, tested 
extracts have shown significant capability to prevent Candida albicans biofilm establishment with
no significant difference between the two extracts examined, and inhibition range of 72%–74%.
The results of the present study highlight A. ceterach extracts as a significant source of bioactive
com-pounds, which support their potential use against diseases caused by multi-drug resistant
microorganisms.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia
T1  - Bioactivity of different Asplenium ceterach L. extracts
SP  - 128
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5190
ER  - 

@conference{
author = "Živković, Suzana and Milutinović, Milica and Gašić, Uroš and Ivanov, Marija and Ćirić, Ana and Banjanac, Tijana and Skorić, Marijana",
year = "2022",
abstract = "Methanol (ME) and dichloromethane (DCM) extracts of rustyback fern (Asplenium ceterach L.) sporophytes were analyzed for antioxidant properties by thin layer chromatography (TLC)-based
bioautography, using DPPH as detection reagent, and comparatively tested for their antimicrobial and antibiofilm activity. Bioautography assay indicated that rustyback fern ME extract had signif-
icant antioxidant potential and moderate α-amylase activity, in comparison to the other extract 
used in the study. Antibacterial and antifungal activity of plant extracts against 6 bacteria and 6 
fungi strains was assessed by microdilution method and the results are presented as minimal in-
hibitory concentration and minimal bactericidal/fungicidal concentration. Both extracts showed 
significant antibacterial and antifungal potential against all tested strains, although in variable 
degree. The Gram-negative Escherichia coli and Gram-positive Bacillus cereus were the most sen-
sitive bacteria, whereas the most sensitive fungus was yeast Candida krusei. Furthermore, tested 
extracts have shown significant capability to prevent Candida albicans biofilm establishment with
no significant difference between the two extracts examined, and inhibition range of 72%–74%.
The results of the present study highlight A. ceterach extracts as a significant source of bioactive
com-pounds, which support their potential use against diseases caused by multi-drug resistant
microorganisms.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia",
title = "Bioactivity of different Asplenium ceterach L. extracts",
pages = "128",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5190"
}

Živković, S., Milutinović, M., Gašić, U., Ivanov, M., Ćirić, A., Banjanac, T.,& Skorić, M.. (2022). Bioactivity of different Asplenium ceterach L. extracts. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 128.
https://hdl.handle.net/21.15107/rcub_ibiss_5190

Živković S, Milutinović M, Gašić U, Ivanov M, Ćirić A, Banjanac T, Skorić M. Bioactivity of different Asplenium ceterach L. extracts. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. 2022;:128.
https://hdl.handle.net/21.15107/rcub_ibiss_5190 .

Živković, Suzana, Milutinović, Milica, Gašić, Uroš, Ivanov, Marija, Ćirić, Ana, Banjanac, Tijana, Skorić, Marijana, "Bioactivity of different Asplenium ceterach L. extracts" in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia (2022):128,
https://hdl.handle.net/21.15107/rcub_ibiss_5190 .

TY  - JOUR
AU  - Dmitrović, Slavica
AU  - Dragićević, Milan
AU  - Savić, Jelena
AU  - Milutinović, Milica
AU  - Živković, Suzana
AU  - Maksimović, Vuk
AU  - Matekalo, Dragana
AU  - Perišić, Mirjana
AU  - Mišić, Danijela
PY  - 2021
UR  - https://doi.org/10.3390/plants10010142
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4232
AB  - Phosphinothricin (PPT) is one of the most widely used herbicides. PTT targets glutamine synthetase (GS) activity in plants, and its phytotoxicity is ascribed to ammonium accumulation and reactive oxygen species bursts, which drives rapid lipid peroxidation of cell membranes. In agricultural fields, PPT is extensively sprayed on plant foliage; however, a portion of the herbicide reaches the soil. According to the present study, PPT absorbed via roots can be phytotoxic to Arabidopsis, inducing more adverse effects in roots than in shoots. Alterations in plant physiology caused by 10 days expo-sure to herbicide via roots are reflected through growth suppression, reduced chlorophyll content, perturbations in the sugar and organic acid metabolism, modifications in the activities and abundances of GS, catalase, peroxidase, and superoxide dismutase. Antagonistic interaction of Nepeta rtanjensis essential oil (NrEO) and PPT, emphasizes the existence of complex control mechanisms at the transcriptional and posttranslational level, which result in the mitigation of PPT-induced ammonium toxicity and in providing more efficient antioxidant defense of plants. Simultaneous application of the two agents in the field cannot be recommended; however, NrEO might be considered as the PPT post-treatment for reducing harmful effects of herbicide residues in the soil on non-target plants.
PB  - MDPI AG
T2  - Plants
T1  - Antagonistic interaction between phosphinothricin and nepeta rtanjensis essential oil affected ammonium metabolism and antioxidant defense of arabidopsis grown in vitro
IS  - 1
VL  - 10
DO  - 10.3390/plants10010142
SP  - 1
EP  - 20
ER  - 

@article{
author = "Dmitrović, Slavica and Dragićević, Milan and Savić, Jelena and Milutinović, Milica and Živković, Suzana and Maksimović, Vuk and Matekalo, Dragana and Perišić, Mirjana and Mišić, Danijela",
year = "2021",
abstract = "Phosphinothricin (PPT) is one of the most widely used herbicides. PTT targets glutamine synthetase (GS) activity in plants, and its phytotoxicity is ascribed to ammonium accumulation and reactive oxygen species bursts, which drives rapid lipid peroxidation of cell membranes. In agricultural fields, PPT is extensively sprayed on plant foliage; however, a portion of the herbicide reaches the soil. According to the present study, PPT absorbed via roots can be phytotoxic to Arabidopsis, inducing more adverse effects in roots than in shoots. Alterations in plant physiology caused by 10 days expo-sure to herbicide via roots are reflected through growth suppression, reduced chlorophyll content, perturbations in the sugar and organic acid metabolism, modifications in the activities and abundances of GS, catalase, peroxidase, and superoxide dismutase. Antagonistic interaction of Nepeta rtanjensis essential oil (NrEO) and PPT, emphasizes the existence of complex control mechanisms at the transcriptional and posttranslational level, which result in the mitigation of PPT-induced ammonium toxicity and in providing more efficient antioxidant defense of plants. Simultaneous application of the two agents in the field cannot be recommended; however, NrEO might be considered as the PPT post-treatment for reducing harmful effects of herbicide residues in the soil on non-target plants.",
publisher = "MDPI AG",
journal = "Plants",
title = "Antagonistic interaction between phosphinothricin and nepeta rtanjensis essential oil affected ammonium metabolism and antioxidant defense of arabidopsis grown in vitro",
number = "1",
volume = "10",
doi = "10.3390/plants10010142",
pages = "1-20"
}

Dmitrović, S., Dragićević, M., Savić, J., Milutinović, M., Živković, S., Maksimović, V., Matekalo, D., Perišić, M.,& Mišić, D.. (2021). Antagonistic interaction between phosphinothricin and nepeta rtanjensis essential oil affected ammonium metabolism and antioxidant defense of arabidopsis grown in vitro. in Plants
MDPI AG., 10(1), 1-20.
https://doi.org/10.3390/plants10010142

Dmitrović S, Dragićević M, Savić J, Milutinović M, Živković S, Maksimović V, Matekalo D, Perišić M, Mišić D. Antagonistic interaction between phosphinothricin and nepeta rtanjensis essential oil affected ammonium metabolism and antioxidant defense of arabidopsis grown in vitro. in Plants. 2021;10(1):1-20.
doi:10.3390/plants10010142 .

Dmitrović, Slavica, Dragićević, Milan, Savić, Jelena, Milutinović, Milica, Živković, Suzana, Maksimović, Vuk, Matekalo, Dragana, Perišić, Mirjana, Mišić, Danijela, "Antagonistic interaction between phosphinothricin and nepeta rtanjensis essential oil affected ammonium metabolism and antioxidant defense of arabidopsis grown in vitro" in Plants, 10, no. 1 (2021):1-20,
https://doi.org/10.3390/plants10010142 . .

TY  - CONF
AU  - Puač, Nevena
AU  - Škoro, Nikola
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Jevremović, Slađana
AU  - Petrović, Zoran Lj.
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5415
AB  - In parallel with the plethora of biomedical applications that employ atmospheric pressure plasma systems, another field of plasma applications is growing – plasma agriculture [1]. Chemical species in plasma, with ample amounts of Reactive Oxygen and Nitrogen Species (RONS), are responsible for triggering various mechanisms and effects in plant cells. For example in treatment of seeds, the rich plasma chemistry changes the coat of the treated seed resulting in changes of wettability, better water uptake, an increased percentage and speed of germination [1-3]. Another application of atmospheric pressure plasmas is for treatments of plant calli In biological research and biotechnology the plant callus (pl. calli) is induced from plant tissue and it forms growing mass of plant meristematic cells. Plant callus is widely used in plant biology both for basic research and industrial production: in plant biotechnology
as a tool for genetic manipulation of plants, for micropropagation, for studies of plant metabolism and cellular development, commercial production of natural products that cannot be chemically synthesized etc.. Puač et al. have studied the influence of RONS on Daucus carota calli showing their long-term influence [4]. We have used plasma needle type of the atmospheric pressure plasma device for direct plasmas treatments of
plant calli. The operational frequency of the device was 13.56 MHz and working gas was helium. The flow of
helium was kept constant at 1 slm  The detailed characterization of the discharge was performed by optical emission
spectroscopy, mass spectroscopy and electrical measurements  The direct plasma treatment of the plant calli was
used to investigate the plasma-cell interactions and to follow the response of the plant tissue several hours and days
after the treatment  We have used calli of model plant (Daucus carota) and of plants with specific issues, like small
bearded irise (Iris reichenbachii) who mainly produce non regenerative calli that do not enter the process of somatic
embryogenesis (SE)  Firstly, in treatments of D.carota calli we found that the formation of SE can be initiated even
under non- permissive conditions. Similar effects were obtained with I. reichenbachii calli where the atmospheric
plasma treatment induced significant morphological and physiological changes in non-embryonic calli toward SE
formation that were followed also with the enhanced production of arabinogalactan proteins
PB  - Greifswald: Leibniz Institute for Plasma Science and Technology
C3  - Book of Abstracts: 3rd International Workshop on Plasma Agriculture: IWOPA2021; 2021 Mar 1-3; Greifswald, Germany
T1  - Changing the plant tissue morphology and physiology by plasma treatment
SP  - 14
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5415
ER  - 

@conference{
author = "Puač, Nevena and Škoro, Nikola and Živković, Suzana and Milutinović, Milica and Jevremović, Slađana and Petrović, Zoran Lj.",
year = "2021",
abstract = "In parallel with the plethora of biomedical applications that employ atmospheric pressure plasma systems, another field of plasma applications is growing – plasma agriculture [1]. Chemical species in plasma, with ample amounts of Reactive Oxygen and Nitrogen Species (RONS), are responsible for triggering various mechanisms and effects in plant cells. For example in treatment of seeds, the rich plasma chemistry changes the coat of the treated seed resulting in changes of wettability, better water uptake, an increased percentage and speed of germination [1-3]. Another application of atmospheric pressure plasmas is for treatments of plant calli In biological research and biotechnology the plant callus (pl. calli) is induced from plant tissue and it forms growing mass of plant meristematic cells. Plant callus is widely used in plant biology both for basic research and industrial production: in plant biotechnology
as a tool for genetic manipulation of plants, for micropropagation, for studies of plant metabolism and cellular development, commercial production of natural products that cannot be chemically synthesized etc.. Puač et al. have studied the influence of RONS on Daucus carota calli showing their long-term influence [4]. We have used plasma needle type of the atmospheric pressure plasma device for direct plasmas treatments of
plant calli. The operational frequency of the device was 13.56 MHz and working gas was helium. The flow of
helium was kept constant at 1 slm  The detailed characterization of the discharge was performed by optical emission
spectroscopy, mass spectroscopy and electrical measurements  The direct plasma treatment of the plant calli was
used to investigate the plasma-cell interactions and to follow the response of the plant tissue several hours and days
after the treatment  We have used calli of model plant (Daucus carota) and of plants with specific issues, like small
bearded irise (Iris reichenbachii) who mainly produce non regenerative calli that do not enter the process of somatic
embryogenesis (SE)  Firstly, in treatments of D.carota calli we found that the formation of SE can be initiated even
under non- permissive conditions. Similar effects were obtained with I. reichenbachii calli where the atmospheric
plasma treatment induced significant morphological and physiological changes in non-embryonic calli toward SE
formation that were followed also with the enhanced production of arabinogalactan proteins",
publisher = "Greifswald: Leibniz Institute for Plasma Science and Technology",
journal = "Book of Abstracts: 3rd International Workshop on Plasma Agriculture: IWOPA2021; 2021 Mar 1-3; Greifswald, Germany",
title = "Changing the plant tissue morphology and physiology by plasma treatment",
pages = "14",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5415"
}

Puač, N., Škoro, N., Živković, S., Milutinović, M., Jevremović, S.,& Petrović, Z. Lj.. (2021). Changing the plant tissue morphology and physiology by plasma treatment. in Book of Abstracts: 3rd International Workshop on Plasma Agriculture: IWOPA2021; 2021 Mar 1-3; Greifswald, Germany
Greifswald: Leibniz Institute for Plasma Science and Technology., 14.
https://hdl.handle.net/21.15107/rcub_ibiss_5415

Puač N, Škoro N, Živković S, Milutinović M, Jevremović S, Petrović ZL. Changing the plant tissue morphology and physiology by plasma treatment. in Book of Abstracts: 3rd International Workshop on Plasma Agriculture: IWOPA2021; 2021 Mar 1-3; Greifswald, Germany. 2021;:14.
https://hdl.handle.net/21.15107/rcub_ibiss_5415 .

Puač, Nevena, Škoro, Nikola, Živković, Suzana, Milutinović, Milica, Jevremović, Slađana, Petrović, Zoran Lj., "Changing the plant tissue morphology and physiology by plasma treatment" in Book of Abstracts: 3rd International Workshop on Plasma Agriculture: IWOPA2021; 2021 Mar 1-3; Greifswald, Germany (2021):14,
https://hdl.handle.net/21.15107/rcub_ibiss_5415 .

TY  - CONF
AU  - Belić, Maja
AU  - Zdravković-Korać, Snežana
AU  - Pavlović, Suzana
AU  - Živković, Suzana
AU  - Vinterhalter, Dragan
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5412
AB  - Light and gibberellins (GAs) are essential for the induction of somatic embryogenesis in spinach. In the present study, the influence of light of different spectral qualities on the induction of somatic embryogenesis from the apical root sections of spinach seedlings was tested. The explants grown under blue light (B, emission maximum at 460 nm), provided by Light-emitting diodes (LED), exhibited the highest regeneration frequency (57.8%±0.36) and the mean somatic embryo (SE) number per explant (5.02±0.04) for eight weeks of cultivation on SE-induction medium supplemented containing 20 μM α-naphthaleneacetic acid and 5 μM GA3. The explants cultivated under white (W, 300-720 nm) LED or light provided by fluorescent lamps (FL, 380-680) produced a two times lower number of SEs per explant, while the explants grown under red (R, 630 nm) LED light or in darkness (D) failed to regenerate SEs. Considering that light greatly affects the level of GAs in plant tissues, and GAs are indispensable for SE induction in spinach, the expression profiles of genes encoding the key enzymes that catalyze the final steps of bioactive GA biosynthesis (GA20-oxidase and GA3-oxidase) and inactivation (GA2-oxidase) were tested in the explants grown under B, W or FL light. Surprisingly, no significant differences in the expression levels of these genes were observed among the treatments during of SE induction, indicating that light triggers SE induction by other mechanism, not including alterations in the expression of the aforementioned genes. Further research is needed for better understanding of the mechanism of SE induction by light.
PB  - East Sarajevo : Faculty of Agriculture
PB  - eas
C3  - Book of Abstracts: XII International Scientific Agriculture Symposium "Agrosym 2021"; 2021 Oct 7-10; Jahorina, Bosnia and Herzegovina
T1  - Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)
SP  - 213
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5412
ER  - 

@conference{
author = "Belić, Maja and Zdravković-Korać, Snežana and Pavlović, Suzana and Živković, Suzana and Vinterhalter, Dragan and Ćalić, Dušica and Milojević, Jelena",
year = "2021",
abstract = "Light and gibberellins (GAs) are essential for the induction of somatic embryogenesis in spinach. In the present study, the influence of light of different spectral qualities on the induction of somatic embryogenesis from the apical root sections of spinach seedlings was tested. The explants grown under blue light (B, emission maximum at 460 nm), provided by Light-emitting diodes (LED), exhibited the highest regeneration frequency (57.8%±0.36) and the mean somatic embryo (SE) number per explant (5.02±0.04) for eight weeks of cultivation on SE-induction medium supplemented containing 20 μM α-naphthaleneacetic acid and 5 μM GA3. The explants cultivated under white (W, 300-720 nm) LED or light provided by fluorescent lamps (FL, 380-680) produced a two times lower number of SEs per explant, while the explants grown under red (R, 630 nm) LED light or in darkness (D) failed to regenerate SEs. Considering that light greatly affects the level of GAs in plant tissues, and GAs are indispensable for SE induction in spinach, the expression profiles of genes encoding the key enzymes that catalyze the final steps of bioactive GA biosynthesis (GA20-oxidase and GA3-oxidase) and inactivation (GA2-oxidase) were tested in the explants grown under B, W or FL light. Surprisingly, no significant differences in the expression levels of these genes were observed among the treatments during of SE induction, indicating that light triggers SE induction by other mechanism, not including alterations in the expression of the aforementioned genes. Further research is needed for better understanding of the mechanism of SE induction by light.",
publisher = "East Sarajevo : Faculty of Agriculture, eas",
journal = "Book of Abstracts: XII International Scientific Agriculture Symposium "Agrosym 2021"; 2021 Oct 7-10; Jahorina, Bosnia and Herzegovina",
title = "Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)",
pages = "213",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5412"
}

Belić, M., Zdravković-Korać, S., Pavlović, S., Živković, S., Vinterhalter, D., Ćalić, D.,& Milojević, J.. (2021). Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L). in Book of Abstracts: XII International Scientific Agriculture Symposium "Agrosym 2021"; 2021 Oct 7-10; Jahorina, Bosnia and Herzegovina
East Sarajevo : Faculty of Agriculture., 213.
https://hdl.handle.net/21.15107/rcub_ibiss_5412

Belić M, Zdravković-Korać S, Pavlović S, Živković S, Vinterhalter D, Ćalić D, Milojević J. Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L). in Book of Abstracts: XII International Scientific Agriculture Symposium "Agrosym 2021"; 2021 Oct 7-10; Jahorina, Bosnia and Herzegovina. 2021;:213.
https://hdl.handle.net/21.15107/rcub_ibiss_5412 .

Belić, Maja, Zdravković-Korać, Snežana, Pavlović, Suzana, Živković, Suzana, Vinterhalter, Dragan, Ćalić, Dušica, Milojević, Jelena, "Blue led light promotes somatic embryogenesis from the lateral roots sections of spinach (Spinacia oleracea L)" in Book of Abstracts: XII International Scientific Agriculture Symposium "Agrosym 2021"; 2021 Oct 7-10; Jahorina, Bosnia and Herzegovina (2021):213,
https://hdl.handle.net/21.15107/rcub_ibiss_5412 .

TY  - CONF
AU  - Živković, Suzana
AU  - Jevremović, Slađana
AU  - Gašić, Uroš
AU  - Milutinović, Milica
AU  - Puač, Nevena
AU  - Škoro, Nikola
AU  - Petrović, Zoran Lj
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4414
AB  - Expansion of the plasma agriculture and plasma medicine and the demand for precise and localized in vivo treatments of living cells and tissues resulted in fast development of various plasma devices that operate at atmospheric pressure [1,2]. Irises can be regenerated in vitro by process of somatic embryogenesis and/or organogenesis by formation of shoot or root meristems on calli. During the induction of regeneration process, three types of calli could be distinguished, two friable regenerative calli: white embryogenic and green organogenic and the most abundant yellow, compact, nodular type of non-regenerative calli, designed as non embryonic [3]. Due to its lack of morphogenetic response and/or their low regeneration potential, the regeneration of non-embryogenic iris calli is one of the greatest challenges in this field of investigation. In the current study plant undifferentiated compact tissue (calli) of Balkan endemic dwarf bearded iris (Iris reichenbachii Heuff.) was treated using a RF plasma needle device operating with He as a working gas and changes at morphological and biochemical level were investigated. The plasma needle was positioned 3mm above the callus surface enabling direct contact between the active plasma volume and the surface. Direct plasma treatment triggered significant morphological alterations in structure of non-embryonic calli. Observed changes could be attributed to the enhanced cell division of the plant cells at the surface of the compact calli and differentiation of friable calli type stimulated by reactive species formed in the low temperature plasma. Indicated morphological changes were followed by the significant alteration in secondary metabolites in derived different calli types. Our results implicate that direct plasma treatment could serve as a significant elicitor of secondary metabolites production in dwarf bearded iris calli.
PB  - National Institute for Lasers, Plasma and Radiation Physics
PB  - Alexandru Ioan Cuza University
PB  - Romanian Physical Society
C3  - 19th International conference on plasma physics and applications, CPPA 2021
T1  - Plasma-induced morphological and biochemical changes in dwarf bearded iris (Iris reichenbachii Heuff.) calli
SP  - 130
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4414
ER  - 

@conference{
author = "Živković, Suzana and Jevremović, Slađana and Gašić, Uroš and Milutinović, Milica and Puač, Nevena and Škoro, Nikola and Petrović, Zoran Lj",
year = "2021",
abstract = "Expansion of the plasma agriculture and plasma medicine and the demand for precise and localized in vivo treatments of living cells and tissues resulted in fast development of various plasma devices that operate at atmospheric pressure [1,2]. Irises can be regenerated in vitro by process of somatic embryogenesis and/or organogenesis by formation of shoot or root meristems on calli. During the induction of regeneration process, three types of calli could be distinguished, two friable regenerative calli: white embryogenic and green organogenic and the most abundant yellow, compact, nodular type of non-regenerative calli, designed as non embryonic [3]. Due to its lack of morphogenetic response and/or their low regeneration potential, the regeneration of non-embryogenic iris calli is one of the greatest challenges in this field of investigation. In the current study plant undifferentiated compact tissue (calli) of Balkan endemic dwarf bearded iris (Iris reichenbachii Heuff.) was treated using a RF plasma needle device operating with He as a working gas and changes at morphological and biochemical level were investigated. The plasma needle was positioned 3mm above the callus surface enabling direct contact between the active plasma volume and the surface. Direct plasma treatment triggered significant morphological alterations in structure of non-embryonic calli. Observed changes could be attributed to the enhanced cell division of the plant cells at the surface of the compact calli and differentiation of friable calli type stimulated by reactive species formed in the low temperature plasma. Indicated morphological changes were followed by the significant alteration in secondary metabolites in derived different calli types. Our results implicate that direct plasma treatment could serve as a significant elicitor of secondary metabolites production in dwarf bearded iris calli.",
publisher = "National Institute for Lasers, Plasma and Radiation Physics, Alexandru Ioan Cuza University, Romanian Physical Society",
journal = "19th International conference on plasma physics and applications, CPPA 2021",
title = "Plasma-induced morphological and biochemical changes in dwarf bearded iris (Iris reichenbachii Heuff.) calli",
pages = "130",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4414"
}

Živković, S., Jevremović, S., Gašić, U., Milutinović, M., Puač, N., Škoro, N.,& Petrović, Z. L.. (2021). Plasma-induced morphological and biochemical changes in dwarf bearded iris (Iris reichenbachii Heuff.) calli. in 19th International conference on plasma physics and applications, CPPA 2021
National Institute for Lasers, Plasma and Radiation Physics., 130.
https://hdl.handle.net/21.15107/rcub_ibiss_4414

Živković S, Jevremović S, Gašić U, Milutinović M, Puač N, Škoro N, Petrović ZL. Plasma-induced morphological and biochemical changes in dwarf bearded iris (Iris reichenbachii Heuff.) calli. in 19th International conference on plasma physics and applications, CPPA 2021. 2021;:130.
https://hdl.handle.net/21.15107/rcub_ibiss_4414 .

Živković, Suzana, Jevremović, Slađana, Gašić, Uroš, Milutinović, Milica, Puač, Nevena, Škoro, Nikola, Petrović, Zoran Lj, "Plasma-induced morphological and biochemical changes in dwarf bearded iris (Iris reichenbachii Heuff.) calli" in 19th International conference on plasma physics and applications, CPPA 2021 (2021):130,
https://hdl.handle.net/21.15107/rcub_ibiss_4414 .